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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

NSA2 Gene

protein-coding   GIFtS: 51
GCID: GC05P074062

NSA2 ribosome biogenesis homolog (S. cerevisiae)

 Explore 4 diseases affiliated with
NSA2 via our new
 Human Malady Compendium 
Biological research products
for NSA2
    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section

Aliases
NSA2 Ribosome Biogenesis Homolog (S. Cerevisiae)1 2     HUSSY-291
TINP11 2 3 5     CDK1052
HCLG11 2     HCL-G12
Hairy Cell Leukemia Protein 12 3     Ribosome Biogenesis Protein NSA2 Homolog2
TGF-Beta-Inducible Nuclear Protein 12 3     TGF Beta-Inducible Nuclear Protein 12
HUSSY292 5     TGF-Beta Inducible Nuclear Protein2
FLJ943931     

External Ids:    HGNC: 307281   Entrez Gene: 104122   Ensembl: ENSG000001643467   OMIM: 6124975   UniProtKB: O954783   

Export aliases for NSA2 gene to outside databases

Previous GC identifers: GC05P074099 GC05P069268


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for NSA2:
This gene encodes a nucleolar protein involved in cell cycle regulation and proliferation. This gene was identified
based on sequence similarity to a highly conserved Saccharomyces cerevisiae gene encoding a pre-ribosomal protein,
which is involved in large ribosomal subunit biogenesis. The encoded protein is found at elevated levels in diabetic
nephropathy. Alternative splicing results in multiple transcript variants. Several related pseudogenes have been
identified. (provided by RefSeq, Nov 2012)

UniProtKB/Swiss-Prot: NSA2_HUMAN, O95478
Function: Involved in the biogenesis of the 60S ribosomal subunit. May play a part in the quality control of pre-60S
particles (By similarity)

Gene Wiki entry for NSA2 (TINP1)


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000005.9  NC_018916.1  NT_006713.15  
Regulatory elements:
   Search SABiosciences Regulatory transcription factor binding sites for NSA2
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidNSA2 promoter sequence
   Search SABiosciences Chromatin IP Primers for NSA2

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat NSA2


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 5q13.3   Ensembl cytogenetic band:  5q13.3   HGNC cytogenetic band: 5q13.3

NSA2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
NSA2 gene location

GeneLoc information about chromosome 5         GeneLoc Exon Structure

GeneLoc location for GC05P074062:  view genomic region     (about GC identifiers)

Start:
74,062,817 bp from pter      End:
74,072,737 bp from pter
Size:
9,921 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: NSA2_HUMAN, O95478 (See protein sequence)
Recommended Name: Ribosome biogenesis protein NSA2 homolog  
Size: 260 amino acids; 30065 Da
Subunit: Component of the pre-66S ribosomal particle (By similarity)
Subcellular location: Nucleus, nucleolus

Explore the universe of human proteins at neXtProt for NSA2: NX_O95478

Post-translational modifications:

  • View modification sites using PhosphoSitePlus2
  • View neXtProt modification sites for NX_O95478

  • NSA2 Protein expression data from MOPED and PaxDb:    About this image 
    Estimated protein expression log10 (pmol).

    REFSEQ proteins (2 alternative transcripts): 
    NP_001258594.1  NP_055701.1  

    ENSEMBL proteins: 
     ENSP00000296802   ENSP00000427266  

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    Uscn Proteins for NSA2

    Gene Ontology (GO): 2 cellular component terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005730nucleolus IEA--
    GO:0030529ribonucleoprotein complex IEA--


    NSA2 for ontologies           About GeneDecksing



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    (According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    NSA2 for domains           About GeneDecksing

    1 InterPro domain/family:
     IPR022309 Ribosomal_S8e/biogenesis_NSA2

    Graphical View of Domain Structure for InterPro Entry O95478

    ProtoNet protein and cluster: O95478

    1 Blocks protein family: IPB001047 Ribosomal protein S8E

    UniProtKB/Swiss-Prot: NSA2_HUMAN, O95478
    Similarity: Belongs to the ribosomal protein S8e family


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
    About This Section

    Function Summary:

         UniProtKB/Swiss-Prot: NSA2_HUMAN, O95478
    Function: Involved in the biogenesis of the 60S ribosomal subunit. May play a part in the quality control of pre-60S
    particles (By similarity)

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    hsa-miR-33a*
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    Inhib. RNA
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    4 GenomeRNAi human phenotypes for NSA2:
     Decreased TP53 protein express  G0/1 arrest  Increased G1 DNA content  Synthetic lethal with Ras 


    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
    About This Section



    Interactions:

        Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for NSA2

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    5/110 Interacting proteins for NSA2 (O954783 ENSP000002968024) via UniProtKB, MINT, STRING, and/or I2D (see all 110)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    HIST1H4AP628053I2D: score=1 
    HIST1H4BP628053I2D: score=1 
    HIST1H4CP628053I2D: score=1 
    HIST1H4DP628053I2D: score=1 
    HIST1H4EP628053I2D: score=1 
    About this table

