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Category Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21

GIFtS
-

NT5E Gene

protein-coding GIFtS: 68
GCID: GC06P086216

5'-nucleotidase, ecto (CD73)

(Previous name: 5' nucleotidase (CD73) )
(Previous symbol: NT5)

Explore 44 diseases affiliated with
NT5E via

MalaCards

our new
Human Malady Compendium

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc, ⁷Ensembl, ⁸DME, ⁹miRBase, and/or ¹⁰fRNAdb)
About This Section

Aliases
5'-Nucleotidase, Ecto (CD73)¹ ²		5' Nucleotidase (CD73)¹
NT5¹ ² ³ ⁵		5'-Nucleotidase¹
CD73¹ ²		E5NT²
EN¹		NT²
ENT¹		Ecto-5'-Nucleotidase¹
5'-NT² ³		Purine 5-Prime-Nucleotidase²
NTE² ³		Ecto-5'-Nucleotidase¹
EC 3.1.3.5³ ⁸		CD73 Antigen³

External Ids:	HGNC: 8021¹	Entrez Gene: 4907²	Ensembl: ENSG00000135318⁷	OMIM: 129190⁵	UniProtKB: P21589³

Export aliases for NT5E gene to outside databases

Previous GC identifers: GC06P086106 GC06P086155 GC06P083383

(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for NT5E:

The protein encoded by this gene is a plasma membrane protein that catalyzes the conversion of extracellular

nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte

differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants

encoding different isoforms have been found for this gene.(provided by RefSeq, Mar 2011)

UniProtKB/Swiss-Prot: 5NTD_HUMAN, P21589

Function: Hydrolyzes extracellular nucleotides into membrane permeable nucleosides

Gene Wiki entry for NT5E

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section

RefSeq DNA sequence:

NC_000006.11 NC_018917.1 NT_007299.13

Regulatory elements:

SABiosciences Regulatory transcription factor binding sites in the NT5E gene promoter:
GR AP-1 GR-beta ATF-2 SREBP-1c SREBP-1b SREBP-1a c-Jun GR-alpha
Other transcription factors

SwitchGear Promoter luciferase reporter plasmid: NT5E promoter sequence

Search SABiosciences Chromatin IP Primers for NT5E

Epigenetics:

QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat NT5E

Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 6q14-q21 Ensembl cytogenetic band: 6q14.3 HGNC cytogenetic band: 6q14-q21

NT5E Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
NT5E gene location

GeneLoc information about chromosome 6 GeneLoc Exon Structure

GeneLoc location for GC06P086216: view genomic region (about GC identifiers)

Start:	86,159,302 bp from pter	End:	86,205,500 bp from pter
Size:	46,199 bases	Orientation:	plus strand

(According to ¹UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to ²PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: 5NTD_HUMAN, P21589 (See protein sequence)

Recommended Name: 5'-nucleotidase precursor

Size: 574 amino acids; 63368 Da

Cofactor: Zinc

Subunit: Homodimer; disulfide-linked

Subcellular location: Cell membrane; Lipid-anchor, GPI-anchor

6 PDB 3D structures from and Proteopedia for NT5E:

4H1S (3D)

4H1Y (3D)

4H2B (3D)

4H2F (3D)

4H2G (3D)

4H2I (3D)

Secondary accessions: B3KQI8 O75520 Q5W116

Alternative splicing: 2 isoforms: P21589-1 P21589-2 (No experimental confirmation available)

Explore the universe of human proteins at neXtProt for NT5E: NX_P21589

Post-translational modifications:

View modification sites using PhosphoSitePlus²

View neXtProt modification sites for NX_P21589

4/10 DME Specific Peptides for NT5E (P21589) (see all 10)

YISKMKV

TGEFLQV

GNPILLNS

YPAVEGRI

NT5E Protein expression data from MOPED and PaxDb: About this image

REFSEQ proteins (2 alternative transcripts):

NP_001191742.1 NP_002517.1

ENSEMBL proteins:

ENSP00000257770 ENSP00000358660 ENSP00000414674 ENSP00000387630 ENSP00000358665

Reactome Protein details: P21589
Human Recombinant Protein Products:

