Home
GeneCards Guide
Suite
- MalaCards
- GeneALaCart
- GeneDecks
- GeneIP
- GeneLoc
Terms of Use
- Terms of Use
- 3rd Party Notice
About Us
- GeneCards Team
- Our Publications
- Citing GeneCards
- Licensing Agreement
- Weizmann Institute
- LifeMap Sciences Inc
User Feedback
GeneCards Plus
- Premium Tools
  
  Learn more

Set Analyses:

VarElect

Advanced Search

OCLN Gene

protein-coding GIFtS: 60
GCID: GC05P068788

Occludin

Alzheimer's & Parkinson's Diseases Congress

See related diseases
at

MalaCards

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc, ⁷Ensembl, ⁸DME, ⁹miRBase, ¹⁰fRNAdb, ¹²H-InvDB, ¹³NCBI, ¹⁴NONCODE, and/or ¹⁵RNAdb)
About This Section

TryGeneCards Plus

Aliases
occludin¹ ²
BLCPMG² ⁵
Tight Junction Protein Occludin TM4 Minus¹
Tight Junction Protein Occludin²

External Ids:	HGNC: 8104¹	Entrez Gene: 100506658²	Ensembl: ENSG00000197822⁷	OMIM: 602876⁵	UniProtKB: Q16625³

Export aliases for OCLN gene to outside databases

Previous GC identifers: GC05P068940 GC05P070353 GC05P068803 GC05P068823 GC05P065744

(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

TryGeneCards Plus

Entrez Gene summary for OCLN Gene:

This gene encodes an integral membrane protein that is required for cytokine-induced regulation of the tight

junction paracellular permeability barrier. Mutations in this gene are thought to be a cause of band-like

calcification with simplified gyration and polymicrogyria (BLC-PMG), an autosomal recessive neurologic disorder

that is also known as pseudo-TORCH syndrome. Alternative splicing results in multiple transcript variants. A

related pseudogene is present 1.5 Mb downstream on the q arm of chromosome 5. (provided by RefSeq, Apr 2011)

GeneCards Summary for OCLN Gene:

OCLN (occludin) is a protein-coding gene. Diseases associated with OCLN include pseudo-torch syndrome, and torch syndrome. GO annotations related to this gene include structural molecule activity.

UniProtKB/Swiss-Prot: OCLN_HUMAN, Q16625

Function: May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability

barrier. It is able to induce adhesion when expressed in cells lacking tight junctions

Gene Wiki entry for OCLN (Occludin) Gene

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
About This Section

TryGeneCards Plus

RefSeq DNA sequence:

NC_000005.10 NT_034772.7 NC_018916.2

Regulatory elements:

Regulatory transcription factor binding sites in the OCLN gene promoter:
GR Sox5 GR-beta AP-2gamma C/EBPalpha c-Myb GR-alpha AP-2alpha
Other transcription factors

SwitchGear Promoter luciferase reporter plasmids (see all 2): OCLN promoter sequence

Search Chromatin IP Primers for OCLN

Epigenetics:

DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat OCLN

Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 5q13.1 Ensembl cytogenetic band: 5q13.2 HGNC cytogenetic band: 5q13.1

OCLN Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
OCLN gene location

GeneLoc information about chromosome 5 GeneLoc Exon Structure

GeneLoc location for GC05P068788: view genomic region (about GC identifiers)

Start:	68,788,119 bp from pter	End:	68,853,931 bp from pter
Size:	65,813 bases	Orientation:	plus strand

1 alternative location:

Chr5+,NW_003315917.2 275,189-341,052

(According to ¹UniProtKB, HORDE, ²neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Cloud-Clone Corp.,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Cloud-Clone Corp., Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, and/or Cloud-Clone Corp.)
About This Section

TryGeneCards Plus

UniProtKB/Swiss-Prot: OCLN_HUMAN, Q16625 (See protein sequence)

Recommended Name: Occludin

Size: 522 amino acids; 59144 Da

Subunit: Interacts with TJP1/ZO1 and with VAPA

3 PDB 3D structures from and Proteopedia for OCLN:

1WPA (3D)

1XAW (3D)

3G7C (3D)

Secondary accessions: B5BU70 D2DU64 D2DU65 D2IGC0 D2IGC1 E2CYV9 Q5U1V4 Q8N6K1

Alternative splicing: 7 isoforms: Q16625-1 Q16625-2 Q16625-3 Q16625-4 Q16625-5 Q16625-6 Q16625-7

Explore the universe of human proteins at neXtProt for OCLN: NX_Q16625

Explore proteomics data for OCLN at MOPED

Post-translational modifications:

Dephosphorylated by PTPRJ. The tyrosine phosphorylation on Tyr-398 and Tyr-402 reduces its ability to interact

with TJP1. Phosphorylation at Ser-490 also attenuates the interaction with TJP1¹

Ubiquitination² at Lys276, Lys283, Lys299

Modification sites at PhosphoSitePlus

See OCLN Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

REFSEQ proteins (3 alternative transcripts):

