NKAP Gene
protein-coding GIFtS : 52
GCID: GC0X M119059
NFKB activating protein
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Aliasesfor NKAP gene
(According to
1 HGNC ,
2 Entrez Gene ,
3 UniProtKB/Swiss-Prot ,
4 UniProtKB/TrEMBL , 5 OMIM , 6 GeneLoc ,
7 Ensembl ,
8 DME ,
9 miRBase ,
and/or 10 fRNAdb )About This Section Aliases NFKB Activating Protein 1 2 FLJ226261 NF-Kappa-B-Activating Protein2 NF-KappaB Activating Protein2
Export aliases for NKAP gene to outside databases Previous GC identifers: GC0XM117813 GC0XM118840 GC0XM118943 GC0XM108555
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Summariesfor NKAP gene (According to Entrez Gene ,
Tocris Bioscience ,
Wikipedia's
Gene Wiki ,
PharmGKB ,
UniProtKB/Swiss-Prot ,
and/or
UniProtKB/TrEMBL )
About This Section Entrez Gene summary for NKAP : This gene encodes a protein that is involved in the activation of the ubiquitous transcription factor NF-kappaB. This protein is associated with the the histone deacetylase HDAC3 and with the Notch corepressor complex, and it thereby acts as a transcriptional repressor of Notch target genes. It is also required for alphabeta T cell development. A related pseudogene has been identified on chromosome X, while a related and intronless retrocopy, which has an intact CDS and may be functional, is located on chromosome 6. (provided by RefSeq, May 2010) UniProtKB/Swiss-Prot: NKAP_HUMAN, Q8N5F7 Function : Acts as a transcriptional repressor. Plays a role as a transcriptional corepressor of the Notch-mediatedsignaling required for T-cell development. Also involved in the TNF and IL-1 induced NF-kappa-B activation. Associates with chromatin at the Notch-regulated SKP2 promoter Gene Wiki entry for NKAP
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Genomic Viewsfor NKAP gene
(According to
GeneLoc and/or
HGNC , and/or
Entrez Gene (NCBI build 37) ,
and/or miRBase ,
Genomic Views according to
UCSC (hg19) and
Ensembl (release 69) ,
Regulatory elements and Epigenetics data according to
QIAGEN ,
SABiosciences , and/or
SwitchGear Genomics )About This Section RefSeq DNA sequence: NC_000023.10 NC_018934.1 NT_011786.16 Regulatory elements: SABiosciences Regulatory transcription factor binding sites in the NKAP gene promoter: C/EBPbeta p53 AP-1 ATF-2 GATA-1 MEF-2A SRY NRF-2 c-Jun aMEF-2 Other transcription factors Search SABiosciences Chromatin IP Primers for NKAP Epigenetics: QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human , mouse , rat NKAP
Genomic Location: Genomic View : UCSC Golden Path with GeneCards custom track Entrez Gene cytogenetic band: Xq24 Ensembl cytogenetic band: Xq24 HGNC cytogenetic band: Xq24 NKAP Gene in genomic location: bands according to Ensembl, locations according to
(and/or Entrez Gene and/or Ensembl if different) GeneLoc information about chromosome X GeneLoc Exon Structure
GeneLoc location for GC0XM119059: view genomic region
(about GC identifiers )
Start:
119,059,013 bp from pter
End:
119,077,735 bp from pter
Size:
18,723 bases
Orientation:
minus strand
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Proteinsfor NKAP gene
(According to
1 UniProtKB ,
HORDE ,
neXtProt ,
Ensembl ,
and/or Reactome ,
Modification sites according to 2 PhosphoSitePlus ,
Specific Peptides from DME ,
Protein expression images according to data from SPIRE MOPED and PaxDb ,
RefSeq according to NCBI ,
PDB rendering according to OCA and/or Proteopedia ,
Recombinant Proteins
from
EMD Millipore ,
R&D Systems ,
GenScript ,
Enzo Life Sciences ,
OriGene ,
Novus Biologicals ,
Sino Biological ,
ProSpec , and/or
Uscn ,
Biochemical Assays by
EMD Millipore ,
R&D Systems ,
OriGene ,
GenScript ,
Cell Signaling Technology ,
Enzo Life Sciences , and/or
Uscn ,
Ontologies according to Gene
Ontology Consortium 01 Mar 2013 and
Entrez Gene ,
Antibodies by
EMD Millipore ,
R&D Systems ,
GenScript ,
Cell Signaling Technology ,
OriGene ,
Novus Biologicals ,
Thermo Fisher Scientific ,
Abcam , and/or
Uscn )
