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NONO Gene

protein-coding   GIFtS: 63
GCID: GC0XP070503

Non-POU Domain Containing, Octamer-Binding

(Previous names: non-POU-domain-containing, octamer-binding)
  See NONO-related diseases
at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
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Aliases
Non-POU Domain Containing, Octamer-Binding1 2     54-kD1
NRB542 3 5     Non-POU-Domain-Containing, Octamer-Binding1
Non-Pou Domain-Containing Octamer (ATGCAAAT) Binding Protein1 2     Nuclear RNA-Binding Protein1
54 KDa Nuclear RNA- And DNA-Binding Protein2 3     P542
55 KDa Nuclear Protein2 3     P54NRB2
NMT552 3     Non-POU Domain-Containing Octamer-Binding Protein2
DNA-Binding P52/P100 Complex, 52 KDa Subunit2 3     NonO Protein3
p54(nrb)2 3     p54nrb3

External Ids:    HGNC: 78711   Entrez Gene: 48412   Ensembl: ENSG000001471407   OMIM: 3000845   UniProtKB: Q152333   

Export aliases for NONO gene to outside databases

Previous GC identifers: GC0XP066677 GC16M070209 GC16M069524 GC16M069565 GC0XP069370 GC0XP070286 GC0XP070420 GC0XP064319


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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Entrez Gene summary for NONO Gene:
This gene encodes an RNA-binding protein which plays various roles in the nucleus, including transcriptional
regulation and RNA splicing. A rearrangement between this gene and the transcription factor E3 gene has been
observed in papillary renal cell carcinoma. Alternatively spliced transcript variants have been described.
Pseudogenes exist on Chromosomes 2 and 16. (provided by RefSeq, Feb 2009)

GeneCards Summary for NONO Gene:
NONO (non-POU domain containing, octamer-binding) is a protein-coding gene. Diseases associated with NONO include renal cell carcinoma, papillary, 1, and papillary renal cell carcinoma. GO annotations related to this gene include RNA binding and nucleotide binding. An important paralog of this gene is PSPC1.

UniProtKB/Swiss-Prot: NONO_HUMAN, Q15233
Function: DNA- and RNA binding protein, involved in several nuclear processes. Binds the conventional octamer
sequence in double-stranded DNA. Also binds single-stranded DNA and RNA at a site independent of the duplex site
(By similarity). Involved in pre-mRNA splicing, probably as a heterodimer with SFPQ. Interacts with U5 snRNA,
probably by binding to a purine-rich sequence located on the 3' side of U5 snRNA stem 1b. The SFPQ-NONO heteromer
associated with MATR3 may play a role in nuclear retention of defective RNAs. The SFPQ-NONO heteromer may be
involved in DNA unwinding by modulating the function of topoisomerase I/TOP1. The SFPQ-NONO heteromer may be
involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination
and may stabilize paired DNA ends. In vitro, the complex strongly stimulates DNA end joining, binds directly to
the DNA substrates and cooperates with the Ku70/G22P1-Ku80/XRCC5 (Ku) dimer to establish a functional preligation
complex. NONO is involved in transcriptional regulation. The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter
and regulates basal and cAMP-dependent transcriptional avtivity. NONO binds to an enhancer element in long
terminal repeats of endogenous intracisternal A particles (IAPs) and activates transcription (By similarity).
Together with PSPC1, required for the formation of nuclear paraspeckles

Gene Wiki entry for NONO Gene


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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RefSeq DNA sequence at NCBI GenBank:
NC_000023.10  NC_018934.2  NT_011651.18  
Regulatory elements:
   Regulatory transcription factor binding sites in the NONO gene promoter:
         CREB   POU2F1   POU2F1a   deltaCREB   ARP-1   YY1   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmids (see all 7): NONO promoter sequence
   Search Chromatin IP Primers for NONO

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat NONO


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: Xq13.1   Ensembl cytogenetic band:  Xq13.1   HGNC cytogenetic band: Xq13.1

NONO Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
NONO gene location

GeneLoc information about chromosome X         GeneLoc Exon Structure

GeneLoc location for GC0XP070503:  view genomic region     (about GC identifiers)