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	Novus Biologicals NT5E Protein Novus Biologicals NT5E Lysate
	Sino Biological Recombinant Protein for NT5E
	Browse ProSpec Recombinant Proteins
	Uscn Proteins for NT5E

Gene Ontology (GO): 5/6 cellular component terms (GO ID links to tree view) (see all 6): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005624	membrane fraction	--	--
GO:0005737	cytoplasm	IDA	--
GO:0005886	plasma membrane	TAS	--
GO:0016020	membrane	TAS	2129526
GO:0031225	anchored to membrane	IEA	--

NT5E for ontologies About GeneDecksing

NT5E Antibody Products:

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	Novus Biologicals NT5E Antibodies
	Abcam antibodies for NT5E
	Uscn Antibodies for NT5E
	ThermoFisher Antibodies for NT5E

Assay Products for NT5E:

	Browse Kits and Assays available from EMD Millipore
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	GenScript Custom Assay Services for NT5E
	Browse Enzo Life Sciences for kits & assays
	Uscn ELISAs and CLIAs for NT5E

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

NT5E for domains About GeneDecksing

4 InterPro domains/families:

IPR004843 Metallo_PEstase_dom

IPR006146 5'-Nucleotdase_CS

IPR008334 5'-Nucleotdase_C

IPR006179 5_nucleotidase/apyrase

Graphical View of Domain Structure for InterPro Entry P21589

ProtoNet protein and cluster: P21589

2 Blocks protein families:

IPB006179 Apyrase family signature

IPB008334 -Nucleotdase_C

UniProtKB/Swiss-Prot: 5NTD_HUMAN, P21589

Similarity: Belongs to the 5'-nucleotidase family

(According to ¹UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or ²DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
About This Section

Function Summary:

UniProtKB/Swiss-Prot: 5NTD_HUMAN, P21589

Function: Hydrolyzes extracellular nucleotides into membrane permeable nucleosides

Catalytic activity: A 5'-ribonucleotide + H(2)O = a ribonucleoside + phosphate

Enzyme Number (IUBMB): EC 3.1.3.5¹ ²

miRNA Products:		miRTarBase miRNAs that target NT5E: hsa-mir-155 (MIRT001529), hsa-mir-30a (MIRT005130)

		OriGene 3'-UTR Clone: NT5E Browse MicroRNA Expression Plasmids
		QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat NT5E
		8/19 QIAGEN miScript miRNA Assays for microRNAs that regulate NT5E (see all 19):
		hsa-miR-411* hsa-miR-30c hsa-miR-193a-3p hsa-miR-379* hsa-miR-657 hsa-miR-578 hsa-miR-30d hsa-miR-4260

		SwitchGear 3'UTR luciferase reporter plasmid: NT5E 3' UTR sequence

Inhib. RNA Products:		Browse for Gene Knock-down Tools from EMD Millipore
		OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for NT5E (see all 7) OriGene shRNA RFP: NT5E OriGene siRNA: NT5E
		QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat NT5E

Gene Editing
Products:

DNA2.0 Custom Protein Engineering Service for NT5E

Clone Products:		Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore
		OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for NT5E (see all 3) OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for NT5E OriGene custom cloning services � gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
		GenScript: all cDNA clones in your preferred vector (see all 2): NT5E (NM_002526)
		Sino Biological Human cDNA Clone for NT5E
		DNA2.0 Custom Codon Optimized Gene Synthesis Service for NT5E
		Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat NT5E

Cell Line Products:		GenScript Custom overexpressing Cell Line Services for NT5E
		Search LifeMap BioReagents cell lines for NT5E

In Situ Assay
Products:

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for NT5E

Gene Ontology (GO): 5 molecular function terms (GO ID links to tree view): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0000166	nucleotide binding	IEA	--
GO:0008198	ferrous iron binding	--	--
GO:0008253	5'-nucleotidase activity	EXP	--
GO:0016788	hydrolase activity, acting on ester bonds	--	--
GO:0046872	metal ion binding	IEA	--

