Free for academic non-profit institutions. Other users need a Commercial license

Aliases for NOS3 Gene

Aliases for NOS3 Gene

  • Nitric Oxide Synthase 3 2 3 5
  • Nitric Oxide Synthase 3 (Endothelial Cell) 2 3
  • Constitutive NOS 3 4
  • Endothelial NOS 3 4
  • EC 1.14.13.39 4 63
  • NOS Type III 3 4
  • EC-NOS 3 4
  • NOSIII 3 4
  • CNOS 3 4
  • ENOS 3 4
  • Nitric Oxide Synthase 3 Transcript Variant ENOS-Delta20-21 3
  • Nitric Oxide Synthase 3 Transcript Variant ENOS-Delta20 3
  • Nitric Oxide Synthase 3 Transcript Variant ENOS-Delta21 3
  • Endothelial Nitric Oxide Synthase 2
  • ECNOS 3

External Ids for NOS3 Gene

Previous GeneCards Identifiers for NOS3 Gene

  • GC07P148937
  • GC07P150005
  • GC07P150082
  • GC07P150079
  • GC07P150125
  • GC07P150319
  • GC07P150688
  • GC07P144500

Summaries for NOS3 Gene

Entrez Gene Summary for NOS3 Gene

  • Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. Nitric oxide is synthesized from L-arginine by nitric oxide synthases. Variations in this gene are associated with susceptibility to coronary spasm. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]

GeneCards Summary for NOS3 Gene

NOS3 (Nitric Oxide Synthase 3) is a Protein Coding gene. Diseases associated with NOS3 include preeclampsia/eclampsia 1 and stroke, ischemic. Among its related pathways are Platelet activation, signaling and aggregation and Sertoli-Sertoli Cell Junction Dynamics. GO annotations related to this gene include oxidoreductase activity and iron ion binding. An important paralog of this gene is NOS1.

UniProtKB/Swiss-Prot for NOS3 Gene

  • Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.

  • Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1.

Tocris Summary for NOS3 Gene

  • Endothelial nitric oxide synthase (eNOS), along with inducible nitric oxide synthase (iNOS) and neuronal nitric oxide synthase (nNOS), catalyze nitric oxide and L-citrulline generation from L-arginine and molecular oxygen. eNOS is activated at calcium concentrations over 100 nM.

Gene Wiki entry for NOS3 Gene

No data available for PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for NOS3 Gene

Genomics for NOS3 Gene

Regulatory Elements for NOS3 Gene

Genomic Location for NOS3 Gene

Chromosome:
7
Start:
150,990,995 bp from pter
End:
151,014,599 bp from pter
Size:
23,605 bases
Orientation:
Plus strand

Genomic View for NOS3 Gene

Genes around NOS3 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
NOS3 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for NOS3 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for NOS3 Gene

Proteins for NOS3 Gene

  • Protein details for NOS3 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P29474-NOS3_HUMAN
    Recommended name:
    Nitric oxide synthase, endothelial
    Protein Accession:
    P29474
    Secondary Accessions:
    • A0S0A7
    • A0S0A8
    • A8KA63
    • B2RCQ1
    • E9PFR2
    • Q13662
    • Q14251
    • Q14434
    • Q548C1
    • Q6GSL5
    • Q9UDC6

    Protein attributes for NOS3 Gene

    Size:
    1203 amino acids
    Molecular mass:
    133289 Da
    Cofactor:
    Name=5,6,7,8-tetrahydrobiopterin; Xref=ChEBI:CHEBI:15372;
    Cofactor:
    Name=FAD; Xref=ChEBI:CHEBI:57692;
    Cofactor:
    Name=FMN; Xref=ChEBI:CHEBI:58210;
    Cofactor:
    Name=heme; Xref=ChEBI:CHEBI:30413;
    Quaternary structure:
    • Homodimer. Interacts with NOSIP and NOSTRIN.

    Three dimensional structures from OCA and Proteopedia for NOS3 Gene

    Alternative splice isoforms for NOS3 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for NOS3 Gene

Proteomics data for NOS3 Gene at MOPED

Post-translational modifications for NOS3 Gene

  • Phosphorylation by AMPK at Ser-1177 in the presence of Ca(2+)-calmodulin (CaM) activates activity. In absence of Ca(2+)-calmodulin, AMPK also phosphorylates Thr-495, resulting in inhibition of activity (By similarity). Phosphorylation of Ser-114 by CDK5 reduces activity.
  • Ubiquitination at Lys 1035
  • Modification sites at PhosphoSitePlus

Antibody Products

  • Cell Signaling Technology (CST) Antibodies for NOS3 (eNOS)

