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NOS2 Gene

protein-coding   GIFtS: 67
GCID: GC17M026083

Nitric Oxide Synthase 2, Inducible

(Previous names: nitric oxide synthase 2A (inducible, hepatocytes))
(Previous symbol: NOS2A)
Microbiology & Infectious Diseases Congress
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(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
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Aliases
Nitric Oxide Synthase 2, Inducible1 2     NOS Type II2 3
NOS2A1 2 3 5     EC 1.14.13.393 8
Nitric Oxide Synthase 2A (Inducible, Hepatocytes)1 2     INOS2
Hepatocyte NOS2 3     NOS2
Inducible NO Synthase2 3     Nitric Oxide Synthase, Inducible2
Inducible NOS2 3     Nitric Oxide Synthase, Macrophage2
Peptidyl-Cysteine S-Nitrosylase NOS22 3     NOS, Type II2
HEP-NOS2 3     iNOS3

External Ids:    HGNC: 78731   Entrez Gene: 48432   Ensembl: ENSG000000071717   OMIM: 1637305   UniProtKB: P352283   

Export aliases for NOS2 gene to outside databases

Previous GC identifers: GC17M023108 GC17M022290


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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Entrez Gene summary for NOS2 Gene:
Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including
neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which
is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three
related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17. (provided by RefSeq,
Jul 2008)

GeneCards Summary for NOS2 Gene:
NOS2 (nitric oxide synthase 2, inducible) is a protein-coding gene. Diseases associated with NOS2 include malaria, and virilizing ovarian tumor. GO annotations related to this gene include calmodulin binding and protein homodimerization activity. An important paralog of this gene is NOS3.

UniProtKB/Swiss-Prot: NOS2_HUMAN, P35228
Function: Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In
macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates
cysteine S-nitrosylation of cytoplasmic target proteins such COX2

summary for NOS2 Gene:
Inducible nitric oxide synthase (iNOS), along with neuronal nitric oxide synthase (nNOS) and endothelial
nitric oxide synthase (eNOS), catalyze the generation of nitric oxide and L-citrulline from L-arginine and
molecular oxygen. It displays high affinity for Ca2+/calmodulin, thus appears to be constitutively active.
Tetrahydrobiopterin, flavin adenine dinucleotide, flavin mononucleotide and NADPH co-factors are required
for catalytic activity. iNOS gene expression is regulated by various pathways including NF-kappaB, IFN-gamma
(signaling through the JAK-STAT cascade) and HIF-1, which induce expression, and TGF-beta and interleukins
-4, -10 and -13, which inhibit expression. Post-translational regulation occurs by prevention of iNOS
homodimerization by NAP110. iNOS has roles in the innate immune system and displays antibacterial, antiviral
and antifungal activity. The human gene encoding iNOS is localized to chromosome 17q11.2-q12.

Gene Wiki entry for NOS2 (Nitric oxide synthase 2) Gene


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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Regulatory elements:
   Regulatory transcription factor binding sites in the NOS2 gene promoter:
         p300   MyoD   IRF-1   HNF-3beta   HNF-4alpha1   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmids (see all 3): NOS2 promoter sequence
   Search Chromatin IP Primers for NOS2

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat NOS2


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 17q11.2-q12   Ensembl cytogenetic band:  17q11.2   HGNC cytogenetic band: 17q11.2-q12

NOS2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
NOS2 gene location

GeneLoc information about chromosome 17         GeneLoc Exon Structure

GeneLoc location for GC17M026083:  view genomic region     (about GC identifiers)

Start:
26,083,792 bp from pter      End:
26,127,555 bp from pter
Size:
43,764 bases      Orientation:
minus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Cloud-Clone Corp.,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Cloud-Clone Corp., Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, and/or Cloud-Clone Corp.)
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UniProtKB/Swiss-Prot: NOS2_HUMAN, P35228 (See protein sequence)
Recommended Name: Nitric oxide synthase, inducible  
Size: 1153 amino acids; 131117 Da
Cofactor: Heme group (By similarity)
Cofactor: Binds 1 FAD (By similarity)
Cofactor: Binds 1 FMN (By similarity)
Cofactor: Tetrahydrobiopterin (BH4). May stabilize the dimeric form of the enzyme (By similarity)
Subunit: Homodimer. Binds SLC9A3R1
Selected PDB 3D structures from and Proteopedia for NOS2 (see all 7):
1NSI (3D)        2LL6 (3D)        2NSI (3D)        3E7G (3D)        3EJ8 (3D)        3HR4 (3D)    
Secondary accessions: A1L3U5 B7ZLY2 O60757 O94994 Q16263 Q16692 Q4TTS5 Q9UD42
Alternative splicing: 2 isoforms:  P35228-1   P35228-2   

Explore the universe of human proteins at neXtProt for NOS2: NX_P35228

Explore proteomics data for NOS2 at MOPED

Post-translational modifications: 

  • Modification sites at neXtProt
  • Modification sites at PhosphoSitePlus
  • Selected DME Specific Peptides for NOS2 (P35228) (see all 43)
     VLEEFPS  GWYMGTE  LDITTPP  LQVFDAR 


    See NOS2 Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins: NP_000616.3  
    ENSEMBL proteins: 
     ENSP00000327251  
    Reactome Protein details: P35228

    NOS2 Human Recombinant Protein Products:

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    OriGene Purified Protein for NOS2
    OriGene Protein Over-expression Lysate for NOS2
    OriGene MassSpec for NOS2
    OriGene Custom Protein Services for NOS2
    GenScript Custom Purified and Recombinant Proteins Services for NOS2
    Novus Biologicals NOS2 Protein
    Novus Biologicals NOS2 Lysate
    Browse Sino Biological Recombinant Proteins
    Browse Sino Biological Cell Lysates
    Browse ProSpec Recombinant Proteins
    Cloud-Clone Corp. Proteins for NOS2

    NOS2 Antibody Products:

    EMD Millipore Mono- and Polyclonal Antibodies for the study of NOS2
    R&D Systems Antibodies for NOS2 (iNOS)
    OriGene Antibodies for NOS2
    OriGene Custom Antibody Services for NOS2
    Novus Biologicals NOS2 Antibodies
    Abcam antibodies for NOS2 (P29475, P35228)
    Cloud-Clone Corp. Antibodies for NOS2
    ThermoFisher Antibody for NOS2
    LSBio Antibodies in human, mouse, rat for NOS2

    NOS2 Assay Products:

    Browse Kits and Assays available from EMD Millipore
    OriGene Custom Assay Services for NOS2
    R&D Systems ELISAs for NOS2 (iNOS)
    GenScript Custom Assay Services for NOS2
    Browse Enzo Life Sciences for kits & assays
    Cloud-Clone Corp. ELISAs for NOS2
    Cloud-Clone Corp. CLIAs for NOS2


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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    IUPHAR Guide to PHARMACOLOGY protein family classification: Inducible NOS
    Nitric oxide synthases

    Selected InterPro protein domains (see all 10):
     IPR017927 Fd_Rdtase_FAD-bd
     IPR001094 Flavdoxin
     IPR008254 Flavodoxin/NO_synth
     IPR012144 NOS_euk
     IPR004030 NO_synthase_oxygenase_dom

    Graphical View of Domain Structure for InterPro Entry P35228

    ProtoNet protein and cluster: P35228

    Selected Blocks protein domains (see all 6):
    IPB001094 Flavodoxin signature
    IPB001433 Oxidoreductase FAD/NAD(P)-binding
    IPB001709 Flavoprotein pyridine nucleotide cytochrome reductase signature
    IPB003097 FAD-binding
    IPB004030 Nitric oxide synthase


    UniProtKB/Swiss-Prot: NOS2_HUMAN, P35228
    Similarity: Belongs to the NOS family
    Similarity: Contains 1 FAD-binding FR-type domain
    Similarity: Contains 1 flavodoxin-like domain


    NOS2 for domains           About GeneDecksing


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Molecular Function:

