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Aliases for NT5E Gene

Aliases for NT5E Gene

  • 5'-Nucleotidase Ecto 2
  • 5-Nucleotidase, Ecto (CD73) 2 3 5
  • Ecto-5-Nucleotidase 3 4
  • 5-Nucleotidase Ecto 2 3
  • EC 4 63
  • 5-NT 3 4
  • NT5 3 4
  • NTE 3 4
  • Purine 5-Prime-Nucleotidase 3
  • 5 Nucleotidase (CD73) 2
  • CD73 Antigen 4
  • CALJA 3
  • CD73 3
  • E5NT 3
  • ENT 3
  • NT 3
  • EN 3

External Ids for NT5E Gene

Previous HGNC Symbols for NT5E Gene

  • NT5

Previous GeneCards Identifiers for NT5E Gene

  • GC06P086106
  • GC06P086155
  • GC06P086216
  • GC06P083383

Summaries for NT5E Gene

Entrez Gene Summary for NT5E Gene

  • The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2011]

GeneCards Summary for NT5E Gene

NT5E (5'-Nucleotidase Ecto) is a Protein Coding gene. Diseases associated with NT5E include Calcification Of Joints And Arteries and Glaucoma 1, Open Angle, 1O. Among its related pathways are Purine metabolism and NF-kappaB Signaling. GO annotations related to this gene include nucleotide binding and hydrolase activity, acting on ester bonds.

UniProtKB/Swiss-Prot for NT5E Gene

  • Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Exhibits AMP-, NAD-, and NMN-nucleosidase activities.

Gene Wiki entry for NT5E Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for NT5E Gene

Genomics for NT5E Gene

Regulatory Elements for NT5E Gene

Enhancers for NT5E Gene
GeneHancer Identifier Score Enhancer Sources TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor Binding Sites within enhancer Other Gene Targets for Enhancer

Enhancers around NT5E on UCSC Golden Path with GeneCards custom track

Promoters for NT5E Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters

ENSRs around NT5E on UCSC Golden Path with GeneCards custom track

Genomic Location for NT5E Gene

85,449,584 bp from pter
85,495,791 bp from pter
46,208 bases
Plus strand

Genomic View for NT5E Gene

Genes around NT5E on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
NT5E Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for NT5E Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for NT5E Gene

Proteins for NT5E Gene

  • Protein details for NT5E Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Recommended name:
    Protein Accession:
    Secondary Accessions:
    • B3KQI8
    • O75520
    • Q5W116

    Protein attributes for NT5E Gene

    574 amino acids
    Molecular mass:
    63368 Da
    Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
    Quaternary structure:
    • Homodimer.

    Three dimensional structures from OCA and Proteopedia for NT5E Gene

    Alternative splice isoforms for NT5E Gene


neXtProt entry for NT5E Gene

Proteomics data for NT5E Gene at MOPED

Post-translational modifications for NT5E Gene

  • Glycosylation at Asn 53, Asn 311, Asn 333, and Asn 403
  • Modification sites at PhosphoSitePlus

Other Protein References for NT5E Gene

Antibody Products

  • R&D Systems Antibodies for NT5E (5'-Nucleotidase/CD73)
  • Cell Signaling Technology (CST) Antibodies for NT5E (NT5E)

Domains & Families for NT5E Gene

Gene Families for NT5E Gene

Graphical View of Domain Structure for InterPro Entry



  • Belongs to the 5-nucleotidase family.
  • Belongs to the 5-nucleotidase family.
genes like me logo Genes that share domains with NT5E: view

Function for NT5E Gene

Molecular function for NT5E Gene

UniProtKB/Swiss-Prot CatalyticActivity:
A 5-ribonucleotide + H(2)O = a ribonucleoside + phosphate.
UniProtKB/Swiss-Prot Function:
Hydrolyzes extracellular nucleotides into membrane permeable nucleosides. Exhibits AMP-, NAD-, and NMN-nucleosidase activities.

Enzyme Numbers (IUBMB) for NT5E Gene

Gene Ontology (GO) - Molecular Function for NT5E Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0008253 5-nucleotidase activity IDA 7595232
genes like me logo Genes that share ontologies with NT5E: view
genes like me logo Genes that share phenotypes with NT5E: view

Human Phenotype Ontology for NT5E Gene

HPO Id HPO Name Alternative Ids Definition Synonyms

Animal Models for NT5E Gene

MGI Knock Outs for NT5E:

Animal Model Products

No data available for Transcription Factor Targets and HOMER Transcription for NT5E Gene

Localization for NT5E Gene

Subcellular locations from UniProtKB/Swiss-Prot for NT5E Gene

Cell membrane; Lipid-anchor, GPI-anchor.

