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OBFC2A Gene

protein-coding   GIFtS: 44

GC02P192251
oligonucleotide/oligosaccharide-binding fold containing 2A
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
DKFZp667M1322 1, 2
FLJ13624 1, 2
FLJ22833 1, 2
MGC111163 1, 2
SSB2 2, 3, 5
Descriptions
Single-stranded DNA-binding protein 2 3
oligonucleotide/oligosaccharide-binding fold containing 2A 2
External Ids
HGNC: 262321
Entrez Gene: 648592
UniProtKB: Q96AH03
Ensembl: ENSG000001735597
Search outside databases for aliases for OBFC2A gene

Previous GC identifer: GC02P192369

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

EntrezGene summary for OBFC2A:
Single-stranded DNA (ssDNA)-binding proteins, such as OBFC2A, are ubiquitous and essential for a
variety of DNA metabolic processes, including replication, recombination, and detection and repair
of damage (Richard et al., 2008 [PubMed 18449195]).[supplied by OMIM]

UniProtKB/Swiss-Prot: OFC2A_HUMAN, Q96AH0
Function: Binds single-stranded nucleic acids (By similarity)

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the OBFC2A gene  

Entrez Gene cytogenetic band: 2q32.3   Ensembl cytogenetic band:  2q32.3   HGNC cytogenetic band: 2q32.3

OBFC2A Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 2         GeneLoc Exon Structure

GeneLoc location for GC02P192251:     (about GC identifiers)

Start:
192,251,107 bp from pter
End:
192,261,496 bp from pter
Size:
10,390 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000002.10  NT_005403.16  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
About This Section

UniProtKB/Swiss-Prot: OFC2A_HUMAN, Q96AH0 (See protein sequence)
Recommended Name: Oligonucleotide/oligosaccharide-binding fold-containing protein 2A  
Size: 204 amino acids; 22423 Da
Subcellular location: Nucleus (By similarity)
Secondary accessions: Q658Y8 Q9H5X6
Alternative splicing: 3 isoforms:  Q96AH0-1   Q96AH0-2   Q96AH0-3   

REFSEQ proteins: NP_001026886.1  

ENSEMBL proteins: 
ENSP00000310819 ENSP00000307968 ENSP00000387243 ENSP00000386605 


Human Recombinant Proteins 
Browse Drug Discovery Central at Invitrogen for human recombinant proteins
Browse Purified and Recombinant Proteins at Millipore
Browse Human Recombinant Proteins at Sigma-Aldrich  
Browse R&D Systems for human recombinant proteins
Browse recombinant and purified proteins available from Enzo Life Sciences
Browse Abcam for Recombinant Proteins
Human Recombinant Proteins from Abnova (FLJ22833)
                Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

1 Gene Ontology (GO) cellular component term (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005634 nucleus IEA--
About this table

Antibodies for OBFC2A: 
Browse Antibodies Central at Invitrogen
Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
Browse Antibodies at Sigma-Aldrich
Browse R&D Systems for Antibodies
Search for antibodies at Abcam and the World's Antibody Gateway (free search engine of over 150 antibody companies)
Browse Abnova for Monoclonal and Polyclonal Antibodies
Search Novus for antibodies for OBFC2A

Assays for OBFC2A: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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2 InterPro domains/families:
 IPR012340 NA-bd_OB-fold
 IPR004365 NA_bd_OB_tRNA-helicase


   GeneDecks  OBFC2A for the domains selected above  
About GeneDecksing

Graphical View of Domain Structure for InterPro Entry Q96AH0

ProtoNet protein and cluster: Q96AH0

2 Blocks protein families:
IPB000008 C2 domain
IPB004365 OB-fold nucleic acid binding domain


UniProtKB/Swiss-Prot: OFC2A_HUMAN, Q96AH0
Similarity: Belongs to the OBFC2 family
Similarity: Contains 1 OB DNA-binding domain

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (OBFC2A)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (FLJ22833)
               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 3): NM_022837

