Set Analyses:
Advanced Search

Advanced Search

 
Search By
Section (entire)
for


 



NR1I2 Gene

protein-coding   GIFtS: 66
GCID: GC03P119499

Nuclear Receptor Subfamily 1, Group I, Member 2

  See related diseases
at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
About This Section

TryGeneCards Plus

Aliases
Nuclear Receptor Subfamily 1, Group I, Member 21 2     ONR12
Orphan Nuclear Receptor PXR1 2 3     PAR2
Pregnane X Receptor1 2 3     PAR12
PXR2 3 5     PAR22
SXR2 3 5     PRR2
Orphan Nuclear Receptor PAR12 3     SAR2
Steroid And Xenobiotic Receptor2 3     Nuclear Receptor Subfamily 1 Group I Member 22
PARq2 5     Pregnane X Nuclear Receptor Variant 22
BXR2     

External Ids:    HGNC: 79681   Entrez Gene: 88562   Ensembl: ENSG000001448527   OMIM: 6030655   UniProtKB: O754693   

Export aliases for NR1I2 gene to outside databases

Previous GC identifers: GC03P116138 GC03P119128 GC03P120780 GC03P120820 GC03P120982 GC03P116874


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

TryGeneCards Plus

Entrez Gene summary for NR1I2 Gene:
This gene product belongs to the nuclear receptor superfamily, members of which are transcription factors
characterized by a ligand-binding domain and a DNA-binding domain. The encoded protein is a transcriptional
regulator of the cytochrome P450 gene CYP3A4, binding to the response element of the CYP3A4 promoter as a
heterodimer with the 9-cis retinoic acid receptor RXR. It is activated by a range of compounds that induce
CYP3A4, including dexamethasone and rifampicin. Several alternatively spliced transcripts encoding different
isoforms, some of which use non-AUG (CUG) translation initiation codon, have been described for this gene.
Additional transcript variants exist, however, they have not been fully characterized. (provided by RefSeq, Jul
2008)

GeneCards Summary for NR1I2 Gene:
NR1I2 (nuclear receptor subfamily 1, group I, member 2) is a protein-coding gene. Diseases associated with NR1I2 include biliary tract disease, and intrahepatic cholestasis of pregnancy. GO annotations related to this gene include drug binding and sequence-specific DNA binding. An important paralog of this gene is VDR.

UniProtKB/Swiss-Prot: NR1I2_HUMAN, O75469
Function: Nuclear receptor that binds and is activated by variety of endogenous and xenobiotic compounds.
Transcription factor that activates the transcription of multiple genes involved in the metabolism and secretion
of potentially harmful xenobiotics, drugs and endogenous compounds. Activated by the antibiotic rifampicin and
various plant metabolites, such as hyperforin, guggulipid, colupulone, and isoflavones. Response to specific
ligands is species-specific. Activated by naturally occurring steroids, such as pregnenolone and progesterone.
Binds to a response element in the promoters of the CYP3A4 and ABCB1/MDR1 genes

summary for NR1I2 Gene:
Pregnane X receptors (PXRs) are nuclear receptors that regulate the expression of CYP3A genes, thus
generating key enzymes involved in xenobiotic metabolism. PXRs also help regulate the induction of P-gp.
PXRs are highly expressed in the liver, small intestine and colon.

Gene Wiki entry for NR1I2 (Pregnane X receptor) Gene

PharmGKB "VIP" summary for NR1I2


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
About This Section

TryGeneCards Plus
RefSeq DNA sequence:
NC_000003.12  NC_018914.2  NT_005612.17  
Regulatory elements:
   Regulatory transcription factor binding sites in the NR1I2 gene promoter:
         PPAR-gamma1   AP-1   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmids (see all 2): NR1I2 promoter sequence
   Search Chromatin IP Primers for NR1I2

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat NR1I2


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 3q12-q13.3   Ensembl cytogenetic band:  3q13.33   HGNC cytogenetic band: 3q12-q13.3

NR1I2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
NR1I2 gene location

GeneLoc information about chromosome 3         GeneLoc Exon Structure

GeneLoc location for GC03P119499:  view genomic region     (about GC identifiers)

Start:
119,499,331 bp from pter      End:
119,537,332 bp from pter
Size:
38,002 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Cloud-Clone Corp.,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Cloud-Clone Corp., Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, Cloud-Clone Corp, and/or others.)
About This Section

TryGeneCards Plus

UniProtKB/Swiss-Prot: NR1I2_HUMAN, O75469 (See protein sequence)
Recommended Name: Nuclear receptor subfamily 1 group I member 2  
Size: 434 amino acids; 49762 Da
Subunit: Heterodimer with RXR. Interacts with NCOA1
Selected PDB 3D structures from and Proteopedia for NR1I2 (see all 12):
1ILG (3D)        1ILH (3D)        1M13 (3D)        1NRL (3D)        1SKX (3D)        2O9I (3D)    
Secondary accessions: Q006P5 Q008C8 Q96AC7 Q9UJ22 Q9UJ23 Q9UJ24 Q9UJ25 Q9UJ26 Q9UJ27 Q9UNW4
Alternative splicing: 7 isoforms:  O75469-1   O75469-2   O75469-3   O75469-4   O75469-5   O75469-6   O75469-7   

