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Aliases & Descriptions for OBFC2B
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc
, and/or 7Ensembl,
8miRBase) About This Section
|
| Aliases |
|---|
| LP3587 2 | | MGC2731 1, 2 | | SSB1 2, 3, 5 |
| | | Descriptions |
|---|
| Single-stranded DNA-binding protein 1 3 | | oligonucleotide/oligosaccharide-binding fold containing 2B 2 |
|
| | Search outside databases for aliases for OBFC2B gene |
Summaries for OBFC2B(According to Entrez Gene,
Wikipedia's
Gene Wiki,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
| EntrezGene summary for OBFC2B: Single-stranded DNA (ssDNA)-binding proteins, such as OBFC2B, are ubiquitous and essential for a variety of DNA metabolic processes, including replication, recombination, and detection and repair of damage (Richard et al., 2008 [PubMed 18449195]).[supplied by OMIM] UniProtKB/Swiss-Prot: OFC2B_HUMAN, Q9BQ15Function: Plays an important role in DNA damage response. Influences diverse endpoints in the cellular DNA damage response, including cell-cycle checkpoint activation, recombinational repair and maintenance of genomic stability. Associates with DNA lesions, and enables the efficient activation of ATM and consequent phosphorylation of downstream proteins. Promotes RAD51-mediated strand exchange and may thus contribute to homologous recombination repair. Binds to single-stranded DNA, in particular to polypyrimidines |
Genomic Location for OBFC2B
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to
UCSC and
Ensembl,
Transcription factor binding sites according to
SABiosciences) About This Section
| Genomic View: UCSC Golden Path with GeneCards custom track
Transcription factor binding sites upstream to the OBFC2B gene 
Entrez Gene cytogenetic band: 12q13.2 Ensembl cytogenetic band: 12q13.2 HGNC cytogenetic band: 12q13.3OBFC2B Gene in genomic location: bands according to Ensembl, locations according to
(and/or Entrez Gene and/or Ensembl if different)
 GeneLoc gene densities for chromosome 12 GeneLoc Exon Structure GeneLoc location for GC12P054905:
(about GC identifiers)
Start:
|
54,904,410 bp from pter |
End:
|
54,909,905 bp from pter |
Size:
|
5,496 bases |
Orientation:
|
plus strand |
RefSeq DNA sequence:- NC_000012.10 NT_029419.11
| Proteins for OBFC2B
(According to
1UniProtKB,
and/or Ensembl,
Phosphorylation sites according to 2Phosphosite,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from Invitrogen,
Millipore,
Sigma-Aldrich,
R&D Systems,
Enzo Life Sciences,
Abnova,
OriGene and/or,
Abcam,
Biochemical Assays by
Invitrogen,
Millipore,
R&D Systems,
Cell Signaling Technology, and/or
Enzo Life Sciences,
Ontologies according to Gene
Ontology Consortium 01 Apr 2009 and
Entrez Gene,
Antibodies by Invitrogen,
Millipore,
Sigma-Aldrich,
R&D Systems,
Cell Signaling Technology,
Abcam,
Abnova, and/or
Novus Biologicals)
About This Section
| UniProtKB/Swiss-Prot: OFC2B_HUMAN, Q9BQ15 (See
protein sequence)Recommended Name: Oligonucleotide/oligosaccharide-binding fold-containing protein 2B Size: 211 amino acids; 22338 Da
Subunit: Directly interacts with ATM and RAD51
Subcellular location: Nucleus. Note=Localizes to nuclear foci following DNA damage. Foci formation is not cell-cyle dependent. Partial colocalization with RAD51 after ionizing radiation treatment
Sequence caution: Sequence=AAP34465.1; Type=Frameshift; Positions=169;
Secondary accessions: A6NDF8 Q6XYC8Alternative splicing: 2 isoforms: Q9BQ15-1 Q9BQ15-2 Post-translational modifications:
Phosphorylated by ATM in response to DNA damage. Phosphorylation prevents degradation by the proteasome, hence stabilization of the protein and accumulation within cells1
REFSEQ proteins: NP_076973.1
ENSEMBL proteins: ENSP00000267023 ENSP00000368862 ENSP00000369545
Human Recombinant Proteins               OriGene Purified Recombinant Human Protein: OBFC2B 
1 Gene Ontology (GO) cellular component term (links to tree view): | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0005634 | nucleus |
IEA | -- | About this table
Antibodies for OBFC2B: Assays for OBFC2B: | Protein
Domains/ Families for OBFC2B(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
| - Graphical View of Domain Structure for InterPro Entry Q9BQ15
ProtoNet protein and cluster: Q9BQ15 1 Blocks protein family: IPB004365 OB-fold nucleic acid binding domain
UniProtKB/Swiss-Prot: OFC2B_HUMAN, Q9BQ15Similarity: Belongs to the OBFC2 familySimilarity: Contains 1 OB DNA-binding domain | Gene Function for OBFC2B
(According to MGI Jun 06 2009, UniProtKB,
IUBMB,and/or Genatlas,
shRNA from
OriGene,
Sigma-Aldrich, RNAi from
Sigma-Aldrich,
RNAi Products,
Clones, and
Q-PCR Products
from Invitrogen,
Millipore,
OriGene, and/or
Abnova,
siRNAs from
Applied Biosystems,
SYBR primers from OriGene,
Cell-based Assays from Millipore,
Ontologies according to Gene Ontology Consortium 01 Apr 2009 via
Entrez Gene.)
