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NT5E Gene

protein-coding   GIFtS: 71
GCID: GC06P086216

5'-Nucleotidase, Ecto (CD73)

(Previous name: 5' nucleotidase (CD73))
(Previous symbol: NT5)
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(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
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Aliases
5'-Nucleotidase, Ecto (CD73)1 2     E5NT2
NT51 2 3 5     NT2
5'-NT2 3     eN2
NTE2 3     eNT2
EC 3.1.3.53 8     ecto-5'-nucleotidase2
5' Nucleotidase (CD73)1     Purine 5-Prime-Nucleotidase2
5'-nucleotidase2     Ecto-5'-nucleotidase3
CALJA2     CD73 Antigen3
CD732     

External Ids:    HGNC: 80211   Entrez Gene: 49072   Ensembl: ENSG000001353187   OMIM: 1291905   UniProtKB: P215893   

Export aliases for NT5E gene to outside databases

Previous GC identifers: GC06P086106 GC06P086155 GC06P083383


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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Entrez Gene summary for NT5E Gene:
The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular
nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte
differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript
variants encoding different isoforms have been found for this gene.(provided by RefSeq, Mar 2011)

GeneCards Summary for NT5E Gene:
NT5E (5'-nucleotidase, ecto (CD73)) is a protein-coding gene. Diseases associated with NT5E include calcification of joints and arteries, and estrogen-receptor negative breast cancer. GO annotations related to this gene include nucleotide binding.

UniProtKB/Swiss-Prot: 5NTD_HUMAN, P21589
Function: Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Exhibits AMP-, NAD-, and
NMN-nucleosidase activities

Gene Wiki entry for NT5E Gene


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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RefSeq DNA sequence:
NC_000006.12  NC_018917.2  NT_025741.16  
Regulatory elements:
   Regulatory transcription factor binding sites in the NT5E gene promoter:
         GR   AP-1   GR-beta   ATF-2   SREBP-1c   SREBP-1b   SREBP-1a   c-Jun   GR-alpha   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidNT5E promoter sequence
   Search Chromatin IP Primers for NT5E

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat NT5E


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 6q14-q21   Ensembl cytogenetic band:  6q14.3   HGNC cytogenetic band: 6q14-q21

NT5E Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
NT5E gene location

GeneLoc information about chromosome 6         GeneLoc Exon Structure

GeneLoc location for GC06P086216:  view genomic region     (about GC identifiers)

Start:
86,159,302 bp from pter      End:
86,205,509 bp from pter
Size:
46,208 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Cloud-Clone Corp.,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Cloud-Clone Corp., Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, and/or Cloud-Clone Corp.)
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UniProtKB/Swiss-Prot: 5NTD_HUMAN, P21589 (See protein sequence)
Recommended Name: 5'-nucleotidase precursor  
Size: 574 amino acids; 63368 Da
Cofactor: Zinc
Subunit: Homodimer
6 PDB 3D structures from and Proteopedia for NT5E:
4H1S (3D)        4H1Y (3D)        4H2B (3D)        4H2F (3D)        4H2G (3D)        4H2I (3D)    
Secondary accessions: B3KQI8 O75520 Q5W116
Alternative splicing: 2 isoforms:  P21589-1   P21589-2   (No experimental confirmation available)

Explore the universe of human proteins at neXtProt for NT5E: NX_P21589

Explore proteomics data for NT5E at MOPED

Post-translational modifications: 

  • Glycosylation2 at Asn53, Asn311, Asn333, Asn403
  • Modification sites at PhosphoSitePlus
  • Selected DME Specific Peptides for NT5E (P21589) (see all 10)
     YISKMKV  TGEFLQV  GNPILLNS  YPAVEGRI 


    See NT5E Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins (2 alternative transcripts): 
    NP_001191742.1  NP_002517.1  

    ENSEMBL proteins: 
     ENSP00000358660   ENSP00000257770   ENSP00000414674   ENSP00000387630   ENSP00000358665  
    Reactome Protein details: P21589

    NT5E Human Recombinant Protein Products:

