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Aliases for NOSIP Gene

Aliases for NOSIP Gene

  • Nitric Oxide Synthase Interacting Protein 2 3 5
  • RING-Type E3 Ubiquitin Transferase NOSIP 3 4
  • E3 Ubiquitin-Protein Ligase NOSIP 3 4
  • ENOS-Interacting Protein 3 4
  • Nitric Oxide Synthase-Interacting Protein 3
  • EC 2.3.2.27 4
  • CGI-25 3

External Ids for NOSIP Gene

Previous GeneCards Identifiers for NOSIP Gene

  • GC19M050717
  • GC19M050427
  • GC19M054734
  • GC19M054751
  • GC19M054752
  • GC19M054753
  • GC19M050058
  • GC19M046436

Summaries for NOSIP Gene

Entrez Gene Summary for NOSIP Gene

  • The protein encoded by this gene may modulate the activity and localization of nitric oxide synthase (endothelial and neuronal) and thus nitric oxide production. Alternative splicing results in multiple transcript variants that encode the same protein. [provided by RefSeq, Aug 2012]

GeneCards Summary for NOSIP Gene

NOSIP (Nitric Oxide Synthase Interacting Protein) is a Protein Coding gene. Among its related pathways are eNOS activation and regulation and Nanog in Mammalian ESC Pluripotency. GO annotations related to this gene include poly(A) RNA binding.

UniProtKB/Swiss-Prot for NOSIP Gene

  • E3 ubiquitin-protein ligase that is essential for proper development of the forebrain, the eye, and the face. Catalyzes monoubiquitination of serine/threonine-protein phosphatase 2A (PP2A) catalytic subunit PPP2CA/PPP2CB (By similarity). Negatively regulates nitric oxide production by inducing NOS1 and NOS3 translocation to actin cytoskeleton and inhibiting their enzymatic activity (PubMed:11149895, PubMed:15548660, PubMed:16135813).

Gene Wiki entry for NOSIP Gene

Additional gene information for NOSIP Gene

No data available for CIViC summary , Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for NOSIP Gene

Genomics for NOSIP Gene

Regulatory Elements for NOSIP Gene

Enhancers for NOSIP Gene
GeneHancer Identifier Enhancer Score Enhancer Sources Gene-Enhancer Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor Binding Sites within enhancer Gene Targets for Enhancer
GH19H049567 1.5 Ensembl ENCODE dbSUPER 28.4 +20.7 20697 4 PKNOX1 FOXA2 ARNT ARID4B SIN3A FEZF1 ZNF2 YY1 ZNF121 ZNF143 PRR12 NOSIP SNORD35B PRRG2 POLD1 NUP62 PTOV1-AS2 SNORD35A FCGRT MIR150
GH19H049552 1.3 Ensembl ENCODE dbSUPER 28.5 +34.9 34913 5 PKNOX1 ARID4B MIXL1 EGR2 ZNF362 ZNF623 NR2F2 ZBTB33 ZNF24 KDM1A NOSIP PRR12 FCGRT RCN3 PRRG2 MIR150 SNORD35B RPS11 SNORD35A SNORD34
GH19H049558 1.2 Ensembl ENCODE dbSUPER 28.5 +29.5 29538 4 BCOR TFAP4 NRF1 ZMYM3 MNT ZNF664 ZFHX2 FOXK2 EGR1 ADNP PRR12 NOSIP RCN3 FCGRT MIR150 RPS11 SNORD35B SNORD35A SNORD34 SNORD32A
GH19H048958 2.1 FANTOM5 Ensembl ENCODE dbSUPER 11.4 +626.2 626233 10 MLX ZFP64 DMAP1 YY1 SLC30A9 ZNF143 SP3 ZC3H11A NFYC MEF2D NUP62 PRR12 ENSG00000269321 RUVBL2 PPP1R15A TSKS ENSG00000268047 IRF3 PIH1D1 CPT1C
GH19H049589 1.7 FANTOM5 ENCODE dbSUPER 12.1 -1.8 -1781 5 HDGF PKNOX1 ARNT ARID4B SIN3A DMAP1 ZNF2 YY1 SLC30A9 ZNF143 NUP62 PRR12 POLD1 ENSG00000268047 IRF3 ZNF473 PNKP ALDH16A1 SNORD35A SNORD35B
- Elite enhancer and/or Elite enhancer-gene association Download GeneHancer data dump