Start:
70,503,042 bp from pter      End:
70,521,018 bp from pter
Size:
17,977 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, Cloud-Clone Corp., eBioscience, and/or antibodies-online,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, Cloud-Clone Corp., eBioscience, and/or antibodies-online, Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, Cloud-Clone Corp, antibodies-online, and/or others.)
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UniProtKB/Swiss-Prot: NONO_HUMAN, Q15233 (See protein sequence)
Recommended Name: Non-POU domain-containing octamer-binding protein  
Size: 471 amino acids; 54232 Da
Subunit: Monomer and component of the SFPQ-NONO complex, which is probably a heterotetramer of two 52 kDa (NONO)
and two 100 kDa (SFPQ) subunits. NONO is a component of spliceosome and U5.4/6 snRNP complexes. Interacts with
PSPC1 and SNRPA/U1A. Part of complex consisting of SFPQ, NONO and MATR3. Part of a complex consisting of SFPQ,
NONO and NR5A1. Part of a complex consisting of SFPQ, NONO and TOP1. Interacts with SPI1 (By similarity).
Interacts with RNF43. Forms heterodimers with PSPC1; this involves formation of a coiled coil domain by helices
from both proteins
1 PDB 3D structure from and Proteopedia for NONO:
3SDE (3D)    
Secondary accessions: B7Z4C2 D3DVV4 F5GYZ3 O00201 P30807 Q12786 Q9BQC5
Alternative splicing: 2 isoforms:  Q15233-1   Q15233-2   (No experimental confirmation available)

Explore the universe of human proteins at neXtProt for NONO: NX_Q15233

Explore proteomics data for NONO at MOPED

Post-translational modifications: 

  • The N-terminus is blocked1
  • Ubiquitination2 at Lys11, Lys68, Lys99, Lys107, Lys109, Lys126, Lys198, Lys239, Lys243, Lys272,
                                 Lys279, Lys295, Lys336, Lys338, Lys371
  • Modification sites at PhosphoSitePlus

  • See NONO Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins (4 alternative transcripts): 
    NP_001138880.1  NP_001138881.1  NP_001138882.1  NP_031389.3  

    ENSEMBL proteins: 
     ENSP00000276079   ENSP00000362963   ENSP00000362947   ENSP00000410299   ENSP00000413350  
     ENSP00000415777   ENSP00000406673   ENSP00000409773   ENSP00000441364  

    NONO Human Recombinant Protein Products:

    Browse Purified and Recombinant Proteins at EMD Millipore
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    OriGene Purified Proteins for NONO
    OriGene Protein Over-expression Lysate for NONO
    OriGene MassSpec for NONO
    OriGene Custom Protein Services for NONO
    GenScript Custom Purified and Recombinant Proteins Services for NONO
    Novus Biologicals NONO Protein
    Novus Biologicals NONO Lysates
    Browse Sino Biological Recombinant Proteins
    Browse Sino Biological Cell Lysates
    Browse ProSpec Recombinant Proteins
    Cloud-Clone Corp. Proteins for NONO

     
    Search eBioscience for Proteins for NONO 

     
    antibodies-online proteins for NONO (5 products) 

     
    antibodies-online peptides for NONO

    NONO Antibody Products:

    EMD Millipore Mono- and Polyclonal Antibodies for the study of NONO
    Browse R&D Systems for Antibodies
    OriGene Antibodies for NONO
    OriGene Custom Antibody Services for NONO
    Novus Biologicals NONO Antibodies
    Abcam antibodies for NONO
    Cloud-Clone Corp. Antibodies for NONO
    ThermoFisher Antibody for NONO
    LSBio Antibodies in human, mouse, rat for NONO
    antibodies-online antibodies for NONO (49 products) 

    NONO Assay Products:

    EMD Millipore Kits and Assays for the Analysis of NONO
    OriGene Custom Assay Services for NONO
    Browse R&D Systems for biochemical assays
    GenScript Custom Assay Services for NONO
    Browse Enzo Life Sciences for kits & assays
    Cloud-Clone Corp. ELISAs for NONO
    Cloud-Clone Corp. CLIAs for NONO
    Search eBioscience for ELISAs for NONO 
    antibodies-online kits for NONO (7 products) 


