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Aliases for NT5C3B Gene

Aliases for NT5C3B Gene

  • 5'-Nucleotidase, Cytosolic IIIB 2
  • 5-Nucleotidase, Cytosolic IIIB 2 3 5
  • Cytosolic 5-Nucleotidase III-Like Protein 3 4
  • 5-Nucleotidase, Cytosolic III-Like 2 3
  • N(7)-Methylguanylate 5-Phosphatase 3 4
  • 7-Methylguanosine Nucleotidase 3 4
  • Cytosolic 5-Nucleotidase 3B 3 4
  • CN-III-Like Protein 3 4
  • NT5C3L 3 4
  • 7-Methylguanosine Phosphate-Specific 5-Nucleotidase 3
  • EC 3.1.3.91 4
  • EC 3.1.3.5 4
  • CN-IIIB 3

External Ids for NT5C3B Gene

Previous HGNC Symbols for NT5C3B Gene

  • NT5C3L

Previous GeneCards Identifiers for NT5C3B Gene

  • GC17M039982

Summaries for NT5C3B Gene

GeneCards Summary for NT5C3B Gene

NT5C3B (5'-Nucleotidase, Cytosolic IIIB) is a Protein Coding gene. Among its related pathways are NAD metabolism and Deadenylation-dependent mRNA decay. GO annotations related to this gene include nucleotide binding and 5-nucleotidase activity. An important paralog of this gene is NT5C3A.

UniProtKB/Swiss-Prot for NT5C3B Gene

  • Specifically hydrolyzes 7-methylguanosine monophosphate (m(7)GMP) to 7-methylguanosine and inorganic phosphate (PubMed:23223233, PubMed:24603684). The specific activity for m(7)GMP may protect cells against undesired salvage of m(7)GMP and its incorporation into nucleic acids (PubMed:23223233). Also has weak activity for CMP (PubMed:23223233, PubMed:24603684). UMP and purine nucleotides are poor substrates (PubMed:23223233).

No data available for Entrez Gene Summary , CIViC summary , Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for NT5C3B Gene

Genomics for NT5C3B Gene

Regulatory Elements for NT5C3B Gene

Enhancers for NT5C3B Gene
GeneHancer Identifier Enhancer Score Enhancer Sources Gene-Enhancer Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor Binding Sites within enhancer Gene Targets for Enhancer
GH17G041929 1.4 ENCODE dbSUPER 67.7 -94.0 -93989 2.4 HDGF PKNOX1 FOXA2 MLX CREB3L1 ARNT WRNIP1 SIN3A ARID4B DMAP1 NT5C3B CCR10 KAT2A HSD17B1P1 ENSG00000259623 CNTNAP1 HSD17B1 ZNF385C ENSG00000267765 LOC108783654
GH17G041913 1.5 Ensembl ENCODE dbSUPER 61.1 -78.5 -78493 2.9 HDGF FOXA2 ARID4B SIN3A DMAP1 FEZF1 YY1 GLIS2 ZNF263 SP5 NT5C3B GC17P041919 GC17M041900 PIR36467
GH17G041962 1.5 Ensembl ENCODE dbSUPER 60 -126.9 -126882 1.1 PKNOX1 FOXA2 ARNT ARID4B DMAP1 ZNF2 YY1 ZNF121 GLIS2 ZNF143 NT5C3B CNP GC17M041929
GH17G041894 1.1 ENCODE dbSUPER 79.3 -59.4 -59400 2.7 CTCF ZBTB21 JUN KLF17 CEBPG RAD21 ZEB1 ZNF324 ZNF366 ZNF600 NT5C3B LOC108783654 KLHL11 KLHL10 ENSG00000267758 GAST CNP FKBP10 PIR36467 GC17M041900
GH17G041835 0.9 ENCODE 93.3 +0.2 239 1.6 SIN3A ARID4B DMAP1 GLI4 ZNF48 SLC30A9 GLIS2 PAF1 REST KAT8 NT5C3B KLHL10
- Elite enhancer and/or Elite enhancer-gene association Download GeneHancer data dump

Enhancers around NT5C3B on UCSC Golden Path with GeneCards custom track

Promoters for NT5C3B Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters
ENSR00000094297 271 1201 HDAC1 RB1 SIN3A ARID4B DMAP1 GLI4 ZNF48 SLC30A9 GLIS2 EGR1