    Gene Ontology (GO): 1 biological process term (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006364rRNA processing IEA--


    NSA2 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section
    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for NSA2
    Search CenterWatch for drugs/clinical trials and news about NSA2 

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section

    REFSEQ mRNAs for NSA2 gene (2 alternative transcripts): 
    NM_001271665.1  NM_014886.4  

    Unigene Cluster for NSA2:

    NSA2 ribosome biogenesis homolog (S. cerevisiae)
    Hs.482526  [show with all ESTs]
    Unigene Representative Sequence: NR_073403
    4 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000296802(uc003kdk.1) ENST00000514918 ENST00000513356 ENST00000515524


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    hsa-miR-33a*
    SwitchGear 3'UTR luciferase reporter plasmidNSA2 3' UTR sequence
    Inhib. RNA
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    Clone
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    Additional cDNA sequence: 

    AF077615.1 AF372458.1 AJ012409.1 AK313784.1 AY443419.1 BC005288.1 NR_073403.1 

    18 DOTS entries:

    DT.97862484  DT.100059733  DT.100059726  DT.100059176  DT.120814492  DT.120814242  DT.121156010  DT.121211263 
    DT.120814363  DT.121287831  DT.120814469  DT.95092750  DT.95173442  DT.100776785  DT.102835948  DT.121156036 
    DT.92460306  DT.95173443 

    24/320 AceView cDNA sequences (see all 320):

    CB154675 BU688104 AA113244 AL709742 CR614877 CA867484 AI141186 AA921837 
    BQ227555 CA434370 CR620162 BG673963 CF127031 AA232252 BC005288 AA309707 
    BM719740 AA379078 W80884 BE731640 W67521 AA773137 CA438109 BU186367 

    GeneLoc Exon Structure

    4 Alternative Splicing Database (ASD) splice patterns (SP) for NSA2    About this scheme

    ExUns: 1a · 1b ^ 2a · 2b ^ 3a · 3b ^ 4 ^ 5a · 5b ^ 6a · 6b ^ 7
    SP1:                                            -     -                     
    SP2:                                                                        
    SP3:                                                                        
    SP4:                                            -     -     -     -         


    ECgene alternative splicing isoforms for NSA2

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    NSA2 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS
    CGAP TAG: TCTGCAATGA

    Microarray
    RNAseq (Illumina Body Map)
    (100×FPKM)½
    SAGE (Serial Analysis of Gene Expression)

    About this image
    See NSA2 Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for NSA2

    SOURCE GeneReport for Unigene cluster: Hs.482526
        SABiosciences Custom PCR Arrays for NSA2
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    In Situ
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    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the common ancestor of eukaryotes.

    Orthologs for NSA2 gene from 10/39 species (see all 39)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Nsa21 , 5 NSA2 ribosome biogenesis homolog (S. cerevisiae)1, 5 87.95(n)1
    99.23(a)1
      13 (50.66 cM)5
    590501  NM_021552.51  NP_067527.31 
     971294275 
    chicken
    (Gallus gallus)
    Aves NSA21 NSA2 ribosome biogenesis homolog (S. cerevisiae) 83.08(n)
    96.54(a)
      427206  NM_001006579.1  NP_001006579.1 
    lizard
    (Anolis carolinensis)
    Reptilia NSA26
    --
    98(a)
    1 ↔ 1
    2(15119740-15124839)
    tropical clawed frog
    (Xenopus tropicalis)
    Amphibia Str.792 Transcribed sequence with strong similarity to protein more 82.41(n)    BX732825.1 
    zebrafish
    (Danio rerio)
    Actinopterygii wufb34b102 wufb34b10 76.28(n)   321769  BC046083.1 
    fruit fly
    (Drosophila melanogaster)
    Insecta Ip2591 Intronic Protein 259 66.92(n)
    75.29(a)
      34419  NM_058031.4  NP_477379.1 
    worm
    (Caenorhabditis elegans)
    Secernentea CELE_W09C5.11 Protein W09C5.1 68.22(n)
    70.93(a)
      173232  NM_060986.2  NP_493387.1 
    baker's yeast
    (Saccharomyces cerevisiae)
    Saccharomycetes NSA2(YER126C)4
    NSA21
    Protein constituent of 66S pre-ribosomal particles, more4
    Nsa2p1
    61.41(n)1
    62.31(a)1
      5(414179-413394)4
    8568631, 4  NP_011052.11, 4 
    thale cress
    (Arabidopsis thaliana)
    eudicotyledons AT5G063601 Ribosomal protein S8e family protein 65.77(n)
    72.31(a)
      830524  NM_120719.3  NP_196254.1 
    rice
    (Oryza sativa)
    Liliopsida Os.100942 Oryza sativa (japonica cultivar-group) cDNA clone0 more 74.62(n)    AK060100.1 


    ENSEMBL Gene Tree for NSA2 (if available)
    TreeFam Gene Tree for NSA2 (if available) 