Explore the universe of human proteins at neXtProt for NR1I2: NX_O75469

Explore proteomics data for NR1I2 at MOPED

Post-translational modifications: 

  • Modification sites at neXtProt
  • Modification sites at PhosphoSitePlus

  • See NR1I2 Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins (3 alternative transcripts): 
    NP_003880.3  NP_071285.1  NP_148934.1  

    ENSEMBL proteins: 
     ENSP00000377319   ENSP00000420297   ENSP00000336528  
    Reactome Protein details: O75469

    NR1I2 Human Recombinant Protein Products:

    Browse Purified and Recombinant Proteins at EMD Millipore
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    OriGene Purified Protein for NR1I2
    OriGene Protein Over-expression Lysate for NR1I2
    OriGene MassSpec for NR1I2
    OriGene Custom Protein Services for NR1I2
    GenScript Custom Purified and Recombinant Proteins Services for NR1I2
    Novus Biologicals NR1I2 Proteins
    Novus Biologicals NR1I2 Lysates
    Browse Sino Biological Recombinant Proteins
    Browse Sino Biological Cell Lysates
    Browse ProSpec Recombinant Proteins
    Browse Proteins at Cloud-Clone Corp.

    NR1I2 Antibody Products:

    EMD Millipore Mono- and Polyclonal Antibodies for the study of NR1I2
    R&D Systems Antibodies for NR1I2 (PXR/NR1I2)
    OriGene Antibodies for NR1I2
    OriGene Custom Antibody Services for NR1I2
    Novus Biologicals NR1I2 Antibodies
    Abcam antibodies for NR1I2
    Browse Antibodies at Cloud-Clone Corp.
    ThermoFisher Antibodies for NR1I2
    LSBio Antibodies in human, mouse, rat for NR1I2

    NR1I2 Assay Products:

    Browse Kits and Assays available from EMD Millipore
    OriGene Custom Assay Services for NR1I2
    Browse R&D Systems for biochemical assays
    GenScript Custom Assay Services for NR1I2
    Browse Enzo Life Sciences for kits & assays
    Browse ELISAs at Cloud-Clone Corp.
    Browse CLIAs at Cloud-Clone Corp.


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    TryGeneCards Plus
    HGNC Gene Families:
    NR: Nuclear hormone receptors

    IUPHAR Guide to PHARMACOLOGY protein family classification: Pregnane X receptor
    1I. Vitamin D receptor-like receptors

    5 InterPro protein domains:
     IPR013088 Znf_NHR/GATA
     IPR008946 Nucl_hormone_rcpt_ligand-bd
     IPR000536 Nucl_hrmn_rcpt_lig-bd_core
     IPR001628 Znf_hrmn_rcpt
     IPR001723 Str_hrmn_rcpt

    Graphical View of Domain Structure for InterPro Entry O75469

    ProtoNet protein and cluster: O75469

    2 Blocks protein domains:
    IPB000324 Vitamin D receptor signature
    IPB001723 Steroid hormone receptor signature


    UniProtKB/Swiss-Prot: NR1I2_HUMAN, O75469
    Similarity: Belongs to the nuclear hormone receptor family. NR1 subfamily
    Similarity: Contains 1 nuclear receptor DNA-binding domain


    NR1I2 for domains           About GeneDecksing


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
    About This Section

    TryGeneCards Plus

    Molecular Function:

         UniProtKB/Swiss-Prot Summary: NR1I2_HUMAN, O75469
    Function: Nuclear receptor that binds and is activated by variety of endogenous and xenobiotic compounds.
    Transcription factor that activates the transcription of multiple genes involved in the metabolism and secretion
    of potentially harmful xenobiotics, drugs and endogenous compounds. Activated by the antibiotic rifampicin and
    various plant metabolites, such as hyperforin, guggulipid, colupulone, and isoflavones. Response to specific
    ligands is species-specific. Activated by naturally occurring steroids, such as pregnenolone and progesterone.
    Binds to a response element in the promoters of the CYP3A4 and ABCB1/MDR1 genes

         Gene Ontology (GO): Selected molecular function terms (see all 9):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003700sequence-specific DNA binding transcription factor activity ----
    GO:0003707steroid hormone receptor activity IEA--
    GO:0003713transcription coactivator activity TAS9727070
    GO:0004879ligand-activated sequence-specific DNA binding RNA polymerase II transcription factor activity IDA12578355
    GO:0004887thyroid hormone receptor activity ----
         
