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Aliases for NIT1 Gene

Aliases for NIT1 Gene

  • Nitrilase 1 2 3 5
  • EC 3.5.-.- 4

External Ids for NIT1 Gene

Previous GeneCards Identifiers for NIT1 Gene

  • GC01P158799
  • GC01P156820
  • GC01P157865
  • GC01P158304
  • GC01P157900
  • GC01P159355
  • GC01P161087
  • GC01P132445

Summaries for NIT1 Gene

Entrez Gene Summary for NIT1 Gene

  • This gene encodes a member of the nitrilase protein family with homology to bacterial and plant nitrilases, enzymes that cleave nitriles and organic amides to the corresponding carboxylic acids plus ammonia. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]

GeneCards Summary for NIT1 Gene

NIT1 (Nitrilase 1) is a Protein Coding gene. GO annotations related to this gene include hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds and nitrilase activity.

UniProtKB/Swiss-Prot for NIT1 Gene

  • Plays a role in cell growth and apoptosis: loss of expression promotes cell growth and resistance to DNA damage stress. Has tumor suppressor properties that enhances the apoptotic responsiveness in cancer cells; this effect is additive to the tumor suppressor activity of FHIT. It is also a negative regulator of primary T-cells. Has apparently no omega-amidase activity such as NIT2 (By similarity).

No data available for Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for NIT1 Gene

Genomics for NIT1 Gene

Regulatory Elements for NIT1 Gene

Enhancers for NIT1 Gene
GeneHancer Identifier Enhancer Score Enhancer Sources Gene-Enhancer Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor Binding Sites within enhancer Gene Targets for Enhancer
GH01F161113 1.1 Ensembl ENCODE 10 +0.2 193 8.6 HDGF PKNOX1 CREB3L1 WRNIP1 ARID4B SIN3A FEZF1 DMAP1 ZNF2 YY1 B4GALT3 NIT1 F11R HSPA6 PIR38070 PIR41247
GH01F161158 0.2 ENCODE 7.8 +42.4 42426 4.9 HDGF ARNT SIN3A ARID4B DMAP1 YBX1 ZNF2 YY1 ZNF766 ZNF143 DUSP12 ENSG00000228606 ENSG00000238934 ENSG00000224985 ENSG00000227818 ATF6 NIT1 TSTD1 USP21 PPOX
GH01F161196 1.2 FANTOM5 ENCODE 7.2 +82.0 81999 8.0 MLX CREB3L1 DMAP1 YY1 ZNF143 ZNF263 SP3 NFYC SSRP1 ZNF610 B4GALT3 NDUFS2 ARHGAP30 CD48 DUSP12 ADAMTS4 NIT1 TOMM40L PCP4L1 FCER1G
GH01F161175 0.2 ENCODE 6.9 +58.8 58818 3.2 HDGF PKNOX1 ARNT CREB3L1 MLX ZFP64 ARID4B SIN3A FEZF1 DMAP1 NIT1 B4GALT3 PPOX
GH01F161180 1 FANTOM5 Ensembl ENCODE 6.9 +66.8 66830 9.4 HDGF PKNOX1 ARNT CREB3L1 ZFP64 ARID4B SIN3A DMAP1 ZNF2 YY1 B4GALT3 ADAMTS4 SLAMF7 NIT1 PPOX FCER1G CD48 ARHGAP30 CD244 FCGR3A
- Elite enhancer/Elite enhancer-gene association Download Table
Download GeneHancer data dump

Enhancers around NIT1 on UCSC Golden Path with GeneCards custom track

Promoters for NIT1 Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters
ENSR00000546413 -272 2001 HDGF CREB3L1 WRNIP1 ARID4B SIN3A FEZF1 DMAP1 YY1 FOS KLF13

Genomic Location for NIT1 Gene

Chromosome:
1
Start:
161,118,072 bp from pter
End:
161,125,445 bp from pter
Size:
7,374 bases
Orientation:
Plus strand

Genomic View for NIT1 Gene

Genes around NIT1 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
NIT1 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for NIT1 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for NIT1 Gene

Proteins for NIT1 Gene

  • Protein details for NIT1 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q86X76-NIT1_HUMAN
    Recommended name:
    Nitrilase homolog 1
    Protein Accession:
    Q86X76
    Secondary Accessions:
    • B1AQP3
    • D3DVF4
    • O76091

    Protein attributes for NIT1 Gene

    Size:
    327 amino acids
    Molecular mass:
    35896 Da
    Quaternary structure:
    No Data Available
    Miscellaneous:
    • According to Rosetta Stone theory, the existence of a fusion protein in one genome predicts that the separate polypeptides expressed in other organisms function in the same cellular or biochemical pathway. In Drosophila melanogaster and Caenorhabditis elegans, NitFhit is a fusion protein composed of a C-terminal Fhit domain and a domain related to plant and bacterial nitrilase.