NP_001192183.1 NP_001192184.1 NP_002529.1

ENSEMBL proteins:

ENSP00000347379 ENSP00000379719 ENSP00000370143 ENSP00000445940 ENSP00000440000

Reactome Protein details: Q16625

OCLN Human Recombinant Protein Products:

	Browse Purified and Recombinant Proteins at EMD Millipore
	Browse R&D Systems for human recombinant proteins
	Browse recombinant and purified proteins available from Enzo Life Sciences
	OriGene Purified Protein for OCLN OriGene Protein Over-expression Lysate for OCLN OriGene MassSpec for OCLN OriGene Custom Protein Services for OCLN
	GenScript Custom Purified and Recombinant Proteins Services for OCLN
	Novus Biologicals OCLN Proteins Novus Biologicals OCLN Lysates
	Browse Sino Biological Recombinant Proteins Browse Sino Biological Cell Lysates
	Browse ProSpec Recombinant Proteins
	Cloud-Clone Corp. Proteins for OCLN

OCLN Antibody Products:

	EMD Millipore Mono- and Polyclonal Antibodies for the study of OCLN
	R&D Systems Antibodies for OCLN (Occludin)
	OriGene Antibodies for OCLN OriGene Custom Antibody Services for OCLN
	Novus Biologicals OCLN Antibodies
	Abcam antibodies for OCLN
	Cloud-Clone Corp. Antibodies for OCLN
	ThermoFisher Antibodies for OCLN
	LSBio Antibodies in human, mouse, rat for OCLN

OCLN Assay Products:

	Browse Kits and Assays available from EMD Millipore
	OriGene Custom Assay Services for OCLN
	Browse R&D Systems for biochemical assays
	GenScript Custom Assay Services for OCLN
	Browse Enzo Life Sciences for kits & assays
	Cloud-Clone Corp. ELISAs for OCLN Cloud-Clone Corp. CLIAs for OCLN

(According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

TryGeneCards Plus

4 InterPro protein domains:

IPR008253 Marvel

IPR002958 Occludin

IPR010844 Occludin_RNApol2_elong_fac_ELL

IPR016196 MFS_dom_general_subst_transpt

Graphical View of Domain Structure for InterPro Entry Q16625

ProtoNet protein and cluster: Q16625

3 Blocks protein domains:

IPB002958 Occludin signature

IPB008253 Marvel

IPB010844 Occludin and RNA polymerase II elongation factor ELL

UniProtKB/Swiss-Prot: OCLN_HUMAN, Q16625

Domain: The C-terminal is cytoplasmic and is important for interaction with ZO-1. Sufficient for the tight

junction localization. Involved in the regulation of the permeability barrier function of the tight junction (By

similarity). The first extracellular loop participates in an adhesive interaction

Similarity: Belongs to the ELL/occludin family

Similarity: Contains 1 MARVEL domain

OCLN for domains About GeneDecksing

(According to ¹UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or ²DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
About This Section

TryGeneCards Plus

Molecular Function:

UniProtKB/Swiss-Prot Summary: OCLN_HUMAN, Q16625

Function: May play a role in the formation and regulation of the tight junction (TJ) paracellular permeability

barrier. It is able to induce adhesion when expressed in cells lacking tight junctions

Genatlas biochemistry entry for OCLN:

occludin,60kDa,integral membrane at tight junctions of epithelial cells,highly expressed in

testis,kidney,liver,lung,brain,involved in the barrier and fence function of TJ,accessory protein in TJ strands

formation

Gene Ontology (GO): 5 molecular function terms: About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005198	structural molecule activity	IEA	--
GO:0005515	protein binding	IPI	16616143
GO:0008119	thiopurine S-methyltransferase activity	IEA	--
GO:0008757	S-adenosylmethionine-dependent methyltransferase activity	--	--
GO:0019904	protein domain specific binding	IPI	--

OCLN for ontologies About GeneDecksing

Phenotypes:
11 MGI mutant phenotypes (inferred from 4 alleles) (MGI details for Ocln):

behavior/neurological	digestive/alimentary	endocrine/exocrine gland	growth/size/body	homeostasis/metabolism
immune system	limbs/digits/tail	muscle	nervous system	reproductive system
skeleton

OCLN for phenotypes About GeneDecksing

Animal Models:
MGI mouse knock-out Ocln^tm2Sts for OCLN

inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for OCLN

inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for OCLN

genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for OCLN

genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for OCLN

miRNA Products:		miRTarBase miRNAs that target OCLN: hsa-mir-335-5p (MIRT018585), hsa-let-7b-5p (MIRT052167), hsa-let-7e-5p (MIRT051654)