    NR1I2 for ontologies           About GeneDecksing


    Phenotypes:
         3 GenomeRNAi human phenotypes for NR1I2:
     Increased G1 DNA content  Synthetic lethal with Ras  Upregulation of Wnt/beta-caten 

         7 MGI mutant phenotypes (inferred from 6 alleles(MGI details for Nr1i2):
     behavior/neurological  cellular  homeostasis/metabolism  liver/biliary system  mortality/aging 
     no phenotypic analysis  reproductive system 

    NR1I2 for phenotypes           About GeneDecksing

    Animal Models:
         MGI mouse knock-outs for NR1I2: Nr1i2tm1Sakl Nr1i2tm3Arte Nr1i2tm1Rev Nr1i2tm1.2Ics

       inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for NR1I2
       inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for NR1I2

       genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for NR1I2
       genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for NR1I2

    miRNA
    Products:
        
    miRTarBase miRNAs that target NR1I2:
    hsa-let-7a-5p (MIRT002079), hsa-mir-16-5p (MIRT051226), hsa-mir-148a-3p (MIRT003998), hsa-mir-615-3p (MIRT040092)

    Block miRNA regulation of human, mouse, rat NR1I2 using miScript Target Protectors
    4 qRT-PCR Assays for microRNAs that regulate NR1I2:
    hsa-miR-140-3p hsa-miR-647 hsa-miR-3918 hsa-miR-604
    SwitchGear 3'UTR luciferase reporter plasmidNR1I2 3' UTR sequence
    Inhib. RNA
    Products:
        
    OriGene RNAi products in human, mouse, rat for NR1I2
    Predesigned siRNA for gene silencing in human, mouse, rat NR1I2

    Gene Editing
    Products:
    DNA2.0 Custom Protein Engineering Service for NR1I2

    Clone
    Products:
         
    OriGene clones in human, mouse for NR1I2 (see all 18)
    OriGene ORF clones in mouse, rat for NR1I2
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector (see all 3): NR1I2 (NM_003889)
    Browse Sino Biological Human cDNA Clones
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for NR1I2
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat NR1I2

    Cell Line
    Products:
         
    GenScript Custom overexpressing Cell Line Services for NR1I2
    Browse ESI BIO Cell Lines and PureStem Progenitors for NR1I2 
    In Situ Assay
    Products:
       

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for NR1I2


    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
    About This Section

    TryGeneCards Plus

    Subcellular locations from UniProtKB/Swiss-Prot
    NR1I2_HUMAN, O75469: Nucleus
    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    nucleus5
    cytosol2
    extracellular2
    peroxisome2
    cytoskeleton1

    Gene Ontology (GO): 2 cellular component terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634nucleus ----
    GO:0005654nucleoplasm TAS--

    NR1I2 for ontologies           About GeneDecksing


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
    About This Section

    TryGeneCards Plus

    SuperPaths for NR1I2 About   (see all 10)  
    See pathways by source

    SuperPathContained pathways About
    1Nuclear Receptor transcription pathway
    Nuclear Receptor transcription pathway0.67
    Nuclear Receptors0.67
    2Gene Expression
    Generic Transcription Pathway0.40
    Gene Expression0.40
    3Irinotecan Pathway, Pharmacokinetics
    Taxane Pathway, Pharmacokinetics0.30
    4Selected targets of HNF1
    Selected targets of HNF1
    5Selected targets of GCR alpha
    Selected targets of GCR alpha

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways


    3 BioSystems Pathways for NR1I2
        Nuclear Receptors
    Nuclear receptors in lipid metabolism and toxicity
    Drug Induction of Bile Acid Pathway

    1 Reactome Pathway for NR1I2
        Nuclear Receptor transcription pathway

    4 PharmGKB Pathways for NR1I2
        Carbamazepine Pathway, Pharmacokinetics
    Etoposide Pathway, Pharmacokinetics/Pharmacodynamics
    Metformin Pathway, Pharmacodynamic
    Taxane Pathway, Pharmacokinetics


    NR1I2 for pathways           About GeneDecksing

        Pathway & Disease-focused RT2 Profiler PCR Arrays including NR1I2: 
              Neurotrophins & Receptors in human mouse rat
              Nuclear Receptors & Coregulators in human mouse rat

    Interactions:

        GeneGlobe Interaction Network for NR1I2

    STRING Interaction Network Preview (showing 5 interactants - click image to see 20)

    Selected Interacting proteins for NR1I2 (O754691, 3 ENSP000003365284) via UniProtKB, MINT, STRING, and/or I2D (see all 31)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    RXRBP287023, ENSP000003638124I2D: score=2 STRING: ENSP00000363812
    ENSG00000206289P287023I2D: score=2 
    ENSG00000227322P287023I2D: score=2 
    ENSG00000228333P287023I2D: score=2 
    ENSG00000231321P287023I2D: score=2 
    About this table

