Set Analyses:
Advanced Search

Advanced Search

 
Search By
Section (entire)
for


 
or upload a file of gene symbols


Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

NISCH Gene

protein-coding   GIFtS: 62
GCID: GC03P052489

Nischarin

Alzheimer's & Parkinson's Diseases Congress
  See related diseases
at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
About This Section

Aliases
nischarin1 2     I1R Candidate Protein2 3
I-1 Receptor Candidate Protein1 2 3     Imidazoline Receptor Candidate1
Imidazoline Receptor Antisera Selected1 2     I1R3
Imidazoline Receptor 12 3     KIAA09753
I-12 3     Imidazoline Receptor Antisera-Selected Protein3
IR12 3     Imidazoline-1 Receptor3
IRAS2 3     Imidazoline-1 Receptor Candidate Protein3
hIRAS2 3     

External Ids:    HGNC: 180061   Entrez Gene: 111882   Ensembl: ENSG000000103227   UniProtKB: Q9Y2I13   

Export aliases for NISCH gene to outside databases

Previous GC identifers: GC03P051581 GC03P051741 GC03P052345 GC03P052447 GC03P052464


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for NISCH Gene:
This gene encodes a nonadrenergic imidazoline-1 receptor protein that localizes to the cytosol and anchors to the
inner layer of the plasma membrane. The orthologous mouse protein has been shown to influence cytoskeletal
organization and cell migration by binding to alpha-5-beta-1 integrin. In humans, this protein has been shown to
bind to the adapter insulin receptor substrate 4 (IRS4) to mediate translocation of alpha-5 integrin from the
cell membrane to endosomes. Expression of this protein was reduced in human breast cancers while its
overexpression reduced tumor growth and metastasis; possibly by limiting the expression of alpha-5 integrin. In
human cardiac tissue, this gene was found to affect cell growth and death while in neural tissue it affected
neuronal growth and differentiation. Alternative splicing results in multiple transcript variants encoding
differerent isoforms. Some isoforms lack the expected C-terminal domains of a functional imidazoline receptor.
(provided by RefSeq, Jan 2013)

GeneCards Summary for NISCH Gene: 
NISCH (nischarin) is a protein-coding gene. Diseases associated with NISCH include rosacea, and morphine dependence, and among its related super-pathways are Cell adhesion Integrin-mediated cell adhesion and migration. GO annotations related to this gene include integrin binding and G-protein coupled amine receptor activity. An important paralog of this gene is STK11IP.

UniProtKB/Swiss-Prot: NISCH_HUMAN, Q9Y2I1
Function: Acts either as the functional imidazoline-1 receptor (I1R) candidate or as a membrane-associated
mediator of the I1R signaling. Binds numerous imidazoline ligands that induces initiation of cell-signaling
cascades triggering to cell survival, growth and migration. Its activation by the agonist rilmenidine induces an
increase in phosphorylation of mitogen-activated protein kinases MAPK1 and MAPK3 in rostral ventrolateral medulla
(RVLM) neurons that exhibited rilmenidine-evoked hypotension (By similarity). Blocking its activation with
efaroxan abolished rilmenidine-induced mitogen-activated protein kinase phosphorylation in RVLM neurons (By
similarity). Acts as a modulator of Rac-regulated signal transduction pathways (By similarity). Suppresses
Rac1-stimulated cell migration by interacting with PAK1 and inhibiting its kinase activity (By similarity). Also
blocks Pak-independent Rac signaling by interacting with RAC1 and inhibiting Rac1-stimulated NF-kB response
element and cyclin D1 promoter activation (By similarity). Inhibits also LIMK1 kinase activity by reducing LIMK1
'Tyr-508' phosphorylation (By similarity). Inhibits Rac-induced cell migration and invasion in breast and colon
epithelial cells (By similarity). Inhibits lamellipodia formation, when overexpressed (By similarity). Plays a
role in protection against apoptosis. Involved in association with IRS4 in the enhancement of insulin activation
of MAPK1 and MAPK3. When overexpressed, induces a redistribution of cell surface ITGA5 integrin to intracellular
endosomal structures

