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NKAIN4 Gene

protein-coding   GIFtS: 43
GCID: GC20M061869

Na+/K+ Transporting ATPase Interacting 4

(Previous name: chromosome 20 open reading frame 58)
(Previous symbol: C20orf58)
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(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
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Aliases
Na+/K+ Transporting ATPase Interacting 41 2     Chromosome 20 Open Reading Frame 581
C20orf581 2 3     bA261N11.22
FAM77A2 3     Sodium/Potassium-Transporting ATPase Subunit Beta-1-Interacting Protein 42
Na(+)/K(+)-Transporting ATPase Subunit Beta-1-Interacting Protein 42 3     Protein FAM77A3

External Ids:    HGNC: 161911   Entrez Gene: 1284142   Ensembl: ENSG000001011987   OMIM: 6128735   UniProtKB: Q8IVV83   

Export aliases for NKAIN4 gene to outside databases

Previous GC identifers: GC20M061337 GC20M058597


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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Entrez Gene summary for NKAIN4 Gene:
NKAIN4 is a member of a family of mammalian proteins (see NKAIN1; MIM 612871) with similarity to Drosophila Nkain
and interacts with the beta subunit of Na,K-ATPase (ATP1B1; MIM 182330) (Gorokhova et al., 2007 (PubMed
17606467)).(supplied by OMIM, Jun 2009)

GeneCards Summary for NKAIN4 Gene:
NKAIN4 (Na+/K+ transporting ATPase interacting 4) is a protein-coding gene. An important paralog of this gene is NKAIN1.




(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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RefSeq DNA sequence at NCBI GenBank:
NC_000020.10  NT_011362.11  NC_018931.2  
Regulatory elements:
   Regulatory transcription factor binding sites in the NKAIN4 gene promoter:
         AP-1   ATF-2   c-Jun   HNF-4alpha2   HNF-4alpha1   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidNKAIN4 promoter sequence
   Search Chromatin IP Primers for NKAIN4

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat NKAIN4


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 20q13.33   Ensembl cytogenetic band:  20q13.33   HGNC cytogenetic band: 20q13.33

NKAIN4 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
NKAIN4 gene location

GeneLoc information about chromosome 20         GeneLoc Exon Structure

GeneLoc location for GC20M061869:  view genomic region     (about GC identifiers)

Start:
61,872,136 bp from pter      End:
61,904,046 bp from pter
Size:
31,911 bases      Orientation:
minus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, Cloud-Clone Corp., eBioscience, and/or antibodies-online,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, Cloud-Clone Corp., eBioscience, and/or antibodies-online, Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, Cloud-Clone Corp, antibodies-online, and/or others.)
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UniProtKB/Swiss-Prot: NKAI4_HUMAN, Q8IVV8 (See protein sequence)
Recommended Name: Sodium/potassium-transporting ATPase subunit beta-1-interacting protein 4  
Size: 208 amino acids; 23240 Da
Subunit: Interacts with ATP1B1 (By similarity)
Secondary accessions: Q4VXQ6 Q9BQU8 Q9BQU9

Explore the universe of human proteins at neXtProt for NKAIN4: NX_Q8IVV8

REFSEQ proteins: NP_690603.3  
ENSEMBL proteins: 
 ENSP00000359336   ENSP00000359340   ENSP00000359330   ENSP00000359341  

NKAIN4 Human Recombinant Protein Products:

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antibodies-online peptides for NKAIN4

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antibodies-online antibodies for NKAIN4 (13 products) 

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antibodies-online kits for NKAIN4 (4 products) 


(According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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HGNC Gene Families:
NKAIN: Na+/K+ transporting ATPase interacting

1 InterPro protein domain:
 IPR008516 Na/K-Atpase_Interacting

Graphical View of Domain Structure for InterPro Entry Q8IVV8

ProtoNet protein and cluster: Q8IVV8

UniProtKB/Swiss-Prot: NKAI4_HUMAN, Q8IVV8
Similarity: Belongs to the NKAIN family


Find genes that share domains with NKAIN4           About GenesLikeMe


(According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, Vector BioLabs and/or Addgene, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Flow cytometry from eBioscience, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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Phenotypes:
     2 GenomeRNAi human phenotypes for NKAIN4:
 Decreased viability with pacli  Increased gamma-H2AX phosphory 

