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Aliases &
Descriptions
for NONO
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc
, and/or 7Ensembl,
8miRBase)
About This Section
|
| Aliases |
|---|
| NMT55 1, 2, 3 | | NRB54 1, 2, 3, 5 | | OTTHUMP00000023506 2 | | OTTHUMP00000023507 2 | | OTTHUMP00000023508 2 | | P54 1, 2 | | P54NRB 1, 2 | | p54(nrb) 3 | | p54nrb 3 |
| | | Descriptions |
|---|
| 54 kDa nuclear RNA- and DNA-binding protein 2, 3 | | 55 kDa nuclear protein 3 | | DNA-binding p52/p100 complex, 52 kDa subunit 3 | | NonO protein 3 | | non-POU domain containing, octamer-binding 2 | non-POU domain-containing octamer (ATGCAAAT) binding
protein 2 | | non-POU-domain-containing, octamer-binding 1 |
|
| |
Search outside databases for aliases for NONO genePrevious GC identifers: GC0XP066677 GC16M070209 GC16M069524 GC16M069565 GC0XP069370 GC0XP070286 |
Summaries
for NONO(According to Entrez Gene,
Wikipedia's
Gene Wiki,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
| EntrezGene summary for NONO:
This gene encodes an RNA-binding protein which plays various roles in the nucleus, including
transcriptional regulation and RNA splicing. A rearrangement between this gene and the
transcription factor E3 gene has been observed in papillary renal cell carcinoma. Alternatively
spliced transcript variants have been described. Pseudogenes exist on Chromosomes 2 and 16.
[provided by RefSeq]
UniProtKB/Swiss-Prot: NONO_HUMAN, Q15233Function: DNA- and RNA binding protein, involved in several nuclear processes. Binds the
conventional octamer sequence in double stranded DNA. Also binds single-stranded DNA and RNA at a
site independent of the duplex site (By similarity). Involved in pre-mRNA splicing, probably as an
heterodimer with SFPQ. Interacts with U5 snRNA, probably by binding to a purine-rich sequence
located on the 3' side of U5 snRNA stem 1b. The SFPQ-NONO heteromer associated with MATR3 may play
a role in nuclear retention of defective RNAs. The SFPQ-NONO heteromer may be involved in DNA
unwinding by modulating the function of topoisomerase I/TOP1. The SFPQ-NONO heteromer may be
involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J
recombination and may stabilize paired DNA ends. In vitro, the complex strongly stimulates DNA end
joining, binds directly to the DNA substrates and cooperates with the Ku70/G22P1-Ku80/XRCC5 (Ku)
dimer to establish a functional preligation complex. Nono is involved in transcriptional
regulation. The SFPQ-NONO-NR5A1/SF-1 complex binds to the CYP17 promoter and regulates basal and
cAMP-dependent transcriptional avtivity. NONO binds to an enhancer element in long terminal
repeats of endogenous intracisternal A particles (IAPs) and activates transcription (By
similarity)Gene Wiki entry for NONO |
Genomic Location
for NONO
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 36),
and/or miRBase,
Genomic Views according to
UCSC and
Ensembl,
Transcription factor binding sites according to
SABiosciences)
About This Section
| Genomic View: UCSC Golden Path with GeneCards custom track
 Transcription factor binding sites upstream to the NONO gene 