Enhancers around NOSIP on UCSC Golden Path with GeneCards custom track

Promoters for NOSIP Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters
ENSR00000110924 -1638 3401 HDGF PKNOX1 ARNT ARID4B SIN3A FEZF1 DMAP1 ZNF2 YY1 SLC30A9

Genomic Location for NOSIP Gene

Chromosome:
19
Start:
49,555,468 bp from pter
End:
49,590,262 bp from pter
Size:
34,795 bases
Orientation:
Minus strand

Genomic View for NOSIP Gene

Genes around NOSIP on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
NOSIP Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for NOSIP Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for NOSIP Gene

Proteins for NOSIP Gene

  • Protein details for NOSIP Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q9Y314-NOSIP_HUMAN
    Recommended name:
    Nitric oxide synthase-interacting protein
    Protein Accession:
    Q9Y314
    Secondary Accessions:
    • Q96FD2

    Protein attributes for NOSIP Gene

    Size:
    301 amino acids
    Molecular mass:
    33172 Da
    Quaternary structure:
    • Interacts with NOS1 and NOS3 (PubMed:11149895, PubMed:15548660). Interacts with PP2A holoenzyme, containing PPP2CA, PPP2CB, PPP2R1A and PPP2R2A subunits (By similarity).

neXtProt entry for NOSIP Gene

Post-translational modifications for NOSIP Gene

  • Ubiquitination at posLast=117117, posLast=157157, posLast=253253, and Lys294
  • Modification sites at PhosphoSitePlus

Antibody Products

  • Abcam antibodies for NOSIP

No data available for DME Specific Peptides for NOSIP Gene

Domains & Families for NOSIP Gene

Gene Families for NOSIP Gene

Human Protein Atlas (HPA):
  • Predicted intracellular proteins

Protein Domains for NOSIP Gene

Suggested Antigen Peptide Sequences for NOSIP Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

Q9Y314

UniProtKB/Swiss-Prot:

NOSIP_HUMAN :
  • The U-box-like region is a truncated U-box domain. It is unknown whether it is functional or not.
  • Belongs to the NOSIP family.
Domain:
  • The U-box-like region is a truncated U-box domain. It is unknown whether it is functional or not.
Family:
  • Belongs to the NOSIP family.
genes like me logo Genes that share domains with NOSIP: view

Function for NOSIP Gene

Molecular function for NOSIP Gene

UniProtKB/Swiss-Prot CatalyticActivity:
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.
UniProtKB/Swiss-Prot Function:
E3 ubiquitin-protein ligase that is essential for proper development of the forebrain, the eye, and the face. Catalyzes monoubiquitination of serine/threonine-protein phosphatase 2A (PP2A) catalytic subunit PPP2CA/PPP2CB (By similarity). Negatively regulates nitric oxide production by inducing NOS1 and NOS3 translocation to actin cytoskeleton and inhibiting their enzymatic activity (PubMed:11149895, PubMed:15548660, PubMed:16135813).

Enzyme Numbers (IUBMB) for NOSIP Gene

Phenotypes From GWAS Catalog for NOSIP Gene

Gene Ontology (GO) - Molecular Function for NOSIP Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0003723 RNA binding IDA 22658674
GO:0004842 ubiquitin-protein transferase activity IEA --
GO:0005515 protein binding IPI 11149895
GO:0016740 transferase activity IEA --
GO:0061630 ubiquitin protein ligase activity IBA --
genes like me logo Genes that share ontologies with NOSIP: view
genes like me logo Genes that share phenotypes with NOSIP: view

Animal Model Products

CRISPR Products

miRNA for NOSIP Gene

miRTarBase miRNAs that target NOSIP

Inhibitory RNA Products

Clone Products

  • Addgene plasmids for NOSIP
  • Applied Biological Materials Clones for NOSIP
  • Vectors and viruses for ORF, Lenti, Retro, Adenovirus, AAV, and more

No data available for Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for NOSIP Gene

Localization for NOSIP Gene

Subcellular locations from UniProtKB/Swiss-Prot for NOSIP Gene

Cytoplasm. Nucleus. Note=Translocates from nucleus to cytoplasm in the G2 phase of the cell cycle (PubMed:16135813).

