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OLAH Gene

protein-coding   GIFtS: 46

GC10P015126
oleoyl-ACP hydrolase
(Previous name: thioesterase domain containing 1 )
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
(Previous symbol: THEDC1)
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
AURA1 2
EC 3.1.2.14 3
FLJ11106 1, 2
MGC51852 2
OTTHUMP00000019191 2
OTTHUMP00000019192 2
SAST 1, 2
THEDC1 2, 3
Descriptions
Thioesterase II 3
Thioesterase domain-containing protein 1 3
augmented in rheumatoid arthritis 1 2
oleoyl-ACP hydrolase 2, 3
thioesterase domain containing 1 1, 2
External Ids
HGNC: 256251
Entrez Gene: 553012
UniProtKB: Q9NV233
Ensembl: ENSG000001524637
Search outside databases for aliases for OLAH gene

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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UniProtKB/Swiss-Prot: SAST_HUMAN, Q9NV23
Function: In fatty acid biosynthesis chain termination and release of the free fatty acid product
is achieved by hydrolysis of the thio ester by a thioesterase I, a component of the fatty acid
synthetase complex. The chain length of the released fatty acid is usually C16. However, in the
mammary glands of non-ruminant mammals, and in the uropygial gland of certain waterfowl there
exists a second thioesterase which releases medium-chain length fatty acids (C8 to C2) (By
similarity)

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the OLAH gene  

Entrez Gene cytogenetic band: 10p13   Ensembl cytogenetic band:  10p13   HGNC cytogenetic band: 10p13

OLAH Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 10         GeneLoc Exon Structure

GeneLoc location for GC10P015126:     (about GC identifiers)

Start:
15,125,932 bp from pter
End:
15,155,857 bp from pter
Size:
29,926 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000010.9  NT_077569.2  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: SAST_HUMAN, Q9NV23 (See protein sequence)
Recommended Name: S-acyl fatty acid synthase thioesterase, medium chain  
Size: 265 amino acids; 29931 Da

REFSEQ proteins (2 alternative transcripts): 
NP_001034791.1  NP_060794.1  


ENSEMBL proteins: 
ENSP00000367462 ENSP00000367470 ENSP00000367473 


Human Recombinant Proteins 
Browse Drug Discovery Central at Invitrogen for human recombinant proteins
Browse Purified and Recombinant Proteins at Millipore
Browse Human Recombinant Proteins at Sigma-Aldrich  
Browse R&D Systems for human recombinant proteins
Browse recombinant and purified proteins available from Enzo Life Sciences
Browse Abcam for Recombinant Proteins
Browse Abnova for Human Recombinant Proteins
              OriGene Purified Recombinant Human Protein: OLAH 


Antibodies for OLAH: 
Browse Antibodies Central at Invitrogen
Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
Browse Antibodies at Sigma-Aldrich
Browse R&D Systems for Antibodies
Search for antibodies at Abcam and the World's Antibody Gateway (free search engine of over 150 antibody companies)
Browse Abnova for Monoclonal and Polyclonal Antibodies
Search Novus for antibodies for OLAH

Assays for OLAH: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

2 InterPro domains/families:
 IPR012223 TEII
 IPR001031 Thioesterase


   GeneDecks  OLAH for the domains selected above  
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Graphical View of Domain Structure for InterPro Entry Q9NV23

ProtoNet protein and cluster: Q9NV23

1 Blocks protein family: IPB001031 Thioesterase

UniProtKB/Swiss-Prot: SAST_HUMAN, Q9NV23
Similarity: Belongs to the thioesterase family

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (OLAH)
Browse for Gene Knock-down Tools from Millipore
Browse Abnova for Chimera RNAi Products
               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_018324

              Applied Biosystems Silencer® siRNAs for OLAH

              Sigma-Aldrich siRNA for OLAH  
                     Sigma-Aldrich shRNA for OLAH  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Browse Clone Ranger at Invitrogen for clones
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
               OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_001039702
                                 Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_001039702
                                 untagged cDNA clones in CMV expression vector (see all 3): NM_001039702 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_018324

UniProtKB/Swiss-Prot: SAST_HUMAN, Q9NV23
Function: In fatty acid biosynthesis chain termination and release of the free fatty acid product
is achieved by hydrolysis of the thio ester by a thioesterase I, a component of the fatty acid
synthetase complex. The chain length of the released fatty acid is usually C16. However, in the
mammary glands of non-ruminant mammals, and in the uropygial gland of certain waterfowl there
exists a second thioesterase which releases medium-chain length fatty acids (C8 to C2) (By
similarity)
Catalytic activity: Oleoyl-[acyl-carrier-protein] + H(2)O = [acyl-carrier-protein] + oleate
Enzyme Number (IUBMB): EC 3.1.2.14 

