NPBWR2 Gene
protein-coding GIFtS: 53
GCID: GC20M062737
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neuropeptides B/W receptor 2(Previous name: G protein-coupled receptor 8 )
(Previous symbol: GPR8)
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Aliases
for NPBWR2 gene
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc,
7Ensembl,
8DME,
9miRBase,
and/or 10fRNAdb)
About This Section
|
| Aliases |
|---|
| Neuropeptides B/W Receptor 21 2 | | GPR81 2 3 5 | | G Protein-Coupled Receptor 81 2 | | G-Protein Coupled Receptor 82 3 | | Neuropeptides B/W Receptor Type 22 | | Opioid-Somatostatin-Like Receptor 82 |
Export aliases for NPBWR2 gene to outside databasesPrevious GC identifers: GC20M062209 GC20M059465 |
Summaries
for NPBWR2 gene(According to Entrez Gene,
Tocris Bioscience,
Wikipedia's
Gene Wiki,
PharmGKB,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
|
Entrez Gene summary for NPBWR2:
The protein encoded by this gene is an integral membrane protein and G protein-coupled receptor. The encoded protein issimilar in sequence to another G protein-coupled receptor (GPR7), and it is structurally similar to opioid andsomatostatin receptors. This protein binds neuropeptides B and W. This gene is intronless and is expressed primarilyin the frontal cortex of the brain. (provided by RefSeq, Jul 2008)
UniProtKB/Swiss-Prot: NPBW2_HUMAN, P48146Function: Interacts specifically with a number of opioid ligands. Receptor for neuropeptides B and W, which may beinvolved in neuroendocrine system regulation, food intake and the organization of other signals
Gene Wiki entry for NPBWR2 (Neuropeptides B/W receptor 2)
|
Genomic Views
for NPBWR2 gene
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to
UCSC (hg19) and
Ensembl (release 69),
Regulatory elements and Epigenetics data according to
QIAGEN,
SABiosciences, and/or
SwitchGear Genomics)
About This Section
| RefSeq DNA sequence:- NC_000020.10 NC_018931.1 NT_011333.6
Regulatory elements:
 SABiosciences Regulatory transcription factor binding sites in the NPBWR2 gene promoter:
GATA-3 p53 SREBP-1c E47 C/EBPalpha GATA-2 RORalpha1 PPAR-gamma1 Hand1 PPAR-gamma2
Other transcription factors
Search SABiosciences Chromatin IP Primers for NPBWR2
Epigenetics:
| | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat NPBWR2 |
Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track
Entrez Gene cytogenetic band: 20q13.3 Ensembl cytogenetic band: 20q13.33 HGNC cytogenetic band: 20q13.3NPBWR2 Gene in genomic location: bands according to Ensembl, locations according to
 (and/or Entrez Gene and/or Ensembl if different)
GeneLoc information about chromosome 20 GeneLoc Exon Structure GeneLoc location for GC20M062737: view genomic region
(about GC identifiers)
Start:
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62,737,173 bp from pter |
End:
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62,738,524 bp from pter |
Size:
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1,352 bases |
Orientation:
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minus strand |
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Proteins
for NPBWR2 gene
(According to
1UniProtKB,
HORDE,
neXtProt,
Ensembl,
and/or Reactome,
Modification sites according to 2PhosphoSitePlus,
Specific Peptides from DME,
Protein expression images according to data from SPIRE MOPED and PaxDb,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from
EMD Millipore,
R&D Systems,
GenScript,
Enzo Life Sciences,
OriGene,
Novus Biologicals,
Sino Biological,
ProSpec, and/or
Uscn,
Biochemical Assays by
EMD Millipore,
R&D Systems,
OriGene,
GenScript,
Cell Signaling Technology,
Enzo Life Sciences, and/or
Uscn,
Ontologies according to Gene
Ontology Consortium 01 Mar 2013 and
Entrez Gene,
Antibodies by
EMD Millipore,
R&D Systems,
GenScript,
Cell Signaling Technology,
OriGene,
Novus Biologicals,
Thermo Fisher Scientific,
Abcam, and/or
Uscn)
About This Section
|
UniProtKB/Swiss-Prot: NPBW2_HUMAN, P48146 (See
protein sequence)Recommended Name: Neuropeptides B/W receptor type 2 Size: 333 amino acids; 36861 Da
Subcellular location: Cell membrane; Multi-pass membrane protein
Secondary accessions: Q6NWQ6 Q9H4K3Explore the universe of human proteins at neXtProt for NPBWR2: NX_P48146
Post-translational modifications:
View modification sites using PhosphoSitePlus2View neXtProt modification sites for NX_P48146
NPBWR2 Protein expression data from MOPED and PaxDb: About this image 
REFSEQ proteins: NP_005277.2
ENSEMBL proteins: ENSP00000358783 Reactome Protein details: P48146
Human Recombinant Protein Products:
Gene Ontology (GO): 2 cellular component terms (GO ID links to tree view): About this table
NPBWR2 for ontologies About GeneDecksing
NPBWR2 Antibody Products:
Assay Products for NPBWR2: |
Protein
Domains /
Families
for NPBWR2 gene(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
|
NPBWR2 for domains About GeneDecksing
3 InterPro domains/families:Graphical View of Domain Structure for InterPro Entry P48146ProtoNet protein and cluster: P48146 UniProtKB/Swiss-Prot: NPBW2_HUMAN, P48146Similarity: Belongs to the G-protein coupled receptor 1 family |
Function
for NPBWR2 gene
(According to 1UniProtKB,
Genatlas,
LifeMap Discovery™,
IUBMB, and/or
2DME,
Human phenotypes from GenomeRNAi,
Animal models from MGI Mar 06 2013,
bound targets from SABiosciences,
miRNA Gene Targets from miRTarBase
shRNA from
OriGene,
RNAi from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
microRNA from QIAGEN,
Gene Editing from DNA2.0,
Clones from EMD Millipore,
OriGene,
SwitchGear Genomics,
GenScript,
Sino Biological,
DNA2.0,
and Vector BioLabs,
Cell Lines from GenScript,
LifeMap BioReagents,
In Situ Hybridization Assays from Advanced Cell Diagnostics,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene.)
