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Aliases for NT5E Gene

Aliases for NT5E Gene

  • 5'-Nucleotidase, Ecto (CD73) 2
  • NT5 3 4 6
  • 5-Nucleotidase, Ecto (CD73) 2 3
  • Ecto-5-Nucleotidase 3 4
  • EC 4 63
  • 5-NT 3 4
  • NTE 3 4
  • Purine 5-Prime-Nucleotidase 3
  • 5 Nucleotidase (CD73) 2
  • 5-Nucleotidase 3
  • CD73 Antigen 4
  • CALJA 3
  • CD73 3
  • E5NT 3
  • ENT 3
  • NT 3
  • EN 3

External Ids for NT5E Gene

Previous HGNC Symbols for NT5E Gene

  • NT5

Previous GeneCards Identifiers for NT5E Gene

  • GC06P086106
  • GC06P086155
  • GC06P086216
  • GC06P083383

Summaries for NT5E Gene

Entrez Gene Summary for NT5E Gene

  • The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011]

GeneCards Summary for NT5E Gene

NT5E (5'-Nucleotidase, Ecto (CD73)) is a Protein Coding gene. Diseases associated with NT5E include calcification of joints and arteries and hereditary arterial and articular multiple calcification syndrome. Among its related pathways are Metabolism and Metabolism. GO annotations related to this gene include nucleotide binding.

UniProtKB/Swiss-Prot for NT5E Gene

  • Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Exhibits AMP-, NAD-, and NMN-nucleosidase activities.

Gene Wiki entry for NT5E Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for NT5E Gene

Genomics for NT5E Gene

Regulatory Elements for NT5E Gene

Epigenetics Products

  • DNA Methylation CpG Assay Predesigned for Pyrosequencing in human,mouse,rat

Genomic Location for NT5E Gene

85,449,584 bp from pter
85,495,791 bp from pter
46,208 bases
Plus strand

Genomic View for NT5E Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for NT5E Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for NT5E Gene

Proteins for NT5E Gene

  • Protein details for NT5E Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Recommended name:
    Protein Accession:
    Secondary Accessions:
    • B3KQI8
    • O75520
    • Q5W116

    Protein attributes for NT5E Gene

    574 amino acids
    Molecular mass:
    63368 Da
    Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
    Quaternary structure:
    • Homodimer.

    Three dimensional structures from OCA and Proteopedia for NT5E Gene

    Alternative splice isoforms for NT5E Gene


neXtProt entry for NT5E Gene

Proteomics data for NT5E Gene at MOPED

Post-translational modifications for NT5E Gene

  • Glycosylation at Asn53, Asn311, Asn333, and Asn403
  • Modification sites at PhosphoSitePlus

Other Protein References for NT5E Gene

Antibody Products

  • R&D Systems Antibodies for NT5E (5'-Nucleotidase/CD73)
  • Cell Signaling Technology (CST) Antibodies for NT5E (NT5E)

Domains for NT5E Gene

Gene Families for NT5E Gene

Graphical View of Domain Structure for InterPro Entry



  • Belongs to the 5-nucleotidase family.
  • Belongs to the 5-nucleotidase family.
genes like me logo Genes that share domains with NT5E: view

Function for NT5E Gene

Molecular function for NT5E Gene

UniProtKB/Swiss-Prot CatalyticActivity:
A 5-ribonucleotide + H(2)O = a ribonucleoside + phosphate
UniProtKB/Swiss-Prot Function:
Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Exhibits AMP-, NAD-, and NMN-nucleosidase activities.

Enzyme Numbers (IUBMB) for NT5E Gene

Gene Ontology (GO) - Molecular Function for NT5E Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000166 nucleotide binding IEA --
GO:0008253 5-nucleotidase activity IDA 7595232
GO:0016787 hydrolase activity --
GO:0016788 hydrolase activity, acting on ester bonds --
GO:0046872 metal ion binding IEA --
genes like me logo Genes that share ontologies with NT5E: view
genes like me logo Genes that share phenotypes with NT5E: view

Animal Models for NT5E Gene

MGI Knock Outs for NT5E:

Animal Model Products

No data available for Transcription Factor Targets and HOMER Transcription for NT5E Gene

Localization for NT5E Gene

Subcellular locations from UniProtKB/Swiss-Prot for NT5E Gene

Cell membrane; Lipid-anchor, GPI-anchor.