		Block miRNA regulation of human, mouse, rat OCLN using miScript Target Protectors
		Selected qRT-PCR Assays for microRNAs that regulate OCLN (see all 59):
		hsa-miR-124* hsa-miR-21* hsa-miR-607 hsa-miR-429 hsa-miR-500a hsa-miR-371-5p hsa-miR-570 hsa-miR-548l

		SwitchGear 3'UTR luciferase reporter plasmid: OCLN 3' UTR sequence

Inhib. RNA Products:		OriGene RNAi products in human, mouse, rat for OCLN
		Predesigned siRNA for gene silencing in human, mouse, rat OCLN

Gene Editing
Products:

DNA2.0 Custom Protein Engineering Service for OCLN

Clone Products:		OriGene clones in human, mouse for OCLN (see all 4) OriGene ORF clones in mouse, rat for OCLN OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
		GenScript: all cDNA clones in your preferred vector (see all 10): OCLN (XM_003118545)
		Sino Biological Human cDNA Clone for OCLN
		DNA2.0 Custom Codon Optimized Gene Synthesis Service for OCLN
		Search Vector BioLabs for ready-to-use adenovirus/AAV for human, mouse, rat OCLN

Cell Line Products:		GenScript Custom overexpressing Cell Line Services for OCLN
		Browse ESI BIO Cell Lines and PureStem Progenitors for OCLN

In Situ Assay
Products:

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for OCLN

(According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
About This Section

TryGeneCards Plus

Subcellular locations from UniProtKB/Swiss-Prot

OCLN_HUMAN, Q16625: Membrane; Multi-pass membrane protein. Cell junction, tight junction

Subcellular locations from COMPARTMENTS:

Compartment	Confidence
plasma membrane	4
cytoskeleton	3
extracellular	2
peroxisome	2
endosome	1
nucleus	1

About this image

Gene Ontology (GO): Selected cellular component terms (see all 11): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005829	cytosol	IEA	--
GO:0005886	plasma membrane	TAS	--
GO:0005911	cell-cell junction	IDA	19332538
GO:0005923	tight junction	IDA	--
GO:0016020	membrane	--	--

OCLN for ontologies About GeneDecksing

(SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to ¹UniProtKB, ²MINT, ³I2D, and/or ⁴STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
About This Section

TryGeneCards Plus

SuperPaths for OCLN About (see all 8)

See pathways by source

SuperPath

Contained pathways About

Development Slit Robo signaling

Pathogenic Escherichia coli infection

0.43

Pathogenic Escherichia coli infection

0.41

Sertoli-Sertoli Cell Junction Dynamics

Sertoli-Sertoli Cell Junction Dynamics

0.38

Epithelial Tight Junctions

0.36

Integrin Pathway

Transendothelial Migration of Leukocytes

0.38

Tight junction

Tight junction

TGF-beta receptor signaling

TGF-beta receptor signaling

Pathways by source See SuperPaths
Show all pathways

3 Downloadable PowerPoint Slides of GeneGlobe Pathway Central Maps for OCLN

	Sertoli-Sertoli Cell Junction Dynamics
	Epithelial Tight Junctions
	Transendothelial Migration of Leukocytes

3 GeneGo (Thomson Reuters) Pathways for OCLN

	Cell adhesion Endothelial cell contacts by junctional mechanisms
	Cell adhesion Tight junctions
	Cell adhesion Gap junctions

2 BioSystems Pathways for OCLN

	Pathogenic Escherichia coli infection
	TGF-beta receptor signaling

1 Reactome Pathway for OCLN

Apoptotic cleavage of cell adhesion proteins

5 Kegg Pathways (Kegg details for OCLN):

	Cell adhesion molecules (CAMs)
	Tight junction
	Leukocyte transendothelial migration
	Pathogenic Escherichia coli infection
	Hepatitis C

OCLN for pathways About GeneDecksing

Pathway & Disease-focused RT² Profiler PCR Arrays including OCLN:

Endothelial Cell Biology in human mouse rat

Tight Junctions in human mouse rat

Cancer PathwayFinder in human mouse rat

Cell Junction PathwayFinder in human mouse rat

Epithelial to Mesenchymal Transition (EMT) in human mouse rat

Interactions:

GeneGlobe Interaction Network for OCLN

STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

Selected Interacting proteins for OCLN (Q16625^{1, 2, 3} ENSP00000347379⁴) via UniProtKB, MINT, STRING, and/or I2D (see all 60)

Interactant		Interaction Details
GeneCard	External ID(s)	Interaction Details
CSNK1E	P49674¹^,²^,³, ENSP00000352929⁴	EBI-2903088,EBI-749343 MINT-1892115 MINT-1892205 MINT-1892281 MINT-1892254 MINT-1892301 MINT-1892321 I2D: score=2 STRING: ENSP00000352929
SNW1	Q13573²^,³, ENSP00000261531⁴	MINT-1892144 I2D: score=1 STRING: ENSP00000261531
ULK1	O75385²^,³, ENSP00000324560⁴	MINT-1892167 I2D: score=1 STRING: ENSP00000324560
CSNK2B	P67870³, ENSP00000365025⁴	I2D: score=1 STRING: ENSP00000365025
ENSG00000224774	P67870³, ENSP00000415615⁴	I2D: score=1 STRING: ENSP00000415615