About This Section
|
Function Summary:
UniProtKB/Swiss-Prot: NPBW2_HUMAN, P48146Function: Interacts specifically with a number of opioid ligands. Receptor for neuropeptides B and W, which may beinvolved in neuroendocrine system regulation, food intake and the organization of other signals
Clone
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OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for NPBWR2
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
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In Situ Assay
Products: |
 | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for NPBWR2 |
Gene Ontology (GO): 2 molecular function terms (GO ID links to tree view): About this table
NPBWR2 for ontologies About GeneDecksing
|
Pathways & Interactions
for NPBWR2 gene
(Pathways according to
EMD Millipore,
R&D Systems,
Cell Signaling Technology,
KEGG,
PharmGKB,
BioSystems,
Reactome,
Tocris Bioscience,
GeneGo (Thomson Reuters),
QIAGEN,
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Interaction Networks according to
SABiosciences,
and/or STRING,
Interactions according to 1UniProtKB,
2MINT,
3I2D, and/or
4STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene).
About This Section
|
Unified GeneCards pathways About this table
See pathways by source
| Super-pathway | contained gene-specific pathways |
|---|
| 1 | Class A/1 (Rhodopsin-like receptors) | | | 2 | Signaling by GPCR | | | 3 | Neuropeptides signaling through G-protein alpha-i & G-protein alpha-q | |
Pathway sources
See GeneCards unified pathways
Show all pathways
1 EMD Millipore Pathway for NPBWR2
1  BioSystems Pathway for NPBWR2
5/7 
Reactome Pathways for NPBWR2 (see all 7)
1 
Kegg Pathway (Kegg details for NPBWR2):
NPBWR2 for pathways About GeneDecksing
Interactions:
SABiosciences Gene Network CentralTM Interacting Genes and Proteins Network for NPBWR2
STRING Interaction
Network Preview (showing 5 interactants - click image to see 25)
5/143 Interacting proteins for NPBWR2 (P481463 ENSP000003587834) via UniProtKB, MINT, STRING, and/or I2D (see all 143)About this table
Gene Ontology (GO): 2 biological process terms (GO ID links to tree view): About this table
NPBWR2 for ontologies About GeneDecksing
|
Drugs & Compounds
for NPBWR2 gene(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
EMD Millipore, Tocris Bioscience
HMDB,
BitterDB, and/or
Novoseek, and Drugs according to
DrugBank,
Enzo Life Sciences, and/or
PharmGKB, with drugs/clinical trials/news
search links to CenterWatch)
About This Section
|
 Browse Tocris compounds for NPBWR2
Search CenterWatch for drugs/clinical trials and news about NPBWR2 / NPBW2
|
Transcripts
for NPBWR2 gene(Secondary structures according to
fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
transcript ids from Ensembl
with links to UCSC,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
shRNA from
OriGene,
microRNA from QIAGEN,
Tagged/untagged cDNA clones from
OriGene,
SwitchGear Genomics,
GenScript,
DNA2.0,
Vector BioLabs,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN
)
About This Section
|
REFSEQ mRNAs for NPBWR2 gene: NM_005286.2 Unigene Cluster for NPBWR2: Neuropeptides B/W receptor 2 Hs.248118 [show with all ESTs]Unigene Representative Sequence: NM_0052861 Ensembl transcript including schematic representation, and UCSC links where relevant: ENST00000369768(uc011abt.2)
Clone
Products: | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for NPBWR2
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for NPBWR2
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling
| | | GenScript: all cDNA clones in your preferred vector: NPBWR2 (NM_005286) | | | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for NPBWR2 | | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat NPBWR2 |
Additional cDNA sequence: BC067481.1 BC067482.1 BC067483.1 1 DOTS entry: DT.92021785 7 AceView cDNA sequences: BC067483 NM_005286 BC067481 BC067482 CD559647 CD559648 CD559646
GeneLoc Exon Structure
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Expression
for NPBWR2 gene
(RNA expression data according to