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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    HGNC Gene Families:
    RBM: RNA binding motif (RRM) containing

    3 InterPro protein domains:
     IPR012975 NOPS
     IPR000504 RRM_dom
     IPR012677 Nucleotide-bd_a/b_plait

    Graphical View of Domain Structure for InterPro Entry Q15233

    ProtoNet protein and cluster: Q15233

    2 Blocks protein domains:
    IPB000504 RNA-binding region RNP-1 (RNA recognition motif)
    IPB012975 NOPS


    UniProtKB/Swiss-Prot: NONO_HUMAN, Q15233
    Similarity: Contains 2 RRM (RNA recognition motif) domains


    Find genes that share domains with NONO           About GenesLikeMe


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, Vector BioLabs and/or Addgene, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Flow cytometry from eBioscience, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
    About This Section

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    Molecular Function:

         UniProtKB/Swiss-Prot Summary: NONO_HUMAN, Q15233
    Function: DNA- and RNA binding protein, involved in several nuclear processes. Binds the conventional octamer
    sequence in double-stranded DNA. Also binds single-stranded DNA and RNA at a site independent of the duplex site
    (By similarity). Involved in pre-mRNA splicing, probably as a heterodimer with SFPQ. Interacts with U5 snRNA,
    probably by binding to a purine-rich sequence located on the 3' side of U5 snRNA stem 1b. The SFPQ-NONO heteromer
    associated with MATR3 may play a role in nuclear retention of defective RNAs. The SFPQ-NONO heteromer may be
    involved in DNA unwinding by modulating the function of topoisomerase I/TOP1. The SFPQ-NONO heteromer may be
    involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination
    and may stabilize paired DNA ends. In vitro, the complex strongly stimulates DNA end joining, binds directly to
    the DNA substrates and cooperates with the Ku70/G22P1-Ku80/XRCC5 (Ku) dimer to establish a functional preligation
    complex. NONO is involved in transcriptional regulation. The SFPQ-NONO-NR5A1 complex binds to the CYP17 promoter
    and regulates basal and cAMP-dependent transcriptional avtivity. NONO binds to an enhancer element in long
    terminal repeats of endogenous intracisternal A particles (IAPs) and activates transcription (By similarity).
    Together with PSPC1, required for the formation of nuclear paraspeckles

         Gene Ontology (GO): Selected molecular function terms (see all 7):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000166nucleotide binding IEA--
    GO:0003676nucleic acid binding ----
    GO:0003677DNA binding IEA--
    GO:0003723RNA binding ----
    GO:0005515protein binding IPI16148043
         
    Find genes that share ontologies with NONO           About GenesLikeMe


    Phenotypes:
         5 MGI mutant phenotypes (inferred from 4 alleles(MGI details for Nono):
     behavior/neurological  cellular  embryogenesis  homeostasis/metabolism  normal 

    Find genes that share phenotypes with NONO           About GenesLikeMe

    Animal Models:
       inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for NONO
       inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for NONO

       genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for NONO
       genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for NONO

    miRNA
    Products:
        
    miRTarBase miRNAs that target NONO:
    hsa-mir-744-5p (MIRT037550), hsa-mir-149-5p (MIRT045567), hsa-mir-17-5p (MIRT050934), hsa-mir-484 (MIRT041772), hsa-mir-296-3p (MIRT038503), hsa-mir-124-3p (MIRT022721), hsa-mir-615-3p (MIRT040343), hsa-mir-93-5p (MIRT048776), hsa-mir-1301-3p (MIRT035994), hsa-mir-148a-3p (MIRT048042), hsa-mir-148b-3p (MIRT043592), hsa-mir-30c-2-3p (MIRT038697), hsa-mir-92b-3p (MIRT040602), hsa-mir-193b-3p (MIRT041216), hsa-mir-505-5p (MIRT037971)

    Block miRNA regulation of human, mouse, rat NONO using miScript Target Protectors
    Selected qRT-PCR Assays for microRNAs that regulate NONO (see all 27):
    hsa-miR-320a hsa-miR-623 hsa-miR-330-5p hsa-miR-10b hsa-miR-767-5p hsa-miR-4305 hsa-miR-501-3p hsa-miR-650
    SwitchGear 3'UTR luciferase reporter plasmidNONO 3' UTR sequence
    Inhib. RNA
    Products:
        
