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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

NLK Gene

protein-coding   GIFtS: 64
GCID: GC17P026369

Nemo-Like Kinase

(Previous name: nemo like kinase)
  See related diseases
at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
About This Section

Aliases
Nemo-Like Kinase1 2 3     LAK13
EC 2.7.11.243 8     Protein LAK13
Nemo Like Kinase1     EC 2.7.118
Serine/Threonine-Protein Kinase NLK2     

External Ids:    HGNC: 298581   Entrez Gene: 517012   Ensembl: ENSG000000870957   OMIM: 6094765   UniProtKB: Q9UBE83   

Export aliases for NLK gene to outside databases

Previous GC identifers: GC17P026225 GC17P028269 GC17P026515 GC17P026514 GC17P023393 GC17P022577


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

GeneCards Summary for NLK Gene: 
NLK (nemo-like kinase) is a protein-coding gene. Diseases associated with NLK include neurofibromatosis, and colon cancer, and among its related super-pathways are Wnt signaling pathway and MAPK signaling pathway. GO annotations related to this gene include protein kinase activity and identical protein binding. An important paralog of this gene is MAPK4.

UniProtKB/Swiss-Prot: NLK_HUMAN, Q9UBE8
Function: Serine/threonine-protein kinase that regulates a number of transcription factors with key roles in cell
fate determination. Positive effector of the non-canonical Wnt signaling pathway, acting downstream of WNT5A,
MAP3K7/TAK1 and HIPK2. Activation of this pathway causes binding to and phosphorylation of the histone
methyltransferase SETDB1. The NLK-SETDB1 complex subsequently interacts with PPARG, leading to methylation of
PPARG target promoters at histone H3K9 and transcriptional silencing. The resulting loss of PPARG target gene
transcription inhibits adipogenesis and promotes osteoblastogenesis in mesenchymal stem cells (MSCs). Negative
regulator of the canonical Wnt/beta-catenin signaling pathway. Binds to and phosphorylates TCF7L2/TCF4 and LEF1,
promoting the dissociation of the TCF7L2/LEF1/beta-catenin complex from DNA, as well as the ubiquitination and
subsequent proteolysis of LEF1. Together these effects inhibit the transcriptional activation of canonical
Wnt/beta-catenin target genes. Negative regulator of the Notch signaling pathway. Binds to and phosphorylates
NOTCH1, thereby preventing the formation of a transcriptionally active ternary complex of NOTCH1, RBPJ/RBPSUH and
MAML1. Negative regulator of the MYB family of transcription factors. Phosphorylation of MYB leads to its
subsequent proteolysis while phosphorylation of MYBL1 and MYBL2 inhibits their interaction with the coactivator
CREBBP. Other transcription factors may also be inhibited by direct phosphorylation of CREBBP itself. Acts
downstream of IL6 and MAP3K7/TAK1 to phosphorylate STAT3, which is in turn required for activation of NLK by
MAP3K7/TAK1

Gene Wiki entry for NLK Gene


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 73), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000017.10  NT_010799.15  NC_018928.2  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the NLK gene promoter:
         E2F-4   E2F-3a   E2F-5   p300   E2F-2   PPAR-gamma1   E2F   PPAR-gamma2   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmids (see all 2): NLK promoter sequence
   Search SABiosciences Chromatin IP Primers for NLK

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat NLK


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 17q11.2   Ensembl cytogenetic band:  17q11.2   HGNC cytogenetic band: 17q11.2

NLK Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
NLK gene location

GeneLoc information about chromosome 17         GeneLoc Exon Structure

GeneLoc location for GC17P026369:  view genomic region     (about GC identifiers)

Start:
26,368,763 bp from pter      End:
26,523,407 bp from pter
Size:
154,645 bases      Orientation:
plus strand

(According to UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MAXQB RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Cloud-Clone Corp.,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Cloud-Clone Corp., Ontologies according to Gene Ontology Consortium 01 Oct 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, and/or Cloud-Clone Corp.)
About This Section

