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OLFM2 Gene

protein-coding GIFtS: 53

GC19M009825

olfactomedin 2
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc , and/or ⁷Ensembl, ⁸miRBase)
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Aliases
NOE2 ^¹, ², ³
NOELIN2 ^²
NOELIN2_V1 ^²
OlfC ^¹, ²
Olfactomedin-2 ^³

Descriptions
neuronal olfactomedin related ER localized protein 2 ^²
noelin 2 ^²
olfactomedin 2 ^²

External Ids
HGNC: 17189¹
Entrez Gene: 93145²
UniProtKB: O95897³
Ensembl: ENSG00000105088⁷

Search outside databases for aliases for OLFM2 gene

Previous GC identifers: GC19M010085 GC19M010187 GC19M009809

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Gene Wiki entry for OLFM2

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
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Genomic View:

UCSC Golden Path with GeneCards custom track

Transcription factor binding sites upstream to the OLFM2 gene

Entrez Gene cytogenetic band: 19p13.2 Ensembl cytogenetic band: 19p13.2 HGNC cytogenetic band: 19p13.2

OLFM2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 19 GeneLoc Exon Structure

GeneLoc location for GC19M009825: (about GC identifiers)

Start:	9,825,394 bp from pter
End:	9,908,228 bp from pter
Size:	82,835 bases
Orientation:	minus strand

RefSeq DNA sequence:

NC_000019.8 NT_011295.10

(According to ¹UniProtKB, and/or Ensembl, Phosphorylation sites according to ²Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: NOE2_HUMAN, O95897 (See protein sequence)

Recommended Name: Noelin-2 precursor

Size: 454 amino acids; 51386 Da

Subcellular location: Secreted (Probable)

Secondary accessions: Q6IMJ3 Q96FC2

REFSEQ proteins: NP_477512.1

ENSEMBL proteins:

ENSP00000264833

Human Recombinant Proteins

	Browse Drug Discovery Central at Invitrogen for human recombinant proteins
	Browse Purified and Recombinant Proteins at Millipore
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	Browse R&D Systems for human recombinant proteins
	Browse recombinant and purified proteins available from Enzo Life Sciences
	Browse Abcam for Recombinant Proteins
	Human Recombinant Proteins from Abnova (OLFM2)

OriGene Purified Recombinant Human Protein: OLFM2

1 Gene Ontology (GO) cellular component term (links to tree view):

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005576	extracellular region	IEA	--

About this table

Antibodies for OLFM2:

	Browse Antibodies Central at Invitrogen
	Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
	Browse Antibodies at Sigma-Aldrich
	R&D Systems Antibodies for OLFM2 (Noelin-2)
	Search for antibodies at Abcam and the World's Antibody Gateway (free search engine of over 150 antibody companies)
	Browse Abnova for Monoclonal and Polyclonal Antibodies
	Search Novus for antibodies for OLFM2

Assays for OLFM2:

	Browse Invitrogen for biochemical assays
	Browse Kits and Assays available from Millipore
	Browse R&D Systems for biochemical assays
	Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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Graphical View of Domain Structure for InterPro Entry O95897

ProtoNet protein and cluster: O95897

1 Blocks protein family: IPB003112 Olfactomedin-like

UniProtKB/Swiss-Prot: NOE2_HUMAN, O95897

Similarity: Contains 1 olfactomedin-like domain

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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Inhib. RNA:		Invitrogen RNAi Products for gene knock-down (OLFM2)
		Browse for Gene Knock-down Tools from Millipore
		Abnova Chimera RNAi Products for Gene knock-down (OLFM2)

OriGene 29mer shRNA kit in GFP-retroviral vector: NM_058164

Applied Biosystems Silencer® siRNAs for OLFM2

Sigma-Aldrich siRNA for OLFM2

Sigma-Aldrich shRNA for OLFM2

Explore Sigma-Aldrich super-pooled esiRNAs

Clones:		Invitrogen Clones for OLFM2
		Browse Clones for the Expression of Recombinant Proteins Available from Millipore

OriGene GFP tagged cDNA clone in CMV expression vector: NM_058164
Myc/DDK tagged cDNA clone in CMV expression vector: NM_058164
untagged cDNA clones in CMV expression vector (see all 2): NM_058164

Primers:

Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers

OriGene genome-wide validated SYBR primer pairs: NM_058164

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to ¹UniProtKB, ²MINT, and/or ³STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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5/9 Interacting proteins for OLFM2 (O95897²) via UniProtKB, MINT, and/or STRING (see all 9 )
Interactant Interaction Details
GeneCard External ID(s)
EIF1 P41567² MINT-63323
FANCF Q9NPI8² MINT-63328
GNB5 O14775² MINT-63321
PKM2 P14618² MINT-63322
ROBO2 Q9HCK4² MINT-63327
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(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences

Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for OLFM2

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib. RNA:		Invitrogen RNAi Products for gene knock-down (OLFM2)
		Browse for Gene Knock-down Tools from Millipore
		Abnova Chimera RNAi Products for Gene knock-down (OLFM2)

OriGene 29mer shRNA kit in GFP-retroviral vector: NM_058164

Sigma-Aldrich siRNA for OLFM2

Sigma-Aldrich shRNA for OLFM2

Explore Sigma-Aldrich super-pooled esiRNAs

Applied Biosystems Silencer® siRNAs:

NM_058164

REFSEQ mRNAs for OLFM2 gene:

NM_058164.2

Applied Biosystems TaqMan ® Gene Expression Assays:

NM_058164

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_058164
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_058164
                                 untagged cDNA clones in CMV expression vector (see all 2): NM_058164

Additional cDNA sequence:

AF131839.1 BC011361.1 CR592038.1 CR592581.1 CR594920.1 CR597123.1 CR598222.1 CR604836.1

CR606369.1 CR612883.1

4 DOTS entries:

DT.437658 DT.95071875 DT.91707321 DT.100837294

24/114 AceView cDNA sequences (see all 114 ):

BM479224 C00947 CR598222 CR597123 AW962045 CR612883 CR594920 CR592038

T04866 AI834308 CR592581 R40423 CR604836 BM554133 AI902497 AW273242

CR606369 AA324877 BG761933 BE252295 BQ428091 BM926586 BI834112 BQ067224

highest scoring ESTs for OLFM2:

AF131839 BC011361 BF526894 BG761933 BI755781 BI919227 BM016762 BM914092 AA126059 AA126164

Unigene Cluster for OLFM2:

Olfactomedin 2

Hs.169743 [show with all ESTs]

Unigene Representative Sequence: CR612883

GeneLoc Exon Structure

4 Alternative Splicing Database (ASD) splice patterns (SP) for OLFM2

ExUns:	1	^	2	^	3	^	4	^	5a	·	5b	·	5c	^	6	^	7a	·	7b	·	7c
SP1:					-								-
SP2:													-
SP3:											-		-
SP4:					-

About this scheme

ECgene alternative splicing isoforms for OLFM2

1 Ensembl transcript including schematic representation:

ENST00000264833

(Experimental results according to ¹GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to ²GeneAnnot , ³GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

OLFM2 expression in normal and diseased human tissues

Applied Biosystems TaqMan ® Gene Expression Assays for OLFM2

¹ / ² / ³

3 probe-sets matching OLFM2 gene

Affymetrix probe-set	Array	# genes	Sensitivity	Specificity	Correlation	Length	Gb_Accession	Consensus	Uniqueness	Score	Rank
Affymetrix probe-set	Array	GeneAnnot data			GeneNote data		GeneTide data
47970_at²^{, 3}	U95-B	1	1.00	1.00	1.00	1.00	AA126059	0.60	1.00	0.82	1
223601_at²^{, 3}	U133-B	1	1.00	1.00	--	--	AF131839	0.80	1.00	0.91	1
223601_at²	U133Plus2	1	1.00	1.00	--	--	--	--	--	--	--

About this table

Data from

(Publications) and GNF BioGPS
About these images
About these images

CGAP SAGE TAG: GATCTGTTTC

SOURCE GeneReport for Unigene cluster: Hs.169743

(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD and/or ⁵MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
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Orthologs for OLFM2 gene from 5/6 species (see all 6 )

Organism	Gene	Locus	Description	Human Similarity	NCBI accessions
dog (Canis familiaris)	OLFM2¹	--	olfactomedin 2	93.16(n) 98.14(a)	484962 XM_542079.2 XP_542079.2
cow (Bos taurus)	OLFM2¹	--	olfactomedin 2	92.32(n) 98.39(a)	507252 XM_583828.3 XP_583828.3
rat (Rattus norvegicus)	Olfm2¹	--	olfactomedin 2	88.38(n) 97.92(a)	313783 NM_001015017.1 NP_001015017.1
mouse (Mus musculus)	Olfm2^{1, 5}	9⁵	olfactomedin 2^{1, 5}	88.2(n)¹ 97.47(a)¹	244723¹ NM_173777.2¹ NP_776138.1¹ AK037199⁵ BC115981⁵ (see all 7)
zebrafish (Danio rerio)	Dr.10562²	--	Danio rerio mRNA similar to olfactomedin 2 (cDNA clone more	76.73(n)	BC044164.1

