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OBFC1 Gene

protein-coding   GIFtS: 51

GC10M105632
oligonucleotide/oligosaccharide-binding fold containing 1
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
AAF-44 2
FLJ22559 1, 2
OTTHUMP00000020417 2
OTTHUMP00000020418 2
RP11-541N10.2 2
RPA-32 2
bA541N10.2 1, 2
Descriptions
alpha accessory factor 44 2
oligonucleotide/oligosaccharide-binding fold containing 1 2
replication protein A 32 kDa subunit 2
External Ids
HGNC: 262001
Entrez Gene: 799912
UniProtKB: Q9H6683
Ensembl: ENSG000001079607
Search outside databases for aliases for OBFC1 gene

Previous GC identifer: GC10M105307

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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UniProtKB/Swiss-Prot: OBFC1_HUMAN, Q9H668
Function: May bind nucleic acids or oligosaccharides

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
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Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the OBFC1 gene  

Entrez Gene cytogenetic band: 10q24.33   Ensembl cytogenetic band:  10q24.33   HGNC cytogenetic band: 10q25.1

OBFC1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 10         GeneLoc Exon Structure

GeneLoc location for GC10M105632:     (about GC identifiers)

Start:
105,632,290 bp from pter
End:
105,667,956 bp from pter
Size:
35,667 bases
Orientation:
minus strand
RefSeq DNA sequence:
NC_000010.9  NT_030059.12  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: OBFC1_HUMAN, Q9H668 (See protein sequence)
Recommended Name: Oligonucleotide/oligosaccharide-binding fold-containing protein 1  
Size: 368 amino acids; 42119 Da
Secondary accessions: Q5TCZ0

REFSEQ proteins: NP_079204.2  

ENSEMBL proteins: 
ENSP00000224950 ENSP00000358779 


Human Recombinant Proteins 
Browse Drug Discovery Central at Invitrogen for human recombinant proteins
Browse Purified and Recombinant Proteins at Millipore
Browse Human Recombinant Proteins at Sigma-Aldrich  
Browse R&D Systems for human recombinant proteins
Browse recombinant and purified proteins available from Enzo Life Sciences
Recombinant Proteins from Abcam (OBFC1)
Human Recombinant Proteins from Abnova (OBFC1)
              OriGene Purified Recombinant Human Protein: OBFC1 

1 Gene Ontology (GO) cellular component term (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0005575 cellular_component ND--
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Antibodies for OBFC1: 
Browse Antibodies Central at Invitrogen
Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
Sigma-Aldrich Antibodies for OBFC1
Browse R&D Systems for Antibodies
Antibodies from Abcam (OBFC1), each with their AbpromiseSM
Monoclonal and Polyclonal Antibodies from Abnova (OBFC1)
Novus Biologicals Antibodies for OBFC1

Assays for OBFC1: 
Browse Invitrogen for biochemical assays
Browse Kits and Assays available from Millipore
Browse R&D Systems for biochemical assays
Browse biochemical assays available from Enzo Life Sciences

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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4 InterPro domains/families:
 IPR012340 NA-bd_OB-fold
 IPR004365 NA_bd_OB_tRNA-helicase
 IPR014647 UCP036950
 IPR015253 DUF1879


   GeneDecks  OBFC1 for the domains selected above  
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Graphical View of Domain Structure for InterPro Entry Q9H668

ProtoNet protein and cluster: Q9H668

1 Blocks protein family: IPB004365 OB-fold nucleic acid binding domain

(According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (OBFC1)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (OBFC1)
               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_024928

              Applied Biosystems Silencer® siRNAs for OBFC1

              Sigma-Aldrich siRNA for OBFC1  
                     Sigma-Aldrich shRNA for OBFC1  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Clones:Browse Clone Ranger at Invitrogen for clones
Browse Clones for the Expression of Recombinant Proteins Available from Millipore
              OriGene GFP tagged cDNA clone in CMV expression vector: NM_024928
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_024928
                                 untagged cDNA clone in CMV expression vector: NM_024928 

Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
              OriGene genome-wide validated SYBR primer pairs: BC017400

UniProtKB/Swiss-Prot: OBFC1_HUMAN, Q9H668
Function: May bind nucleic acids or oligosaccharides

2 Gene Ontology (GO) molecular function terms (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0003674 molecular_function ND--
GO:0003676 nucleic acid binding IEA--
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(Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
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3 Interacting proteins for OBFC1 (ENSP000002249503 Q9H6682) via UniProtKB, MINT, and/or STRING
InteractantInteraction Details
GeneCardExternal ID(s)
TUBGCP4Q9UGJ12MINT-66945
FAM5CENSP000003564323STRING (score=.706)
GAR1ENSP000002267963STRING (score=.7)
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1 Gene Ontology (GO) biological process term (links to tree view):

GO IDQualified GO termEvidencePubMed IDs
GO:0008150 biological_process ND--
About this table
(Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
About This Section