About This Section UniProtKB/Swiss-Prot: NKAP_HUMAN, Q8N5F7 (See
protein sequence )Recommended Name: NF-kappa-B-activating protein Size : 415 amino acids; 47138 Da
Subunit : Component of the Notch corepressor complex. Interacts with CIR1 and HDAC3
Subcellular location : Nucleus
Sequence caution : Sequence=AAH71686.1; Type=Miscellaneous discrepancy; Note=Contaminating sequence. Potential poly-Asequence;
Secondary accessions : Q6IPW6 Q96BQ2 Q9H638Explore the universe of human proteins at neXtProt for NKAP: NX_Q8N5F7 Post-translational modifications:
View modification sites using PhosphoSitePlus 2 View neXtProt modification sites for NX_Q8N5F7 NKAP Protein expression data from MOPED and PaxDb : About this image
REFSEQ proteins: NP_078804.2 ENSEMBL proteins: ENSP00000360464 Human Recombinant Protein Products: Gene Ontology (GO): 3 cellular component terms (GO ID links to tree view) : About this table
NKAP for ontologies About GeneDecksing NKAP Antibody Products: Assay Products for NKAP:
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Protein
Domains / Familiesfor NKAP gene (According to InterPro , ProtoNet ,
UniProtKB , and/or BLOCKS ,
Sets of similar genes according to GeneDecks )
About This Section
NKAP for domains About GeneDecksing 1 InterPro domain/family :
Graphical View of Domain Structure for InterPro Entry Q8N5F7 ProtoNet protein and cluster: Q8N5F7
UniProtKB/Swiss-Prot: NKAP_HUMAN, Q8N5F7 Similarity : Belongs to the UPF0396 family
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Functionfor NKAP gene
(According to 1 UniProtKB ,
Genatlas ,
LifeMap Discovery™ ,
IUBMB , and/or
2 DME ,
Human phenotypes from GenomeRNAi ,
Animal models from MGI Mar 06 2013,
bound targets from SABiosciences ,
miRNA Gene Targets from miRTarBase
shRNA from
OriGene ,
RNAi from
EMD Millipore ,
siRNAs from
OriGene ,
QIAGEN ,
microRNA from QIAGEN ,
Gene Editing from DNA2.0 ,
Clones from EMD Millipore ,
OriGene ,
SwitchGear Genomics ,
GenScript ,
Sino Biological ,
DNA2.0 ,
and Vector BioLabs ,
Cell Lines from GenScript ,
LifeMap BioReagents ,
In Situ Hybridization Assays from Advanced Cell Diagnostics ,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene .)
About This Section Function Summary: UniProtKB/Swiss-Prot: NKAP_HUMAN, Q8N5F7 Function : Acts as a transcriptional repressor. Plays a role as a transcriptional corepressor of the Notch-mediatedsignaling required for T-cell development. Also involved in the TNF and IL-1 induced NF-kappa-B activation. Associates with chromatin at the Notch-regulated SKP2 promoter
Clone Products: Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human , mouse , rat for NKAP (see all 3 ) OriGene untagged cDNA clones in CMV expression vector in human , mouse , rat for NKAPOriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling GenScript: all cDNA clones in your preferred vector: NKAP (NM_024528 ) Browse Sino Biological Human cDNA Clones DNA2.0 Custom Codon Optimized Gene
Synthesis Service for NKAP Vector BioLabs ready-to-use adenovirus/AAV for human , mouse , rat NKAP
In Situ Assay Products: Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for NKAP
Gene Ontology (GO): 3 molecular function terms (GO ID links to tree view) : About this table
NKAP for ontologies About GeneDecksing 6 GenomeRNAi human phenotypes for NKAP :Animal Models: 3 MGI mutant phenotypes (inferred from 1 allele ) (MGI details for Nkap) :
NKAP for phenotypes About GeneDecksing
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Pathways & Interactionsfor NKAP gene
(Pathways according to
EMD Millipore ,
R&D Systems ,
Cell Signaling Technology ,
KEGG ,
PharmGKB ,
BioSystems ,
Reactome ,
Tocris Bioscience ,
GeneGo (Thomson Reuters) ,
QIAGEN ,
and/or UniProtKB ,
Sets of similar genes according to GeneDecks ,
Interaction Networks according to
SABiosciences ,
and/or STRING ,
Interactions according to 1 UniProtKB ,
2 MINT ,
3 I2D , and/or
4 STRING ,
with links to IntAct and
Ensembl ,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene) .