         UniProtKB/Swiss-Prot Summary: NOS2_HUMAN, P35228
    Function: Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In
    macrophages, NO mediates tumoricidal and bactericidal actions. Also has nitrosylase activity and mediates
    cysteine S-nitrosylation of cytoplasmic target proteins such COX2
    Catalytic activity: 2 L-arginine + 3 NADPH + 4 O(2) = 2 L-citrulline + 2 nitric oxide + 3 NADP(+) + 4 H(2)O
    Enzyme regulation: Regulated by calcium/calmodulin. Aspirin inhibits expression and function of this enzyme and
    effects may be exerted at the level of translational/post-translational modification and directly on the
    catalytic activity (By similarity)
    Induction: By endotoxins and cytokines. Induced by IFNG/IFN-gamma acting synergistically with bacterial
    lipopolysaccharides (LPS), TNF or IL1B/interleukin-1 beta

         Enzyme Number (IUBMB): EC 1.14.13.391 2

         Gene Ontology (GO): Selected molecular function terms (see all 14):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003958NADPH-hemoprotein reductase activity IBA--
    GO:0004517nitric-oxide synthase activity IDA7504305
    GO:0005102receptor binding IPI--
    GO:0005506iron ion binding IEA--
    GO:0005515protein binding IPI12855682
         
    NOS2 for ontologies           About GeneDecksing


    Phenotypes:
         2 GenomeRNAi human phenotypes for NOS2:
     Decreased viability after Chla  Increased gamma-H2AX phosphory 

         Selected MGI mutant phenotypes (inferred from 3 alleles(MGI details for Nos2) (see all 23):
     adipose tissue  behavior/neurological  cardiovascular system  cellular  craniofacial 
     digestive/alimentary  endocrine/exocrine gland  growth/size/body  hematopoietic system  homeostasis/metabolism 
     immune system  integument  liver/biliary system  mortality/aging  muscle 

    NOS2 for phenotypes           About GeneDecksing

    Animal Models:
         MGI mouse knock-outs for NOS2: Nos2tm1Mrl Nos2tm1Lau Nos2tm1Liew

       inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for NOS2
       inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for NOS2

       genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for NOS2
       genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for NOS2

    miRNA
    Products:
        
    miRTarBase miRNAs that target NOS2:
    hsa-mir-26a-5p (MIRT007347)

    Block miRNA regulation of human, mouse, rat NOS2 using miScript Target Protectors
    2 qRT-PCR Assays for microRNAs that regulate NOS2:
    hsa-miR-485-3p hsa-miR-323-5p
    SwitchGear 3'UTR luciferase reporter plasmidNOS2 3' UTR sequence
    Inhib. RNA
    Products:
        
    OriGene RNAi products in human, mouse, rat for NOS2
    Predesigned siRNA for gene silencing in human, mouse, rat NOS2

    Gene Editing
    Products:
    DNA2.0 Custom Protein Engineering Service for NOS2

    Clone
    Products:
         
    OriGene clones in human, mouse for NOS2 (see all 6)
    OriGene ORF clones in mouse, rat for NOS2
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector: NOS2 (NM_000625)
    Browse Sino Biological Human cDNA Clones
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for NOS2
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat NOS2

    Cell Line
    Products:
         
    GenScript Custom overexpressing Cell Line Services for NOS2
    Browse ESI BIO Cell Lines and PureStem Progenitors for NOS2 
    In Situ Assay
    Products:
       

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for NOS2


    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    peroxisome5
    cytosol3
    nucleus3
    cytoskeleton2
    extracellular2
    mitochondrion2
    plasma membrane2
    endoplasmic reticulum1
    lysosome1
    vacuole1

    Gene Ontology (GO): Selected cellular component terms (see all 7):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005622intracellular IDA7544003
    GO:0005634nucleus ISS--
    GO:0005737cytoplasm IDA12085352
    GO:0005777peroxisome IDA12085352
    GO:0005829cytosol TAS--

    NOS2 for ontologies           About GeneDecksing


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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    SuperPaths for NOS2 About   (see all 37)  
    See pathways by source

    SuperPathContained pathways About
    1Arginine and proline metabolism
    Arginine and proline metabolism
    citrulline-nitric oxide cycle0.00
    superpathway of citrulline metabolism0.00
    2IL12-mediated signaling events
    Immune response IL 12 induced IFN gamma production0.33
    IL12-mediated signaling events0.33
    3Immune response Bacterial infections in normal airways
    Immune response Bacterial infections in normal airways0.78
    Bacterial infections in CF airways0.78
    4Leishmaniasis
    Leishmaniasis0.35
    Toxoplasmosis0.35
    5Immune response CCR3 signaling in eosinophils
    Immune response CCR3 signaling in eosinophils0.51

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways

    Selected Downloadable PowerPoint Slides of GeneGlobe Pathway Central Maps for NOS2 (see all 10)
        MIF Regulation of Innate Immune Cells
    MSP-RON Signaling
    Sertoli-Sertoli Cell Junction Dynamics
    Endothelin-1 Signaling Pathway
    Relaxin Pathway

    Selected GeneGo (Thomson Reuters) Pathways for NOS2 (see all 11)
        Immune response MIF-mediated glucocorticoid regulation
    Immune response Bacterial infections in normal airways
    Immune response MIF in innate immunity response
    Development VEGF signaling via VEGFR2 - generic cascades
    Mechanisms of CFTR activation by S-nitrosoglutathione (normal and CF)

    Selected BioSystems Pathways for NOS2 (see all 12)
        Type II interferon signaling (IFNG)
    AGE/RAGE pathway
    Spinal Cord Injury
    Effects of nitric oxide
    citrulline-nitric oxide cycle

    2 Reactome Pathways for NOS2
        Phagosomal maturation (early endosomal stage)
    Nitric oxide stimulates guanylate cyclase

    2 PharmGKB Pathways for NOS2
        Doxorubicin Pathway (Cardiomyocyte Cell), Pharmacodynamics
    Doxorubicin Pathway, Pharmacokinetics

    Selected Kegg Pathways  (Kegg details for NOS2) (see all 14):
        Arginine and proline metabolism
    Metabolic pathways
    Calcium signaling pathway
    HIF-1 signaling pathway
    Peroxisome


    NOS2 for pathways           About GeneDecksing

        Pathway & Disease-focused RT2 Profiler PCR Arrays including NOS2 (see all 14): 
              Insulin Signaling Pathway in human mouse rat
              G-Protein-Coupled Receptor Signaling PathwayFinder in human mouse rat
              Oxidative Stress in human mouse rat
              Nitric Oxide Signaling Pathway in human mouse rat
              Skeletal Muscle: Myogenesis & Myopathy in human mouse rat

    Interactions:

        GeneGlobe Interaction Network for NOS2

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    Selected Interacting proteins for NOS2 (P352283 ENSP000003272514) via UniProtKB, MINT, STRING, and/or I2D (see all 82)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    CALM2P621583, ENSP000002722984I2D: score=1 STRING: ENSP00000272298
    CALM1P621583, ENSP000003494674I2D: score=1 STRING: ENSP00000349467
    CALM3P621583I2D: score=1 
    ACTN4O437073, ENSP000002526994I2D: score=4 STRING: ENSP00000252699
    RAC2P151533, ENSP000002490714I2D: score=4 STRING: ENSP00000249071
    About this table

    Gene Ontology (GO): Selected biological process terms (see all 27):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0001666response to hypoxia IEA--
    GO:0001912positive regulation of leukocyte mediated cytotoxicity TAS10450191
    GO:0002227innate immune response in mucosa NAS18390757
    GO:0006527arginine catabolic process IDA7504305
    GO:0006801superoxide metabolic process ISS--

    NOS2 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
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    Browse Small Molecules at EMD Millipore
       Enzo Life Sciences drugs & compounds for NOS2
      Browse compounds at ApexBio 