Entrez Gene cytogenetic band: Xq13.1 Ensembl cytogenetic band: Xq13.1 HGNC cytogenetic band: Xq13.1NONO Gene in genomic location: bands according to Ensembl, locations according to
 (and/or Entrez Gene and/or Ensembl if different)
GeneLoc gene densities for chromosome X GeneLoc Exon Structure GeneLoc location for GC0XP070420:
(about GC identifiers)
Start:
|
70,420,165 bp from pter |
End:
|
70,437,743 bp from pter |
Size:
|
17,579 bases |
Orientation:
|
plus strand |
RefSeq DNA sequence:- NC_000023.9 NT_011669.16
| Proteins
for NONO
(According to
1UniProtKB,
and/or Ensembl,
Phosphorylation sites according to 2Phosphosite,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from Invitrogen,
Millipore,
Sigma-Aldrich,
R&D Systems,
Enzo Life Sciences,
Abnova,
OriGene and/or,
Abcam,
Biochemical Assays by
Invitrogen,
Millipore,
R&D Systems,
Cell Signaling Technology, and/or
Enzo Life Sciences,
Ontologies according to Gene
Ontology Consortium 01 Apr 2009 and
Entrez Gene,
Antibodies by Invitrogen,
Millipore,
Sigma-Aldrich,
R&D Systems,
Cell Signaling Technology,
Abcam,
Abnova, and/or
Novus Biologicals)
About This Section
| UniProtKB/Swiss-Prot: NONO_HUMAN, Q15233 (See
protein sequence)Recommended Name: Non-POU domain-containing octamer-binding protein Size: 471 amino acids; 54232 Da
Subunit: Monomer and component of the SFPQ-NONO complex, which is probably a heterotetramer of two
52 kDa (NONO) and two 100 kDa (SFPQ) subunits. NONO is a component of spliceosome and U5.4/6 snRNP
complexes. Interacts with PSPC1 and SNRPA/U1A. Part of complex consisting of SFPQ, NONO and MATR3.
Part of a complex consisting of SFPQ, NONO and NR5A1/SF-1. Part of a complex consisting of SFPQ,
NONO and TOP1. Interacts with SPI1 (By similarity). Interacts with RNF43
Subcellular location: Nucleus
Secondary accessions: O00201 P30807 Q12786 Q9BQC5Post-translational modifications:
The N-terminus is blocked1
View phosphorylation sites using PhosphoSite2
REFSEQ proteins (4 alternative transcripts):
NP_001138880.1 NP_001138881.1 NP_001138882.1 NP_031389.3
ENSEMBL proteins: ENSP00000276079 ENSP00000362963 ENSP00000362947
Human Recombinant Proteins  OriGene Purified Recombinant Human Protein: NONO
1 Gene Ontology (GO) cellular component term (links to tree view): About this table
Antibodies for NONO:
Assays for NONO: | Protein
Domains/
Families
for NONO(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
| - Graphical View of Domain Structure for InterPro Entry Q15233
ProtoNet protein and cluster: Q15233 2 Blocks protein families: IPB000504 RNA-binding region RNP-1 (RNA recognition motif) IPB012975 NOPS
UniProtKB/Swiss-Prot: NONO_HUMAN, Q15233Similarity: Contains 2 RRM (RNA recognition motif) domains | Gene Function
for NONO
(According to MGI Jun 06 2009, UniProtKB,
IUBMB,and/or Genatlas,
shRNA from
OriGene,
Sigma-Aldrich, RNAi from
Sigma-Aldrich,
RNAi Products,
Clones, and
Q-PCR Products
from Invitrogen,
Millipore,
OriGene, and/or
Abnova,
siRNAs from
Applied Biosystems,
SYBR primers from OriGene,
Cell-based Assays from Millipore,
Ontologies according to Gene Ontology Consortium 01 Apr 2009 via
Entrez Gene.)
