NSG1 Gene
protein-coding GIFtS: 38
GCID: GC04P004350
|
|
NSG1
| |
Aliases
for NSG1 gene
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc,
7Ensembl,
8DME,
9miRBase,
and/or 10fRNAdb)
About This Section
|
| Aliases |
|---|
| D4S2342 3 5 | | P212 | | Brain Neuron Cytoplasmic Protein 12 3 | | Carboxyterminally EE-Tagged Neuron-Enriched Endosomal 21 KDa Protein2 | | D4S234E2 5 | | Neuron Specific Gene Family Member 12 | | NEEP212 | | Neuron-Specific Protein Family Member 12 |
Export aliases for NSG1 gene to outside databases |
Summaries
for NSG1 gene(According to Entrez Gene,
Tocris Bioscience,
Wikipedia's
Gene Wiki,
PharmGKB,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
|
-- |
Genomic Views
for NSG1 gene
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to
UCSC (hg19) and
Ensembl (release 69),
Regulatory elements and Epigenetics data according to
QIAGEN,
SABiosciences, and/or
SwitchGear Genomics)
About This Section
| RefSeq DNA sequence:- NC_000004.11 NC_018915.1 NT_006051.18
Regulatory elements:
 Search SABiosciences Regulatory transcription factor binding sites for NSG1
Other transcription factors
Search SABiosciences Chromatin IP Primers for NSG1
Epigenetics:
| | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat NSG1 |
Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track
Entrez Gene cytogenetic band: 4p16.3 Ensembl cytogenetic band: 4p16.3 NSG1 Gene in genomic location: bands according to Ensembl, locations according to
 (and/or Entrez Gene and/or Ensembl if different)
GeneLoc information about chromosome 4 GeneLoc Exon Structure GeneLoc location for GC04P004350: view genomic region
(about GC identifiers)
Start:
|
4,349,867 bp from pter |
End:
|
4,420,785 bp from pter |
Size:
|
70,919 bases |
Orientation:
|
plus strand |
|
Proteins
for NSG1 gene
(According to
1UniProtKB,
HORDE,
neXtProt,
Ensembl,
and/or Reactome,
Modification sites according to 2PhosphoSitePlus,
Specific Peptides from DME,
Protein expression images according to data from SPIRE MOPED and PaxDb,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from
EMD Millipore,
R&D Systems,
GenScript,
Enzo Life Sciences,
OriGene,
Novus Biologicals,
Sino Biological,
ProSpec, and/or
Uscn,
Biochemical Assays by
EMD Millipore,
R&D Systems,
OriGene,
GenScript,
Cell Signaling Technology,
Enzo Life Sciences, and/or
Uscn,
Ontologies according to Gene
Ontology Consortium 01 Mar 2013 and
Entrez Gene,
Antibodies by
EMD Millipore,
R&D Systems,
GenScript,
Cell Signaling Technology,
OriGene,
Novus Biologicals,
Thermo Fisher Scientific,
Abcam, and/or
Uscn)
About This Section
|
UniProtKB/Swiss-Prot: NSG1_HUMAN, P42857 (See
protein sequence)Recommended Name: Neuron-specific protein family member 1 Size: 185 amino acids; 20913 Da
Subcellular location: Golgi apparatus membrane; Single-pass type II membrane protein (Potential)
Secondary accessions: Q49AQ1Explore the universe of human proteins at neXtProt for NSG1: NX_P42857
Post-translational modifications:
View modification sites using PhosphoSitePlus2View neXtProt modification sites for NX_P42857
NSG1 Protein expression data from MOPED and PaxDb: About this image 
REFSEQ proteins (2 alternative transcripts):
NP_001035190.1 NP_055207.1
ENSEMBL proteins: ENSP00000388823 ENSP00000426358 ENSP00000421841 ENSP00000425423 ENSP00000408833 ENSP00000425345 ENSP00000425803 ENSP00000381049
Human Recombinant Protein Products:
Gene Ontology (GO): 4 cellular component terms (GO ID links to tree view): About this table
NSG1 for ontologies About GeneDecksing
NSG1 Antibody Products:
Assay Products for NSG1: |
Protein
Domains /
Families
for NSG1 gene(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
|
NSG1 for domains About GeneDecksing
1 InterPro domain/family:Graphical View of Domain Structure for InterPro Entry P42857ProtoNet protein and cluster: P42857 1 Blocks protein family: IPB009431 D1 dopamine receptor-interacting
UniProtKB/Swiss-Prot: NSG1_HUMAN, P42857Similarity: Belongs to the NSG family |
Function
for NSG1 gene
(According to 1UniProtKB,
Genatlas,
LifeMap Discovery™,
IUBMB, and/or
2DME,
Human phenotypes from GenomeRNAi,
Animal models from MGI Mar 06 2013,
bound targets from SABiosciences,
miRNA Gene Targets from miRTarBase
shRNA from
OriGene,
RNAi from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
microRNA from QIAGEN,
Gene Editing from DNA2.0,
Clones from EMD Millipore,
OriGene,
SwitchGear Genomics,
GenScript,
Sino Biological,
DNA2.0,
and Vector BioLabs,
Cell Lines from GenScript,
LifeMap BioReagents,
In Situ Hybridization Assays from Advanced Cell Diagnostics,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene.)