About This Section Interactions: Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for NKAP STRING Interaction
Network Preview (showing 5 interactants - click image to see 22)5/27 Interacting proteins for NKAP (Q8N5F7 2 , 3 ENSP00000360464 4 ) via UniProtKB, MINT, STRING , and/or I2D (see all 27 )About this table Gene Ontology (GO): 5/10 biological process terms (GO ID links to tree view) (see all 10 ): About this table
GO ID Qualified GO term Evidence PubMed IDs GO:0000122 negative regulation of transcription from RNA polymerase II promoter
IEA -- GO:0006351 transcription, DNA-dependent
IEA -- GO:0007219 Notch signaling pathway
IEA -- GO:0019827 stem cell maintenance
IEA -- GO:0030097 hemopoiesis
-- --
NKAP for ontologies About GeneDecksing
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Drugs & Compoundsfor NKAP gene (Chemical Compounds according to UniProtKB , Enzo Life Sciences ,
EMD Millipore , Tocris Bioscience
HMDB ,
BitterDB , and/or
Novoseek , and Drugs according to
DrugBank ,
Enzo Life Sciences , and/or
PharmGKB , with drugs/clinical trials/news
search links to CenterWatch )
About This Section Browse Tocris compounds for NKAP Search CenterWatch for drugs/clinical trials and news about NKAP
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Transcriptsfor NKAP gene (Secondary structures according to
fRNAdb ,
GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 235 Homo sapiens; Mar 10 2013) or GenBank , RefSeq according to Entrez Gene ,
DOTS (version 10), and/or
AceView ,
transcript ids from Ensembl
with links to UCSC ,
exon structure from GeneLoc ,
alternative splicing isoforms according to ASD and/or
ECgene ,
RNAi Products from
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siRNAs from
OriGene ,
QIAGEN ,
shRNA from
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microRNA from QIAGEN ,
Tagged/untagged cDNA clones from
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SwitchGear Genomics ,
GenScript ,
DNA2.0 ,
Vector BioLabs ,
Primers from
OriGene ,
SABiosciences , and/or
QIAGEN
)About This Section REFSEQ mRNAs for NKAP gene: NM_024528.3 Unigene Cluster for NKAP:
NFKB activating protein Hs.522771 [show with all ESTs ] Unigene Representative Sequence: AY388958 3 Ensembl transcripts including schematic representations, and UCSC links where relevant : ENST00000477789 (uc004esg.3 ) ENST00000371410 (uc004esh.3 ) ENST00000482407 Clone Products: OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human , mouse , rat for NKAP (see all 3 ) OriGene untagged cDNA clones in CMV expression vector in human , mouse , rat for NKAPOriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling GenScript: all cDNA clones in your preferred vector: NKAP (NM_024528 ) DNA2.0 Custom Codon Optimized Gene
Synthesis Service for NKAP Vector BioLabs ready-to-use adenovirus/AAV for human , mouse , rat NKAP
Additional cDNA sequence: AK026279.1 AL832118.1 AY388958.1 BC000940.2 BC012770.2 BC015354.1 BC016783.1 BC018968.1 BC032442.1 BC071686.1
8 DOTS entries : DT.442725 DT.100021929
DT.302983 DT.91642770 DT.95157264 DT.95157265 DT.102828750 DT.75187903 24/138 AceView cDNA sequences (see all 138 ):
CD514746 BC000940 AL832118 NM_024528 BC012770 AI264835 AW612579 BU727215 CA419472 BX374887 CN484033 BQ651280 AK094446 CR620788 AL711409 BM915475 BF059113 AY388958 BU931241 CN483805 AA778785 AI362090 BM719648 BC016783 GeneLoc Exon Structure
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Expression for NKAP gene
(RNA expression data according to
H-InvDB ,
NONCODE ,
miRBase , and
RNAdb ,
Expression images according to data from
BioGPS ,
Illumina Human BodyMap , and
CGAP
SAGE ,
Sets of similar genes according to GeneDecks ,
in vivo and in vitro expression data from LifeMap Discovery™ ,
plus additional links to
Genevestigator , and/or
SOURCE , and/or
BioGPS , and/or
UniProtKB ,
PCR Arrays from
SABiosciences ,
Primers from
OriGene ,
SABiosciences , and/or
QIAGEN ,
In Situ Hybridization Assays from Advanced Cell Diagnostics )
