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OLFML1 Gene

protein-coding   GIFtS: 48
GCID: GC11P007463

Olfactomedin-Like 1

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(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
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Aliases
Olfactomedin-Like 11 2
MVAL5642
UNQ5642
Olfactomedin-Like Protein 12

External Ids:    HGNC: 244731   Entrez Gene: 2832982   Ensembl: ENSG000001838017   UniProtKB: Q6UWY53   

Export aliases for OLFML1 gene to outside databases

Previous GC identifers: GC11P007472 GC11P007165


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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GeneCards Summary for OLFML1 Gene:
OLFML1 (olfactomedin-like 1) is a protein-coding gene. An important paralog of this gene is GLDN.


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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RefSeq DNA sequence at NCBI GenBank:
NC_000011.9  NT_009237.19  NC_018922.2  
Regulatory elements:
   Regulatory transcription factor binding sites in the OLFML1 gene promoter:
         NF-AT   Gfi-1   SRY   NF-AT4   NF-AT2   HSF2   FOXO1a   NF-AT3   FOXO1   NF-AT1   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidOLFML1 promoter sequence
   Search Chromatin IP Primers for OLFML1

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat OLFML1


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 11p15.4   Ensembl cytogenetic band:  11p15.4   HGNC cytogenetic band: 11p15

OLFML1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
OLFML1 gene location

GeneLoc information about chromosome 11         GeneLoc Exon Structure

GeneLoc location for GC11P007463:  view genomic region     (about GC identifiers)

Start:
7,506,600 bp from pter      End:
7,532,608 bp from pter
Size:
26,009 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, Cloud-Clone Corp., eBioscience, and/or antibodies-online,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, Cloud-Clone Corp., eBioscience, and/or antibodies-online, Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, Cloud-Clone Corp, antibodies-online, and/or others.)
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UniProtKB/Swiss-Prot: OLFL1_HUMAN, Q6UWY5 (See protein sequence)
Recommended Name: Olfactomedin-like protein 1 precursor  
Size: 402 amino acids; 45951 Da
Secondary accessions: B4DP03 Q569G4

Explore the universe of human proteins at neXtProt for OLFML1: NX_Q6UWY5

Explore proteomics data for OLFML1 at MOPED

Post-translational modifications: 

  • Highly N-glycosylated1
  • Glycosylation2 at Asn66, Asn138, Asn183
  • Modification sites at PhosphoSitePlus

  • See OLFML1 Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins: NP_940876.2  
    ENSEMBL proteins: 
     ENSP00000433455   ENSP00000332511   ENSP00000431558   ENSP00000434813  

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    antibodies-online peptides for OLFML1

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    antibodies-online antibodies for OLFML1 (6 products) 

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    Cloud-Clone Corp. CLIAs for OLFML1
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    antibodies-online kits for OLFML1 (4 products) 


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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    1 InterPro protein domain:
     IPR003112 Olfac-like

    Graphical View of Domain Structure for InterPro Entry Q6UWY5

    ProtoNet protein and cluster: Q6UWY5

    1 Blocks protein domain: IPB003112 Olfactomedin-like

    UniProtKB/Swiss-Prot: OLFL1_HUMAN, Q6UWY5
    Similarity: Contains 1 olfactomedin-like domain


    Find genes that share domains with OLFML1           About GenesLikeMe


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, Vector BioLabs and/or Addgene, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Flow cytometry from eBioscience, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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         Gene Ontology (GO): 1 molecular function term:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005515protein binding ----
         
    Find genes that share ontologies with OLFML1           About GenesLikeMe


    Phenotypes:
         1 MGI phenotypic allele for Olfml1 (no phenotypes)

    Find genes that share phenotypes with OLFML1           About GenesLikeMe

    Animal Models:
         MGI mouse knock-out Olfml1tm1Lex for OLFML1

       genOway: Develop your customized and physiologically relevant rodent model for OLFML1

    miRNA
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    hsa-miR-607 hsa-miR-516b hsa-miR-3166 hsa-miR-410 hsa-miR-3153 hsa-miR-29b-1*
    SwitchGear 3'UTR luciferase reporter plasmidOLFML1 3' UTR sequence
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    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Subcellular locations from UniProtKB/Swiss-Prot
    OLFL1_HUMAN, Q6UWY5: Secreted
    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    extracellular5
    endoplasmic reticulum2
    cytosol1
    golgi apparatus1
    lysosome1

    Gene Ontology (GO): 1 cellular component term:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005576extracellular region IEA--