    Browse Purified and Recombinant Proteins at EMD Millipore
    R&D Systems Recombinant & Natural Proteins for NT5E (5'-Nucleotidase/CD73)
    Enzo Life Sciences proteins for NT5E
    Browse OriGene full length recombinant human proteins expressed in human HEK293 cells
    OriGene Protein Over-expression Lysate for NT5E
    OriGene Custom MassSpec
    OriGene Custom Protein Services for NT5E
    GenScript Custom Purified and Recombinant Proteins Services for NT5E
    Novus Biologicals NT5E Protein
    Novus Biologicals NT5E Lysate
    Sino Biological Recombinant Protein for NT5E
    Sino Biological Cell Lysate for NT5E
    Browse ProSpec Recombinant Proteins
    Cloud-Clone Corp. Proteins for NT5E

    NT5E Antibody Products:

    EMD Millipore Mono- and Polyclonal Antibodies for the study of NT5E
    R&D Systems Antibodies for NT5E (5'-Nucleotidase/CD73)
    Cell Signaling Technology (CST) Antibodies for NT5E 
    OriGene Antibodies for NT5E
    OriGene Custom Antibody Services for NT5E
    Novus Biologicals NT5E Antibodies
    Abcam antibodies for NT5E
    Cloud-Clone Corp. Antibodies for NT5E
    ThermoFisher Antibodies for NT5E
    LSBio Antibodies in human, mouse, rat for NT5E

    NT5E Assay Products:

    Browse Kits and Assays available from EMD Millipore
    OriGene Custom Assay Services for NT5E
    Browse R&D Systems for biochemical assays
    GenScript Custom Assay Services for NT5E
    Browse Enzo Life Sciences for kits & assays
    Cloud-Clone Corp. ELISAs for NT5E
    Cloud-Clone Corp. CLIAs for NT5E


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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    HGNC Gene Families:
    CD: CD molecules

    IUPHAR Guide to PHARMACOLOGY protein family classification: Ecto-5'-Nucleotidase
    Adenosine turnover

    4 InterPro protein domains:
     IPR004843 PEstase_dom
     IPR006146 5'-Nucleotdase_CS
     IPR008334 5'-Nucleotdase_C
     IPR006179 5_nucleotidase/apyrase

    Graphical View of Domain Structure for InterPro Entry P21589

    ProtoNet protein and cluster: P21589

    2 Blocks protein domains:
    IPB006179 Apyrase family signature
    IPB008334 -Nucleotdase_C


    UniProtKB/Swiss-Prot: 5NTD_HUMAN, P21589
    Similarity: Belongs to the 5'-nucleotidase family


    NT5E for domains           About GeneDecksing


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Molecular Function:

         UniProtKB/Swiss-Prot Summary: 5NTD_HUMAN, P21589
    Function: Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Exhibits AMP-, NAD-, and
    NMN-nucleosidase activities
    Catalytic activity: A 5'-ribonucleotide + H(2)O = a ribonucleoside + phosphate

         Enzyme Number (IUBMB): EC 3.1.3.51 2

         Gene Ontology (GO): 5 molecular function terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000166nucleotide binding IEA--
    GO:00082535'-nucleotidase activity EXP--
    GO:0016787hydrolase activity ----
    GO:0016788hydrolase activity, acting on ester bonds ----
    GO:0046872metal ion binding IEA--
         
    NT5E for ontologies           About GeneDecksing


    Phenotypes:
         2 GenomeRNAi human phenotypes for NT5E:
     Decreased melanin production  Increased G2M DNA content 

         5 MGI mutant phenotypes (inferred from 3 alleles(MGI details for Nt5e):
     behavior/neurological  cardiovascular system  hematopoietic system  homeostasis/metabolism  renal/urinary system 

    NT5E for phenotypes           About GeneDecksing

    Animal Models:
         MGI mouse knock-outs for NT5E: Nt5etm1Lft Nt5etm1Jsc Nt5etm1Jgsc

       inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for NT5E
       inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for NT5E

       genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for NT5E
       genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for NT5E

    miRNA
    Products:
        
    miRTarBase miRNAs that target NT5E:
    hsa-mir-124-3p (MIRT022996), hsa-mir-155-5p (MIRT001529), hsa-mir-34a-5p (MIRT025509), hsa-mir-1 (MIRT023955), hsa-mir-30a-5p (MIRT005130)

