Free for academic non-profit institutions. Other users need a Commercial license

Aliases for NT5E Gene

Aliases for NT5E Gene

  • 5'-Nucleotidase Ecto 2
  • 5-Nucleotidase Ecto 2 3 5
  • Ecto-5-Nucleotidase 3 4
  • EC 4 58
  • 5-NT 3 4
  • NT5 3 4
  • NTE 3 4
  • 5-Nucleotidase, Ecto (CD73) 2
  • Purine 5-Prime-Nucleotidase 3
  • 5 Nucleotidase (CD73) 2
  • 5-Nucleotidase 3
  • CD73 Antigen 4
  • CALJA 3
  • CD73 3
  • E5NT 3
  • ENT 3
  • NT 3
  • EN 3

External Ids for NT5E Gene

Previous HGNC Symbols for NT5E Gene

  • NT5

Previous GeneCards Identifiers for NT5E Gene

  • GC06P086106
  • GC06P086155
  • GC06P086216
  • GC06P083383

Summaries for NT5E Gene

Entrez Gene Summary for NT5E Gene

  • The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011]

CIViC summary for NT5E Gene

GeneCards Summary for NT5E Gene

NT5E (5'-Nucleotidase Ecto) is a Protein Coding gene. Diseases associated with NT5E include Calcification Of Joints And Arteries and Intestinal Tuberculosis. Among its related pathways are Metabolism and Purine metabolism. GO annotations related to this gene include nucleotide binding and hydrolase activity, acting on ester bonds.

UniProtKB/Swiss-Prot for NT5E Gene

  • Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Exhibits AMP-, NAD-, and NMN-nucleosidase activities.

Gene Wiki entry for NT5E Gene

Additional gene information for NT5E Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for NT5E Gene

Genomics for NT5E Gene

Regulatory Elements for NT5E Gene

Enhancers for NT5E Gene
GeneHancer Identifier Enhancer Score Enhancer Sources Gene-Enhancer Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor Binding Sites within enhancer Gene Targets for Enhancer
GH06H085456 1.8 FANTOM5 Ensembl ENCODE dbSUPER 32.9 +14.0 13987 14.9 PKNOX1 FOXA2 FOS ATF7 RUNX3 NFIL3 MAFF RCOR2 MYNN STAT3 SNHG5 NT5E PKMP3 GC06P085489
GH06H085401 1.9 FANTOM5 Ensembl ENCODE dbSUPER 23 -45.0 -44985 6.6 PKNOX1 FOXA2 MLX DMAP1 YY1 FOS REST PPARG KAT8 NFIL3 NT5E LINC02535 SNHG5 DUTP5
GH06H085447 1.3 ENCODE dbSUPER 33 +1.2 1233 6.2 FOXA2 ATF1 MLX ARID4B SIN3A DMAP1 ZNF2 YY1 ATF7 RUNX3 SNHG5 NT5E PKMP3 GC06P085489
GH06H085481 1.3 Ensembl ENCODE dbSUPER 24.5 +33.3 33342 2.3 ELF3 PKNOX1 RB1 TBL1XR1 ZNF2 RAD21 ZNF366 SCRT2 ZNF143 DEK NT5E GC06P085489
GH06H085435 0.8 ENCODE dbSUPER 33.5 -13.1 -13081 2.2 SCRT1 ZNF740 SCRT2 ZNF175 GTF2A2 ZNF7 NT5E SNHG5 TPT1P6
- Elite enhancer and/or Elite enhancer-gene association Download GeneHancer data dump

Enhancers around NT5E on UCSC Golden Path with GeneCards custom track

Promoters for NT5E Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters
ENSR00000199700 1016 3201 FOXA2 ATF1 MLX ARID4B SIN3A DMAP1 ZNF2 YY1 ATF7 RUNX3

Genomic Locations for NT5E Gene

Genomic Locations for NT5E Gene
46,208 bases
Plus strand

Genomic View for NT5E Gene

Genes around NT5E on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
NT5E Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for NT5E Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for NT5E Gene

Proteins for NT5E Gene

  • Protein details for NT5E Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Recommended name:
    Protein Accession:
    Secondary Accessions:
    • B3KQI8
    • O75520
    • Q5W116

    Protein attributes for NT5E Gene

    574 amino acids
    Molecular mass:
    63368 Da
    Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
    Quaternary structure:
    • Homodimer.