About This Section
|
Clone
Products: |  | Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore | |  | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for NSG1 (see all 4)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for NSG1 (see all 2)
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling 
| |  | GenScript Custom all cDNA clones Services for NSG1 | |  | Browse Sino Biological Human cDNA Clones | |  | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for NSG1 | |  | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat NSG1 |
In Situ Assay
Products: |
 | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for NSG1 |
Gene Ontology (GO): 1 molecular function term (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0050780 | dopamine receptor binding |
IEA | -- |
NSG1 for ontologies About GeneDecksing
1 GenomeRNAi human phenotype for NSG1:
|
Pathways & Interactions
for NSG1 gene
(Pathways according to
EMD Millipore,
R&D Systems,
Cell Signaling Technology,
KEGG,
PharmGKB,
BioSystems,
Reactome,
Tocris Bioscience,
GeneGo (Thomson Reuters),
QIAGEN,
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Interaction Networks according to
SABiosciences,
and/or STRING,
Interactions according to 1UniProtKB,
2MINT,
3I2D, and/or
4STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene).
About This Section
|
Interactions:
Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for NSG1
STRING Interaction
Network Preview (showing 1 interactants - click image to see more details)
2 Interacting proteins for NSG1 (P428573 ENSP000003810494) via UniProtKB, MINT, STRING, and/or I2DAbout this table
Gene Ontology (GO): 1 biological process term (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0007212 | dopamine receptor signaling pathway |
IEA | -- |
NSG1 for ontologies About GeneDecksing
|
Drugs & Compounds
for NSG1 gene(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
EMD Millipore, Tocris Bioscience
HMDB,
BitterDB, and/or
Novoseek, and Drugs according to
DrugBank,
Enzo Life Sciences, and/or
PharmGKB, with drugs/clinical trials/news
search links to CenterWatch)
About This Section
|
 Browse Tocris compounds for NSG1
Search CenterWatch for drugs/clinical trials and news about NSG1
|
Transcripts
for NSG1 gene(Secondary structures according to
fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
transcript ids from Ensembl
with links to UCSC,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
shRNA from
OriGene,
microRNA from QIAGEN,
Tagged/untagged cDNA clones from
OriGene,
SwitchGear Genomics,
GenScript,
DNA2.0,
Vector BioLabs,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN
)
About This Section
|
REFSEQ mRNAs for NSG1 gene (2 alternative transcripts): NM_001040101.1 NM_014392.3 Unigene Cluster for NSG1: Neuron specific gene family member 1 Hs.518595 [show with all ESTs]Unigene Representative Sequence: BC03422710 Ensembl transcripts including schematic representations, and UCSC links where relevant: ENST00000421177(uc011bvz.2 uc011bwa.1 uc003ghz.3) ENST00000513555(uc003gia.3)
ENST00000450293 ENST00000504347 ENST00000505246 ENST00000506380 ENST00000433139
ENST00000513829 ENST00000504171 ENST00000397958
Clone
Products: | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for NSG1 (see all 4)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for NSG1 (see all 2)
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling
| | | GenScript Custom all cDNA clones Services for NSG1 | | | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for NSG1 | | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat NSG1 |
Additional cDNA sequence: AK094573.1 AK125172.1 AK299657.1 AK301911.1 AK312288.1 BC034227.1 18 DOTS entries: DT.100833829 DT.313727 DT.97841936 DT.95282827 DT.100833826 DT.100706055 DT.100833819 DT.95369500 DT.100759509 DT.121258236 DT.121258246 DT.100833813 DT.101984578 DT.121258215 DT.121258264 DT.121258311 DT.121258381 DT.97787107 1 AceView cDNA sequence: AK096645
GeneLoc Exon Structure
5/9 Alternative Splicing Database (ASD) splice patterns (SP) for NSG1 (see all 9) About this scheme
| ExUns: | 1 | ^ | 2a | · | 2b | ^ | 3a | · | 3b | ^ | 4a | · | 4b | · | 4c | · | 4d | ^ | 5a | · | 5b | ^ | 6 | ^ | 7a | · | 7b | ^ | 8 | ^ | 9a | · | 9b | · | 9c | ^ | 10a | · | 10b | · | 10c | · | 10d | · | 10e | · | 10f | · | 10g |
|---|
|