    Compounds for NOS2 available from Tocris Bioscience    About this table
    CompoundAction CAS #
    BYK 191023 dihydrochloridePotent and selective inhibitor of iNOS[608880-48-4]
    L-NIL hydrochlorideSelective iNOS inhibitor[150403-89-7]
    1400W dihydrochloride Potent, highly selective iNOS inhibitor [180001-34-7]
    AMT hydrochloride Potent, selective iNOS inhibitor [21463-31-0]
    (S)-Methylisothiourea sulfateHighly selective iNOS inhibitor[867-44-7]

    10 HMDB Compounds for NOS2    About this table
    CompoundSynonyms CAS #PubMed Ids
    CalciumCa (see all 2)7440-70-2--
    Citrulline(2S)-2-amino-5-(carbamoylamino)pentanoic acid (see all 55)372-75-8--
    FAD1H-Purin-6-amine flavin dinucleotide (see all 21)146-14-5--
    Flavin MononucleotideFMN (see all 19)146-17-8--
    Heme(protoporphyrinato)iron (see all 19)14875-96-8--
    L-Arginine(S)-2-Amino-5-[(aminoiminomethyl)amino]pentanoate (see all 14)74-79-3--
    NADPAdenine-nicotinamide dinucleotide phosphate (see all 18)53-59-8--
    NADPH2'-(dihydrogen phosphate) 5'-(trihydrogen pyrophosphate) Adenosine 5'-ester with 1,4-dihydro-1-b-D-ribofuranosylnicotinamide (see all 23)53-57-6--
    Nitric oxideMononitrogen monoxide (see all 9)10102-43-9--
    Tetrahydrobiopterin(1R,2S)-(2-Amino-3,4,5,6,7,8-hexahydro-4-oxo-6-pteridinyl)-1,2-propandiol (see all 11)17528-72-2--

    Selected DrugBank Compounds for NOS2 (see all 40)    About this table
    CompoundSynonyms CAS #TypeActionsPubMed Ids
    L-Arginine(S)-2-Amino-5-guanidinovaleric acid (see all 4)74-79-3target--17513447 17340133 16778961 17362916 17363779
    L-Citrulline2-Amino-5-uredovaleric acid (see all 8)372-75-8target--12745988 10906994 11742852 10906995 10944418
    Aminoguanidine-- 79-17-4targetinhibitor17139284 11752352 17016423 10592235
    DexamethasoneDesametasone (see all 9)50-02-2targetnegative modulator17494951 17503181 11752352 17379310
    Miconazole-- 22916-47-8targetinhibitor7536941 7518297 7683652 9873034
    Triflusal-- --targetagonist9551715 19465361 16939630 10998082
    3-Bromo-7-Nitroindazole-- --target--17139284 17016423 10592235
    4r-Fluoro-N6-Ethanimidoyl-L-Lysine-- --target--17139284 17016423 10592235
    5-Nitroindazole-- --target--17139284 17016423 10592235
    6-Nitroindazole-- --target--17139284 17016423 10592235

    5 IUPHAR Ligands for NOS2 (Inducible NOS)    About this table
    LigandTypeActionAffinityPubmed IDs
    1400W
    InhibitorInhibition8.29030556
    2-amino-4-methylpyridine
    InhibitorInhibition7.48937711
    PIBTU
    InhibitorInhibition7.37523409
    NIL
    InhibitorInhibition5.57525961
    aminoguanidine
    InhibitorInhibition5.51378415

    Selected Novoseek inferred chemical compound relationships for NOS2 gene (see all 124)    About this table
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    nitric oxide 97.5 18892 11680730 (10), 11342896 (8), 16957516 (8), 18554637 (8) (see all 99)
    citrulline 87.5 279 20148434 (3), 20148433 (3), 8561501 (3), 7527657 (2) (see all 99)
    l-nmma 87.3 488 9503327 (4), 12810424 (3), 17724209 (3), 18090547 (3) (see all 99)
    arginine 86.4 1048 16245240 (5), 11275358 (5), 8639550 (5), 7664438 (4) (see all 99)
    peroxynitrite 86.2 233 11788351 (4), 9180331 (3), 10383909 (3), 9122229 (3) (see all 99)
    aminoguanidine 84.9 363 9538001 (5), 16553284 (4), 18790185 (4), 11062159 (3) (see all 99)
    adma 83.5 234 8743168 (5), 15325021 (4), 19682580 (4), 10802528 (3) (see all 99)
    tetrahydrobiopterin 79.4 94 15565652 (2), 14609340 (2), 16052472 (2), 16172396 (2) (see all 67)
    cgmp 78.8 303 18606420 (5), 10802528 (4), 11181408 (4), 8633049 (4) (see all 99)
    n(g)-nitro-l-arginine methyl ester 76.8 176 11891189 (2), 7684763 (1), 7673269 (1), 8991992 (1) (see all 99)



    NOS2 for compounds           About GeneDecksing



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    Conferences by KenesGroup, exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, and/or QIAGEN )
    About This Section

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    REFSEQ mRNAs for NOS2 gene (2 alternative transcripts): 
    NM_000625.4  NM_153292.1  

    1 Ensembl transcript including schematic representation, and UCSC links where relevant:
    ENST00000313735(uc002gzu.3 uc010wab.1 uc010crh.1)
    Congresses - knowledge worth sharing:
    European Congress of Clinical Microbiology and Infectious Diseases (ECCMID) 10 - 13 May 2014

    miRNA
    Products:
         
    Block miRNA regulation of human, mouse, rat NOS2 using miScript Target Protectors
    2 qRT-PCR Assays for microRNAs that regulate NOS2:
    hsa-miR-485-3p hsa-miR-323-5p
    SwitchGear 3'UTR luciferase reporter plasmidNOS2 3' UTR sequence
    Inhib. RNA
    Products:
         
    OriGene RNAi products in human, mouse, rat for NOS2
    Predesigned siRNA for gene silencing in human, mouse, rat NOS2
    Clone
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    OriGene clones in human, mouse for NOS2 (see all 6)
    OriGene ORF clones in mouse, rat for NOS2
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector: NOS2 (NM_000625)
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for NOS2
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat NOS2
    Primer
    Products:
        
    OriGene qSTAR qPCR primer pairs in human, mouse for NOS2
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat NOS2
      QuantiTect SYBR Green Assays in human, mouse, rat NOS2
      QuantiFast Probe-based Assays in human, mouse, rat NOS2

    Selected AceView cDNA sequences (see all 75):

    NM_153292 AW445097 S75615 S76479 BM834810 AW297448 BE073577 NM_000625 
    X73029 BE073638 AI797675 AW449618 AI953734 AI738789 CD609259 AI991263 
    L24553 U31511 CD609257 BV207664 BV188741 AA877840 CD609248 H67843 

    GeneLoc Exon Structure

    3 Alternative Splicing Database (ASD) splice patterns (SP) for NOS2    About this scheme

    ExUns: 1 ^ 2a · 2b ^ 3 ^ 4 ^ 5 ^ 6 ^ 7 ^ 8 ^ 9 ^ 10 ^ 11 ^ 12 ^ 13 ^ 14 ^ 15 ^ 16 ^ 17 ^ 18 ^ 19 ^ 20 ^ 21 ^ 22 ^ 23 ^ 24 ^ 25 ^
    SP1:                    -                                                                                                                                       
    SP2:                    -                                                                                                                                       
    SP3:                                      -                                                                                                                     

    ExUns: 26 ^ 27 ^ 28
    SP1:                  
    SP2:                  
    SP3:                  


    ECgene alternative splicing isoforms for NOS2

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

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    NOS2 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: AATTGCATTT
    NOS2 Expression
    About this image


    NOS2 expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database
     selected tissues (see all 6) fully expand
     
     Bone (Muscoskeletal System)
             HyStem+BMP4-induced 4D20.8 cells
     
     Neural Ectoderm (Nervous System)
             Neural ectoderm cells
     
     Cartilage (Muscoskeletal System)
             HyStem+BMP4-induced 4D20.8 cells
     
     Neural Tube (Nervous System)
             Floor plate-like cells
     
     Brain (Nervous System)
             N2/LSB induced-cells
    NOS2 Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    NOS2 Protein Expression

    UniProtKB/Swiss-Prot: NOS2_HUMAN, P35228
    Tissue specificity: Expressed in the liver, retina, bone cells and airway epithelial cells of the lung. Not
    expressed in the platelets

        Pathway & Disease-focused RT2 Profiler PCR Arrays including NOS2 (see all 14): 
              Insulin Signaling Pathway in human mouse rat
              G-Protein-Coupled Receptor Signaling PathwayFinder in human mouse rat
              Oxidative Stress in human mouse rat
              Nitric Oxide Signaling Pathway in human mouse rat
              Skeletal Muscle: Myogenesis & Myopathy in human mouse rat

    Primer
    Products:
    OriGene qSTAR qPCR primer pairs in human, mouse for NOS2
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat NOS2
    QuantiTect SYBR Green Assays in human, mouse, rat NOS2
    QuantiFast Probe-based Assays in human, mouse, rat NOS2
    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for NOS2

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

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    This gene was present in the common ancestor of animals and fungi.