    Three dimensional structures from OCA and Proteopedia for NT5E Gene

    Alternative splice isoforms for NT5E Gene


neXtProt entry for NT5E Gene

Selected DME Specific Peptides for NT5E Gene


Post-translational modifications for NT5E Gene

  • Glycosylation at posLast=5353, Asn311, posLast=333333, and Asn403
  • Modification sites at PhosphoSitePlus

Other Protein References for NT5E Gene

Antibody Products

  • R&D Systems Antibodies for NT5E (5'-Nucleotidase/CD73)
  • Cell Signaling Technology (CST) Antibodies for NT5E (NT5E)
  • Abcam antibodies for NT5E

Protein Products

  • Abcam proteins for NT5E

Domains & Families for NT5E Gene

Gene Families for NT5E Gene

Human Protein Atlas (HPA):
  • CD markers
  • Disease related genes
  • Enzymes
  • Potential drug targets
  • Predicted intracellular proteins
  • Predicted membrane proteins
  • Predicted secreted proteins

Graphical View of Domain Structure for InterPro Entry



  • Belongs to the 5-nucleotidase family.
  • Belongs to the 5-nucleotidase family.
genes like me logo Genes that share domains with NT5E: view

Function for NT5E Gene

Molecular function for NT5E Gene

UniProtKB/Swiss-Prot CatalyticActivity:
A 5-ribonucleotide + H(2)O = a ribonucleoside + phosphate.
UniProtKB/Swiss-Prot Function:
Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Exhibits AMP-, NAD-, and NMN-nucleosidase activities.

Enzyme Numbers (IUBMB) for NT5E Gene

Phenotypes From GWAS Catalog for NT5E Gene

Gene Ontology (GO) - Molecular Function for NT5E Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000166 nucleotide binding IEA --
GO:0008253 5-nucleotidase activity IDA 7595232
GO:0016787 hydrolase activity IEA --
GO:0016788 hydrolase activity, acting on ester bonds IEA --
GO:0046872 metal ion binding IEA --
genes like me logo Genes that share ontologies with NT5E: view
genes like me logo Genes that share phenotypes with NT5E: view

Human Phenotype Ontology for NT5E Gene

HPO Id HPO Name Alternative Ids Definition Synonyms

Animal Models for NT5E Gene

MGI Knock Outs for NT5E:

Clone Products

  • Addgene plasmids for NT5E

No data available for Transcription Factor Targets and HOMER Transcription for NT5E Gene

Localization for NT5E Gene

Subcellular locations from UniProtKB/Swiss-Prot for NT5E Gene

Cell membrane; Lipid-anchor, GPI-anchor.

Subcellular locations from

Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for NT5E gene
Compartment Confidence
plasma membrane 5
extracellular 5
cytosol 5
cytoskeleton 2
mitochondrion 2
nucleus 1
lysosome 1

Subcellular locations from the

Human Protein Atlas (HPA)
  • Cytosol (3)
  • Plasma membrane (3)
See all subcellular structures

Gene Ontology (GO) - Cellular Components for NT5E Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005829 cytosol IDA --
GO:0005886 plasma membrane TAS --
GO:0009986 cell surface IDA 19581412
GO:0016020 membrane TAS 2129526
GO:0031225 anchored component of membrane IEA --
genes like me logo Genes that share ontologies with NT5E: view

Pathways & Interactions for NT5E Gene

genes like me logo Genes that share pathways with NT5E: view

Gene Ontology (GO) - Biological Process for NT5E Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006195 purine nucleotide catabolic process TAS --
GO:0006196 AMP catabolic process IEA --
GO:0006259 DNA metabolic process TAS 2129526
GO:0007159 leukocyte cell-cell adhesion IDA 7595232
GO:0009166 nucleotide catabolic process IEA --
genes like me logo Genes that share ontologies with NT5E: view

No data available for SIGNOR curated interactions for NT5E Gene

Drugs & Compounds for NT5E Gene

(32) Drugs for NT5E Gene - From: DrugBank, ClinicalTrials, DGIdb, HMDB, and Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Cytarabine Approved, Investigational Pharma Enzyme, substrate 1136
Pentoxifylline Approved, Investigational Pharma Target, inhibitor 102
Etidronic acid Approved Pharma 83
Adenosine Approved, Investigational Pharma Agonist 528
Phosphoric acid Approved Pharma 0