| SP1: | | | - | | | | - | | - | | - | | - | | - | | - | | | | | | - | | | | | | - | | | | | | | | | | | | | | | | | | | | | | |
| SP2: | | | | | | | - | | - | | - | | - | | - | | | | | | | | - | | | | | | - | | | | | | | | | | | | | | | | | | | | | | |
| SP3: | | | | | | | | | | | - | | - | | - | | | | | | | | - | | | | | | - | | | | | | | | | | | | | | | | | | | | | | |
| SP4: | | | | | | | | | | | - | | - | | - | | - | | | | | | - | | | | | | - | | | | | | | | | | | | | | | | | | | | | | |
| SP5: | | | | | | | | | | | | | | | | | | | | | | | - | | | | | | - | | | | | | | | | | | | | | | | | | | | | |
|
Expression
for NSG1 gene
(RNA expression data according to
H-InvDB,
NONCODE,
miRBase, and
RNAdb,
Expression images according to data from
BioGPS,
Illumina Human BodyMap, and
CGAP
SAGE,
Sets of similar genes according to GeneDecks,
in vivo and in vitro expression data from LifeMap Discovery™,
plus additional links to
Genevestigator, and/or
SOURCE, and/or
BioGPS, and/or
UniProtKB,
PCR Arrays from
SABiosciences,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN,
In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section
| See probesets specificity/sensitivity at GeneAnnot
CGAP TAG: --
See NSG1 Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for NSG1
SOURCE GeneReport for Unigene cluster: Hs.518595
SABiosciences Custom PCR Arrays for NSG1
Primer
Products: | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for NSG1
Browse OriGene validated miRNA SYBR primer pairs
| | | Search SABiosciences RT2 qPCR
Primer Assays in human, mouse, rat NSG1 | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat NSG1 | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat NSG1 | In Situ
Assay Products: |
| Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for NSG1 |
Orthologs
for NSG1 gene
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
,
5MGI Mar 06 2013,
with possible further links to
Flybase
and/or
WormBase,
and/or
6Ensembl pan taxonomic compara ,
Gene Trees according to Ensembl and
TreeFam)
About This Section
|
This gene was present in the common ancestor of chordates.
Orthologs for NSG1 gene from 4/12 species (see all 12) About this table
| Organism |
Taxonomic
classification |
Gene |
Description |
Human
Similarity |
Orthology
Type |
Details |
chicken
(Gallus gallus) |
Aves |
NSG11 |
neuron specific gene family member 1 |
81.44(n)
88.65(a) |
|
395251 NM_204565.1 NP_989896.1 |
lizard
(Anolis carolinensis) |
Reptilia |
-- |
-- |
86(a) |
1 ↔ 1 |
GL343236.1(700014-729216) |
African clawed frog
(Xenopus laevis) |
Amphibia |
Xl.181462 |
Xenopus laevis transcribed sequence with moderate similarity more |
74.74(n) |
|
CF547856.1 |
rainbow trout
(Oncorhynchus mykiss) |
Actinopterygii |
Omy.45292 |
Oncorhynchus mykiss transcribed sequence with weak more |
75.59(n) |
|
CA373479.1 |
ENSEMBL Gene Tree for NSG1 (if available)
TreeFam Gene Tree for NSG1 (if available) |
Paralogs
for NSG1 gene(Paralogs according to
1HomoloGene,
2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
About This Section
| Paralogs for NSG1 gene
- HMP192 CALY2
4 SIMAP similar genes for NSG1 using alignment to 2 protein entries: NSG1_HUMAN (see all proteins):D4S234E HMP19 NSG2 CALY
NSG1 for paralogs About GeneDecksing
|
Genomic Variants
for NSG1 gene(SNPs/Variants according to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE,
UniProtKB, and
DNA2.0,
Linkage Disequilibrium by HapMap,
Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene
Mutation Database (HGMD) and the Locus Specific Mutation
Databases (LSDB), Blood group antigen gene mutations by BGMUT,
Resequencing Primers from QIAGEN,
Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section
|
| Genomic Data | Transcription Related Data | Allele Frequencies | | SNP ID | Valid | Clinical
significance | Chr 4 pos | Sequence | # | AA
Chg | Type | More | # | Allele
freq | Pop | Total
sample | More |
|---|
HapMap Linkage Disequilibrium report for NSG1 (4349867 - 4420785 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV) variations for NSG1: -- | SABiosciences Cancer Mutation PCR Assays |
| | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing NSG1 |
|
Disorders
/ Diseases
for NSG1 gene
(in which this Gene is Involved, According to MalaCards,
OMIM, UniProtKB,
the University of Copenhagen DISEASES
database,
Genatlas, GeneTests,
GAD,
HuGE Navigator,
and/or TGDB.)