UniProtKB/Swiss-Prot: NLK_HUMAN, Q9UBE8 (See protein sequence)
Recommended Name: Serine/threonine-protein kinase NLK  
Size: 527 amino acids; 58283 Da
Cofactor: Magnesium (By similarity)
Subunit: Homodimer. Homodimerization is required for intermolecular autophosphorylation, kinase activation and
nuclear localization (By similarity). May interact with components of cullin-RING-based SCF (SKP1-CUL1-F-box
protein) E3 ubiquitin-protein ligase complexes (By similarity). Interacts with LEF1, MEF2A, MYBL1 and MYBL2 (By
similarity). Interacts with the upstream activating kinases HIPK2 and MAP3K7/TAK1. Interaction with MAP3K7/TAK1
seems to be indirect, and may be mediated by other proteins such as STAT3, TAB1 and TAB2. Interacts with and
phosphorylates a number of transcription factors including FOXO1, FOXO3, FOXO4, MYB, NOTCH1 and TCF7L2/TCF4.
Interacts with DAPK3/ZIPK, and this interaction may disrupt interaction with transcription factors such as
TCF7L2/TCF4. Forms a transcriptional repressor complex with CHD7, PPARG and SETDB1. Interacts with RNF138/NARF
Subcellular location: Nucleus (By similarity). Cytoplasm (By similarity). Note=Predominantly nuclear. A smaller
fraction is cytoplasmic (By similarity)
Sequence caution: Sequence=AAD56013.1; Type=Erroneous initiation; Note=Translation N-terminally extended;
Sequence=AAF04857.1; Type=Erroneous initiation; Note=Translation N-terminally extended; Sequence=ABC40748.1;
Type=Erroneous initiation; Note=Translation N-terminally extended; Sequence=BAG37718.1; Type=Erroneous
initiation; Note=Translation N-terminally extended;
Secondary accessions: B2RCX1 Q2PNI9 Q6P2A3

Explore the universe of human proteins at neXtProt for NLK: NX_Q9UBE8

Explore proteomics data for NLK at MOPED 

Post-translational modifications:

  • UniProtKB: Phosphorylated on Thr-298. Intermolecular autophosphorylation on Thr-298 activates the enzyme
  • View modification sites using PhosphoSitePlus
  • View neXtProt modification sites for NX_Q9UBE8

  • 4/14 DME Specific Peptides for NLK (Q9UBE8) (see all 14)
     FDPSKRI  TDLLGTP  LKICDFG  HRDIKPGN 

    NLK Protein expression data from MOPED1, PaxDb2 and MAXQB3 :    About this image 

    NLK Protein Expression
    REFSEQ proteins: NP_057315.3  
    ENSEMBL proteins: 
     ENSP00000384625   ENSP00000464656   ENSP00000433117  

    Human Recombinant Protein Products for NLK: 
    EMD Millipore Purified and/or Recombinant NLK Protein
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    OriGene Purified Protein for NLK
    OriGene Protein Over-expression Lysate for NLK
    OriGene MassSpec for NLK 
    OriGene Custom Protein Services for NLK
    GenScript Custom Purified and Recombinant Proteins Services for NLK
    Novus Biologicals NLK Proteins
    Novus Biologicals NLK Lysate
    Sino Biological Recombinant Protein for NLK
    Sino Biological Cell Lysate for NLK 
    Browse ProSpec Recombinant Proteins
    Cloud-Clone Corp. Proteins for NLK 

    Gene Ontology (GO): 2 cellular component terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634nucleus ISS--
    GO:0005737cytoplasm IEA--

    NLK for ontologies           About GeneDecksing



    NLK Antibody Products: 
    EMD Millipore Mono- and Polyclonal Antibodies for the study of NLK
    Browse R&D Systems for Antibodies
    OriGene Antibodies for NLK
    OriGene Custom Antibody Services for NLK
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    Novus Biologicals NLK Antibodies
    Abcam antibodies for NLK
    Cloud-Clone Corp. Antibodies for NLK 
    ThermoFisher Antibody for NLK
    LSBio Antibodies in human, mouse, rat for NLK 

    Assay Products for NLK: 
    Browse Kits and Assays available from EMD Millipore
    OriGene Custom Assay Services for NLK
    Browse R&D Systems for biochemical assays
    GenScript Custom Assay Services for NLK
    Browse Enzo Life Sciences for kits & assays
    Cloud-Clone Corp. ELISAs for NLK 
    Cloud-Clone Corp. CLIAs for NLK