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for NOSIP gene
Compartment Confidence
nucleus 5
cytosol 5
golgi apparatus 4
plasma membrane 1
extracellular 1
peroxisome 1

Subcellular locations from the

Human Protein Atlas (HPA)

Gene Ontology (GO) - Cellular Components for NOSIP Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000139 Golgi membrane TAS --
GO:0005634 nucleus IDA 15548660
GO:0005737 cytoplasm IEA,IDA 15548660
GO:0005829 cytosol TAS --
genes like me logo Genes that share ontologies with NOSIP: view

Pathways & Interactions for NOSIP Gene

genes like me logo Genes that share pathways with NOSIP: view

Pathways by source for NOSIP Gene

Interacting Proteins for NOSIP Gene

Selected Interacting proteins: Q9Y314-NOSIP_HUMAN for NOSIP Gene via IID

Gene Ontology (GO) - Biological Process for NOSIP Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0007275 multicellular organism development IEA --
GO:0016567 protein ubiquitination IBA,IEA --
GO:0043086 negative regulation of catalytic activity IDA 11149895
GO:0050999 regulation of nitric-oxide synthase activity IEA,TAS --
GO:0051001 negative regulation of nitric-oxide synthase activity IDA 11149895
genes like me logo Genes that share ontologies with NOSIP: view

No data available for SIGNOR curated interactions for NOSIP Gene

Drugs & Compounds for NOSIP Gene

No Compound Related Data Available

Transcripts for NOSIP Gene

Unigene Clusters for NOSIP Gene

Nitric oxide synthase interacting protein:
Representative Sequences:

CRISPR Products

Inhibitory RNA Products

Clone Products

  • Addgene plasmids for NOSIP
  • Applied Biological Materials Clones for NOSIP
  • Vectors and viruses for ORF, Lenti, Retro, Adenovirus, AAV, and more

Alternative Splicing Database (ASD) splice patterns (SP) for NOSIP Gene

ExUns: 1a · 1b ^ 2 ^ 3 ^ 4a · 4b · 4c ^ 5a · 5b ^ 6 ^ 7a · 7b ^ 8a · 8b · 8c ^ 9a · 9b ^ 10 ^ 11 ^ 12a · 12b
SP1: - - - - - -
SP2: - - - - - - -
SP3: - - - - -
SP4: - - - -
SP5: - - - - - -
SP6: - - - - - - - -
SP7:
SP8: - - - -
SP9: -
SP10: - -

Relevant External Links for NOSIP Gene

GeneLoc Exon Structure for
NOSIP
ECgene alternative splicing isoforms for
NOSIP

Expression for NOSIP Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and CGAP SAGE for NOSIP Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for NOSIP Gene

This gene is overexpressed in Whole Blood (x4.3).

Protein differential expression in normal tissues from HIPED for NOSIP Gene

This gene is overexpressed in Breast (43.4) and Lymph node (7.6).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, MaxQB, and MOPED for NOSIP Gene



Protein tissue co-expression partners for NOSIP Gene

- Elite partner

NURSA nuclear receptor signaling pathways regulating expression of NOSIP Gene:

NOSIP

SOURCE GeneReport for Unigene cluster for NOSIP Gene:

Hs.7236

mRNA Expression by UniProt/SwissProt for NOSIP Gene:

Q9Y314-NOSIP_HUMAN
Tissue specificity: Expressed in heart, brain and lung. Present in endothelial cells (at protein level).

Evidence on tissue expression from TISSUES for NOSIP Gene

  • Nervous system(4.6)
  • Liver(4.5)
  • Lung(3)
  • Muscle(2)
genes like me logo Genes that share expression patterns with NOSIP: view

Primer Products

No data available for Phenotype-based relationships between genes and organs from Gene ORGANizer for NOSIP Gene

Orthologs for NOSIP Gene

This gene was present in the common ancestor of eukaryotes.