    OriGene RNAi products in human, mouse, rat for NONO
    Predesigned siRNA for gene silencing in human, mouse, rat NONO

    Gene Editing
    Products:
    DNA2.0 Custom Protein Engineering Service for NONO

    Clone
    Products:
         
    OriGene clones in human, mouse for NONO (see all 24)
    OriGene ORF clones in mouse, rat for NONO
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector (see all 4): NONO (NM_001145409)
    Sino Biological Human cDNA Clone for NONO
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for NONO
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat NONO
    Addgene plasmids for NONO 

    Cell Line
    Products:
         
    GenScript Custom overexpressing Cell Line Services for NONO
    Browse ESI BIO Cell Lines and PureStem Progenitors for NONO 
    In Situ Assay
    Products:
       

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for NONO


    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Subcellular locations from UniProtKB/Swiss-Prot
    NONO_HUMAN, Q15233: Nucleus. Nucleus, nucleolus. Nucleus speckle. Note=Detected in punctate subnuclear
    structures often located adjacent to splicing speckles, called paraspeckles
    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    nucleus5
    plasma membrane3
    extracellular1

    Gene Ontology (GO): 5 cellular component terms:    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634nucleus IDA--
    GO:0005730NOT nucleolus IDA--
    GO:0016363nuclear matrix IDA19874820
    GO:0016607nuclear speck IEA--
    GO:0042382paraspeckles IDA19217333

    Find genes that share ontologies with NONO           About GenesLikeMe


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
    About This Section

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    SuperPaths for NONO About    
    See pathways by source

    SuperPathContained pathways About
    1mRNA Splicing - Major Pathway
    mRNA processing0.44
    2Development NOTCH1 mediated pathway for NF KB activity modulation
    Transcription Sin3 and NuRD in transcription regulation0.35
    3Circadian rhythm pathway
    Circadian rhythm pathway

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways


    1 GeneGo (Thomson Reuters) Pathway for NONO
        Transcription Sin3 and NuRD in transcription regulation

    2 BioSystems Pathways for NONO
        mRNA processing
    Circadian rhythm pathway



        Pathway & Disease-focused RT2 Profiler PCR Arrays including NONO: 
              Housekeeping Genes PCR Array in human mouse rat
              Nuclear Receptors & Coregulators in human mouse rat

    Interactions:

        GeneGlobe Interaction Network for NONO

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    Selected Interacting proteins for NONO (Q152331, 2, 3 ENSP000002760794) via UniProtKB, MINT, STRING, and/or I2D (see all 878)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    SMN1Q166372, 3, ENSP000003700834MINT-7945693 MINT-7947479 I2D: score=2 STRING: ENSP00000370083
    SMN2Q166372, 3, ENSP000003701194MINT-7945693 MINT-7947479 I2D: score=2 STRING: ENSP00000370119
    HDAC1Q135472, 3, ENSP000003626494MINT-7945693 MINT-7947479 I2D: score=1 STRING: ENSP00000362649
    SMARCB1Q128242, 3, ENSP000002631214MINT-7945693 MINT-7947479 I2D: score=1 STRING: ENSP00000263121
    SMARCD1Q96GM52, 3, ENSP000003784144MINT-7945693 MINT-7947479 I2D: score=1 STRING: ENSP00000378414
    About this table

    Gene Ontology (GO): Selected biological process terms (see all 6):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006281DNA repair IEA--
    GO:0006310DNA recombination IEA--
    GO:0006351transcription, DNA-templated IEA--
    GO:0006355regulation of transcription, DNA-templated IEA--
    GO:0006397mRNA processing IEA--

    Find genes that share ontologies with NONO           About GenesLikeMe



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
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    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
      Browse compounds at ApexBio 

    Browse Tocris compounds for NONO

    1 HMDB Compound for NONO    About this table
    CompoundSynonyms CAS #PubMed Ids
    Cyclic AMPCyclic AMP (see all 19)60-92-4--