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section
    IUPHAR Guide to PHARMACOLOGY protein family classification: nemo-like kinase 
    nmo subfamily

    5/6 InterPro protein domains (see all 6):
     IPR017441 Protein_kinase_ATP_BS
     IPR002290 Ser/Thr_dual-sp_kinase_dom
     IPR011009 Kinase-like_dom
     IPR008271 Ser/Thr_kinase_AS
     IPR003527 MAP_kinase_CS

    Graphical View of Domain Structure for InterPro Entry Q9UBE8

    ProtoNet protein and cluster: Q9UBE8

    1 Blocks protein domain: IPB003527 MAP kinase

    UniProtKB/Swiss-Prot: NLK_HUMAN, Q9UBE8
    Domain: Contains a TQE activation loop motif in which autophosphorylation of the threonine residue (Thr-298) is
    sufficient for kinase activation. This mode of activation contrasts with that of classical MAP kinases, which
    contain a TXY activation loop motif in which phosphorylation of both the threonine and tyrosine residues is
    required for kinase activation
    Similarity: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily
    Similarity: Contains 1 protein kinase domain


    NLK for domains           About GeneDecksing


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase, shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Sirion Biotech, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, Vector BioLabs, and Sirion Biotech, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Oct 2013 via Entrez Gene.)
    About This Section

    Molecular Function:

         UniProtKB/Swiss-Prot Summary: NLK_HUMAN, Q9UBE8
    Function: Serine/threonine-protein kinase that regulates a number of transcription factors with key roles in cell
    fate determination. Positive effector of the non-canonical Wnt signaling pathway, acting downstream of WNT5A,
    MAP3K7/TAK1 and HIPK2. Activation of this pathway causes binding to and phosphorylation of the histone
    methyltransferase SETDB1. The NLK-SETDB1 complex subsequently interacts with PPARG, leading to methylation of
    PPARG target promoters at histone H3K9 and transcriptional silencing. The resulting loss of PPARG target gene
    transcription inhibits adipogenesis and promotes osteoblastogenesis in mesenchymal stem cells (MSCs). Negative
    regulator of the canonical Wnt/beta-catenin signaling pathway. Binds to and phosphorylates TCF7L2/TCF4 and LEF1,
    promoting the dissociation of the TCF7L2/LEF1/beta-catenin complex from DNA, as well as the ubiquitination and
    subsequent proteolysis of LEF1. Together these effects inhibit the transcriptional activation of canonical
    Wnt/beta-catenin target genes. Negative regulator of the Notch signaling pathway. Binds to and phosphorylates
    NOTCH1, thereby preventing the formation of a transcriptionally active ternary complex of NOTCH1, RBPJ/RBPSUH and
    MAML1. Negative regulator of the MYB family of transcription factors. Phosphorylation of MYB leads to its
    subsequent proteolysis while phosphorylation of MYBL1 and MYBL2 inhibits their interaction with the coactivator
    CREBBP. Other transcription factors may also be inhibited by direct phosphorylation of CREBBP itself. Acts
    downstream of IL6 and MAP3K7/TAK1 to phosphorylate STAT3, which is in turn required for activation of NLK by
    MAP3K7/TAK1
    Catalytic activity: ATP + a protein = ADP + a phosphoprotein
    Enzyme regulation: Activated by dimerization and subsequent intermolecular autophosphorylation on Thr-298 (By
    similarity). Activated by the non-canonical Wnt signaling pathway, in which WNT5A treatment leads to activation
    of MAP3K7/TAK1 and HIPK2, which subsequently phosphorylates and activates this protein. Other cytokines such as
    IL6 may also activate this regulatory circuit

         Enzyme Numbers (IUBMB): EC 2.7.11.241 2 EC 2.7.112

         Gene Ontology (GO): 5/12 molecular function terms (GO ID links to tree view) (see all 12):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000287magnesium ion binding ISS--
    GO:0004672protein kinase activity TAS10863097
    GO:0004674protein serine/threonine kinase activity ----
    GO:0004707MAP kinase activity ISS--
    GO:0004713protein tyrosine kinase activity ----
         