    Orthologs for NOS2 gene from Selected species (see all 15)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Nos21 , 5 nitric oxide synthase 2, inducible1, 5 82.4(n)1
    81.21(a)1
      11 (46.74 cM)5
    181261  NM_010927.31  NP_035057.11 
     789207875 
    chicken
    (Gallus gallus)
    Aves NOS21 nitric oxide synthase 2, inducible 68.43(n)
    70.46(a)
      395807  NM_204961.1  NP_990292.1 
    lizard
    (Anolis carolinensis)
    Reptilia NOS26
    nitric oxide synthase 2, inducible
    56(a)
    1 ↔ 1
    GL343729.1(116369-178014)
    tropical clawed frog
    (Xenopus tropicalis)
    Amphibia nos21 nitric oxide synthase 2, inducible 65.96(n)
    65.96(a)
      100494698  XM_002935296.2  XP_002935342.2 
    zebrafish
    (Danio rerio)
    Actinopterygii nos2b1 nitric oxide synthase 2b, inducible 61.56(n)
    60.63(a)
      563654  NM_001113501.1  NP_001106973.1 
    fruit fly
    (Drosophila melanogaster)
    Insecta Nos6
    Nitric oxide synthase
    38(a)
    1 → many
    2L(10804269-10838448)
    baker's yeast
    (Saccharomyces cerevisiae)
    Saccharomycetes NCP16
    NADP-cytochrome P450 reductase; involved in ergost...
    24(a)
    1 → many
    VIII(190543-192618) YHR042W


    ENSEMBL Gene Tree for NOS2 (if available)
    TreeFam Gene Tree for NOS2 (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section

    TryGeneCards Plus
    Paralogs for NOS2 gene
    NOS32  POR2  MTRR2  NOS12  
    2 SIMAP similar genes for NOS2 using alignment to 1 protein entry:     NOS2_HUMAN:
    NOS1    NOS3

    NOS2 for paralogs           About GeneDecksing


    Selected Pseudogenes.org Pseudogenes for NOS2 (see all 15)
    PGOHUM00000247699 PGOHUM00000248028 PGOHUM00000250807 PGOHUM00000237000 PGOHUM00000237005


    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
    About This Section

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    Polymorphic Variants from UniProtKB/Swiss-Prot
    NOS2_HUMAN, P35228: Note=Genetic variations in NOS2 are involved in resistance to malaria [MIM:611162]


    Selected SNPs for NOS2 (see all 1440)    About this table    
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 17 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    VAR_0363024
    A breast cancer sample4--see VAR_0363022 A S mis40--------
    rs596857461,2
    C--26088338(+) CACGG-/CCCTCC 1 -- int13Minor allele frequency- C:0.00NA CSA 8
    rs58198211,2
    C,F--26106756(+) CAGAAA/-GCTGG 1 -- int17Minor allele frequency- -:0.31NS NA CSA 178
    rs1123369321,2
    C--26115222(+) GCGTGCACA/-  
            