About This Section
|
NSG1 for disorders About GeneDecksing
OMIM gene information: 607645
OMIM disorders: --
8  diseases for NSG1: About MalaCardsneuronitis long qt syndrome acute lymphoblastic leukemia multiple system atrophy
lymphoblastic leukemia leiomyoma neuroblastoma leukemia
Export disorders for NSG1 gene to outside databases
|
Publications
for NSG1 gene (in
PubMed.
Associations of this gene to articles via
1Entrez Gene,
2UniProtKB/Swiss-Prot,
3HGNC,
4GAD,
5PharmGKB,
6HMDB,
7DrugBank,
8UniProtKB/TrEMBL,
9 Novoseek, and/or
10fRNAdb)
About This Section
|
PubMed articles for NSG1 gene, integrated from 9 sources (see all 17):
(articles sorted by number of sources associating them with NSG1) | |  | Utopia: connect your pdf to the dynamic
world of online information |
- The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
- Variable subcellular localization of a neuron-specific protein during NTera 2 differentiation into post-mitotic human neurons. (PubMed id 9013775)1, 2 Carlock L....Dunbar J.C. (1996)
- Identification of NEEP21, encoding neuron-enriched en dosomal protein of 21 kDa, as a transcriptional target of tumor suppressor p53. (PubMed id 20878061)1 Ohnishi S....Arakawa H. (2010)
- Identification of NEEP21 as a A9-amyloid precursor pro tein-interacting protein in vivo that modulates amyloidogenic processing in vit ro. (PubMed id 21084623)1 Norstrom E.M....Sisodia S.S. (2010)
- D4S234E, a novel p53-responsive gene, induces apoptos is in response to DNA damage. (PubMed id 20599942)1 Kudoh T....Yoshida K. (2010)
- Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1 Ota T.... Sugano S. (2004)
- Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)1 Strausberg R.L....Marra M.A. (2002)
- Modulation of receptor cycling by neuron-enriched endosomal protein of 21 kD. (PubMed id 12070131)1 Steiner P....Hirling H. (2002)
- The endosomal protein NEEP21 regulates AMPA receptor-mediated synaptic transmission and plasticity in the hippocampus. (PubMed id 15911354)9 Alberi S....Muller D. (2005)
- The p21 codon 31*C- and DRD2 codon 313*T-related genotypes/alleles, but not XRCC1 codon 399, hOGG1 codon 326, and DRD1-48 polymorphisms, are correlated with the presence of leiomyoma. (PubMed id 19176223)9 Hsieh Y.Y....Chen C.P. (2009)
|
External Searches for NSG1 gene
(in PubMed,
OMIM, and NCBI Bookshelf)
About This Section
|
|
Genome Databases showing NSG1 gene
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
Kegg,
and/or
H-InvDB)
About This Section
|
|
Other Databases showing NSG1 gene
(According to HUGE)
About This Section
| -- |
Specialized Databases showing NSG1 gene(According to PharmGKB,
ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section
|
|
About This Section
| Patent Information for NSG1 gene:
Search GeneIP for patents involving NSG1
GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search
|
Products
for NSG1 gene(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or
Enzo Life Sciences),
In Situ Hybridization Assays from
Advanced Cell Diagnostics
About This Section
|
 | |
 | |
 |
| | |  | Browse OriGene Antibodies | | OriGene shRNA RFP for NSG1 | | | OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for NSG1 | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for NSG1 | | | OriGene Protein Over-expression Lysate for NSG1 | | Browse OriGene Fluorogenic Cell Assay Kits | | | OriGene siRNA for NSG1 | | OriGene 3'-UTR Clone for NSG1 | | | OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for NSG1 | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for NSG1 | | | Browse OriGene GFP tagged cDNA clones in CMV expression vector | | Browse OriGene MicroRNA Expression Plasmids | | | Browse OriGene basic RS shRNAs | | Browse OriGene validated miRNA SYBR primer pairs | | | OriGene Purified Protein for NSG1 | | OriGene custom cloning services - gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling | | | OriGene Custom Antibody Services for NSG1 | | OriGene Custom Protein Services for NSG1 | | | OriGene Custom Immunoassay Development | | |
| |
 |
 |
| | | QIAGEN Custom miScript Target Protector blocks miRNA-binding site of in human, mouse, rat NSG1 | | | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing NSG1 | | | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat NSG1 | | | QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat NSG1 | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat NSG1 | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat NSG1 |
| |
 |  |  |  | |
| | | | Search Tocris compounds for NSG1 |
| |  |  |  |  | | | | |
 |
 |
 |
 | | | | | | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for NSG1 |
|  |  |  | | | | Search ThermoFisher Antibodies for NSG1 |
| | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat NSG1 |
|
|
|
|