Subcellular locations from

Jensen Localization Image for NT5E Gene COMPARTMENTS Subcellular localization image for NT5E gene
Compartment Confidence
extracellular 5
plasma membrane 5
cytosol 3
cytoskeleton 2
mitochondrion 2
lysosome 1
nucleus 1
vacuole 1

Gene Ontology (GO) - Cellular Components for NT5E Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005737 cytoplasm IDA --
genes like me logo Genes that share ontologies with NT5E: view

Pathways & Interactions for NT5E Gene

genes like me logo Genes that share pathways with NT5E: view

Gene Ontology (GO) - Biological Process for NT5E Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006164 purine nucleotide biosynthetic process IEA --
GO:0009166 nucleotide catabolic process IEA --
GO:0010044 response to aluminum ion IEA --
GO:0044281 small molecule metabolic process TAS --
GO:0046085 adenosine metabolic process IEA --
genes like me logo Genes that share ontologies with NT5E: view

No data available for SIGNOR curated interactions for NT5E Gene

Drugs & Compounds for NT5E Gene

(29) Drugs for NT5E Gene - From: DrugBank, ClinicalTrials, DGIdb, HMDB, and Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Cytarabine Approved, Investigational Pharma Enzyme, substrate 1054
Pentoxifylline Approved, Investigational Pharma Target, inhibitor 92
Etidronic acid Approved Pharma 83
Adenosine Approved, Investigational Pharma Agonist 384
Adenosine monophosphate Approved Nutra 0

(28) Additional Compounds for NT5E Gene - From: Novoseek, HMDB, and IUPHAR

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
Inhibition, Inhibitor
beta-nicotinamide D-ribonucleotide
Cytidine monophosphate
  • 5'-CMP
  • 5-Cytidylate
  • 5-Cytidylic acid
  • CMP
  • Cytidine 5'-monophosphate
  • 1-(6-Amino-9H-purin-9-yl)-1,2-dideoxy-b-D-Ribofuranose
  • 1-(6-Amino-9H-purin-9-yl)-1,2-dideoxy-beta-D-Ribofuranose
  • 1-(6-Amino-9H-purin-9-yl)-1,2-dideoxy-beta-delta-Ribofuranose
  • 2'-Deoxyadenosine
  • 2-Deoxyadenosine
Deoxyadenosine monophosphate
  • 2'-dAMP
  • 2'-Deoxy-5'-adenylate
  • 2'-Deoxy-5'-adenylic acid
  • 2'-Deoxy-Adenosine 5'-phosphorate
  • 2'-Deoxy-Adenosine 5'-phosphoric acid
genes like me logo Genes that share compounds with NT5E: view

Transcripts for NT5E Gene

Unigene Clusters for NT5E Gene

5-nucleotidase, ecto (CD73):
Representative Sequences:

Alternative Splicing Database (ASD) splice patterns (SP) for NT5E Gene

ExUns: 1a · 1b ^ 2 ^ 3a · 3b ^ 4 ^ 5 ^ 6 ^ 7 ^ 8 ^ 9
SP1: -
SP2: - -

Relevant External Links for NT5E Gene

GeneLoc Exon Structure for
ECgene alternative splicing isoforms for

Expression for NT5E Gene

mRNA expression in normal human tissues for NT5E Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Protein differential expression in normal tissues from HIPED for NT5E Gene

This gene is overexpressed in Ovary (10.1) and Bone marrow mesenchymal stem cell (9.4).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MOPED, and MaxQB for NT5E Gene

SOURCE GeneReport for Unigene cluster for NT5E Gene Hs.153952

genes like me logo Genes that share expression patterns with NT5E: view

Primer Products

No data available for mRNA differential expression in normal tissues , mRNA Expression by UniProt/SwissProt and Protein tissue co-expression partners for NT5E Gene

Orthologs for NT5E Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for NT5E Gene

Organism Taxonomy Gene Similarity Type Details
(Canis familiaris)
Mammalia NT5E 35
  • 90.64 (n)
  • 90.71 (a)
NT5E 36
  • 90 (a)
(Mus musculus)
Mammalia Nt5e 35
  • 85.42 (n)
  • 86.76 (a)
Nt5e 16
Nt5e 36
  • 86 (a)
(Pan troglodytes)
Mammalia NT5E 35
  • 99.71 (n)
  • 99.83 (a)
NT5E 36
  • 100 (a)
(Rattus norvegicus)
Mammalia Nt5e 35
  • 85.48 (n)
  • 87.8 (a)
(Bos Taurus)
Mammalia -- 36
  • 89 (a)
-- 36
  • 90 (a)
(Monodelphis domestica)
Mammalia NT5E 36
  • 74 (a)
(Ornithorhynchus anatinus)
Mammalia NT5E 36
  • 73 (a)
(Gallus gallus)
Aves NT5E 35
  • 65.75 (n)
  • 65.01 (a)
NT5E 36
  • 62 (a)
(Anolis carolinensis)
Reptilia -- 36
  • 62 (a)
-- 36
  • 55 (a)
tropical clawed frog
(Silurana tropicalis)
Amphibia nt5e 35
  • 65.42 (n)
  • 67.2 (a)
African clawed frog
(Xenopus laevis)
Amphibia Xl.14455 35
(Danio rerio)
Actinopterygii nt5e 35
  • 64.17 (n)
  • 65 (a)
zgc63784 35
nt5e 36
  • 63 (a)
fruit fly
(Drosophila melanogaster)
Insecta CG1961 37
  • 33 (a)
CG30103 37
  • 39 (a)
CG30104 37
  • 39 (a)
CG4827 37
  • 39 (a)
veil 35
  • 48.99 (n)
  • 41.44 (a)
CG11883 36
  • 17 (a)
CG30103 36
  • 36 (a)
CG42249 36
  • 29 (a)
NT5E-2 36
  • 38 (a)
veil 36
  • 37 (a)
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP003629 35
  • 49.97 (n)
  • 44.26 (a)
bread mold
(Neurospora crassa)
Ascomycetes NCU09659 35
  • 48.35 (n)
  • 34.95 (a)
sea squirt
(Ciona savignyi)
Ascidiacea -- 36
  • 13 (a)
Species with no ortholog for NT5E:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for NT5E Gene