    Gene Ontology (GO): Selected biological process terms (see all 15):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006351transcription, DNA-templated ----
    GO:0006355regulation of transcription, DNA-templated ----
    GO:0006367transcription initiation from RNA polymerase II promoter TAS--
    GO:0006805xenobiotic metabolic process IDA12578355
    GO:0007165signal transduction TAS9784494

    NR1I2 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
    About This Section

    TryGeneCards Plus
    Browse Small Molecules at EMD Millipore
       Enzo Life Sciences drugs & compounds for NR1I2
      Browse compounds at ApexBio 

    Compounds for NR1I2 available from Tocris Bioscience    About this table
    CompoundAction CAS #
    RifampicinPregnane X receptor agonist; antibiotic[13292-46-1]
    SR 12813Pregnane X receptor agonist[126411-39-0]
    Meclizine dihydrochlorideHuman pregnane X receptor agonist; H1 antagonist[1104-22-9]

    1 HMDB Compound for NR1I2    About this table
    CompoundSynonyms CAS #PubMed Ids
    Estradiol(+)-3,17b-Estradiol (see all 83)50-28-2--

    Selected DrugBank Compounds for NR1I2 (see all 11)    About this table
    CompoundSynonyms CAS #TypeActionsPubMed Ids
    ErlotinibCP358774 (see all 2)183321-74-6targetagonist18839173 19733976
    HyperforinHiperforina (see all 2)11079-53-1target--17139284 17016423
    RifampinRFP (see all 3)13292-46-1targetagonist16480505 19460945
    RifaximinRifamixin (see all 8)80621-81-4targetagonist17442842 20627999
    SR12813-- 126411-39-0target--17139284 17016423
    Vitamin Ealpha-Tocopherol (see all 2)59-02-9target--16603143 16880233
    Estradiol17beta oestradiol (see all 7)50-28-2target--17327420
    Ethinyl Estradiol17 alpha-Ethinylestradiol (see all 17)57-63-6targetagonist17327420
    N-(2,2,2-TRIFLUOROETHYL)-N-{4-[2,2,2-TRIFLUORO-1-HYDROXY-1-(TRIFLUOROMETHYL)ETHYL]PHENYL}BENZENESULFONAMIDE-- --target--10592235
    Paclitaxel7-epi-Paclitaxel (see all 8)33069-62-4targetinducer18839173

    Selected IUPHAR Ligands for NR1I2 (Pregnane X receptor) (see all 17)    About this table
    LigandTypeActionAffinityPubmed IDs
    hyperforin
    AgonistAgonist7.610852961 10974665
    SR12813
    AgonistAgonist6.710628745
    (+)-S20
    AgonistAgonist6.415872116
    pregnanedione
    AgonistAgonist6.410628745
    lovastatin
    AgonistFull agonist5.3 - 69727070
    rifampicin
    AgonistAgonist5.5 - 69784494 9727070
    schisandrin A
    AgonistAgonist5.716267138
    S20 (racemic mixture)
    AgonistAgonist5.715872116
    taxol
    AgonistAgonist5.311329060
    5beta-cholestane-3alpha,7alpha,12alpha-triol
    AgonistAgonist5.312509506

    Selected Novoseek inferred chemical compound relationships for NR1I2 gene (see all 121)    About this table
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    androstane 97.9 346 11518807 (4), 16399345 (2), 16513849 (2), 19300246 (2) (see all 99)
    citco 95 8 19588937 (2), 15933212 (1), 16857725 (1), 12611900 (1)
    sr12813 93.2 10 12909012 (4), 17974979 (1), 17573484 (1), 18096694 (1) (see all 6)
    rifampicin 88.9 251 15331348 (8), 17687072 (5), 16455805 (4), 14722235 (4) (see all 99)
    pregnenolone 16alpha-carbonitrile 88.2 70 15367577 (3), 19147857 (2), 12695359 (2), 18037906 (1) (see all 26)
    androstanol 85.2 3 11457660 (1), 10748001 (1)
    pregnane 83.8 26 15792627 (1), 17118915 (1), 17392718 (1), 18363644 (1) (see all 14)
    dmes 82.7 18 19034627 (2), 16918318 (1), 18537574 (1), 19695866 (1) (see all 7)
    hyperforin 79.5 27 12578355 (3), 14600250 (2), 19147857 (2), 20079722 (2) (see all 12)
    lithocholic acid 76.6 32 17696253 (5), 11248085 (3), 18645036 (2), 17491508 (2) (see all 12)

    1 PharmGKB related drug/compound annotation for NR1I2 gene    About this table
    Drug/compound PharmGKB Annotation
    rifampinCA  



    NR1I2 for compounds           About GeneDecksing



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, and/or QIAGEN )
    About This Section

    TryGeneCards Plus

    REFSEQ mRNAs for NR1I2 gene (3 alternative transcripts): 
    NM_003889.3  NM_022002.2  NM_033013.2  