     1 MGI mutant phenotype (inferred from 1 allele(MGI details for Nkain4):
 no phenotypic analysis 

Find genes that share phenotypes with NKAIN4           About GenesLikeMe

Animal Models:
   inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for NKAIN4
   inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for NKAIN4

   genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for NKAIN4
   genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for NKAIN4

miRNA
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1 qRT-PCR Assays for microRNA that regulate NKAIN4:
hsa-miR-485-5p
SwitchGear 3'UTR luciferase reporter plasmidNKAIN4 3' UTR sequence
Inhib. RNA
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Predesigned siRNA for gene silencing in human, mouse, rat NKAIN4

Gene Editing
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Clone
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OriGene clones in human, mouse for NKAIN4 (see all 6)
OriGene ORF clones in mouse, rat for NKAIN4
OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
GenScript: all cDNA clones in your preferred vector: NKAIN4 (NM_152864)
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DNA2.0 Custom Codon Optimized Gene Synthesis Service for NKAIN4
Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat NKAIN4

Cell Line
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In Situ Assay
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Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for NKAIN4


(According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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Subcellular locations from UniProtKB/Swiss-Prot
NKAI4_HUMAN, Q8IVV8: Cell membrane; Multi-pass membrane protein (Potential)
Subcellular locations from COMPARTMENTS: 

CompartmentConfidence
plasma membrane5
extracellular1

Gene Ontology (GO): 2 cellular component terms:    About this table

GO IDQualified GO termEvidencePubMed IDs
GO:0005886plasma membrane IEA--
GO:0016021integral component of membrane IEA--

Find genes that share ontologies with NKAIN4           About GenesLikeMe


(SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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    Custom Pathway & Disease-focused RT2 Profiler PCR Arrays for NKAIN4
Interactions:

    Search GeneGlobe Interaction Network for NKAIN4

(Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
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Browse Small Molecules at EMD Millipore
   Browse drugs & compounds from Enzo Life Sciences
  Browse compounds at ApexBio 

Browse Tocris compounds for NKAIN4 (NKAI4)



(Secondary structures according to fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, and/or Addgene, Primers from OriGene, and/or QIAGEN, Flow cytometry from eBioscience )
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REFSEQ mRNAs for NKAIN4 gene: 
NM_152864.3  

Unigene Cluster for NKAIN4:

Na+/K+ transporting ATPase interacting 4
Hs.46627  [show with all ESTs]
Unigene Representative Sequence: AK308958
9 Ensembl transcripts including schematic representations, and UCSC links where relevant:
ENST00000370313 ENST00000370316(uc002yek.3) ENST00000466885 ENST00000470246
ENST00000370307 ENST00000370317 ENST00000486495(uc010gkd.1) ENST00000461738
ENST00000472670
miRNA
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1 qRT-PCR Assays for microRNA that regulate NKAIN4:
hsa-miR-485-5p
SwitchGear 3'UTR luciferase reporter plasmidNKAIN4 3' UTR sequence
Inhib. RNA
Products:
     
OriGene RNAi products in human, mouse, rat for NKAIN4
Predesigned siRNA for gene silencing in human, mouse, rat NKAIN4
Clone
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OriGene ORF clones in mouse, rat for NKAIN4
OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
GenScript: all cDNA clones in your preferred vector: NKAIN4 (NM_152864)
DNA2.0 Custom Codon Optimized Gene Synthesis Service for NKAIN4
Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat NKAIN4
Primer
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OriGene qSTAR qPCR primer pairs in human, mouse for NKAIN4
Pre-validated RT2 qPCR Primer Assay in human, mouse, rat NKAIN4
  QuantiTect SYBR Green Assays in human, mouse, rat NKAIN4
  QuantiFast Probe-based Assays in human, mouse, rat NKAIN4

Additional mRNA sequence: 

AK126246.1 AK225765.1 AK308958.1 BC041812.1 

10 DOTS entries:

DT.313230  DT.120811261  DT.120811254  DT.99952804  DT.120811258  DT.120811275  DT.120811259  DT.313228 
DT.92420627  DT.95080701 

Selected AceView cDNA sequences (see all 106):

AA424030 AI918153 AW591198 BE504796 BE857508 AI968164 BP350009 BE504963 
BF592130 BM696562 F04093 BE671805 AI433327 AI953726 BF739879 AI422871 
AI522193 BP350285 AI493014 BM692343 AI569665 BP349408 AI266443 AI651234 

