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Aliases for NOX4 Gene

Aliases for NOX4 Gene

  • NADPH Oxidase 4 2 3
  • RENOX 3 4 6
  • Kidney Superoxide-Producing NADPH Oxidase 3 4
  • Renal NAD(P)H-Oxidase 3 4
  • Kidney Oxidase-1 3 4
  • KOX-1 3 4
  • EC 1.6.3.- 4
  • EC 1.6.3 63
  • KOX 3

External Ids for NOX4 Gene

Previous GeneCards Identifiers for NOX4 Gene

  • GC11M091382
  • GC11M090606
  • GC11M089244
  • GC11M088747
  • GC11M088699
  • GC11M089057
  • GC11M085294

Summaries for NOX4 Gene

Entrez Gene Summary for NOX4 Gene

  • This gene encodes a member of the NOX-family of enzymes that functions as the catalytic subunit the NADPH oxidase complex. The encoded protein is localized to non-phagocytic cells where it acts as an oxygen sensor and catalyzes the reduction of molecular oxygen to various reactive oxygen species (ROS). The ROS generated by this protein have been implicated in numerous biological functions including signal transduction, cell differentiation and tumor cell growth. A pseudogene has been identified on the other arm of chromosome 11. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Jan 2009]

GeneCards Summary for NOX4 Gene

NOX4 (NADPH Oxidase 4) is a Protein Coding gene. Diseases associated with NOX4 include leprechaunism. Among its related pathways are PAK Pathway and Apoptotic Pathways in Synovial Fibroblasts. GO annotations related to this gene include nucleotide binding and heme binding. An important paralog of this gene is NOX5.

UniProtKB/Swiss-Prot for NOX4 Gene

  • Constitutive NADPH oxidase which generates superoxide intracellularly upon formation of a complex with CYBA/p22phox. Regulates signaling cascades probably through phosphatases inhibition. May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium channel and HIF1A activity. May regulate insulin signaling cascade. May play a role in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB. May produce superoxide in the nucleus and play a role in regulating gene expression upon cell stimulation. Isoform 3 is not functional. Isoform 5 and isoform 6 display reduced activity

  • Isoform 4: Involved in redox signaling in vascular cells. Constitutively and NADPH-dependently generates reactive oxygen species (ROS). Modulates the nuclear activation of ERK1/2 and the ELK1 transcription factor, and is capable of inducing nuclear DNA damage. Displays an increased activity relative to isoform 1

Gene Wiki entry for NOX4 Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for NOX4 Gene

Genomics for NOX4 Gene

Regulatory Elements for NOX4 Gene

Genomic Location for NOX4 Gene

Start:
89,324,353 bp from pter
End:
89,589,611 bp from pter
Size:
265,259 bases
Orientation:
Minus strand

Genomic View for NOX4 Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for NOX4 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for NOX4 Gene

Proteins for NOX4 Gene

  • Protein details for NOX4 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q9NPH5-NOX4_HUMAN
    Recommended name:
    NADPH oxidase 4
    Protein Accession:
    Q9NPH5
    Secondary Accessions:
    • A8K715
    • B7Z520
    • E7EMD7
    • Q5K3R4
    • Q5K3R5
    • Q5K3R6
    • Q5K3R8
    • Q7Z7G3
    • Q86V92

    Protein attributes for NOX4 Gene

    Size:
    578 amino acids
    Molecular mass:
    66932 Da
    Quaternary structure:
    • Interacts with protein disulfide isomerase (By similarity). Interacts with, relocalizes and stabilizes CYBA/p22phox. Interacts with TLR4.