Genomic Location for NT5C3B Gene

Chromosome:
17
Start:
41,825,082 bp from pter
End:
41,836,271 bp from pter
Size:
11,190 bases
Orientation:
Minus strand

Genomic View for NT5C3B Gene

Genes around NT5C3B on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
NT5C3B Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for NT5C3B Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for NT5C3B Gene

Proteins for NT5C3B Gene

  • Protein details for NT5C3B Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q969T7-5NT3B_HUMAN
    Recommended name:
    7-methylguanosine phosphate-specific 5-nucleotidase
    Protein Accession:
    Q969T7
    Secondary Accessions:
    • A8MWB9
    • C9JKC4
    • Q7L3B7

    Protein attributes for NT5C3B Gene

    Size:
    300 amino acids
    Molecular mass:
    34389 Da
    Quaternary structure:
    • Monomer.

    Alternative splice isoforms for NT5C3B Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for NT5C3B Gene

Post-translational modifications for NT5C3B Gene

  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

No data available for DME Specific Peptides for NT5C3B Gene

Domains & Families for NT5C3B Gene

Protein Domains for NT5C3B Gene

Suggested Antigen Peptide Sequences for NT5C3B Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

Q969T7

UniProtKB/Swiss-Prot:

5NT3B_HUMAN :
  • Belongs to the pyrimidine 5-nucleotidase family.
Family:
  • Belongs to the pyrimidine 5-nucleotidase family.
genes like me logo Genes that share domains with NT5C3B: view

No data available for Gene Families for NT5C3B Gene

Function for NT5C3B Gene

Molecular function for NT5C3B Gene

UniProtKB/Swiss-Prot BiophysicochemicalProperties:
Kinetic parameters: KM=7.8 uM for m(7)GMP (at 37 degrees Celsius) {ECO:0000269 PubMed:23223233}; KM=8 uM for m(7)GMP (at 37 degrees Celsius) {ECO:0000269 PubMed:24603684}; KM=79 uM for CMP (at 37 degrees Celsius) {ECO:0000269 PubMed:23223233, ECO:0000269 PubMed:24603684}; KM=355 uM for GMP (at 37 degrees Celsius) {ECO:0000269 PubMed:23223233}; KM=456 uM for AMP (at 37 degrees Celsius) {ECO:0000269 PubMed:23223233}; KM=439 uM for UMP (at 37 degrees Celsius) {ECO:0000269 PubMed:23223233}; Vmax=0.41 umol/min/mg enzyme with m(7)GMP as substrate {ECO:0000269 PubMed:23223233}; Vmax=12 umol/min/mg enzyme with CMP as substrate {ECO:0000269 PubMed:23223233}; Vmax=0.13 umol/min/mg enzyme with GMP as substrate {ECO:0000269 PubMed:23223233}; Vmax=0.07 umol/min/mg enzyme with AMP as substrate {ECO:0000269 PubMed:23223233}; Vmax=10.7 umol/min/mg enzyme with UMP as substrate {ECO:0000269 PubMed:23223233}; Note=kcat is 0.24 sec(-1) with m(7)GMP. kcat is 7 sec(-1) with CMP. kcat is 0.07 sec(-1) with GMP. kcat is 0.04 sec(-1) with AMP. kcat is 6.2 sec(-1) with UMP.;
UniProtKB/Swiss-Prot CatalyticActivity:
N(7)-methyl-GMP + H(2)O = N(7)-methyl-guanosine + phosphate.
UniProtKB/Swiss-Prot CatalyticActivity:
CMP + H(2)O = cytidine + phosphate.
UniProtKB/Swiss-Prot CatalyticActivity:
A 5-ribonucleotide + H(2)O = a ribonucleoside + phosphate.
UniProtKB/Swiss-Prot Function:
Specifically hydrolyzes 7-methylguanosine monophosphate (m(7)GMP) to 7-methylguanosine and inorganic phosphate (PubMed:23223233, PubMed:24603684). The specific activity for m(7)GMP may protect cells against undesired salvage of m(7)GMP and its incorporation into nucleic acids (PubMed:23223233). Also has weak activity for CMP (PubMed:23223233, PubMed:24603684). UMP and purine nucleotides are poor substrates (PubMed:23223233).