About This Section NKAP expression in normal human tissues (normalized intensities) See probesets specificity/sensitivity at GeneAnnot About this image BioGPS CGAP TAG: GCTCCGGTGT
About this image NKAP expression in embryonic tissues and stem cells Expression by the Database of Embryonic development, Stem cell research, and
Regenerative medicine About this table 1 LifeMap In Vivo Development Anatomical Compartment/Cell Tissue Anatomical Compartment
Cell Category (developmental path) Heart Endocardium Endocardial Tube Cells Endocardium Expression: Positive Negative
Selective markerExperimental details:
Curated
Microarrays
In-situ hybridization
See NKAP Protein Expression from SPIRE MOPED and PaxDB Genevestigator expression for NKAP SOURCE GeneReport for Unigene cluster: Hs.522771 SABiosciences Custom PCR Arrays for NKAP Primer Products: OriGene genome-wide validated SYBR primer pairs in human , mouse , rat for NKAPBrowse OriGene validated miRNA SYBR primer pairs SABiosciences RT2 qPCR Primer Assay in human , mouse , rat NKAP QIAGEN QuantiTect SYBR Green Assays in human , mouse , rat NKAP QIAGEN QuantiFast Probe-based Assays in human , mouse , rat NKAP In Situ Assay Products: Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for NKAP
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Orthologsfor NKAP gene
(Orthologs according to
1,2 HomoloGene (2 older version, for species not in 1 newer version),
3 euGenes ,
4 SGD
,
5 MGI Mar 06 2013,
with possible further links to
Flybase
and/or
WormBase ,
and/or
6 Ensembl pan taxonomic compara ,
Gene Trees according to Ensembl and
TreeFam )
About This Section
This gene was present in the common ancestor of eukaryotes.
Orthologs for NKAP gene from 9/29 species (see all 29 ) About this table
ENSEMBL Gene Tree for NKAP (if available)TreeFam Gene Tree for NKAP (if available)
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Paralogsfor NKAP gene (Paralogs according to
1 HomoloGene , 2 Ensembl , and 3 SIMAP , Pseudogenes according to 4 Pseudogene.org Build 68)About This Section Paralogs for NKAP gene NKAPL 2 1 SIMAP similar gene for NKAP using alignment to 3 protein entries: NKAP_HUMAN (see all proteins ):NKAPL
NKAP for paralogs About GeneDecksing
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Genomic Variantsfor NKAP gene (SNPs/Variants according to the
1 NCBI SNP Database ,
2 Ensembl ,
3 PupaSUITE ,
UniProtKB , and
DNA2.0 ,
Linkage Disequilibrium by HapMap ,
Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants , Mutations from the Human Gene
Mutation Database (HGMD) and the Locus Specific Mutation
Databases (LSDB) , Blood group antigen gene mutations by BGMUT ,
Resequencing Primers from QIAGEN ,
Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences )
About This Section
Genomic Data Transcription Related Data Allele Frequencies SNP ID Valid Clinical significance Chr X pos Sequence # AA Chg Type More # Allele freq Pop Total sample More
HapMap Linkage Disequilibrium report for NKAP (119059013 - 119077735 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions) Database of Genomic Variants (DGV): 8 variations for NKAP 8 CNVs : 36996 96931 68023 83711 6746 96930 4167 68022 Locus Specific Mutation Databases (LSDB): NKAP
SABiosciences Cancer Mutation PCR Assays
QIAGEN SeqTarget long-range PCR primers in human , mouse , rat for resequencing NKAP
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Disorders
/ Diseasesfor NKAP gene
(in which this Gene is Involved, According to MalaCards ,
OMIM, UniProtKB ,
the University of Copenhagen DISEASES
database ,
Genatlas , GeneTests ,
GAD ,
HuGE Navigator ,
and/or TGDB .)
About This Section
NKAP for disorders About GeneDecksing
OMIM gene information: 300766
OMIM disorders : --2 diseases for NKAP : About MalaCards crohn's disease malaria Human Genome Epidemiology (HuGE) Navigator: NKAP (1 document)Export disorders for NKAP gene to outside databases
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Publicationsfor NKAP gene (in
PubMed .