About this table

Gene Ontology (GO): Selected biological process terms (see all 7): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0006461	protein complex assembly	TAS	10749869
GO:0006915	apoptotic process	TAS	--
GO:0006921	cellular component disassembly involved in execution phase of apoptosis	TAS	--
GO:0045216	cell-cell junction organization	IMP	--
GO:0046498	S-adenosylhomocysteine metabolic process	IEA	--

OCLN for ontologies About GeneDecksing

(Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
About This Section

TryGeneCards Plus

Browse Small Molecules at EMD Millipore

Browse drugs & compounds from Enzo Life Sciences

Browse compounds at ApexBio

Browse Tocris compounds for OCLN

(Secondary structures according to fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
Conferences by KenesGroup, exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, and/or QIAGEN )
About This Section

TryGeneCards Plus

REFSEQ mRNAs for OCLN gene (3 alternative transcripts):

NM_001205254.1 NM_001205255.1 NM_002538.3

Unigene Cluster for OCLN:

Occludin

Hs.592605 [show with all ESTs]

Unigene Representative Sequence: NM_002538

7 Ensembl transcripts including schematic representations, and UCSC links where relevant:

ENST00000355237(uc003jwu.3 uc003jwv.4 uc021xzq.1 uc021xzr.1 uc021xzs.1 uc021xzt.1)

ENST00000396442 ENST00000514370 ENST00000510666 ENST00000380766 ENST00000538151

ENST00000542132

miRNA Products:		Block miRNA regulation of human, mouse, rat OCLN using miScript Target Protectors
		Selected qRT-PCR Assays for microRNAs that regulate OCLN (see all 59):
		hsa-miR-124* hsa-miR-21* hsa-miR-607 hsa-miR-429 hsa-miR-500a hsa-miR-371-5p hsa-miR-570 hsa-miR-548l

		SwitchGear 3'UTR luciferase reporter plasmid: OCLN 3' UTR sequence

Inhib. RNA Products:		OriGene RNAi products in human, mouse, rat for OCLN
		Predesigned siRNA for gene silencing in human, mouse, rat OCLN

Clone Products:		OriGene clones in human, mouse for OCLN (see all 4) OriGene ORF clones in mouse, rat for OCLN OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
		GenScript: all cDNA clones in your preferred vector (see all 10): OCLN (XM_003118545)
		DNA2.0 Custom Codon Optimized Gene Synthesis Service for OCLN
		Search Vector BioLabs for ready-to-use adenovirus/AAV for human, mouse, rat OCLN

Primer Products:		OriGene qPCR primer pairs and template standards for OCLN
		Pre-validated RT² qPCR Primer Assay in human, mouse / rat OCLN
		QuantiTect SYBR Green Assays in human, mouse, rat OCLN
		QuantiFast Probe-based Assays in human, mouse, rat OCLN

Additional mRNA sequence:

AB451306.1 AB451437.1 AK290697.1 AK311037.1 AK313501.1 BC029886.1 DQ786238.1 FJ786083.1

FJ786084.1 GQ225096.1 GQ225097.1 GQ225098.1 U49184.1 U53823.1 Z70716.1

12 DOTS entries:

DT.456071 DT.100741549 DT.453238 DT.120845321 DT.438797 DT.100749715 DT.40118471 DT.120845253

DT.120845091 DT.120845165 DT.120845331 DT.100018590

GeneLoc Exon Structure

5 Alternative Splicing Database (ASD) splice patterns (SP) for OCLN About this scheme

ExUns:	1	^	2a	·	2b	^	3	^	4	^	5	^	6a	·	6b	·	6c	^	7	^	8	^	9	^	10
SP1:
SP2:									-
SP3:
SP4:			-		-		-		-
SP5:							-		-

ECgene alternative splicing isoforms for OCLN

(RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE ¹MOPED, ²PaxDb, and ³MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section

TryGeneCards Plus

OCLN expression in normal human tissues (normalized intensities) OCLN embryonic expression: see
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS <intensity>^2/3
CGAP TAG: --

About this image

OCLN expression in embryonic tissues and stem cells About this table

Data from LifeMap, the Embryonic Development and Stem Cells Database
selected tissues (see all 11) fully expand


Tooth (Integumentary System) fully expand to see all 6 entries
Early Ameloblasts Dental Enamel

Kidney (Urinary System) fully expand to see all 5 entries
Ureteric Bud Cells Ureteric Bud
Metanephros

