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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

NXT1 Gene

protein-coding   GIFtS: 53
GCID: GC20P023331

NTF2-like export factor 1

(Previous name: NTX2-like export factor1 )
 Explore 2 diseases affiliated with
NXT1 via our new
 Human Malady Compendium 
Biological research products
for NXT1
    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section

Aliases
NTF2-Like Export Factor 11 2     Protein P152 3
MTR21 2 5     NTF2-Related Export Protein 12
P151 2     NUTF-Like Export Factor 12
NTX2-Like Export Factor11 2     

External Ids:    HGNC: 159131   Entrez Gene: 291072   Ensembl: ENSG000001326617   OMIM: 6058115   UniProtKB: Q9UKK63   

Export aliases for NXT1 gene to outside databases

Previous GC identifers: GC20P023319 GC20P023326 GC20P023279


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for NXT1:
The protein encoded by this gene is located in the nuclear envelope. It has protein similarity to nuclear transport
factor 2. This protein functions as a nuclear export factor in both RAN (Ras-related nuclear protein)- and CRM1
(required for chromosome region maintenance)-dependent pathways. It is found to stimulate the export of U1 snRNA in
RAN- and CRM1-dependent pathways and the export of tRNA and mRNA in a CRM1-independent pathway. The encoded protein
heterodimerizes with Tap protein and may regulate the ability of Tap protein to mediate nuclear mRNA export. The use
of alternate polyadenylation sites has been found for this gene. (provided by RefSeq, Jul 2008)

UniProtKB/Swiss-Prot: NXT1_HUMAN, Q9UKK6
Function: Stimulator of protein export for NES-containing proteins. Also plays a role in the nuclear export of U1
snRNA, tRNA, and mRNA. The NXF1-NXT1 heterodimer is involved in the export of HSP70 mRNA in conjunction with
ALYREF/THOC4 and THOC5

Gene Wiki entry for NXT1


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000020.10  NC_018931.1  NT_011387.8  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the NXT1 gene promoter:
         Elk-1   p53   Cdc5   POU3F2   Max   E2F-1   E2F   ZIC2/Zic2   Zic3   c-Myc   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidNXT1 promoter sequence
   Search SABiosciences Chromatin IP Primers for NXT1

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat NXT1


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 20p12-p11.2   Ensembl cytogenetic band:  20p11.21   HGNC cytogenetic band: 20p12-p11.2

NXT1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
NXT1 gene location

GeneLoc information about chromosome 20         GeneLoc Exon Structure

GeneLoc location for GC20P023331:  view genomic region     (about GC identifiers)

Start:
23,331,373 bp from pter      End:
23,335,414 bp from pter
Size:
4,042 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: NXT1_HUMAN, Q9UKK6 (See protein sequence)
Recommended Name: NTF2-related export protein 1  
Size: 140 amino acids; 15847 Da
Subunit: Heterodimer with NXF1. Interacts (via NTF2 domain) with NXF1. Stablizes the NTF2 domain of NXF1 by
heterodimerization. The formation of NXF1/NXT1 heterodimers is required for the NXF1-mediated nuclear mRNA export.
Preferentially binds Ran-GTP. Associates with NXF2, NXF3 and NXF5. Does not bind nucleoporins (NPC) directly, its
association to NPC is mediated by NXF1
Subcellular location: Nucleus. Cytoplasm. Note=Shuttles between the nucleus and the cytoplasm
2 PDB 3D structures from and Proteopedia for NXT1:
1JKG (3D)        1JN5 (3D)    

Explore the universe of human proteins at neXtProt for NXT1: NX_Q9UKK6

Post-translational modifications:

  • View modification sites using PhosphoSitePlus2
  • View neXtProt modification sites for NX_Q9UKK6

  • NXT1 Protein expression data from MOPED and PaxDb:    About this image 
    NXT1 Protein Expression
    REFSEQ proteins: NP_037380.1  
    ENSEMBL proteins: 
     ENSP00000254998  

    Human Recombinant Protein Products for NXT1: 
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    Uscn Proteins for NXT1

    Gene Ontology (GO): 2 cellular component terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005643nuclear pore IEA--
    GO:0005737cytoplasm IEA--

    NXT1 for ontologies           About GeneDecksing



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    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    NXT1 for domains           About GeneDecksing

    2 InterPro domains/families:
     IPR002075 NTF2
     IPR018222 Nuclear_transport_factor_2_euk

    Graphical View of Domain Structure for InterPro Entry Q9UKK6

    ProtoNet protein and cluster: Q9UKK6

    1 Blocks protein family: IPB002075 Nuclear transport factor 2 (NTF2)

    UniProtKB/Swiss-Prot: NXT1_HUMAN, Q9UKK6
    Similarity: Contains 1 NTF2 domain


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase, shRNA from OriGene, Sirion Biotech, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Sirion Biotech, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, Sirion Biotech, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section

    Molecular Function:

         UniProtKB/Swiss-Prot Summary: NXT1_HUMAN, Q9UKK6
    Function: Stimulator of protein export for NES-containing proteins. Also plays a role in the nuclear export of U1
    snRNA, tRNA, and mRNA. The NXF1-NXT1 heterodimer is involved in the export of HSP70 mRNA in conjunction with
    ALYREF/THOC4 and THOC5