2 Gene Ontology (GO) molecular function terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0004320 oleoyl-[acyl-carrier-protein] hydrolase activity IEA--
GO:0016788 hydrolase activity, acting on ester bonds IEA--
About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section


1 Sigma-Aldrich "Your Favorite Gene" Pathway for  OLAH  (Your Favorite Gene powered by Ingenuity) 
 Fatty Acid Biosynthesis

   GeneDecks  OLAH for the pathways selected above  
About GeneDecksing

1 Kegg Pathway  (Kegg details for OLAH):
 hsa00061 Fatty acid biosynthesis

   GeneDecks  OLAH for the pathways selected above  
About GeneDecksing

5/7 Interacting proteins for OLAH (ENSP000003674623) via UniProtKB, MINT, and/or STRING (see all 7 )
InteractantInteraction Details
GeneCardExternal ID(s)
OXSMENSP000002807013STRING (score=.899)
ACSL1ENSP000002814553STRING (score=.8)
ACSL3ENSP000003500123STRING (score=.8)
ACSL4ENSP000003397873STRING (score=.8)
ACSL5ENSP000003484293STRING (score=.8)
About this table

1 Gene Ontology (GO) biological process term (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0006633 fatty acid biosynthetic process IEA--
About this table
(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences
Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for OLAH

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (OLAH)
Browse for Gene Knock-down Tools from Millipore
Browse Abnova for Chimera RNAi Products
               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_018324

              Sigma-Aldrich siRNA for OLAH  
                     Sigma-Aldrich shRNA for OLAH  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_001039702  NM_018324  

REFSEQ mRNAs for OLAH gene (2 alternative transcripts): 

NM_001039702.2   NM_018324.2   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_001039702  NM_018324  

               OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_001039702
                                 Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_001039702
                                 untagged cDNA clones in CMV expression vector (see all 3): NM_001039702 

Additional cDNA sequence: 

AK001844.1 AK001968.1 BC050372.1 

8 DOTS entries:

DT.91724773  DT.310766  DT.100751709  DT.75135089  DT.100751708  DT.121258176  DT.91724767  DT.95370676 

24/47 AceView cDNA sequences (see all 47 ):

AA431387 AI151217 BX118351 AA470066 BQ013235 BM554164 BC050372 AI125816 
AK001844 BE782959 AU137095 BG475148 BU167548 AI208288 AI183402 AA758593 
BF125008 AK001968 AA432379 BE781387 AI125696 NM_018324 CB991118 AA694484 

highest scoring ESTs for OLAH:

AI125696 AA470066 AA694484 AA758593 AA993723 AI025682 AI125816 AI151217 AI208288 AI219027 

Unigene Cluster for OLAH:

Oleoyl-ACP hydrolase
Hs.24309  [show with all ESTs]
Unigene Representative Sequence: NM_018324


GeneLoc Exon Structure

4 Alternative Splicing Database (ASD) splice patterns (SP) for OLAH

ExUns: 1a · 1b ^ 2a · 2b ^ 3 ^ 4 ^ 5 ^ 6 ^ 7 ^ 8a · 8b ^ 9 ^ 10
SP1:                    -                 -                 -                     
SP2:                    -                                                         
SP3:                                      -                                       
SP4:                                                                              

About this scheme

ECgene alternative splicing isoforms for OLAH

3 Ensembl transcripts including schematic representations:
ENST00000378217  ENST00000378225  ENST00000378228  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

OLAH expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for OLAH

1 / 2 / 3

7 probe-sets matching OLAH gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

43541_at2, 3 U95-B 1 1.00 1.00 1.00 1.00 AI125696 1.00 1.00 1.00 1

233126_s_at2, 3 U133-B 1 1.00 1.00 -- -- AK001844 0.80 1.00 0.91 1

222945_x_at2, 3 U133-B 1 1.00 1.00 -- -- AI125696 1.00 1.00 1.00 1

219975_x_at2, 3 U133-A 8 1.00 0.86 -- -- NM_018324 0.60 1.00 0.82 1

233126_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

222945_x_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

219975_x_at2 U133Plus2 8 1.00 0.86 -- -- -- -- -- -- --
About this table
Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: ATTAGTAAAT

