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Aliases for NIT2 Gene

Aliases for NIT2 Gene

  • Nitrilase Family Member 2 2 3 5
  • Nitrilase Homolog 2 3 4
  • Omega-Amidase NIT2 2 3
  • Epididymis Secretory Sperm Binding Protein Li 8a 3
  • Nitrilase Family, Member 2 2
  • Nit Protein 2 3
  • EC 3.5.1.3 4
  • HEL-S-8a 3

External Ids for NIT2 Gene

Previous GeneCards Identifiers for NIT2 Gene

  • GC03M096559
  • GC03P099332
  • GC03P101334
  • GC03P101374
  • GC03P101536
  • GC03P100053
  • GC03P097421

Summaries for NIT2 Gene

GeneCards Summary for NIT2 Gene

NIT2 (Nitrilase Family Member 2) is a Protein Coding gene. Among its related pathways are Innate Immune System and Alanine, aspartate and glutamate metabolism. GO annotations related to this gene include hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds and omega-amidase activity.

UniProtKB/Swiss-Prot for NIT2 Gene

  • Has a omega-amidase activity. The role of omega-amidase is to remove potentially toxic intermediates by converting alpha-ketoglutaramate and alpha-ketosuccinamate to biologically useful alpha-ketoglutarate and oxaloacetate, respectively. Overexpression decreases the colony-forming capacity of cultured cells by arresting cells in the G2 phase of the cell cycle.

No data available for Entrez Gene Summary , CIViC summary , Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for NIT2 Gene

Genomics for NIT2 Gene

Regulatory Elements for NIT2 Gene

Enhancers for NIT2 Gene
GeneHancer Identifier Enhancer Score Enhancer Sources Gene-Enhancer Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor Binding Sites within enhancer Gene Targets for Enhancer
GH03G100333 1.1 ENCODE 13.5 +0.7 694 3.4 HDGF PKNOX1 FOXA2 ARNT ARID4B SIN3A DMAP1 ZNF2 YY1 ZNF143 LNP1 TOMM70 TBC1D23 NIT2
GH03G100360 0.5 FANTOM5 12 +25.8 25756 0.2 ZNF316 MAFG EMSY MAFK NIT2 LNP1 TOMM70
GH03G100123 1 ENCODE dbSUPER 5.2 -210.1 -210145 2.8 CTCF ZNF146 FOXA2 RAD21 TEAD3 SMC3 FOS GATAD2A ZNF654 TEAD1 FILIP1L TMEM45A NIT2 GC03M100139
GH03G100359 0.3 FANTOM5 12.1 +24.5 24546 0.2 NIT2 LNP1 TOMM70 FILIP1L
GH03G100309 0.3 FANTOM5 11.9 -25.3 -25262 0.4 NIT2 TBC1D23 LNP1 TOMM70 TFG
- Elite enhancer and/or Elite enhancer-gene association Download GeneHancer data dump

Enhancers around NIT2 on UCSC Golden Path with GeneCards custom track

Promoters for NIT2 Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters
ENSR00000155632 299 1201 HDGF PKNOX1 FOXA2 ARNT ARID4B SIN3A DMAP1 ZNF2 YY1 ZNF143

Genomic Location for NIT2 Gene

Chromosome:
3
Start:
100,334,701 bp from pter
End:
100,361,635 bp from pter
Size:
26,935 bases
Orientation:
Plus strand

Genomic View for NIT2 Gene

Genes around NIT2 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
NIT2 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for NIT2 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for NIT2 Gene

Proteins for NIT2 Gene

  • Protein details for NIT2 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q9NQR4-NIT2_HUMAN
    Recommended name:
    Omega-amidase NIT2
    Protein Accession:
    Q9NQR4
    Secondary Accessions:
    • B2R9A3
    • D3DN47
    • Q8WUF0

    Protein attributes for NIT2 Gene

    Size:
    276 amino acids
    Molecular mass:
    30608 Da
    Quaternary structure:
    • Homodimer.

neXtProt entry for NIT2 Gene

Post-translational modifications for NIT2 Gene

  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

Other Protein References for NIT2 Gene

No data available for DME Specific Peptides for NIT2 Gene

Domains & Families for NIT2 Gene

Protein Domains for NIT2 Gene

Suggested Antigen Peptide Sequences for NIT2 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