About This Section
| OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 4): NM_007363
 Applied Biosystems Silencer® siRNAs for NONO
 Sigma-Aldrich siRNA for NONO
Sigma-Aldrich shRNA Panels and shRNA for NONO
Explore Sigma-Aldrich super-pooled esiRNAs
OriGene GFP tagged cDNA clones in CMV expression vector (see all 4): NM_001145408
Myc/DDK tagged cDNA clones in CMV expression vector (see all 4): NM_001145408
untagged cDNA clones in CMV expression vector (see all 6): NM_001145408
Primers:  Browse
Quantitative PCR Central at Invitrogen for Q-PCR LUX™ Primers
OriGene genome-wide validated SYBR primer pairs: NM_001145409
UniProtKB/Swiss-Prot: NONO_HUMAN, Q15233Function: DNA- and RNA binding protein, involved in several nuclear processes. Binds the
conventional octamer sequence in double stranded DNA. Also binds single-stranded DNA and RNA at a
site independent of the duplex site (By similarity). Involved in pre-mRNA splicing, probably as an
heterodimer with SFPQ. Interacts with U5 snRNA, probably by binding to a purine-rich sequence
located on the 3' side of U5 snRNA stem 1b. The SFPQ-NONO heteromer associated with MATR3 may play
a role in nuclear retention of defective RNAs. The SFPQ-NONO heteromer may be involved in DNA
unwinding by modulating the function of topoisomerase I/TOP1. The SFPQ-NONO heteromer may be
involved in DNA nonhomologous end joining (NHEJ) required for double-strand break repair and V(D)J
recombination and may stabilize paired DNA ends. In vitro, the complex strongly stimulates DNA end
joining, binds directly to the DNA substrates and cooperates with the Ku70/G22P1-Ku80/XRCC5 (Ku)
dimer to establish a functional preligation complex. Nono is involved in transcriptional
regulation. The SFPQ-NONO-NR5A1/SF-1 complex binds to the CYP17 promoter and regulates basal and
cAMP-dependent transcriptional avtivity. NONO binds to an enhancer element in long terminal
repeats of endogenous intracisternal A particles (IAPs) and activates transcription (By
similarity)4 Gene Ontology (GO) molecular function terms (links to tree view): About this table | Pathways & Interactions
for NONO
(Pathways according to Invitrogen
(maps by GeneGo),
Millipore,
Cell Signaling Technology,
Sigma-Aldrich,
KEGG
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Proteins Network according to
SABiosciences,
Interactions according to 1UniProtKB,
2MINT, and/or
3STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Apr 2009 via
Entrez Gene.)
About This Section
|
Gene Network CentralTM Interacting Genes and Proteins Network for NONO
5/26 Interacting proteins for NONO (ENSP000002760793 Q152331, 2) via UniProtKB, MINT, and/or STRING (see all 26
)About this table
5/7 Gene Ontology (GO) biological process terms (links to tree view) (see all 7
): About this table
|
Drugs & Compounds
for NONO(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
Sigma-Aldrich, Tocris Bioscience, and/or
Novoseek and Drugs according to
Enzo Life Sciences and/or
PharmGKB)
About This Section
|
 Browse Tocris compounds for NONO
1  Novoseek chemical compound relationship for NONO gene
| Compound |
Score |
Articles |
PubMed IDs for Articles with Shared Sentences (# sentences) |
| pyrimidine |
41.17 |
1 |
9756848 (1) |
About this table
|
Transcripts
for NONO(GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 219 Homo sapiens; Jun 2 2009) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
non coding RNAs according to
RNAdb,
ESTs according to GeneTide,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from Invitrogen,
Millipore, and/or
Abnova,
siRNAs from Applied Biosystems,
Sigma-Aldrich,
shRNA from
Sigma-Aldrich,
OriGene,
Tagged/untagged cDNA clones from
OriGene,
Expression Assays from Applied Biosystems)
About This Section
| OriGene 29mer shRNA kit in GFP-retroviral vectors (see all 4): NM_007363
Sigma-Aldrich siRNA for NONO
Sigma-Aldrich shRNA Panels and shRNA for NONO
Explore Sigma-Aldrich super-pooled esiRNAs
Applied Biosystems Silencer® siRNAs: NM_007363 REFSEQ mRNAs for NONO gene (4 alternative transcripts): NM_001145408.1 NM_001145409.1 NM_001145410.1 NM_007363.4
Applied Biosystems TaqMan ® Gene Expression Assays: NM_007363