    NLK for ontologies           About GeneDecksing


    Phenotypes:
         6 GenomeRNAi human phenotypes for NLK:
     Decreased Hepatitis C virus re  Decreased TP53 protein express  Decreased Wnt reporter activit  Decreased focal adhesion (FA)  
     Decreased melanin production  Increased apoptosis 

         7 MGI mutant phenotypes (inferred from 1 allele(MGI details for Nlk):
     behavior/neurological  cellular  growth/size  hematopoietic system  immune system 
     mortality/aging  skeleton 

    NLK for phenotypes           About GeneDecksing

    Animal Models:
       inGenious Targeting Laboratory - Custom generated mouse model solutions for NLK 
       inGenious Targeting Laboratory - Custom generated inducible mouse model solutions for NLK

       genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for NLK 
       genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for NLK 

    miRNA
    Products:
        
    miRTarBase miRNAs that target NLK:
    hsa-mir-181b (MIRT000238), hsa-mir-181a (MIRT000243), hsa-mir-181c (MIRT000233)

    QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat NLK
    8/139 QIAGEN miScript miRNA Assays for microRNAs that regulate NLK (see all 139):
    hsa-miR-140-5p hsa-miR-199a-3p hsa-miR-605 hsa-miR-3921 hsa-miR-502-3p hsa-miR-208b hsa-miR-508-5p hsa-miR-92b
    SwitchGear 3'UTR luciferase reporter plasmidNLK 3' UTR sequence
    Inhib. RNA
    Products:
        
    Browse for Gene Knock-down Tools from EMD Millipore
    OriGene RNAi products in human, mouse, rat for NLK
    QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat NLK

    Gene Editing
    Products:
    DNA2.0 Custom Protein Engineering Service for NLK
    Sirion Biotech Customized adenovirus for overexpression of NLK

    Clone
    Products:
         
    Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore
    OriGene clones in human, mouse for NLK (see all 10)
    OriGene ORF clones in mouse, rat for NLK
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector: NLK (NM_016231)
    Sino Biological Human cDNA Clone for NLK
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for NLK
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat NLK
    Sirion Biotech Customized lentivirus for stable overexpression of NLK 
                         Customized lentivirus expression plasmids for stable overexpression of NLK 

    Cell Line
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    GenScript Custom overexpressing Cell Line Services for NLK
    Search LifeMap BioReagents cell lines for NLK
    In Situ Assay
    Products:
       

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for NLK


    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Oct 2013 via Entrez Gene).
    About This Section

    SuperPaths for NLK About   (see all 11)                                                                                              See pathways by source

    SuperPathContained pathways About
    1Wnt signaling pathway
    Wnt signaling pathway0.40
    Wnt Signaling Pathway NetPath0.37
    Wnt Signaling Pathway0.40
    2MAPK signaling pathway
    MAPK signaling pathway0.50
    MAPK signaling pathway0.50
    3Wnt Signaling Pathway
    Wnt Signaling Pathway and Pluripotency0.55
    4Canonical Wnt Pathway
    Non-Canonical Wnt Pathway0.53
    5Rho Family GTPases
    Molecular Mechanisms of Cancer0.51

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways

    2 R&D Systems Pathways for NLK
        Wnt Signaling Pathway
    Notch Signaling Pathways

    1 Downloadable PowerPoint Slide of QIAGEN Pathway Central Maps for NLK
        Molecular Mechanisms of Cancer

    5/7 BioSystems Pathways for NLK (see all 7)
        MAPK signaling pathway
    Wnt Signaling Pathway and Pluripotency
    Wnt Signaling Pathway NetPath
    IL-6 Signaling Pathway
    C-MYB transcription factor network

    1 Sino Biological Pathway for NLK 
        Non-Canonical Wnt Pathway


    3         Kegg Pathways  (Kegg details for NLK):
        MAPK signaling pathway
    Wnt signaling pathway
    Adherens junction


    NLK for pathways           About GeneDecksing

    Interactions:

        SABiosciences Gene Network CentralTM Interacting Genes and Proteins Network for NLK