Domains & Families for NOS3 Gene

Gene Families for NOS3 Gene

IUPHAR :

Suggested Antigen Peptide Sequences for NOS3 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

P29474

UniProtKB/Swiss-Prot:

NOS3_HUMAN :
  • Contains 1 FAD-binding FR-type domain.
  • Belongs to the NOS family.
Domain:
  • Contains 1 FAD-binding FR-type domain.
  • Contains 1 flavodoxin-like domain.
Family:
  • Belongs to the NOS family.
genes like me logo Genes that share domains with NOS3: view

Function for NOS3 Gene

Molecular function for NOS3 Gene

GENATLAS Biochemistry:
nitric oxide synthase 3,constitutively expressed,endothelial,also expressed in cardiac myocytes,regulating vascular tone,overexpressed in squamous hyperplasia,dysplasia and squamous cell carcinoma of the head and neck,putative susceptibility factor for late onset Alzheimer disease
UniProtKB/Swiss-Prot CatalyticActivity:
2 L-arginine + 3 NADPH + 4 O(2) = 2 L-citrulline + 2 nitric oxide + 3 NADP(+) + 4 H(2)O.
UniProtKB/Swiss-Prot EnzymeRegulation:
Stimulated by calcium/calmodulin. Inhibited by NOSIP and NOSTRIN.
UniProtKB/Swiss-Prot Function:
Produces nitric oxide (NO) which is implicated in vascular smooth muscle relaxation through a cGMP-mediated signal transduction pathway. NO mediates vascular endothelial growth factor (VEGF)-induced angiogenesis in coronary vessels and promotes blood clotting through the activation of platelets.
UniProtKB/Swiss-Prot Function:
Isoform eNOS13C: Lacks eNOS activity, dominant-negative form that may down-regulate eNOS activity by forming heterodimers with isoform 1.

Enzyme Numbers (IUBMB) for NOS3 Gene

Gene Ontology (GO) - Molecular Function for NOS3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005506 iron ion binding IEA --
GO:0020037 heme binding IDA 7488039
genes like me logo Genes that share ontologies with NOS3: view
genes like me logo Genes that share phenotypes with NOS3: view

Animal Models for NOS3 Gene

MGI Knock Outs for NOS3:

Animal Model Products

miRNA for NOS3 Gene

miRTarBase miRNAs that target NOS3

In Situ Assay Products

Flow Cytometry Products

No data available for Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for NOS3 Gene

Localization for NOS3 Gene

Subcellular locations from UniProtKB/Swiss-Prot for NOS3 Gene

Cell membrane. Membrane, caveola. Cytoplasm, cytoskeleton. Golgi apparatus. Note=Specifically associates with actin cytoskeleton in the G2 phase of the cell cycle; which is favored by interaction with NOSIP and results in a reduced enzymatic activity.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for NOS3 Gene COMPARTMENTS Subcellular localization image for NOS3 gene
Compartment Confidence
cytoskeleton 5
cytosol 5
golgi apparatus 5
plasma membrane 5
nucleus 4
extracellular 3
mitochondrion 3
endoplasmic reticulum 2
peroxisome 2
vacuole 1

No data available for Gene Ontology (GO) - Cellular Components for NOS3 Gene

Pathways & Interactions for NOS3 Gene

genes like me logo Genes that share pathways with NOS3: view

Pathways by source for NOS3 Gene

SIGNOR curated interactions for NOS3 Gene

Is activated by:
Is inactivated by:

Gene Ontology (GO) - Biological Process for NOS3 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0001525 angiogenesis IEA --
GO:0001542 ovulation from ovarian follicle IEA --
GO:0001701 in utero embryonic development IEA --
GO:0003057 regulation of the force of heart contraction by chemical signal IEA --
GO:0003100 regulation of systemic arterial blood pressure by endothelin IMP 8999856
genes like me logo Genes that share ontologies with NOS3: view

Drugs & Compounds for NOS3 Gene

(143) Drugs for NOS3 Gene - From: Novoseek, HMDB, FDA Approved Drugs, DGIdb, PharmGKB, DrugBank, and ApexBio

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Tetrahydrobiopterin Approved, Investigational Pharma cofactor, Target 0
Doxorubicin Approved, Investigational Pharma Enzyme, substrate Topo II inhibitor,immunosuppresive antineoplastic antibiotic 1582
Citrulline Approved Nutra Full agonist, Agonist, Target 0
Miconazole Approved, Investigational Pharma Antagonist, Pore Blocker, Target, inhibitor 3026
L-Arginine Approved Nutra Full agonist, Agonist, Target 0

