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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

NTNG2 Gene

protein-coding   GIFtS: 54
GCID: GC09P135037

netrin G2

(Previous name: netrin G1 )
(Previous symbol: NTNG1)
 Explore 6 diseases affiliated with
NTNG2 via our new
 Human Malady Compendium 
Biological research products
for NTNG2
    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section

Aliases
Netrin G21 2     BA479K20.1 (Novel Protein)2
Lmnt21 2     Laminet 22
NTNG11 2     Laminet-23
Netrin G11 2     Netrin-G21
KIAA18571 3     LMNT23
LHLL93812     Laminet-23
BA479K20.11     

External Ids:    HGNC: 142881   Entrez Gene: 846282   Ensembl: ENSG000001963587   UniProtKB: Q96CW93   

Export aliases for NTNG2 gene to outside databases

Previous GC identifers: GC09P130318 GC09P132066 GC09P134026 GC09P104523


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

UniProtKB/Swiss-Prot: NTNG2_HUMAN, Q96CW9
Function: Promotes neurite outgrowth of both axons and dendrites (By similarity)

Gene Wiki entry for NTNG2


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000009.11  NC_018920.1  NT_035014.4  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the NTNG2 gene promoter:
         E2F-3a   E2F-4   E2F-5   E2F-2   C/EBPalpha   AREB6   CHOP-10   E2F   E2F-1   Pax-4a   
         Other transcription factors

Browse SwitchGear Promoter luciferase reporter plasmids
   Search SABiosciences Chromatin IP Primers for NTNG2

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat NTNG2


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 9q34   Ensembl cytogenetic band:  9q34.13   HGNC cytogenetic band: 9q34

NTNG2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
NTNG2 gene location

GeneLoc information about chromosome 9         GeneLoc Exon Structure

GeneLoc location for GC09P135037:  view genomic region     (about GC identifiers)

Start:
135,037,334 bp from pter      End:
135,119,921 bp from pter
Size:
82,588 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: NTNG2_HUMAN, Q96CW9 (See protein sequence)
Recommended Name: Netrin-G2 precursor  
Size: 530 amino acids; 59799 Da
Subunit: Interacts with LRRC4 (By similarity)
Subcellular location: Cell membrane; Lipid-anchor, GPI-anchor (By similarity)
Sequence caution: Sequence=BAB47486.2; Type=Erroneous initiation; Note=Translation N-terminally shortened;
3 PDB 3D structures from and Proteopedia for NTNG2:
3TBD (3D)        3ZYG (3D)        3ZYI (3D)    
Secondary accessions: Q5JUJ2 Q6UXY0 Q96JH0
Alternative splicing: 2 isoforms:  Q96CW9-1   Q96CW9-2   (No experimental confirmation available)

Explore the universe of human proteins at neXtProt for NTNG2: NX_Q96CW9

Post-translational modifications:

  • N-glycosylated (By similarity)1
  • View modification sites using PhosphoSitePlus2
  • View neXtProt modification sites for NX_Q96CW9

  • NTNG2 Protein expression data from MOPED and PaxDb:    About this image 
    Estimated protein expression log10 (pmol).

    REFSEQ proteins: NP_115925.2  
    ENSEMBL proteins: 
     ENSP00000376921   ENSP00000376920   ENSP00000353888   ENSP00000361252  

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    Browse Sino Biological Recombinant Proteins
    Browse ProSpec Recombinant Proteins
    Browse Proteins at Uscn

    Gene Ontology (GO): 2 cellular component terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005886plasma membrane ----
    GO:0046658anchored to plasma membrane ISS--


    NTNG2 for ontologies           About GeneDecksing



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    Browse ELISAs and CLIAs at Uscn


    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    NTNG2 for domains           About GeneDecksing

    5 InterPro domains/families:
     IPR000742 EG-like_dom
     IPR008211 Laminin_N
     IPR013032 EGF-like_CS
     IPR013111 EGF_extracell
     IPR002049 EGF_laminin

    Graphical View of Domain Structure for InterPro Entry Q96CW9

    ProtoNet protein and cluster: Q96CW9

    1 Blocks protein family: IPB002049 Laminin-type EGF-like domain

    UniProtKB/Swiss-Prot: NTNG2_HUMAN, Q96CW9
    Similarity: Contains 3 laminin EGF-like domains
    Similarity: Contains 1 laminin N-terminal domain


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section

    Function Summary:

         UniProtKB/Swiss-Prot: NTNG2_HUMAN, Q96CW9
    Function: Promotes neurite outgrowth of both axons and dendrites (By similarity)

    miRNA
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    Inhib. RNA
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    Gene Ontology (GO): 1 molecular function term (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003674molecular_function ND--