About This Section
|               OriGene 29mer shRNA kit in GFP-retroviral vector: NM_024068
Applied Biosystems Silencer® siRNAs for OBFC2B
Sigma-Aldrich siRNA for OBFC2B  Sigma-Aldrich shRNA for OBFC2B  Explore Sigma-Aldrich super-pooled esiRNAs 
              OriGene GFP tagged cDNA clone in CMV expression vector: NM_024068                                  Myc/DDK tagged cDNA clone in CMV expression vector: NM_024068                                  untagged cDNA clone in CMV expression vector: NM_024068 
Primers: Browse
Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers               OriGene genome-wide validated SYBR primer pairs: NM_024068
UniProtKB/Swiss-Prot: OFC2B_HUMAN, Q9BQ15Function: Plays an important role in DNA damage response. Influences diverse endpoints in the cellular DNA damage response, including cell-cycle checkpoint activation, recombinational repair and maintenance of genomic stability. Associates with DNA lesions, and enables the efficient activation of ATM and consequent phosphorylation of downstream proteins. Promotes RAD51-mediated strand exchange and may thus contribute to homologous recombination repair. Binds to single-stranded DNA, in particular to polypyrimidines2 Gene Ontology (GO) molecular function terms (links to tree view): About this table | Pathways & Interactions for OBFC2B
(Pathways according to Invitrogen
(maps by GeneGo),
Millipore,
Cell Signaling Technology,
Sigma-Aldrich,
KEGG
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Proteins Network according to
SABiosciences,
Interactions according to 1UniProtKB,
2MINT, and/or
3STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Apr 2009 via
Entrez Gene.)
About This Section
|
5 Interacting proteins for OBFC2B (ENSP000002670233 Q9BQ151) via UniProtKB, MINT, and/or STRINGAbout this table
2 Gene Ontology (GO) biological process terms (links to tree view): | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0006281 | DNA repair |
IEA | -- | | GO:0006974 | response to DNA damage stimulus |
IEA | -- | About this table
|
Drugs & Compounds for OBFC2B(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
Sigma-Aldrich, Tocris Bioscience, and/or
Novoseek and Drugs according to
Enzo Life Sciences and/or
PharmGKB)
About This Section
|
Browse Tocris compounds for OBFC2B
|
Transcripts for OBFC2B(GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 219 Homo sapiens; Jun 2 2009) or GenBank, RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
non coding RNAs according to
RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from Invitrogen,
Millipore, and/or
Abnova,
siRNAs from Applied Biosystems,
Sigma-Aldrich,
shRNA from
Sigma-Aldrich,
OriGene,
Tagged/untagged cDNA clones from
OriGene, Expression Assays from Applied Biosystems) About This Section
|               OriGene 29mer shRNA kit in GFP-retroviral vector: NM_024068
Sigma-Aldrich siRNA for OBFC2B  Sigma-Aldrich shRNA for OBFC2B  Explore Sigma-Aldrich super-pooled esiRNAs 
Applied Biosystems Silencer® siRNAs: NM_024068 REFSEQ mRNAs for OBFC2B gene: NM_024068.3
Applied Biosystems TaqMan ® Gene Expression Assays: NM_024068               OriGene GFP tagged cDNA clone in CMV expression vector: NM_024068                                  Myc/DDK tagged cDNA clone in CMV expression vector: NM_024068                                  untagged cDNA clone in CMV expression vector: NM_024068  Additional cDNA sequence: AY203942.1 BC001079.2 BC006171.1 CR623688.1 CR625869.1 8 DOTS entries: DT.75116435 DT.121188260 DT.454705 DT.95109091 DT.92431301 DT.92431300 DT.121188266 DT.91840573 24/150 AceView cDNA sequences (see all 150
):AI708565 AV702469 BQ940268 BQ691894 CF128314 NM_024068 BC001079 BQ777329 AA364513 BM503783 BQ669133 BM828291 BE785134 AW731858 AW161556 CR625869 BM564784 CR623688 AA339573 BQ231282 CA417141 BG250628 BM451265 BU501063
highest scoring ESTs for OBFC2B:AA293809 AA394283 AA401958 AA411336 AI708565 AI760850 AW966310 AY203942 BC001079 BE548980 Unigene Cluster for OBFC2B: Oligonucleotide/oligosaccharide-binding fold containing 2B Hs.240170 [show with all ESTs]Unigene Representative Sequence: CR625869