         Gene Ontology (GO): 2 molecular function terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005515protein binding IPI19060904
    GO:0008536Ran GTPase binding IEA--
         
    NXT1 for ontologies           About GeneDecksing


    Phenotypes:
         2 GenomeRNAi human phenotypes for NXT1:
     Increased G1 DNA content  Increased number of mitotic ce 

    Animal Models:
       inGenious Targeting Laboratory - Customizable classic, inducible, and humanized mouse model solutions for NXT1 

    miRNA
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    Inhib. RNA
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    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section

    Unified GeneCards pathways  About this table 
    See pathways by source

    Super-pathwaycontained gene-specific pathways
    1mRNA surveillance pathway
    mRNA surveillance pathway1.00
    2Ribosome biogenesis in eukaryotes
    Ribosome biogenesis in eukaryotes1.00
    3RNA transport
    RNA transport1.00
    4Measles
    Influenza A0.35

    Pathway sources
    See GeneCards unified pathways
    Show all pathways



    4         Kegg Pathways  (Kegg details for NXT1):
        Ribosome biogenesis in eukaryotes
    RNA transport
    mRNA surveillance pathway
    Influenza A


    NXT1 for pathways           About GeneDecksing

    Interactions:

        SABiosciences Gene Network CentralTM Interacting Genes and Proteins Network for NXT1

    STRING Interaction Network Preview (showing 5 interactants - click image to see 13)

    5/21 Interacting proteins for NXT1 (Q9UKK61, 2, 3 ENSP000002549984) via UniProtKB, MINT, STRING, and/or I2D (see all 21)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    NXF1Q9UBU91, 2, 3, ENSP000002941724EBI-301889,EBI-398874 MINT-25019 I2D: score=8 STRING: ENSP00000294172
    NXF3Q9H4D51, 3, ENSP000003785044EBI-301889,EBI-750038 I2D: score=4 STRING: ENSP00000378504
    RANP628263, ENSP000003961274I2D: score=1 STRING: ENSP00000396127
    ALYREFQ86V812, ENSP000003318174MINT-7040261 STRING: ENSP00000331817
    PRKAB1Q9Y4783, ENSP000002293284I2D: score=1 STRING: ENSP00000229328
    About this table

    Gene Ontology (GO): 2 biological process terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006405RNA export from nucleus IEA--
    GO:0006611protein export from nucleus IEA--

    NXT1 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section

    NXT1 for compounds           About GeneDecksing

    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for NXT1

    1 HMDB Compound for NXT1    About this table
    CompoundSynonyms CAS #PubMed Ids
    Guanosine triphosphate5'-GTP (see all 10)86-01-1--
    1 Novoseek chemical compound relationship for NXT1 gene    About this table
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    leptomycin b 81.1 2 10567585 (1), 12743041 (1)

    Search CenterWatch for drugs/clinical trials and news about NXT1 

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, Sirion Biotech, microRNA from QIAGEN,
    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section

    REFSEQ mRNAs for NXT1 gene: 
    NM_013248.2  

    Unigene Cluster for NXT1:

    NTF2-like export factor 1
    Hs.516933  [show with all ESTs]
    Unigene Representative Sequence: BM809200
    1 Ensembl transcript including schematic representation, and UCSC links where relevant:
    ENST00000254998(uc002wsx.1)

    miRNA
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    Additional cDNA sequence: 

    AF156957.1 AK026360.1 BC000759.2 BC002687.2 BC003029.1 BC003410.1 

    3 DOTS entries:

    DT.314295  DT.95370113  DT.120773750 

    24/126 AceView cDNA sequences (see all 126):

    AI167905 BI913135 AA223284 BX113437 AA872518 BM552305 BM789812 BC002687 
    BU521391 AK026360 AW246842 BE893090 BQ187897 CB305847 BQ937662 CR620694 
    AW274482 AW082583 AW205842 BU173898 AI206974 BU602316 AI739032 Z41659 

    GeneLoc Exon Structure


    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    NXT1 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS
    CGAP TAG: TTGCTTCTTA
    NXT1 Expression
    About this image
    See NXT1 Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for NXT1

    SOURCE GeneReport for Unigene cluster: Hs.516933
        SABiosciences Custom PCR Arrays for NXT1

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    In Situ
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    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the common ancestor of animals.

