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OLA1 Gene

protein-coding   GIFtS: 50

GC02M174646
Obg-like ATPase 1
(Previous name: GTP-binding protein 9 (putative) )
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
(Previous symbol: GTPBP9)
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
DKFZp313H1942 2
EC 3.6.3.- 3
GTPBP9 2, 3
OTTHUMP00000163173 2
PTD004 1, 2
Descriptions
GTP-binding protein 9 3
GTP-binding protein 9 (putative) 1, 2
GTP-binding protein PTD004 2
Obg-like ATPase 1 2
homologous yeast-44.2 protein 2
External Ids
HGNC: 288331
Entrez Gene: 297892
UniProtKB: Q9NTK53
Ensembl: ENSG000001384307
Search outside databases for aliases for OLA1 gene

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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UniProtKB/Swiss-Prot: OLA1_HUMAN, Q9NTK5
Function: Hydrolyzes ATP, and can also hydrolyze GTP with lower efficiency. Has lower affinity for
GTP

Gene Wiki entry for OLA1

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 2q31.1   Ensembl cytogenetic band:  2q31.1   HGNC cytogenetic band: 2q31.1

OLA1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 2         GeneLoc Exon Structure

GeneLoc location for GC02M174646:     (about GC identifiers)

Start:
174,645,421 bp from pter
End:
174,821,611 bp from pter
Size:
176,191 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000002.10  NT_005403.16  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: OLA1_HUMAN, Q9NTK5 (See protein sequence)
Recommended Name: Obg-like ATPase 1  
Size: 396 amino acids; 44744 Da
Subunit: Monomer
PDB structures from and Proteopedia :
2OHF (3D)    
Secondary accessions: Q5BJD7 Q8NCI8 Q96CU1 Q96SV2 Q9P1D3 Q9UNY9 Q9Y6G4
Alternative splicing: 3 isoforms:  Q9NTK5-1   Q9NTK5-2   Q9NTK5-3   

REFSEQ proteins (2 alternative transcripts): 
NP_001011708.1  NP_037473.3  


ENSEMBL proteins: 
ENSP00000340167 ENSP00000386350 ENSP00000284719 ENSP00000376343 


Human Recombinant Proteins 
Browse Drug Discovery Central at Invitrogen for human recombinant proteins
Browse Purified and Recombinant Proteins at Millipore
Browse Human Recombinant Proteins at Sigma-Aldrich  
Browse R&D Systems for human recombinant proteins
Browse recombinant and purified proteins available from Enzo Life Sciences
Recombinant Proteins from Abcam (GTPBP9)
Human Recombinant Proteins from Abnova (OLA1)
              OriGene Purified Recombinant Human Protein: OLA1 

2 Gene Ontology (GO) cellular component terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005622 intracellular IEA--
GO:0005737 cytoplasm IDA11256614
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Antibodies for OLA1: 
Browse Antibodies Central at Invitrogen
Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
Sigma-Aldrich Antibodies for OLA1
Browse R&D Systems for Antibodies
Antibodies from Abcam (GTPBP9), each with their AbpromiseSM
Browse Abnova for Monoclonal and Polyclonal Antibodies
Search Novus for antibodies for OLA1

Assays for OLA1: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

5 InterPro domains/families:
 IPR002917 MMR_HSR1_GTP_bd
 IPR012675 b-grasp_ferredoxin-like
 IPR013029 DUF933
 IPR006073 GTP1_OBG
 IPR004396 CHP00092


   GeneDecks  OLA1 for the domains selected above  
About GeneDecksing

Graphical View of Domain Structure for InterPro Entry Q9NTK5

ProtoNet protein and cluster: Q9NTK5

3 Blocks protein families:
IPB002917 GTP-binding protein
IPB006073 GTP1/OBG GTP-binding protein family signature
IPB013029 Protein of unknown function DUF933


UniProtKB/Swiss-Prot: OLA1_HUMAN, Q9NTK5
Similarity: Belongs to the GTP1/OBG family

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (OLA1)
Browse for Gene Knock-down Tools from Millipore
Browse Abnova for Chimera RNAi Products
               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_001011708

              Applied Biosystems Silencer® siRNAs for OLA1

              Sigma-Aldrich siRNA for OLA1  
                     Sigma-Aldrich shRNA for OLA1  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Invitrogen Clones for OLA1
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
               OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_001011708
                                 Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_001011708
                                 untagged cDNA clones in CMV expression vector (see all 3): NM_001011708 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_013341

UniProtKB/Swiss-Prot: OLA1_HUMAN, Q9NTK5
Function: Hydrolyzes ATP, and can also hydrolyze GTP with lower efficiency. Has lower affinity for
GTP
Catalytic activity: ATP + H2O = ADP + phosphate
Enzyme Number (IUBMB): EC 3.6.3.- 

