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OAF Gene

protein-coding   GIFtS: 45
GCID: GC11P120081

OAF Homolog (Drosophila)

  See related diseases
at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
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Aliases
OAF Homolog (Drosophila)1 2
NS5ATP13TP22 3
HCV NS5A-Transactivated Protein 13 Target Protein 22 3
Out At First Protein Homolog2

External Ids:    HGNC: 287521   Entrez Gene: 2203232   Ensembl: ENSG000001842327   UniProtKB: Q86UD13   

Export aliases for OAF gene to outside databases

Previous GC identifers: GC11P119587 GC11P116021


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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GeneCards Summary for OAF Gene:
OAF (OAF homolog (Drosophila)) is a protein-coding gene. Diseases associated with OAF include hypercalcemia, and sinusitis.


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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RefSeq DNA sequence:
NC_000011.10  NT_033899.9  NC_018922.2  
Regulatory elements:
   Regulatory transcription factor binding sites in the OAF gene promoter:
         AML1a   HNF-3beta   Ik-3   RORalpha2   PPAR-alpha   AREB6   POU2F1   POU2F1a   FOXO1a   FOXO1   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidOAF promoter sequence
   Search Chromatin IP Primers for OAF

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat OAF


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 11q23.3   Ensembl cytogenetic band:  11q23.3   HGNC cytogenetic band: 11q23.3

OAF Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
OAF gene location

GeneLoc information about chromosome 11         GeneLoc Exon Structure

GeneLoc location for GC11P120081:  view genomic region     (about GC identifiers)

Start:
120,081,475 bp from pter      End:
120,101,041 bp from pter
Size:
19,567 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Cloud-Clone Corp.,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Cloud-Clone Corp., Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, and/or Cloud-Clone Corp.)
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UniProtKB/Swiss-Prot: OAF_HUMAN, Q86UD1 (See protein sequence)
Recommended Name: Out at first protein homolog precursor  
Size: 273 amino acids; 30688 Da

Explore the universe of human proteins at neXtProt for OAF: NX_Q86UD1

Explore proteomics data for OAF at MOPED


See OAF Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

REFSEQ proteins: NP_848602.1  
ENSEMBL proteins: 
 ENSP00000332613   ENSP00000431865  

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(According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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1 InterPro protein domain:
 IPR026315 Oaf

Graphical View of Domain Structure for InterPro Entry Q86UD1

ProtoNet protein and cluster: Q86UD1

UniProtKB/Swiss-Prot: OAF_HUMAN, Q86UD1
Similarity: Belongs to the OAF family


OAF for domains           About GeneDecksing


(According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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Phenotypes:
     3 GenomeRNAi human phenotypes for OAF:
 G2 arrest  Increased G2M DNA content  Synthetic lethal with Ras 

Animal Models:
   inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for OAF
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miRTarBase miRNAs that target OAF:
hsa-mir-124-3p (MIRT004880)

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Selected qRT-PCR Assays for microRNAs that regulate OAF (see all 16):
hsa-miR-548am hsa-miR-875-3p hsa-miR-324-3p hsa-miR-513a-5p hsa-miR-27a hsa-miR-128 hsa-miR-3681* hsa-miR-1297
SwitchGear 3'UTR luciferase reporter plasmidOAF 3' UTR sequence
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(According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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Subcellular locations from COMPARTMENTS: 

CompartmentConfidence
extracellular3
golgi apparatus1

(SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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    Custom Pathway & Disease-focused RT2 Profiler PCR Arrays for OAF
Interactions:

    Search GeneGlobe Interaction Network for OAF

(Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
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Browse Small Molecules at EMD Millipore
   Browse drugs & compounds from Enzo Life Sciences
  Browse compounds at ApexBio 

Browse Tocris compounds for OAF



(Secondary structures according to fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
Conferences by KenesGroup, exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, and/or QIAGEN )
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REFSEQ mRNAs for OAF gene: 
NM_178507.2  

Unigene Cluster for OAF:

OAF homolog (Drosophila)
Hs.445081  [show with all ESTs]
Unigene Representative Sequence: BC047726
3 Ensembl transcripts including schematic representations, and UCSC links where relevant:
ENST00000328965(uc001pxb.3) ENST00000531220 ENST00000534735
miRNA
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Selected qRT-PCR Assays for microRNAs that regulate OAF (see all 16):
hsa-miR-548am hsa-miR-875-3p hsa-miR-324-3p hsa-miR-513a-5p hsa-miR-27a hsa-miR-128 hsa-miR-3681* hsa-miR-1297
SwitchGear 3'UTR luciferase reporter plasmidOAF 3' UTR sequence
Inhib. RNA
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Predesigned siRNA for gene silencing in human, mouse, rat OAF
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Primer
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Pre-validated RT2 qPCR Primer Assay in human, mouse, rat OAF
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  QuantiFast Probe-based Assays in human, mouse, rat OAF

Additional mRNA sequence: 

AK021720.1 AY459296.1 BC047726.1 

11 DOTS entries:

DT.95113993  DT.112735  DT.120746885  DT.120746972  DT.95113989  DT.100783971  DT.100739501  DT.120746898 
DT.91758611  DT.96086312  DT.91904293 

Selected AceView cDNA sequences (see all 172):

NM_178507 CR603084 CA420752 CR606518 CD675070 CR609690 BU681607 CR617508 
AY459296 AI537268 CR610860 BX394807 BE677599 CR617263 AA317315 CR600676 
BC047726 BG033561 BI834411 BX281076 BQ640207 AI095045 CK724948 BG036222 

GeneLoc Exon Structure

3 Alternative Splicing Database (ASD) splice patterns (SP) for OAF    About this scheme

ExUns: 1a · 1b ^ 2 ^ 3 ^ 4a · 4b ^ 5 ^ 6
SP1:              -     -                           
SP2:              -     -     -                     
SP3:                                                


ECgene alternative splicing isoforms for OAF

(RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
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OAF expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS <intensity>2/3
CGAP TAG: AAGTACGAGG
OAF Expression
About this image


OAF expression in embryonic tissues and stem cells    About this table
Data from LifeMap, the Embryonic Development and Stem Cells Database
 selected tissues (see all 2) fully expand
 
 Blood (Hematopoietic System)
         Monocytes Peripheral Blood
 
 Liver (Hepatobiliary System)
OAF Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

OAF Protein Expression

SOURCE GeneReport for Unigene cluster: Hs.445081
    Custom PCR Arrays for OAF
Primer
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QuantiFast Probe-based Assays in human, mouse, rat OAF
In Situ
Assay Products:
 

 
Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for OAF

(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
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This gene was present in the common ancestor of animals.

Orthologs for OAF gene from Selected species (see all 13)    About this table
Organism Taxonomic
classification
Gene Description Human
Similarity
Orthology
Type
Details
mouse
(Mus musculus)
Mammalia Oaf1 , 5 OAF homolog (Drosophila)1, 5 86.04(n)1
88.62(a)1
  9 (24.24 cM)5
1026441  NM_178644.31  NP_848759.11 
 432212785 
chicken
(Gallus gallus)
Aves OAF1 OAF homolog (Drosophila) 78.64(n)
73.88(a)
  395161  NM_204500.1  NP_989831.1 
lizard
(Anolis carolinensis)
Reptilia OAF6
OAF homolog (Drosophila)
67(a)
1 ↔ 1
LGa(1194413-1224725)
tropical clawed frog
(Xenopus tropicalis)
Amphibia Str.25082 Transcribed sequence with weak similarity to protein more 75.72(n)    AL900016.2 
zebrafish
(Danio rerio)
Actinopterygii oafa1 OAF homolog a (Drosophila) 68.32(n)
61.57(a)
  571744  XM_695363.6  XP_700455.3 
fruit fly
(Drosophila melanogaster)
Insecta oaf1 out at first 57.26(n)
41.91(a)
  33435  NM_057692.2  NP_477040.1 


ENSEMBL Gene Tree for OAF (if available)
TreeFam Gene Tree for OAF (if available)