              Applied Biosystems Silencer® siRNAs for OBFC2A

              Sigma-Aldrich siRNA for OBFC2A  
                     Sigma-Aldrich shRNA for OBFC2A  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Invitrogen Clones for OBFC2A
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
              OriGene GFP tagged cDNA clone in CMV expression vector: NM_001031716
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_001031716
                                 untagged cDNA clones in CMV expression vector (see all 2): NM_001031716 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: NM_001031716

UniProtKB/Swiss-Prot: OFC2A_HUMAN, Q96AH0
Function: Binds single-stranded nucleic acids (By similarity)

1 Gene Ontology (GO) molecular function term (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0003676 nucleic acid binding IEA--
About this table

(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
About This Section


  --
(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences
Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for OBFC2A

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (OBFC2A)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (FLJ22833)
               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 3): NM_022837

              Sigma-Aldrich siRNA for OBFC2A  
                     Sigma-Aldrich shRNA for OBFC2A  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_001031716  

REFSEQ mRNAs for OBFC2A gene: 

NM_001031716.2   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_001031716  

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_001031716
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_001031716
                                 untagged cDNA clones in CMV expression vector (see all 2): NM_001031716 

Additional cDNA sequence: 

AK023686.1 AL832659.1 AL833684.1 BC017114.1 BC107723.1 CR593303.1 CR609297.1 CR620561.1 

17 DOTS entries:

DT.40215536  DT.95193944  DT.100025588  DT.40248617  DT.97842041  DT.95154842  DT.91941980  DT.95193962 
DT.433089  DT.120947742  DT.100003229  DT.100721227  DT.120947732  DT.120947752  DT.91848745  DT.92408251 
DT.99991466 

24/154 AceView cDNA sequences (see all 154 ):

BG435598 BM955845 CR593303 AA330290 BU618666 AI350615 AI699970 AL833684 
BG778066 AI199846 CD370809 CA429990 CD369785 BG535189 AI818692 AA320003 
AI860506 AK023686 BC017114 BX330608 BU928823 AA320108 AI473710 CA306529 

highest scoring ESTs for OBFC2A:

AI591110 AA320003 AA320108 AA744762 AA835797 AI199846 AI285347 AI473710 AI479247 AI520877 

Unigene Cluster for OBFC2A:

Oligonucleotide/oligosaccharide-binding fold containing 2A
Hs.591610  [show with all ESTs]
Unigene Representative Sequence: NM_001031716


GeneLoc Exon Structure

5/12 Alternative Splicing Database (ASD) splice patterns (SP) for OBFC2A (see all 12 )

ExUns: 1a · 1b · 1c · 1d ^ 2a · 2b ^ 3a · 3b · 3c · 3d ^ 4 ^ 5a · 5b · 5c ^ 6a · 6b ^ 7a · 7b ^ 8 ^ 9 ^ 10a · 10b · 10c · 10d · 10e · 10f
SP1:              -     -     -     -     -     -                                   -     -     -                       -     -                                 
SP2:                                      -     -                                   -     -     -                       -     -                                 
SP3:              -     -     -     -     -     -                                   -           -                                                               
SP4:              -     -     -     -     -     -                       -     -     -     -     -                                                               
SP5:              -     -     -     -     -     -                       -     -     -     -     -                       -     -                                 

About this scheme

ECgene alternative splicing isoforms for OBFC2A

4 Ensembl transcripts including schematic representations:
ENST00000307834  ENST00000307849  ENST00000410026  ENST00000409510  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

OBFC2A expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for OBFC2A

1 / 2 / 3

9 probe-sets matching OBFC2A gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank
77182_at2, 3 U95-E 1 1.00 1.00 0.96 0.97 AI591110 1.00 1.00 1.00 1
91414_at2, 3 U95-E 2 1.00 0.78 0.75 1.47 N72573 0.60 0.75 0.68 1

67329_at2, 3 U95-E 1 0.31 1.00 0.91 0.63 AA610377 0.60 1.00 0.82 1

219334_s_at2, 3 U133-A 1 1.00 1.00 -- -- NM_022837 0.40 1.00 0.76 1

233085_s_at2, 3 U133-B 1 1.00 1.00 -- -- AV734843 0.20 1.00 0.72 1

222872_x_at2, 3 U133-B 1 1.00 1.00 -- -- AU157541 0.40 1.00 0.76 1

219334_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

233085_s_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

222872_x_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
GeneDecks  OBFC2A for binary patterns associated with the probe-sets selected above  
About GeneDecksing
About this table    
Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: --