About this table Species with no ortholog for OLFM2

ENSEMBL Gene Tree for OLFM2

(Paralogs according to ¹HomoloGene
and ²Ensembl, Pseudogenes according to ³Pseudogene.org)
About This Section

Paralogs for OLFM2 gene

MYOC² OLFM3² OLFM1²

(According to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section

10/530 NCBI SNPs in OLFM2 are shown (see all 530 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay) (see all 101)

AB	SNP ID	Valid	Chr 19 pos	Sequence	Recs	AA Chg	Type	More	Recs	Pop	Total sample	More
AB	Genomic Data				Transcription Data				Allele Frequencies
				--	--	--		--	--			--
	rs9304977^1,2	A,C,F,H	9825372(+)	`CATTGG/TACACA`	1	--	ng5¹		4	EU EA WA	416
	rs2303100^1,2	C,F,H	9829434(-)	`TGCGCG/AGCTCC`	1	Q/R	mis¹ ese³		13	EA NA EU WA	2552
	rs11556087^1,2	C,F	9829139(-)	`GATGAC/TGGAAC`	1	T/M	mis¹		5	NA EU EA WA	492
--	rs11556088^1,2	C,F	9825946(-)	`GAGCGC/TGCCCT`	1	R/R	syn¹ ese³		1	NA	70
	rs6511267^1,2	A,C,F,H	9892745(+)	`ataccT/Catatg`	1	--	int¹		11	EU EA WA NA	968
	rs8104943^1,2	A,C,F,H	9888470(+)	`AACTCC/TTCTCA`	1	--	int¹		8	EU EA WA NA	782
	rs1862471^1,2	A,C,F,H	9861322(-)	`TCACAG/CACACC`	1	--	int¹		8	NA EA MN EU WA	878
	rs8099982^1,2	A,C,F,H	9853038(+)	`GCCTGG/ACCGGG`	1	--	int¹		4	EU EA WA	418
	rs1363755^1,2	C,F,H,O	9843413(-)	`CCACCA/CGTGAG`	1	--	int¹		7	EU EA WA NA	558
	rs6511284^1,2	A,C,F,H	9906125(+)	`TTCTGG/TCAGCA`	1	--	int¹		4	EU EA WA	416

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HapMap Linkage Disequilibrium images for OLFM2 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

Human Gene Mutation Database: OLFM2
Human Genome Epidemiology Navigator: OLFM2 (1 document)

(Possibly Related Articles in Doctor's Guide)
About This Section

(in PubMed. Associations of this gene to articles via ¹Novoseek, ²HGNC, ³Entrez Gene, ⁴UniProtKB/Swiss-Prot, ⁵UniProtKB/TrEMBL, ⁶GAD, and/or ⁷PharmGKB)
About This Section

10/12 PubMed articles for OLFM2 gene (see all 12 ):

Bioinformatic approaches for identification and characterization of olfactomedin related genes with a potential role in pathogenesis of ocular disorders. (PubMed id 15123989)^{1, 3, 4} Mukhopadhyay A.... Ray K. (2004)
The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)^{3, 4} Gerhard D.S....Malek J. (2004)
The DNA sequence and biology of human chromosome 19. (PubMed id 15057824)^{3, 4} Grimwood J.... Lucas S.M. (2004)
SNPs and interaction analyses of noelin 2, myocilin, and optineurin genes in Japanese patients with open-angle glaucoma. (PubMed id 17122126)^{1, 3} Funayama T....Shimada N. (2006)
The consensus coding sequences of human breast and colorectal cancers. (PubMed id 16959974)⁴ Sjoeblom T.... Velculescu V.E. (2006)
A human protein-protein interaction network: a resource for annotating the proteome. (PubMed id 16169070)³ Stelzl U....Wanker E.E. (2005)
Gene expression profile of the human trabecular meshwork: NEIBank sequence tag analysis. (PubMed id 12766061)³ Tomarev S.I....Malyukova I. (2003)
Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)³ Strausberg R.L....Marra M.A. (2002)
Hepatitis C virus core protein interacts with 14-3-3 protein and activates the kinase Raf-1. (PubMed id )⁴ Aoki H.... Hino O. (2000)
Large-scale concatenation cDNA sequencing. (PubMed id 9110174)³ Yu W....Gibbs R.A. (1997)