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    Paralogs for NSA2 gene
    1 SIMAP similar gene for NSA2 using alignment to 2 protein entries:     NSA2_HUMAN (see all proteins):
    CDK105

    NSA2 for paralogs           About GeneDecksing


    5/9 Pseudogenes.org Pseudogenes for NSA2 (see all 9)
    PGOHUM00000242316 PGOHUM00000239548 PGOHUM00000246804 PGOHUM00000244395 PGOHUM00000240420


    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/207 NCBI SNPs in NSA2 are shown (see all 207    About this table
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 5 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs727646551,2
    C,--69266222(+) TCCCAT/CGATGA 3 -- int13Minor allele frequency- C:0.16CSA WA NA 240
    rs727646561,2
    C,--69266259(+) AGGAAG/AGAGGA 3 -- int13Minor allele frequency- A:0.21CSA WA NA 240
    rs731148001,2
    C,--69267577(+) AATGAT/CAGAGA 3 -- int12Minor allele frequency- C:0.10WA 120
    rs64530811,2
    C,F,A,H,--69268047(+) TTAAAG/AGGTGA 3 -- us2k120Minor allele frequency- A:0.08NS EA NA CSA WA 2646
    rs796694941,2
    C,--69268246(+) GGTAGG/TGGGTG 3 -- us2k14Minor allele frequency- T:0.15CSA WA NA EA 360
    rs768398191,2
    F,--69268643(+) TTTCTC/TTTTGT 3 -- us2k11Minor allele frequency- T:0.03NA 120
    rs755435051,2
    --69268776(+) TTGCTC/GTCTGT 3 -- us2k11Minor allele frequency- G:0.01WA 118
    rs737629491,2
    C,--69268887(+) ATCCCG/ATACAT 3 -- us2k11Minor allele frequency- A:0.50WA 2
    rs11646931,2
    F,H,--69269127(+) tgggtG/Aaatcc 3 -- us2k15Minor allele frequency- A:0.01NS EA NA 410
    rs727646571,2
    C,--69269318(+) TACAGA/GACTGT 3 -- us2k13Minor allele frequency- G:0.21CSA WA NA 240

    HapMap Linkage Disequilibrium report for NSA2 (74062817 - 74072737 bp)
    Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
          Database of Genomic Variants (DGV) variations for NSA2: --
    Human Gene Mutation Database (HGMD): NSA2

    SABiosciences Cancer Mutation PCR Assays
    QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing NSA2
    DNA2.0 Custom Variant and Variant Library Synthesis for NSA2

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    NSA2 for disorders           About GeneDecksing

    OMIM gene information: 612497    OMIM disorders: --

    4 diseases for NSA2:    About MalaCards
    hairy cell leukemia    t-cell leukemia    leukemia    malaria


    Export disorders for NSA2 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for NSA2 gene, integrated from 9 sources (see all 18):
    (articles sorted by number of sources associating them with NSA2)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Characterization of 16 novel human genes showing high similarity to yeast sequences. (PubMed id 11124703)1, 2, 3 Stanchi F.... Valle G. (2001)
    2. Molecular analysis of the human chromosome 5q13.3 region in patients with hairy cell leukemia and identification of tumor suppressor gene candidates. (PubMed id 10486207)1, 2, 3 Wu X.... Einhorn S. (1999)
    3. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
    4. Functional proteomic analysis of human nucleolus. (PubMed id 12429849)1, 2 Scherl A.... Diaz J.-J. (2002)
    5. Functional proteomics establishes the interaction of S IRT7 with chromatin remodeling complexes and expands its role in regulation of R NA polymerase I transcription. (PubMed id 22586326)1 Tsai Y.C....Cristea I.M. (2012)
    6. Elevated levels of renal and circulating Nop-7-associa ted 2 (NSA2) in rat and mouse models of diabetes, in mesangial cells in vitro an d in patients with diabetic nephropathy. (PubMed id 22095236)1 Shahni R....Malik A.N. (2012)
    7. Integrative analysis of the ubiquitin proteome isolat ed using Tandem Ubiquitin Binding Entities (TUBEs). (PubMed id 22178446)1 Lopitz-Otsoa F....Rodriguez M.S. (2011)
    8. Mass spectrometric analysis of lysine ubiquitylation r eveals promiscuity at site level. (PubMed id 21139048)1 Danielsen J.M....Nielsen M.L. (2011)
    9. Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. (PubMed id 20068231)2 Olsen J.V....Mann M. (2010)
    10. NSA2, a novel nucleolus protein regulates cell prolif eration and cell cycle. (PubMed id 19932687)1 Zhang H....Ma D. (2010)

    (in PubMed, OMIM, and NCBI Bookshelf)
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    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 10412 HGNC: 30728 AceView: TINP1 Ensembl:ENSG00000164346 euGenes: HUgn10412
    ECgene: NSA2 H-InvDB: NSA2

    (According to HUGE)
    About This Section
      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for NSA2 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section
    Patent Information for NSA2 gene:
    Search GeneIP for patents involving NSA2

    GeneCards and IP:
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