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Aliases for NPFFR2 Gene

Aliases for NPFFR2 Gene

  • Neuropeptide FF Receptor 2 2 3
  • GPR74 3 4 6
  • NPFF2 3 4 6
  • NPGPR 3 4 6
  • Neuropeptide G-Protein Coupled Receptor 3 4
  • G-Protein Coupled Receptor HLWAR77 3 4
  • G Protein-Coupled Receptor 74 2 3
  • G-Protein Coupled Receptor 74 3 4
  • Neuropeptide FF 2 2 3
  • HLWAR77 3

External Ids for NPFFR2 Gene

Previous Symbols for NPFFR2 Gene

  • GPR74

Summaries for NPFFR2 Gene

Entrez Gene Summary for NPFFR2 Gene

  • This gene encodes a member of a subfamily of G-protein-coupled neuropeptide receptors. This protein is activated by the neuropeptides A-18-amide (NPAF) and F-8-amide (NPFF) and may function in pain modulation and regulation of the opioid system. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2009]

GeneCards Summary for NPFFR2 Gene

NPFFR2 (Neuropeptide FF Receptor 2) is a Protein Coding gene. Among its related pathways are Signaling by GPCR and Signaling by GPCR. GO annotations related to this gene include G-protein coupled receptor activity and opioid receptor binding. An important paralog of this gene is NPFFR1.

UniProtKB/Swiss-Prot for NPFFR2 Gene

  • Receptor for NPAF (A-18-F-amide) and NPFF (F-8-F-amide) neuropeptides, also known as morphine-modulating peptides. Can also be activated by a variety of naturally occurring or synthetic FMRF-amide like ligands. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system

Tocris Summary for NPFFR2 Gene

  • Neuropeptide FF/AF (NPFF) receptors are a group of Gi/Go-protein coupled receptors that are divided into two subtypes; NPFF1 and NPFF2. NPFF receptors act through inhibition of adenylyl cyclase and activation of phospholipase C and are coupled to voltage-gated N-type calcium channels. NPFF receptors have been implicated in hormonal modulation, regulation of food intake, thermoregulation and nociception through modulation of the opioid system. NPFF receptors are distributed across the central nervous system, but each subtype has specific localizations. NPFF1 are found in their highest concentrations in the septum, nucleus of the tractus solitarius and hypothalamus, suggesting their participation in neuroendocrine functions. NPFF2 are found in their highest concentrations in the diencephalon and superficial layers of the spinal cord, suggesting their participation in the modulation of pain. The human genes encoding NPFF1 and NPFF2 receptors are located on chromosomes 4q21 and 10q21-22 respectively.

Gene Wiki entry for NPFFR2 Gene

No data available for PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for NPFFR2 Gene

Genomics for NPFFR2 Gene

Genomic Location for NPFFR2 Gene

72,031,804 bp from pter
72,148,201 bp from pter
116,398 bases
Plus strand

Genomic View for NPFFR2 Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for NPFFR2 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for NPFFR2 Gene

Regulatory Elements for NPFFR2 Gene

Proteins for NPFFR2 Gene

  • Protein details for NPFFR2 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Recommended name:
    Neuropeptide FF receptor 2
    Protein Accession:
    Secondary Accessions:
    • Q96RV1
    • Q9NR49

    Protein attributes for NPFFR2 Gene

    522 amino acids
    Molecular mass:
    60270 Da
    Quaternary structure:
    No Data Available
    • Sequence=AAK58513.1; Type=Frameshift; Positions=503; Evidence={ECO:0000305};

    Alternative splice isoforms for NPFFR2 Gene


neXtProt entry for NPFFR2 Gene

Proteomics data for NPFFR2 Gene at MOPED

Post-translational modifications for NPFFR2 Gene

  • Glycosylation at Asn110, Asn122, Asn133, and Asn300

Other Protein References for NPFFR2 Gene

ENSEMBL proteins:
Reactome Protein details:
REFSEQ proteins:

No data available for DME Specific Peptides for NPFFR2 Gene

Domains for NPFFR2 Gene

Gene Families for NPFFR2 Gene

  • RFAPR :GPCR / Class A : RF amide peptide receptors
  • NPFFR :GPCR / Class A : Neuropeptide receptors : FF/AF

Graphical View of Domain Structure for InterPro Entry



  • Belongs to the G-protein coupled receptor 1 family.:
    • Q9Y5X5
genes like me logo Genes that share domains with NPFFR2: view

Function for NPFFR2 Gene

Molecular function for NPFFR2 Gene

UniProtKB/Swiss-Prot Function: Receptor for NPAF (A-18-F-amide) and NPFF (F-8-F-amide) neuropeptides, also known as morphine-modulating peptides. Can also be activated by a variety of naturally occurring or synthetic FMRF-amide like ligands. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system

Gene Ontology (GO) - Molecular Function for NPFFR2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004930 G-protein coupled receptor activity TAS 10851242
GO:0008188 neuropeptide receptor activity IBA --
GO:0031628 opioid receptor binding IEA --
GO:0042277 peptide binding IBA --
genes like me logo Genes that share ontologies with NPFFR2: view

Phenotypes for NPFFR2 Gene

genes like me logo Genes that share phenotypes with NPFFR2: view

No data available for Enzyme Numbers (IUBMB) , Animal Models , miRNA , Transcription Factor Targeting and HOMER Transcription for NPFFR2 Gene

Localization for NPFFR2 Gene

Subcellular locations from UniProtKB/Swiss-Prot for NPFFR2 Gene

Cell membrane; Multi-pass membrane protein.

Subcellular locations from

Jensen Localization Image for NPFFR2 Gene COMPARTMENTS Subcellular localization image for NPFFR2 gene
Compartment Confidence
plasma membrane 5
cytoskeleton 4

Gene Ontology (GO) - Cellular Components for NPFFR2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005886 plasma membrane TAS --
GO:0005887 integral component of plasma membrane TAS 10079187
GO:0015629 actin cytoskeleton IDA --
GO:0016021 integral component of membrane --
genes like me logo Genes that share ontologies with NPFFR2: view

Pathways for NPFFR2 Gene

genes like me logo Genes that share pathways with NPFFR2: view

Pathways by source for NPFFR2 Gene

Gene Ontology (GO) - Biological Process for NPFFR2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0007186 G-protein coupled receptor signaling pathway TAS 10079187
GO:0007218 neuropeptide signaling pathway IBA --
GO:0007268 synaptic transmission IBA --
GO:0009582 detection of abiotic stimulus TAS 10851242
GO:0032870 cellular response to hormone stimulus IBA --
genes like me logo Genes that share ontologies with NPFFR2: view

Compounds for NPFFR2 Gene

(3) Tocris Compounds for NPFFR2 Gene

Compound Action Cas Number
Neuropeptide SF (mouse, rat) NPFF1 and NPFF2 agonist [230960-31-3]
RF 9 Selective NPFF1 and NPFF2 antagonist [876310-60-0]
RFRP 3 (human) NPFF1 agonist; GnIH homolog [311309-27-0]

(1) HMDB Compounds for NPFFR2 Gene

Compound Synonyms Cas Number PubMed IDs
  • Ca

(13) IUPHAR Ligand for NPFFR2 Gene

Ligand Type Action Affinity Pubmed IDs
dNPA Agonist Full agonist 10.6
[125I]EYF Agonist Full agonist 10.2
neuropeptide FF Agonist Full agonist 9.9
Y-RFRP-3 Agonist Full agonist 9.4
[3H]EYF Agonist Full agonist 9.3
RFRP-3 Agonist Full agonist 9.3
1DMe Agonist Full agonist 9
pancreatic polypeptide Agonist Full agonist 8
BIBP3226 Antagonist Antagonist 7.8
EFWSLAAPQRF-NH2 Agonist Full agonist 7.3
RF9 Antagonist Antagonist 7.2
AC263093 Agonist Full agonist 5.9
[125I]NPFF Agonist Full agonist