    Unigene Cluster for NR1I2:

    Nuclear receptor subfamily 1, group I, member 2
    Hs.7303  [show with all ESTs]
    Unigene Representative Sequence: AJ009936
    5 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000393716(uc003edj.3) ENST00000466380(uc003edi.3) ENST00000474090
    ENST00000337940(uc003edk.3) ENST00000493757(uc003edl.3)
    miRNA
    Products:
         
    Block miRNA regulation of human, mouse, rat NR1I2 using miScript Target Protectors
    4 qRT-PCR Assays for microRNAs that regulate NR1I2:
    hsa-miR-140-3p hsa-miR-647 hsa-miR-3918 hsa-miR-604
    SwitchGear 3'UTR luciferase reporter plasmidNR1I2 3' UTR sequence
    Inhib. RNA
    Products:
         
    OriGene RNAi products in human, mouse, rat for NR1I2
    Predesigned siRNA for gene silencing in human, mouse, rat NR1I2
    Clone
    Products:
         
    OriGene clones in human, mouse for NR1I2 (see all 18)
    OriGene ORF clones in mouse, rat for NR1I2
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector (see all 3): NR1I2 (NM_003889)
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for NR1I2
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat NR1I2
    Primer
    Products:
        
    OriGene qPCR primer pairs and template standards for NR1I2
    OriGene qSTAR qPCR primer pairs in human, mouse for NR1I2
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat NR1I2
      QuantiTect SYBR Green Assays in human, mouse, rat NR1I2
      QuantiFast Probe-based Assays in human, mouse, rat NR1I2

    Additional mRNA sequence: 

    AB307701.1 AF061056.1 AF084644.1 AF084645.1 AJ009936.1 AJ009937.1 AK122990.1 AK223456.1 
    AK310789.1 AY091855.1 BC017304.2 HQ692837.1 HQ709177.1 

    7 DOTS entries:

    DT.205887  DT.91756601  DT.100746671  DT.101962629  DT.100746670  DT.400888  DT.95149227 

    Selected AceView cDNA sequences (see all 32):

    AI248626 AA699679 CB129483 AJ009937 AK122990 AF061056 BX089366 AJ009936 
    BM786179 NM_022002 BM850781 AF084645 BV200231 NM_033013 AF084644 BC017304 
    BV200229 CB145174 BV200230 NM_003889 AY091855 AI024672 BI252289 AW511148 

    GeneLoc Exon Structure

    4 Alternative Splicing Database (ASD) splice patterns (SP) for NR1I2    About this scheme

    ExUns: 1 ^ 2 ^ 3 ^ 4 ^ 5a · 5b ^ 6a · 6b ^ 7 ^ 8 ^ 9 ^ 10a · 10b
    SP1:        -                 -                                                   
    SP2:                          -                                                   
    SP3:                          -           -                                       
    SP4:                                                                              


    ECgene alternative splicing isoforms for NR1I2

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    TryGeneCards Plus

    NR1I2 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: CACCTTATAT
    NR1I2 Expression
    About this image


    NR1I2 expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database
     
     Liver (Hepatobiliary System)
             Hepatocytes Liver Lobule
    NR1I2 Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    NR1I2 Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.7303

    UniProtKB/Swiss-Prot: NR1I2_HUMAN, O75469
    Tissue specificity: Expressed in liver, colon and small intestine

        Pathway & Disease-focused RT2 Profiler PCR Arrays including NR1I2: 
              Neurotrophins & Receptors in human mouse rat
              Nuclear Receptors & Coregulators in human mouse rat

    Primer
    Products:
    OriGene qPCR primer pairs and template standards for NR1I2
    OriGene qSTAR qPCR primer pairs in human, mouse for NR1I2
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat NR1I2
    QuantiTect SYBR Green Assays in human, mouse, rat NR1I2
    QuantiFast Probe-based Assays in human, mouse, rat NR1I2
    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for NR1I2

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    TryGeneCards Plus

    This gene was present in the common ancestor of animals.

    Orthologs for NR1I2 gene from Selected species (see all 15)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Nr1i21 , 5 nuclear receptor subfamily 1, group I, member 21, 5 82.36(n)1
    77.39(a)1
      16 (26.64 cM)5
    181711  NM_010936.31  NP_035066.11 
     382483235 
    chicken
    (Gallus gallus)
    Aves --
    Uncharacterized protein
    20(a)
    many ↔ many
    26(3886166-3893213)
    lizard
    (Anolis carolinensis)
    Reptilia --
    Uncharacterized protein
    21(a)
    many ↔ many
    4(130492825-130518274)
    African clawed frog
    (Xenopus laevis)
    Amphibia MGC684992 hypothetical protein MGC68499 75.12(n)    AF305201.1 
    zebrafish
    (Danio rerio)
    Actinopterygii nr1i22 nuclear receptor subfamily 1, group I, member 2 78.03(n)   373875  AF502918.1 
    fruit fly
    (Drosophila melanogaster)
    Insecta Hr961 Hormone receptor-like in 96 48.69(n)
    36.68(a)
      42993  NM_079769.3  NP_524493.1 