H-InvDB,
NONCODE,
miRBase, and
RNAdb,
Expression images according to data from
BioGPS,
Illumina Human BodyMap, and
CGAP
SAGE,
Sets of similar genes according to GeneDecks,
in vivo and in vitro expression data from LifeMap Discovery™,
plus additional links to
Genevestigator, and/or
SOURCE, and/or
BioGPS, and/or
UniProtKB,
PCR Arrays from
SABiosciences,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN,
In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section
| NPBWR2 expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: CCCTGGCGCA
About this image
See NPBWR2 Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for NPBWR2
SOURCE GeneReport for Unigene cluster: Hs.248118
UniProtKB/Swiss-Prot: NPBW2_HUMAN, P48146Tissue specificity: Detected at high levels in caudate nucleus, hyppocampus and amygdala; at moderate levels in theadult brain, thalamus, parietal cortex, pituitary gland, adrenal gland and lymph nodes
SABiosciences Expression via Pathway-Focused PCR Array including NPBWR2: | G Protein Coupled Receptors 384HT in human mouse rat |
Primer
Products: | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for NPBWR2
Browse OriGene validated miRNA SYBR primer pairs
| | | SABiosciences RT2 qPCR Primer Assay in human, mouse / rat NPBWR2 | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat NPBWR2 | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat NPBWR2 | In Situ
Assay Products: |
| Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for NPBWR2 |
Orthologs
for NPBWR2 gene
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
,
5MGI Mar 06 2013,
with possible further links to
Flybase
and/or
WormBase,
and/or
6Ensembl pan taxonomic compara ,
Gene Trees according to Ensembl and
TreeFam)
About This Section
|
This gene was present in the common ancestor of animals.
Orthologs for NPBWR2 gene from 5/12 species (see all 12) About this table
ENSEMBL Gene Tree for NPBWR2 (if available)
TreeFam Gene Tree for NPBWR2 (if available) |
Paralogs
for NPBWR2 gene(Paralogs according to
1HomoloGene,
2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
About This Section
| Paralogs for NPBWR2 gene
- SSTR32 OPRD12 SSTR42 GALR22 MCHR22 SSTR12 GALR32 OPRL12
- NPBWR12 OPRK12 KISS1R2 GALR12 SSTR52 SSTR22 OPRM12 MCHR12
18/36 SIMAP similar genes for NPBWR2 using alignment to 2 protein entries: NPBW2_HUMAN (see all proteins)
(see all similar genes):NPBWR1 OPRK1 OPRD1 SSTR1 CCR3 OPRM1
SSTR4 OPRL1 SSTR2 CCR-5 CMKLR1 SSTR3
SSTR5 CCR1 CCR5 CCR7 CCR8 GPR24
NPBWR2 for paralogs About GeneDecksing
|
Genomic Variants
for NPBWR2 gene(SNPs/Variants according to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE,
UniProtKB, and
DNA2.0,
Linkage Disequilibrium by HapMap,
Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene
Mutation Database (HGMD) and the Locus Specific Mutation
Databases (LSDB), Blood group antigen gene mutations by BGMUT,
Resequencing Primers from QIAGEN,
Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section
|
| Genomic Data | Transcription Related Data | Allele Frequencies | | SNP ID | Valid | Clinical
significance | Chr 20 pos | Sequence | # | AA
Chg | Type | More | # | Allele
freq | Pop | Total
sample | More |
|---|
HapMap Linkage Disequilibrium report for NPBWR2 (62737173 - 62738524 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV): 1 variation for NPBWR2
1 CNV: 4108 | SABiosciences Cancer Mutation PCR Assays |
| | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing NPBWR2 |
|
Disorders
/ Diseases
for NPBWR2 gene
(in which this Gene is Involved, According to MalaCards,
OMIM, UniProtKB,
the University of Copenhagen DISEASES
database,
Genatlas, GeneTests,
GAD,
HuGE Navigator,
and/or TGDB.)
About This Section
|
NPBWR2 for disorders About GeneDecksing
OMIM gene information: 600731
OMIM disorders: --
4  diseases for NPBWR2: About MalaCardsadrenocortical carcinoma cryptorchidism hermaphroditism carcinoma
1 disease from the University of Copenhagen DISEASES database for NPBWR2:Cryptorchidism Export disorders for NPBWR2 gene to outside databases
|
Publications
for NPBWR2 gene (in
PubMed.