    Orthologs for NXT1 gene from 7/20 species (see all 20)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Nxt11 , 5 NTF2-related export protein 11, 5 91.43(n)1
    98.57(a)1
      2 (73.53 cM)5
    564881  NM_001110159.11  NP_001103629.11 
     1486726015 
    chicken
    (Gallus gallus)
    Aves NXT2_CHICK6
    NTF2-related export protein 2
    81(a)
    1 → many
    4(13910739-13913891)
    lizard
    (Anolis carolinensis)
    Reptilia NXT26
    --
    72(a)
    1 → many
    GL343202.1(4342702-4344655)
    tropical clawed frog
    (Xenopus tropicalis)
    Amphibia Str.18562 Transcribed sequence with moderate similarity to protein more 75.84(n)    137720472 
    zebrafish
    (Danio rerio)
    Actinopterygii nxt26
    nuclear transport factor 2-like export factor 2
    57(a)
    1 → many
    14(29479556-29484083)
    fruit fly
    (Drosophila melanogaster)
    Insecta Nxt11 NTF2-related export protein 1 52.2(n)
    42.64(a)
      37769  NM_137989.2  NP_611833.1 
    worm
    (Caenorhabditis elegans)
    Secernentea nxt-16
    NTF2-related export protein
    22(a)
    1 → many
    I(2929779-2931363)


    ENSEMBL Gene Tree for NXT1 (if available)
    TreeFam Gene Tree for NXT1 (if available) 

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    Paralogs for NXT1 gene
    NXT22  
    1 SIMAP similar gene for NXT1 using alignment to 1 protein entry:     NXT1_HUMAN:
    NXT2

    NXT1 for paralogs           About GeneDecksing


    1 Pseudogenes.org Pseudogene for NXT1
    PGOHUM00000248372


    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/125 NCBI SNPs in NXT1 are shown (see all 125    About this table
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 20 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs746504131,2
    C--23329411(+) TGATCA/GTGACC 1 -- us2k10--------
    rs1411956921,2
    --23329437(+) CCTTAA/GAGTTG 1 -- us2k10--------
    rs749873891,2
    C--23329553(+) TTGGTG/TTCTGA 1 -- us2k10--------
    rs1924141111,2
    --23329587(+) TGCTAC/TAAATA 1 -- us2k10--------
    rs790776071,2
    F--23329628(+) GAGAGC/GAGGGA 1 -- us2k11Minor allele frequency- G:0.11EA 120
    rs1858341331,2
    --23329641(+) GAGTGC/TTGAGG 1 -- us2k10--------
    rs1507530411,2
    --23329682(+) CATGAC/TTTGAA 1 -- us2k10--------
    rs37612771,2
    C,F,A,H--23329694(+) CCAGGT/CTTGTC 1 -- us2k122Minor allele frequency- C:0.50EA NA NS WA CSA 1372
    rs1380334861,2
    --23329717(+) CAGTAC/TTTATT 1 -- us2k10--------
    rs37612781,2
    C,F,H--23329871(+) TGGAGC/ATGCAT 1 -- us2k112Minor allele frequency- A:0.08EA NS NA WA CSA 2254

    HapMap Linkage Disequilibrium report for NXT1 (23331373 - 23335414 bp)
    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
          Database of Genomic Variants (DGV) variations for NXT1: --

    SABiosciences Cancer Mutation PCR Assays
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    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    NXT1 for disorders           About GeneDecksing

    OMIM gene information: 605811    OMIM disorders: --

    2 diseases for NXT1:    About MalaCards
    conjunctivitis    influenza


    Export disorders for NXT1 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for NXT1 gene, integrated from 9 sources (see all 26):
    (articles sorted by number of sources associating them with NXT1)
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    1. NXT1 (p15) is a crucial cellular cofactor in TAP-dependent export of intron-containing RNA in mammalian cells. (PubMed id 11259602)1, 2, 3, 9 Guzik B.W.... Hammarskjold M.L. (2001)
    2. Identification of an NTF2-related factor that binds Ran-GTP and regulates nuclear protein export. (PubMed id 10567585)1, 2, 3, 9 Black B.E.... Paschal B.M. (1999)
    3. RanGTP-binding protein NXT1 facilitates nuclear export of different classes of RNA in vitro. (PubMed id 10848583)1, 2, 9 Ossareh-Nazari B....Dargemont C. (2000)
    4. Structural basis for the recognition of a nucleoporin FG repeat by the NTF2-like domain of the TAP/p15 mRNA nuclear export factor. (PubMed id 11583626)1, 2, 9 Fribourg S.... Conti E. (2001)
    5. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
    6. The DNA sequence and comparative analysis of human chromosome 20. (PubMed id 11780052)1, 2 Deloukas P....Rogers J. (2001)
    7. TAP (NXF1) belongs to a multigene family of putative RNA export factors with a conserved modular architecture. (PubMed id 11073998)1, 9 Herold A.... Izaurralde E. (2000)
    8. The Mex67p-mediated nuclear mRNA export pathway is conserved from yeast to human. (PubMed id 10228171)1, 9 Katahira J....Hurt E. (1999)
    9. A census of human soluble protein complexes. (PubMed id 22939629)1 Havugimana P.C....Emili A. (2012)
    10. Systematic and quantitative assessment of the ubiquiti n-modified proteome. (PubMed id 21906983)1 Kim W....Gygi S.P. (2011)

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    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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    Entrez Gene: 29107 HGNC: 15913 AceView: NXT1 Ensembl:ENSG00000132661 euGenes: HUgn29107
    ECgene: NXT1 Kegg: 29107 H-InvDB: NXT1

    (According to HUGE)
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    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
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    NameDescription
    PharmGKB entry for NXT1 Pharmacogenomics, SNPs, Pathways

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