    Find genes that share ontologies with OLFML1           About GenesLikeMe


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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        Custom Pathway & Disease-focused RT2 Profiler PCR Arrays for OLFML1
    Interactions:

        Search GeneGlobe Interaction Network for OLFML1

    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
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    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
      Browse compounds at ApexBio 

    Browse Tocris compounds for OLFML1 (OLFL1)



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, and/or Addgene, Primers from OriGene, and/or QIAGEN, Flow cytometry from eBioscience )
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    REFSEQ mRNAs for OLFML1 gene: 
    NM_198474.3  

    Unigene Cluster for OLFML1:

    Olfactomedin-like 1
    Hs.503500  [show with all ESTs]
    Unigene Representative Sequence: NM_198474
    5 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000530135 ENST00000329293(uc001mfi.3 uc010raz.2 uc010rba.2)
    ENST00000534244 ENST00000528308 ENST00000528758
    miRNA
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    6 qRT-PCR Assays for microRNAs that regulate OLFML1:
    hsa-miR-607 hsa-miR-516b hsa-miR-3166 hsa-miR-410 hsa-miR-3153 hsa-miR-29b-1*
    SwitchGear 3'UTR luciferase reporter plasmidOLFML1 3' UTR sequence
    Inhib. RNA
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    OriGene RNAi products in human, mouse, rat for OLFML1
    Predesigned siRNA for gene silencing in human, mouse, rat OLFML1
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    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat OLFML1
      QuantiTect SYBR Green Assays in human, mouse, rat OLFML1
      QuantiFast Probe-based Assays in human, mouse, rat OLFML1

    Additional mRNA sequence: 

    AK297778.1 AK298137.1 AY358591.1 BC035895.1 BC092488.1 BX647908.1 

    3 DOTS entries:

    DT.101981601  DT.100743511  DT.100811414 

    Selected AceView cDNA sequences (see all 64):

    AL701890 CR591365 AW305097 NM_198474 CR624488 N76979 AI131455 BF846695 
    BF002933 BC035895 AA346531 AY358591 C02489 AL543108 BF087325 BM678629 
    BX090164 AL701891 BX504189 AA378396 BX390365 BE140233 AW862317 AW852232 

    GeneLoc Exon Structure

    2 Alternative Splicing Database (ASD) splice patterns (SP) for OLFML1    About this scheme

    ExUns: 1a · 1b ^ 2a · 2b ^ 3
    SP1:                              
    SP2:                              


    ECgene alternative splicing isoforms for OLFML1

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
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    OLFML1 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: TCTGCACAAG
    OLFML1 Expression
    About this image


    OLFML1 expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database
     selected tissues (see all 4) fully expand
     
     NULL (Uncategorized)
             Mesoderm-like cells
     
     Bone (Muscoskeletal System)
             Zeugopod Epiphyseal End
     
     Brain (Nervous System)
             Myelinating Oligodendrocyte Cells Forebrain White Matter
     
     Ovary (Reproductive System)
    OLFML1 Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    OLFML1 Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.503500

    UniProtKB/Swiss-Prot: OLFL1_HUMAN, Q6UWY5
    Tissue specificity: Mainly expressed in the small intestine, liver, lung and heart

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    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for OLFML1

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
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    This gene was present in the common ancestor of chordates.

    Orthologs for OLFML1 gene from Selected species (see all 11)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Olfml11 , 5 olfactomedin-like 11, 5 84.43(n)1
    82.38(a)1
      7 (57.21 cM)5
    2441981  NM_172907.31  NP_766495.11 
     1075674465 
    chicken
    (Gallus gallus)
    Aves OLFML11 olfactomedin-like 1 60.47(n)
    52.56(a)
      428842  XM_426398.3  XP_426398.4 
    lizard
    (Anolis carolinensis)
    Reptilia OLFML16
    olfactomedin-like 1
    49(a)
    1 ↔ 1
    GL343237.1(1911402-1930640)
    tropical clawed frog
    (Xenopus tropicalis)
    Amphibia olfml11 olfactomedin-like 1 53.77(n)
    41.14(a)
      100497389  XM_002938036.2  XP_002938082.2 
    zebrafish
    (Danio rerio)
    Actinopterygii LOC1000030481 olfactomedin-like protein 1-like 52.03(n)
    43.9(a)
      100003048  XM_001342657.4  XP_001342693.3 


    ENSEMBL Gene Tree for OLFML1 (if available)
    TreeFam Gene Tree for OLFML1 (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
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    Paralogs for OLFML1 gene
    GLDN2  OLFM32  OLFML32  OLFML2A2  OLFM12  OLFM42  OLFM22  OLFML2B2  
    MYOC2  
    4 SIMAP similar genes for OLFML1 using alignment to 3 protein entries:     OLFL1_HUMAN (see all proteins):
    DKFZp686H1812    OLFML3    MYOC    OLFM1