SOURCE GeneReport for Unigene cluster: Hs.591610
(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for OBFC2A gene from 5/7 species (see all 7 )
Organism Gene Locus Description Human
Similarity
NCBI accessions
chimpanzee
(Pan troglodytes)
OBFC2A1   -- oligonucleotide/oligosaccharide-binding fold containing more 99.84(n)
99.51(a)
744811  XM_001167708.1  XP_001167708.1 
cow
(Bos taurus)
OBFC2A1   -- oligonucleotide/oligosaccharide-binding fold containing more 93.63(n)
90.2(a)
613474  XM_871839.2  XP_876932.2 
rat
(Rattus norvegicus)
Obfc2a1   -- oligonucleotide/oligosaccharide-binding fold containing more 87.88(n)
83.33(a)
363227  NM_001014216.1  NP_001014238.1 
mouse
(Mus musculus)
Obfc2a1, 5 15
oligonucleotide/oligosaccharide-binding fold containing more1, 5 88.72(n)1
83.84(a)1
1090191  NM_028696.31  NP_082972.21 
 AI5064295  AI8525615  (see all 28)
chicken
(Gallus gallus)
OBFC2A1   -- oligonucleotide/oligosaccharide-binding fold containing more 74.46(n)
76.12(a)
424046  XM_421902.2  XP_421902.2 
About this table        Species with no ortholog for OBFC2A

ENSEMBL Gene Tree for OBFC2A
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

Paralogs for OBFC2A gene
OBFC2B2  

(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section


10/29 NCBI SNPs in OBFC2A are shown (see all 29 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 42)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr 2 posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
------------
--
rs126122561,2
H192258584(+) TTCACA/TCTGGC 1 T/S mis1 ese34Minor allele frequency- T:0.00EU EA WA 404
rs118981691,2
A,C,F,H192255911(+) TTATGT/GGTTAT 1 -- int15Minor allele frequency- G:0.07EU EA WA NA 506
rs101711061,2
A,C,F,H192252119(+) AAGTCG/CGGGGA 1 -- int14Minor allele frequency- C:0.04EU EA WA 418
rs101711451,2
A,C,F,H192252325(+) TACCCC/TGGTTT 1 -- int14Minor allele frequency- T:0.02EU EA WA 408
rs118924511,2
A,C,F,H192256011(+) AGTTAC/TGTGAA 1 -- int15Minor allele frequency- T:0.07EU EA WA NA 508
rs134071131,2
A,F,H192257028(+) GAAACG/ATTTCT 1 -- int14Minor allele frequency- A:0.05EU EA WA 418
rs134002981,2
A,F,H192255433(+) AGCAAC/TGAAAA 1 -- int14Minor allele frequency- T:0.02EU EA WA 418
rs101712091,2
C,H192252214(+) TTGAGG/AAGTTT 1 -- int13Minor allele frequency- A:0.00EU EA 282
rs123281271,2
F,H192252770(+) CCCTTC/TTCCAT 1 -- int1 trp34Minor allele frequency- T:0.02EU EA WA 414
rs118988311,2
A,C192253503(+) GTATAA/TTTGGA 1 -- int10--------
About this table

HapMap Linkage Disequilibrium images for OBFC2A (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

OMIM: 612103

(Possibly Related Articles in Doctor's Guide)
About This Section

  --

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
About This Section

6 PubMed articles for OBFC2A gene:
(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)
(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 64859 HGNC: 26232 AceView: FLJ22833 Ensembl:ENSG00000173559 euGenes: HUgn64859
ECgene: OBFC2A H-InvDB: OBFC2A
(According to HUGE)
About This Section

  --
(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

  --
(Available from WIS Yeda, Salk, Tufts)
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  --
(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for OBFC2A:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



  Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
  Invitrogen Antibodies  Invitrogen Assays
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 Predesigned and custom siRNAs for OBFC2A Browse antibodies at Sigma-Aldrich
 Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
 Lentivirus-delivered shRNAs for OBFC2A Browse small molecules at Sigma-Aldrich
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