GeneLoc Exon Structure

Selected Alternative Splicing Database (ASD) splice patterns (SP) for NKAIN4 (see all 11)    About this scheme

ExUns: 1 ^ 2a · 2b ^ 3a · 3b · 3c ^ 4a · 4b ^ 5a · 5b · 5c ^ 6 ^ 7 ^ 8a · 8b · 8c ^ 9 ^ 10a · 10b ^ 11 ^ 12 ^ 13a · 13b
SP1:                    -     -     -                 -                 -     -     -                 -     -     -                           
SP2:                    -     -     -                 -                 -     -     -                 -     -     -           -               
SP3:                                                  -                 -     -     -                 -                                       
SP4:                                                  -                 -     -     -                 -     -     -                           
SP5:                                                                    -     -     -                 -     -                                 


ECgene alternative splicing isoforms for NKAIN4

(RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
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NKAIN4 expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS <intensity>2/3
CGAP TAG: --
NKAIN4 Expression
About this image


NKAIN4 expression in embryonic tissues and stem cells    About this table
Data from LifeMap, the Embryonic Development and Stem Cells Database
 selected tissues (see all 5) fully expand
 
 Brain (Nervous System)    fully expand to see all 5 entries
         Thalamus
 
 Neural Tube (Nervous System)    fully expand to see all 4 entries
         Metencephalon
 
 Kidney (Urinary System)
         Metanephros
 
 Adipose (Muscoskeletal System)
         Visceral White Adipose
 
 Eye (Sensory Organs)
         Retina
NKAIN4 Protein expression data from MOPED1, PaxDb2 and MaxQB3 --

SOURCE GeneReport for Unigene cluster: Hs.46627
    Custom PCR Arrays for NKAIN4
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Products:
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In Situ
Assay Products:
 

 
Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for NKAIN4

(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
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This gene was present in the common ancestor of animals.

Orthologs for NKAIN4 gene from Selected species (see all 13)    About this table
Organism Taxonomic
classification
Gene Description Human
Similarity
Orthology
Type
Details
mouse
(Mus musculus)
Mammalia Nkain41 , 5 Na+/K+ transporting ATPase interacting 41, 5 82.53(n)1
83.17(a)1
  2 (103.53 cM)5
582371  NM_021426.41  NP_067401.31 
 1809347725 
chicken
(Gallus gallus)
Aves NKAIN41 Na+/K+ transporting ATPase interacting 4 74.36(n)
72.6(a)
  419240  XM_003642483.2  XP_003642531.1 
lizard
(Anolis carolinensis)
Reptilia NKAIN46
Na+/K+ transporting ATPase interacting 4
74(a)
1 ↔ 1
4(153290459-153419805)
African clawed frog
(Xenopus laevis)
Amphibia CD328764.12   -- 77.3(n)    CD328764.1 
zebrafish
(Danio rerio)
Actinopterygii nkain41 Na+/K+ transporting ATPase interacting 4 71.08(n)
70.59(a)
  100002876  NM_001113646.1  NP_001107118.1 
fruit fly
(Drosophila melanogaster)
Insecta NKAIN6
Na,K-ATPase Interacting
12(a)
1 → many
2R(20769075-20776138)


ENSEMBL Gene Tree for NKAIN4 (if available)
TreeFam Gene Tree for NKAIN4 (if available)

(Paralogs according to 1HomoloGene,
2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
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Paralogs for NKAIN4 gene
NKAIN12  NKAIN22  NKAIN32  
4 SIMAP similar genes for NKAIN4 using alignment to 4 protein entries:     NKAI4_HUMAN (see all proteins):
NKAIN3    NKAIN2    NKAIN1    TCBA1

Find genes that share paralogs with NKAIN4           About GenesLikeMe



(SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
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Selected SNPs for NKAIN4 (see all 362)    About this table                                 