NT5E for ontologies About GeneDecksing

2 GenomeRNAi human phenotypes for NT5E:

Decreased melanin production

Increased G2M DNA content

Animal Models:
Mouse knock-outs for NT5E: Nt5e^tm1Lft Nt5e^tm1Jsc Nt5e^tm1Jgsc
5 MGI mutant phenotypes (inferred from 3 alleles) (MGI details for Nt5e):

behavior/neurological

cardiovascular system

hematopoietic system

homeostasis/metabolism

renal/urinary system

NT5E for phenotypes About GeneDecksing

(Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to ¹UniProtKB, ²MINT, ³I2D, and/or ⁴STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
About This Section

Unified GeneCards pathways - 5/12 super-pathways (see all 12) About this table

See pathways by source

Super-pathway

contained gene-specific pathways

Purine metabolism

	Purine metabolism	1.00			Purine catabolism	0.30
	Metabolism of nucleotides	0.42

purine nucleotides degradation II (aerobic)

	purine nucleotides degradation II (aerobic)	1.00			urate biosynthesis/inosine 5-phosphate degradation	0.58
	adenosine nucleotides degradation II	0.67

NAD metabolism

	NAD metabolism	1.00			Nicotinate and nicotinamide metabolism	0.43
	NAD metabolism	1.00

Pyrimidine metabolism

Pyrimidine metabolism

1.00

Pyrimidine catabolism

0.50

Metabolism

Metabolism

1.00

Metabolic pathways

0.38

Pathway sources
See GeneCards unified pathways
Show all pathways

2 EMD Millipore Pathways for NT5E

	NAD metabolism
	ATP/ITP metabolism

2 Cell Signaling Technology (CST) Pathways for NT5E

	Neuroscience
	Lymphocyte Signaling

2 GeneGo (Thomson Reuters) Pathways for NT5E

	NAD metabolism
	ATP/ITP metabolism

5 BioSystems Pathways for NT5E

	adenosine nucleotides degradation II
	HIF-1-alpha transcription factor network
	NAD salvage pathway II
	urate biosynthesis/inosine 5-phosphate degradation
	purine nucleotides degradation II (aerobic)

5/6 Reactome Pathways for NT5E (see all 6)

	Metabolism of nucleotides
	Metabolism
	Purine catabolism
	Purine metabolism
	Pyrimidine catabolism

1 PharmGKB Pathway for NT5E

Thiopurine Pathway, Pharmacokinetics/Pharmacodynamics

4 Kegg Pathways (Kegg details for NT5E):

	Purine metabolism
	Pyrimidine metabolism
	Nicotinate and nicotinamide metabolism
	Metabolic pathways

NT5E for pathways About GeneDecksing

Interactions:

SABiosciences Gene Network Central^TM Interacting Genes and Proteins Network for NT5E

STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

5/85 Interacting proteins for NT5E (P21589³ ENSP00000257770⁴) via UniProtKB, MINT, STRING, and/or I2D (see all 85)

Interactant		Interaction Details
GeneCard	External ID(s)	Interaction Details
ACTB	P60709³, ENSP00000349960⁴	I2D: score=1 STRING: ENSP00000349960
FN1	P02751³, ENSP00000346839⁴	I2D: score=1 STRING: ENSP00000346839
LAMA1	P25391³, ENSP00000374309⁴	I2D: score=1 STRING: ENSP00000374309
AFF1	P51825³, ENSP00000305689⁴	I2D: score=1 STRING: ENSP00000305689
ADA	ENSP00000361965⁴	STRING: ENSP00000361965

About this table

Gene Ontology (GO): 5/13 biological process terms (GO ID links to tree view) (see all 13): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0006144	purine nucleobase metabolic process	TAS	--
GO:0006164	purine nucleotide biosynthetic process	--	--
GO:0006195	purine nucleotide catabolic process	TAS	--
GO:0006196	AMP catabolic process	IEA	--
GO:0006206	pyrimidine nucleobase metabolic process	TAS	--

NT5E for ontologies About GeneDecksing

(Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
About This Section

NT5E for compounds About GeneDecksing

Browse Small Molecules at EMD Millipore

Browse drugs & compounds from Enzo Life Sciences

Browse Tocris compounds for NT5E

2 DrugBank Compounds for NT5E About this table

Compound	Synonyms	CAS #	Type	Actions	PubMed Ids
Pentoxifylline	--	6493-05-6	target	inhibitor	11752352 16426349
Cytarabine	Ara-C (see all 26)	147-94-4	enzyme	substrate	19842938