(25) Additional Compounds for NT5E Gene - From: HMDB, IUPHAR, and Novoseek

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
Inhibition, Inhibitor
beta-nicotinamide D-ribonucleotide
  • 1-(6-Amino-9H-purin-9-yl)-1,2-dideoxy-b-D-Ribofuranose
  • 1-(6-Amino-9H-purin-9-yl)-1,2-dideoxy-beta-D-Ribofuranose
  • 1-(6-Amino-9H-purin-9-yl)-1,2-dideoxy-beta-delta-Ribofuranose
  • 2'-Deoxyadenosine
  • 2-Deoxyadenosine
Deoxyadenosine monophosphate
  • 2'-dAMP
  • 2'-Deoxy-5'-adenylate
  • 2'-Deoxy-5'-adenylic acid
  • 2'-Deoxy-Adenosine 5'-phosphorate
  • 2'-Deoxy-Adenosine 5'-phosphoric acid
  • 2'-Deoxy-Guanosine
  • 2'-Deoxyguanosine
  • 2-Deoxyguanosine
  • 9-(2-Deoxy-b-D-erythro-pentofuranosyl)-Guanine
  • 9-(2-Deoxy-b-D-erythro-pentofuranosyl)guanine
genes like me logo Genes that share compounds with NT5E: view

Transcripts for NT5E Gene

Unigene Clusters for NT5E Gene

5-nucleotidase, ecto (CD73):
Representative Sequences:

Clone Products

  • Addgene plasmids for NT5E

Alternative Splicing Database (ASD) splice patterns (SP) for NT5E Gene

ExUns: 1a · 1b ^ 2 ^ 3a · 3b ^ 4 ^ 5 ^ 6 ^ 7 ^ 8 ^ 9
SP1: -
SP2: - -

Relevant External Links for NT5E Gene

GeneLoc Exon Structure for
ECgene alternative splicing isoforms for

Expression for NT5E Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and CGAP SAGE for NT5E Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Protein differential expression in normal tissues from HIPED for NT5E Gene

This gene is overexpressed in Ovary (10.1) and Bone marrow mesenchymal stem cell (9.4).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MaxQB, and MOPED for NT5E Gene

NURSA nuclear receptor signaling pathways regulating expression of NT5E Gene:


SOURCE GeneReport for Unigene cluster for NT5E Gene:


Evidence on tissue expression from TISSUES for NT5E Gene

  • Blood(4.5)
  • Liver(4.5)
  • Nervous system(4.2)
  • Skin(3.6)
  • Lung(3.5)
  • Bone marrow(3.1)
  • Kidney(2.9)
  • Heart(2.5)
  • Bone(2.4)
  • Muscle(2.3)
  • Eye(2.2)
  • Spleen(2.2)

Phenotype-based relationships between genes and organs from Gene ORGANizer for NT5E Gene

Germ Layers:
  • mesoderm
  • cardiovascular
  • skeleton
  • blood vessel
  • spinal column
  • vertebrae
genes like me logo Genes that share expression patterns with NT5E: view

No data available for mRNA differential expression in normal tissues , Protein tissue co-expression partners and mRNA Expression by UniProt/SwissProt for NT5E Gene

Orthologs for NT5E Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for NT5E Gene

Organism Taxonomy Gene Similarity Type Details
(Pan troglodytes)
Mammalia NT5E 33 34
  • 99.71 (n)
(Canis familiaris)
Mammalia NT5E 33 34
  • 90.64 (n)
(Bos Taurus)
Mammalia -- 34
  • 90 (a)
-- 34
  • 89 (a)
(Rattus norvegicus)
Mammalia Nt5e 33
  • 85.48 (n)
(Mus musculus)
Mammalia Nt5e 33 16 34
  • 85.42 (n)
(Monodelphis domestica)
Mammalia NT5E 34
  • 74 (a)
(Ornithorhynchus anatinus)
Mammalia NT5E 34
  • 73 (a)
(Gallus gallus)
Aves NT5E 33 34
  • 65.75 (n)
(Anolis carolinensis)
Reptilia -- 34
  • 62 (a)
-- 34
  • 55 (a)
tropical clawed frog
(Silurana tropicalis)
Amphibia nt5e 33
  • 65.42 (n)
African clawed frog
(Xenopus laevis)
Amphibia Xl.14455 33
(Danio rerio)
Actinopterygii nt5e 33 34
  • 64.17 (n)
zgc63784 33
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP003629 33
  • 49.97 (n)
fruit fly
(Drosophila melanogaster)
Insecta veil 33 34
  • 48.99 (n)
CG30103 35 34
  • 39 (a)
CG30104 35
  • 39 (a)
CG4827 35
  • 39 (a)
NT5E-2 34
  • 38 (a)
CG1961 35
  • 33 (a)
CG42249 34
  • 29 (a)
CG11883 34
  • 17 (a)
bread mold
(Neurospora crassa)
Ascomycetes NCU09659 33
  • 48.35 (n)
sea squirt
(Ciona savignyi)
Ascidiacea -- 34
  • 13 (a)
Species where no ortholog for NT5E was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for NT5E Gene