    Alternative splice isoforms for NOX4 Gene

neXtProt entry for NOX4 Gene

Proteomics data for NOX4 Gene at MOPED

Selected DME Specific Peptides for NOX4 Gene

Post-translational modifications for NOX4 Gene

  • Isoform 3 and isoform 4 are N-glycosylated. Isoform 4 glycosylation is required for its proper function.
  • Glycosylation at Asn133 and Asn230
  • Modification sites at PhosphoSitePlus

Antibody Products

Domains for NOX4 Gene

Suggested Antigen Peptide Sequences for NOX4 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

Q9NPH5

UniProtKB/Swiss-Prot:

NOX4_HUMAN :
  • Q9NPH5
Domain:
  • Contains 1 FAD-binding FR-type domain.
  • Contains 1 ferric oxidoreductase domain.
genes like me logo Genes that share domains with NOX4: view

No data available for Gene Families for NOX4 Gene

Function for NOX4 Gene

Molecular function for NOX4 Gene

UniProtKB/Swiss-Prot EnzymeRegulation: Inhibited by plumbagin (By similarity). Activated by phorbol 12-myristate 13-acetate (PMA). Activated by insulin. Inhibited by diphenylene iodonium.
UniProtKB/Swiss-Prot Function: Constitutive NADPH oxidase which generates superoxide intracellularly upon formation of a complex with CYBA/p22phox. Regulates signaling cascades probably through phosphatases inhibition. May function as an oxygen sensor regulating the KCNK3/TASK-1 potassium channel and HIF1A activity. May regulate insulin signaling cascade. May play a role in apoptosis, bone resorption and lipolysaccharide-mediated activation of NFKB. May produce superoxide in the nucleus and play a role in regulating gene expression upon cell stimulation. Isoform 3 is not functional. Isoform 5 and isoform 6 display reduced activity
UniProtKB/Swiss-Prot Function: Isoform 4: Involved in redox signaling in vascular cells. Constitutively and NADPH-dependently generates reactive oxygen species (ROS). Modulates the nuclear activation of ERK1/2 and the ELK1 transcription factor, and is capable of inducing nuclear DNA damage. Displays an increased activity relative to isoform 1
UniProtKB/Swiss-Prot Induction: By 7-ketocholesterol (at protein level).

Enzyme Numbers (IUBMB) for NOX4 Gene

Gene Ontology (GO) - Molecular Function for NOX4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000166 nucleotide binding TAS 10869423
GO:0005515 protein binding --
GO:0009055 electron carrier activity TAS 10869423
GO:0016174 NAD(P)H oxidase activity TAS 10869423
GO:0016175 superoxide-generating NADPH oxidase activity IEA --
genes like me logo Genes that share ontologies with NOX4: view
genes like me logo Genes that share phenotypes with NOX4: view

Animal Models for NOX4 Gene

MGI Knock Outs for NOX4:

Animal Model Products

CRISPR Products

miRNA for NOX4 Gene

miRTarBase miRNAs that target NOX4

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for NOX4

In Situ Assay Products

Flow Cytometry Products

No data available for Transcription Factor Targeting and HOMER Transcription for NOX4 Gene

Localization for NOX4 Gene

Subcellular locations from UniProtKB/Swiss-Prot for NOX4 Gene

Endoplasmic reticulum membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Cell junction, focal adhesion. Note=May localize to plasma membrane and focal adhesions. According to PubMed:15927447, may also localize to the nucleus.
Isoform 4: Nucleus. Nucleus, nucleolus.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for NOX4 Gene COMPARTMENTS Subcellular localization image for NOX4 gene
Compartment Confidence
endoplasmic reticulum 5
plasma membrane 5
nucleus 4
extracellular 2
mitochondrion 2
cytoskeleton 1

Gene Ontology (GO) - Cellular Components for NOX4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0001725 stress fiber IEA --
GO:0005634 nucleus --
GO:0005730 nucleolus IEA --
GO:0005739 mitochondrion IEA --
GO:0005783 endoplasmic reticulum --
genes like me logo Genes that share ontologies with NOX4: view

Pathways for NOX4 Gene

genes like me logo Genes that share pathways with NOX4: view

Pathways by source for NOX4 Gene

PCR Array Products

Gene Ontology (GO) - Biological Process for NOX4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000902 cell morphogenesis ISS 10869423
GO:0001666 response to hypoxia IEA --
GO:0006801 superoxide metabolic process IDA 18760347
GO:0006954 inflammatory response TAS 10869423
GO:0007568 aging --
genes like me logo Genes that share ontologies with NOX4: view