    Block miRNA regulation of human, mouse, rat NT5E using miScript Target Protectors
    Selected qRT-PCR Assays for microRNAs that regulate NT5E (see all 19):
    hsa-miR-411* hsa-miR-30c hsa-miR-193a-3p hsa-miR-379* hsa-miR-657 hsa-miR-578 hsa-miR-30d hsa-miR-4260
    SwitchGear 3'UTR luciferase reporter plasmidNT5E 3' UTR sequence
    Inhib. RNA
    Products:
        
    OriGene RNAi products in human, mouse, rat for NT5E
    Predesigned siRNA for gene silencing in human, mouse, rat NT5E

    Gene Editing
    Products:
    DNA2.0 Custom Protein Engineering Service for NT5E

    Clone
    Products:
         
    OriGene clones in human, mouse for NT5E (see all 7)
    OriGene ORF clones in mouse, rat for NT5E
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector (see all 2): NT5E (NM_002526)
    Sino Biological Human cDNA Clone for NT5E
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for NT5E
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat NT5E

    Cell Line
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    GenScript Custom overexpressing Cell Line Services for NT5E
    Browse ESI BIO Cell Lines and PureStem Progenitors for NT5E 
    In Situ Assay
    Products:
       

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for NT5E


    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Subcellular locations from UniProtKB/Swiss-Prot
    5NTD_HUMAN, P21589: Cell membrane; Lipid-anchor, GPI-anchor
    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    extracellular5
    plasma membrane5
    cytosol3
    endoplasmic reticulum3
    mitochondrion3
    golgi apparatus2
    lysosome2
    nucleus2
    vacuole2
    cytoskeleton1
    endosome1

    Gene Ontology (GO): 5 cellular component terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005737cytoplasm IDA--
    GO:0005886plasma membrane TAS--
    GO:0016020membrane TAS2129526
    GO:0031225anchored component of membrane IEA--
    GO:0070062extracellular vesicular exosome IDA19056867

    NT5E for ontologies           About GeneDecksing


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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    SuperPaths for NT5E About   (see all 9)  
    See pathways by source

    SuperPathContained pathways About
    1Purine metabolism (REACTOME)
    Purine metabolism0.42
    Purine catabolism0.00
    Metabolism of nucleotides0.42
    urate biosynthesis/inosine 5-phosphate degradation0.00
    purine nucleotides degradation0.00
    adenosine nucleotides degradation0.00
    2Purine metabolism (KEGG)
    Purine metabolism0.38
    ATP ITP metabolism0.31
    Pyrimidine metabolism0.38
    3Nicotinate and nicotinamide metabolism
    Nicotinate and nicotinamide metabolism0.43
    NAD metabolism0.43
    4Metabolism
    Metabolism0.38
    Metabolic pathways0.38
    5Pyrimidine metabolism
    Pyrimidine metabolism0.33
    Pyrimidine catabolism0.00

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways


    2 Cell Signaling Technology (CST) Pathways for NT5E
        Neuroscience
    Lymphocyte Signaling

    2 GeneGo (Thomson Reuters) Pathways for NT5E
        NAD metabolism
    ATP/ITP metabolism

    4 BioSystems Pathways for NT5E
        HIF-1-alpha transcription factor network
    adenosine nucleotides degradation
    urate biosynthesis/inosine 5-phosphate degradation
    purine nucleotides degradation


    2 Reactome Pathways for NT5E
        Pyrimidine catabolism
    Purine catabolism

    1 PharmGKB Pathway for NT5E
        Thiopurine Pathway, Pharmacokinetics/Pharmacodynamics

    4 Kegg Pathways  (Kegg details for NT5E):
        Purine metabolism
    Pyrimidine metabolism
    Nicotinate and nicotinamide metabolism
    Metabolic pathways


    NT5E for pathways           About GeneDecksing

        Pathway & Disease-focused RT2 Profiler PCR Arrays including NT5E: 
              Cell Surface Markers in human mouse rat
              Mesenchymal Stem Cell in human mouse rat

    Interactions:

        GeneGlobe Interaction Network for NT5E

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    Selected Interacting proteins for NT5E (P215893 ENSP000002577704) via UniProtKB, MINT, STRING, and/or I2D (see all 89)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    ACTBP607093, ENSP000003499604I2D: score=1 STRING: ENSP00000349960
    FN1P027513, ENSP000003468394I2D: score=1 STRING: ENSP00000346839
    LAMA1P253913, ENSP000003743094I2D: score=1 STRING: ENSP00000374309
    AFF1P518253, ENSP000003056894I2D: score=1 STRING: ENSP00000305689
    ADAENSP000003619654STRING: ENSP00000361965
    About this table

    Gene Ontology (GO): Selected biological process terms (see all 12):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006144purine nucleobase metabolic process TAS--
    GO:0006195purine nucleotide catabolic process TAS--
    GO:0006196AMP catabolic process IEA--
    GO:0006206pyrimidine nucleobase metabolic process TAS--
    GO:0006259DNA metabolic process TAS2129526

    NT5E for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
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    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
      Browse compounds at ApexBio 

    Browse Tocris compounds for NT5E (5NTD)

    2 DrugBank Compounds for NT5E    About this table
    CompoundSynonyms CAS #TypeActionsPubMed Ids
    Pentoxifylline-- 6493-05-6targetinhibitor11752352 16426349
    CytarabineAra-C (see all 26)147-94-4enzymesubstrate19842938

    1 IUPHAR Ligand for NT5E (Ecto-5'-Nucleotidase)    About this table
    LigandTypeActionAffinityPubmed IDs
    alphabeta-methyleneADP
    InhibitorInhibition8.71169962

    Selected Novoseek inferred chemical compound relationships for NT5E gene (see all 24)    About this table
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    apcp 87.9 4 17487388 (2), 16644754 (1), 17671792 (1)
    epsilon-amp 86.8 5 16418778 (1), 17065216 (1), 11046060 (1), 17471030 (1)
    alpha,beta-methyleneadenosine-5'-diphosphate 83.6 1 17487388 (1)
    purine 57 13 8566797 (2), 8906810 (2), 19253308 (2), 8224905 (1) (see all 8)
    cyclic adp-ribose 42.9 7 15028729 (3), 8906810 (1)
    nucleoside 39.9 9 16644754 (1), 18600539 (1), 16265592 (1), 15028729 (1) (see all 6)
    adenylate 38.3 12 19729987 (2), 9169488 (2), 11046060 (2), 16861292 (1) (see all 7)
    purine nucleoside 36.3 2 7655619 (1), 10343529 (1)
    atp 34.3 13 19729987 (2), 19464286 (1), 19825957 (1), 16799190 (1) (see all 10)
    adp 21.5 3 19464286 (1), 15319286 (1)



    NT5E for compounds           About GeneDecksing



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    Conferences by KenesGroup, exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, and/or QIAGEN )
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    REFSEQ mRNAs for NT5E gene (2 alternative transcripts): 
    NM_001204813.1  NM_002526.3  

    Unigene Cluster for NT5E:

    5'-nucleotidase, ecto (CD73)
    Hs.153952  [show with all ESTs]
    Unigene Representative Sequence: NM_002526
    5 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000369646(uc003pkn.3) ENST00000257770(uc003pko.4 uc010kbr.3)
    ENST00000416334 ENST00000437581 ENST00000369651

    miRNA
    Products:
         
    Block miRNA regulation of human, mouse, rat NT5E using miScript Target Protectors
    Selected qRT-PCR Assays for microRNAs that regulate NT5E (see all 19):
    hsa-miR-411* hsa-miR-30c hsa-miR-193a-3p hsa-miR-379* hsa-miR-657 hsa-miR-578 hsa-miR-30d hsa-miR-4260
    SwitchGear 3'UTR luciferase reporter plasmidNT5E 3' UTR sequence
    Inhib. RNA
    Products:
         
    OriGene RNAi products in human, mouse, rat for NT5E
    Predesigned siRNA for gene silencing in human, mouse, rat NT5E
    Clone
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    OriGene clones in human, mouse for NT5E (see all 7)
    OriGene ORF clones in mouse, rat for NT5E
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector (see all 2): NT5E (NM_002526)
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for NT5E
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat NT5E
    Primer
    Products:
        