    ENSEMBL Gene Tree for NR1I2 (if available)
    TreeFam Gene Tree for NR1I2 (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section

    TryGeneCards Plus
    Paralogs for NR1I2 gene
    VDR2  PPARD2  RARG2  PPARA2  THRB2  RARA2  NR1H42  NR1D22  
    RARB2  RORA2  RORB2  RORC2  NR1D12  NR1I32  PPARG2  NR1H22  
    THRA2  NR1H32  
    18 SIMAP similar genes for NR1I2 using alignment to 6 protein entries:     NR1I2_HUMAN (see all proteins):
    NR1i2    NR3C2    VDR    RORA    THRB    HNF4G
    ESR1    NR1I1    NR1i1    HNF4alpha    NR5A1    NR2C2
    NR1I3    NR1H3    NR1H2    NR1H4    NR1A1    THRA

    NR1I2 for paralogs           About GeneDecksing



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
    About This Section

    TryGeneCards Plus

    Selected SNPs for NR1I2 (see all 989)    About this table    
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 3 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1136413631,2
    C--116882218(+) AGGATG/TATTAT 3 -- int10--------
    rs1392947041,2
    C--116882589(+) AAAAA-/ATTATTAT 3 -- int10--------
    rs349197011,2
    C--116882602(+) TATTATTA/-CTTTA 3 -- int11Minor allele frequency- -:0.50NA 2
    rs1378712941,2
    C--119464501(+) CCTCTA/CAAACA 2 -- us2k10--------
    rs1889784861,2
    --119464640(+) TACAAA/GTTTAA 2 -- us2k10--------
    rs98595681,2
    C,F,A,H--119464770(+) AAGGTG/CGTCCT 2 -- us2k17Minor allele frequency- C:0.03NS EA NA WA 538
    rs9400871,2
    C,F,A,H--119464948(-) ATATGA/GTATCT 2 -- us2k121Minor allele frequency- G:0.06MN NS EA NA WA CSA 1740
    rs1434004561,2
    --119465030(+) ACCACA/CAGTGT 2 -- us2k10--------
    rs43346741,2
    H--119465146(+) TGTCAC/GTGCTT 2 -- us2k14Minor allele frequency- G:0.00NS EA 420
    rs9543571,2
    C,H--119465216(+) GGTAAC/TTTCTA 2 -- us2k17Minor allele frequency- T:0.00MN NS EA NA 602

    HapMap Linkage Disequilibrium report for NR1I2 (119499331 - 119537332 bp)

    Structural Variations
         Database of Genomic Variants (DGV) 1 variation for NR1I2:    About this table    
    Variant IDTypeSubtypePubMed ID
    nsv482988CNV Complex15286789

    Human Gene Mutation Database (HGMD): NR1I2
    Site Specific Mutation Identification with PCR Assays
    SeqTarget long-range PCR primers for resequencing NR1I2
    DNA2.0 Custom Variant and Variant Library Synthesis for NR1I2

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    TryGeneCards Plus
    OMIM gene information: 603065    OMIM disorders: --

    Selected diseases for NR1I2 (see all 55):    
    About MalaCards
    biliary tract disease    intrahepatic cholestasis of pregnancy    cholangitis    acute porphyria
    osteomalacia    xanthomatosis    intrahepatic cholestasis    cholestasis
    barrett esophagus/esophageal adenocarcinoma    androgen insensitivity syndrome    hepatopulmonary syndrome    porphyria
    sclerosing cholangitis    colon adenocarcinoma    leukopenia    primary sclerosing cholangitis
    biliary atresia    liver disease    rickets    inflammatory bowel disease

    1 disease from the University of Copenhagen DISEASES database for NR1I2:
    Cholestasis

    NR1I2 for disorders           About GeneDecksing

    Selected Novoseek inferred disease relationships for NR1I2 gene (see all 29)    About this table

    Disease   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    cholestasis 58.5 13 16565602 (2), 11893771 (1), 19300246 (1), 16025508 (1) (see all 8)
    xanthomatosis cerebrotendinous 49.7 3 12509506 (1), 12569201 (1)
    cholestasis intrahepatic 40.3 9 19958310 (3), 18800312 (2), 16550034 (1)
    colon adenocarcinoma 35.3 2 18332086 (1), 12788075 (1)
    inflammatory bowel diseases 33.6 9 19018724 (2), 16472590 (2), 19516190 (1), 17828778 (1) (see all 6)
    liver diseases 30.8 20 19925451 (3), 20416375 (2), 16565602 (1), 11248085 (1) (see all 13)
    osteomalacia 30.5 4 16691293 (2), 15985196 (1), 16207822 (1)
    liver damage 28.6 3 11893771 (1), 11248085 (1)
    colon carcinoma 26.5 3 18299335 (1), 12788075 (1)
    inflammation 24 24 18334180 (2), 17266551 (1), 18159929 (1), 19505191 (1) (see all 16)