GeneLoc Exon Structure
5/6 Alternative Splicing Database (ASD) splice patterns (SP) for OBFC2B (see all 6
)
| ExUns: | 1a | · | 1b | ^ | 2 | ^ | 3a | · | 3b | ^ | 4 | ^ | 5a | · | 5b | ^ | 6 | ^ | 7 | ^ | 8 | ^ | 9 | ^ | 10a | · | 10b | |
| SP1: | |   | |   | |   | |   | |   | - |   | - |   | |   | |   | |   | |   | |   | |   | |   | |
| SP2: | |   | |   | |   | |   | |   | |   | - |   | |   | |   | |   | |   | |   | |   | |   | |
| SP3: | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |
| SP4: | |   | |   | |   | - |   | - |   | - |   | - |   | |   | |   | |   | |   | |   | |   | |   | |
| SP5: | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   | |   |
About this scheme
ECgene alternative splicing isoforms for OBFC2B
3 Ensembl transcripts including schematic representations: ENST00000267023
ENST00000341463
ENST00000380198
|
Expression for OBFC2B
(Experimental results according to
1GeneNote
and GNF BioGPS,
probe sets-to-genes annotations according to
2GeneAnnot ,
3GeneTide ,
Sets of similar genes according to GeneDecks,
Electronic Northern calculations according to data from
UniGene (Build 219 Homo sapiens),
SAGE tags according to
CGAP,
plus additional links to
SOURCE, and/or
GNF
BioGPS, and/or
EXPOLDB, and/or
UniProtKB,
Expression Assays from
Applied Biosystems
)
About This Section
| OBFC2B expression in normal and diseased human tissues
Applied Biosystems TaqMan ® Gene Expression Assays for OBFC2B
1 / 2 / 3 3 probe-sets matching OBFC2B gene Data from
(Publications) and GNF BioGPS About these images About these images
CGAP SAGE TAG: GGAGTCTAAC
SOURCE GeneReport for Unigene cluster: Hs.240170
|
Orthologs for OBFC2B
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
and/or
5MGI Jun 06 2009,
with possible further links to
Flybase
and/or
WormBase,
Gene Trees according to Ensembl)
About This Section
|
Orthologs for OBFC2B gene from 5/10 species (see all 10
)
About this table Species with no ortholog for OBFC2B
ENSEMBL Gene Tree for OBFC2B | Paralogs for OBFC2B(Paralogs according to 1HomoloGene and 2Ensembl, Pseudogenes according to 3Pseudogene.org) About This Section
| Paralogs for OBFC2B gene
- OBFC2A2
Pseudogenes
- 1 related pseudogene
|
SNPs/Variants for OBFC2B(According to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE, and
UniProtKB,
Linkage Disequilibrium by HapMap,
Genotyping Reagents from
Applied Biosystems)
About This Section
|
HapMap Linkage Disequilibrium images for OBFC2B (up to first 250kb)
|
Disorders & Mutations for OBFC2B
(in which this Gene is Involved, According to
OMIM, UniProtKB,
PharmGKB,
Genatlas, GeneTests,
Blood group antigen gene mutations by BGMUT,
HGMD, GAD,
HuGE Navigator,
BCGD,
and/or TGDB.)
About This Section
|
OMIM: 612104
|
Medical News for OBFC2B(Possibly Related Articles in
Doctor's Guide)
About This Section
| -- |
Publications for OBFC2B (in
PubMed.
Associations of this gene to articles via
1Novoseek,
2HGNC,
3Entrez Gene,
4UniProtKB/Swiss-Prot,
5UniProtKB/TrEMBL,
6GAD, and/or
7PharmGKB)
About This Section
| 5 PubMed articles for OBFC2B gene: |
Search for OBFC2B
(in PubMed,
OMIM, and NCBI Bookshelf) About This Section
|
|
Genome Databases showing OBFC2B
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
and/or
H-InvDB)
About This Section
|
| Other Databases showing OBFC2B
(According to HUGE)
About This Section
| -- |
Specialized Databases showing OBFC2B(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL, Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot) About This Section
|
| About This Section
| --
| Services for OBFC2B(Reagents available from Applied Biosystems, Antibodies and assays by Cell
Signaling Technology, Abcam, Novus Biologicals, Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich, Enzo Life Sciences, and/or Tocris Bioscience) About This Section
| 
 Products for OBFC2B:

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| | | | Search Tocris compounds for OBFC2B |
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GeneCards Homepage - Last full update: 2 Jul 2009
Incremental update: 13 Oct 2009
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