(2) Novoseek inferred chemical compound relationships for NPFFR2 Gene

Compound -log(P) Hits PubMed IDs
npff 96.1 9
bibp3226 88 1
genes like me logo Genes that share compounds with NPFFR2: view

Transcripts for NPFFR2 Gene

Unigene Clusters for NPFFR2 Gene

Neuropeptide FF receptor 2:
Representative Sequences:

Alternative Splicing Database (ASD) splice patterns (SP) for NPFFR2 Gene

ExUns: 1a · 1b ^ 2 ^ 3 ^ 4 ^ 5 ^ 6 ^ 7a · 7b · 7c
SP1: - -
SP2: - - -
SP3: -
SP4: - - - -

Relevant External Links for NPFFR2 Gene

GeneLoc Exon Structure for
ECgene alternative splicing isoforms for

Expression for NPFFR2 Gene

mRNA expression in normal human tissues for NPFFR2 Gene

mRNA differential expression in normal tissues according to GTEx for NPFFR2 Gene

This gene is overexpressed in Brain - Hypothalamus (5.5) and Brain - Anterior cingulate cortex (BA24) (4.4).

SOURCE GeneReport for Unigene cluster for NPFFR2 Gene Hs.99231

mRNA Expression by UniProt/SwissProt for NPFFR2 Gene

Tissue specificity: Isoform 1 is abundant in placenta. Relatively highly expressed in thymus, testis, and small intestine. Expressed at low levels in several tissues including spleen, prostate, brain, heart, ovary, colon, kidney, lung, liver and pancreas and not expressed in skeletal muscle and leukocytes. Isoform 2 expression is highest in placenta (but at relatively low level compared to isoform 1). Very low level of expression in numerous tissues including adipose tissue and many brain regions. Isoform 3 is expressed in brain and heart and, at lower levels, in kidney, liver, lung and pancreas.
genes like me logo Genes that share expressions with NPFFR2: view

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery and Protein expression for NPFFR2 Gene

Orthologs for NPFFR2 Gene

This gene was present in the common ancestor of animals.

Orthologs for NPFFR2 Gene

Organism Taxonomy Gene Similarity Type Details
(Pan troglodytes)
Mammalia NPFFR2 35
  • 99.23 (n)
  • 98.66 (a)
  • 99 (a)
(Bos Taurus)
Mammalia NPFFR2 35
  • 87.99 (n)
  • 86.26 (a)
  • 86 (a)
(Canis familiaris)
Mammalia NPFFR2 35
  • 86.26 (n)
  • 81.62 (a)
  • 82 (a)
(Mus musculus)
Mammalia Npffr2 35
  • 82.25 (n)
  • 79.76 (a)
Npffr2 16
Npffr2 36
  • 79 (a)
(Monodelphis domestica)
Mammalia NPFFR2 36
  • 72 (a)
(Ornithorhynchus anatinus)
Mammalia NPFFR2 36
  • 56 (a)
(Rattus norvegicus)
Mammalia Npffr2 35
  • 81.57 (n)
  • 78.37 (a)
(Gallus gallus)
Aves NPFFR2 35
  • 68.61 (n)
  • 67.4 (a)
  • 65 (a)
(Anolis carolinensis)
Reptilia NPFFR2 36
  • 61 (a)
African clawed frog
(Xenopus laevis)
Amphibia Xl.19325 35
tropical clawed frog
(Silurana tropicalis)
Amphibia npffr2 35
  • 65.54 (n)
  • 65.38 (a)
(Danio rerio)
Actinopterygii npffr2.1 35
  • 64.95 (n)
  • 65.2 (a)
NPFFR2.1 36
  • 61 (a)
npffr2.2 36
  • 55 (a)
African malaria mosquito
(Anopheles gambiae)
Insecta GPRNNA7 35
  • 46.44 (n)
  • 34 (a)
fruit fly
(Drosophila melanogaster)
Insecta SIFR 35
  • 48.15 (n)
  • 35 (a)
  • 18 (a)
Species with no ortholog for NPFFR2:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea squirt (Ciona intestinalis)
  • sea squirt (Ciona savignyi)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for NPFFR2 Gene