    OriGene qPCR primer pairs and template standards for NT5E
    OriGene qSTAR qPCR primer pairs in human, mouse for NT5E
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat NT5E
      QuantiTect SYBR Green Assays in human, mouse, rat NT5E
      QuantiFast Probe-based Assays in human, mouse, rat NT5E

    Additional mRNA sequence: 

    AK075008.1 AK075083.1 AK314661.1 BC015940.1 BC065937.1 DQ186653.1 X55740.1 

    14 DOTS entries:

    DT.100819418  DT.101954345  DT.100819415  DT.100819421  DT.213229  DT.100819414  DT.100728243  DT.121330217 
    DT.121330067  DT.115575  DT.100819416  DT.97806655  DT.100676979  DT.121330062 

    Selected AceView cDNA sequences (see all 269):

    BP364913 AI935864 BM847311 AW271686 BM761656 AI420969 BU626182 F01662 
    CF131258 CB132912 AA910593 CA487257 F03087 BF476532 CN485065 W51864 
    AA331558 F09560 H98118 F11913 BP341857 BE876521 W24986 AI989471 

    GeneLoc Exon Structure

    3 Alternative Splicing Database (ASD) splice patterns (SP) for NT5E    About this scheme

    ExUns: 1a · 1b ^ 2 ^ 3a · 3b ^ 4 ^ 5 ^ 6 ^ 7 ^ 8 ^ 9
    SP1:                          -                                       
    SP2:                          -                       -               
    SP3:                                                                  


    ECgene alternative splicing isoforms for NT5E

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

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    NT5E expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: AGCCTGCTCA
    NT5E Expression
    About this image


    NT5E expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database
     selected tissues (see all 26) fully expand
     
     Mesenchymal Stem Cells
             Bone Marrow Stromal Cells Bone Marrow
             Decidua-derived mesenchymal stem cells
             Myofibroblasts/mesenchymal-like cells
     
     Bone (Muscoskeletal System)    fully expand to see all 21 entries
             Bone Marrow Stromal Cells Bone Marrow
             Bone marrow-derived mesenchymal stem cells (HYNR-CS inj)
     
     Tooth (Integumentary System)    fully expand to see all 11 entries
             Dental Pulp Stem Cells Dental Pulp
             Stem cells from human exfoliated deciduous teeth
     
     Umbilical Cord (Extraembryonic Tissues)    fully expand to see all 9 entries
             Mesenchymal Stem Cells Wharton's Jelly
             Umbilical tissue-derived cells (CNTO2476)
     
     Placenta (Extraembryonic Tissues)    fully expand to see all 8 entries
             Chorionic Mesenchymal Stromal Cells Chorionic Villus
             Decidua-derived mesenchymal stem cells
    NT5E Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    NT5E Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.153952
        Pathway & Disease-focused RT2 Profiler PCR Arrays including NT5E: 
              Cell Surface Markers in human mouse rat
              Mesenchymal Stem Cell in human mouse rat

    Primer
    Products:
    OriGene qPCR primer pairs and template standards for NT5E
    OriGene qSTAR qPCR primer pairs in human, mouse for NT5E
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat NT5E
    QuantiTect SYBR Green Assays in human, mouse, rat NT5E
    QuantiFast Probe-based Assays in human, mouse, rat NT5E
    In Situ
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    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
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    This gene was present in the common ancestor of animals.

    Orthologs for NT5E gene from Selected species (see all 16)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Nt5e1 , 5 5' nucleotidase, ecto1, 5 85.42(n)1
    86.76(a)1
      9 (47.24 cM)5
    239591  NM_011851.41  NP_035981.11 
     883276095 
    chicken
    (Gallus gallus)
    Aves NT5E1 5'-nucleotidase, ecto (CD73) 65.75(n)
    65.01(a)
      421833  XM_004940397.1  XP_004940454.1 
    lizard
    (Anolis carolinensis)
    Reptilia --
    --
    Uncharacterized protein
    62(a)
    55(a)
    many → 1
    many → 1
    1(176888687-176906681)
    1(196518684-196533404)
    African clawed frog
    (Xenopus laevis)
    Amphibia Xl.144552 Xenopus laevis transcribed sequence with moderate similarity more 76.69(n)    BJ042561.1 
    zebrafish
    (Danio rerio)
    Actinopterygii zgc637842 similar to 5' nucleotidase, ecto 76.13(n)   393906  BC055243.1 
    fruit fly
    (Drosophila melanogaster)
    Insecta CG48273
    veil1
    5'-nucleotidase3
    veil1
    39(a)
    (best of 4)3
    48.99(n)1
    41.44(a)1
      54B173
    369681  NM_137373.21  NP_611217.11 


