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OAS3 Gene

protein-coding   GIFtS: 56

GC12P111838
2'-5'-oligoadenylate synthetase 3, 100kDa
(Previous name: 2'-5'-oligoadenylate synthetase 3 (100 kD) )
Symbol approved by the HUGO Gene Nomenclature Committee (HGNC) database
Services    
(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc , and/or 7Ensembl, 8miRBase)
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Aliases
EC 2.7.7.- 3
MGC133260 2
p100 2
p100OAS 3
Descriptions
(2-5')oligo(A) synthetase 3 3
2'-5'-oligoadenylate synthetase 3 (100 kD) 1, 2
2'-5'-oligoadenylate synthetase 3, 100kDa 2
2'-5'oligoadenylate synthetase 3 2
2'-5'oligoadenylate synthetase p100 2
2-5A synthetase 3 3
p100 OAS 3
External Ids
HGNC: 80881
Entrez Gene: 49402
UniProtKB: Q9Y6K53
Ensembl: ENSG000001113317
Search outside databases for aliases for OAS3 gene

Previous GC identifers: GC12P112271 GC12P112895 GC12P113159 GC12P111788

(According to Entrez Gene, Wikipedia's Gene Wiki,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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EntrezGene summary for OAS3:
This gene encodes an enzyme included in the 2', 5' oligoadenylate synthase family. This
enzyme is induced by interferons and catalyzes the 2', 5' oligomers of adenosine in
order to bind and activate RNase L. This enzyme family plays a significant role in the inhibition
of cellular protein synthesis and viral infection resistance. [provided by RefSeq]

UniProtKB/Swiss-Prot: OAS3_HUMAN, Q9Y6K5
Function: May play a role in mediating resistance to virus infection, control of cell growth,
differentiation, and apoptosis. OAS3 synthesizes preferentially dimeric 2',5'-oligoadenylate
molecules. GTP can be an alternative substrate

Gene Wiki entry for OAS3

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to UCSC and Ensembl, Transcription factor binding sites according to SABiosciences)
About This Section

Genomic View:
UCSC Golden Path with GeneCards custom track

 Transcription factor binding sites upstream to the OAS3 gene  

Entrez Gene cytogenetic band: 12q24.2   Ensembl cytogenetic band:  12q24.13   HGNC cytogenetic band: 12q24.2

OAS3 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)

GeneLoc gene densities for chromosome 12         GeneLoc Exon Structure

GeneLoc location for GC12P111838:     (about GC identifiers)

Start:
111,860,540 bp from pter
End:
111,900,791 bp from pter
Size:
40,252 bases
Orientation:
plus strand
RefSeq DNA sequence:
NC_000012.10  NT_009775.16  
(According to 1UniProtKB, and/or Ensembl, Phosphorylation sites according to 2Phosphosite, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Enzo Life Sciences, Abnova, OriGene and/or, Abcam,
Biochemical Assays by Invitrogen, Millipore, R&D Systems, Cell Signaling Technology, and/or Enzo Life Sciences, Ontologies according to Gene Ontology Consortium 01 Apr 2009 and Entrez Gene, Antibodies by Invitrogen, Millipore, Sigma-Aldrich, R&D Systems, Cell Signaling Technology, Abcam, Abnova, and/or Novus Biologicals)
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UniProtKB/Swiss-Prot: OAS3_HUMAN, Q9Y6K5 (See protein sequence)
Recommended Name: 2'-5'-oligoadenylate synthetase 3  
Size: 1087 amino acids; 121165 Da
Subunit: Monomer
Secondary accessions: Q9H3P5

Post-translational modifications:

  • View phosphorylation sites using PhosphoSite2


  • REFSEQ proteins: NP_006178.2  

    ENSEMBL proteins: 
    ENSP00000228928 


    Human Recombinant Proteins 
    Browse Drug Discovery Central at Invitrogen for human recombinant proteins
    Browse Purified and Recombinant Proteins at Millipore
    Browse Human Recombinant Proteins at Sigma-Aldrich  
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Recombinant Proteins from Abcam (OAS3)
    Browse Abnova for Human Recombinant Proteins
                    Browse Origene for full length recombinant human proteins expressed in human HEK293 cells 

    1 Gene Ontology (GO) cellular component term (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005792 microsome TAS2440675
    About this table

    Antibodies for OAS3: 
    Browse Antibodies Central at Invitrogen
    Browse Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Browse Antibodies at Sigma-Aldrich
    Browse R&D Systems for Antibodies
    Antibodies from Abcam (OAS3), each with their AbpromiseSM
    Monoclonal and Polyclonal Antibodies from Abnova (OAS3)
    Search Novus for antibodies for OAS3

    Assays for OAS3: 
    Browse Invitrogen for biochemical assays
    Browse Kits and Assays available from Millipore
    Browse R&D Systems for biochemical assays
    Browse biochemical assays available from Enzo Life Sciences

    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    4 InterPro domains/families:
     IPR002934 Nucleotidyltransferase
     IPR006116 2-5-oligoadenylate_synth_UB
     IPR018952 2-5-oligoAdlate_synth_1_dom2/C
     IPR006117 2-5-oligoadenylate_synth_CS


       GeneDecks  OAS3 for the domains selected above  
    About GeneDecksing

    Graphical View of Domain Structure for InterPro Entry Q9Y6K5

    ProtoNet protein and cluster: Q9Y6K5

    2 Blocks protein families:
    IPB002934 DNA polymerase
    IPB006117 2-5-oligoadenylate synthetase


    UniProtKB/Swiss-Prot: OAS3_HUMAN, Q9Y6K5
    Similarity: Belongs to the 2-5A synthetase family

    (According to MGI Jun 06 2009, UniProtKB, IUBMB,and/or Genatlas,
    shRNA from OriGene, Sigma-Aldrich, RNAi from Sigma-Aldrich,
    RNAi Products, Clones, and Q-PCR Products from Invitrogen, Millipore, OriGene, and/or Abnova, siRNAs from Applied Biosystems, SYBR primers from OriGene, Cell-based Assays from Millipore, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (OAS3)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (OAS3)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_006187

                  Applied Biosystems Silencer® siRNAs for OAS3

                  Sigma-Aldrich siRNA for OAS3  
                         Sigma-Aldrich shRNA for OAS3  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Clones:Browse Clone Ranger at Invitrogen for clones
    Browse Clones for the Expression of Recombinant Proteins Available from Millipore
                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_006187
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_006187
                                     untagged cDNA clone in CMV expression vector: NM_006187 

    Primers: Browse Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
                  OriGene genome-wide validated SYBR primer pairs: NM_006187

    UniProtKB/Swiss-Prot: OAS3_HUMAN, Q9Y6K5
    Function: May play a role in mediating resistance to virus infection, control of cell growth,
    differentiation, and apoptosis. OAS3 synthesizes preferentially dimeric 2',5'-oligoadenylate
    molecules. GTP can be an alternative substrate
    Catalytic activity: Binds double-stranded RNA and polymerizes ATP into PPP(A2'P5'A)N oligomers,
    which activate the latent RNase L that, when activated, cleaves single-stranded RNAs
    Induction: By interferons
    Enzyme Number (IUBMB): EC 2.7.7.- 

    Genatlas biochemistry entry for OAS3:
    2',5'-oligoadenylate synthetase 3,100kDa,ubiquitously expressed ,interferon induced

    4 Gene Ontology (GO) molecular function terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003723 RNA binding IEA--
    GO:0005524 ATP binding IEA--
    GO:0016740 transferase activity IEA--
    GO:0016779 nucleotidyltransferase activity IEA--
    About this table