    Alternative splice isoforms for NIT1 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for NIT1 Gene

Post-translational modifications for NIT1 Gene

  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

No data available for DME Specific Peptides for NIT1 Gene

Domains & Families for NIT1 Gene

Suggested Antigen Peptide Sequences for NIT1 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

Q86X76

UniProtKB/Swiss-Prot:

NIT1_HUMAN :
  • Contains 1 CN hydrolase domain.
  • Belongs to the carbon-nitrogen hydrolase superfamily. NIT1/NIT2 family.
Domain:
  • Contains 1 CN hydrolase domain.
Family:
  • Belongs to the carbon-nitrogen hydrolase superfamily. NIT1/NIT2 family.
genes like me logo Genes that share domains with NIT1: view

No data available for Gene Families for NIT1 Gene

Function for NIT1 Gene

Molecular function for NIT1 Gene

UniProtKB/Swiss-Prot Function:
Plays a role in cell growth and apoptosis: loss of expression promotes cell growth and resistance to DNA damage stress. Has tumor suppressor properties that enhances the apoptotic responsiveness in cancer cells; this effect is additive to the tumor suppressor activity of FHIT. It is also a negative regulator of primary T-cells. Has apparently no omega-amidase activity such as NIT2 (By similarity).

Enzyme Numbers (IUBMB) for NIT1 Gene

Gene Ontology (GO) - Molecular Function for NIT1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000257 nitrilase activity TAS 9671749
GO:0016787 hydrolase activity IEA --
GO:0016810 hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds IEA --
genes like me logo Genes that share ontologies with NIT1: view
genes like me logo Genes that share phenotypes with NIT1: view

Animal Models for NIT1 Gene

MGI Knock Outs for NIT1:

Animal Model Products

  • Taconic Biosciences Mouse Models for NIT1

miRNA for NIT1 Gene

miRTarBase miRNAs that target NIT1

Flow Cytometry Products

No data available for Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for NIT1 Gene

Localization for NIT1 Gene

Subcellular locations from UniProtKB/Swiss-Prot for NIT1 Gene

Cytoplasm. Mitochondrion.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for NIT1 Gene COMPARTMENTS Subcellular localization image for NIT1 gene
Compartment Confidence
extracellular 5
mitochondrion 5
nucleus 5
cytosol 3
endoplasmic reticulum 1
lysosome 1
plasma membrane 1
vacuole 1

Gene Ontology (GO) - Cellular Components for NIT1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus IDA 21630459
GO:0005737 cytoplasm IEA --
GO:0005739 mitochondrion IEA --
GO:0070062 extracellular exosome IDA 23376485
genes like me logo Genes that share ontologies with NIT1: view

Pathways & Interactions for NIT1 Gene

SuperPathways for NIT1 Gene

No Data Available

Interacting Proteins for NIT1 Gene

Gene Ontology (GO) - Biological Process for NIT1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006807 nitrogen compound metabolic process IEA --
GO:0008150 biological_process ND --
genes like me logo Genes that share ontologies with NIT1: view

No data available for Pathways by source and SIGNOR curated interactions for NIT1 Gene

Transcripts for NIT1 Gene

Unigene Clusters for NIT1 Gene

Nitrilase 1:
Representative Sequences:

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for NIT1 Gene

ExUns: 1a · 1b · 1c · 1d · 1e ^ 2 ^ 3a · 3b · 3c ^ 4a · 4b · 4c ^ 5a · 5b ^ 6a · 6b · 6c · 6d ^ 7a · 7b ^ 8a · 8b
SP1: - - - - - -
SP2: - - - -
SP3: - - - - - - -
SP4: -
SP5: - - - - - - - -
SP6: - - -
SP7: - - - - -
SP8: - - - - - - - -
SP9: - -
SP10:

Relevant External Links for NIT1 Gene

GeneLoc Exon Structure for
NIT1
ECgene alternative splicing isoforms for
NIT1

Expression for NIT1 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for NIT1 Gene

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MaxQB, and MOPED for NIT1 Gene



Protein tissue co-expression partners for NIT1 Gene

- Elite partner

NURSA nuclear receptor signaling pathways regulating expression of NIT1 Gene:

NIT1

SOURCE GeneReport for Unigene cluster for NIT1 Gene:

Hs.146406

mRNA Expression by UniProt/SwissProt for NIT1 Gene:

Q86X76-NIT1_HUMAN
Tissue specificity: Detected in heart, brain, placenta, liver, skeletal muscle, kidney and pancreas.
genes like me logo Genes that share expression patterns with NIT1: view

Primer Products

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery , mRNA differential expression in normal tissues and Protein differential expression in normal tissues for NIT1 Gene

Orthologs for NIT1 Gene

This gene was present in the common ancestor of eukaryotes.