    ENSEMBL Gene Tree for NT5E (if available)
    TreeFam Gene Tree for NT5E (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
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    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
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    Selected SNPs for NT5E (see all 859)    About this table    
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 6 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    VAR_0651854
    Calcification of joints and arteries (CALJA)4--see VAR_0651852 C Y mis40--------
    rs1117134971,2
    F--86137144(+) AAAGCC/ACTGAT 2 -- us2k11Minor allele frequency- A:0.50CSA 2
    rs1465508841,2
    --86137153(+) ATTTGA/TATGTA 2 -- us2k10--------
    rs94502771,2
    C--86137228(+) TTGCAG/ACTACT 2 -- us2k15Minor allele frequency- A:0.35NA WA EA 362
    rs734962421,2
    C,F--86137317(+) TCGCCC/TACTGT 2 -- us2k12Minor allele frequency- T:0.08WA 120
    rs1162977161,2
    --86137324(+) CTGTGG/TCTAAA 2 -- us2k10--------
    rs1168745231,2
    F--86137468(+) AGTGAG/CATTGT 2 -- us2k11Minor allele frequency- C:0.02NA 120
    rs1827358421,2
    --86137492(+) AAATTC/TAGAAA 2 -- us2k10--------
    rs1159051751,2
    F--86137513(+) TCACTG/ACTGTG 2 -- us2k11Minor allele frequency- A:0.05WA 118
    rs1874274101,2
    --86137642(+) TGTGTA/GAGGAA 2 -- us2k10--------

    HapMap Linkage Disequilibrium report for NT5E (86159302 - 86205509 bp)

    Structural Variations
         Database of Genomic Variants (DGV) 4 variations for NT5E:    About this table    
    Variant IDTypeSubtypePubMed ID
    nsv886333CNV Loss21882294
    nsv470839CNV Loss18288195
    nsv520112CNV Loss19592680
    nsv463939CNV Loss19166990

    Human Gene Mutation Database (HGMD): NT5E
    Site Specific Mutation Identification with PCR Assays
    SeqTarget long-range PCR primers for resequencing NT5E
    DNA2.0 Custom Variant and Variant Library Synthesis for NT5E

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Conferences by KenesGroup, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
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    OMIM gene information: 129190   
    OMIM disorders: 211800  
    UniProtKB/Swiss-Prot: 5NTD_HUMAN, P21589
  • Calcification of joints and arteries (CALJA) [MIM:211800]: A condition characterized by adult-onset
    calcification of the lower extremity arteries, including the iliac, femoral and tibial arteries, and hand and
    foot capsule joints. Age of onset has been reported as early as the second decade of life, usually involving
    intense joint pain or calcification in the hands. Note=The disease is caused by mutations affecting the gene
    represented in this entry

  • Selected diseases for NT5E (see all 45):    
    About MalaCards
    calcification of joints and arteries    estrogen-receptor negative breast cancer    varicocele    periostitis
    agammaglobulinemia    portal hypertension    beta thalassemia    thalassemia
    chronic lymphocytic leukemia    b-cell chronic lymphocytic leukemia    papillary thyroid carcinoma    eclampsia
    cholestasis    pre-eclampsia    gastritis    gingivitis
    idiopathic pulmonary fibrosis    glioblastoma multiforme    aortic aneurysm    acute leukemia

    4 diseases from the University of Copenhagen DISEASES database for NT5E:
    Anemia     Vascular disease     Cholestasis     Leukemia

    NT5E for disorders           About GeneDecksing


    Congresses - knowledge worth sharing:
    The International Symposium on Pneumococci and Pneumococcal Diseases (ISPPD) 9 - 13 March 2014