Enzyme Numbers (IUBMB) for NT5C3B Gene

Gene Ontology (GO) - Molecular Function for NT5C3B Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000166 nucleotide binding IEA --
GO:0000287 magnesium ion binding IEA --
GO:0005515 protein binding IPI 20706999
GO:0008253 5-nucleotidase activity TAS,IEA --
GO:0016787 hydrolase activity IEA --
genes like me logo Genes that share ontologies with NT5C3B: view
genes like me logo Genes that share phenotypes with NT5C3B: view

Animal Model Products

CRISPR Products

miRNA for NT5C3B Gene

miRTarBase miRNAs that target NT5C3B

Inhibitory RNA Products

Clone Products

No data available for Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for NT5C3B Gene

Localization for NT5C3B Gene

Subcellular locations from UniProtKB/Swiss-Prot for NT5C3B Gene

Cytoplasm.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for NT5C3B gene
Compartment Confidence
cytosol 5
nucleus 3
cytoskeleton 2
extracellular 1
peroxisome 1

Gene Ontology (GO) - Cellular Components for NT5C3B Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005737 cytoplasm IEA --
GO:0005829 cytosol TAS --
genes like me logo Genes that share ontologies with NT5C3B: view

Pathways & Interactions for NT5C3B Gene

genes like me logo Genes that share pathways with NT5C3B: view

Interacting Proteins for NT5C3B Gene

Gene Ontology (GO) - Biological Process for NT5C3B Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0009117 nucleotide metabolic process IEA --
GO:0016311 dephosphorylation IEA --
GO:0043928 exonucleolytic nuclear-transcribed mRNA catabolic process involved in deadenylation-dependent decay TAS --
genes like me logo Genes that share ontologies with NT5C3B: view

No data available for SIGNOR curated interactions for NT5C3B Gene

Drugs & Compounds for NT5C3B Gene

(3) Drugs for NT5C3B Gene - From: HMDB

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Phosphoric acid Approved Pharma 0
Water Approved Pharma 0
Ergocalciferol Approved Nutra 1281
genes like me logo Genes that share compounds with NT5C3B: view

Transcripts for NT5C3B Gene

Unigene Clusters for NT5C3B Gene

5-nucleotidase, cytosolic IIIB:
Representative Sequences:

CRISPR Products

Inhibitory RNA Products

Clone Products

Alternative Splicing Database (ASD) splice patterns (SP) for NT5C3B Gene

ExUns: 1a · 1b · 1c · 1d · 1e ^ 2 ^ 3 ^ 4a · 4b · 4c ^ 5 ^ 6 ^ 7a · 7b ^ 8 ^ 9 ^ 10a · 10b · 10c ^ 11 ^ 12a · 12b
SP1: - - - - - -
SP2: - - - - - -
SP3: - - -
SP4: - - - - - - - -
SP5: - - - - - -
SP6: -
SP7: - - - -
SP8: - - - - - - -
SP9: - - - - - - - -
SP10: - - - - -
SP11: - -
SP12: - -
SP13:

Relevant External Links for NT5C3B Gene

GeneLoc Exon Structure for
NT5C3B
ECgene alternative splicing isoforms for
NT5C3B

Expression for NT5C3B Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and CGAP SAGE for NT5C3B Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Protein differential expression in normal tissues from HIPED for NT5C3B Gene

This gene is overexpressed in Skin (16.7), Uterus (8.2), and Salivary gland (7.7).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MaxQB, and MOPED for NT5C3B Gene



NURSA nuclear receptor signaling pathways regulating expression of NT5C3B Gene:

NT5C3B

SOURCE GeneReport for Unigene cluster for NT5C3B Gene:

Hs.237536

Evidence on tissue expression from TISSUES for NT5C3B Gene

  • Liver(4.2)
  • Lung(4.2)
genes like me logo Genes that share expression patterns with NT5C3B: view

Primer Products

No data available for mRNA differential expression in normal tissues , Protein tissue co-expression partners , mRNA Expression by UniProt/SwissProt and Phenotype-based relationships between genes and organs from Gene ORGANizer for NT5C3B Gene

Orthologs for NT5C3B Gene

This gene was present in the common ancestor of eukaryotes.