(57) Additional Compounds for NOS3 Gene - From: Novoseek, HMDB, and Tocris

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
nadph
  • 2'-(Dihydrogen phosphate) 5'-(trihydrogen pyrophosphate) Adenosine 5'-ester with 1,4-dihydro-1-b-D-ribofuranosylnicotinamide
  • 2'-(Dihydrogen phosphate) 5'-(trihydrogen pyrophosphate) Adenosine 5'-ester with 1,4-dihydro-1-beta-delta-ribofuranosylnicotinamide
  • Adenosine 5'-(trihydrogen diphosphate) 2'-(dihydrogen phosphate) P'-5'-ester with 1,4-dihydro-1-beta-D-ribofuranosyl-3-pyridinecarboxamide
  • Adenosine 5'-(trihydrogen diphosphate) 2'-(dihydrogen phosphate) P'-5'-ester with 1,4-dihydro-1-beta-delta-ribofuranosyl-3-pyridinecarboxamide
  • b-NADPH
53-57-6
oxygen
  • Dioxygen
  • Molecular oxygen
  • O2
  • Oxygen
  • Oxygen molecule
7782-44-7
Canavanine
  • Canavanin
  • 2-Amino-4-(guanidinooxy)butyric acid
  • L-Canavanine
  • O-((Aminoiminomethyl)amino)homoserine
543-38-4
Homo-L-arginine
  • Homo-L-arginine
  • Homoarginine
  • L-Homoarginine
  • N6-(Aminoiminomethyl)-L-lysine
  • N6-amidino-L-Lysine
156-86-5
Hydrogen Ion
  • Hydrogen cation
  • Hydron
  • Proton

(1) Tocris Compounds for NOS3 Gene

Compound Action Cas Number
L-NIO dihydrochloride NOS inhibitor 159190-44-0

(1) ApexBio Compounds for NOS3 Gene

Compound Action Cas Number
1400W dihydrochloride INOS inhibitor,potent and highly selective 214358-33-5
genes like me logo Genes that share compounds with NOS3: view

Drug Products

Transcripts for NOS3 Gene

Unigene Clusters for NOS3 Gene

Nitric oxide synthase 3 (endothelial cell):
Representative Sequences:

Alternative Splicing Database (ASD) splice patterns (SP) for NOS3 Gene

No ASD Table

Relevant External Links for NOS3 Gene

GeneLoc Exon Structure for
NOS3
ECgene alternative splicing isoforms for
NOS3

Expression for NOS3 Gene

mRNA expression in normal human tissues for NOS3 Gene

mRNA differential expression in normal tissues according to GTEx for NOS3 Gene

This gene is overexpressed in Spleen (x8.7).

Protein differential expression in normal tissues from HIPED for NOS3 Gene

This gene is overexpressed in Placenta (22.1), Spleen (17.2), and Uterus (6.5).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, and MOPED for NOS3 Gene



SOURCE GeneReport for Unigene cluster for NOS3 Gene Hs.647092

mRNA Expression by UniProt/SwissProt for NOS3 Gene

P29474-NOS3_HUMAN
Tissue specificity: Platelets, placenta, liver and kidney.
genes like me logo Genes that share expression patterns with NOS3: view

Primer Products

In Situ Assay Products

No data available for Protein tissue co-expression partners for NOS3 Gene

Orthologs for NOS3 Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for NOS3 Gene

Organism Taxonomy Gene Similarity Type Details
cow
(Bos Taurus)
Mammalia NOS3 35
  • 90.67 (n)
  • 94.72 (a)
NOS3 36
  • 94 (a)
OneToOne
dog
(Canis familiaris)
Mammalia NOS3 35
  • 90.41 (n)
  • 94.93 (a)
NOS3 36
  • 95 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Nos3 35
  • 88.46 (n)
  • 94.73 (a)
Nos3 16
Nos3 36
  • 94 (a)
OneToOne
chimpanzee
(Pan troglodytes)
Mammalia NOS3 35
  • 99.56 (n)
  • 99.83 (a)
NOS3 36
  • 100 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Nos3 35
  • 87.94 (n)
  • 94.65 (a)
oppossum
(Monodelphis domestica)
Mammalia NOS3 36
  • 91 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia NOS3 36
  • 82 (a)
OneToOne
chicken
(Gallus gallus)
Aves INOS 36
  • 49 (a)
ManyToMany
lizard
(Anolis carolinensis)
Reptilia NOS3 36
  • 71 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia nos3 35
  • 66.96 (n)
  • 67.8 (a)
fruit fly
(Drosophila melanogaster)
Insecta Nos 37
  • 47 (a)
Nos 36
  • 41 (a)
OneToMany
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes NCP1 36
  • 25 (a)
OneToMany
sea squirt
(Ciona savignyi)
Ascidiacea CSA.5751 36
  • 49 (a)
OneToMany
Species with no ortholog for NOS3:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)
  • zebrafish (Danio rerio)