Q9NQR4

UniProtKB/Swiss-Prot:

NIT2_HUMAN :
  • Belongs to the carbon-nitrogen hydrolase superfamily. NIT1/NIT2 family.
Family:
  • Belongs to the carbon-nitrogen hydrolase superfamily. NIT1/NIT2 family.
genes like me logo Genes that share domains with NIT2: view

No data available for Gene Families for NIT2 Gene

Function for NIT2 Gene

Molecular function for NIT2 Gene

UniProtKB/Swiss-Prot BiophysicochemicalProperties:
Kinetic parameters: KM=0.009 mM for alpha-ketoglutaramate (open-chain form) {ECO:0000269 PubMed:19595734}; Vmax=5.9 umol/min/mg enzyme with alpha-ketoglutaramate as substrate {ECO:0000269 PubMed:19595734}; Note=In solution, alpha-ketoglutaramate is in equilibrium with a cyclic form (2-hydroxy-5-oxoproline), and at pH 8.0 or above, the rate of ring opening is no longer limiting for the omega- amidase reaction.;
UniProtKB/Swiss-Prot CatalyticActivity:
A monoamide of a dicarboxylate + H(2)O = a dicarboxylate + NH(3).
UniProtKB/Swiss-Prot Function:
Has a omega-amidase activity. The role of omega-amidase is to remove potentially toxic intermediates by converting alpha-ketoglutaramate and alpha-ketosuccinamate to biologically useful alpha-ketoglutarate and oxaloacetate, respectively. Overexpression decreases the colony-forming capacity of cultured cells by arresting cells in the G2 phase of the cell cycle.

Enzyme Numbers (IUBMB) for NIT2 Gene

Gene Ontology (GO) - Molecular Function for NIT2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0016787 hydrolase activity IEA --
GO:0016810 hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds IEA --
GO:0050152 omega-amidase activity IDA 22674578
genes like me logo Genes that share ontologies with NIT2: view
genes like me logo Genes that share phenotypes with NIT2: view

Animal Model Products

  • Taconic Biosciences Mouse Models for NIT2

CRISPR Products

miRNA for NIT2 Gene

miRTarBase miRNAs that target NIT2

Inhibitory RNA Products

Clone Products

No data available for Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for NIT2 Gene

Localization for NIT2 Gene

Subcellular locations from UniProtKB/Swiss-Prot for NIT2 Gene

Cytoplasm.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for NIT2 gene
Compartment Confidence
extracellular 5
cytoskeleton 5
cytosol 5
mitochondrion 4
nucleus 2
peroxisome 1

Gene Ontology (GO) - Cellular Components for NIT2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005576 extracellular region TAS --
GO:0005737 cytoplasm IEA --
GO:0005739 mitochondrion IEA --
GO:0005813 centrosome IDA --
GO:0005829 cytosol IDA --
genes like me logo Genes that share ontologies with NIT2: view

Pathways & Interactions for NIT2 Gene

genes like me logo Genes that share pathways with NIT2: view

Pathways by source for NIT2 Gene

3 Reactome pathways for NIT2 Gene
1 KEGG pathway for NIT2 Gene

Gene Ontology (GO) - Biological Process for NIT2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006107 oxaloacetate metabolic process IDA 22674578
GO:0006528 asparagine metabolic process IDA 22674578
GO:0006541 glutamine metabolic process IDA 22674578
GO:0006807 nitrogen compound metabolic process IEA --
GO:0043312 neutrophil degranulation TAS --
genes like me logo Genes that share ontologies with NIT2: view