Browse drugs & compounds from Enzo Life Sciences
Browse Small Molecules at Sigma-Aldrich

Browse Tocris compounds for OBFC1

(GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView,
non coding RNAs according to RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from Invitrogen, Millipore, and/or Abnova,
siRNAs from Applied Biosystems, Sigma-Aldrich,
shRNA from Sigma-Aldrich, OriGene,
Tagged/untagged cDNA clones from OriGene,
Expression Assays from Applied Biosystems)
About This Section

Inhib.
RNA:
Invitrogen RNAi Products for gene knock-down (OBFC1)
Browse for Gene Knock-down Tools from Millipore
Abnova Chimera RNAi Products for Gene knock-down (OBFC1)
               OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 2): NM_024928

              Sigma-Aldrich siRNA for OBFC1  
                     Sigma-Aldrich shRNA for OBFC1  
                     Explore Sigma-Aldrich super-pooled esiRNAs  

Applied Biosystems Silencer® siRNAs: 

NM_024928  

REFSEQ mRNAs for OBFC1 gene: 

NM_024928.4   

Applied Biosystems TaqMan ® Gene Expression Assays: 

NM_024928  

              OriGene GFP tagged cDNA clone in CMV expression vector: NM_024928
                                 Myc/DDK tagged cDNA clone in CMV expression vector: NM_024928
                                 untagged cDNA clone in CMV expression vector: NM_024928 

Additional cDNA sequence: 

AK026212.1 AK057720.1 BC017400.2 CR600104.1 CR607240.1 CR609551.1 CR613200.1 CR617185.1 

9 DOTS entries:

DT.120029  DT.109159  DT.97844036  DT.91761464  DT.100025308  DT.100779229  DT.121283684  DT.95164581 
DT.95164583 

24/124 AceView cDNA sequences (see all 124 ):

CA311279 AA295283 AI288528 CR609551 BF969177 CA842906 BQ001796 AI332546 
CR617185 BQ012922 BM968640 BM675406 BM454693 CR600104 AI371170 W73832 
AI078563 CR607240 BM916320 AA505561 CA425283 BX952699 BM850716 AA143157 

highest scoring ESTs for OBFC1:

AA142978 AW964268 BC017400 BE019787 BF184393 BF797125 BG330913 BG765570 BI819194 BI908214 

Unigene Cluster for OBFC1:

Oligonucleotide/oligosaccharide-binding fold containing 1
Hs.62314  [show with all ESTs]
Unigene Representative Sequence: NM_024928


GeneLoc Exon Structure

5 Alternative Splicing Database (ASD) splice patterns (SP) for OBFC1

ExUns: 1a · 1b ^ 2a · 2b ^ 3a · 3b ^ 4 ^ 5a · 5b · 5c ^ 6 ^ 7 ^ 8 ^ 9 ^ 10 ^ 11a · 11b
SP1:              -                 -                       -                 -                           
SP2:                                                                          -                           
SP3:                                                                                                      
SP4:                                -                                                                     
SP5:              -                                                                                       

About this scheme

ECgene alternative splicing isoforms for OBFC1

2 Ensembl transcripts including schematic representations:
ENST00000224950  ENST00000369764  
(Experimental results according to 1GeneNote and GNF BioGPS,
probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
Expression Assays from Applied Biosystems )
About This Section

OBFC1 expression in normal and diseased human tissues

 Applied Biosystems TaqMan ® Gene Expression Assays for OBFC1

1 / 2 / 3

9 probe-sets matching OBFC1 gene


Affymetrix
probe-set
Array  GeneAnnot data GeneNote data GeneTide data
# genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

54050_at2, 3 U95-C 1 1.00 1.00 0.48 0.92 AI288528 0.60 1.00 0.82 1
60055_at2, 3 U95-C 1 1.00 1.00 0.78 1.11 W73844 0.60 1.00 0.82 1

58356_at2, 3 U95-B 1 1.00 1.00 0.77 1.25 AA142978 0.80 1.00 0.91 1
58359_g_at2, 3 U95-B 1 1.00 1.00 0.81 1.16 AA142978 0.80 1.00 0.91 1

57666_at2 U95-C 1 0.56 1.00 0.82 0.57 -- -- -- -- --

219100_at2, 3 U133-A 1 1.00 1.00 -- -- NM_024928 0.60 1.00 0.82 1

228781_at2 U133-B 1 1.00 1.00 -- -- -- -- -- -- --

219100_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

228781_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
GeneDecks  OBFC1 for binary patterns associated with the probe-sets selected above  
About GeneDecksing
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Data from (Publications) and GNF BioGPS
    About these images
About these images

CGAP SAGE TAG: ATTTCTGCTG

SOURCE GeneReport for Unigene cluster: Hs.62314
(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
About This Section


