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Aliases for NLE1 Gene

Aliases for NLE1 Gene

  • Notchless Homolog 1 2 3 5
  • Notchless Gene Homolog, (Drosophila) 2
  • Notchless Homolog 1 (Drosophila) 2
  • Notchless Protein Homolog 1 3
  • Notchless Gene Homolog 3
  • Nle 3

External Ids for NLE1 Gene

Previous GeneCards Identifiers for NLE1 Gene

  • GC17M030472
  • GC17M033458
  • GC17M029643

Summaries for NLE1 Gene

GeneCards Summary for NLE1 Gene

NLE1 (Notchless Homolog 1) is a Protein Coding gene. An important paralog of this gene is TEP1.

UniProtKB/Swiss-Prot for NLE1 Gene

  • Plays a role in regulating Notch activity. Plays a role in regulating the expression of CDKN1A and several members of the Wnt pathway, probably via its effects on Notch activity. Required during embryogenesis for inner mass cell survival (By similarity).

Gene Wiki entry for NLE1 Gene

No data available for Entrez Gene Summary , Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for NLE1 Gene

Genomics for NLE1 Gene

Regulatory Elements for NLE1 Gene

Enhancers for NLE1 Gene
GeneHancer Identifier Enhancer Score Enhancer Sources Gene-Enhancer Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor Binding Sites within enhancer Gene Targets for Enhancer
GH17F035212 1.2 FANTOM5 Ensembl ENCODE 21.6 -72.8 -72777 5.4 PKNOX1 ZNF133 ETV1 CEBPG GABPA OSR2 ZNF157 TRIM28 SPI1 SLFN5 NLE1 SLFN12L ENSG00000267321 UNC45B ZNF830 SLC35G3
GH17F035171 0.9 Ensembl ENCODE 24.1 -29.6 -29646 1.3 CTCF ZNF654 RNF2 CEBPB RAD21 ATF2 JUND ATF3 SMC3 NLE1 SLFN5 SLFN12L GC17M035166 GC17M035176
GH17F035020 1.3 FANTOM5 Ensembl ENCODE 10.2 +119.6 119556 3.9 BCOR CTCF JUN ZMYM3 SIN3A ZIC2 RAD21 POLR2A FOS MAFK LIG3 RNU6-840P SLFN12L NLE1 SLFN5 ZNF830 CCT6B RFFL ENSG00000273687 GC17P035052
GH17F035529 1.3 FANTOM5 Ensembl ENCODE 9.8 -388.8 -388839 3.6 CTCF ZNF146 REST ZBTB40 FOSL1 ZNF316 ZNF366 ZBTB33 ZNF24 PRDM1 SLFN5 AP2B1 SLFN13 NLE1 SLFN12L RASL10B SLFN12 CCL5 SLFN11 RFFL
- Elite enhancer and/or Elite enhancer-gene association Download GeneHancer data dump

Enhancers around NLE1 on UCSC Golden Path with GeneCards custom track

Promoters for NLE1 Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters
ENSR00001344726 614 2199 HDGF CREB3L1 MLX ARID4B SIN3A DMAP1 ZNF2 YY1 SLC30A9 ZNF207

Genomic Location for NLE1 Gene

35,128,753 bp from pter
35,142,315 bp from pter
13,563 bases
Minus strand

Genomic View for NLE1 Gene

Genes around NLE1 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
NLE1 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for NLE1 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for NLE1 Gene

Proteins for NLE1 Gene

  • Protein details for NLE1 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Recommended name:
    Notchless protein homolog 1
    Protein Accession:
    Secondary Accessions:
    • O60868
    • Q59GJ8
    • Q9BU54

    Protein attributes for NLE1 Gene

    485 amino acids
    Molecular mass:
    53320 Da
    Quaternary structure:
    No Data Available
    • Sequence=BAD92348.1; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305};

    Alternative splice isoforms for NLE1 Gene


neXtProt entry for NLE1 Gene

Post-translational modifications for NLE1 Gene

  • Ubiquitination at Lys 224 and Lys 326
  • Modification sites at PhosphoSitePlus

No data available for DME Specific Peptides for NLE1 Gene

Domains & Families for NLE1 Gene

Gene Families for NLE1 Gene

Suggested Antigen Peptide Sequences for NLE1 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry



  • Belongs to the NLE1/RSA4 family.
  • Contains 8 WD repeats.
  • Belongs to the NLE1/RSA4 family.
  • Contains 8 WD repeats.
genes like me logo Genes that share domains with NLE1: view