OriGene GFP tagged cDNA clones in CMV expression vector (see all 4): NM_001145408
Myc/DDK tagged cDNA clones in CMV expression vector (see all 4): NM_001145408
untagged cDNA clones in CMV expression vector (see all 6): NM_001145408
Additional cDNA sequence: L32558.1 24/105 DOTS entries (see all 105
): DT.100888721 DT.100888708 DT.92469258 DT.92371968 DT.100832710 DT.100059997 DT.95364845 DT.100888712 DT.92469289 DT.121290668 DT.121290569 DT.102841558 DT.121290703 DT.121290627 DT.100888710 DT.121290620 DT.92057008 DT.121290608 DT.121290667 DT.100888687 DT.100888720 DT.100888697 DT.100059996 DT.100888718 24/2003 AceView cDNA sequences (see all 2003
):AI092020 BG763553 BQ890178 AL046552 BU790410 BE336846 AI754076 BU526800 BG107421 BP366446 BM480307 BE250856 BQ053613 AI859601 AA380359 AW238821 AU132245 BQ070004 BP346236 AA456510 BE296904 BU159741 BU178126 AU131334
 highest scoring ESTs for NONO:U02493 AA010871 AA022901 AA022911 AA056465 AA065336 AA065337 AA069827 AA075719 AA090066 Unigene Clusters for NONO: Non-POU domain containing, octamer-binding Hs.533282 [show with all ESTs], Hs.700344 [show with all ESTs]Unigene Representative Sequences: NM_001145408, BU940180
GeneLoc Exon Structure
5/8 Alternative Splicing Database (ASD) splice patterns (SP) for NONO (see all 8
)
| ExUns: | 1a | · | 1b | ^ | 2 | ^ | 3 | ^ | 4a | · | 4b | ^ | 5 | ^ | 6a | · | 6b | ^ | 7a | · | 7b | ^ | 8a | · | 8b | ^ | 9a | · | 9b | ^ | 10a | · | 10b | ^ | 11 | ^ | 12 | ^ | 13 | ^ | 14 | ^ | 15a | · | 15b |
|---|
|
| SP1: | | | | | | | | | | | | | | | | | | | | | | | | | | | | | - | | | | - | | | | - | | | | | | | | | | |
| SP2: | | | | | | | | | | | | | | | | | | | | | | | | | | | | | - | | | | | | | | | | | | | | | | | | |
| SP3: | | | | | | | | | | | | | | | | | | | | | | | | | | | | | - | | | | - | | | | | | | | | | | | | | |
| SP4: | | | | | - | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | |
| SP5: | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | |
About this scheme
ECgene alternative splicing isoforms for NONO
3 Ensembl transcripts including schematic representations: ENST00000276079
ENST00000373856
ENST00000373841
|
Expression
for NONO
(Experimental results according to
1GeneNote
and GNF BioGPS,
probe sets-to-genes annotations according to
2GeneAnnot ,
3GeneTide ,
Sets of similar genes according to GeneDecks,
Electronic Northern calculations according to data from
UniGene (Build 219 Homo sapiens),
SAGE tags according to
CGAP,
plus additional links to
SOURCE, and/or
GNF
BioGPS, and/or
EXPOLDB, and/or
UniProtKB,
Expression Assays from
Applied Biosystems
)
About This Section
| NONO expression in normal and diseased human tissues
Applied Biosystems TaqMan ® Gene Expression Assays for NONO
1 / 2 / 3 7 probe-sets matching NONO gene Data from
(Publications) and GNF BioGPS
About these images
About these images
CGAP SAGE TAG: CTGTGCATTT
SOURCE GeneReport for Unigene clusters: Hs.533282 Hs.700344
Expression variation in blood from EXPOLDB for NONO UniProtKB/Swiss-Prot: NONO_HUMAN, Q15233Tissue specificity: Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Also
found in a number of breast tumor cell lines |
Orthologs
for NONO
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
and/or
5MGI Jun 06 2009,
with possible further links to
Flybase
and/or
WormBase,
Gene Trees according to Ensembl)
About This Section
|
Orthologs for NONO gene from 5/11 species (see all 11
)
About this table Species with no ortholog for NONO
ENSEMBL Gene Tree for NONO | Paralogs
for NONO(Paralogs according to 1HomoloGene
and 2Ensembl, Pseudogenes according to 3Pseudogene.org)
About This Section
| Paralogs for NONO gene
- PSPC12 SFPQ2
|
SNPs/Variants
for NONO(According to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE, and
UniProtKB,
Linkage Disequilibrium by HapMap,
Genotyping Reagents from
Applied Biosystems)
About This Section
|
HapMap Linkage Disequilibrium images for NONO (up to first 250kb)
|
Disorders & Mutations
for NONO
(in which this Gene is Involved, According to
OMIM, UniProtKB,
PharmGKB,
Genatlas, GeneTests,
Blood group antigen gene mutations by BGMUT,
HGMD, GAD,
HuGE Navigator,
BCGD,
and/or TGDB.)