Gene Tree for NPFFR2 (if available)
Gene Tree for NPFFR2 (if available)

Paralogs for NPFFR2 Gene

Selected SIMAP similar genes for NPFFR2 Gene using alignment to 2 proteins:

genes like me logo Genes that share paralogs with NPFFR2: view

Variants for NPFFR2 Gene

Sequence variations from dbSNP and Humsavar for NPFFR2 Gene

SNP ID Clin Chr 04 pos Sequence Context AA Info Type MAF
rs983345 -- 72,148,081(-) AAGTT(G/T)GGAAA utr-variant-3-prime
rs993280 -- 72,145,149(-) GATGA(A/G)TAAGA intron-variant
rs2102651 -- 72,132,313(+) gttcc(A/G)tcaaa intron-variant
rs3062262 -- 72,131,973(+) GATAA(-/AAA)TTATT intron-variant
rs4129733 -- 72,097,303(+) GAAAT(G/T)TTCCA intron-variant

Structural Variations from Database of Genomic Variants (DGV) for NPFFR2 Gene

Variant ID Type Subtype PubMed ID
nsv879477 CNV Gain 21882294
nsv879480 CNV Loss 21882294
esv270098 CNV Insertion 20981092
nsv508287 CNV Loss 20534489
esv2289844 CNV Deletion 18987734
esv2663080 CNV Deletion 23128226
nsv518185 CNV Loss 19592680
esv269457 CNV Insertion 20981092
esv273932 CNV Insertion 20981092
esv22388 CNV Loss 19812545
nsv829968 CNV Gain 17160897

Relevant External Links for NPFFR2 Gene

HapMap Linkage Disequilibrium report
Human Gene Mutation Database (HGMD)

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for NPFFR2 Gene

Disorders for NPFFR2 Gene

Relevant External Links for NPFFR2

Genetic Association Database (GAD)
Human Genome Epidemiology (HuGE) Navigator
genes like me logo Genes that share disorders with NPFFR2: view

No data available for UniProtKB/Swiss-Prot for NPFFR2 Gene

Publications for NPFFR2 Gene

  1. Identification and characterization of two G protein-coupled receptors for neuropeptide FF. (PMID: 11024015) Bonini J.A. … Borowsky B. (J. Biol. Chem. 2000) 3 4 23
  2. Receptor for the pain modulatory neuropeptides FF and AF is an orphan G protein-coupled receptor. (PMID: 10851242) Elshourbagy N.A. … Sarau H.M. (J. Biol. Chem. 2000) 2 3 4
  3. Sequence and tissue distribution of a novel G-protein-coupled receptor expressed prominently in human placenta. (PMID: 10079187) Cikos S. … Koppel J. (Biochem. Biophys. Res. Commun. 1999) 2 3 4
  4. Molecular cloning and characterisation of GPR74 a novel G-protein coupled receptor closest related to the Y-receptor family. (PMID: 10837915) Parker R.M.C. … Herzog H. (Brain Res. Mol. Brain Res. 2000) 3 4
  5. A common haplotype in the G-protein-coupled receptor gene GPR74 is associated with leanness and increased lipolysis. (PMID: 17503329) Dahlman I. … Arner P. (Am. J. Hum. Genet. 2007) 3 48

Products for NPFFR2 Gene

Sources for NPFFR2 Gene

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