Associations of this gene to articles via
1 Entrez Gene ,
2 UniProtKB/Swiss-Prot ,
3 HGNC ,
4 GAD ,
5 PharmGKB ,
6 HMDB ,
7 DrugBank ,
8 UniProtKB/TrEMBL ,
9 Novoseek , and/or
10 fRNAdb )
About This Section PubMed articles for NKAP gene, integrated from 9 sources (see all 26 ): (articles sorted by number of sources associating them with NKAP) Utopia : connect your pdf to the dynamic world of online information
Identification of a nuclear protein that promotes NF-kappaB activation. (PubMed id 14550261) 1 , 2 , 3 Chen D.... Shu H.-B. (2003) NKAP is a transcriptional repressor of notch signalin g and is required for T cell development. (PubMed id 19409814) 1 , 2 Pajerowski A.G....Shapiro V.S. (2009) Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. (PubMed id 17081983) 1 , 2 Olsen J.V....Mann M. (2006) The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334) 1 , 2 Gerhard D.S....Malek J. (2004) Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039) 1 , 2 Ota T.... Sugano S. (2004) NKAP is required for T cell maturation and acquisitio n of functional competency. (PubMed id 21624937) 1 Hsu F.C....Shapiro V.S. (2011) System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation. (PubMed id 21406692) 2 Rigbolt K.T....Blagoev B. (2011) Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis. (PubMed id 20068231) 2 Olsen J.V....Mann M. (2010) Systematic analysis of human protein complexes identi fies chromosome segregation proteins. (PubMed id 20360068) 1 Hutchins J.R....Peters J.M. (2010) Dynamics of cullin-RING ubiquitin ligase network revea led by systematic quantitative proteomics. (PubMed id 21145461) 1 Bennett E.J....Harper J.W. (2010)
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External Searches for NKAP gene
(in PubMed ,
OMIM , and NCBI Bookshelf ) About This Section
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Genome Databases showing NKAP gene
(According to
Entrez Gene ,
HGNC ,
AceView ,
euGenes ,
Ensembl ,
miRBase ,
ECgene ,
Kegg ,
and/or
H-InvDB )
About This Section
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Other Databases showing NKAP gene
(According to HUGE )
About This Section --
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Specialized Databases showing NKAP gene (According to PharmGKB ,
ATLAS , HORDE , IMGT , LEIDEN , UniProtKB/Swiss-Prot , and/or UniProtKB/TrEMBL ,Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot )About This Section
Name Description
PharmGKB entry for NKAP Pharmacogenomics, SNPs, Pathways
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About This Section Patent Information for NKAP gene: Search GeneIP for patents involving NKAP GeneCards and IP: Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search
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Productsfor NKAP gene (Antibodies, recombinant proteins, and assays from EMD Millipore , R&D Systems , OriGene , QIAGEN , GenScript , Cell Signaling Technology , SABiosciences , Novus Biologicals , Sino Biological , Enzo Life Sciences , Abcam , ProSpec , Uscn , Thermo Fisher Scientific , Gene Editing from DNA2.0 , Clones from EMD Millipore , OriGene , GenScript , Sino Biological , DNA2.0 , SwitchGear Genomics , Vector BioLabs , Cell lines from GenScript and LifeMap BioReagents , PCR Arrays from SABiosciences , Drugs and/or compounds from EMD Millipore , Tocris Bioscience , and/or
Enzo Life Sciences ), In Situ Hybridization Assays from Advanced Cell Diagnostics About This Section
Browse OriGene Antibodies OriGene shRNA RFP for NKAP OriGene 29mer shRNA kits in GFP-retroviral vector in human , mouse , rat for NKAP OriGene genome-wide validated SYBR primer pairs in human , mouse , rat for NKAP OriGene Protein Over-expression Lysate for NKAP Browse OriGene Fluorogenic Cell Assay Kits OriGene siRNA for NKAP OriGene 3'-UTR Clone for NKAP OriGene untagged cDNA clones in CMV expression vector in human , mouse , rat for NKAP OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human , mouse , rat for NKAP Browse OriGene GFP tagged cDNA clones in CMV expression vector Browse OriGene MicroRNA Expression Plasmids Browse OriGene basic RS shRNAs Browse OriGene validated miRNA SYBR primer pairs Browse OriGene full length recombinant human proteins expressed in human HEK293 cells OriGene custom cloning services - gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling OriGene Custom Antibody Services for NKAP OriGene Custom Protein Services for NKAP OriGene Custom Immunoassay Development
QIAGEN Custom miScript Target Protector blocks miRNA-binding site of in human, mouse, rat NKAP QIAGEN SeqTarget long-range PCR primers in human , mouse , rat for resequencing NKAP QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human , mouse , rat NKAP QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human , mouse , rat NKAP QIAGEN QuantiFast Probe-based Assays in human , mouse , rat NKAP QIAGEN QuantiTect SYBR Green Assays in human , mouse , rat NKAP
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NKAP Proteins, Antibodies, CLIAs, and ELISAs
Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for NKAP
ThermoFisher Antibodies for NKAP
Vector BioLabs ready-to-use adenovirus/AAV for human , mouse , rat NKAP
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Aliases for NKAP
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Expression for NKAP
Function for NKAP
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