    CACAC
    1 -- int11Minor allele frequency- -:0.50CSA 2
    rs594313571,2
    C,F--26115249(+) CACAC-/AC/ACAC/ACACAC
    ACAC
    /ACACACACACAC
    GAATG
    2 -- int1 cds12NA 4
    rs349927771,2
    C--26119841(+) CTACAA/-AAAAA 1 -- int11Minor allele frequency- -:0.00NA 2
    rs289987991,2
    C,F--26126131(-) GGGGGG/-TTCAT 1 -- int16Minor allele frequency- -:0.07NS NA 184
    rs358348121,2
    C--26126817(+) AAAAA-/AGAAAG 1 -- int11Minor allele frequency- A:0.50NA 2
    rs1462260231,2
    --26143243(+) TCCTTA/GGCCTG 1 -- ds50010--------
    rs1154286341,2
    F--26143250(+) CCTGCT/ACCAAA 1 -- ds50011Minor allele frequency- A:0.01WA 118
    rs289442221,2
    C,F--26143256(-) CTTGGG/ATTTGG 1 -- ds50015Minor allele frequency- A:0.09NS WA 244
    rs124505211,2
    C,F,A,H--26143302(+) CCACAC/ATCGCC 1 -- ds500130Minor allele frequency- A:0.23NS EA NA WA 3272
    rs289442211,2
    C,F--26143310(-) CGTGTC/TGAGGC 1 -- ds50016Minor allele frequency- T:0.07NS WA 246
    rs289442201,2
    C,F--26143433(-) TGTTTC/TGAGGA 1 -- ds50015Minor allele frequency- T:0.06NS WA 244
    rs289442191,2
    C--26143438(-) GCCAGT/CGTTTC 1 -- ds50014Minor allele frequency- C:0.00NS 126
    rs74066571,2
    C,F,A,H--26143600(+) GACTGG/CCTGGG 1 -- ds500116Minor allele frequency- C:0.18NS EA NA CSA WA 1336
    rs289442181,2
    C,F--26143614(-) CGACAG/AAGGGA 1 -- ds50016Minor allele frequency- A:0.06NS WA 256
    rs1391970401,2
    C--26143661(+) ACTCTC/GAGAAG 1 -- ds50010--------
    rs1887480171,2
    --26143764(+) AAGACC/TCAAGT 1 -- ut310--------
    rs289442171,2
    C--26143767(-) GCCACT/CTGGGT 1 -- ut31 ese34Minor allele frequency- C:0.01NS 138
    rs1440040511,2
    C--26143829(+) TGTACA/GCAAGG 1 -- ut310--------
    rs289442161,2
    C--26143855(-) CTATCG/ACACCA 1 -- ut31 ese37Minor allele frequency- A:0.01NS NA 572
    rs289442151,2
    C--26143858(-) GTGCTA/GTCGCA 1 -- ut315Minor allele frequency- G:0.01NS 568
    rs289442141,2
    C,F,H--26143906(-) AAGATG/AGAACT 1 -- ut3118Minor allele frequency- A:0.01NS EA NA 2162
    rs157821,2
    --26143945(-) GAGACA/GAAATC 1 -- ut31 ese32Minor allele frequency- G:0.31NA MN 186
    rs1916024721,2
    C--26143991(+) GCTCCA/GATCAA 1 -- ut310--------
    rs1848431871,2
    --26144065(+) AAGACG/TTGAGG 1 -- ut310--------
    rs32017421,2
    C--26144116(-) TTATCT/CGAGGT 1 -- ut31 ese33Minor allele frequency- C:0.47MN NA 188
    rs1999205491,2
    C--26144169(+) AACCCC/GTCCTG 1 -- ut310--------
    rs2009881161,2
    C--26144178(+) TGTAGA/GCCCTC 1 -- ut310--------
    rs1997485791,2
    --26144196(+) TGACAC/TCTCCA 2 M V mis10--------
    rs2007482261,2
    C--26144219(+) GCACCA/GCCACC 2 A V mis10--------
    rs72231101,2
    C,F--26144236(+) TTCTTC/TGCCTC 2 A syn11Minor allele frequency- T:0.00NA 4552
    rs2006327381,2
    C--26144237(+) TCTTCA/GCCTCG 2 A V mis10--------
    rs11379491,2
    C--26144272(-) TATCAC/TGAAGA 2 H syn12Minor allele frequency- T:0.00NA 4
    rs2001709471,2
    C--26144314(+) AGCAAC/TGTGTC 1 -- int10--------
    rs116537161,2
    C,F,H--26144442(+) CTAACC/GCTGCT 1 -- int115Minor allele frequency- G:0.14NS EA WA NA CSA 1450
    rs289442131,2
    C,F--26144466(-) GGGCCC/TGCACC 1 -- int16Minor allele frequency- T:0.00NS CSA 658
    rs289442121,2
    C,F--26144707(-) TCGAAT/CAGCCT 1 -- int14Minor allele frequency- C:0.01NS 138
    rs1817073891,2
    --26144792(+) CTGTTA/CTTCAA 1 -- int10--------
    rs1850591711,2
    --26144802(+) AACACA/GCCAGC 1 -- int10--------
    rs1902989501,2
    --26144812(+) CTTAAC/TTCCCA 1 -- int10--------
    rs289442111,2
    C,F--26144823(-) GGCCCC/TGGGGG 1 -- int15Minor allele frequency- T:0.11NS 688
    rs1817459561,2
    --26144839(+) ACCTGC/GTGCCC 1 -- int10--------
    rs80804181,2
    C,F,A,H--26144896(+) CCTCAC/TCATCT 1 -- int111Minor allele frequency- T:0.27NA NS WA 712
    rs1458159051,2
    --26144974(+) CCCAGC/TGTTCC 1 -- int10--------
    rs72087751,2
    C,F,H--26145018(+) CTACAC/GCTCTT 1 -- int1 trp322Minor allele frequency- G:0.09NS EA NA WA 2924
    rs1862627521,2
    --26145187(+) AACCAC/GAGAGA 1 -- int10--------
    rs289442101,2
    C--26145203(-) TAGAGT/CTCAGT 1 -- int14Minor allele frequency- C:0.01NS 136
    rs1455425541,2
    --26145213(+) AGGCTC/TTCACA 1 -- int10--------
    rs1896825381,2
    --26145226(+) TACTGC/TTGTGG 1 -- int10--------
    rs1809708391,2
    --26145268(+) GGGTGC/TCTCCC 1 -- int10--------
    rs289442091,2
    C,F--26145281(-) GAGGAC/ATTTAT 1 -- int14Minor allele frequency- A:0.02NS 138
    rs1424053571,2
    --26145293(+) GCAGGA/GCTGAA 1 -- int10--------
    rs289442081,2
    C,F--26145470(-) GCCCCC/TCGTCA 1 -- int14Minor allele frequency- T:0.02NS 144
    rs72136911,2
    C--26145536(+) AAAGGG/AGGGTT 1 -- int1 trp34Minor allele frequency- A:0.01NS 144
    rs289442071,2
    C,F--26145633(-) ATCCTG/AGACCA 1 -- int15Minor allele frequency- A:0.02NS CSA 147
    rs1910679001,2
    --26145646(+) GCAGTA/TAGGCC 1 -- int10--------
    rs65054451,2
    C,F,A,H--26145647(+) CAGTAG/AGGCCA 1 -- int115Minor allele frequency- A:0.37NS NA WA CSA 643
    rs1512389311,2
    C--26145705(+) TGGGGC/TCCAGA 1 -- int10--------
    rs2015204941,2
    C--26145767(+) TCCAAC/TGGCCT 1 -- int10--------
    rs2015550441,2
    C--26145785(+) TGGCCA/GGCACC 1 -- int10--------
    rs1995683971,2
    C--26145788(+) CCGGCA/TCCTTC 1 -- int10--------
    rs289442061,2
    F--26145792(-) GGCTGA/-AGGTG 1 -- int14Minor allele frequency- -:0.03NS 116
    rs2009762961,2
    C--26145797(+) TCAGCC/TCACAC 1 -- int10--------
    rs1833424911,2
    C--26145803(+) CACACA/TCCCAC 1 -- int10--------
    rs1996515571,2
    C--26145806(+) ACACCC/TACTAC 1 -- int10--------
    rs1882232361,2
    --26145837(+) ATAGTC/TCTCGA 2 N D mis10--------
    rs1417304581,2
    C,F--26145838(+) TAGTCC/TTCGAC 2 E syn11Minor allele frequency- T:0.00NA 4518
    rs37296621,2
    C,F--26145841(-) CAGGTC/TGAGGA 2 V syn13Minor allele frequency- T:0.01NA WA 4562
    rs1134194641,2
    C,F--26145915(+) CATGCG/ACACAT 2 /R /C mis12Minor allele frequency- A:0.00CSA NA 4530
    rs1390053031,2
    C--26145973(+) ACCTCA/C/GCTGGC 3 S R mis1 syn11NA 4314
    rs2019951831,2
    C--26145989(+) GCTGCC/TGCAGG 2 Q R mis10--------