Epithelial Cells
Early Ameloblasts Dental Enamel

Brain (Nervous System) fully expand to see all 3 entries
Mature Choroid Plexus Cells Choroid Plexus
Endothelial cells

Inner Cell Mass (Early Embryonic Tissues) fully expand to see all 2 entries
Human embryonic stem cells (family)

OCLN Protein expression data from MOPED¹, PaxDb² and MaxQB³:

About this image

OCLN Protein Expression

SOURCE GeneReport for Unigene cluster: Hs.592605

UniProtKB/Swiss-Prot: OCLN_HUMAN, Q16625

Tissue specificity: Localized at tight junctions of both epithelial and endothelial cells. Highly expressed in

kidney. Not detected in testis

Pathway & Disease-focused RT² Profiler PCR Arrays including OCLN:

Endothelial Cell Biology in human mouse rat

Tight Junctions in human mouse rat

Cancer PathwayFinder in human mouse rat

Cell Junction PathwayFinder in human mouse rat

Epithelial to Mesenchymal Transition (EMT) in human mouse rat

Primer Products:		OriGene qPCR primer pairs and template standards for OCLN
		Pre-validated RT² qPCR Primer Assay in human, mouse / rat OCLN
		QuantiTect SYBR Green Assays in human, mouse, rat OCLN
		QuantiFast Probe-based Assays in human, mouse, rat OCLN

In Situ
Assay Products:

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for OCLN

(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD , ⁵MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or ⁶Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
About This Section

TryGeneCards Plus

This gene was present in the common ancestor of chordates.

Orthologs for OCLN gene from Selected species (see all 11) About this table

Organism	Taxonomic classification	Gene	Description	Human Similarity	Orthology Type	Details
mouse (Mus musculus)	Mammalia	Ocln^{1 , 5}	occludin^{1, 5}	85.55(n)¹ 89.4(a)¹		13 (53.23 cM)⁵ 18260¹ NM_008756.2¹ NP_032782.1¹ 100496507⁵
chicken (Gallus gallus)	Aves	OCLN⁶	Gallus gallus occludin (OCLN), mRNA.	42(a)	1 ↔ 1	AADN03020404.1(184-3512)
lizard (Anolis carolinensis)	Reptilia	OCLN⁶	occludin	64(a)	1 ↔ 1	2(16949470-16984218)
tropical clawed frog (Xenopus tropicalis)	Amphibia	ocln¹	occludin	62.95(n) 58.57(a)		548702 NM_001015948.1 NP_001015948.1
zebrafish (Danio rerio)	Actinopterygii	oclna¹	occludin a	54.03(n) 48.12(a)		405757 NM_212832.2 NP_997997.2

ENSEMBL Gene Tree for OCLN (if available)
TreeFam Gene Tree for OCLN (if available)

(Paralogs according to ¹HomoloGene,
²Ensembl, and ³SIMAP, Pseudogenes according to ⁴Pseudogene.org Build 68)
About This Section

TryGeneCards Plus

Paralogs for OCLN gene

OCLN for paralogs About GeneDecksing

1 Pseudogenes.org Pseudogene for OCLN
PGOHUM00000260005

(SNPs/Variants according to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, ⁴UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
About This Section

TryGeneCards Plus

SNP ID	Valid	Clinical significance	Chr 5 pos	Sequence	#	AA Chg	Type	More	#	Allele freq	Pop	Total sample	More
Genomic Data					Transcription Related Data				Allele Frequencies
				--	--	--		--	--
VAR_064910⁴		Band-like calcification with simplified gyration and polymicrogyria (BLCPMG)⁴	--	see VAR_064910	2	F S	mis⁴		0	--	--	--	--
rs74972448^1,2	F	--	68786200(+)	`CACTCG/ATGTGT`	1	--	us2k¹		1		EA	120
rs78917696^1,2	C	--	68786213(+)	`ACAGAG/TTAGGC`	2	--	us2k¹		0	--	--	--	--
rs77460048^1,2	C	--	68786214(+)	`CAGATA/TAGGCA`	2	--	us2k¹		0	--	--	--	--
rs73116892^1,2	C,F	--	68786288(+)	`CACAAC/TATGTC`	1	--	us2k¹		2		WA	120
rs141198263^1,2		--	68786406(+)	`AAAACA/GTATGT`	1	--	us2k¹		0	--	--	--	--
rs187160479^1,2		--	68786439(+)	`ATGTTC/GATAGT`	1	--	us2k¹		0	--	--	--	--
rs191203447^1,2	C	--	68786511(+)	`TTAAAC/TGAAAT`	1	--	us2k¹		0	--	--	--	--
rs115190810^1,2	C	--	68786512(+)	`TAAACA/GAAATG`	1	--	us2k¹		0	--	--	--	--
rs114471020^1,2	C,F	--	68786734(+)	`GGAGAA/GGGGAA`	2	--	us2k¹		0	--	--	--	--