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    5/53 Interacting proteins for NLK (Q9UBE81, 2, 3 ENSP000003846254) via UniProtKB, MINT, STRING, and/or I2D (see all 53)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    ATXN1P542532, 3, ENSP000002447694MINT-2873516 I2D: score=3 STRING: ENSP00000244769
    MYBP102423, ENSP000003399924I2D: score=4 STRING: ENSP00000339992
    ASGR1P073063, ENSP000002692994I2D: score=2 STRING: ENSP00000269299
    CUL1Q136163, ENSP000003268044I2D: score=2 STRING: ENSP00000326804
    SKP2Q133093, ENSP000002742554I2D: score=2 STRING: ENSP00000274255
    About this table

    Gene Ontology (GO): 5/11 biological process terms (GO ID links to tree view) (see all 11):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000165MAPK cascade ----
    GO:0006351transcription, DNA-dependent IEA--
    GO:0006355regulation of transcription, DNA-dependent ISS--
    GO:0006468protein phosphorylation ISS--
    GO:0007179transforming growth factor beta receptor signaling pathway IMP15004007

    NLK for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section

    NLK for compounds           About GeneDecksing

    Browse Small Molecules at EMD Millipore
    Browse drugs & compounds from Enzo Life Sciences

    Browse Tocris compounds for NLK

    1 HMDB Compound for NLK    About this table
    CompoundSynonyms CAS #PubMed Ids
    MagnesiumMagnesium (see all 2)7439-95-4--

    2 Novoseek inferred chemical compound relationships for NLK gene    About this table
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    threonine 54 8 12901858 (1), 14720327 (1), 15870890 (1), 12556497 (1) (see all 5)
    serine 41.3 8 12901858 (1), 14720327 (1), 15870890 (1), 12556497 (1) (see all 5)

    Search CenterWatch for drugs/clinical trials and news about NLK

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    Conferences by KenesGroup, exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Sirion Biotech, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section

    REFSEQ mRNAs for NLK gene: 
    NM_016231.4  

    Unigene Cluster for NLK:

    Nemo-like kinase
    Hs.208759  [show with all ESTs]
    Unigene Representative Sequence: BC064663
    6 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000583517 ENST00000407008(uc010crj.3) ENST00000582037 ENST00000496808
    ENST00000584188 ENST00000584878
    miRNA
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    QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat NLK
    8/139 QIAGEN miScript miRNA Assays for microRNAs that regulate NLK (see all 139):
    hsa-miR-140-5p hsa-miR-199a-3p hsa-miR-605 hsa-miR-3921 hsa-miR-502-3p hsa-miR-208b hsa-miR-508-5p hsa-miR-92b
    SwitchGear 3'UTR luciferase reporter plasmidNLK 3' UTR sequence
    Inhib. RNA
    Products:
         
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    Clone
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    GenScript: all cDNA clones in your preferred vector: NLK (NM_016231)
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for NLK
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat NLK
    Sirion Biotech Customized lentivirus for stable overexpression of NLK 
                         Customized lentivirus expression plasmids for stable overexpression of NLK 
    Primer
    Products:
        
    OriGene qPCR primer pairs and template standards for NLK
    OriGene qSTAR qPCR primer pairs in human, mouse for NLK
    SABiosciences RT2 qPCR Primer Assay in human, mouse, rat NLK
      QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat NLK
      QIAGEN QuantiFast Probe-based Assays in human, mouse, rat NLK

    Additional mRNA sequence: 

    AF180819.1 AF197898.1 AK024686.1 AK130232.1 AK315315.1 AL161970.1 BC064663.1 

    5 DOTS entries:

    DT.452078  DT.95175455  DT.86845958  DT.455470  DT.91902024 

    24/185 AceView cDNA sequences (see all 185):

    AW473308 AI634833 CA313532 N44198 CA313565 BQ186442 AA465119 AA425925 
    CA444839 AA909224 AI950254 AI057518 AW299913 AF197898 BF475834 AA586551 
    BE827564 AL711916 AA243380 AA744696 AA054626 AW003158 BE045818 AI418499 

    GeneLoc Exon Structure


    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    NLK expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: CTGATGATGA
    NLK Expression
    About this image


    See NLK Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for NLK

    SOURCE GeneReport for Unigene cluster: Hs.208759
        SABiosciences Expression via Pathway-Focused PCR Array including NLK: 
              WNT Signaling Pathway in human mouse rat

    Primer
    Products:
    OriGene qPCR primer pairs and template standards for NLK
    OriGene qSTAR qPCR primer pairs in human, mouse for NLK
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    QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat NLK
    QIAGEN QuantiFast Probe-based Assays in human, mouse, rat NLK
    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for NLK

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the common ancestor of eukaryotes.