Subcellular locations from

Jensen Localization Image for NT5E Gene COMPARTMENTS Subcellular localization image for NT5E gene
Compartment Confidence
extracellular 5
plasma membrane 5
cytosol 3
endoplasmic reticulum 3
mitochondrion 3
golgi apparatus 2
lysosome 2
nucleus 2
vacuole 2
cytoskeleton 1
endosome 1

Gene Ontology (GO) - Cellular Components for NT5E Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005737 cytoplasm IDA --
GO:0005886 plasma membrane TAS --
GO:0009986 cell surface IDA 19581412
GO:0016020 membrane TAS 2129526
GO:0031225 anchored component of membrane IEA --
genes like me logo Genes that share ontologies with NT5E: view

Pathways for NT5E Gene

genes like me logo Genes that share pathways with NT5E: view

PCR Array Products

  • Pathway & Disease-focused RT² Profiler PCR Arrays

Gene Ontology (GO) - Biological Process for NT5E Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006144 purine nucleobase metabolic process TAS --
GO:0006195 purine nucleotide catabolic process TAS --
GO:0006196 AMP catabolic process IEA --
GO:0006206 pyrimidine nucleobase metabolic process TAS --
GO:0006259 DNA metabolic process TAS 2129526
genes like me logo Genes that share ontologies with NT5E: view

Drugs for NT5E Gene

(2) Drugbank Compounds for NT5E Gene

Compound Status Synonyms Cas Number PubChem ID Type Actions PubMed IDs
  • 1-beta-D-Arabinofuranosylcytosine
147-94-4 6253 Enzyme substrate approved, investigational
  • Oxpentifylline
6493-05-6 4740 Target inhibitor approved, investigational

(28) HMDB Compounds for NT5E Gene

Compound Synonyms Cas Number PubMed IDs
2'-Deoxyguanosine 5'-monophosphate
  • 2'-Deoxy-5'-GMP
5-Thymidylic acid
  • 2'-Deoxythymidine 5'-monophosphate
  • 1-(6-Amino-9H-purin-9-yl)-1-deoxy-beta-D-Ribofuranose
Adenosine monophosphate
  • 5'-Adenosine monophosphate
beta-nicotinamide D-ribonucleotide

(1) IUPHAR Ligand for NT5E Gene

Ligand Type Action Affinity Pubmed IDs
alphabeta-methyleneADP Inhibitor Inhibition 8.7

(24) Novoseek inferred chemical compound relationships for NT5E Gene

Compound -log(P) Hits PubMed IDs
apcp 87.9 4
epsilon-amp 86.8 4
alpha,beta-methyleneadenosine-5-diphosphate 83.6 1
purine 57 11
cyclic adp-ribose 42.9 4
genes like me logo Genes that share compounds with NT5E: view

Transcripts for NT5E Gene

Unigene Clusters for NT5E Gene

5-nucleotidase, ecto (CD73):
Representative Sequences:

Alternative Splicing Database (ASD) splice patterns (SP) for NT5E Gene

ExUns: 1a · 1b ^ 2 ^ 3a · 3b ^ 4 ^ 5 ^ 6 ^ 7 ^ 8 ^ 9
SP1: -
SP2: - -

Relevant External Links for NT5E Gene

GeneLoc Exon Structure for
ECgene alternative splicing isoforms for

Expression for NT5E Gene

mRNA expression in normal human tissues for NT5E Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Protein differential expression in normal tissues for NT5E Gene

This gene is overexpressed in Ovary (10.1) and Bone marrow mesenchymal stem cell (9.4).

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, MOPED, and MaxQB for NT5E Gene

SOURCE GeneReport for Unigene cluster for NT5E Gene Hs.153952

genes like me logo Genes that share expressions with NT5E: view

Primer Products

In Situ Assay Products

No data available for mRNA differential expression in normal tissues , mRNA Expression by UniProt/SwissProt and Expression partners for NT5E Gene

Orthologs for NT5E Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for NT5E Gene

Organism Taxonomy Gene Similarity Type Details
(Canis familiaris)
Mammalia NT5E 35
  • 90.64 (n)
  • 90.71 (a)
NT5E 36
  • 90 (a)
(Mus musculus)
Mammalia Nt5e 35
  • 85.42 (n)
  • 86.76 (a)
Nt5e 16
Nt5e 36
  • 86 (a)
(Pan troglodytes)
Mammalia NT5E 35
  • 99.71 (n)
  • 99.83 (a)
NT5E 36
  • 100 (a)
(Rattus norvegicus)
Mammalia Nt5e 35
  • 85.48 (n)
  • 87.8 (a)
(Bos Taurus)
Mammalia -- 36
  • 89 (a)
-- 36
  • 90 (a)
(Monodelphis domestica)
Mammalia NT5E 36
  • 74 (a)
(Ornithorhynchus anatinus)
Mammalia NT5E 36
  • 73 (a)
(Gallus gallus)
Aves NT5E 35
  • 65.75 (n)
  • 65.01 (a)
NT5E 36
  • 62 (a)
(Anolis carolinensis)
Reptilia -- 36
  • 62 (a)
-- 36
  • 55 (a)
tropical clawed frog
(Silurana tropicalis)
Amphibia nt5e 35
  • 65.42 (n)
  • 67.2 (a)
African clawed frog
(Xenopus laevis)
Amphibia Xl.14455 35
(Danio rerio)
Actinopterygii nt5e 35
  • 64.17 (n)
  • 65 (a)
zgc63784 35
nt5e 36
  • 63 (a)
fruit fly
(Drosophila melanogaster)
Insecta CG1961 37
  • 33 (a)
CG30103 37
  • 39 (a)
CG30104 37
  • 39 (a)
CG4827 37
  • 39 (a)
veil 35
  • 48.99 (n)
  • 41.44 (a)
CG11883 36
  • 17 (a)
CG30103 36
  • 36 (a)
CG42249 36
  • 29 (a)
NT5E-2 36
  • 38 (a)
veil 36
  • 37 (a)
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP003629 35
  • 49.97 (n)
  • 44.26 (a)
bread mold
(Neurospora crassa)
Ascomycetes NCU09659 35
  • 48.35 (n)
  • 34.95 (a)
sea squirt
(Ciona savignyi)
Ascidiacea -- 36
  • 13 (a)
Species with no ortholog for NT5E:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for NT5E Gene