Orthologs for OBFC1 gene from 5/8 species (see all 8 )
Organism Gene Locus Description Human
Similarity
NCBI accessions
dog
(Canis familiaris)
OBFC11   -- oligonucleotide/oligosaccharide-binding fold containing more 88.3(n)
83.01(a)
477812  XM_535005.2  XP_535005.2 
chimpanzee
(Pan troglodytes)
OBFC11   -- oligonucleotide/oligosaccharide-binding fold containing more 99.64(n)
99.73(a)
450715  XM_508016.2  XP_508016.2 
cow
(Bos taurus)
OBFC11   -- oligonucleotide/oligosaccharide-binding fold containing more 86.29(n)
83.65(a)
514213  NM_001076849.1  NP_001070317.1 
rat
(Rattus norvegicus)
Obfc11   -- oligonucleotide/oligosaccharide-binding fold containing more 78.2(n)
70.84(a)
294025  NM_001011943.1  NP_001011943.1 
mouse
(Mus musculus)
Obfc11, 5 195
oligonucleotide/oligosaccharide-binding fold containing more1, 5 77.63(n)1
70.92(a)1
1086891  NM_175360.21  NP_780569.11 
 AA5901615  AI4134585  (see all 15)
About this table        Species with no ortholog for OBFC1

ENSEMBL Gene Tree for OBFC1
(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section

  --
(According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
About This Section


10/283 NCBI SNPs in OBFC1 are shown (see all 283 )
(Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 93)
ABGenomic DataTranscription DataAllele Frequencies
SNP IDValidChr 10 posSequenceRecsAA
Chg
TypeMoreRecsAllele
freq
PopTotal
sample
More
------------
rs9115471,2
A,C,F,H,O105629411(-) GCCTTT/CGGTCT 1 -- ut317Minor allele frequency- C:0.21EU EA WA NA 558
rs111918411,2
A,C,F,H105629601(+) tatgaT/Cagaaa 1 -- ut314Minor allele frequency- C:0.38EU EA WA 412
rs111918421,2
A,C,F,H105629798(+) cacttC/Tacccg 1 -- ut314Minor allele frequency- T:0.18EU EA WA 402
rs107867751,2
A,C,F,H105647306(+) CGGAGC/GAGGCA 1 S/C mis112Minor allele frequency- G:0.13EU EA WA 1256
rs24879991,2
A,C,F,H105649816(+) GTAAGC/TGGTGG 1 T/A mis114Minor allele frequency- T:0.16NA EU EA WA 1228
rs71009201,2
A,C,F,H105630968(+) tcacaC/Tttgtg 1 -- ut3115Minor allele frequency- T:0.40EU EA WA NA 1398
rs107488581,2
A,C,F,H105629504(+) AGGACT/GGAGGA 1 -- ut314Minor allele frequency- G:0.30EU EA WA 416
rs49174051,2
C,F,H105628222(+) GGAAAC/TAGCTT 1 -- ut3112Minor allele frequency- T:0.09EU EA WA 1250
rs38506731,2
C,F,H105669041(+) ACCTCC/TCCGGC 1 -- ng317Minor allele frequency- T:0.15EU EA WA NA 560
rs43872871,2
A,F,H105667887(+) CCCTGC/AGACCT 1 -- ut51 ese34Minor allele frequency- A:0.32EU EA WA 418
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HapMap Linkage Disequilibrium images for OBFC1 (up to first 250kb)

(in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
About This Section

Human Genome Epidemiology Navigator: OBFC1 (1 document)

(Possibly Related Articles in Doctor's Guide)
About This Section

  --

(in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
About This Section

7 PubMed articles for OBFC1 gene:
(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)
(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
About This Section

Entrez Gene: 79991 HGNC: 26200 AceView: OBFC1 Ensembl:ENSG00000107960 euGenes: HUgn79991
ECgene: OBFC1 H-InvDB: OBFC1
(According to HUGE)
About This Section

  --
(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

  --
(Available from WIS Yeda, Salk, Tufts)
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  --
(Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section



Products for OBFC1:
 TaqMan ® Gene Expression Assays
 TaqMan ® Genotyping Assays
  Free SNP selection tool



  Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
  Invitrogen Antibodies  Invitrogen Assays
  Invitrogen Clones  Invitrogen Q-PCR Products
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  Invitrogen Proteins / Assays / Screening Services  Search Invitrogen catalog for OBFC1-related products

 Millipore Custom Antibody & Bulk Services
 Millipore Preclinical / Clinical Development Services
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 Millipore Target Screening & Profiling Services


 Predesigned and custom siRNAs for OBFC1 Antibodies for OBFC1
 Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
 Lentivirus-delivered shRNAs for OBFC1 Browse small molecules at Sigma-Aldrich
 "Your Favorite Gene" Pathwaysfeedback


  
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 shRNA in GFP-retroviral vector
 Search Tocris compounds for OBFC1




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 Antibodies for OBFC1

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