HapMap Linkage Disequilibrium report for OCLN (68788119 - 68853931 bp)

Structural Variations
Database of Genomic Variants (DGV) Selected variations for OCLN (see all 14): About this table

Variant ID	Type	Subtype	PubMed ID
esv2669461	CNV	Deletion	23128226
nsv471498	CNV	Duplication	19718026
nsv329061	CNV	Insertion	16902084
esv32846	CNV	Loss	17666407
nsv821643	CNV	Loss	15273396
nsv881771	CNV	Loss	21882294
nsv881768	CNV	Loss	21882294
esv1009388	CNV	Gain	20482838
dgv6086n71	CNV	Gain	21882294
nsv881770	CNV	Gain	21882294

Human Gene Mutation Database (HGMD): OCLN

Site Specific Mutation Identification with PCR Assays

SeqTarget long-range PCR primers for resequencing OCLN

DNA2.0 Custom Variant and Variant Library Synthesis for OCLN

(in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Conferences by KenesGroup, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
About This Section

TryGeneCards Plus

OMIM gene information: 602876
OMIM disorders: 251290
UniProtKB/Swiss-Prot: OCLN_HUMAN, Q16625

Band-like calcification with simplified gyration and polymicrogyria (BLCPMG) [MIM:251290]: A neurologic

disorder with characteristic clinical and neuroradiologic features that mimic intrauterine TORCH infection in the

absence of evidence of infection. Affected individuals have congenital microcephaly, intracranial calcifications,

and severe developmental delay. Note=The disease is caused by mutations affecting the gene represented in this

entry

Selected diseases for OCLN (see all 36): About MalaCards

pseudo-torch syndrome torch syndrome polymicrogyria cerebral artery occlusion

goldenhar syndrome brain glioma cholecystitis vascular dementia

brain edema arteriovenous malformation hepatitis c virus vaginitis

spinal muscular atrophy muscular atrophy diarrhea microcephaly

dementia diabetic retinopathy labyrinthitis hepatitis c

6 diseases from the University of Copenhagen DISEASES database for OCLN:

Brain edema Diabetic retinopathy pseudo-TORCH syndrome Inflammatory bowel disease

Cerebrovascular accident Brain glioma

OCLN for disorders About GeneDecksing

Congresses - knowledge worth sharing:
Alzheimer's & Parkinson's Diseases Congress (ADPD) 18 - 22 March 2015

Export disorders for OCLN gene to outside databases

(in PubMed. Associations of this gene to articles via ¹Entrez Gene, ²UniProtKB/Swiss-Prot, ³HGNC, ⁴GAD, ⁵PharmGKB, ⁶HMDB, ⁷DrugBank, ⁸UniProtKB/TrEMBL, ⁹ Novoseek, and/or ¹⁰fRNAdb)
About This Section

TryGeneCards Plus

PubMed articles for OCLN gene, integrated from 10 sources (see all 143):
(articles sorted by number of sources associating them with OCLN)

Utopia: connect your pdf to the dynamic
world of online information

Interspecies diversity of the occludin sequence: cDNA cloning of human, mouse, dog, and rat-kangaroo homologues. (PubMed id 8601611)^{1, 2, 3} Ando-Akatsuka Y.... Tsukita S. (J. Cell Biol. 1996)
Recessive mutations in the gene encoding the tight junction protein occludin cause band-like calcification with simplified gyration and polymicrogyria. (PubMed id 20727516)^{1, 2} O'Driscoll M.C.... Crow Y.J. (Am. J. Hum. Genet. 2010)
Splicing diversity of the human OCLN gene and its biological significance for hepatitis C virus entry. (PubMed id 20463075)^{1, 2} Kohaar I.... Prokunina-Olsson L. (J. Virol. 2010)
PKC eta regulates occludin phosphorylation and epithelial tight junction integrity. (PubMed id 19114660)^{1, 2} Suzuki T.... Rao R. (Proc. Natl. Acad. Sci. U.S.A. 2009)
Density-enhanced phosphatase 1 regulates phosphorylation of tight junction proteins and enhances barrier function of epithelial cells. (PubMed id 19332538)^{1, 2} Sallee J.L. and Burridge K. (J. Biol. Chem. 2009)
Phosphorylation of Tyr-398 and Tyr-402 in occludin prevents its interaction with ZO-1 and destabilizes its assembly at the tight junctions. (PubMed id 19017651)^{1, 2} Elias B.C.... Rao R. (J. Biol. Chem. 2009)
Identification and analysis of occludin phosphosites: a combined mass spectrometry and bioinformatics approach. (PubMed id 19125584)^{1, 2} Sundstrom J.M.... Antonetti D.A. (J. Proteome Res. 2009)
Large-scale phosphoproteome analysis of human liver tissue by enrichment and fractionation of phosphopeptides with strong anion exchange chromatography. (PubMed id 18318008)^{1, 2} Han G.... Gu J. (Proteomics 2008)
Human protein factory for converting the transcriptome into an in vitro-expressed proteome. (PubMed id 19054851)^{1, 2} Goshima N.... Nomura N. (Nat. Methods 2008)
ATM and ATR substrate analysis reveals extensive protein networks responsive to DNA damage. (PubMed id 17525332)^{1, 2} Matsuoka S.... Elledge S.J. (Science 2007)