Associations of this gene to articles via
1Entrez Gene,
2UniProtKB/Swiss-Prot,
3HGNC,
4GAD,
5PharmGKB,
6HMDB,
7DrugBank,
8UniProtKB/TrEMBL,
9 Novoseek, and/or
10fRNAdb)
About This Section
|
PubMed articles for NPBWR2 gene, integrated from 9 sources (see all 23):
(articles sorted by number of sources associating them with NPBWR2) | |  | Utopia: connect your pdf to the dynamic
world of online information |
- Identification of natural ligands for the orphan G protein-coupled receptors GPR7 and GPR8. (PubMed id 12401809)1, 2, 3, 9 Brezillon S.... Parmentier M. (2003)
- Characterization of a family of endogenous neuropeptide ligands for the G protein-coupled receptors GPR7 and GPR8. (PubMed id 12719537)1, 2, 9 Tanaka H.... Yanagisawa M. (2003)
- The cloning and chromosomal mapping of two novel human opioid- somatostatin-like receptor genes, GPR7 and GPR8, expressed in discrete areas of the brain. (PubMed id 7590751)1, 2, 9 O'Dowd B.F.... George S.R. (1995)
- The DNA sequence and comparative analysis of human chromosome 20. (PubMed id 11780052)1, 2 Deloukas P....Rogers J. (2001)
- Identification of neuropeptide W as the endogenous ligand for orphan G-protein-coupled receptors GPR7 and GPR8. (PubMed id 12130646)1, 9 Shimomura Y.... Fujino M. (2002)
- Dissociation of heterotrimeric g proteins in cells. (PubMed id 18577758)1 Lambert N.A. (2008)
- Reviews in molecular biology and biotechnology: transmembrane signaling by G protein-coupled receptors. (PubMed id 18240029)1 Luttrell L.M. (2008)
- hORFeome v3.1: a resource of human open reading frames representing over 10,000 human genes. (PubMed id 17207965)1 Lamesch P.... Vidal M. (2007)
- The consensus coding sequences of human breast and colorectal cancers. (PubMed id 16959974)2 Sjoeblom T.... Velculescu V.E. (2006)
- G protein receptors 7 and 8 are expressed in human adrenocortical cells, and their endogenous ligands neuropeptides B and w enhance cortisol secretion by activating adenylate cyclase- and phospholipase C-dependent signaling cascades. (PubMed id 15797961)1 Mazzocchi G....Nussdorfer G.G. (2005)
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External Searches for NPBWR2 gene
(in PubMed,
OMIM, and NCBI Bookshelf)
About This Section
|
|
Genome Databases showing NPBWR2 gene
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
Kegg,
and/or
H-InvDB)
About This Section
|
|
Other Databases showing NPBWR2 gene
(According to HUGE)
About This Section
| -- |
Specialized Databases showing NPBWR2 gene(According to PharmGKB,
ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section
|
| Name | Description |
| PharmGKB entry for NPBWR2 | Pharmacogenomics, SNPs, Pathways |
|
| | |
About This Section
| Patent Information for NPBWR2 gene:
Search GeneIP for patents involving NPBWR2
GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search
|
Products
for NPBWR2 gene(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or
Enzo Life Sciences),
In Situ Hybridization Assays from
Advanced Cell Diagnostics
About This Section
|
 | |
 | |
 |
| | |  | Browse OriGene Antibodies | | OriGene shRNA RFP for NPBWR2 | | | OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for NPBWR2 | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for NPBWR2 | | | OriGene Protein Over-expression Lysate for NPBWR2 | | Browse OriGene Fluorogenic Cell Assay Kits | | | OriGene siRNA for NPBWR2 | | Browse 3'-UTR reporter clones for miRNA target validation | | | OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for NPBWR2 | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for NPBWR2 | | | Browse OriGene GFP tagged cDNA clones in CMV expression vector | | Browse OriGene MicroRNA Expression Plasmids | | | Browse OriGene basic RS shRNAs | | Browse OriGene validated miRNA SYBR primer pairs | | | Browse OriGene full length recombinant human proteins expressed in human HEK293 cells | | OriGene custom cloning services - gene synthesis, subcloning, mutagenesis,
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| | | QIAGEN Custom miScript Target Protector blocks miRNA-binding site of in human, mouse, rat NPBWR2 | | | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing NPBWR2 | | | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat NPBWR2 | | | QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat NPBWR2 | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat NPBWR2 | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat NPBWR2 |
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| |  |  |  |  | | | | |
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 | | | NPBWR2 Proteins, Antibodies, CLIAs, and ELISAs |
| | | | | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for NPBWR2 |
|  |  |  | | | | ThermoFisher Antibodies for NPBWR2 |
| | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat NPBWR2 |
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