    2 Novoseek inferred chemical compound relationships for NONO gene    About this table
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    pyrimidine 38.2 1 9756848 (1)
    testosterone 4.74 4 12672026 (2), 17452459 (2)



    Find genes that share compounds with NONO           About GenesLikeMe



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, and/or Addgene, Primers from OriGene, and/or QIAGEN, Flow cytometry from eBioscience )
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    REFSEQ mRNAs for NONO gene (4 alternative transcripts): 
    NM_001145408.1  NM_001145409.1  NM_001145410.1  NM_007363.4  

    Unigene Cluster for NONO:

    Non-POU domain containing, octamer-binding
    Hs.533282  [show with all ESTs]
    Unigene Representative Sequence: NM_001145408
    15 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000276079 ENST00000373856 ENST00000373841 ENST00000474431 ENST00000420903
    ENST00000472185 ENST00000413858 ENST00000486613 ENST00000450092 ENST00000454976
    ENST00000418921 ENST00000471419 ENST00000490044(uc004dzq.3) ENST00000473525
    ENST00000535149(uc004dzo.3 uc004dzn.3 uc004dzp.3 uc011mpv.2)

    miRNA
    Products:
         
    Block miRNA regulation of human, mouse, rat NONO using miScript Target Protectors
    Selected qRT-PCR Assays for microRNAs that regulate NONO (see all 27):
    hsa-miR-320a hsa-miR-623 hsa-miR-330-5p hsa-miR-10b hsa-miR-767-5p hsa-miR-4305 hsa-miR-501-3p hsa-miR-650
    SwitchGear 3'UTR luciferase reporter plasmidNONO 3' UTR sequence
    Inhib. RNA
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    OriGene RNAi products in human, mouse, rat for NONO
    Predesigned siRNA for gene silencing in human, mouse, rat NONO
    Clone
    Products:
         
    OriGene clones in human, mouse for NONO (see all 24)
    OriGene ORF clones in mouse, rat for NONO
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector (see all 4): NONO (NM_001145409)
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for NONO
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat NONO
    Addgene plasmids for NONO 
    Primer
    Products:
        
    OriGene qPCR primer pairs and template standards for NONO
    OriGene qSTAR qPCR primer pairs in human, mouse for NONO
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat NONO
      QuantiTect SYBR Green Assays in human, mouse, rat NONO
      QuantiFast Probe-based Assays in human, mouse, rat NONO

    Additional mRNA sequence: 

    AK291140.1 AK297144.1 BC002364.1 BC003129.1 BC004113.2 BC012141.1 BC025280.1 BC025352.1 
    BC028299.1 BC069616.1 BC069639.1 CR456761.1 L14599.1 U02493.1 U89867.1 

    Selected DOTS entries (see all 101):

    DT.100888721  DT.100888708  DT.92469258  DT.92371968  DT.100832710  DT.100059997  DT.92469289  DT.121290668 
    DT.95364845  DT.100888712  DT.121290569  DT.102841558  DT.121290703  DT.121290627  DT.100888710  DT.121290620 
    DT.92057008  DT.121290608  DT.100888687  DT.121290667  DT.100888697  DT.100059996  DT.100888720  DT.100888718 

    Selected AceView cDNA sequences (see all 2003):

    AU130647 BE205901 BU624728 BQ002318 BU180870 BE732287 BQ877362 BP358971 
    BE296226 BQ229776 BM458164 BG828086 BM828993 BQ230314 BE389963 BM917094 
    BQ004257 BQ643942 BM548704 AU130013 BM910107 CR623522 BE250988 AA593106 

    GeneLoc Exon Structure

    Selected Alternative Splicing Database (ASD) splice patterns (SP) for NONO (see all 8)    About this scheme

    ExUns: 1a · 1b ^ 2 ^ 3 ^ 4a · 4b ^ 5 ^ 6a · 6b ^ 7a · 7b ^ 8a · 8b ^ 9a · 9b ^ 10a · 10b ^ 11 ^ 12 ^ 13 ^ 14 ^ 15a · 15b
    SP1:                                                                                      -           -           -                           
    SP2:                                                                                      -                                                   
    SP3:                                                                                      -           -                                       
    SP4:              -                                                                                                                           
    SP5:                                                                                                                                          


