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Aliases for NOS1 Gene

Aliases for NOS1 Gene

  • Nitric Oxide Synthase 1 2 3 5
  • Peptidyl-Cysteine S-Nitrosylase NOS1 3 4
  • Nitric Oxide Synthase 1 (Neuronal) 2 3
  • Constitutive NOS 3 4
  • EC 1.14.13.39 4 63
  • Neuronal NOS 3 4
  • NOS Type I 3 4
  • NC-NOS 3 4
  • N-NOS 3 4
  • BNOS 3 4
  • NNOS 3 4
  • IHPS1 3
  • NOS 3

External Ids for NOS1 Gene

Previous HGNC Symbols for NOS1 Gene

  • NOS

Previous GeneCards Identifiers for NOS1 Gene

  • GC12M116666
  • GC12M117431
  • GC12M116060
  • GC12M116111
  • GC12M117636
  • GC12M114660

Summaries for NOS1 Gene

Entrez Gene Summary for NOS1 Gene

  • The protein encoded by this gene belongs to the family of nitric oxide synthases, which synthesize nitric oxide from L-arginine. Nitric oxide is a reactive free radical, which acts as a biologic mediator in several processes, including neurotransmission, and antimicrobial and antitumoral activities. In the brain and peripheral nervous system, nitric oxide displays many properties of a neurotransmitter, and has been implicated in neurotoxicity associated with stroke and neurodegenerative diseases, neural regulation of smooth muscle, including peristalsis, and penile erection. This protein is ubiquitously expressed, with high level of expression in skeletal muscle. Multiple transcript variants that differ in the 5' UTR have been described for this gene but the full-length nature of these transcripts is not known. Additionally, alternatively spliced transcript variants encoding different isoforms (some testis-specific) have been found for this gene.[provided by RefSeq, Feb 2011]

GeneCards Summary for NOS1 Gene

NOS1 (Nitric Oxide Synthase 1) is a Protein Coding gene. Diseases associated with NOS1 include Achalasia and Spermatogenic Failure 12. Among its related pathways are Neuropathic Pain-Signaling in Dorsal Horn Neurons and Arginine biosynthesis. GO annotations related to this gene include oxidoreductase activity and iron ion binding. An important paralog of this gene is MTRR.

UniProtKB/Swiss-Prot for NOS1 Gene

  • Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR.

Tocris Summary for NOS1 Gene

  • Neuronal nitric oxide synthase (nNOS), with inducible nitric oxide synthase (iNOS) and endothelial nitric oxide synthase (eNOS), catalyzes nitric oxide and L-citrulline generation from L-arginine and molecular oxygen. nNOS is activated at calcium concentrations over 100 nM.

Gene Wiki entry for NOS1 Gene

No data available for PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for NOS1 Gene

Genomics for NOS1 Gene

Regulatory Elements for NOS1 Gene

Enhancers for NOS1 Gene
GeneHancer Identifier Score Enhancer Sources TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor Binding Sites within enhancer Other Gene Targets for Enhancer

Enhancers around NOS1 on UCSC Golden Path with GeneCards custom track

Genomic Location for NOS1 Gene

Chromosome:
12
Start:
117,208,142 bp from pter
End:
117,452,170 bp from pter
Size:
244,029 bases
Orientation:
Minus strand

Genomic View for NOS1 Gene

Genes around NOS1 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
NOS1 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for NOS1 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for NOS1 Gene

Proteins for NOS1 Gene

  • Protein details for NOS1 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P29475-NOS1_HUMAN
    Recommended name:
    Nitric oxide synthase, brain
    Protein Accession:
    P29475
    Secondary Accessions:
    • E9PH30
    • O75713