    Selected Novoseek inferred disease relationships for NT5E gene (see all 12)    About this table

    Disease   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    leukemia t-cell 30.5 2 8027539 (1), 2070084 (1)
    zinc deficiency 20.4 3 1940572 (2), 9280142 (1)
    inflammation 15.8 4 14734746 (1), 17911479 (1), 19149741 (1)
    leukemia 0 8 2070084 (4), 8566797 (1)
    primary tumor 0 2 2183704 (1), 17065075 (1)
    breast cancer 0 18 17587186 (4), 17487388 (3), 17671792 (3), 17471030 (2) (see all 5)
    metastasis 0 5 2183704 (1), 17065075 (1), 17587186 (1), 17471030 (1)
    tumors 0 5 8566797 (2), 17487388 (2)
    lymphoma 0 2 8566797 (1)
    melanoma 0 5 17065075 (2), 16718268 (1)

    Genetic Association Database (GAD): NT5E
    Human Genome Epidemiology (HuGE) Navigator: NT5E (3 documents)

    Export disorders for NT5E gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
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    PubMed articles for NT5E gene, integrated from 10 sources (see all 301):
    (articles sorted by number of sources associating them with NT5E)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Isolation and characterization of the promoter of the human 5'- nucleotidase (CD73)-encoding gene. (PubMed id 8566797)1, 2, 9 Hansen K.R.... Thompson L.F. (Gene 1995)
    2. Crystal structure of the human ecto-5'-nucleotidase (CD73): insights into the regulation of purinergic signaling. (PubMed id 23142347)1, 2 Knapp K.... Strater N. (Structure 2012)
    3. The high-resolution crystal structure of periplasmic Haemophilus influenzae NAD nucleotidase reveals a novel enzymatic function of human CD73 related to NAD metabolism. (PubMed id 21933152)1, 2 Garavaglia S.... Rizzi M. (Biochem. J. 2012)
    4. NT5E mutations and arterial calcifications. (PubMed id 21288095)1, 2 St Hilaire C.... Boehm M. (N. Engl. J. Med. 2011)
    5. Contribution of adenosine related genes to the risk of depression with disturbed sleep. (PubMed id 20392501)1, 4 Gass N....Paunio T. (J Affect Disord 2010)
    6. Evaluation of candidate stromal epithelial cross-talk genes identifies association between risk of serous ovarian cancer and TERT, a cancer susceptibility &quot;hot-spot&quot;. (PubMed id 20628624)1, 4 Johnatty S.E.... . (PLoS Genet. 2010)
    7. Explorative study to identify novel candidate genes related to oxaliplatin efficacy and toxicity using a DNA repair array. (PubMed id 19536092)1, 4 Kweekel D.M....Guchelaar H.J. (Br. J. Cancer 2009)
    8. Signal sequence and keyword trap in silico for selection of full- length human cDNAs encoding secretion or membrane proteins from oligo- capped cDNA libraries. (PubMed id 16303743)1, 2 Otsuki T....Isogai T. (DNA Res. 2005)
    9. Primary structure of human placental 5'-nucleotidase and identification of the glycolipid anchor in the mature form. (PubMed id 2129526)1, 2 Misumi Y.... Ikehara Y. (Eur. J. Biochem. 1990)
    10. Characterization of soluble vs membrane-bound human placental 5'- nucleotidase. (PubMed id 2173922)1, 2 Klemens M.R....Thompson L.F. (Biochem. Biophys. Res. Commun. 1990)

    (in PubMed, OMIM, and NCBI Bookshelf)
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     ANDOR
    Aliases
    Disorders
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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    Entrez Gene: 4907 HGNC: 8021 AceView: NT5E Ensembl:ENSG00000135318 euGenes: HUgn4907
    ECgene: NT5E Kegg: 4907 H-InvDB: NT5E

    (According to HUGE)
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    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
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    NameDescription
    PharmGKB entry for NT5E Pharmacogenomics, SNPs, Pathways
    ATLAS Chromosomes in Cancer entry for NT5E Genetics and Cytogenetics in Oncology and Haematology

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
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    Patent Information for NT5E gene:
    Search GeneIP for patents involving NT5E

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



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