Gene Tree for NT5E (if available)
Gene Tree for NT5E (if available)

Paralogs for NT5E Gene

No data available for Paralogs for NT5E Gene

Variants for NT5E Gene

Sequence variations from dbSNP and Humsavar for NT5E Gene

SNP ID Clin Chr 06 pos Sequence Context AA Info Type MAF
rs144709340 -- 85,489,837(+) TGCAG(G/T)TAAGG intron-variant
rs144719925 -- 85,489,590(+) ATGAA(C/T)GCAAC reference, missense
rs144851417 -- 85,482,001(+) AAAAG(A/G)AGCAT intron-variant
rs144579821 -- 85,456,822(+) CTGGG(A/T)TCAAG intron-variant
rs144566178 -- 85,470,458(+) ATTTA(A/T)ATATT intron-variant

Structural Variations from Database of Genomic Variants (DGV) for NT5E Gene

Variant ID Type Subtype PubMed ID
nsv520112 CNV Loss 19592680
nsv886333 CNV Loss 21882294
nsv463939 CNV Loss 19166990
nsv470839 CNV Loss 18288195

Relevant External Links for NT5E Gene

HapMap Linkage Disequilibrium report
Human Gene Mutation Database (HGMD)

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for NT5E Gene

Disorders for NT5E Gene

(1) OMIM Diseases for NT5E Gene (129190)


  • Calcification of joints and arteries (CALJA) [MIM:211800]: A condition characterized by adult-onset calcification of the lower extremity arteries, including the iliac, femoral and tibial arteries, and hand and foot capsule joints. Age of onset has been reported as early as the second decade of life, usually involving intense joint pain or calcification in the hands. {ECO:0000269 PubMed:21288095}. Note=The disease is caused by mutations affecting the gene represented in this entry.

(4) University of Copenhagen DISEASES for NT5E Gene

(12) Novoseek inferred disease relationships for NT5E Gene

Disease -log(P) Hits PubMed IDs
leukemia t-cell 30.5 2
zinc deficiency 20.4 3
inflammation 15.8 3
leukemia 0 5
primary tumor 0 2

Relevant External Links for NT5E

Genetic Association Database (GAD)
Human Genome Epidemiology (HuGE) Navigator
genes like me logo Genes that share disorders with NT5E: view

No data available for Genatlas for NT5E Gene

Publications for NT5E Gene

  1. Isolation and characterization of the promoter of the human 5'- nucleotidase (CD73)-encoding gene. (PMID: 8566797) Hansen K.R. … Thompson L.F. (Gene 1995) 3 4 23
  2. Production and characterization of monoclonal antibodies to the glycosyl phosphatidylinositol-anchored lymphocyte differentiation antigen ecto-5'-nucleotidase (CD73). (PMID: 2137649) Thomson L.F. … Lucas A.H. (Tissue Antigens 1990) 3 23
  3. CD73 is involved in lymphocyte binding to the endothelium: characterization of lymphocyte-vascular adhesion protein 2 identifies it as CD73. (PMID: 7595232) Airas L. … Jalkanen S. (J. Exp. Med. 1995) 3 23
  4. Expression of ecto-5'-nucleotidase (eN, CD73) in cell lines from various stages of human melanoma. (PMID: 16718268) Sadej R. … Skladanowski A.C. (Melanoma Res. 2006) 3 23
  5. CD73 engagement promotes lymphocyte binding to endothelial cells via a lymphocyte function-associated antigen-1-dependent mechanism. (PMID: 11067892) Airas L. … Jalkanen S. (J. Immunol. 2000) 3 23

Products for NT5E Gene

Sources for NT5E Gene

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