10/24 Novoseek chemical compound relationships for NT5E gene (see all 24) About this table

Compound	-log (P-Val)	Hits	PubMed IDs for Articles with Shared Sentences (# sentences)
apcp	87.9	4	17487388 (2), 16644754 (1), 17671792 (1)
epsilon-amp	86.8	5	16418778 (1), 17065216 (1), 11046060 (1), 17471030 (1)
alpha,beta-methyleneadenosine-5'-diphosphate	83.6	1	17487388 (1)
purine	57	13	8566797 (2), 8906810 (2), 19253308 (2), 8224905 (1) (see all 8)
cyclic adp-ribose	42.9	7	15028729 (3), 8906810 (1)
nucleoside	39.9	9	16644754 (1), 18600539 (1), 16265592 (1), 15028729 (1) (see all 6)
adenylate	38.3	12	19729987 (2), 9169488 (2), 11046060 (2), 16861292 (1) (see all 7)
purine nucleoside	36.3	2	7655619 (1), 10343529 (1)
atp	34.3	13	19729987 (2), 19464286 (1), 19825957 (1), 16799190 (1) (see all 10)
adp	21.5	3	19464286 (1), 15319286 (1)

Search CenterWatch for drugs/clinical trials and news about NT5E / 5NTD

(Secondary structures according to fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from EMD Millipore,
siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
About This Section

REFSEQ mRNAs for NT5E gene (2 alternative transcripts):

NM_001204813.1 NM_002526.3

Unigene Cluster for NT5E:

5'-nucleotidase, ecto (CD73)

Hs.153952 [show with all ESTs]

Unigene Representative Sequence: NM_002526

5 Ensembl transcripts including schematic representations, and UCSC links where relevant:

ENST00000257770(uc003pko.4 uc010kbr.3) ENST00000369646(uc003pkn.3)

ENST00000416334 ENST00000437581 ENST00000369651

miRNA Products:		OriGene 3'-UTR Clone: NT5E Browse OriGene MicroRNA Expression Plasmids
		QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat NT5E
		8/19 QIAGEN miScript miRNA Assays for microRNAs that regulate NT5E (see all 19):
		hsa-miR-411* hsa-miR-30c hsa-miR-193a-3p hsa-miR-379* hsa-miR-657 hsa-miR-578 hsa-miR-30d hsa-miR-4260

		SwitchGear 3'UTR luciferase reporter plasmid: NT5E 3' UTR sequence

Inhib. RNA Products:		Browse for Gene Knock-down Tools from EMD Millipore
		OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for NT5E (see all 7) OriGene shRNA RFP: NT5E OriGene siRNA: NT5E
		QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat NT5E

Clone Products:		OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for NT5E (see all 3) OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for NT5E OriGene custom cloning services � gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
		GenScript: all cDNA clones in your preferred vector (see all 2): NT5E (NM_002526)
		DNA2.0 Custom Codon Optimized Gene Synthesis Service for NT5E
		Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat NT5E

Primer Products:		OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for NT5E Browse OriGene validated miRNA SYBR primer pairs
		SABiosciences RT² qPCR Primer Assay in human, mouse, rat NT5E
		QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat NT5E
		QIAGEN QuantiFast Probe-based Assays in human, mouse, rat NT5E

Additional cDNA sequence:

AK075008.1 AK075083.1 AK314661.1 BC015940.1 BC065937.1 DQ186653.1 X55740.1

18 DOTS entries:

DT.100819418 DT.101954345 DT.100819415 DT.100819421 DT.213229 DT.100819414 DT.100728243 DT.121330217

DT.121330067 DT.115575 DT.100819416 DT.92428164 DT.97806655 DT.100676979 DT.121330062 DT.92428162

DT.92428163 DT.75115257

24/269 AceView cDNA sequences (see all 269):