    NTNG2 for ontologies           About GeneDecksing


    1 GenomeRNAi human phenotype for NTNG2:
     Decreased viability with pacli 

    Animal Models:
         Mouse knock-outs for NTNG2: Ntng2tm1Ito Ntng2tm1Lex
         3 MGI mutant phenotypes (inferred from 2 alleles(MGI details for Ntng2):
     behavior/neurological  hearing/vestibular/ear  mortality/aging 

    NTNG2 for phenotypes           About GeneDecksing


    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section



    Interactions:

        Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for NTNG2

    STRING Interaction Network Preview (showing 2 interactants - click image to see more details)

    5/6 Interacting proteins for NTNG2 (Q96CW92, 3 ENSP000003769214) via UniProtKB, MINT, STRING, and/or I2D (see all 6)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    LRRC4Q9HBW12, ENSP000002493634MINT-8300800 MINT-8300766 MINT-8300722 MINT-8300489 STRING: ENSP00000249363
    EWSR1Q018443, ENSP000003810314I2D: score=3 STRING: ENSP00000381031
    LRRC4CQ9HCJ22MINT-8300885 MINT-8300842
    SHCBP1Q8NEM23I2D: score=1 
    --Q96MX43I2D: score=1 
    About this table

    Gene Ontology (GO): 1 biological process term (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0007409axonogenesis ISS--


    NTNG2 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section

    NTNG2 for compounds           About GeneDecksing

    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for NTNG2
    1 Novoseek chemical compound relationship for NTNG2 gene    About this table
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    nmda 39.6 1 16502428 (1)

    Search CenterWatch for drugs/clinical trials and news about NTNG2 

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section

    REFSEQ mRNAs for NTNG2 gene: 
    NM_032536.2  

    Unigene Cluster for NTNG2:

    Netrin G2
    Hs.163642  [show with all ESTs]
    Unigene Representative Sequence: AY358165
    7 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000393229(uc004cbh.2) ENST00000467453 ENST00000393228 ENST00000490694
    ENST00000483055 ENST00000360670 ENST00000372179

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    hsa-miR-148b* hsa-miR-3168 hsa-miR-629* hsa-miR-2117
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    Inhib. RNA
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      QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat NTNG2
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    Additional cDNA sequence: 

    AB058760.2 AF131842.1 AY358165.1 BC013770.1 

    4 DOTS entries:

    DT.75141862  DT.100747266  DT.97835561  DT.97813455 

    24/37 AceView cDNA sequences (see all 37):

    BX281558 BM126382 BM930930 BF930108 BQ894522 BX099434 BX279957 AB058760 
    BC013770 T11457 BU159259 AY358165 BM547925 BM053055 NM_032536 CK903969 
    AF131842 BI031197 BM684442 H83981 BF338562 CA393159 BM932044 H83980 

    GeneLoc Exon Structure


    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    NTNG2 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS
    CGAP TAG: GCTCTCCCAA

    Microarray
    RNAseq (Illumina Body Map)
    (100×FPKM)½
    SAGE (Serial Analysis of Gene Expression)

    About this image

    NTNG2 expression in embryonic tissues and stem cells
    Expression by the Database of Embryonic development, Stem cell research, and Regenerative medicine    About this table
    7 LifeMap In Vivo Development Anatomical Compartments/Cells 
    Tissue Anatomical Compartment CellCategory (developmental path)
    BrainHypothalamusBrain
    BrainMedulla OblongataBrain
    BrainThalamusBrain
    KidneyGlomerulusKidney
    Neural TubeDiencephalonNeural Tube
    Neural TubeMetencephalonNeural Tube
    Neural TubeTelencephalonNeural Tube
    Expression: Positive    Negative     Selective marker
    Experimental details: Curated     Microarrays     In-situ hybridization

    See NTNG2 Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for NTNG2

    SOURCE GeneReport for Unigene cluster: Hs.163642
        SABiosciences Custom PCR Arrays for NTNG2
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    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for NTNG2

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the common ancestor of chordates.

    Orthologs for NTNG2 gene from 3/13 species (see all 13)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    chicken
    (Gallus gallus)
    Aves NTNG21 netrin G2 81.02(n)
    83.59(a)
      417171  XM_415454.2  XP_415454.2 
    African clawed frog
    (Xenopus laevis)
    Amphibia Xl.87292 Xenopus laevis transcribed sequence with weak similarity more 81.53(n)    AW159335.1 
    zebrafish
    (Danio rerio)
    Actinopterygii ntng2a1 netrin g2a 69.26(n)
    69.13(a)
      560014  XM_683405.2  XP_688497.2 


    ENSEMBL Gene Tree for NTNG2 (if available)
    TreeFam Gene Tree for NTNG2 (if available) 