    rs2021985211,2
    C--26146022(+) ACCAGA/GGGAAA 1 -- int10--------
    rs2002349021,2
    C--26146023(+) CCAGAA/GGAAAC 1 -- int10--------
    rs1833526701,2
    --26146093(+) GGAGGA/CGGGGA 1 -- int10--------
    rs287308311,2
    C--26146137(-) TCCTGA/GCTGGA 1 -- int10--------
    rs776247851,2
    C,F--26146176(+) GTGCAT/CGGATG 1 -- int16Minor allele frequency- C:0.19NA CSA WA EA 245
    rs1386399911,2
    C--26146313(+) AGGCAC/GTCCCC 1 -- int10--------
    rs289442051,2
    C,F--26146389(-) ATACCC/TTGCAG 1 -- int15Minor allele frequency- T:0.02NS 654
    rs1880457331,2
    --26146391(+) GCAGGA/GTATGG 1 -- int10--------
    rs37947561,2
    C,F,H--26146539(+) AAGGCC/TGAGAG 1 -- int125Minor allele frequency- T:0.14EA NA NS WA 4550
    rs289442021,2
    C,F--26146682(-) CTGCCC/TGGGGA 1 -- int14Minor allele frequency- T:0.01NS 144
    rs1913557961,2
    --26146759(+) ACCTAG/TGTTAG 1 -- int10--------
    rs1379580271,2
    C--26146803(+) TATGAA/GAGGGT 1 -- int10--------
    rs1844504951,2
    --26146909(+) ACCCAA/GGGCTC 1 -- int10--------
    rs413375471,2
    C,F--26146931(-) TTACAC/TACACA 1 -- int17Minor allele frequency- T:0.01NA NS EU 1961
    rs1424235841,2
    C,F--26146937(+) TGTAAA/C/TGCTTC 1 -- int11EU 1323
    rs2017695321,2
    C--26146947(+) CACCTC/GCTTTG 1 -- int10--------
    rs1500619371,2
    C--26147006(+) GCACCG/ACATGC 2 /A /V mis11Minor allele frequency- A:0.00NA 4548
    rs2016430301,2
    --26147010(+) CGCATA/GCAGCA 2 H Y mis10--------
    rs1453836831,2
    C,F--26147016(+) CAGCAC/GCCCCT 2 L V mis12Minor allele frequency- G:0.00NA EU 5869
    rs1492016851,2
    C,F--26147073(+) TGGGCG/TGCGGC 2 S R mis11Minor allele frequency- T:0.00NA 4546
    rs289442011,2,,4
    C,F,H--26147100(-) GAGGCC/TGCATG 2 R C mis1 ese39Minor allele frequency- T:0.00NS NA 4982
    rs2003778091,2
    C--26147132(+) CGGGCC/TGTTGT 1 -- int10--------
    rs1438846981,2
    C--26147135(+) GCTGTA/TGTTAC 1 -- int10--------
    rs2017043241,2
    C--26147143(+) TACCAC/TGTTGG 1 -- int10--------
    rs1925799531,2
    --26147146(+) CATGTG/TGGCCA 1 -- int10--------
    rs2011912011,2
    C--26147163(+) GGCGCA/C/TAGGGC 1 -- int10--------
    rs37297271,2
    C,F--26147226(-) AAGTGG/AGGATA 1 -- int117Minor allele frequency- A:0.19NS NA WA CSA EA 1122
    rs37296611,2
    C,F--26147355(-) AGTGGA/GGGTAA 1 -- int115Minor allele frequency- G:0.19NS NA WA CSA EA 1058
    rs118713151,2
    C,F,H--26147512(+) GCAGGC/TGCTGG 1 -- int11Minor allele frequency- T:0.50NA 4
    rs1405973911,2
    C--26147584(+) AGCCGT/CTGCTG 2 /Q syn11Minor allele frequency- C:0.00NA 2944
    rs1480881141,2
    C--26147673(+) GAAGCC/TGCTGG 2 S G mis10--------
    rs289442001,2
    C--26147677(-) AGTGCA/C/GAGCGG 3 A syn1 ese34NS 144
    rs1415799921,2
    --26147826(+) TCCTGC/TAGGCT 1 -- int10--------
    rs22559291,2
    C,F,H--26147877(+) TTCTGA/TAAAAG 1 -- int130Minor allele frequency- T:0.40EA NS NA MN WA CSA 3556
    rs19623801,2
    C,F,H--26147888(+) GGCCAA/TGCTCA 1 -- int114Minor allele frequency- T:0.22NA NS CSA WA EA 1020
    rs562043351,2
    --26147908(+) TGAGAA/CCCACC 1 -- int10--------
    rs37302341,2
    C,F--26147957(-) CACTGG/AGGTCA 1 -- int16Minor allele frequency- A:0.10NA WA EA 324
    rs2017886591,2
    C--26148031(+) GGAACA/C/TCCCAG 1 -- int10--------
    rs2003877561,2
    C--26148070(+) GGAAGC/GGGTGC 1 -- int10--------
    rs1995688431,2
    C--26148075(+) GGGTGC/TTTACT 1 -- int10--------
    rs1409148491,2
    C,F--26148113(+) GGGGCT/CTCAGG 2 /K /R mis11Minor allele frequency- C:0.00NA 4456
    rs1503871291,2
    C--26148147(+) GCCGTG/AGTGCA 2 /H /Y mis11Minor allele frequency- A:0.00NA 4426
    rs2015263251,2
    C--26148179(+) CGATAC/TCACAA 1 -- int10--------
    rs2007764481,2
    C--26148206(+) CCACTA/GCCCAT 1 -- int10--------
    rs37295071,2
    C,F--26148341(-) CCTCAG/CCTCCG 1 -- int17Minor allele frequency- C:0.04NS NA 728
    rs37947581,2
    C,F--26148426(+) AGTGAC/TTTGAA 1 -- int11Minor allele frequency- T:0.00NA 65
    rs1846516001,2
    --26148448(+) AGGTCA/GTGGTG 1 -- int10--------
    rs37947591,2
    C,F,A,H--26148644(+) ATGAGT/CTCCGC 1 -- int113Minor allele frequency- C:0.48NS NA WA CSA 680
    rs1477154191,2
    --26148672(+) GGCACC/TTTGCT 1 -- int10--------
    rs80681491,2
    C,F,A,H--26148764(+) CTTGAA/GGAAGG 1 -- int133Minor allele frequency- G:0.44NA NS EA WA CSA 3141
    rs1894375141,2
    --26148766(+) TGAGGA/GAGGCC 1 -- int10--------
    rs289441991,2
    C--26148795(-) AACCAG/CGGGGA 1 -- int14Minor allele frequency- C:0.00NS 132
    rs1408327521,2
    --26148808(+) CCTCGA/GGATTA 1 -- int10--------
    rs1446917891,2
    --26148834(+) GGACAC/TGTCTG 1 -- int10--------
    rs99061221,2
    C,F,A--26148970(+) TTGCCC/AACCCA 1 -- int112Minor allele frequency- A:0.46NS NA CSA EA 399
    rs289441981,2
    C,F--26149013(-) TGGCCG/TCTGCC 1 -- int17Minor allele frequency- T:0.04NS NA 162
    rs1489400141,2
    --26149043(+) CTGGAC/TGAGAT 1 -- int10--------
    rs1819525221,2
    --26149060(+) CTCCCA/GCTCTA 1 -- int10--------
    rs289441971,2
    C,F--26149073(-) GGTGAC/TGCTGC 1 -- int112Minor allele frequency- T:0.07NS NA CSA WA EA 1052
    rs289441961,2
    C,F--26149136(-) TGATTT/CTCATT 1 -- int19Minor allele frequency- C:0.38NS WA CSA NA 855
    rs289441951,2
    C,F--26149140(-) TAGATG/TATTTT 1 -- int18Minor allele frequency- T:0.21NS CSA NA 852
    rs289441941,2
    C,F--26149161(-) AGAGAT/GAGGCA 1 -- int18Minor allele frequency- G:0.03NS NA WA 194
    rs98958311,2
    C,F,A,H--26149174(+) ACTGCC/TGCCTC 1 -- int113Minor allele frequency- T:0.43NS NA CSA 750
    rs289441931,2
    C,F--26149223(-) TGGGTA/GTGGAA 1 -- int116Minor allele frequency- G:0.18NS NA WA CSA EA 1110
    rs1858294891,2
    --26149230(+) CCCACC/GCCAGT 1 -- int10--------
    rs1895035051,2
    --26149260(+) GCTGAA/GTGAGT 1 -- int10--------
    rs99068351,2
    C,F,A,H--26149283(+) AATGCA/GGAATC 1 -- int137Minor allele frequency- G:0.39NS EA NA WA CSA 3931
    rs767756571,2
    F--26149296(+) CCCTGC/TGTTCA 1 -- int11Minor allele frequency- T:0.01WA 118
    rs1811128131,2
    --26149312(+) CATGAG/TTGGGA 1 -- int10--------
    rs289441921,2