    Find genes that share paralogs with OLFML1           About GenesLikeMe



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
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    Selected SNPs for OLFML1 (see all 646)    About this table                                 

    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 11 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs792767541,2
    C,F--7504629(+) GATACC/TGGGGT 1 -- us2k12Minor allele frequency- T:0.08WA NA 238
    rs762669491,2
    F--7504643(+) AAAGCG/AGAAGG 1 -- us2k12Minor allele frequency- A:0.07CSA WA 120
    rs1501828341,2
    C--7504717(+) AGCAAC/TGGTCA 1 -- us2k10--------
    rs1478823391,2
    --7504736(+) GGTTAC/TGTGTA 1 -- us2k10--------
    rs1414139721,2
    --7504769(+) ATTTCA/GTCCAG 1 -- us2k10--------
    rs607099721,2
    C--7504774(+) GTCCAG/ACTGCA 1 -- us2k13Minor allele frequency- A:0.20CSA WA EA 240
    rs1142120961,2
    C,F--7504783(+) CAGGTC/GTAGGT 1 -- us2k11Minor allele frequency- G:0.05WA 118
    rs39230921,2
    C,F,A,H--7504791(+) GGTGCG/AGCAGG 1 -- us2k19Minor allele frequency- A:0.11NS EA NA WA 636
    rs43635701,2
    C,F,A,H--7504815(+) CCTTAC/TCCACC 1 -- us2k113Minor allele frequency- T:0.48NA WA CSA EA 485
    rs43843691,2
    C,F,A,H--7504831(+) CCCACG/TGAGGA 1 -- us2k112Minor allele frequency- T:0.46NA WA CSA EA 377

    HapMap Linkage Disequilibrium report for OLFML1 (7506600 - 7532608 bp)

    Structural Variations
         Database of Genomic Variants (DGV) 4 variations for OLFML1:    About this table    
    Variant IDTypeSubtypePubMed ID
    nsv499766OTHER Inversion21111241
    nsv7215OTHER Inversion18451855
    esv259966OTHER Complex20981092
    esv259496OTHER Complex20981092

    Site Specific Mutation Identification with PCR Assays
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    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
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      --

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
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    PubMed articles for OLFML1 gene integrated from 10 sources:
    (articles sorted by number of sources associating them with OLFML1)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. hOLFML1, a novel secreted glycoprotein, enhances the proliferation of human cancer cell lines in vitro. (PubMed id 18708057)1, 2, 9 Wan B.... Han Z.G. (FEBS Lett. 2008)
    2. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S.... Malek J. (Genome Res. 2004)
    3. Signal peptide prediction based on analysis of experimentally verified cleavage sites. (PubMed id 15340161)1, 2 Zhang Z. and Henzel W.J. (Protein Sci. 2004)
    4. The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment. (PubMed id 12975309)1, 2 Clark H.F.... Gray A.M. (Genome Res. 2003)
    5. Diversification of transcriptional modulation: large-scale identification and characterization of putative alternative promoters of human genes. (PubMed id 16344560)1 Kimura K.... Sugano S. (Genome Res. 2006)
    6. Human chromosome 11 DNA sequence and analysis including novel gene identification. (PubMed id 16554811)2 Taylor T.D....Sakaki Y. (Nature 2006)
    7. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)2 Ota T.... Sugano S. (Nat. Genet. 2004)
    8. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)1 Strausberg R.L....Marra M.A. (Proc. Natl. Acad. Sci. U.S.A. 2002)

    (in PubMed, OMIM, and NCBI Bookshelf)
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     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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    Entrez Gene: 283298 HGNC: 24473 AceView: OLFML1 Ensembl:ENSG00000183801 euGenes: HUgn283298
    ECgene: OLFML1 H-InvDB: OLFML1

    (According to HUGE)
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      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
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    NameDescription
    PharmGKB entry for OLFML1 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
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    Patent Information for OLFML1 gene:
    Search GeneIP for patents involving OLFML1

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    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, eBioscience, antibodies-online, and/or others, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Addgene, Cell lines from GenScript, and ESI BIO, Flow cytometery from eBioscience, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
    Advanced Cell Diagnostics, Animal models from genOway)
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     Block miRNA regulation of human, mouse, rat OLFML1 using miScript Target Protectors SeqTarget long-range PCR primers for resequencing OLFML1
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