    Protein attributes for NOS1 Gene

    Size:
    1434 amino acids
    Molecular mass:
    160970 Da
    Cofactor:
    Name=5,6,7,8-tetrahydrobiopterin; Xref=ChEBI:CHEBI:15372;
    Cofactor:
    Name=FAD; Xref=ChEBI:CHEBI:57692;
    Cofactor:
    Name=FMN; Xref=ChEBI:CHEBI:58210;
    Cofactor:
    Name=heme; Xref=ChEBI:CHEBI:30413;
    Quaternary structure:
    • Homodimer. Interacts with DLG4; the interaction possibly being prevented by the association between NOS1 and CAPON. Forms a ternary complex with CAPON and RASD1. Forms a ternary complex with CAPON and SYN1. Interacts with ZDHHC23. Interacts with NOSIP; which may impair its synaptic location (By similarity). Interacts with HTR4. Interacts with VAC14 (By similarity). Interacts with SLC6A4 (By similarity). Interacts (via N-terminal domain) with DLG4 (via N-terminal tandem pair of PDZ domains) (By similarity).

    Three dimensional structures from OCA and Proteopedia for NOS1 Gene

    Alternative splice isoforms for NOS1 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for NOS1 Gene

Proteomics data for NOS1 Gene at MOPED

Post-translational modifications for NOS1 Gene

  • Ubiquitinated; mediated by STUB1/CHIP in the presence of Hsp70 and Hsp40 (in vitro).
  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

Other Protein References for NOS1 Gene

Antibody Products

  • Cell Signaling Technology (CST) Antibodies for NOS1 (nNOS)

Domains & Families for NOS1 Gene

Gene Families for NOS1 Gene

Graphical View of Domain Structure for InterPro Entry

P29475

UniProtKB/Swiss-Prot:

NOS1_HUMAN :
  • The PDZ domain in the N-terminal part of the neuronal isoform participates in protein-protein interaction, and is responsible for targeting nNos to synaptic membranes in muscles. Mediates interaction with VAC14 (By similarity).
  • Belongs to the NOS family.
Domain:
  • The PDZ domain in the N-terminal part of the neuronal isoform participates in protein-protein interaction, and is responsible for targeting nNos to synaptic membranes in muscles. Mediates interaction with VAC14 (By similarity).
  • Contains 1 FAD-binding FR-type domain.
  • Contains 1 flavodoxin-like domain.
  • Contains 1 PDZ (DHR) domain.
Family:
  • Belongs to the NOS family.
genes like me logo Genes that share domains with NOS1: view

Function for NOS1 Gene

Molecular function for NOS1 Gene

GENATLAS Biochemistry:
nitric oxide synthase 1,constitutive by expressed,neuronal,putative susceptibility gene for infantile pyloric stenosis,widely expressed with a high expression in skeletal muscle
UniProtKB/Swiss-Prot CatalyticActivity:
2 L-arginine + 3 NADPH + 4 O(2) = 2 L-citrulline + 2 nitric oxide + 3 NADP(+) + 4 H(2)O.
UniProtKB/Swiss-Prot EnzymeRegulation:
Stimulated by calcium/calmodulin. Inhibited by n-Nos-inhibiting protein (PIN) which may prevent the dimerization of the protein. Inhibited by NOSIP.
UniProtKB/Swiss-Prot Function:
Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR.

Enzyme Numbers (IUBMB) for NOS1 Gene

Gene Ontology (GO) - Molecular Function for NOS1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005506 iron ion binding IEA --
GO:0005515 protein binding IEA,IPI 11149895
GO:0020037 heme binding IEA,ISS --
GO:0050661 NADP binding IEA,ISS --
genes like me logo Genes that share ontologies with NOS1: view
genes like me logo Genes that share phenotypes with NOS1: view

Human Phenotype Ontology for NOS1 Gene

HPO Id HPO Name Alternative Ids Definition Synonyms

Animal Models for NOS1 Gene

MGI Knock Outs for NOS1:

Animal Model Products

miRNA for NOS1 Gene

miRTarBase miRNAs that target NOS1

No data available for Transcription Factor Targets and HOMER Transcription for NOS1 Gene

