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OLFM4 Gene

protein-coding   GIFtS: 51

GC13P052500
olfactomedin 4
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
GC1 1, 2
GW112 1, 2, 3
KIAA4294 2
OLM4 2, 3
OTTHUMP00000018468 2
OTTHUMP00000018469 2
OlfD 1, 2
UNQ362 2
bA209J19.1 2
hGC-1 2, 3
hOLfD 2, 3
Descriptions
Antiapoptotic protein GW112 3
G-CSF-stimulated clone 1 protein 3
olfactomedin 4 2
External Ids
HGNC: 171901
Entrez Gene: 105622
UniProtKB: Q6UX063
Ensembl: ENSG000001028377
Search outside databases for aliases for OLFM4 gene

Previous GC identifer: GC13P051401

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for OLFM4:
This gene was originally cloned from human myeloblasts and found to be selectively expressed in
inflammed colonic epithelium. The protein encoded is a member of the olfactomedin-related protein
family. The exact function of this gene has not yet been determined. [provided by RefSeq]

UniProtKB/Swiss-Prot: OLFM4_HUMAN, Q6UX06
Function: An antiapoptotic factor that promotes tumor growth. Promotes proliferation of pancreatic
cancer cells by favoring the transition from the S to G2/M phase. Facilitates cell adhesion

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the OLFM4 gene  

Entrez Gene cytogenetic band: 13q21.1   Ensembl cytogenetic band:  13q21.1   HGNC cytogenetic band: 13q14

OLFM4 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 13         GeneLoc Exon Structure

GeneLoc location for GC13P052500:     (about GC identifiers)

Start:
52,500,960 bp from pter
End:
52,524,193 bp from pter
Size:
23,234 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000013.9  NT_024524.13  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: OLFM4_HUMAN, Q6UX06 (See protein sequence)
Recommended Name: Olfactomedin-4 precursor  
Size: 510 amino acids; 57280 Da
Subunit: Homomultimer; disulfide-linked. Interacts with NDUFA13. Interacts with cell surface
lectins (locutions ricinus communis agglutinin I, concanavalin A and wheat germ agglutinin) and
cadherin
Subcellular location: Secreted, extracellular space. Note=According to PubMed:15059901 found in
mitochondrion and nucleus
Sequence caution: Sequence=AAC72970.1; Type=Erroneous initiation; Note=Translation N-terminally
extended; Sequence=AAC72970.1; Type=Frameshift; Positions=Several;
Secondary accessions: O95362 Q5VWG0 Q86T22

Post-translational modifications:

  • N-glycosylated1


  • REFSEQ proteins: NP_006409.3  

    ENSEMBL proteins: 
    ENSP00000219022 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (OLFM4)
    Browse Abnova for Human Recombinant Proteins
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    2 Gene Ontology (GO) cellular component terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005576 extracellular region IEA--
    GO:0005615 extracellular space IEA--
    About this table

    Antibodies for OLFM4: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Sigma-Aldrich Antibodies for OLFM4
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (OLFM4), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (OLFM4)
    Search Novus for antibodies for OLFM4

    Assays for OLFM4: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    1 InterPro domain/family:
     IPR003112 Olfac-like


       GeneDecks  OLFM4 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q6UX06

    ProtoNet protein and cluster: Q6UX06

    1 Blocks protein family: IPB003112 Olfactomedin-like

    UniProtKB/Swiss-Prot: OLFM4_HUMAN, Q6UX06
    Domain: The olfactomedin-like domain is involved in the interaction with cadherin
    Similarity: Contains 1 olfactomedin-like domain

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (OLFM4)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_006418

                  Applied Biosystems Silencer® siRNAs for OLFM4

                  Sigma-Aldrich siRNA for OLFM4  
                         Sigma-Aldrich shRNA for OLFM4  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Browse Clone Ranger at Invitrogen for clones
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_006418
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_006418
                                     untagged cDNA clone in CMV expression vector: NM_006418 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_006418

    UniProtKB/Swiss-Prot: OLFM4_HUMAN, Q6UX06
    Function: An antiapoptotic factor that promotes tumor growth. Promotes proliferation of pancreatic
    cancer cells by favoring the transition from the S to G2/M phase. Facilitates cell adhesion

    1 Gene Ontology (GO) molecular function term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005515 protein binding IEA--
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section



    1 Interacting protein for OLFM4 (ENSP000002190223) via UniProtKB, MINT, and/or STRING
    InteractantInteraction Details
    GeneCardExternal ID(s)
    C3ENSP000002459073STRING (score=.789)
    About this table

    1 Gene Ontology (GO) biological process term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0007155 cell adhesion IEA--
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for OLFM4

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (OLFM4)
    Browse for Gene Knock-down Tools from Millipore
    Browse Abnova for Chimera RNAi Products
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_006418

                  Sigma-Aldrich siRNA for OLFM4  
                         Sigma-Aldrich shRNA for OLFM4  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_006418  

    REFSEQ mRNAs for OLFM4 gene: 

    NM_006418.3   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_006418  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_006418
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_006418
                                     untagged cDNA clone in CMV expression vector: NM_006418 

    Additional cDNA sequence: 

    AK000683.1 AK300947.1 AY358567.1 BC047740.1 BC117329.1 

    8 DOTS entries:

    DT.446263  DT.100785237  DT.91810361  DT.100785238  DT.120771779  DT.95293320  DT.86848031  DT.97787537 

    24/199 AceView cDNA sequences (see all 199 ):