SOURCE GeneReport for Unigene cluster: Hs.24309
(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for OLAH gene from 5 species
Organism Gene Locus Description Human
Similarity
NCBI accessions
cow
(Bos taurus)
OLAH1   -- oleoyl-ACP hydrolase 73.23(n)
57.43(a)
617347  XM_869588.2  XP_874681.2 
rat
(Rattus norvegicus)
Olah1   -- oleoyl-ACP hydrolase 70.47(n)
56.27(a)
64669  NM_022705.1  NP_073196.1 
mouse
(Mus musculus)
Olah1, 5 25
oleoyl-ACP hydrolase1, 5 72.7(n)1
59.25(a)1
990351  NM_145921.11  NP_666033.11 
 AI4148105  AI6073005  (see all 10)
chicken
(Gallus gallus)
OLAH1   -- oleoyl-ACP hydrolase 59.67(n)
44.03(a)
420532  XM_418634.2  XP_418634.1 
zebrafish
(Danio rerio)
zgc:1369261   -- zgc:136926 51.47(n)
41.2(a)
568096  NM_001039932.1  NP_001035021.1 
About this table        Species with no ortholog for OLAH

ENSEMBL Gene Tree for OLAH
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

  --
(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section


10/204 NCBI SNPs in OLAH are shown (see all 204 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 70)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr 10 posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
------------
rs47481401,2
A,C,F,H15155725(+) TCTCAG/CTTGAT 2 -- ut31 ese316Minor allele frequency- C:0.49EU EA WA NS NA 1086
--
rs127678531,2
C,F,H15138903(+) cctacC/Tcatgt 2 P/S mis1 int1 ese34Minor allele frequency- T:0.06EU EA WA 406
rs37637841,2
C,F,H15124496(+) TGTCAC/TGGAGA 2 -- ng51 tfbs312Minor allele frequency- N:0.01EU EA WA NA 1052
rs37637851,2
C,F,H15124574(+) CATTTT/CAGAAT 2 -- ng514Minor allele frequency- C:0.21EU EA WA 392
rs70937761,2
C,F,H15155985(+) AAGTCC/GTCAGA 2 -- ng318Minor allele frequency- G:0.14EU EA WA 814
rs70973091,2
A,C,F15156110(+) AGAGTG/CTTGCC 2 -- ng313Minor allele frequency- C:0.48NA 142
rs112594581,2
C,F,H15155706(+) TTTTAT/CCTTCC 2 -- ut314Minor allele frequency- C:0.12EU EA WA 410
--
rs70813831,2
A,C15155596(+) GGAGGC/TGGAGC 2 -- ut31 ese30--------
--
rs79064681,2
A,C15156286(+) acaccA/Tgtaat 1 -- ng310--------
--
rs125720321,2
A,C15125108(+) GATCCA/GCCCGC 2 -- ng510--------
About this table

HapMap Linkage Disequilibrium images for OLAH (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

  --

(Possibly Related Articles in Doctor's Guide)
About This Section

  --

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
About This Section

4 PubMed articles for OLAH gene:
(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)
(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 55301 HGNC: 25625 AceView: THEDC1 Ensembl:ENSG00000152463 euGenes: HUgn55301
ECgene: OLAH H-InvDB: OLAH
(According to HUGE)
About This Section

  --
(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

  --
(Available from WIS Yeda, Salk, Tufts)
About This Section

  --
(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for OLAH:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



  Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
  Invitrogen Antibodies  Invitrogen Assays
  Invitrogen Clones  Invitrogen Q-PCR Products
  Invitrogen Human Recombinant Kinases  Invitrogen Custom Antibody and Peptide Service
  Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for OLAH-related products

 Millipore Custom Antibody & Bulk Services
 Millipore Preclinical / Clinical Development Services
 Millipore Immunoassay Services
 Millipore Target Screening & Profiling Services


 Predesigned and custom siRNAs for OLAH Browse antibodies at Sigma-Aldrich
 Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
 Lentivirus-delivered shRNAs for OLAH Browse small molecules at Sigma-Aldrich
 "Your Favorite Gene" Pathwaysfeedback


  
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 Purified proteins from HEK293T cells
 Western blot validated antibodies
 shRNA in GFP-retroviral vector
 Search Tocris compounds for OLAH




 Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
 Search Novus for antibodies for OLAH

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