    Genetic Association Database (GAD): NR1I2
    Human Genome Epidemiology (HuGE) Navigator: NR1I2 (64 documents)

    Export disorders for NR1I2 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    TryGeneCards Plus

    PubMed articles for NR1I2 gene, integrated from 10 sources (see all 789):
    (articles sorted by number of sources associating them with NR1I2)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. The human orphan nuclear receptor PXR is activated by compounds that regulate CYP3A4 gene expression and cause drug interactions. (PubMed id 9727070)1, 2, 3, 9 Lehmann J.M....Kliewer S.A. (J. Clin. Invest. 1998)
    2. Identification of a human nuclear receptor defines a new signaling pathway for CYP3A induction. (PubMed id 9770465)1, 2, 3 Bertilsson G....Berkenstam A. (Proc. Natl. Acad. Sci. U.S.A. 1998)
    3. Eleven novel single nucleotide polymorphisms in the NR1I2 (PXR) gene, four of which induce non-synonymous amino acid alterations. (PubMed id 15618712)1, 2, 9 Koyano S.... Sawada J. (Drug Metab. Pharmacokinet. 2002)
    4. A role for the pregnane X receptor in flucloxacillin-induced liver injury. (PubMed id 20222094)1, 4, 9 Andrews E....Daly A.K. (Hepatology 2010)
    5. PXR pharmacogenetics: association of haplotypes with hepatic CYP3A4 and ABCB1 messenger RNA expression and doxorubicin clearance in Asian breast cancer patients. (PubMed id 18981011)1, 4, 9 Sandanaraj E....Chowbay B. (Clin. Cancer Res. 2008)
    6. Identification of NR1I2 genetic variation using resequencing. (PubMed id 17404718)1, 4, 9 King C.R....Marsh S. (Eur. J. Clin. Pharmacol. 2007)
    7. Coactivator binding promotes the specific interaction between ligand and the pregnane X receptor. (PubMed id 12909012)1, 2, 9 Watkins R.E.... Redinbo M.R. (J. Mol. Biol. 2003)
    8. Genetic predictors of interindividual variability in hepatic CYP3A4 expression. (PubMed id 19934400)1, 4, 9 Lamba V....Schuetz E.G. (J. Pharmacol. Exp. Ther. 2010)
    9. Human CYP3A4 and murine Cyp3A11 are regulated by equol and genistein via the pregnane X receptor in a species-specific manner. (PubMed id 19297428)1, 2, 9 Li Y.... Ricketts M.L. (J. Nutr. 2009)
    10. The pregnane X receptor locus is associated with susceptibility to inflammatory bowel disease. (PubMed id 16472590)1, 4, 9 Dring M.M....McManus R. (Gastroenterology 2006)

    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

    TryGeneCards Plus
     ANDOR
    Aliases
    Disorders
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section

    TryGeneCards Plus
    Entrez Gene: 8856 HGNC: 7968 AceView: NR1I2 Ensembl:ENSG00000144852 euGenes: HUgn8856
    ECgene: NR1I2 H-InvDB: NR1I2

    (According to HUGE)
    About This Section

    TryGeneCards Plus
      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
    About This Section

    TryGeneCards Plus
    NameDescription
    PharmGKB entry for NR1I2 Pharmacogenomics, SNPs, Pathways
    SeattleSNPshttp://pga.gs.washington.edu/data/nr1i2/

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section

    TryGeneCards Plus
    Patent Information for NR1I2 gene:
    Search GeneIP for patents involving NR1I2

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript, and ESI BIO, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
    Advanced Cell Diagnostics, Animal models from inGenious Targeting Laboratory, genOway)
    About This Section

    TryGeneCards Plus

     EMD Millipore Mono- and Polyclonal Antibodies for the study of NR1I2
     Browse Purified and Recombinant Proteins at EMD Millipore
     Browse Small Molecules at EMD Millipore
     Browse Kits and Assays available from EMD Millipore
     