Gene Tree for NT5E (if available)
Gene Tree for NT5E (if available)

Paralogs for NT5E Gene

No data available for Paralogs for NT5E Gene

Variants for NT5E Gene

Sequence variations from dbSNP and Humsavar for NT5E Gene

SNP ID Clin Chr 06 pos Sequence Context AA Info Type
rs387906620 Pathogenic, Calcification of joints and arteries (CALJA) [MIM:211800] 85,487,458(+) AGAAT(A/G)CAACA reference, missense
rs373328681 Pathogenic 85,471,336(+) ACATT(A/C/T)GGGTT reference, missense, stop-gained
rs1000056641 -- 85,450,378(+) GCTGC(G/T)GCTGG reference, missense
rs1000059895 -- 85,457,009(+) TGCCG(G/T)CTGTA intron-variant
rs1000169347 -- 85,450,697(+) TGGGA(A/G)TATTT intron-variant

Structural Variations from Database of Genomic Variants (DGV) for NT5E Gene

Variant ID Type Subtype PubMed ID
nsv604093 CNV loss 21841781
nsv604092 CNV loss 21841781
nsv520112 CNV loss 19592680
nsv470839 CNV loss 18288195
nsv463939 CNV loss 19166990

Variation tolerance for NT5E Gene

Residual Variation Intolerance Score: 76.1% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 4.63; 65.53% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for NT5E Gene

Human Gene Mutation Database (HGMD)
SNPedia medical, phenotypic, and genealogical associations of SNPs for

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for NT5E Gene

Disorders for NT5E Gene

MalaCards: The human disease database

(4) MalaCards diseases for NT5E Gene - From: HGMD, OMIM, ClinVar, Orphanet, Swiss-Prot, DISEASES, and GeneCards

Disorder Aliases PubMed IDs
calcification of joints and arteries
  • hereditary arterial and articular multiple calcification syndrome
intestinal tuberculosis
  • tuberculosis of intestine
  • idiopathic calciphylaxis
arterial calcification of infancy
  • generalized arterial calcification of infancy
- elite association - COSMIC cancer census association via MalaCards
Search NT5E in MalaCards View complete list of genes associated with diseases


  • Calcification of joints and arteries (CALJA) [MIM:211800]: A condition characterized by adult-onset calcification of the lower extremity arteries, including the iliac, femoral and tibial arteries, and hand and foot capsule joints. Age of onset has been reported as early as the second decade of life, usually involving intense joint pain or calcification in the hands. {ECO:0000269 PubMed:21288095, ECO:0000269 PubMed:24887587}. Note=The disease is caused by mutations affecting the gene represented in this entry.

Relevant External Links for NT5E

Genetic Association Database (GAD)
Human Genome Epidemiology (HuGE) Navigator
Atlas of Genetics and Cytogenetics in Oncology and Haematology:
genes like me logo Genes that share disorders with NT5E: view

No data available for Genatlas for NT5E Gene

Publications for NT5E Gene

  1. Isolation and characterization of the promoter of the human 5'-nucleotidase (CD73)-encoding gene. (PMID: 8566797) Hansen KR … Thompson LF (Gene 1995) 3 4 22 60
  2. NT5E mutations that cause human disease are associated with intracellular mistrafficking of NT5E protein. (PMID: 24887587) Fausther M … Dranoff JA (PloS one 2014) 3 4 60
  3. The high-resolution crystal structure of periplasmic Haemophilus influenzae NAD nucleotidase reveals a novel enzymatic function of human CD73 related to NAD metabolism. (PMID: 21933152) Garavaglia S … Rizzi M (The Biochemical journal 2012) 3 4 60
  4. Crystal structure of the human ecto-5'-nucleotidase (CD73): insights into the regulation of purinergic signaling. (PMID: 23142347) Knapp K … Sträter N (Structure (London, England : 1993) 2012) 3 4 60
  5. NT5E mutations and arterial calcifications. (PMID: 21288095) St Hilaire C … Boehm M (The New England journal of medicine 2011) 3 4 60

Products for NT5E Gene

  • Addgene plasmids for NT5E

Sources for NT5E Gene