    AI422584 AI699868 BF222239 NM_006418 BX483055 AI565909 AA417910 AI635039 
    AI812031 T35699 BX483950 AW513818 AI923293 AI799729 AI811793 T24449 
    T34936 BX482217 AI827323 AI420765 AI859018 AW572449 AI587610 AI625261 

    highest scoring ESTs for OLFM4:

    AA366895 AA372630 AA376957 AA377282 AA417910 AA418161 AA531507 AA553882 AA555066 AA582775 

    Unigene Cluster for OLFM4:

    Olfactomedin 4
    Hs.508113  [show with all ESTs]
    Unigene Representative Sequence: AY358567


    GeneLoc Exon Structure

    4 Alternative Splicing Database (ASD) splice patterns (SP) for OLFM4

    ExUns: 1a · 1b ^ 2 ^ 3 ^ 4a · 4b · 4c ^ 5a · 5b ^ 6a · 6b · 6c
    SP1:              -           -                                             
    SP2:              -           -           -     -     -     -               
    SP3:                          -                                             
    SP4:                                                                        

    About this scheme

    ECgene alternative splicing isoforms for OLFM4

    1 Ensembl transcript including schematic representation:
    ENST00000219022  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    OLFM4 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for OLFM4

    1 / 2 / 3

    3 probe-sets matching OLFM4 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
    38615_at2, 3 U95-A 1 1.00 1.00 1.00 1.00 AF097021 0.80 1.00 0.91 1

    212768_s_at2 U133-A 1 1.00 1.00 -- -- -- -- -- -- --

    212768_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    GeneDecks  OLFM4 for binary patterns associated with the probe-sets selected above  
    About GeneDecksing
    About this table    
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: TAATTTTTGC

    SOURCE GeneReport for Unigene cluster: Hs.508113

    Expression variation in blood from EXPOLDB for OLFM4

    UniProtKB/Swiss-Prot: OLFM4_HUMAN, Q6UX06
    Tissue specificity: Expressed during myeloid lineage development. Strongly expressed in the
    prostate, small intestine and colon and moderately expressed in the bone marrow and stomach.
    Highly expressed in pancreatic cancer tissues and shows an elevated expression level during the
    early S phase of the cell cycle. Also expressed at high levels in stomach cancer and colon cancer
    tissues

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for OLFM4 gene from 5/9 species (see all 9 )
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    OLFM41   -- olfactomedin 4 85.54(n)
    82.7(a)
    485470  XM_542589.2  XP_542589.2 
    chimpanzee
    (Pan troglodytes)
    OLFM41   -- olfactomedin 4 99.48(n)
    99.8(a)
    467367  XM_522764.2  XP_522764.1 
    cow
    (Bos taurus)
    OLFM41   -- olfactomedin 4 79.63(n)
    74.75(a)
    515082  XM_593034.3  XP_593034.3 
    rat
    (Rattus norvegicus)
    Olfm41   -- olfactomedin 4 75.38(n)
    71.06(a)
    290409  XM_224413.4  XP_224413.4 
    mouse
    (Mus musculus)
    Olfm41, 5 145
    olfactomedin 41, 5 76.55(n)1
    72.58(a)1
    3809241  NM_001030294.11  NP_001025465.11 
     AK1335365  BC1411275  (see all 7)
    About this table        Species with no ortholog for OLFM4

    ENSEMBL Gene Tree for OLFM4
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for OLFM4 gene
    GLDN2  OLFML32  OLFML12  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/157 NCBI SNPs in OLFM4 are shown (see all 157 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 113)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 13 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    rs14192211,2
    A,C,F,H,O52523911(-) AAATTA/GCATGC 1 -- ut31 ese39Minor allele frequency- G:0.09MN EU EA WA 1022
    rs95363421,2
    A,C,F,H52500093(+) CTGAGT/CAAGAG 1 -- ng513Minor allele frequency- C:0.38EU EA 300
    rs125521,2
    A,C,F,H52523782(-) AAAGTC/TCATAA 1 -- ut319Minor allele frequency- T:0.43MN NA EU EA WA 896
    rs95363431,2
    A,C,F,H52500123(+) AGTTTC/TATGAA 1 -- ng5111Minor allele frequency- T:0.24EU EA WA NA 970
    rs95631301,2
    A,C,F,H52499970(+) AGAGCA/TGGGTC 1 -- ng514Minor allele frequency- T:0.37EU EA WA 394
    rs175520471,2
    C,F,H52500463(+) TCAACG/AACAAC 1 -- ng5116Minor allele frequency- A:0.23NA EA EU WA 1470
    rs22982291,2
    C,F,H52500971(+) GGACAA/GGATGA 1 -- ng51 ese39Minor allele frequency- G:0.24EA EU WA 2336
    rs95687931,2
    C,F,H52500033(+) ACAAAG/AGTGTG 1 -- ng514Minor allele frequency- A:0.11EU EA WA 416
    rs170538491,2
    C,F,H52523625(+) TCATAA/GCTTCC 1 -- ut317Minor allele frequency- G:0.16NA EU EA WA 548
    --
    rs65617031,2
    A,C52499743(+) actacA/Tgccgc 1 -- ng510--------
    About this table

    HapMap Linkage Disequilibrium images for OLFM4 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

      --

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/16 PubMed articles for OLFM4 gene (see all 16 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 10562 HGNC: 17190 AceView: OLFM4 Ensembl:ENSG00000102837 euGenes: HUgn10562
    ECgene: OLFM4 H-InvDB: OLFM4
    (According to HUGE)
    About This Section

    HUGE: KIAA4294
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
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      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for OLFM4:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



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     Predesigned and custom siRNAs for OLFM4 Antibodies for OLFM4
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for OLFM4 Browse small molecules at Sigma-Aldrich
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