Genomic DataTranscription Related DataAllele Frequencies
SNP IDValidClinical
significance
Chr 20 posSequence#AA
Chg
TypeMore#Allele
freq
PopTotal
sample
More
----------
rs801336701,2
C,F--61872401(+) GCCCTC/TACCGC 1 -- ut311Minor allele frequency- T:0.01WA 118
rs733160341,2
C,F--61872450(+) GCTGCA/TTCCCA 1 -- ut313Minor allele frequency- T:0.08WA CSA 122
rs60898671,2
--61872548(+) GAGCTG/TTGACC 1 -- ut31 ese30--------
rs1415660421,2
--61872603(+) TTTTGC/TATGTT 1 -- ut310--------
rs1470532441,2
--61872659(+) TTGACA/GCTGCA 1 -- ut310--------
rs1382370191,2
--61872762(+) GGGCGC/TGTCCC 1 -- ut310--------
rs2018703371,2
C--61872883(+) GAGCAC/TCTGGG 1 -- int10--------
rs10478261,2
C,F,A,H--61872905(-) GTGGCC/TGCCCC 1 -- int114Minor allele frequency- T:0.25EA NS NA WA CSA 554
rs1151469061,2
F--61872917(+) GGGGGC/ATGCCA 1 -- int11Minor allele frequency- A:0.01WA 118
rs1876330871,2
--61872964(+) CCAAGC/TAGTGG 1 -- int10--------

HapMap Linkage Disequilibrium report for NKAIN4 (61872136 - 61904046 bp)

Structural Variations
     Database of Genomic Variants (DGV) Selected variations for NKAIN4 (see all 29):    About this table    
Variant IDTypeSubtypePubMed ID
esv2722898CNV Deletion23290073
nsv522880CNV Loss19592680
dgv4627n71CNV Loss21882294
nsv913165CNV Loss21882294
dgv4618n71CNV Loss21882294
nsv517648CNV Loss19592680
nsv834031CNV Loss17160897
dgv4626n71CNV Loss21882294
nsv3446CNV Loss18451855
dgv4629n71CNV Loss21882294

Site Specific Mutation Identification with PCR Assays
SeqTarget long-range PCR primers for resequencing NKAIN4
DNA2.0 Custom Variant and Variant Library Synthesis for NKAIN4

(in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
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OMIM gene information: 612873    OMIM disorders: --


Find genes that share disorders with NKAIN4           About GenesLikeMe


Export disorders for NKAIN4 gene to outside databases

(in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
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PubMed articles for NKAIN4 gene integrated from 10 sources:
(articles sorted by number of sources associating them with NKAIN4)
    Utopia: connect your pdf to the dynamic
world of online information

  1. A novel family of transmembrane proteins interacting with beta subunits of the Na,K-ATPase. (PubMed id 17606467)1, 3 Gorokhova S....Heintz N. (Hum. Mol. Genet. 2007)
  2. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S.... Malek J. (Genome Res. 2004)
  3. The DNA sequence and comparative analysis of human chromosome 20. (PubMed id 11780052)1, 2 Deloukas P....Rogers J. (Nature 2001)
  4. hORFeome v3.1: a resource of human open reading frames representing over 10,000 human genes. (PubMed id 17207965)1 Lamesch P.... Vidal M. (Genomics 2007)
  5. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. (PubMed id 12477932)1 Strausberg R.L....Marra M.A. (Proc. Natl. Acad. Sci. U.S.A. 2002)
  6. Normalization and subtraction: two approaches to facilitate gene discovery. (PubMed id 8889548)1 Bonaldo M.F.... Soares M.B. (Genome Res. 1996)
  7. Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides. (PubMed id 8125298)1 Maruyama K. and Sugano S. (Gene 1994)

(in PubMed, OMIM, and NCBI Bookshelf)
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 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
  (Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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Entrez Gene: 128414 HGNC: 16191 AceView: C20orf58 Ensembl:ENSG00000101198 euGenes: HUgn128414
ECgene: NKAIN4 H-InvDB: NKAIN4

(According to HUGE)
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  --

(According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
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NameDescription
PharmGKB entry for NKAIN4 Pharmacogenomics, SNPs, Pathways

(Patent information from GeneIP,
Licensable technologies from WIS Yeda, Salk, Tufts,
IP news from LifeMap Sciences, Inc.)
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Patent Information for NKAIN4 gene:
Search GeneIP for patents involving NKAIN4

GeneCards and IP:
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(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, eBioscience, antibodies-online, and/or others, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Addgene, Cell lines from GenScript, and ESI BIO, Flow cytometery from eBioscience, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
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