No data available for SIGNOR curated interactions for NIT2 Gene

Drugs & Compounds for NIT2 Gene

(1) Drugs for NIT2 Gene - From: HMDB

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Water Approved Pharma 0

(5) Additional Compounds for NIT2 Gene - From: HMDB

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
2-Keto-glutaramic acid
  • 2-Keto-glutaramate
  • 2-Oxoglutaramate
  • alpha-Ketoglutaramate
18465-19-5
2-Oxosuccinamate
Ammonia
  • Ammonia anhydrous
  • Ammonia inhalant
  • Ammonia solution strong [usan]
  • Ammonia water
  • Ammoniak
7664-41-7
Oxalacetic acid
  • 2-Ketosuccinate
  • 2-Ketosuccinic acid
  • 2-Oxobutanedioate
  • 2-Oxobutanedioic acid
  • 2-Oxosuccinate
328-42-7
Oxoglutaric acid
  • 2-Ketoglutarate
  • 2-Ketoglutaric acid
  • 2-Oxo-1,5-pentanedioate
  • 2-Oxo-1,5-pentanedioic acid
  • 2-Oxoglutarate
328-50-7
genes like me logo Genes that share compounds with NIT2: view

Transcripts for NIT2 Gene

Unigene Clusters for NIT2 Gene

Nitrilase family, member 2:
Representative Sequences:

CRISPR Products

Inhibitory RNA Products

Clone Products

Alternative Splicing Database (ASD) splice patterns (SP) for NIT2 Gene

ExUns: 1a · 1b ^ 2a · 2b ^ 3a · 3b ^ 4a · 4b ^ 5 ^ 6 ^ 7a · 7b · 7c ^ 8 ^ 9 ^ 10 ^ 11
SP1: - - -
SP2: - -
SP3:
SP4:
SP5:
SP6:

Relevant External Links for NIT2 Gene

GeneLoc Exon Structure for
NIT2
ECgene alternative splicing isoforms for
NIT2

Expression for NIT2 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and CGAP SAGE for NIT2 Gene

mRNA differential expression in normal tissues according to GTEx for NIT2 Gene

This gene is overexpressed in Liver (x4.5).

Protein differential expression in normal tissues from HIPED for NIT2 Gene

This gene is overexpressed in Liver, secretome (18.9).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MaxQB, and MOPED for NIT2 Gene



Protein tissue co-expression partners for NIT2 Gene

- Elite partner

NURSA nuclear receptor signaling pathways regulating expression of NIT2 Gene:

NIT2

SOURCE GeneReport for Unigene cluster for NIT2 Gene:

Hs.439152

mRNA Expression by UniProt/SwissProt for NIT2 Gene:

Q9NQR4-NIT2_HUMAN
Tissue specificity: Detected in fetal brain (at protein level). Ubiquitous. Detected in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, prostate, spleen, thymus, prostate, testis, ovary, small intestine and colon.

Evidence on tissue expression from TISSUES for NIT2 Gene

  • Liver(4.4)
genes like me logo Genes that share expression patterns with NIT2: view

Primer Products

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery and Phenotype-based relationships between genes and organs from Gene ORGANizer for NIT2 Gene

Orthologs for NIT2 Gene

This gene was present in the common ancestor of eukaryotes.

Orthologs for NIT2 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia NIT2 34 35
  • 99.27 (n)
dog
(Canis familiaris)
Mammalia NIT2 34 35
  • 89.25 (n)
cow
(Bos Taurus)
Mammalia NIT2 34 35
  • 89.09 (n)
rat
(Rattus norvegicus)
Mammalia Nit2 34
  • 87.92 (n)
mouse
(Mus musculus)
Mammalia Nit2 34 16 35
  • 87.68 (n)
oppossum
(Monodelphis domestica)
Mammalia NIT2 35
  • 77 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia NIT2 35
  • 69 (a)
OneToOne
chicken
(Gallus gallus)
Aves NIT2 34 35
  • 76.85 (n)
lizard
(Anolis carolinensis)
Reptilia NIT2 35
  • 75 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia nit2 34
  • 72.36 (n)
Str.8728 34
African clawed frog
(Xenopus laevis)
Amphibia Xl.4774 34
zebrafish
(Danio rerio)
Actinopterygii nit2 34 35
  • 69 (n)
Dr.27178 34
fruit fly
(Drosophila melanogaster)
Insecta CG8132 36 34 35
  • 57.81 (n)
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP012801 34
  • 57.25 (n)
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes NIT3 34 35
  • 53.68 (n)
K. lactis yeast
(Kluyveromyces lactis)
Saccharomycetes KLLA0A02453g 34
  • 51.24 (n)
A. gosspyii yeast
(Ashbya gossypii)
Saccharomycetes AGOS_ACL190W 34
  • 50.69 (n)
thale cress
(Arabidopsis thaliana)
eudicotyledons AT5G12040 34
  • 57.07 (n)
rice
(Oryza sativa)
Liliopsida Os03g0175600 34
  • 54.89 (n)
Os.19194 34
wheat
(Triticum aestivum)
Liliopsida Ta.30353 34
corn
(Zea mays)
Liliopsida Zm.3591 34
fission yeast
(Schizosaccharomyces pombe)
Schizosaccharomycetes SPAC26A3.11 34
  • 55.22 (n)
sea squirt
(Ciona savignyi)
Ascidiacea -- 35
  • 55 (a)
OneToOne
bread mold
(Neurospora crassa)
Ascomycetes NCU06726 34
  • 52.82 (n)
sea squirt
(Ciona intestinalis)
Ascidiacea Cin.690 34
Species where no ortholog for NIT2 was found in the sources mined by GeneCards:
  • Actinobacteria (Mycobacterium tuberculosis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • worm (Caenorhabditis elegans)