About This Section
|
OMIM: 300084
UniProtKB/Swiss-Prot: NONO_HUMAN, Q15233
A chromosomal aberration involving NONO may be a cause of papillary renal cell carcinoma
(PRCC). Translocation t(X;X)(p11.2;q13.1) with TFE3 |
Medical News
for NONO(Possibly Related Articles in
Doctor's Guide)
About This Section
| -- |
Publications
for NONO (in
PubMed.
Associations of this gene to articles via
1Novoseek,
2HGNC,
3Entrez Gene,
4UniProtKB/Swiss-Prot,
5UniProtKB/TrEMBL,
6GAD, and/or
7PharmGKB)
About This Section
| 10/63 PubMed articles for NONO gene (see all 63
):- Purification and cDNA cloning of HeLa cell p54nrb, a nuclear protein with two RNA recognition motifs and extensive homology to human splicing factor PSF and Drosophila NONA/BJ6. (PubMed id 8371983)1, 2, 3, 4 Dong B.... Krainer A.R. (1993)
- Loss of expression of a 55 kDa nuclear protein (nmt55) in estrogen receptor-negative human breast cancer. (PubMed id 9360842)2, 3, 4 Traish A.M....Moreland R.B. (1997)
- Fusion of splicing factor genes PSF and NonO (p54nrb) to the TFE3 gene in papillary renal cell carcinoma. (PubMed id 9393982)1, 3, 4 Clark J.... Cooper C.S. (1997)
- P54nrb forms a heterodimer with PSP1 that localizes to paraspeckles in an RNA-dependent manner. (PubMed id 16148043)1, 3, 4 Fox A.H.... Lamond A.I. (2005)
- The RNA-splicing factor PSF/p54 controls DNA-topoisomerase I activity by a direct interaction. (PubMed id 9756848)1, 3, 4 Straub T.... Boege F. (1998)
- Proteomic identification of a PSF/p54nrb heterodimer as RNF43 oncoprotein-interacting proteins. (PubMed id 18655028)1, 3, 4 Miyamoto K....Sugiura T. (2008)
- Identification of the polypyrimidine tract binding protein-associated splicing factor.p54(nrb) complex as a candidate DNA double-strand break rejoining factor. (PubMed id 15590677)1, 3, 4 Bladen C.L.... Dynan W.S. (2005)
- A probability-based approach for high-throughput protein phosphorylation analysis and site localization. (PubMed id 16964243)3, 4 Beausoleil S.A.... Gygi S.P. (2006)
- Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. (PubMed id 17081983)3, 4 Olsen J.V....Mann M. (2006)
- The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)3, 4 Gerhard D.S....Malek J. (2004)
|
Search for NONO
(in PubMed,
OMIM, and NCBI Bookshelf)
About This Section
|
|
Genome Databases showing NONO
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
and/or
H-InvDB)
About This Section
|
| Other Databases showing NONO
(According to HUGE)
About This Section
| -- |
Specialized Databases showing NONO(According to ATLAS, HORDE, IMGT, MTDB, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section
|
| About This Section
| --
| Services
for NONO(Reagents available from Applied Biosystems, Antibodies and assays by Cell
Signaling Technology, Abcam, Novus Biologicals,
Sigma-Aldrich, R&D Systems, Millipore, Abnova, and/or Invitrogen, Clones available from OriGene,and/or Invitrogen, Drugs and/or compounds by Sigma-Aldrich,
Enzo Life Sciences, and/or Tocris Bioscience)
About This Section
| 
Products for NONO:
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