Orthologs for NOSIP Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia NOSIP 33 34
  • 98.89 (n)
cow
(Bos Taurus)
Mammalia NOSIP 33 34
  • 91.03 (n)
dog
(Canis familiaris)
Mammalia NOSIP 33 34
  • 88.26 (n)
rat
(Rattus norvegicus)
Mammalia Nosip 33
  • 85.94 (n)
mouse
(Mus musculus)
Mammalia Nosip 33 16 34
  • 85.83 (n)
oppossum
(Monodelphis domestica)
Mammalia NOSIP 34
  • 84 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia -- 34
  • 84 (a)
OneToMany
-- 34
  • 38 (a)
OneToMany
lizard
(Anolis carolinensis)
Reptilia NOSIP 34
  • 78 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia nosip 33
  • 65.88 (n)
MGC75989 33
zebrafish
(Danio rerio)
Actinopterygii nosip 33 34
  • 63.41 (n)
-- 33
fruit fly
(Drosophila melanogaster)
Insecta CG6179 35 33 34
  • 59.8 (n)
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP005654 33
  • 58.97 (n)
worm
(Caenorhabditis elegans)
Secernentea R05G6.4 33 34
  • 48.51 (n)
thale cress
(Arabidopsis thaliana)
eudicotyledons AT1G61620 33
  • 44.29 (n)
rice
(Oryza sativa)
Liliopsida Os03g0279900 33
  • 46.16 (n)
sea squirt
(Ciona savignyi)
Ascidiacea -- 34
  • 50 (a)
OneToOne
Species where no ortholog for NOSIP was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • chicken (Gallus gallus)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for NOSIP Gene

ENSEMBL:
Gene Tree for NOSIP (if available)
TreeFam:
Gene Tree for NOSIP (if available)

Paralogs for NOSIP Gene

No data available for Paralogs for NOSIP Gene

Variants for NOSIP Gene

Sequence variations from dbSNP and Humsavar for NOSIP Gene

SNP ID Clin Chr 19 pos Sequence Context AA Info Type
rs1000145770 -- 49,572,268(+) GCCTG(C/G)CTAAT intron-variant
rs1000148676 -- 49,562,154(+) TTCAC(C/T)CTTGT intron-variant
rs1000256329 -- 49,566,342(+) TCTCA(C/T)TCTGT intron-variant
rs1000273297 -- 49,567,254(+) AGTAG(C/T)TGGGA intron-variant
rs1000332089 -- 49,580,610(+) TTCCG(C/T)CCAGC upstream-variant-2KB

Structural Variations from Database of Genomic Variants (DGV) for NOSIP Gene

Variant ID Type Subtype PubMed ID
nsv1160655 CNV deletion 26073780
nsv469715 CNV gain+loss 16826518
nsv828578 CNV loss 20364138
nsv833862 CNV loss 17160897
nsv833863 CNV loss 17160897
nsv833864 CNV gain 17160897
nsv833865 CNV gain+loss 17160897
nsv953600 CNV deletion 24416366
nsv9739 CNV gain+loss 18304495

Variation tolerance for NOSIP Gene

Residual Variation Intolerance Score: 26.6% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 0.42; 9.29% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for NOSIP Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
NOSIP

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for NOSIP Gene

Disorders for NOSIP Gene

Relevant External Links for NOSIP

Atlas of Genetics and Cytogenetics in Oncology and Haematology:
NOSIP

No disorders were found for NOSIP Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for NOSIP Gene

Publications for NOSIP Gene

  1. Cell cycle-regulated inactivation of endothelial NO synthase through NOSIP-dependent targeting to the cytoskeleton. (PMID: 16135813) Schleicher M … Oess S (Molecular and cellular biology 2005) 3 4 22 60
  2. NOSIP, a novel modulator of endothelial nitric oxide synthase activity. (PMID: 11149895) Dedio J … Müller-Esterl W (FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2001) 2 3 4 60
  3. Identification of novel human genes evolutionarily conserved in Caenorhabditis elegans by comparative proteomics. (PMID: 10810093) Lai CH … Lin W (Genome research 2000) 2 3 4 60
  4. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PMID: 15489334) Gerhard DS … MGC Project Team (Genome research 2004) 3 4 60
  5. Nitric oxide synthase (NOS)-interacting protein interacts with neuronal NOS and regulates its distribution and activity. (PMID: 15548660) Dreyer J … Kuner R (The Journal of neuroscience : the official journal of the Society for Neuroscience 2004) 3 4 60

Products for NOSIP Gene

  • Addgene plasmids for NOSIP

Sources for NOSIP Gene

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