(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

TryGeneCards Plus

		AND	OR
Aliases
Free Text

Query String		PubMed OMIM NCBI Bookshelf
	(Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
About This Section

TryGeneCards Plus

Entrez Gene: 100506658	HGNC: 8104	Ensembl:ENSG00000197822	euGenes: HUgn100506658	ECgene: OCLN	Kegg: 100506658
H-InvDB: OCLN

(According to HUGE)
About This Section

TryGeneCards Plus

(According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
About This Section

TryGeneCards Plus

Name	Description
PharmGKB entry for OCLN	Pharmacogenomics, SNPs, Pathways
Wikipedia	http://en.wikipedia.org/wiki/Occludin

Intellectual Property for OCLN gene
(Patent information from GeneIP, Licensable technologies from WIS Yeda, Salk, Tufts, IP news from LifeMap Sciences, Inc.)

About This Section

TryGeneCards Plus

Patent Information for OCLN gene:
Search GeneIP for patents involving OCLN

GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search

(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript, and ESI BIO, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
Advanced Cell Diagnostics, Animal models from inGenious Targeting Laboratory, genOway)
About This Section

TryGeneCards Plus

	Browse Kits and Assays available from EMD Millipore
	EMD Millipore Mono- and Polyclonal Antibodies for the study of OCLN
	Browse Small Molecules at EMD Millipore
	Browse Purified and Recombinant Proteins at EMD Millipore

	EMD Millipore genomic analysis products

	Antibodies for OCLN (Occludin)		Browse Cell Culture Products
	Browse ELISAs		Browse Flow Cytometry Kits
	Browse Primer Pairs		Browse Enzyme Activity Assays/Reagents
	Browse ELISpot/FluoroSpot Kits/Development Modules		Browse TFB/Immunoprecipitation Assays
	Browse Apoptosis Detection Kits/Reagents		Browse Ubiquitin Proteasome Pathway (UPP) Assay Kits/Reagents
	Browse DNA Damage/Repair Kits/Reagents		Browse Luminex Assays
	Browse Cell Selection/Detection Kits/Reagents		Browse Secondary Antibodies/Controls/Staining Reagents
	Browse Recombinant/Natural Proteins		Browse Stem Cell Products
	cDNA Clones for OCLN (Occludin)		Browse Proteome Profiler Antibody Arrays

	OriGene Antibodies for OCLN		OriGene RNAi products in human, mouse, rat for OCLN
	OriGene qPCR primer pairs and template standards for OCLN		OriGene Protein Over-expression Lysate for OCLN
	OriGene MassSpec something-or-other for OCLN		OriGene clones in human, mouse for OCLN
	Browse OriGene qSTAR qPCR primer pairs		OriGene Purified Protein for OCLN
	OriGene ORF clones in mouse, rat for OCLN		OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
	OriGene Custom Antibody Services for OCLN		OriGene Custom Protein Services for OCLN

	Block miRNA regulation of human, mouse, rat OCLN using miScript Target Protectors		SeqTarget long-range PCR primers for resequencing OCLN
	DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat OCLN		Predesigned siRNA for gene silencing in human, mouse, rat OCLN
	QuantiFast Probe-based Assays in human, mouse, rat OCLN		QuantiTect SYBR Green Assays in human, mouse, rat OCLN
	PCR Arrays including human, mouse / rat OCLN		Search Chromatin IP Primers for OCLN
	Pre-validated RT² qPCR Primer Assay in human, mouse / rat OCLN		GeneGlobe Interaction Network for OCLN
	Regulatory tfbs in OCLN promoter

	GenScript Custom Purified and Recombinant Proteins Services for OCLN		GenScript cDNA clones with any tag delivered in your preferred vector for OCLN
	GenScript Custom Assay Services for OCLN		GenScript Custom overexpressing Cell Line Services for OCLN
	CloneReady with Over 120,000 Genes		Gene Synthesis: Any Gene in Any Vector
	Vector-based siRNA and miRNA, Ready for Transfection		Gene Mutant Library, Variants up to 10^11
	Plasmid Preparation		Custom Peptide Services

Search for Antibodies & Assays

Search Tocris compounds for OCLN

	Browse Sino Biological Proteins
	Browse Sino Biological Cell Lysates
	cDNA Clones for OCLN
	4000+ Proteins
	Search Sino Biological for antibodies, proteins & pathways
	Protein Production Services
	Transfection Reagents
	Protein A/G/L resins
	Isotyping reagents