5 Gene Ontology (GO) molecular function terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0000166 nucleotide binding IEA--
GO:0005515 protein binding IPI17353931
GO:0005524 ATP binding IDA17430889
GO:0005525 GTP binding IEA--
GO:0016787 hydrolase activity IEA--
About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section



5 Interacting proteins for OLA1 (ENSP000002847193 Q9NTK51, 2) via UniProtKB, MINT, and/or STRING
InteractantInteraction Details
GeneCardExternal ID(s)
PUF60Q9UHX11EBI-766468, EBI-1053259
ASCC2Q9H1I82MINT-65484
NLRP14ENSP000002994813STRING (score=.838)
PTRH1ENSP000003342773STRING (score=.836)
--Q96K272MINT-65634
About this table

1 Gene Ontology (GO) biological process term (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0006200 ATP catabolic process IDA17430889
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(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences
Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for OLA1

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (OLA1)
Browse for Gene Knock-down Tools from Millipore
Browse Abnova for Chimera RNAi Products
               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_001011708

              Sigma-Aldrich siRNA for OLA1  
                     Sigma-Aldrich shRNA for OLA1  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_001011708  NM_013341  

REFSEQ mRNAs for OLA1 gene (2 alternative transcripts): 

NM_001011708.1   NM_013341.3   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_001011708  NM_013341  

               OriGene GFP tagged cDNA clones in CMV expression vector (see all 2): NM_001011708
                                 Myc/DDK tagged cDNA clones in CMV expression vector (see all 2): NM_001011708
                                 untagged cDNA clones in CMV expression vector (see all 3): NM_001011708 

Additional cDNA sequence: 

AF078859.1 AF078868.1 AF090946.1 AF134478.1 AK027523.1 AK074710.1 AK225112.1 AK225138.1 
AK296339.1 AL136546.1 AL833441.1 BC012842.1 BC013925.1 BC029376.1 BC068447.1 BC091522.1 
CR591506.1 CR594307.1 CR596070.1 CR603515.1 CR604617.1 CR604894.1 CR606753.1 CR606953.1 
CR607181.1 CR607795.1 CR607845.1 CR608088.1 CR608516.1 CR613657.1 CR618778.1 CR618959.1 
CR621118.1 

24/29 DOTS entries (see all 29 ):

DT.75123637  DT.100880350  DT.100692069  DT.91777312  DT.120967622  DT.100766712  DT.100880345  DT.91777295 
DT.100880343  DT.95368241  DT.100663964  DT.100880347  DT.120967581  DT.100880341  DT.100880351  DT.100880352 
DT.102834815  DT.120667025  DT.120967580  DT.120967585  DT.120967657  DT.120967666  DT.120967676  DT.91777309 

24/540 AceView cDNA sequences (see all 540 ):

AA242978 AI082010 AA494127 BG700826 BM922070 BG501274 BU153539 CB131631 
CB133682 AA307942 BQ437576 AA236854 CR596070 BG209293 AF134478 BI667004 
BM904888 BM542668 BC029376 CA419824 AA019294 AA233500 CR604617 AA608631 

Unigene Cluster for OLA1:

Obg-like ATPase 1
Hs.157351  [show with all ESTs]
Unigene Representative Sequence: NM_013341


GeneLoc Exon Structure

5/9 Alternative Splicing Database (ASD) splice patterns (SP) for OLA1 (see all 9 )

ExUns: 1a · 1b · 1c · 1d · 1e ^ 2 ^ 3 ^ 4a · 4b ^ 5a · 5b ^ 6 ^ 7 ^ 8 ^ 9 ^ 10a · 10b ^ 11a · 11b ^ 12a · 12b · 12c ^ 13a · 13b ^ 14
SP1:                                                                    -     -                                                     -                     
SP2:                                -                                   -     -                                                                           
SP3:                                -     -                                   -                                                                           
SP4:                                -     -                             -     -                                                                           
SP5:                                -     -                             -     -           -     -     -                                                   

About this scheme

ECgene alternative splicing isoforms for OLA1

4 Ensembl transcripts including schematic representations:
ENST00000344357  ENST00000409546  ENST00000284719  ENST00000392560  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

OLA1 expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for OLA1

1 / 2

6 probe-sets matching OLA1 gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

42330_at2 U95-B 1 1.00 1.00 0.94 0.72 -- -- -- -- --

65966_at2 U95-C 1 1.00 1.00 0.92 1.28 -- -- -- -- --

224525_s_at2 U133-B 1 1.00 1.00 -- -- -- -- -- -- --

219293_s_at2 U133-A 3 1.00 0.89 -- -- -- -- -- -- --

224525_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

219293_s_at2 U133Plus2 3 1.00 0.89 -- -- -- -- -- -- --
About this table
Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: TTTTACAGTA