AA331558 AI371772 BP364913 BG747902 BF589378 BC015940 AA910593 BQ011191

H98118 AI935864 CB132912 AI989471 BE880721 BM761656 BM847311 CB130862

BQ010269 BX385710 CD106304 F01662 BG185851 W24986 W51864 AW006723

GeneLoc Exon Structure

3 Alternative Splicing Database (ASD) splice patterns (SP) for NT5E About this scheme

ExUns:	1a	·	1b	^	2	^	3a	·	3b	^	4	^	5	^	6	^	7	^	8	^	9
SP1:									-
SP2:									-								-
SP3:

ECgene alternative splicing isoforms for NT5E

(RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section

NT5E expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: AGCCTGCTCA

About this image

NT5E expression in embryonic tissues and stem cells

Expression by the Database of Embryonic development, Stem cell research, and Regenerative medicine About this table

5 LifeMap In Vivo Development Anatomical Compartments/Cells

Tissue

Anatomical Compartment

Cell

Category (developmental path)

Eye

Neuroblastic Layer

Early Photoreceptor Precursor Cells

Photoreceptors, Retina

Eye

Neuroblastic Layer

Late Photoreceptor Precursor Cells

Photoreceptors, Retina

Umbilical Cord

Wharton's Jelly

Umbilical Cord Mesenchymal Stem Cells

Extraembryonic Tissues, Umbilical Cord

Ovary

Ovary

Expression:

Positive

Negative

Selective marker

Experimental details:

Curated

Microarrays

In-situ hybridization

Stem Cell Differentiation: 10/11 LifeMap Cells (see all 11)

Name

Category

Placenta-derived mesenchymal stem cells (Fetal Stem / Progenitor Cell)

Adipose, Bone, Cartilage, Extraembryonic Tissues

Mesenchymal stem cell (Adult Stem / Progenitor Cell)

Bone

Placenta-derived adherent stromal cell (PLX) (Adult Stem / Progenitor Cell)

Extraembryonic Tissues, Placenta

Endometrial regenerative cell (Adult Stem / Progenitor Cell)

Reproductive System

PureStem™ progenitor SK17 (Embryonic Progenitor Cell)

Line H7 (WA07) (Embryonic Stem Cell)

Early Embryo, Inner Cell Mass

Mesenchymal Progenitor Cells - Passage 2 (Differentiation of ...)

Renal CD133+ cells (Renal progenitor (CD...)

Myofibroblasts/mesenchymal-like cells (Derivation of neural...)

CD73-positive cells (Generation of skelet...)

See NT5E Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for NT5E

SOURCE GeneReport for Unigene cluster: Hs.153952

SABiosciences Expression via Pathway-Focused PCR Arrays including NT5E:

Cell Surface Markers in human mouse rat

Mesenchymal Stem Cell in human mouse rat

Primer Products:		OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for NT5E Browse OriGene validated miRNA SYBR primer pairs
		SABiosciences RT² qPCR Primer Assay in human, mouse, rat NT5E
		QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat NT5E
		QIAGEN QuantiFast Probe-based Assays in human, mouse, rat NT5E

In Situ
Assay Products:

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for NT5E

(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD , ⁵MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or ⁶Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
About This Section

This gene was present in the last universal common ancestor (LUCA).

Orthologs for NT5E gene from 7/24 species (see all 24) About this table

Organism	Taxonomic classification	Gene	Description	Human Similarity	Orthology Type	Details
mouse (Mus musculus)	Mammalia	Nt5e^{1 , 5}	5' nucleotidase, ecto^{1, 5}	85.42(n)¹ 86.76(a)¹		9 (47.24 cM)⁵ 23959¹ NM_011851.4¹ NP_035981.1¹ 88327609⁵
chicken (Gallus gallus)	Aves	NT5E¹	5'-nucleotidase, ecto (CD73)	65.81(n) 65.01(a)		421833 XM_419855.3 XP_419855.2
lizard (Anolis carolinensis)	Reptilia	NT5E⁶ --	--	61(a) 60(a)	possible ortholog 1 ↔ 1	1(196519301-196533278) 1(176888687-176900867)
African clawed frog (Xenopus laevis)	Amphibia	Xl.14455²	Xenopus laevis transcribed sequence with moderate similarity more	76.69(n)		BJ042561.1
zebrafish (Danio rerio)	Actinopterygii	zgc63784²	similar to 5' nucleotidase, ecto	76.13(n)		393906 BC055243.1
fruit fly (Drosophila melanogaster)	Insecta	CG30104³ veil¹	5' nucleotidase precursor¹	39(a) (best of 4)³ 48.99(n)¹ 41.25(a)¹		36968¹ NM_137373.2¹ NP_611217.1¹
E. coli (Escherichia coli)	Gamma proteobacteria	ushA⁶	bifunctional UDP-sugar hydrolase/5'-nucleotidase	23(a)	1 ↔ 1	Chromosome(504138-505790)