    Orthologs for NLK gene from 9/22 species (see all 22)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Nlk1 , 5 nemo like kinase1, 5 94.43(n)1
    99.81(a)1
      11 (46.74 cM)5
    180991  NM_008702.31  NP_032728.31 
     785671685 
    chicken
    (Gallus gallus)
    Aves NLK1 nemo-like kinase 89.75(n)
    98.67(a)
      417669  XM_415915.3  XP_415915.3 
    lizard
    (Anolis carolinensis)
    Reptilia NLK6
    --
    Uncharacterized protein
    98(a)
    83(a)
    1 ↔ 1
    possible ortholog
    GL343729.1(241-102116)
    GL343606.1(144404-160817)
    African clawed frog
    (Xenopus laevis)
    Amphibia xnlk2 nemo-like kinase 74.19(n)    AB071285.1 
    zebrafish
    (Danio rerio)
    Actinopterygii nlk21 nemo like kinase, type 2 76.74(n)
    94.67(a)
      563460  XM_002664643.1  XP_002664689.1 
    fruit fly
    (Drosophila melanogaster)
    Insecta nmo1 , 3 ommatidial rotation protein
    serine/threonine kinase3
    nemo1
    78(a)3
    64.68(n)1
    77.57(a)1
      388901  NM_168250.21  NP_729318.11 
    worm
    (Caenorhabditis elegans)
    Secernentea lit-11 Protein LIT-1 58.35(n)
    58.82(a)
      176808  NM_001027634.1  NP_001022805.1 
    baker's yeast
    (Saccharomyces cerevisiae)
    Saccharomycetes FUS36
    KSS16
    Mitogen-activated serine/threonine protein kinase ...
    Mitogen-activated protein kinase (MAPK) involved i...
    39(a)
    38(a)
    many ↔ many
    many ↔ many
    II(192451-193512)
    VII(575398-576504)
    rice
    (Oryza sativa)
    Liliopsida AF216316.12   -- 75.94(n)    AF216316.1 


    ENSEMBL Gene Tree for NLK (if available)
    TreeFam Gene Tree for NLK (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    Paralogs for NLK gene
    MAPK42  MAPK32  MAPK72  MAPK152  MAPK62  MAPK12  
    18/47 SIMAP similar genes for NLK using alignment to 3 protein entries:     NLK_HUMAN (see all proteins) (see all similar genes):
    TXK    DKFZp686O0215    MAPK1    DYRK4    MAPK14    MAPK11
    MAPK3    MAPK8    MAPK13    NEK9    CDK7    STK11
    CDC2    MAPK7    MAPK9    MAPK10    MOK    RPS6KA2

    NLK for paralogs           About GeneDecksing



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/2416 SNPs in NLK are shown (see all 2416)    About this table     
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 17 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    VAR_0422734
    A glioblastoma multiforme sample4--see VAR_0422732 A T mis40--------
    rs341546511,2
    C,F,H--26367840(+) CATTTG/TTCGAC 1 -- us2k16Minor allele frequency- T:0.01NS NA 496
    rs356673371,2
    C,F--26368235(+) AGATCT/CAAAGG 1 -- us2k13Minor allele frequency- C:0.03NS NA 214
    rs790646411,2
    F--26368256(+) CATCAA/TCCTTT 1 -- us2k10--------
    rs346907631,2
    C,F--26368257(+) ATCAAC/TCTTTT 1 -- us2k15Minor allele frequency- T:0.14NS CSA WA 215
    rs1867578741,2
    --26368320(+) ATTTTC/TAACCC 1 -- us2k10--------
    rs1916219861,2
    --26368360(+) GGCTCA/TTGATT 1 -- us2k10--------
    rs346848051,2
    C,F--26368445(+) AAAAAA/TAAATA 1 -- us2k13Minor allele frequency- T:0.04NS WA 212
    rs1847470821,2
    --26368446(+) AAAAAA/TAATAA 1 -- us2k10--------
    rs340661241,2
    C,F--26368492(+) GAAAGA/-AAAAT 1 -- us2k12Minor allele frequency- -:0.04NS 94