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    Paralogs for NTNG2 gene
    NTN42  LAMA12  NTN52  LAMA32  LAMA22  LAMB22  LAMC12  LAMC22  
    LAMA52  NTN32  LAMB32  LAMC32  LAMA42  NTN12  NTNG12  USH2A2  
    LAMB42  LAMB12  
    1 SIMAP similar gene for NTNG2 using alignment to 3 protein entries:     NTNG2_HUMAN (see all proteins):
    NTNG1

    NTNG2 for paralogs           About GeneDecksing



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/1504 NCBI SNPs in NTNG2 are shown (see all 1504    About this table
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 9 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs112436491,2
    F,H,--135035376(+) GCTGAG/ATGCTT 1 -- us2k15Minor allele frequency- A:0.24NS EA WA 496
    rs112436501,2
    C,F,H,--135035602(+) TCTCAC/TATATG 1 -- us2k111Minor allele frequency- T:0.05NS EA NA CSA WA 1206
    rs735592721,2
    C,--135035637(+) TGGGCG/CGTGTT 1 -- us2k11Minor allele frequency- C:0.50WA 2
    rs1886103481,2
    --135035710(+) TCCCCA/GGCCAC 1 -- us2k10--------
    rs2016882131,2
    --135035874(+) AAATA-/TATTATTA 1 -- us2k10--------
    rs1927167511,2
    --135035971(+) ACTCCA/GTCTCC 1 -- us2k10--------
    rs795417141,2
    F,--135035994(+) AGCTGA/GGAACA 1 -- us2k11Minor allele frequency- G:0.02NA 120
    rs665800361,2
    C,F,--135036019(+) TGGAGG/ATGAAG 1 -- us2k16Minor allele frequency- A:0.15NA CSA WA EA 364
    rs1507674661,2
    --135036050(+) CTGAGA/GTGCCA 1 -- us2k10--------
    rs787651261,2
    --135036320(+) TGCCGG/AATATG 1 -- us2k11Minor allele frequency- A:0.01WA 118

    HapMap Linkage Disequilibrium report for NTNG2 (135037334 - 135119921 bp)
    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
    Database of Genomic Variants (DGV): 11 variations for NTNG2
         11 Indels: 83204 60771 96327 83206 83203 96326 96325 96324 83202 83205 96323

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    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    NTNG2 for disorders           About GeneDecksing

    6 diseases for NTNG2:    About MalaCards
    systemic lupus erythematosus    lupus erythematosus    rett syndrome    bipolar disorder
    schizophrenia    neuronitis

    1 disease from the University of Copenhagen DISEASES database for NTNG2:
    Rett syndrome
    Human Genome Epidemiology (HuGE) Navigator: NTNG2 (1 document)

    Export disorders for NTNG2 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for NTNG2 gene, integrated from 9 sources (see all 24):
    (articles sorted by number of sources associating them with NTNG2)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
    2. The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment. (PubMed id 12975309)1, 2 Clark H.F.... Gray A.M. (2003)
    3. Construction of expression-ready cDNA clones for KIAA genes: manual curation of 330 KIAA cDNA clones. (PubMed id 12168954)1, 2 Nakajima D.... Nagase T. (2002)
    4. Prediction of the coding sequences of unidentified human genes. XX. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. (PubMed id 11347906)1, 2 Nagase T.... Ohara O. (2001)
    5. Decreased mRNA expression of netrin-G1 and netrin-G2 in the temporal lobe in schizophrenia and bipolar disorder. (PubMed id 17507910)1, 9 Eastwood S.L. and Harrison P.J. (2008)
    6. A family-based association study and gene expression analyses of netrin-G1 and -G2 genes in schizophrenia. (PubMed id 15705354)1, 9 Aoki-Suzuki M....Yoshikawa T. (2005)
    7. Topography of Gng2- and NetrinG2-expression suggests a n insular origin of the human claustrum. (PubMed id 22957104)1 Pirone A....Castagna M. (2012)
    8. Crystal structure of the ligand binding domain of net rin G2. (PubMed id 22041449)1 Brasch J....Shapiro L. (2011)
    9. Abnormal expression of netrin-G2 in temporal lobe epi lepsy neurons in humans and a rat model. (PubMed id 20382146)1 Pan Y....Wang X. (2010)
    10. Association of polymorphisms in the SLIT2 axonal guid ance gene with anger in suicide attempters. (PubMed id 20029409)1 Sokolowski M....Wasserman D. (2010)

    (in PubMed, OMIM, and NCBI Bookshelf)
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    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 84628 HGNC: 14288 AceView: NTNG2 Ensembl:ENSG00000196358 euGenes: HUgn84628
    ECgene: NTNG2 H-InvDB: NTNG2

    (According to HUGE)
    About This Section
    HUGE: KIAA1857

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for NTNG2 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section
    Patent Information for NTNG2 gene:
    Search GeneIP for patents involving NTNG2

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



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