Gene Wiki entry for NISCH Gene


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 73), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000003.11  NT_022517.18  NC_018914.2  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the NISCH gene promoter:
         Max1   Sp1   COUP   Olf-1   COUP-TF   Pax-4a   ATF   c-Myc   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidNISCH promoter sequence
   Search SABiosciences Chromatin IP Primers for NISCH

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat NISCH


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 3p21.1   Ensembl cytogenetic band:  3p21.1   HGNC cytogenetic band: 3p21.1

NISCH Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
NISCH gene location

GeneLoc information about chromosome 3         GeneLoc Exon Structure

GeneLoc location for GC03P052489:  view genomic region     (about GC identifiers)

Start:
52,489,134 bp from pter      End:
52,527,088 bp from pter
Size:
37,955 bases      Orientation:
plus strand

(According to UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MAXQB RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Cloud-Clone Corp.,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Cloud-Clone Corp., Ontologies according to Gene Ontology Consortium 01 Oct 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, and/or Cloud-Clone Corp.)
About This Section

UniProtKB/Swiss-Prot: NISCH_HUMAN, Q9Y2I1 (See protein sequence)
Recommended Name: Nischarin  
Size: 1504 amino acids; 166629 Da
Subunit: Homooligomer. Interacts with GRB2. Interacts with PIK3R1; probably associates with the PI3-kinase
complex. Interacts with IRS4. Found in a complex with ITGA5 and PAK1. Found in a complex with LIMK1 and PAK1.
Interacts with ITGA5 (via cytoplasmic domain); this interaction is direct. Interacts with PAK1 (via kinase
domain); this interaction is direct and is increased upon activation of PAK1 (By similarity). Interacts with
LIMK1 (via PDZ and kinase domain); this interaction is direct (By similarity). Interacts with LIMK2; this
interaction depends on LIMK2 activity (By similarity). Interacts with RAC1 (activated state) (By similarity)
Subcellular location: Cell membrane. Cytoplasm. Early endosome. Recycling endosome. Note=Enriched in the
early/sorting and recycling endosomes. Colocalized in early/sorting endosomes with EEA1 and SNX2 and in recycling
endosomes with transferrin receptor. Detected in the perinuclear region partially associated with punctate
structures (By similarity). Colocalizes with PAK1 in cytoplasm, vesicular structures in the perinuclear area and
membrane ruffles (By similarity). Colocalizes with RAC1 in the cytoplasm and vesicles structures (By similarity).
Colocalized with MAPK1 and MAPK3 in RVLM neurons (By similarity)
Sequence caution: Sequence=BAA76819.1; Type=Erroneous initiation; Note=Translation N-terminally shortened;
1 PDB 3D structure from and Proteopedia for NISCH:
3P0C (3D)    
Secondary accessions: C9J245 Q6PGP3 Q6PIB4 Q7L8M3 Q7Z2X6 Q9UES6 Q9UEU4 Q9UFW3
Alternative splicing: 4 isoforms:  Q9Y2I1-1   Q9Y2I1-2   Q9Y2I1-3   Q9Y2I1-4   

Explore the universe of human proteins at neXtProt for NISCH: NX_Q9Y2I1

Explore proteomics data for NISCH at MOPED 

Post-translational modifications:

  • View modification sites using PhosphoSitePlus
  • View neXtProt modification sites for NX_Q9Y2I1

  • NISCH Protein expression data from MOPED1, PaxDb2 and MAXQB3 :    About this image 

    NISCH Protein Expression
    REFSEQ proteins (3 alternative transcripts): 
    NP_001263222.1  NP_001263223.1  NP_009115.2  

    ENSEMBL proteins: 
     ENSP00000418232   ENSP00000417812   ENSP00000392484   ENSP00000339958  