(in PubMed, OMIM, and NCBI Bookshelf)
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		AND	OR
Aliases
Free Text

Query String		PubMed OMIM NCBI Bookshelf
	(Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
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Entrez Gene: 93145	HGNC: 17189	AceView: OLFM2	Ensembl:ENSG00000105088	euGenes: HUgn93145
ECgene: OLFM2	H-InvDB: OLFM2

(According to HUGE)
About This Section

(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

Licensable Technologies for OLFM2
(Available from WIS Yeda, Salk, Tufts)

About This Section

(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
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Products for OLFM2:

TaqMan ® Gene Expression Assays

TaqMan ® Genotyping Assays

Free SNP selection tool

Invitrogen iPath Pathways	Invitrogen BLOCK-iT™ RNAi
Invitrogen Antibodies	Invitrogen Assays
Invitrogen Clones	Invitrogen Q-PCR Products
Invitrogen Human Recombinant Kinases	Invitrogen Custom Antibody and Peptide Service
Invitrogen Proteins / Assays / Screening Services	Search Invitrogen catalog for OLFM2-related products

	Millipore Custom Antibody & Bulk Services
	Millipore Preclinical / Clinical Development Services
	Millipore Immunoassay Services
	Millipore Target Screening & Profiling Services

	Predesigned and custom siRNAs for OLFM2		Browse antibodies at Sigma-Aldrich
	Explore super-pooled esiRNAs		Browse proteins at Sigma-Aldrich
	Lentivirus-delivered shRNAs for OLFM2		Browse small molecules at Sigma-Aldrich
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	Multiplex/Array Assay Kits & Reagents		ELISpot Kits & Development Modules
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OLFM2 Gene

protein-coding GIFtS: 53

Aliases &
Descriptions
for OLFM2

Summaries
for OLFM2

Genomic Location
for OLFM2

Proteins
for OLFM2

Protein Domains/
Families
for OLFM2

Gene Function
for OLFM2

Pathways & Interactions
for OLFM2

Drugs & Compounds
for OLFM2

Transcripts
for OLFM2

Expression
for OLFM2

Orthologs
for OLFM2

Paralogs
for OLFM2

SNPs/Variants
for OLFM2

Disorders & Mutations
for OLFM2

Medical News
for OLFM2

Publications
for OLFM2

Search for OLFM2

Genome Databases showing OLFM2

Other Databases showing OLFM2

Specialized Databases showing OLFM2

Licensable Technologies
for OLFM2

Services
for OLFM2

Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

OLFM2 Gene

protein-coding GIFtS: 53

Aliases & Descriptionsfor OLFM2

Summariesfor OLFM2

Genomic Locationfor OLFM2

Proteinsfor OLFM2

Protein Domains/Familiesfor OLFM2

Gene Functionfor OLFM2

Pathways & Interactionsfor OLFM2

Drugs & Compoundsfor OLFM2

Transcriptsfor OLFM2

Expression for OLFM2

Orthologsfor OLFM2

Paralogsfor OLFM2

SNPs/Variantsfor OLFM2

Disorders & Mutationsfor OLFM2

Medical Newsfor OLFM2

Publicationsfor OLFM2

Search for OLFM2

Genome Databases showing OLFM2

Other Databases showing OLFM2

Specialized Databases showing OLFM2

Licensable Technologiesfor OLFM2

Servicesfor OLFM2

Gene Index: 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

Aliases &
Descriptions
for OLFM2

Summaries
for OLFM2

Genomic Location
for OLFM2

Proteins
for OLFM2

Protein Domains/
Families
for OLFM2

Gene Function
for OLFM2

Pathways & Interactions
for OLFM2

Drugs & Compounds
for OLFM2

Transcripts
for OLFM2

Expression
for OLFM2

Orthologs
for OLFM2

Paralogs
for OLFM2

SNPs/Variants
for OLFM2

Disorders & Mutations
for OLFM2

Medical News
for OLFM2

Publications
for OLFM2

Licensable Technologies
for OLFM2

Services
for OLFM2