    C,F--26149319(-) TTAGTC/GTTCCC 1 -- int117Minor allele frequency- G:0.19NS NA WA CSA EA 1110
    rs1867585721,2
    --26149439(+) AGACTA/CTCCAG 1 -- int10--------
    rs289441901,2
    C,F--26149511(-) TAGGCA/CCTGTG 1 -- int111Minor allele frequency- C:0.08NS WA CSA NA EA 1098
    rs347490681,2
    F--26149575(+) TCCTAG/CAGATC 1 -- int11Minor allele frequency- C:0.50NA 4
    rs1168013781,2
    F--26149612(+) AACAAC/TTCTGG 1 -- int11Minor allele frequency- T:0.02WA 118
    rs289441891,2
    C,F--26149662(-) CTCAGA/GTTCAG 1 -- int15Minor allele frequency- G:0.00NS 186
    rs2021687501,2
    C--26149694(+) ATGGGC/TGGAGC 1 -- int10--------
    rs10608261,2
    C,F,H--26149776(-) CCCACG/AGAGAT 2 /T syn1 ese326Minor allele frequency- A:0.31NA NS WA CSA EA 4329
    rs2013815061,2
    C--26149865(+) CACCCA/GCAGGG 2 R W mis10--------
    rs1454722961,2
    C--26149872(+) AGGGAC/TGGGAA 2 P syn10--------
    rs1996039621,2
    C--26149975(+) AGGGCA/GCCTGT 1 -- int10--------
    rs289441881,2
    C,F--26150013(-) TGAATA/GCTGGG 1 -- int16Minor allele frequency- G:0.01NS CSA 164
    rs289441871,2
    C,F--26150076(-) GTCTCA/TGCACC 1 -- int15Minor allele frequency- T:0.01NS 160
    rs289441861,2
    C,F--26150135(-) ATAAAT/CGTACT 1 -- int17Minor allele frequency- C:0.15NS NA 832
    rs1831452321,2
    --26150206(+) TTTATG/TTGGGG 1 -- int10--------
    rs289441851,2
    C--26150271(-) TTCCAC/TAGCTA 1 -- int15Minor allele frequency- T:0.01NS 160
    rs289441841,2
    C--26150310(-) AATACT/CGTCCT 1 -- int15Minor allele frequency- C:0.01NS 160
    rs1443566881,2
    --26150395(+) ATGGC-/GG    
       GGGGG
    GGTGG
    1 -- int10--------
    rs289441831,2
    C--26150396(-) TCCACC/TGCCAT 1 -- int16Minor allele frequency- T:0.00NS 644
    rs1863591631,2
    --26150481(+) GAACCA/GGACAG 1 -- int10--------
    rs289441821,2
    C,F--26150533(-) ATGGAG/CAGCTG 1 -- int15Minor allele frequency- C:0.02NS 188
    rs289441811,2
    C,F--26150581(-) GGATGA/GTGTGG 1 -- int15Minor allele frequency- G:0.03NS 188
    rs22975101,2
    C,F,A--26150663(+) CCCTTC/TCTAGC 1 -- int1 trp310Minor allele frequency- T:0.31EA NA NS WA 1970
    rs1387935351,2
    C--26150960(+) TGTGGC/TCACCT 2 T A mis11Minor allele frequency- T:0.00NA 4326
    rs22975111,2
    C,F,A--26151136(+) CTCAGT/CAGGTA 1 -- int16Minor allele frequency- C:0.34NA NS 192
    rs289441801,2
    C,F--26151461(-) CAGGGG/AACTGC 1 -- int17Minor allele frequency- A:0.07NS WA 296
    rs1909083691,2
    --26151523(+) CAGGGA/GGCAGC 1 -- int10--------
    rs1841097291,2
    --26151568(+) GAAGAC/TACGGG 1 -- int10--------
    rs784048011,2
    F--26151570(+) AGACAC/TGGGTT 1 -- int11Minor allele frequency- T:0.02WA 118
    rs1141739081,2
    F--26151672(+) GCTGGG/TTTGGC 1 -- int11Minor allele frequency- T:0.01WA 118
    rs1874587221,2
    --26151756(+) TAAAAA/TTAAGA 1 -- int10--------
    rs92828001,2
    C,F--26151842(-) GGGGAC/TGACAA 1 -- int17Minor allele frequency- T:0.01MN NA 412
    rs80654481,2
    C,F--26151884(+) AGTGGG/AGGTCA 1 -- int17Minor allele frequency- A:0.04MN WA 528
    rs9447221,2
    C,F,A,H--26151946(+) GGAGTT/CTCTGT 1 -- int129Minor allele frequency- C:0.33NA EA MN NS WA CSA 1510
    rs1492567221,2
    --26151974(+) AGTGGC/TGAGAT 1 -- int10--------
    rs1444293791,2
    --26152112(+) GAGACA/GGGGTT 1 -- int10--------
    rs9447231,2
    C,F,A--26152170(+) CACCCG/ACCACG 1 -- int116Minor allele frequency- A:0.46NA MN NS WA CSA 755
    rs80658171,2
    C,F--26152238(+) TCTGAC/TTTTGG 1 -- int17Minor allele frequency- T:0.06NS WA 292
    rs37302321,2
    C,F,H--26152352(-) GAGGGC/TGGGCC 1 -- int19Minor allele frequency- T:0.00NS EA 606
    rs2015319411,2
    C--26152424(+) GGGGCA/GGCCAG 1 -- int10--------
    rs1829791191,2
    C--26152432(+) CAGAGA/GGTGCC 1 -- int10--------
    rs289441781,2
    C,F--26152461(-) AGTCCC/TGGGCT 1 -- int17Minor allele frequency- T:0.00NS EU 1687
    rs22975121,2
    C,F,A--26152464(+) CCGGGA/GCTCAC 1 -- int126Minor allele frequency- G:0.32EA NA NS WA CSA EU 3378
    rs1998924761,2
    C--26152484(+) CCAGGA/G/TCCTCC 3 D A V mis10--------
    rs2017953321,2
    --26152485(+) CAGGGA/CCTCCA 2 A S mis10--------
    rs10608221,2
    C,F--26152540(-) CAAGGC/TATCCT 2 G syn1 ese325Minor allele frequency- T:0.32EA MN NS NA WA CSA EU 7523
    rs1440578721,2
    C--26152553(+) CCAGGG/ACCGGC 2 /A /V mis11Minor allele frequency- A:0.00NA 3808
    rs2011052351,2
    C--26152571(+) CTGGGA/CAAACC 2 C F mis10--------
    rs1507042211,2
    C,F--26152649(+) TGGCAC/TGGCTG 2 H R mis12Minor allele frequency- T:0.00NA EU 5085
    rs289441771,2
    C,F--26152683(-) CTGCAA/GTGTCA 1 -- int16Minor allele frequency- G:0.01NS CSA 192
    rs289441761,2
    C--26152695(-) CTGGAG/AGAAAT 1 -- int15Minor allele frequency- A:0.01NS 190
    rs116551011,2
    H--26152875(+) CCCTTC/TCCAGC 1 -- int10--------
    rs22975131,2
    C,F--26152893(+) GGAGCC/TCCCCG 1 -- int117Minor allele frequency- T:0.12EA NS NA WA CSA 2584
    rs1842416461,2
    --26152914(+) TACAGA/CCTCTG 1 -- int10--------
    rs1138429591,2
    C--26153031(+) GCATGC/TGGGGA 1 -- int11Minor allele frequency- T:0.50NA 2
    rs1890004921,2
    --26153058(+) TGAAAC/TCTGCA 1 -- int10--------
    rs289441751,2
    C--26153195(-) TTCCTC/TATTGA 1 -- int1 trp35Minor allele frequency- T:0.00NS 188
    rs1408715041,2
    --26153201(+) AGGAAG/TGAGAG 1 -- int10--------
    rs22975141,2
    C,F,A,H--26153224(+) ATGCTT/CAGAGT 1 -- int129Minor allele frequency- C:0.49NS EA NA WA CSA 3098
    rs22975151,2
    C,F,H--26153242(+) GGAAAA/CGTGGC 1 -- int129Minor allele frequency- C:0.10NA NS EA 3424
    rs1930260891,2
    --26153270(+) TAGACA/GTAAAA 1 -- int10--------
    rs1857081321,2
    --26153333(+) GCTAAC/TGCCAG 1 -- int10--------
    rs1508639911,2
    C--26153401(+) AAATAA/GAATCG 1 -- int10--------
    rs2003826281,2
    C--26153406(+) AAATCA/GACAGA 1 -- int10--------
    rs2003911261,2
    --26153415(+) GAGTAC/TCACCC 1 -- int10--------
    rs289441741,2
    C--26153422(-) GCTTCG/ATGGGT 1 -- int15Minor allele frequency- A:0.00NS 188
    rs289441731,2,,4
    C,F,H--26153452(-) GTCCGA/GCATCC 2 T A mis1 ese313Minor allele frequency- G:0.02NS EA NA 870
    rs2007795741,2
    C--26153532(+) TGGAGG/AACACA 1 -- int11Minor allele frequency- A:0.00EU 1323