Localization for NOS1 Gene

Subcellular locations from UniProtKB/Swiss-Prot for NOS1 Gene

Cell membrane, sarcolemma; Peripheral membrane protein. Cell projection, dendritic spine. Note=In skeletal muscle, it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex. In neurons, enriched in dendritic spines (By similarity). {ECO:0000250}.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for NOS1 Gene COMPARTMENTS Subcellular localization image for NOS1 gene
Compartment Confidence
cytosol 5
endoplasmic reticulum 5
plasma membrane 5
cytoskeleton 4
mitochondrion 4
nucleus 4
extracellular 2
lysosome 2
vacuole 2

Gene Ontology (GO) - Cellular Components for NOS1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0001917 photoreceptor inner segment ISS 17027776
GO:0005829 cytosol IEA,TAS --
GO:0005886 plasma membrane IEA --
GO:0012506 vesicle membrane IEA --
GO:0030315 T-tubule IEA --
genes like me logo Genes that share ontologies with NOS1: view

Pathways & Interactions for NOS1 Gene

genes like me logo Genes that share pathways with NOS1: view

Gene Ontology (GO) - Biological Process for NOS1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0001666 response to hypoxia IEP 16276418
GO:0006809 nitric oxide biosynthetic process IEA,ISS 7545544
GO:0007263 nitric oxide mediated signal transduction IBA --
GO:0007565 female pregnancy IEA --
GO:0007568 aging IEA --
genes like me logo Genes that share ontologies with NOS1: view

No data available for SIGNOR curated interactions for NOS1 Gene

Drugs & Compounds for NOS1 Gene

(102) Drugs for NOS1 Gene - From: DrugBank, ApexBio, DGIdb, FDA Approved Drugs, IUPHAR, HMDB, Tocris, and Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Citrulline Approved Nutra Full agonist, Agonist, Target 0
FAD Approved Pharma Target 0
Nitric Oxide Approved Pharma 576
Tetrahydrobiopterin Approved, Investigational Pharma 0
Doxorubicin Approved, Investigational Pharma Enzyme, substrate Topo II inhibitor,immunosuppresive antineoplastic antibiotic 1649

(65) Additional Compounds for NOS1 Gene - From: Novoseek, HMDB, and Tocris

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
nadph
  • 2'-(Dihydrogen phosphate) 5'-(trihydrogen pyrophosphate) Adenosine 5'-ester with 1,4-dihydro-1-b-D-ribofuranosylnicotinamide
  • 2'-(Dihydrogen phosphate) 5'-(trihydrogen pyrophosphate) Adenosine 5'-ester with 1,4-dihydro-1-beta-delta-ribofuranosylnicotinamide
  • Adenosine 5'-(trihydrogen diphosphate) 2'-(dihydrogen phosphate) P'-5'-ester with 1,4-dihydro-1-beta-D-ribofuranosyl-3-pyridinecarboxamide
  • Adenosine 5'-(trihydrogen diphosphate) 2'-(dihydrogen phosphate) P'-5'-ester with 1,4-dihydro-1-beta-delta-ribofuranosyl-3-pyridinecarboxamide
  • b-NADPH
53-57-6
oxygen
  • Dioxygen
  • Molecular oxygen
  • O2
  • Oxygen
  • Oxygen molecule
7782-44-7
D-Mannose
  • (+)-mannose
  • (+-)-mannose
  • Carubinose
  • D(+)-Mannose
  • D-Mannopyranose
3458-28-4
Hydrogen Ion
  • Hydrogen cation
  • Hydron
  • Proton
N-(o)-Hydroxyarginine
  • N-(G)-Hydroxyarginine
  • N-(Omega)-Hydroxyarginine

(5) Tocris Compounds for NOS1 Gene

Compound Action Cas Number
3-Bromo-7-nitroindazole Selective nNOS inhibitor 74209-34-0
ARL 17477 dihydrochloride Selective nNOS inhibitor 866914-87-6
L-NIO dihydrochloride NOS inhibitor 159190-44-0
Nomega-Propyl-L-arginine hydrochloride Highly selective inhibitor of nNOS 137361-05-8
Pentamidine isethionate Antimicrobial that antagonizes NMDA receptors 140-64-7