    ECgene alternative splicing isoforms for NONO

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
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    NONO expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: CTGTGCATTT
    NONO Expression
    About this image


    NONO expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database
     selected tissues (see all 22) fully expand
     
     Brain (Nervous System)    fully expand to see all 4 entries
             Cerebral Cortex
     
     Ovary (Reproductive System)    fully expand to see all 2 entries
             Ovarian Mesenchymal Stroma Cells Ovary Interstitium
             Oviduct
     
     Thymus (Hematopoietic System)
             Double Negative 2 Thymocytes Thymus
     
     Testis (Reproductive System)
             Leydig Cells Testis Interstitium
     
     Liver (Hepatobiliary System)
             Hepatocytes Liver Lobule
    NONO Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    NONO Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.533282

    UniProtKB/Swiss-Prot: NONO_HUMAN, Q15233
    Tissue specificity: Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Also found in a
    number of breast tumor cell lines

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    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
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    This gene was present in the common ancestor of animals.

    Orthologs for NONO gene from Selected species (see all 14)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Nono1 , 5 non-POU-domain-containing, octamer binding protein1, 5 91.72(n)1
    98.3(a)1
      X (44.14 cM)5
    536101  NM_001252518.11  NP_001239447.11 
     1014293185 
    chicken
    (Gallus gallus)
    Aves NONO1 non-POU domain containing, octamer-binding 79.22(n)
    87.66(a)
      428701  NM_001031532.1  NP_001026703.1 
    lizard
    (Anolis carolinensis)
    Reptilia NONO6
    non-POU domain containing, octamer-binding
    88(a)
    1 ↔ 1
    GL343599.1(127197-132835)
    African clawed frog
    (Xenopus laevis)
    Amphibia efna2-prov2 non-POU domain containing, octamer-binding 76.1(n)    BC045128.1 
    zebrafish
    (Danio rerio)
    Actinopterygii efna22 ephrin A2 75.33(n)   30218  BC046880.1 
    fruit fly
    (Drosophila melanogaster)
    Insecta nonA3 mRNA splicing RNA binding 43(a)
    (best of 2)
        --
    worm
    (Caenorhabditis elegans)
    Secernentea F25B5.7a3   -- 46(a)   III(6025482-6029983)   --


    ENSEMBL Gene Tree for NONO (if available)
    TreeFam Gene Tree for NONO (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
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    Paralogs for NONO gene
    PSPC12  SFPQ2  
    3 SIMAP similar genes for NONO using alignment to 6 protein entries:     NONO_HUMAN (see all proteins):
    SFPQ    PSPC1    RBM4B

    Find genes that share paralogs with NONO           About GenesLikeMe


    3 Pseudogenes.org Pseudogenes for NONO
    PGOHUM00000248305 PGOHUM00000248919 PGOHUM00000240055


    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
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    Selected SNPs for NONO (see all 238)    About this table                                 

    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr X posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1490019371,2
    --64317338(+) AATTAC/TTGGTT 4 -- us2k10--------
    rs1830867981,2
    --64317737(+) AGCTGA/TGATCG 4 -- us2k10--------
    rs735499411,2
    C,F--70501388(+) AACCCC/ACCGTG 4 -- us2k12Minor allele frequency- A:0.33WA CSA 3
    rs1930461281,2
    --70501577(+) GAAACA/GCAAGA 4 -- us2k10--------
    rs1846219781,2
    --70501617(+) TGAATG/TGGGGG 4 -- us2k10--------
    rs1893867431,2
    --70501682(+) GCGCAA/GTCTTG 4 -- us2k10--------
    rs1384882831,2
    --70502437(+) TAGATC/TATTGT 4 -- us2k10--------
    rs1413285851,2
    --70502466(+) GCCCAC/TTGGGA 4 -- us2k10--------
    rs1451107861,2
    --70502564(+) GGGGCA/GGGGCG 4 -- us2k10--------
    rs1926243671,2
    --70502828(+) CTGTAA/CCCATG 4 -- us2k10--------

    HapMap Linkage Disequilibrium report for NONO (70503042 - 70521018 bp)