Orthologs for NT5C3B Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia NT5C3L 34
  • 99.67 (n)
NT5C3B 35
  • 99 (a)
OneToOne
dog
(Canis familiaris)
Mammalia NT5C3B 34 35
  • 91.53 (n)
cow
(Bos Taurus)
Mammalia NT5C3L 35 34
  • 90.08 (n)
OneToOne
rat
(Rattus norvegicus)
Mammalia Nt5c3b 34
  • 87.85 (n)
mouse
(Mus musculus)
Mammalia Nt5c3b 34 16 35
  • 87.29 (n)
oppossum
(Monodelphis domestica)
Mammalia NT5C3B 35
  • 62 (a)
OneToOne
chicken
(Gallus gallus)
Aves NT5C3L 34
  • 69.11 (n)
NT5C3B 35
  • 65 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia NT5C3B 35
  • 64 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia nt5c3b 34
  • 68.41 (n)
Str.14210 34
African clawed frog
(Xenopus laevis)
Amphibia LOC398553 34
fruit fly
(Drosophila melanogaster)
Insecta CG3362 34 35
  • 52.36 (n)
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP010681 34
  • 51.5 (n)
worm
(Caenorhabditis elegans)
Secernentea Y10G11A.1 35
  • 36 (a)
ManyToMany
F25B5.3 35
  • 28 (a)
ManyToMany
thale cress
(Arabidopsis thaliana)
eudicotyledons AT2G38680 34
  • 48.5 (n)
rice
(Oryza sativa)
Liliopsida Os03g0648900 34
  • 49.49 (n)
sea squirt
(Ciona savignyi)
Ascidiacea CSA.3144 35
  • 45 (a)
OneToMany
Species where no ortholog for NT5C3B was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • platypus (Ornithorhynchus anatinus)
  • rainbow trout (Oncorhynchus mykiss)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • zebrafish (Danio rerio)

Evolution for NT5C3B Gene

ENSEMBL:
Gene Tree for NT5C3B (if available)
TreeFam:
Gene Tree for NT5C3B (if available)

Paralogs for NT5C3B Gene

Paralogs for NT5C3B Gene

(1) SIMAP similar genes for NT5C3B Gene using alignment to 6 proteins:

genes like me logo Genes that share paralogs with NT5C3B: view

Variants for NT5C3B Gene

Sequence variations from dbSNP and Humsavar for NT5C3B Gene

SNP ID Clin Chr 17 pos Sequence Context AA Info Type
rs1000083147 -- 41,826,453(+) CCTGG(C/G)CTCAA intron-variant
rs1000309808 -- 41,829,451(+) TTGAC(A/G)TATGT intron-variant
rs1000409948 -- 41,829,930(+) AGGGT(A/G)CAAAC intron-variant
rs1000426034 -- 41,836,028(+) TCGCT(C/T)GGGCG intron-variant, nc-transcript-variant, upstream-variant-2KB
rs1001145255 -- 41,828,374(+) ACTCT(A/G)TCACC intron-variant

Structural Variations from Database of Genomic Variants (DGV) for NT5C3B Gene

Variant ID Type Subtype PubMed ID
dgv3174n100 CNV gain 25217958
nsv1057008 CNV gain 25217958
nsv1146669 OTHER inversion 26484159
nsv827994 CNV gain 20364138
nsv833447 CNV loss 17160897

Variation tolerance for NT5C3B Gene

Residual Variation Intolerance Score: 38.8% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 1.11; 22.56% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for NT5C3B Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
NT5C3B

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for NT5C3B Gene

Disorders for NT5C3B Gene

Relevant External Links for NT5C3B

Atlas of Genetics and Cytogenetics in Oncology and Haematology:
NT5C3B

No disorders were found for NT5C3B Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for NT5C3B Gene

Publications for NT5C3B Gene

  1. Crystal structures of the novel cytosolic 5'-nucleotidase IIIB explain its preference for m7GMP. (PMID: 24603684) Monecke T. … Ficner R. (PLoS ONE 2014) 2 3 4 64
  2. Identification of Drosophila and Human 7-Methyl GMP-specific Nucleotidases. (PMID: 23223233) Buschmann J. … Wahle E. (J. Biol. Chem. 2013) 2 3 4 64
  3. Novel genes for airway wall thickness identified with combined genome-wide association and expression analyses. (PMID: 25517131) Dijkstra A.E. … Groen H.J. (Am. J. Respir. Crit. Care Med. 2015) 3 64
  4. Insights into RNA biology from an atlas of mammalian mRNA-binding proteins. (PMID: 22658674) Castello A. … Hentze M.W. (Cell 2012) 3 64
  5. Initial characterization of the human central proteome. (PMID: 21269460) Burkard T.R. … Colinge J. (BMC Syst. Biol. 2011) 4 64

Products for NT5C3B Gene

Sources for NT5C3B Gene

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