(1) ApexBio Compounds for NOS1 Gene

Compound Action Cas Number
1400W dihydrochloride INOS inhibitor,potent and highly selective 214358-33-5
genes like me logo Genes that share compounds with NOS1: view

Drug Products

Transcripts for NOS1 Gene

Alternative Splicing Database (ASD) splice patterns (SP) for NOS1 Gene

No ASD Table

Relevant External Links for NOS1 Gene

GeneLoc Exon Structure for
NOS1
ECgene alternative splicing isoforms for
NOS1

Expression for NOS1 Gene

mRNA expression in normal human tissues for NOS1 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for NOS1 Gene

This gene is overexpressed in Muscle - Skeletal (x8.8), Brain - Putamen (basal ganglia) (x5.7), and Brain - Caudate (basal ganglia) (x4.6).

Protein differential expression in normal tissues from HIPED for NOS1 Gene

This gene is overexpressed in Fetal gut (18.3), Pancreas (17.1), Lung (12.1), and Colon (6.4).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, and MOPED for NOS1 Gene



SOURCE GeneReport for Unigene cluster for NOS1 Gene Hs.654410

mRNA Expression by UniProt/SwissProt for NOS1 Gene

P29475-NOS1_HUMAN
Tissue specificity: Isoform 1 is ubiquitously expressed: detected in skeletal muscle and brain, also in testis, lung and kidney, and at low levels in heart, adrenal gland and retina. Not detected in the platelets. Isoform 3 is expressed only in testis. Isoform 4 is detected in testis, skeletal muscle, lung, and kidney, at low levels in the brain, but not in the heart and adrenal gland.
genes like me logo Genes that share expression patterns with NOS1: view

Primer Products

No data available for Protein tissue co-expression partners for NOS1 Gene

Orthologs for NOS1 Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for NOS1 Gene

Organism Taxonomy Gene Similarity Type Details
cow
(Bos Taurus)
Mammalia NOS1 35
  • 90.34 (n)
  • 95.02 (a)
NOS1 36
  • 95 (a)
OneToOne
dog
(Canis familiaris)
Mammalia NOS1 35
  • 90.4 (n)
  • 94.41 (a)
NOS1 36
  • 94 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Nos1 35
  • 87.05 (n)
  • 94.05 (a)
Nos1 16
Nos1 36
  • 94 (a)
OneToOne
chimpanzee
(Pan troglodytes)
Mammalia NOS1 35
  • 98.48 (n)
  • 98.45 (a)
NOS1 36
  • 99 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Nos1 35
  • 87.45 (n)
  • 94.05 (a)
oppossum
(Monodelphis domestica)
Mammalia NOS1 36
  • 91 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia -- 36
  • 93 (a)
OneToMany
-- 36
  • 74 (a)
OneToMany
-- 36
  • 84 (a)
OneToMany
chicken
(Gallus gallus)
Aves NOS1 35
  • 80.04 (n)
  • 84.36 (a)
NOS1 36
  • 84 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia NOS1 36
  • 81 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia nos1 35
  • 71.82 (n)
  • 79.24 (a)
African clawed frog
(Xenopus laevis)
Amphibia nos1-A 35
zebrafish
(Danio rerio)
Actinopterygii nos1 35
  • 70.83 (n)
  • 74.82 (a)
nos1 36
  • 74 (a)
OneToOne
fruit fly
(Drosophila melanogaster)
Insecta Nos 37
  • 49 (a)
Nos 35
  • 56.44 (n)
  • 51.37 (a)
Nos 36
  • 45 (a)
OneToMany
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP008255 35
  • 57.58 (n)
  • 52.9 (a)
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes NCP1 36
  • 25 (a)
OneToMany
sea squirt
(Ciona intestinalis)
Ascidiacea Cin.12824 35
sea squirt
(Ciona savignyi)
Ascidiacea CSA.5751 36
  • 54 (a)
OneToMany
Species with no ortholog for NOS1:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for NOS1 Gene