    Structural Variations
         Database of Genomic Variants (DGV) 3 variations for NONO:    About this table    
    Variant IDTypeSubtypePubMed ID
    nsv508781CNV Insertion20534489
    esv33082CNV Loss17666407
    esv1584074OTHER Inversion17803354

    Site Specific Mutation Identification with PCR Assays
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    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
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    OMIM gene information: 300084    OMIM disorders: --

    UniProtKB/Swiss-Prot: NONO_HUMAN, Q15233
  • Note=A chromosomal aberration involving NONO may be a cause of papillary renal cell carcinoma (PRCC).
    Translocation t(X;X)(p11.2;q13.1) with TFE3

  • 3 diseases for NONO:    
    About MalaCards
    renal cell carcinoma, papillary, 1    papillary renal cell carcinoma    renal cell carcinoma


    Find genes that share disorders with NONO           About GenesLikeMe

    Human Genome Epidemiology (HuGE) Navigator: NONO (1 document)

    Export disorders for NONO gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
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    PubMed articles for NONO gene, integrated from 10 sources (see all 150):
    (articles sorted by number of sources associating them with NONO)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Purification and cDNA cloning of HeLa cell p54nrb, a nuclear protein with two RNA recognition motifs and extensive homology to human splicing factor PSF and Drosophila NONA/BJ6. (PubMed id 8371983)1, 2, 3, 9 Dong B.... Krainer A.R. (Nucleic Acids Res. 1993)
    2. Loss of expression of a 55 kDa nuclear protein (nmt55) in estrogen receptor-negative human breast cancer. (PubMed id 9360842)1, 2, 3 Traish A.M.... Moreland R.B. (Diagn. Mol. Pathol. 1997)
    3. Fusion of splicing factor genes PSF and NonO (p54nrb) to the TFE3 gene in papillary renal cell carcinoma. (PubMed id 9393982)1, 2, 9 Clark J.... Cooper C.S. (Oncogene 1997)
    4. P54nrb forms a heterodimer with PSP1 that localizes to paraspeckles in an RNA-dependent manner. (PubMed id 16148043)1, 2, 9 Fox A.H.... Lamond A.I. (Mol. Biol. Cell 2005)
    5. The RNA-splicing factor PSF/p54 controls DNA-topoisomerase I activity by a direct interaction. (PubMed id 9756848)1, 2, 9 Straub T.... Boege F. (J. Biol. Chem. 1998)
    6. Proteomic identification of a PSF/p54nrb heterodimer as RNF43 oncoprotein-interacting proteins. (PubMed id 18655028)1, 2, 9 Miyamoto K.... Sugiura T. (Proteomics 2008)
    7. Identification of the polypyrimidine tract binding protein-associated splicing factor.p54(nrb) complex as a candidate DNA double-strand break rejoining factor. (PubMed id 15590677)1, 2, 9 Bladen C.L.... Dynan W.S. (J. Biol. Chem. 2005)
    8. Structure of the heterodimer of human NONO and paraspeckle protein component 1 and analysis of its role in subnuclear body formation. (PubMed id 22416126)1, 2 Passon D.M.... Bond C.S. (Proc. Natl. Acad. Sci. U.S.A. 2012)
    9. A probability-based approach for high-throughput protein phosphorylation analysis and site localization. (PubMed id 16964243)1, 2 Beausoleil S.A.... Gygi S.P. (Nat. Biotechnol. 2006)
    10. Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. (PubMed id 17081983)1, 2 Olsen J.V....Mann M. (Cell 2006)

    (in PubMed, OMIM, and NCBI Bookshelf)
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     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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    Entrez Gene: 4841 HGNC: 7871 AceView: NONO Ensembl:ENSG00000147140 euGenes: HUgn4841
    ECgene: NONO H-InvDB: NONO

    (According to HUGE)
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      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
    About This Section

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    NameDescription
    PharmGKB entry for NONO Pharmacogenomics, SNPs, Pathways
    ATLAS Chromosomes in Cancer entry for NONO Genetics and Cytogenetics in Oncology and Haematology

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
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    Patent Information for NONO gene:
    Search GeneIP for patents involving NONO

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, eBioscience, antibodies-online, and/or others, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Addgene, Cell lines from GenScript, and ESI BIO, Flow cytometery from eBioscience, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
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