SOURCE GeneReport for Unigene cluster: Hs.157351
(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for OLA1 gene from 5/28 species (see all 28 )
Organism Gene Locus Description Human
Similarity
NCBI accessions
dog
(Canis familiaris)
OLA11   -- Obg-like ATPase 1 96.54(n)
99.16(a)
478803  XM_845227.1  XP_850320.1 
chimpanzee
(Pan troglodytes)
OLA11   -- Obg-like ATPase 1 100(n)
100(a)
459751  XM_515918.2  XP_515918.2 
cow
(Bos taurus)
OLA11   -- Obg-like ATPase 1 96.46(n)
99.24(a)
509966  NM_001046045.1  NP_001039510.1 
rat
(Rattus norvegicus)
Ola11   -- Obg-like ATPase 1 92.93(n)
98.23(a)
296488  NM_001033927.1  NP_001029099.1 
mouse
(Mus musculus)
Ola11, 5 25
Obg-like ATPase 11, 5 93.43(n)1
99.49(a)1
670591  NM_025942.21  NP_080218.11 
 AA1204875  AI4513815  (see all 25)
About this table        Species with no ortholog for OLA1

ENSEMBL Gene Tree for OLA1
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

  --
(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section


10/851 NCBI SNPs in OLA1 are shown (see all 851 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 296)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr 2 posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
------------
rs37391521,2
A,C,F,H174645713(-) TATTAG/AAATTC 2 -- ut314Minor allele frequency- A:0.30EU EA WA 406
rs124667881,2
A,C,F,H174645097(+) ACTTGC/TCATTC 2 -- ng514Minor allele frequency- T:0.29EU EA WA 406
rs67376291,2
A,C,F,H174647414(+) CTTAAT/CTTACC 2 -- ut317Minor allele frequency- C:0.41NA EU EA WA 558
rs37391531,2
A,C,F,H174645524(-) AATGTT/CGGTTG 2 -- ut318Minor allele frequency- C:0.15EA NA EU WA 2046
rs38208981,2
A,C,F,H174821758(-) GGGAAC/TGGCCT 2 -- ng317Minor allele frequency- T:0.30EA MN EU WA 3172
rs173165001,2
F,H174647433(+) CACTTC/TGACTG 2 -- ut31 ese37Minor allele frequency- T:0.03NA EU EA WA 558
rs351451021,2
F,H174647329(+) AGAAGG/ACTAAG 2 -- ut314Minor allele frequency- A:0.37EU EA WA 420
rs75832881,2
A,C174821941(+) GGGGCA/GTCTGA 2 -- ng310--------
--
rs75836111,2
A,C174822267(+) ccctaA/Gacaaa 2 -- ng310--------
--
rs115589891,2
C174802351(-) TGATCA/CTAATG 2 P/H mis1 ut51 ese30--------
About this table

HapMap Linkage Disequilibrium images for OLA1 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 611175

(Possibly Related Articles in Doctor's Guide)
About This Section

  --

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
About This Section

10/14 PubMed articles for OLA1 gene (see all 14 ):
(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)
(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 29789 HGNC: 28833 AceView: PTD004 Ensembl:ENSG00000138430 euGenes: HUgn29789
ECgene: OLA1 H-InvDB: OLA1
(According to HUGE)
About This Section

  --
(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

  --
(Available from WIS Yeda, Salk, Tufts)
About This Section

  --
(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for OLA1:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



  Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
  Invitrogen Antibodies  Invitrogen Assays
  Invitrogen Clones  Invitrogen Q-PCR Products
  Invitrogen Human Recombinant Kinases  Invitrogen Custom Antibody and Peptide Service
  Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for OLA1-related products

 Millipore Custom Antibody & Bulk Services
 Millipore Preclinical / Clinical Development Services
 Millipore Immunoassay Services
 Millipore Target Screening & Profiling Services


 Predesigned and custom siRNAs for OLA1 Antibodies for OLA1
 Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
 Lentivirus-delivered shRNAs for OLA1 Browse small molecules at Sigma-Aldrich
 "Your Favorite Gene" Pathwaysfeedback


  
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 Recombinant Proteins (OLA1)
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 Recombinant Proteins
(GTPBP9)
 Antibodies (GTPBP9)
 Tagged/untagged cDNA clones
 Validated SYBR primer pairs
 Purified proteins from HEK293T cells
 Western blot validated antibodies
 shRNA in GFP-retroviral vector
 Search Tocris compounds for OLA1




 Search www.enzolifesicences.com for proteins, assays, substrates, inhibitors & antibodies
 Search Novus for antibodies for OLA1

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