(Paralogs according to 1HomoloGene,
2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
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(SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
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Selected SNPs for OAF (see all 508)    About this table    
Genomic DataTranscription Related DataAllele Frequencies
SNP IDValidClinical
significance
Chr 11 posSequence#AA
Chg
TypeMore#Allele
freq
PopTotal
sample
More
----------
rs1513313981,2
C--119945668(+) ATCCAC/GCATCC 1 -- us2k10--------
rs4778631,2
C,F,O,H--120079884(-) ACATCA/CGGGAG 1 -- us2k129Minor allele frequency- C:0.14NS EA NA WA CSA 2774
rs1911209861,2
--120079906(+) CCTTAG/TGAGCC 1 -- us2k10--------
rs3728270351,2
C--120079954(+) TATGT-/TTTTG/T
TTTGTTTTG
TTTTG
1 -- us2k10--------
rs1385811121,2
--120079955(+) TATGT-/TTTTG 
        
TTTTG
1 -- us2k10--------
rs4768091,2
C,A,H--120080022(-) CACTGC/AACTCC 1 -- us2k19Minor allele frequency- A:0.18MN NA WA CSA 198
rs1837838331,2
C--120080029(+) AGTGGC/TGCAAT 1 -- us2k10--------
rs1469332981,2
C--120080063(+) TCCTGG/TGTTCA 1 -- us2k10--------
rs1883665631,2
--120080095(+) TCCCGA/GGTAGC 1 -- us2k10--------
rs1379481801,2
C--120080159(+) AGAGAC/TGGGAT 1 -- us2k10--------

HapMap Linkage Disequilibrium report for OAF (120081475 - 120101041 bp)

Structural Variations
     Database of Genomic Variants (DGV) 4 variations for OAF:    About this table    
Variant IDTypeSubtypePubMed ID
esv2745145CNV Deletion23290073
esv2745144CNV Deletion23290073
nsv518CNV Insertion18451855
nsv518464CNV Loss19592680

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(in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Conferences by KenesGroup, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
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3 diseases for OAF:    
About MalaCards
hypercalcemia    sinusitis    malaria

2 diseases from the University of Copenhagen DISEASES database for OAF:
Glottis carcinoma     Sinusitis

OAF for disorders           About GeneDecksing


Export disorders for OAF gene to outside databases

(in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
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PubMed articles for OAF gene integrated from 10 sources:
(articles sorted by number of sources associating them with OAF)
    Utopia: connect your pdf to the dynamic
world of online information

  1. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)1, 3 Strausberg R.L....Marra M.A. (Proc. Natl. Acad. Sci. U.S.A. 2002)
  2. hORFeome v3.1: a resource of human open reading frames representing over 10,000 human genes. (PubMed id 17207965)1 Lamesch P.... Vidal M. (Genomics 2007)
  3. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)2 Gerhard D.S.... Malek J. (Genome Res. 2004)
  4. (PubMed id )2 

(in PubMed, OMIM, and NCBI Bookshelf)
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 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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Entrez Gene: 220323 HGNC: 28752 AceView: NS5ATP13TP2 Ensembl:ENSG00000184232 euGenes: HUgn220323
ECgene: OAF H-InvDB: OAF

(According to HUGE)
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(According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
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NameDescription
PharmGKB entry for OAF Pharmacogenomics, SNPs, Pathways

(Patent information from GeneIP,
Licensable technologies from WIS Yeda, Salk, Tufts,
IP news from LifeMap Sciences, Inc.)
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Patent Information for OAF gene:
Search GeneIP for patents involving OAF

GeneCards and IP:
Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript, and ESI BIO, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
Advanced Cell Diagnostics, Animal models from inGenious Targeting Laboratory, genOway)
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 Search ThermoFisher Antibodies for OAF
 Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat OAF
 inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for OAF
 inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for OAF
 LSBio Antibodies in human, mouse, rat for OAF
Customized transgenic rodents for:
 Humanization
 Biomarker expression
 Off-target effect monitoring
 Translational medicine
 Tissue-specific gene expresssion
 Time-controlled gene expresssion
 Browse compounds at ApexBio
       
GeneCards Homepage - Last full update: 7 May 2014 - Incrementals: 9 May 2014 , 2 Jun 2014 , 26 Jun 2014 , 30 Jun 2014

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