Compounds for NOX4 Gene

(3) HMDB Compounds for NOX4 Gene

Compound Synonyms Cas Number PubMed IDs
FAD
  • 1H-Purin-6-amine flavin dinucleotide
146-14-5
Heme
  • (protoporphyrinato)iron
14875-96-8
NADPH
  • 2'-(dihydrogen phosphate) 5'-(trihydrogen pyrophosphate) Adenosine 5'-ester with 1,4-dihydro-1-b-D-ribofuranosylnicotinamide
53-57-6

(13) Novoseek inferred chemical compound relationships for NOX4 Gene

Compound -log(P) Hits PubMed IDs
nadph 89.8 100
diphenyleneiodonium 81.1 3
apocynin 78.3 2
superoxide 69.2 31
oxygen 69.1 29
genes like me logo Genes that share compounds with NOX4: view

Transcripts for NOX4 Gene

Unigene Clusters for NOX4 Gene

NADPH oxidase 4:
Representative Sequences:

CRISPR Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for NOX4

Primer Products

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for NOX4 Gene

No ASD Table

Relevant External Links for NOX4 Gene

GeneLoc Exon Structure for
NOX4
ECgene alternative splicing isoforms for
NOX4

Expression for NOX4 Gene

mRNA expression in normal human tissues for NOX4 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for NOX4 Gene

This gene is overexpressed in Kidney - Cortex (10.8), Artery - Aorta (10.8), Artery - Tibial (5.7), and Artery - Coronary (4.1).

SOURCE GeneReport for Unigene cluster for NOX4 Gene Hs.371036

mRNA Expression by UniProt/SwissProt for NOX4 Gene

Q9NPH5-NOX4_HUMAN
Tissue specificity: Expressed by distal tubular cells in kidney cortex and in endothelial cells (at protein level). Widely expressed. Strongly expressed in kidney and to a lower extent in heart, adipocytes, hepatoma, endothelial cells, skeletal muscle, brain, several brain tumor cell lines and airway epithelial cells.
genes like me logo Genes that share expressions with NOX4: view

In Situ Assay Products

No data available for Protein expression for NOX4 Gene

Orthologs for NOX4 Gene

This gene was present in the common ancestor of animals.

Orthologs for NOX4 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia NOX4 35
  • 99.54 (n)
  • 99.48 (a)
NOX4 36
  • 99 (a)
OneToOne
cow
(Bos Taurus)
Mammalia NOX4 35
  • 91.7 (n)
  • 94.29 (a)
NOX4 36
  • 94 (a)
OneToOne
dog
(Canis familiaris)
Mammalia NOX4 35
  • 89.09 (n)
  • 89.55 (a)
NOX4 36
  • 83 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Nox4 35
  • 86.97 (n)
  • 90.83 (a)
Nox4 16
Nox4 36
  • 91 (a)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia NOX4 36
  • 89 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia NOX4 36
  • 87 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Nox4 35
  • 86.97 (n)
  • 90.14 (a)
chicken
(Gallus gallus)
Aves NOX4 35
  • 77.14 (n)
  • 79.72 (a)
NOX4 36
  • 79 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia NOX4 36
  • 76 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia nox4 35
  • 67.71 (n)
  • 67.19 (a)
zebrafish
(Danio rerio)
Actinopterygii CABZ01079296.1 36
  • 68 (a)
OneToMany
NOX4 (1 of 2) 36
  • 67 (a)
OneToMany
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP003772 35
  • 44.59 (n)
  • 33.4 (a)
sea squirt
(Ciona savignyi)
Ascidiacea -- 36
  • 31 (a)
OneToOne
Species with no ortholog for NOX4:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • fruit fly (Drosophila melanogaster)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for NOX4 Gene

ENSEMBL:
Gene Tree for NOX4 (if available)
TreeFam:
Gene Tree for NOX4 (if available)