    HapMap Linkage Disequilibrium report for NLK (26368763 - 26523407 bp)

    Structural Variations
         Database of Genomic Variants (DGV) 3 variations for NLK:    About this table     
    Variant IDTypeSubtypePubMed ID
    esv2678164CNV Deletion23128226
    nsv2016CNV Loss18451855
    nsv522349CNV Gain19592680

    SABiosciences Cancer Mutation PCR Assays
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    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Conferences by KenesGroup, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section
    OMIM gene information: 609476    OMIM disorders: --

    10 diseases for NLK:    About MalaCards
    neurofibromatosis    colon cancer    retinitis    gastric cancer
    colorectal cancer    ovarian cancer    prostate cancer    prostatitis
    hepatitis    neuronitis


    NLK for disorders           About GeneDecksing

    2 Novoseek inferred disease relationships for NLK gene    About this table

    Disease   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    colon cancer 27.7 6 12901858 (1), 14720327 (1), 20512928 (1)
    cancer 7.8 2 20512928 (1)

    Genetic Association Database (GAD): NLK
    Human Genome Epidemiology (HuGE) Navigator: NLK (7 documents)

    Export disorders for NLK gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for NLK gene, integrated from 9 sources (see all 66):
    (articles sorted by number of sources associating them with NLK)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Characterization of the Fugu rubripes NLK and FN5 genes flanking the NF1 (Neurofibromatosis type 1) gene in the 5' direction and mapping of the human counterparts. (PubMed id 10863097)1, 2, 3, 9 Kehrer-Sawatzki H....Krone W. (2000)
    2. STAT3 regulates Nemo-like kinase by mediating its interaction with IL-6-stimulated TGFbeta-activated kinase 1 for STAT3 Ser-727 phosphorylation. (PubMed id 15764709)1, 2, 9 Kojima H....Nakajima K. (2005)
    3. Wnt-1 signal induces phosphorylation and degradation of c-Myb protein via TAK1, HIPK2, and NLK. (PubMed id 15082531)1, 2, 9 Kanei-Ishii C.... Ishii S. (2004)
    4. Regulation of FOXO1 by TAK1-Nemo-like kinase pathway. (PubMed id 20061393)1, 2, 9 Kim S....Chung J. (2010)
    5. A histone lysine methyltransferase activated by non-canonical Wnt signalling suppresses PPAR-gamma transactivation. (PubMed id 17952062)1, 2, 9 Takada I....Kato S. (2007)
    6. Zipper-interacting protein kinase (ZIPK) modulates canonical Wnt/beta-catenin signaling through interaction with Nemo-like kinase and T-cell factor 4 (NLK/TCF4). (PubMed id 21454679)1, 2 Togi S.... Matsuda T. (2011)
    7. Oxidative stress-dependent regulation of Forkhead box O4 activity by nemo-like kinase. (PubMed id 20874444)1, 2 Szypowska A.A....Burgering B.M.T. (2011)
    8. Nemo-like kinase suppresses Notch signalling by inter fering with formation of the Notch active transcriptional complex. (PubMed id 20118921)1, 2 Ishitani T....Itoh M. (2010)
    9. Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. (PubMed id 20379614)1, 4 Rose J.E....Uhl G.R. (2010)
    10. Single nucleotide polymorphisms in the Wnt and BMP pa thways and colorectal cancer risk in a Spanish cohort. (PubMed id 20844743)1, 4 FernA!ndez-Rozadilla C....Ruiz-Ponte C. (2010)

    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section
     ANDOR
    Aliases
    Disorders
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 51701 HGNC: 29858 AceView: NLK Ensembl:ENSG00000087095 euGenes: HUgn51701
    ECgene: NLK Kegg: 51701 H-InvDB: NLK

    (According to HUGE)
    About This Section
      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for NLK Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section
    Patent Information for NLK gene:
    Search GeneIP for patents involving NLK

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



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    About This Section

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    GeneCards Homepage - Last full update: 23 Oct 2013 - Incrementals: 4 Nov 2013 , 7 Nov 2013 , 23 Jan 2014

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