    (Pathways according to Invitrogen (maps by GeneGo), Millipore, Cell Signaling Technology, Sigma-Aldrich, KEGG and/or UniProtKB,
    Sets of similar genes according to GeneDecks, Proteins Network according to SABiosciences, Interactions according to 1UniProtKB, 2MINT, and/or 3STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2009 via Entrez Gene.)
    About This Section


    1 Sigma-Aldrich "Your Favorite Gene" Pathway for  OAS3  (Your Favorite Gene powered by Ingenuity) 
     Role of Pattern Recognition Receptors in Recognition of Bacteria and Viruses

       GeneDecks  OAS3 for the pathways selected above  
    About GeneDecksing


    1 Interacting protein for OAS3 (ENSP000002289283) via UniProtKB, MINT, and/or STRING
    InteractantInteraction Details
    GeneCardExternal ID(s)
    --ENSP000003695523STRING (score=.796)
    About this table

    2 Gene Ontology (GO) biological process terms (links to tree view):

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006139 nucleobase, nucleoside, nucleotide and nucleic acid metabolic process TAS2440675
    GO:0006955 immune response IEA--
    About this table
    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, Sigma-Aldrich, Tocris Bioscience, and/or Novoseek and Drugs according to Enzo Life Sciences and/or PharmGKB)
    About This Section

    Browse drugs & compounds from Enzo Life Sciences
    Browse Small Molecules at Sigma-Aldrich

    Browse Tocris compounds for OAS3

    (GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 219 Homo sapiens; Jun 2 2009) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView,
    non coding RNAs according to RNAdb,
    ESTs according to GeneTide,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from Invitrogen, Millipore, and/or Abnova,
    siRNAs from Applied Biosystems, Sigma-Aldrich,
    shRNA from Sigma-Aldrich, OriGene,
    Tagged/untagged cDNA clones from OriGene,
    Expression Assays from Applied Biosystems)
    About This Section

    Inhib.
    RNA:
    Invitrogen RNAi Products for gene knock-down (OAS3)
    Browse for Gene Knock-down Tools from Millipore
    Abnova Chimera RNAi Products for Gene knock-down (OAS3)
                  OriGene 29mer shRNA kit in GFP-retroviral vector: NM_006187

                  Sigma-Aldrich siRNA for OAS3  
                         Sigma-Aldrich shRNA for OAS3  
                         Explore Sigma-Aldrich super-pooled esiRNAs  

    Applied Biosystems Silencer® siRNAs: 

    NM_006187  

    REFSEQ mRNAs for OAS3 gene: 

    NM_006187.2   

    Applied Biosystems TaqMan ® Gene Expression Assays: 

    NM_006187  

                  OriGene GFP tagged cDNA clone in CMV expression vector: NM_006187
                                     Myc/DDK tagged cDNA clone in CMV expression vector: NM_006187
                                     untagged cDNA clone in CMV expression vector: NM_006187 

    Additional cDNA sequence: 

    AB044545.1 AF063613.1 AK000608.1 AK292949.1 BC012015.1 BC113746.1 CR602049.1 

    10 DOTS entries:

    DT.211616  DT.436928  DT.95335870  DT.100792978  DT.121211619  DT.100721041  DT.40275249  DT.87016758 
    DT.91679771  DT.91872112 

    24/277 AceView cDNA sequences (see all 277 ):

    AK000608 BM842879 BU169707 BM808561 AA836757 BM471312 BG390192 BM810143 
    BU539874 NM_006187 AI650519 BM760794 BQ279127 CB109077 BU944625 CB123126 
    BU145349 BU539624 CA455330 AA312111 CA455349 BM913534 BM977099 BU956885 

    highest scoring ESTs for OAS3:

    AA135525 AA015623 AA015624 AA021105 AA021603 AA055014 AA102408 AA125906 AA127075 AA127938 

    Unigene Cluster for OAS3:

    2'-5'-oligoadenylate synthetase 3, 100kDa
    Hs.528634  [show with all ESTs]
    Unigene Representative Sequence: AB044545


    GeneLoc Exon Structure

    1 Ensembl transcript including schematic representation:
    ENST00000228928  
    (Experimental results according to 1GeneNote and GNF BioGPS,
    probe sets-to-genes annotations according to 2GeneAnnot , 3GeneTide , Sets of similar genes according to GeneDecks, Electronic Northern calculations according to data from UniGene (Build 219 Homo sapiens), SAGE tags according to CGAP, plus additional links to SOURCE, and/or GNF BioGPS, and/or EXPOLDB, and/or UniProtKB,
    Expression Assays from Applied Biosystems )
    About This Section

    OAS3 expression in normal and diseased human tissues

     Applied Biosystems TaqMan ® Gene Expression Assays for OAS3

    1 / 2 / 3

    5 probe-sets matching OAS3 gene


    Affymetrix
    probe-set
    Array  GeneAnnot data GeneNote data GeneTide data
    # genes Sensitivity Specificity Correlation Length Gb_Accession Consensus Uniqueness Score Rank

    64450_at2, 3 U95-C 1 1.00 1.00 1.00 1.00 AA135525 1.00 1.00 1.00 1

    218400_at2, 3 U133-A 1 1.00 1.00 -- -- NM_006187 0.60 1.00 0.82 1

    232666_at2, 3 U133-B 1 1.00 1.00 -- -- R13458 0.20 0.50 0.38 1

    218400_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --

    232666_at2 U133Plus2 1 1.00 1.00 -- -- -- -- -- -- --
    About this table
    Data from (Publications) and GNF BioGPS
        About these images
    About these images

    CGAP SAGE TAG: GCGCTGGTAC

    SOURCE GeneReport for Unigene cluster: Hs.528634

    UniProtKB/Swiss-Prot: OAS3_HUMAN, Q9Y6K5
    Tissue specificity: Present at high level in placenta trophoblast

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD and/or 5MGI Jun 06 2009, with possible further links to Flybase and/or WormBase, Gene Trees according to Ensembl)
    About This Section


    Orthologs for OAS3 gene from 3 species
    Organism Gene Locus Description Human
    Similarity
    NCBI accessions
    dog
    (Canis familiaris)
    OAS31   -- 2'-5'-oligoadenylate synthetase 3, 100kDa 82.52(n)
    77.83(a)
    477490  XM_534688.2  XP_534688.2 
    chimpanzee
    (Pan troglodytes)
    OAS31   -- 2'-5'-oligoadenylate synthetase 3, 100kDa 99.57(n)
    99.36(a)
    452267  XM_509393.2  XP_509393.2 
    mouse
    (Mus musculus)
    Oas31, 5 5 (67.10 cM)5
    2'-5' oligoadenylate synthetase 31, 5 75.89(n)1
    70.66(a)1
    2467271  NM_145226.21  NP_660261.11 
     AB0675345  AF4538305  (see all 10)
    About this table        Species with no ortholog for OAS3

    ENSEMBL Gene Tree for OAS3
    (Paralogs according to 1HomoloGene
    and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
    About This Section

    Paralogs for OAS3 gene
    OAS22  OASL2  OAS12  

    (According to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, and UniProtKB, Linkage Disequilibrium by HapMap, Genotyping Reagents from Applied Biosystems)
    About This Section