    Human Recombinant Protein Products for NISCH: 
    Browse Purified and Recombinant Proteins at EMD Millipore
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    OriGene Purified Protein for NISCH
    Browse OriGene Protein Over-expression Lysates
    OriGene MassSpec for NISCH 
    OriGene Custom Protein Services for NISCH
    GenScript Custom Purified and Recombinant Proteins Services for NISCH
    Novus Biologicals NISCH Protein
    Browse Sino Biological Recombinant Proteins
    Browse Sino Biological Cell Lysates 
    Browse ProSpec Recombinant Proteins
    Cloud-Clone Corp. Proteins for NISCH 

    Gene Ontology (GO): 5 cellular component terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005769early endosome IEA--
    GO:0005829cytosol IDA15028622
    GO:0005886plasma membrane IDA15028622
    GO:0016020membrane ----
    GO:0055037recycling endosome IEA--

    NISCH for ontologies           About GeneDecksing



    NISCH Antibody Products: 
    Browse EMD Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Browse R&D Systems for Antibodies
    Browse OriGene Antibodies
    OriGene Custom Antibody Services for NISCH
    GenScript Custom Superior Antibodies Services for NISCH
    Novus Biologicals NISCH Antibodies
    Abcam antibodies for NISCH
    Cloud-Clone Corp. Antibodies for NISCH 
    Search ThermoFisher Antibodies for NISCH
    LSBio Antibodies in human, mouse, rat for NISCH 

    Assay Products for NISCH: 
    Browse Kits and Assays available from EMD Millipore
    OriGene Custom Assay Services for NISCH
    Browse R&D Systems for biochemical assays
    GenScript Custom Assay Services for NISCH
    Browse Enzo Life Sciences for kits & assays
    Cloud-Clone Corp. ELISAs for NISCH 
    Cloud-Clone Corp. CLIAs for NISCH


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section
    2 InterPro protein domains:
     IPR001611 Leu-rich_rpt
     IPR001683 Phox

    Graphical View of Domain Structure for InterPro Entry Q9Y2I1

    ProtoNet protein and cluster: Q9Y2I1

    1 Blocks protein domain: IPB001683 Phox-like

    UniProtKB/Swiss-Prot: NISCH_HUMAN, Q9Y2I1
    Domain: Both the presence of the PX domain and the coiled coil region are necessary for its endosomal targeting
    Similarity: Contains 6 LRR (leucine-rich) repeats
    Similarity: Contains 1 PX (phox homology) domain


    NISCH for domains           About GeneDecksing


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    bound targets from SABiosciences, miRNA Gene Targets from miRTarBase, shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Sirion Biotech, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, Vector BioLabs, and Sirion Biotech, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Oct 2013 via Entrez Gene.)
    About This Section

    Molecular Function:

         UniProtKB/Swiss-Prot Summary: NISCH_HUMAN, Q9Y2I1
    Function: Acts either as the functional imidazoline-1 receptor (I1R) candidate or as a membrane-associated
    mediator of the I1R signaling. Binds numerous imidazoline ligands that induces initiation of cell-signaling
    cascades triggering to cell survival, growth and migration. Its activation by the agonist rilmenidine induces an
    increase in phosphorylation of mitogen-activated protein kinases MAPK1 and MAPK3 in rostral ventrolateral medulla
    (RVLM) neurons that exhibited rilmenidine-evoked hypotension (By similarity). Blocking its activation with
    efaroxan abolished rilmenidine-induced mitogen-activated protein kinase phosphorylation in RVLM neurons (By
    similarity). Acts as a modulator of Rac-regulated signal transduction pathways (By similarity). Suppresses
    Rac1-stimulated cell migration by interacting with PAK1 and inhibiting its kinase activity (By similarity). Also
    blocks Pak-independent Rac signaling by interacting with RAC1 and inhibiting Rac1-stimulated NF-kB response
    element and cyclin D1 promoter activation (By similarity). Inhibits also LIMK1 kinase activity by reducing LIMK1
    'Tyr-508' phosphorylation (By similarity). Inhibits Rac-induced cell migration and invasion in breast and colon
    epithelial cells (By similarity). Inhibits lamellipodia formation, when overexpressed (By similarity). Plays a
    role in protection against apoptosis. Involved in association with IRS4 in the enhancement of insulin activation
    of MAPK1 and MAPK3. When overexpressed, induces a redistribution of cell surface ITGA5 integrin to intracellular
    endosomal structures