Gene Tree for NT5E (if available)
Gene Tree for NT5E (if available)

Paralogs for NT5E Gene

No data available for Paralogs for NT5E Gene

Variants for NT5E Gene

Sequence variations from dbSNP and Humsavar for NT5E Gene

SNP ID Clin Chr 06 pos Sequence Context AA Info Type
rs2229523 - 85,489,515(+) GACAC(A/G)CGGAT reference, missense
rs2229524 - 85,489,525(+) TGAAA(A/C/G/T)GTTCT reference, missense
VAR_065185 Calcification of joints and arteries (CALJA)
rs5877938 -- 85,477,038(+) ACCTA(-/G)TATTT intron-variant
rs6454469 -- 85,476,757(+) tcccc(A/G)gctga intron-variant

Structural Variations from Database of Genomic Variants (DGV) for NT5E Gene

Variant ID Type Subtype PubMed ID
nsv520112 CNV Loss 19592680
nsv886333 CNV Loss 21882294
nsv463939 CNV Loss 19166990
nsv470839 CNV Loss 18288195

Variation tolerance for NT5E Gene

Residual Variation Intolerance Score: 76.1% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 4.63; 65.53% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for NT5E Gene

Human Gene Mutation Database (HGMD)

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for NT5E Gene

Disorders for NT5E Gene

MalaCards: The human disease database

(6) MalaCards diseases for NT5E Gene - From: OMIM, ClinVar, Orphanet, Swiss-Prot, DISEASES, and GeneCards

Disorder Aliases PubMed IDs
calcification of joints and arteries
  • calja
glaucoma 1, open angle, 1o
  • glaucoma 1, open angle, o
hereditary arterial and articular multiple calcification syndrome
  • calcification of joints and arteries
intestinal tuberculosis
  • tuberculosis of intestine
nut allergy
  • allergy to nuts
- elite association - COSMIC cancer census association via MalaCards
Search NT5E in MalaCards View complete list of genes associated with diseases


  • Calcification of joints and arteries (CALJA) [MIM:211800]: A condition characterized by adult-onset calcification of the lower extremity arteries, including the iliac, femoral and tibial arteries, and hand and foot capsule joints. Age of onset has been reported as early as the second decade of life, usually involving intense joint pain or calcification in the hands. {ECO:0000269 PubMed:21288095}. Note=The disease is caused by mutations affecting the gene represented in this entry.

Relevant External Links for NT5E

Genetic Association Database (GAD)
Human Genome Epidemiology (HuGE) Navigator
Atlas of Genetics and Cytogenetics in Oncology and Haematology:
genes like me logo Genes that share disorders with NT5E: view

No data available for Genatlas for NT5E Gene

Publications for NT5E Gene

  1. Isolation and characterization of the promoter of the human 5'- nucleotidase (CD73)-encoding gene. (PMID: 8566797) Hansen K.R. … Thompson L.F. (Gene 1995) 3 4 23 67
  2. [Role of PKC in regulation of CD73 by lysophosphatidylcholine in human endothelial cells]. (PMID: 20476579) Zhang Q.Y. … Tan J. (Zhongguo Ying Yong Sheng Li Xue Za Zhi 2010) 3 23
  3. Ecto-5'-nucleotidase promotes invasion, migration and adhesion of human breast cancer cells. (PMID: 17671792) Wang L. … Zhou P. (J. Cancer Res. Clin. Oncol. 2008) 3 23
  4. CD73 participates in cellular multiresistance program and protects against TRAIL-induced apoptosis. (PMID: 18566412) Mikhailov A. … Eriksson J.E. (J. Immunol. 2008) 3 23
  5. PMNs facilitate translocation of platelets across human and mouse epithelium and together alter fluid homeostasis via epithelial cell-expressed ecto-NTPDases. (PMID: 18924612) WeissmA1ller T. … Colgan S.P. (J. Clin. Invest. 2008) 3 23

Products for NT5E Gene

Sources for NT5E Gene