    10/261 NCBI SNPs in OAS3 are shown (see all 261 )
    (Click for Applied Biosystems TaqMan ® Genotyping Assay)  (see all 108)
    ABGenomic DataTranscription DataAllele Frequencies
    SNP IDValidChr 12 posSequenceRecsAA
    Chg
    TypeMoreRecsAllele
    freq
    PopTotal
    sample
    More
    ------------
    --
    rs20721331,2
    A,C,F,H,O111893643(-) ATTCAA/GTCATA 1 -- ut3115Minor allele frequency- G:0.20EU EA WA NA 1394
    rs7399031,2
    A,C,F,H,O111893257(-) TAGTGT/CGGACA 1 -- ut31 ese38Minor allele frequency- C:0.20MN EU EA WA NA 738
    rs20105491,2
    A,C,F,H,O111893380(-) GAAACC/GAATAG 1 -- ut31 ese314Minor allele frequency- G:0.26MN EU EA WA NA 2708
    rs20106041,2
    A,C,F,H,O111892591(-) ATAAAG/CTATTT 1 -- ut318Minor allele frequency- C:0.21NA EA MN EU WA 854
    rs22859331,2
    A,C,F,H111871162(-) GGTTTG/CCTCCC 1 R/S mis19Minor allele frequency- C:0.14EA EU WA 2324
    --
    rs18593311,2
    A,C,F,H111860714(-) TGGCCA/C/G/
            
    CCCGG
    1 -- ut51 ese34EU EA WA 408
    rs123026551,2
    A,C,F,H111858889(+) ATCCAG/ATCTCA 1 -- ng518Minor allele frequency- A:0.15EU EA WA 834
    rs107447911,2
    A,C,F,H111894699(+) ACCAGA/GTAAGT 1 -- ut318Minor allele frequency- G:0.17EU EA WA 838
    rs107746791,2
    A,C,F,H111859131(+) AGTTTT/CCAACC 1 -- ng518Minor allele frequency- C:0.42EU EA WA 834
    rs11563611,2
    A,C,F,H111860366(-) AGAACG/ATATGG 1 -- ng516Minor allele frequency- A:0.25EU EA WA MN 1656
    About this table

    HapMap Linkage Disequilibrium images for OAS3 (up to first 250kb)

    (in which this Gene is Involved, According to OMIM, UniProtKB, PharmGKB, Genatlas, GeneTests, Blood group antigen gene mutations by BGMUT, HGMD, GAD, HuGE Navigator, BCGD, and/or TGDB.)
    About This Section

    OMIM: 603351

    Genetic Association Database: OAS3
    Human Genome Epidemiology Navigator: OAS3 (2 documents)

    (Possibly Related Articles in Doctor's Guide)
    About This Section

      --

    (in PubMed. Associations of this gene to articles via 1Novoseek, 2HGNC, 3Entrez Gene, 4UniProtKB/Swiss-Prot, 5UniProtKB/TrEMBL, 6GAD, and/or 7PharmGKB)
    About This Section

    10/25 PubMed articles for OAS3 gene (see all 25 ):
    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)
    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, and/or H-InvDB)
    About This Section

    Entrez Gene: 4940 HGNC: 8088 AceView: OAS3 Ensembl:ENSG00000111331 euGenes: HUgn4940
    ECgene: OAS3 H-InvDB: OAS3
    (According to HUGE)
    About This Section

      --
    (According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section

      --
    (Available from WIS Yeda, Salk, Tufts)
    About This Section

      --
    (Reagents available from Applied Biosystems, Antibodies and assays by Cell Signaling Technology, Abcam, Novus Biologicals,
    Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
    Enzo Life Sciences, and/or Tocris Bioscience)
    About This Section



    Products for OAS3:
     TaqMan ® Gene Expression Assays
     TaqMan ® Genotyping Assays
      Free SNP selection tool



      Invitrogen iPath Pathways  Invitrogen BLOCK-iT™ RNAi
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     Millipore Immunoassay Services
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     Predesigned and custom siRNAs for OAS3 Browse antibodies at Sigma-Aldrich
     Explore super-pooled esiRNAs Browse proteins at Sigma-Aldrich
     Lentivirus-delivered shRNAs for OAS3 Browse small molecules at Sigma-Aldrich
     "Your Favorite Gene" Pathwaysfeedback


      
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     Search Novus for antibodies for OAS3

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