Evolution for NOS3 Gene

ENSEMBL:
Gene Tree for NOS3 (if available)
TreeFam:
Gene Tree for NOS3 (if available)

Paralogs for NOS3 Gene

Paralogs for NOS3 Gene

(3) SIMAP similar genes for NOS3 Gene using alignment to 4 proteins:

genes like me logo Genes that share paralogs with NOS3: view

Variants for NOS3 Gene

Sequence variations from dbSNP and Humsavar for NOS3 Gene

SNP ID Clin Chr 07 pos Sequence Context AA Info Type
rs1799983 - 150,999,023(+) GATGA(G/T)CCCCC reference, missense
rs3918166 - 150,996,468(+) GGGCC(A/C/G)GCCCT reference, missense
rs3918232 - 151,009,552(+) AGCCC(A/G)TGGCA reference, missense
rs3918201 - 151,010,256(+) GCCCA(G/T)GGAAC reference, missense
rs3918234 - 151,010,947(+) AAGCC(A/T)GCTCA reference, missense

Structural Variations from Database of Genomic Variants (DGV) for NOS3 Gene

Variant ID Type Subtype PubMed ID
dgv7543n71 CNV Loss 21882294
dgv7544n71 CNV Loss 21882294
nsv889465 CNV Loss 21882294
nsv889469 CNV Gain 21882294
nsv889470 CNV Loss 21882294
esv2735394 CNV Deletion 23290073
nsv889471 CNV Loss 21882294
esv274999 CNV Loss 21479260
nsv889472 CNV Loss 21882294
nsv889473 CNV Loss 21882294
nsv889474 CNV Loss 21882294

Variation tolerance for NOS3 Gene

Residual Variation Intolerance Score: 22.5% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 4.04; 60.52% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for NOS3 Gene

HapMap Linkage Disequilibrium report
NOS3
Human Gene Mutation Database (HGMD)
NOS3

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for NOS3 Gene

Disorders for NOS3 Gene

MalaCards: The human disease database

(67) MalaCards diseases for NOS3 Gene - From: OMIM, DISEASES, Novoseek, and GeneCards

Disorder Aliases PubMed IDs
preeclampsia/eclampsia 1
  • preeclampsia
stroke, ischemic
  • ischemic stroke
alzheimer disease
  • alzheimer disease, late-onset
hypertension, essential
  • hypertension, essential 3
acute chest syndrome
  • acute chest syndrome in sickle cell disease
- elite association - COSMIC cancer census association via MalaCards
Search NOS3 in MalaCards View complete list of genes associated with diseases

UniProtKB/Swiss-Prot

NOS3_HUMAN
  • Note=Variation in NOS3 seem to be associated with susceptibility to coronary spasm. {ECO:0000269 PubMed:11740345, ECO:0000269 PubMed:9737779}.

Relevant External Links for NOS3

Genetic Association Database (GAD)
NOS3
Human Genome Epidemiology (HuGE) Navigator
NOS3
Atlas of Genetics and Cytogenetics in Oncology and Haematology:
NOS3
genes like me logo Genes that share disorders with NOS3: view

No data available for Genatlas for NOS3 Gene

Publications for NOS3 Gene

  1. In-vivo effects of Glu298Asp endothelial nitric oxide synthase polymorphism. (PMID: 11740345) Sofowora G. … Wood A.J. (Pharmacogenetics 2001) 3 4 48 67
  2. Human vascular smooth muscle cells express a constitutive nitric oxide synthase that insulin rapidly activates, thus increasing guanosine 3':5'-cyclic monophosphate and adenosine 3':5'-cyclic monophosphate concentrations. (PMID: 10440125) Trovati M. … Ghigo D. (Diabetologia 1999) 23 25 26
  3. Estrogen receptor-alpha gene transfer into bovine aortic endothelial cells induces eNOS gene expression and inhibits cell migration. (PMID: 10690351) Tan E. … Bhalla R.C. (Cardiovasc. Res. 1999) 23 25 26
  4. Atrial expression of endothelial nitric oxide synthase in patients with and without atrial fibrillation. (PMID: 19211271) Bukowska A. … Goette A. (Cardiovasc. Pathol. 2010) 3 23
  5. Low folate levels may be an atherogenic factor regardless of homocysteine levels in young healthy nonsmokers. (PMID: 19913857) Imamura A. … Okumura K. (Metab. Clin. Exp. 2010) 3 23

Products for NOS3 Gene

  • Addgene plasmids for NOS3

Sources for NOS3 Gene

Back to Top

Content