Function for NLE1 Gene

Molecular function for NLE1 Gene

UniProtKB/Swiss-Prot Function:
Plays a role in regulating Notch activity. Plays a role in regulating the expression of CDKN1A and several members of the Wnt pathway, probably via its effects on Notch activity. Required during embryogenesis for inner mass cell survival (By similarity).
genes like me logo Genes that share phenotypes with NLE1: view

Animal Models for NLE1 Gene

MGI Knock Outs for NLE1:

Animal Model Products

Inhibitory RNA Products

Flow Cytometry Products

No data available for Enzyme Numbers (IUBMB) , Gene Ontology (GO) - Molecular Function , Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for NLE1 Gene

Localization for NLE1 Gene

Subcellular locations from UniProtKB/Swiss-Prot for NLE1 Gene

Nucleus, nucleolus.

Subcellular locations from

Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for NLE1 gene
Compartment Confidence
nucleus 5
cytosol 3
plasma membrane 2
extracellular 2
lysosome 1

Gene Ontology (GO) - Cellular Components for NLE1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus IEA,IDA --
GO:0005730 nucleolus IEA,IDA --
GO:0005840 ribosome IBA --
genes like me logo Genes that share ontologies with NLE1: view

Pathways & Interactions for NLE1 Gene

No Data Available

Gene Ontology (GO) - Biological Process for NLE1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000027 ribosomal large subunit assembly IBA --
GO:0001756 somitogenesis IEA --
GO:0001822 kidney development IEA --
GO:0001826 inner cell mass cell differentiation IEA --
GO:0007219 Notch signaling pathway IEA,IBA --
genes like me logo Genes that share ontologies with NLE1: view

No data available for Pathways by source and SIGNOR curated interactions for NLE1 Gene

Transcripts for NLE1 Gene

Unigene Clusters for NLE1 Gene

Notchless homolog 1 (Drosophila):
Representative Sequences:

Inhibitory RNA Products

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for NLE1 Gene

ExUns: 1a · 1b · 1c ^ 2a · 2b ^ 3 ^ 4 ^ 5 ^ 6a · 6b · 6c ^ 7 ^ 8 ^ 9 ^ 10 ^ 11 ^ 12 ^ 13
SP1: - -
SP3: - -
SP5: - -

Relevant External Links for NLE1 Gene

GeneLoc Exon Structure for
ECgene alternative splicing isoforms for

Expression for NLE1 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for NLE1 Gene

mRNA differential expression in normal tissues according to GTEx for NLE1 Gene

This gene is overexpressed in Pancreas (x4.1).

Protein differential expression in normal tissues from HIPED for NLE1 Gene

This gene is overexpressed in Breast (21.0), Adipocyte (15.5), and Peripheral blood mononuclear cells (8.7).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MaxQB, and MOPED for NLE1 Gene

Protein tissue co-expression partners for NLE1 Gene

NURSA nuclear receptor signaling pathways regulating expression of NLE1 Gene:


SOURCE GeneReport for Unigene cluster for NLE1 Gene:

genes like me logo Genes that share expression patterns with NLE1: view

Primer Products

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery and mRNA Expression by UniProt/SwissProt for NLE1 Gene

Orthologs for NLE1 Gene

This gene was present in the common ancestor of eukaryotes.