Paralogs for NOX4 Gene

Paralogs for NOX4 Gene

Selected SIMAP similar genes for NOX4 Gene using alignment to 6 proteins:

Pseudogenes.org Pseudogenes for NOX4 Gene

genes like me logo Genes that share paralogs with NOX4: view

Variants for NOX4 Gene

Sequence variations from dbSNP and Humsavar for NOX4 Gene

SNP ID Clin Chr 11 pos Sequence Context AA Info Type MAF
rs172742 -- 89,424,165(+) AACAT(A/G)TATCT intron-variant
rs178434 -- 89,389,243(+) GACAT(C/G)TATGA intron-variant
rs178435 -- 89,381,316(+) cctcc(G/T)agccc intron-variant
rs178436 -- 89,359,866(-) GTACC(G/T)TTTGT intron-variant
rs188824 -- 89,380,893(+) ATTAT(A/G)AAAAC intron-variant

Structural Variations from Database of Genomic Variants (DGV) for NOX4 Gene

Variant ID Type Subtype PubMed ID
esv32702 CNV Gain+Loss 17666407
nsv469976 CNV Gain 18288195
nsv468781 CNV Gain 19166990
dgv165n27 CNV Loss 19166990
nsv898088 CNV Gain 21882294
esv1095170 CNV Deletion 17803354
esv269866 CNV Insertion 20981092
esv272386 CNV Insertion 20981092
esv267878 CNV Insertion 20981092
nsv898089 CNV Loss 21882294
nsv468785 CNV Gain 19166990
nsv898090 CNV Loss 21882294
dgv1254n71 CNV Loss 21882294
nsv898094 CNV Gain 21882294

Relevant External Links for NOX4 Gene

HapMap Linkage Disequilibrium report
NOX4

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for NOX4 Gene

Disorders for NOX4 Gene

MalaCards: The human disease database

MalaCards: The human disease database. (1) Diseases for NOX4 Gene including...

Search for NOX4 Gene in MalaCards »

(3) University of Copenhagen DISEASES for NOX4 Gene

(8) Novoseek inferred disease relationships for NOX4 Gene

Disease -log(P) Hits PubMed IDs
vascular diseases 44.1 1
hypertension pulmonary 28.9 1
atherosclerosis 18.8 1
insulin resistance 7.75 1
tumors 6.87 7

Relevant External Links for NOX4

Genetic Association Database (GAD)
NOX4
Human Genome Epidemiology (HuGE) Navigator
NOX4
genes like me logo Genes that share disorders with NOX4: view

No data available for UniProtKB/Swiss-Prot for NOX4 Gene

Publications for NOX4 Gene

  1. Identification of renox, an NAD(P)H oxidase in kidney. (PMID: 10869423) Geiszt M. … Leto T.L. (Proc. Natl. Acad. Sci. U.S.A. 2000) 3 4 23
  2. A novel superoxide-producing NAD(P)H oxidase in kidney. (PMID: 11032835) Shiose A. … Sumimoto H. (J. Biol. Chem. 2001) 3 4 23
  3. The NAD(P)H oxidase homolog Nox4 modulates insulin-stimulated generation of H2O2 and plays an integral role in insulin signal transduction. (PMID: 14966267) Mahadev K. … Goldstein B.J. (Mol. Cell. Biol. 2004) 3 4 23
  4. Reactive oxygen species produced by NAD(P)H oxidase inhibit apoptosis in pancreatic cancer cells. (PMID: 15155719) Vaquero E.C. … Gukovskaya A.S. (J. Biol. Chem. 2004) 3 4 23
  5. NAD(P)H oxidase Nox-4 mediates 7-ketocholesterol-induced endoplasmic reticulum stress and apoptosis in human aortic smooth muscle cells. (PMID: 15572675) Pedruzzi E. … Ogier-Denis E. (Mol. Cell. Biol. 2004) 3 4 23

Products for NOX4 Gene

Sources for NOX4 Gene

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