Orthologs for NIT1 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia NIT1 34 35
  • 99.39 (n)
dog
(Canis familiaris)
Mammalia NIT1 34 35
  • 91.95 (n)
cow
(Bos Taurus)
Mammalia NIT1 34 35
  • 91.85 (n)
rat
(Rattus norvegicus)
Mammalia Nit1 34
  • 87.58 (n)
mouse
(Mus musculus)
Mammalia Nit1 34 16 35
  • 87.1 (n)
oppossum
(Monodelphis domestica)
Mammalia NIT1 35
  • 72 (a)
OneToOne
chicken
(Gallus gallus)
Aves NIT1 34 35
  • 66.34 (n)
tropical clawed frog
(Silurana tropicalis)
Amphibia nit1 34
  • 64.53 (n)
African clawed frog
(Xenopus laevis)
Amphibia Nit1 34
zebrafish
(Danio rerio)
Actinopterygii nit1 34 35
  • 58.07 (n)
Dr.16022 34
fruit fly
(Drosophila melanogaster)
Insecta NitFhit 36 35
  • 48 (a)
worm
(Caenorhabditis elegans)
Secernentea nft-1 36 35
  • 50 (a)
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes NIT2 34 35 37
  • 47.17 (n)
K. lactis yeast
(Kluyveromyces lactis)
Saccharomycetes KLLA0E15247g 34
  • 44.89 (n)
thale cress
(Arabidopsis thaliana)
eudicotyledons AT4G08790 34
  • 52.75 (n)
rice
(Oryza sativa)
Liliopsida Os12g0502500 34
  • 51.72 (n)
fission yeast
(Schizosaccharomyces pombe)
Schizosaccharomycetes SPBC651.02 34
  • 50.75 (n)
bread mold
(Neurospora crassa)
Ascomycetes NCU05757 34
  • 50.18 (n)
sea squirt
(Ciona savignyi)
Ascidiacea -- 35
  • 47 (a)
OneToOne
Species where no ortholog for NIT1 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • lizard (Anolis carolinensis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • platypus (Ornithorhynchus anatinus)
  • rainbow trout (Oncorhynchus mykiss)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for NIT1 Gene

ENSEMBL:
Gene Tree for NIT1 (if available)
TreeFam:
Gene Tree for NIT1 (if available)

Paralogs for NIT1 Gene

(1) SIMAP similar genes for NIT1 Gene using alignment to 1 proteins:

genes like me logo Genes that share paralogs with NIT1: view

No data available for Paralogs for NIT1 Gene

Variants for NIT1 Gene

Sequence variations from dbSNP and Humsavar for NIT1 Gene

SNP ID Clin Chr 01 pos Sequence Context AA Info Type
rs10908823 -- 161,125,044(+) TGAGC(C/T)ATAAT intron-variant, utr-variant-3-prime
rs111413504 -- 161,119,106(+) GAAAT(A/C/G)TGAGA intron-variant, upstream-variant-2KB
rs111473243 -- 161,119,660(+) CTACC(-/TAAACT)TAGAT intron-variant, upstream-variant-2KB
rs111698948 -- 161,116,820(+) TCCCG(C/T)AACTG intron-variant, upstream-variant-2KB
rs112277456 -- 161,124,920(+) CAAGA(-/C)CCCCA intron-variant, utr-variant-3-prime

Variation tolerance for NIT1 Gene

Residual Variation Intolerance Score: 28.3% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 6.80; 79.09% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for NIT1 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
NIT1

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot and Structural Variations from Database of Genomic Variants (DGV) for NIT1 Gene

Disorders for NIT1 Gene

Relevant External Links for NIT1

Genetic Association Database (GAD)
NIT1
Human Genome Epidemiology (HuGE) Navigator
NIT1
Atlas of Genetics and Cytogenetics in Oncology and Haematology:
NIT1

No disorders were found for NIT1 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for NIT1 Gene

Publications for NIT1 Gene

  1. Nitrilase and Fhit homologs are encoded as fusion proteins in Drosophila melanogaster and Caenorhabditis elegans. (PMID: 9671749) Pekarsky Y. … Croce C.M. (Proc. Natl. Acad. Sci. U.S.A. 1998) 2 3 4 22 64
  2. Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression. (PMID: 20877624) Hendrickson S.L. … O'Brien S.J. (PLoS ONE 2010) 3 46 64
  3. Biological functions of mammalian Nit1, the counterpart of the invertebrate NitFhit Rosetta stone protein, a possible tumor suppressor. (PMID: 16864578) Semba S. … Huebner K. (J. Biol. Chem. 2006) 3 22 64
  4. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PMID: 15489334) Gerhard D.S. … Malek J. (Genome Res. 2004) 3 4 64
  5. The scaffold protein KSR1, a novel therapeutic target for the treatment of Merlin-deficient tumors. (PMID: 26549023) Zhou L. … Hanemann C.O. (Oncogene 2016) 3 64

Products for NIT1 Gene

Sources for NIT1 Gene

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