ENSEMBL Gene Tree for NT5E (if available)
TreeFam Gene Tree for NT5E (if available)

(Paralogs according to ¹HomoloGene,
²Ensembl, and ³SIMAP, Pseudogenes according to ⁴Pseudogene.org Build 68)
About This Section

(SNPs/Variants according to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section

SNP ID	Valid	Clinical significance	Chr 6 pos	Sequence	#	AA Chg	Type	More	#	Allele freq	Pop	Total sample	More
Genomic Data					Transcription Related Data				Allele Frequencies
				--	--	--		--	--
rs111713497^1,2		--	83381623(+)	`AAAGCC/ACTGAT`	2	--	us2k¹		1		CSA	2
rs73496242^1,2	C,	--	83381796(+)	`TCGCCC/TACTGT`	2	--	us2k¹		2		WA	120
rs78126283^1,2	F,	--	83382349(+)	`GATCAG/ATTCCC`	2	--	us2k¹		1		WA	118
rs4463232^1,2	C,F,A,	--	83382448(+)	`aaacaT/Gccaca`	2	--	us2k¹		6		WA NA EA	364
rs9450278^1,2	C,F,A,H,	--	83382630(+)	`attggG/Atcctg`	2	--	us2k¹ tfbs³		20		NS EA NA WA CSA	2346
rs4599602^1,2	C,F,A,H,	--	83382706(+)	`agtttC/Tgtatc`	2	--	us2k¹ tfbs³		3		WA NA	6
rs71823414^1,2	C	--	83382718(+)	`CTGTT-/AAGTT TTTAG`	2	--	us2k¹		0	--	--	--	--
rs76973045^1,2	C,	--	83382739(+)	`TGGTTA/GTATAA`	2	--	us2k¹		2		WA	120
rs4458647^1,2	C,F,A,H,	--	83382793(+)	`cccttT/Cattac`	2	--	us2k¹		20		NS EA NA WA CSA	2344
rs76099537^1,2	F,	--	83383509(+)	`CAACCC/TAACAG`	2	--	us2k¹		1		WA	118

HapMap Linkage Disequilibrium report for NT5E (86159302 - 86205500 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV) variations for NT5E: --
Human Gene Mutation Database (HGMD): NT5E

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(in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
About This Section

NT5E for disorders           About GeneDecksing

OMIM gene information: 129190
OMIM disorders: 211800
UniProtKB/Swiss-Prot: 5NTD_HUMAN, P21589

Defects in NT5E are the cause of calcification of joints and arteries (CALJA) [MIM:211800]. A condition

characterized by adult-onset calcification of the lower extremity arteries, including the iliac, femoral and tibial

arteries, and hand and foot capsule joints. Age of onset has been reported as early as the second decade of life,

usually involving intense joint pain or calcification in the hands

20/44 diseases for NT5E (see all 44): About MalaCards

calcification of joints and arteries estrogen-receptor negative breast cancer pre-eclampsia beta thalassemia

chronic obstructive pulmonary disease haemophilus influenzae arterial calcification systemic lupus erythematosus

aortic aneurysm lupus erythematosus inflammatory bowel disease bronchial asthma

papillary thyroid carcinoma acute lymphoblastic leukemia chronic lymphocytic leukemia lymphoblastic leukemia

lymphocytic leukemia thalassemia periostitis varicocele

1 disease from the University of Copenhagen DISEASES database for NT5E:

Agammaglobulinemia

10/12 Novoseek disease relationships for NT5E gene (see all 12) About this table

Disease	-log (P-Val)	Hits	PubMed IDs for Articles with Shared Sentences (# sentences)
leukemia t-cell	30.5	2	8027539 (1), 2070084 (1)
zinc deficiency	20.4	3	1940572 (2), 9280142 (1)
inflammation	15.8	4	14734746 (1), 17911479 (1), 19149741 (1)
leukemia	0	8	2070084 (4), 8566797 (1)
primary tumor	0	2	2183704 (1), 17065075 (1)
breast cancer	0	18	17587186 (4), 17487388 (3), 17671792 (3), 17471030 (2) (see all 5)
metastasis	0	5	2183704 (1), 17065075 (1), 17587186 (1), 17471030 (1)
tumors	0	5	8566797 (2), 17487388 (2)
lymphoma	0	2	8566797 (1)
melanoma	0	5	17065075 (2), 16718268 (1)

Human Genome Epidemiology (HuGE) Navigator: NT5E (3 documents)

Export disorders for NT5E gene to outside databases

(in PubMed. Associations of this gene to articles via ¹Entrez Gene, ²UniProtKB/Swiss-Prot, ³HGNC, ⁴GAD, ⁵PharmGKB, ⁶HMDB, ⁷DrugBank, ⁸UniProtKB/TrEMBL, ⁹ Novoseek, and/or ¹⁰fRNAdb)
About This Section

PubMed articles for NT5E gene, integrated from 9 sources (see all 287):
(articles sorted by number of sources associating them with NT5E)

Utopia: connect your pdf to the dynamic
world of online information

Isolation and characterization of the promoter of the human 5'- nucleotidase (CD73)-encoding gene. (PubMed id 8566797)^{1, 2, 9} Hansen K.R.... Thompson L.F. (1995)
NT5E mutations and arterial calcifications. (PubMed id 21288095)^{1, 2} St Hilaire C.... Boehm M. (2011)
Signal sequence and keyword trap in silico for selection of full- length human cDNAs encoding secretion or membrane proteins from oligo- capped cDNA libraries. (PubMed id 16303743)^{1, 2} Otsuki T....Isogai T. (2005)
Primary structure of human placental 5'-nucleotidase and identification of the glycolipid anchor in the mature form. (PubMed id 2129526)^{1, 2} Misumi Y.... Ikehara Y. (1990)
Characterization of soluble vs membrane-bound human placental 5'- nucleotidase. (PubMed id 2173922)^{1, 2} Klemens M.R....Thompson L.F. (1990)
Differential regulation and function of CD73, a glycosyl-phosphatidylinositol-linked 70-kD adhesion molecule, on lymphocytes and endothelial cells. (PubMed id 9015312)^{1, 9} Airas L....Jalkanen S. (1997)
CD73 participates in cellular multiresistance program and protects against TRAIL-induced apoptosis. (PubMed id 18566412)^{1, 9} Mikhailov A....Eriksson J.E. (2008)
Regulation of endothelial CD73 by adenosine: paracrine pathway for enhanced endothelial barrier function. (PubMed id 11046060)^{1, 9} Narravula S....Colgan S.P. (2000)
Surface expression, polarization, and functional significance of CD73 in human intestinal epithelia. (PubMed id 9169488)^{1, 9} Strohmeier G.R....Madara J.L. (1997)
Ecto-enzyme and signaling functions of lymphocyte CD73. (PubMed id 9553767)^{1, 9} Resta R....Thompson L.F. (1998)

(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

		AND	OR
Aliases
Disorders
Free Text

Query String		PubMed OMIM NCBI Bookshelf
	(Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
About This Section

Entrez Gene: 4907	HGNC: 8021	AceView: NT5E	Ensembl:ENSG00000135318	euGenes: HUgn4907
ECgene: NT5E	Kegg: 4907	H-InvDB: NT5E

(According to HUGE)
About This Section

(According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

Name	Description
PharmGKB entry for NT5E	Pharmacogenomics, SNPs, Pathways

Intellectual Property for NT5E gene
(Patent information from GeneIP, Licensable technologies from WIS Yeda, Salk, Tufts, IP news from LifeMap Sciences, Inc.)

About This Section

Patent Information for NT5E gene:
Search GeneIP for patents involving NT5E

GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search

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5'-nucleotidase, ecto (CD73)

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5'-nucleotidase, ecto (CD73)

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