    rs2012822911,2
    --26153567(+) TCTTGA/GGCGTA 1 -- int10--------
    rs1891668501,2
    --26153647(+) TGGCCA/GTTGGG 1 -- int10--------
    rs289441721,2
    C--26153653(-) GAAAAA/GCCCAA 1 -- int1 trp35Minor allele frequency- G:0.01NS 160
    rs1392955581,2
    --26153685(+) AAATAA/GAGATC 1 -- int10--------
    rs47960341,2
    C,F,A,H--26153701(+) TCCAGA/GCCGGG 1 -- int119Minor allele frequency- G:0.35NA NS WA CSA EA 1027
    rs289441711,2
    C--26153736(-) CAAAAG/ATGCTG 1 -- int16Minor allele frequency- A:0.00NS 648
    rs286622101,2
    C,F--26153773(+) GGTCAG/AGAGTT 1 -- int120Minor allele frequency- A:0.35NA NS WA CSA EA 1027
    rs114284601,2
    C--26153935(+) GTCTC-/AAAAAA 1 -- int1 trp31Minor allele frequency- A:0.50NA 2
    rs1465380081,2
    --26154002(+) TTCTAC/GGTGTT 1 -- int10--------
    rs47960381,2
    C,F--26154187(+) CACTAC/AAGCCT 1 -- int110Minor allele frequency- A:0.20NS NA WA 170
    rs1810438701,2
    --26154198(+) GGAATC/TGCCTC 1 -- int10--------
    rs289441701,2
    C,F--26154318(-) CAGATG/AGAGAA 1 -- int15Minor allele frequency- A:0.01NS 170
    rs1464893001,2
    --26154495(+) TATGCC/TCTAAC 1 -- int10--------
    rs289441691,2
    C,F--26154504(-) CAAGAG/ACCTGT 1 -- int16Minor allele frequency- A:0.03NS 654
    rs2016449531,2
    C--26154635(+) AGCAGC/GTACCT 1 -- int10--------
    rs2007892181,2
    --26154668(+) AGTCCG/TGCACG 2 Q P mis10--------
    rs3720870261,2
    C--26154673(+) TGCACA/GAGCCT 2 L syn10--------
    rs289441681,2
    C--26154688(-) GACCCG/ACACCA 2 /P syn1 ese35Minor allele frequency- A:0.00NS 186
    rs1468521001,2
    C,F--26154704(+) TCACAT/CTGGAG 2 /N /S mis11Minor allele frequency- C:0.00NA 4552
    rs1856343081,2
    --26154716(+) TGTAGA/GGCTTG 2 P L mis10--------
    rs37302301,2
    H--26154753(-) CGTTTG/CATGTC 2 /H /D mis1 ese34Minor allele frequency- C:0.00NS EA 418
    rs2015635431,2
    C--26154757(+) TCAAAC/TGTCTC 2 T syn11Minor allele frequency- T:0.00EU 1323
    rs3751186221,2
    C--26154795(+) GACTGC/TGGTTA 1 -- int10--------
    rs1996253221,2
    C--26154807(+) TGGTCA/GGCAGC 1 -- int10--------
    rs1482269641,2
    --26154927(+) AGCAAA/GGACTC 1 -- int10--------
    rs118711501,2
    C,F,H--26155023(+) cagtcG/Acccag 1 -- int11Minor allele frequency- A:0.50NA 4
    rs113637391,2
    C--26155103(+) CTCAGT/-CCCGT 1 -- int11Minor allele frequency- -:0.00NA 2
    rs3689421241,2
    C--26155103(+) CTCAG-/TC    
       CCGTA
    CCTGT
    1 -- int10--------
    rs105695341,2
    C--26155106(+) agtcc-/CGtacct 1 -- int10--------
    rs113637401,2
    C--26155109(+) CCCGT-/ACCTGT 1 -- int10--------
    rs2011368721,2
    C--26155112(+) TACCT-/GAGTAGC 1 -- int10--------
    rs1145313171,2
    F--26155145(+) CAAGCC/TCAGCC 1 -- int11Minor allele frequency- T:0.02WA 118
    rs1401767441,2
    --26155278(+) CTGTGC/TCCGAC 1 -- int10--------
    rs1887771171,2
    --26155283(+) CCCGAA/CCACTA 1 -- int10--------
    rs23148091,2
    C,F,O,A,H--26155286(+) GACCAC/TTATGA 1 -- int141Minor allele frequency- N:0.00NS EA NA WA CSA 3569
    rs1813381721,2
    C--26155352(+) GATTGA/C/TTCTTA 1 -- int10--------
    rs1503011721,2
    --26155415(+) GCCCCA/GATGCA 1 -- int10--------
    rs1859578301,2
    --26155417(+) CCCGAC/TGCAAG 1 -- int10--------
    rs28727531,2
    C,F,O,A,H--26155470(+) GTGACA/GAGTTC 1 -- int143Minor allele frequency- G:0.37NS EA NA WA CSA 4067
    rs22975161,2
    C,F,A,H--26155638(+) AGGAAA/CGACCT 1 -- int1 trp338Minor allele frequency- C:0.48EA NS NA MN CSA WA 5195
    rs1906708471,2
    --26155849(+) AGATGC/TCCTAG 1 -- int10--------
    rs1995239991,2
    C--26155874(+) TGTCAC/TGTCCC 1 -- int10--------
    rs1406488181,2
    C,F--26155926(+) TGCACG/AGCCCA 2 /A syn11Minor allele frequency- A:0.00NA 4474
    rs1440983301,2
    C--26155944(+) AAGGCA/GTCCTC 2 D syn10--------
    rs1836455301,2
    C--26155981(+) CCATCA/GGGGTG 2 P L mis10--------
    rs2015314641,2
    C--26156039(+) AAAGGC/TGCAGA 2 T A mis10--------
    rs2020981321,2
    C--26156040(+) AAGGCA/GCAGAA 2 C syn10--------
    rs2015009561,2
    C--26156092(+) TGCCCA/GAGGAC 1 -- int10--------
    rs1876613061,2
    --26156176(+) GCCCCA/GGACTC 1 -- int10--------
    rs22975171,2
    C,F,H--26156228(+) CCTCTC/TCATCC 1 -- int111Minor allele frequency- T:0.03EA NS NA 2150
    rs289994161,2
    C,F--26156264(-) CATGGG/AGTGGT 1 -- int16Minor allele frequency- A:0.00NS 728
    rs1494118881,2
    C--26156310(+) TGTGGC/TTTGAC 1 -- int10--------
    rs92828011,2
    C,F--26156381(-) CAGGGG/TGTCTT 1 -- int122Minor allele frequency- T:0.32MN NS NA WA CSA EA 1586
    rs2007148031,2
    C--26156454(+) GACAAC/TGGAAA 1 -- int10--------
    rs2000182541,2
    --26156487(+) TGAGCC/TCTTTC 2 E G mis10--------
    rs22975181,2,,4
    C,F,H--26156505(+) AGAGCG/AATTTC 2 /S /L mis148Minor allele frequency- A:0.15EA MN NA NS WA EU 11447
    rs2020448891,2
    --26156535(+) GGGAAC/TGACAG 1 -- int10--------
    rs1907252631,2
    --26156611(+) TGCAGA/GAGAAG 1 -- int10--------
    rs37302281,2
    C,F,H--26156632(-) CTTCCA/G/TTGGTG 1 -- int127MN NS EA NA WA 2278
    rs289994151,2
    C,F--26156711(-) TTTAGT/CTGGAG 1 -- int15Minor allele frequency- C:0.00NS 188
    rs1867322381,2
    --26156786(+) TCCAGA/CCCTGG 1 -- int10--------
    rs1915406401,2
    --26156801(+) GGGGCC/GTGCCT 1 -- int10--------
    rs1447782381,2
    --26156815(+) AGCATA/GCCTTC 1 -- int10--------
    rs1485382561,2
    --26156830(+) GAGGGA/GCTCCG 1 -- int10--------
    rs1428579551,2
    --26156840(+) GAATGA/CGCCGT 1 -- int10--------
    rs97972441,2
    C,F,A,H--26157039(+) TTCATT/CCTTCG 1 -- int138Minor allele frequency- C:0.20NS EA NA MN WA CSA 3364
    rs289994141,2
    C,F--26157289(-) GCACGC/TGCCAC 1 -- int16Minor allele frequency- T:0.01NS 746
    rs1825703651,2
    --26157369(+) GAGCTA/GAGATC 1 -- int10--------
    rs47960401,2
    C,F,A,H--26157388(+) gcactG/Ctaccc 1 -- int118Minor allele frequency- C:0.41NA NS WA CSA EA 1003
    rs1400047671,2
    --26157412(+) CAAAAC/GTCCAC 1 -- int10--------
    rs1386570941,2
    C--26157423(+) CTAAG-/TAAA  
            
    TAAAT
    1 -- int10--------
    rs1872212391,2
    --26157438(+) AATAAA/GTAAAC 1 -- int1