     EMD Millipore genomic analysis products

      
     Antibodies for NR1I2 (PXR/NR1I2)   Browse Cell Culture Products  
     Browse ELISAs   Browse Flow Cytometry Kits  
     Browse Primer Pairs   Browse Enzyme Activity Assays/Reagents  
     Browse ELISpot/FluoroSpot Kits/Development Modules   Browse TFB/Immunoprecipitation Assays  
     Browse Apoptosis Detection Kits/Reagents   Browse Ubiquitin Proteasome Pathway (UPP) Assay Kits/Reagents  
     Browse DNA Damage/Repair Kits/Reagents   Browse Luminex Assays  
     Browse Cell Selection/Detection Kits/Reagents   Browse Secondary Antibodies/Controls/Staining Reagents  
     Browse Recombinant/Natural Proteins   Browse Stem Cell Products  
     Browse cDNA Clones   Browse Proteome Profiler Antibody Arrays  
     OriGene Antibodies for NR1I2   OriGene RNAi products in human, mouse, rat for NR1I2  
     OriGene qPCR primer pairs and template standards for NR1I2   OriGene Protein Over-expression Lysate for NR1I2  
     OriGene MassSpec something-or-other for NR1I2   OriGene clones in human, mouse for NR1I2  
     OriGene qSTAR qPCR primer pairs in human, mouse for NR1I2   OriGene Purified Protein for NR1I2  
     OriGene ORF clones in mouse, rat for NR1I2   OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling  
     OriGene Custom Antibody Services for NR1I2   OriGene Custom Protein Services for NR1I2  

     
     
     Block miRNA regulation of human, mouse, rat NR1I2 using miScript Target Protectors SeqTarget long-range PCR primers for resequencing NR1I2
     DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat NR1I2 Predesigned siRNA for gene silencing in human, mouse, rat NR1I2
     QuantiFast Probe-based Assays in human, mouse, rat NR1I2 QuantiTect SYBR Green Assays in human, mouse, rat NR1I2
     PCR Arrays including human, mouse, rat NR1I2 Search Chromatin IP Primers for NR1I2
     Pre-validated RT2 qPCR Primer Assay in human, mouse, rat NR1I2  GeneGlobe Interaction Network for NR1I2
     Regulatory tfbs in NR1I2 promoter
     GenScript Custom Purified and Recombinant Proteins Services for NR1I2 GenScript cDNA clones with any tag delivered in your preferred vector for NR1I2
     GenScript Custom Assay Services for NR1I2 GenScript Custom overexpressing Cell Line Services for NR1I2
     CloneReady with Over 120,000 Genes  Gene Synthesis: Any Gene in Any Vector
     Vector-based siRNA and miRNA, Ready for Transfection Gene Mutant Library, Variants up to 10^11
     Plasmid Preparation Custom Peptide Services
     Search for Antibodies & Assays

     Tocris compounds for NR1I2
     Browse Sino Biological Proteins
     Browse Sino Biological Cell Lysates
     Browse Sino Biological cDNA Clones
     4000+ Proteins
     Search Sino Biological for antibodies, proteins & pathways
     Protein Production Services
     Transfection Reagents
     Protein A/G/L resins
     Isotyping reagents
     Drugs for NR1I2
     Search www.enzolifesciences.com for proteins, assays, substrates, inhibitors & antibodies

     Novus Tissue Slides
     NR1I2 antibodies
     NR1I2 proteins
     NR1I2 lysates
     Antibodies for NR1I2
     See all of Abcam's Antibodies, Kits and Proteins for NR1I2
     Custom Antibody / Protein Production Service
     Bulk Purchasing
     Advantages of Rabbit Monoclonal antibodies
     Abcam protocols and scientific support
     Browse ProSpec Recombinant Proteins
     Browse Proteins at Cloud-Clone Corp.
     Browse Antibodies at Cloud-Clone Corp.
     Browse ELISAs at Cloud-Clone Corp.
     Browse CLIAs at Cloud-Clone Corp.



     Browse ESI BIO Cell Lines and PureStem Progenitors for NR1I2
     Gene Synthesis
     Protein Engineering
     Variant Library Synthesis
     Codon Optimization
     Protein Production and Purification
     Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for NR1I2
     SwitchGear 3'UTR luciferase reporter plasmids for NR1I2
     SwitchGear Promoter luciferase reporter plasmids for NR1I2
     ThermoFisher Antibodies for NR1I2
     Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat NR1I2
     inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for NR1I2
     inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for NR1I2
     LSBio Antibodies in human, mouse, rat for NR1I2
    Customized transgenic rodents for:
     Humanization
     Biomarker expression
     Off-target effect monitoring
     Translational medicine
     Tissue-specific gene expresssion
     Time-controlled gene expresssion
     Browse compounds at ApexBio
           
    GeneCards Homepage - Last full update: 7 May 2014 - Incrementals: 9 May 2014 , 2 Jun 2014 , 26 Jun 2014 , 30 Jun 2014

    View Random Gene

    Category
    (GIFtS: )
    GIFtS Group
    The GeneCards human gene database gene index: 1 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 


    Developed at the Crown Human Genome Center, Department of Molecular Genetics, the Weizmann Institute of Science

    Hot genes      Disease genes      NR1I2 gene at Home site.
    Version: 3.12.166 28 Aug 2014
    hostname: 356977-web1.xennexinc.com index build: 126 solr: 1.4