ENSEMBL:
Gene Tree for NOS1 (if available)
TreeFam:
Gene Tree for NOS1 (if available)

Paralogs for NOS1 Gene

Paralogs for NOS1 Gene

(2) SIMAP similar genes for NOS1 Gene using alignment to 5 proteins:

genes like me logo Genes that share paralogs with NOS1: view

Variants for NOS1 Gene

Sequence variations from dbSNP and Humsavar for NOS1 Gene

SNP ID Clin Chr 12 pos Sequence Context AA Info Type
rs9658279 - 117,330,388(-) GGGCA(C/T)CTGCC reference, missense
rs9658356 - 117,286,213(-) GATCG(A/C)CACCA reference, missense
rs9658403 - 117,263,938(-) GCACC(A/G)ACGGG reference, missense
rs9658445 - 117,253,695(-) ATCAG(A/G)CGATG reference, missense
rs9658482 - 117,234,609(-) CAACC(A/G)GATGG reference, missense

Structural Variations from Database of Genomic Variants (DGV) for NOS1 Gene

Variant ID Type Subtype PubMed ID
nsv832526 CNV Loss 17160897
nsv510592 CNV Loss 20534489
nsv53465 CNV Loss 16902084
esv2746435 CNV Deletion 23290073
esv2746436 CNV Deletion 23290073
nsv509478 CNV Insertion 20534489
nsv901 CNV Insertion 18451855

Variation tolerance for NOS1 Gene

Residual Variation Intolerance Score: 5.31% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 11.81; 93.40% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for NOS1 Gene

Human Gene Mutation Database (HGMD)
NOS1

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for NOS1 Gene

Disorders for NOS1 Gene

MalaCards: The human disease database

(30) MalaCards diseases for NOS1 Gene - From: OMIM, Orphanet, DISEASES, Novoseek, and GeneCards

Disorder Aliases PubMed IDs
achalasia
  • achalasia of cardia
spermatogenic failure 12
  • spgf12
pyloric stenosis
impotence
  • erectile dysfunction
hypertrophic pyloric stenosis
  • congenital hypertrophic pyloric stenosis
- elite association - COSMIC cancer census association via MalaCards
Search NOS1 in MalaCards View complete list of genes associated with diseases

Relevant External Links for NOS1

Genetic Association Database (GAD)
NOS1
Human Genome Epidemiology (HuGE) Navigator
NOS1
Atlas of Genetics and Cytogenetics in Oncology and Haematology:
NOS1
genes like me logo Genes that share disorders with NOS1: view

No data available for UniProtKB/Swiss-Prot and Genatlas for NOS1 Gene

Publications for NOS1 Gene

  1. Genetic association analysis of functional polymorphisms in neuronal nitric oxide synthase 1 gene (NOS1) and mood disorders and fluvoxamine response in major depressive disorder in the Japanese population. (PMID: 20016223) Okumura T. … Iwata N. (Neuropsychobiology 2010) 3 23 48 67
  2. Cu2+ and Zn2+ inhibit nitric-oxide synthase through an interaction with the reductase domain. (PMID: 10799481) Perry J.M. … Marletta M.A. (J. Biol. Chem. 2000) 23 25 26
  3. Arginine conversion to nitroxide by tetrahydrobiopterin-free neuronal nitric-oxide synthase. Implications for mechanism. (PMID: 10945985) Adak S. … Stuehr D.J. (J. Biol. Chem. 2000) 23 25 26
  4. Localization of brain nitric oxide synthase (NOS) to human chromosome 12. (PMID: 1385308) Kishimoto J. … Xu W. (Genomics 1992) 2 3 23
  5. Lys842 in neuronal nitric-oxide synthase enables the autoinhibitory insert to antagonize calmodulin binding, increase FMN shielding, and suppress interflavin electron transfer. (PMID: 19948738) Guan Z.W. … Stuehr D.J. (J. Biol. Chem. 2010) 3 23

Products for NOS1 Gene

Sources for NOS1 Gene

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