Orthologs for NLE1 Gene

Organism Taxonomy Gene Similarity Type Details
(Pan troglodytes)
Mammalia NLE1 34 35
  • 99.52 (n)
(Bos Taurus)
Mammalia NLE1 34 35
  • 91.28 (n)
(Canis familiaris)
Mammalia NLE1 34 35
  • 91.13 (n)
(Rattus norvegicus)
Mammalia Nle1 34
  • 89.55 (n)
(Mus musculus)
Mammalia Nle1 34 16 35
  • 89.14 (n)
(Monodelphis domestica)
Mammalia NLE1 35
  • 84 (a)
(Gallus gallus)
Aves NLE1 34 35
  • 76.65 (n)
(Anolis carolinensis)
Reptilia NLE1 35
  • 74 (a)
tropical clawed frog
(Silurana tropicalis)
Amphibia nle1 34
  • 73.32 (n)
Str.11153 34
African clawed frog
(Xenopus laevis)
Amphibia nle-pending-prov 34
(Danio rerio)
Actinopterygii nle1 34 35
  • 73.32 (n)
wufc12b09 34
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP002221 34
  • 60.82 (n)
fruit fly
(Drosophila melanogaster)
Insecta Nle 34 35
  • 59.66 (n)
(Caenorhabditis elegans)
Secernentea W07E6.2 34 35
  • 58.21 (n)
A. gosspyii yeast
(Ashbya gossypii)
Saccharomycetes AGOS_AER263C 34
  • 56.15 (n)
K. lactis yeast
(Kluyveromyces lactis)
Saccharomycetes KLLA0C08547g 34
  • 52.62 (n)
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes RSA4 34 35
  • 50.73 (n)
thale cress
(Arabidopsis thaliana)
eudicotyledons AT5G52820 34
  • 54.43 (n)
(Oryza sativa)
Liliopsida Os10g0104500 34
  • 60.3 (n)
(Hordeum vulgare)
Liliopsida Hv.6600 34
(Triticum aestivum)
Liliopsida Ta.10544 34
sea squirt
(Ciona savignyi)
Ascidiacea -- 35
  • 66 (a)
bread mold
(Neurospora crassa)
Ascomycetes NCU00794 34
  • 52.4 (n)
fission yeast
(Schizosaccharomyces pombe)
Schizosaccharomycetes SPCC18.05c 34
  • 51.35 (n)
sea squirt
(Ciona intestinalis)
Ascidiacea Cin.13874 34
Species where no ortholog for NLE1 was found in the sources mined by GeneCards:
  • Actinobacteria (Mycobacterium tuberculosis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • beta proteobacteria (Neisseria meningitidis)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • platypus (Ornithorhynchus anatinus)
  • rainbow trout (Oncorhynchus mykiss)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)

Evolution for NLE1 Gene

Gene Tree for NLE1 (if available)
Gene Tree for NLE1 (if available)

Paralogs for NLE1 Gene

Paralogs for NLE1 Gene

(12) SIMAP similar genes for NLE1 Gene using alignment to 4 proteins:

genes like me logo Genes that share paralogs with NLE1: view

Variants for NLE1 Gene

Sequence variations from dbSNP and Humsavar for NLE1 Gene

SNP ID Clin Chr 17 pos Sequence Context AA Info Type
rs75635495 A breast cancer sample 35,136,371(+) TCCTT(G/T)GAGGT reference, missense
rs10153277 -- 35,137,235(+) GGCAA(A/C/T)ATCTG intron-variant
rs10445390 -- 35,144,166(+) tagct(C/T)tgaat upstream-variant-2KB
rs111531476 -- 35,131,449(+) AGGCA(C/G)GGTAC utr-variant-3-prime
rs112042456 -- 35,140,527(+) CCCAG(A/C/T)CTAGC intron-variant

Structural Variations from Database of Genomic Variants (DGV) for NLE1 Gene

Variant ID Type Subtype PubMed ID
esv3306040 CNV mobile element insertion 20981092
esv3410861 CNV insertion 20981092
esv3892998 CNV loss 25118596
nsv2036 CNV insertion 18451855

Variation tolerance for NLE1 Gene

Residual Variation Intolerance Score: 43.9% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 5.43; 71.34% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for NLE1 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for NLE1 Gene

Disorders for NLE1 Gene

Relevant External Links for NLE1

Atlas of Genetics and Cytogenetics in Oncology and Haematology:

No disorders were found for NLE1 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for NLE1 Gene

Publications for NLE1 Gene

  1. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PMID: 14702039) Ota T. … Sugano S. (Nat. Genet. 2004) 3 4 64
  2. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PMID: 15489334) Gerhard D.S. … Malek J. (Genome Res. 2004) 3 4 64
  3. Functional proteomic analysis of human nucleolus. (PMID: 12429849) Scherl A. … Diaz J.-J. (Mol. Biol. Cell 2002) 3 4 64
  4. Characterization of 16 novel human genes showing high similarity to yeast sequences. (PMID: 11124703) Stanchi F. … Valle G. (Yeast 2001) 3 4 64
  5. The Crystal Structure of the Ubiquitin-like Domain of Ribosome Assembly Factor Ytm1 and Characterization of Its Interaction with the AAA-ATPase Midasin. (PMID: 26601951) Romes E.M. … Stanley R.E. (J. Biol. Chem. 2016) 3 64

Products for NLE1 Gene

Sources for NLE1 Gene

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