Search www.enzolifesciences.com for proteins, assays, substrates, inhibitors & antibodies

	Novus Tissue Slides
	OCLN antibodies
	OCLN proteins
	OCLN lysates

	Antibodies for OCLN
	See all of Abcam's Antibodies, Kits and Proteins for OCLN
	Custom Antibody / Protein Production Service
	Bulk Purchasing
	Advantages of Rabbit Monoclonal antibodies
	Abcam protocols and scientific support

Browse ProSpec Recombinant Proteins

	Proteins for OCLN
	Antibodies for OCLN
	ELISAs for OCLN
	CLIAs for OCLN

Browse ESI BIO Cell Lines and PureStem Progenitors for OCLN

	Gene Synthesis
	Protein Engineering
	Variant Library Synthesis
	Codon Optimization
	Protein Production and Purification

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for OCLN

	SwitchGear 3'UTR luciferase reporter plasmids for OCLN
	SwitchGear Promoter luciferase reporter plasmids for OCLN

ThermoFisher Antibodies for OCLN

Search Vector BioLabs for ready-to-use adenovirus/AAV for human, mouse, rat OCLN

	inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for OCLN
	inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for OCLN

LSBio Antibodies in human, mouse, rat for OCLN

Customized transgenic rodents for:

	Humanization
	Biomarker expression
	Off-target effect monitoring
	Translational medicine
	Tissue-specific gene expresssion
	Time-controlled gene expresssion

Browse compounds at ApexBio

GeneCards Homepage - Last full update: 7 May 2014 - Incrementals: 9 May 2014 , 2 Jun 2014 , 26 Jun 2014 , 30 Jun 2014

View Random Gene

Category			(GIFtS: )
GIFtS Group

The GeneCards human gene database gene index: 1 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

The GeneCards Human Gene Database

Developed at the Crown Human Genome Center, Department of Molecular Genetics, the Weizmann Institute of Science

Hot genes Disease genes OCLN gene at Home site.

Version: 3.12.136 22 July 2014
hostname: 356977-web1.xennexinc.com index build: 126 solr: 1.4

GeneCards Plus

Advanced Search

Export list of displayed genes

OCLN Gene

Occludin

Aliases
for OCLN gene

Summaries
for OCLN gene

Genomic Views
for OCLN gene

Proteins
for OCLN gene

OCLN Human Recombinant Protein Products:

OCLN Antibody Products:

OCLN Assay Products:

Domains /
Families
for OCLN gene

Function
for OCLN gene

Localization
for OCLN gene

Pathways & Interactions
for OCLN gene

Drugs & Compounds
for OCLN gene

Transcripts
for OCLN gene

Expression
for OCLN gene

Orthologs
for OCLN gene

Paralogs
for OCLN gene

Genomic Variants
for OCLN gene

Disorders / Diseases
for OCLN gene

Publications
for OCLN gene

External Searches for OCLN gene

Genome Databases showing OCLN gene

Other Databases showing OCLN gene

Specialized Databases showing OCLN gene

Intellectual Property
for OCLN gene

Products
for OCLN gene

View Random Gene

GeneCards Plus

Advanced Search

Export list of displayed genes

OCLN Gene

Occludin

Aliasesfor OCLN gene

Summariesfor OCLN gene

Genomic Viewsfor OCLN gene

Proteinsfor OCLN gene

OCLN Human Recombinant Protein Products:

OCLN Antibody Products:

OCLN Assay Products:

Domains /Familiesfor OCLN gene

Functionfor OCLN gene

Localizationfor OCLN gene

Pathways & Interactionsfor OCLN gene

Drugs & Compoundsfor OCLN gene

Transcriptsfor OCLN gene

Expression for OCLN gene

Orthologsfor OCLN gene

Paralogsfor OCLN gene

Genomic Variantsfor OCLN gene

Disorders / Diseasesfor OCLN gene

Publicationsfor OCLN gene

External Searches for OCLN gene

Genome Databases showing OCLN gene

Other Databases showing OCLN gene

Specialized Databases showing OCLN gene

Intellectual Propertyfor OCLN gene

Productsfor OCLN gene

View Random Gene

Aliases
for OCLN gene

Summaries
for OCLN gene

Genomic Views
for OCLN gene

Proteins
for OCLN gene

Domains /
Families
for OCLN gene

Function
for OCLN gene

Localization
for OCLN gene

Pathways & Interactions
for OCLN gene

Drugs & Compounds
for OCLN gene

Transcripts
for OCLN gene

Expression
for OCLN gene

Orthologs
for OCLN gene

Paralogs
for OCLN gene

Genomic Variants
for OCLN gene

Disorders / Diseases
for OCLN gene

Publications
for OCLN gene

Intellectual Property
for OCLN gene

Products
for OCLN gene