Evolution for NIT2 Gene

ENSEMBL:
Gene Tree for NIT2 (if available)
TreeFam:
Gene Tree for NIT2 (if available)

Paralogs for NIT2 Gene

(1) SIMAP similar genes for NIT2 Gene using alignment to 4 proteins:

Pseudogenes.org Pseudogenes for NIT2 Gene

genes like me logo Genes that share paralogs with NIT2: view

No data available for Paralogs for NIT2 Gene

Variants for NIT2 Gene

Sequence variations from dbSNP and Humsavar for NIT2 Gene

SNP ID Clin Chr 03 pos Sequence Context AA Info Type
rs1000244018 -- 100,335,156(+) ACCTC(C/G)TCCAG intron-variant
rs1000504748 -- 100,354,075(+) CCTTC(A/C)TGGAG intron-variant
rs1000557352 -- 100,347,073(+) GAGAT(A/C)AATTT intron-variant
rs1000944250 -- 100,334,999(+) TTCCG(A/G)GGTGG intron-variant
rs1000976662 -- 100,335,297(+) GTCCC(C/T)TGTTT intron-variant

Structural Variations from Database of Genomic Variants (DGV) for NIT2 Gene

Variant ID Type Subtype PubMed ID
esv3387925 CNV duplication 20981092
nsv1114411 CNV deletion 24896259

Variation tolerance for NIT2 Gene

Residual Variation Intolerance Score: 23% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 1.09; 22.17% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for NIT2 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
NIT2

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for NIT2 Gene

Disorders for NIT2 Gene

Relevant External Links for NIT2

Atlas of Genetics and Cytogenetics in Oncology and Haematology:
NIT2

No disorders were found for NIT2 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for NIT2 Gene

Publications for NIT2 Gene

  1. Growth inhibitory effect of the human NIT2 gene and its allelic imbalance in cancers. (PMID: 17488281) Lin C.-H. … Chien C.-H. (FEBS J. 2007) 3 4 22 64
  2. Crystal structure of the worm NitFhit Rosetta stone protein reveals a Nit tetramer binding two Fhit dimers. (PMID: 10959838) Pace H.C. … Brenner C. (Curr. Biol. 2000) 2 3 4 64
  3. Mitochondrial Protein Interaction Mapping Identifies Regulators of Respiratory Chain Function. (PMID: 27499296) Floyd B.J. … Pagliarini D.J. (Mol. Cell 2016) 3 64
  4. Proteomic analyses reveal distinct chromatin-associated and soluble transcription factor complexes. (PMID: 25609649) Li X. … Chen J. (Mol. Syst. Biol. 2015) 3 64
  5. Downregulation of NIT2 inhibits colon cancer cell proliferation and induces cell cycle arrest through the caspase-3 and PARP pathways. (PMID: 25738796) Zheng B. … Yu X. (Int. J. Mol. Med. 2015) 3 64

Products for NIT2 Gene

Sources for NIT2 Gene

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