         Gene Ontology (GO): 4 molecular function terms (GO ID links to tree view):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005178integrin binding IEA--
    GO:0005515protein binding ----
    GO:0008227G-protein coupled amine receptor activity IEA--
    GO:0035091phosphatidylinositol binding IEA--
         
    NISCH for ontologies           About GeneDecksing


    Phenotypes:
         1 MGI phenotypic allele for Nisch (no phenotypes)

    NISCH for phenotypes           About GeneDecksing

    Animal Models:
         MGI mouse knock-out Nischtm1Lex for NISCH

       inGenious Targeting Laboratory - Custom generated mouse model solutions for NISCH 
       inGenious Targeting Laboratory - Custom generated inducible mouse model solutions for NISCH

       genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for NISCH 
       genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for NISCH 

    miRNA
    Products:
        
    QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat NISCH
    8/11 QIAGEN miScript miRNA Assays for microRNAs that regulate NISCH (see all 11):
    hsa-miR-374b* hsa-miR-3673 hsa-miR-15a hsa-miR-503 hsa-miR-497 hsa-miR-646 hsa-miR-424 hsa-miR-16
    SwitchGear 3'UTR luciferase reporter plasmidNISCH 3' UTR sequence
    Inhib. RNA
    Products:
        
    Browse for Gene Knock-down Tools from EMD Millipore
    OriGene RNAi products in human, mouse, rat for NISCH
    QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat NISCH

    Gene Editing
    Products:
    DNA2.0 Custom Protein Engineering Service for NISCH
    Sirion Biotech Customized adenovirus for overexpression of NISCH

    Clone
    Products:
         
    Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore
    OriGene clones in human, mouse for NISCH (see all 7)
    OriGene ORF clones in mouse, rat for NISCH
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector: NISCH (NM_007184)
    Browse Sino Biological Human cDNA Clones
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for NISCH
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat NISCH

    Cell Line
    Products:
         
    GenScript Custom overexpressing Cell Line Services for NISCH
    Search LifeMap BioReagents cell lines for NISCH
    In Situ Assay
    Products:
       

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for NISCH


    (Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Oct 2013 via Entrez Gene).
    About This Section

    SuperPaths for NISCH About                                                                                                See pathways by source

    SuperPathContained pathways About
    1Cell adhesion Integrin-mediated cell adhesion and migration
    Cytoskeleton remodeling Fibronectin-binding integrins in cell motility0.32
    2Hypothetical Network for Drug Addiction
    Hypothetical Network for Drug Addiction

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways

    1 EMD Millipore Pathway for NISCH
        Cytoskeleton remodeling Fibronectin-binding integrins in cell motility


    1 GeneGo (Thomson Reuters) Pathway for NISCH
        Cytoskeleton remodeling Fibronectin-binding integrins in cell motility

    1 BioSystems Pathway for NISCH
        Hypothetical Network for Drug Addiction



    NISCH for pathways           About GeneDecksing

    Interactions:

        SABiosciences Gene Network CentralTM Interacting Genes and Proteins Network for NISCH

    STRING Interaction Network Preview (showing 5 interactants - click image to see 8)

    5/10 Interacting proteins for NISCH (Q9Y2I13 ENSP000003399584) via UniProtKB, MINT, STRING, and/or I2D (see all 10)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    ARP102753, ENSP000003638224I2D: score=1 STRING: ENSP00000363822
    IRS2Q9Y4H23, ENSP000003650164I2D: score=1 STRING: ENSP00000365016
    ITGA5P086483, ENSP000002933794I2D: score=3 STRING: ENSP00000293379
    IRS1P355683, ENSP000003048954I2D: score=1 STRING: ENSP00000304895
    IRS4O146543, ENSP000003612024I2D: score=2 STRING: ENSP00000361202
    About this table

    Gene Ontology (GO): 5/9 biological process terms (GO ID links to tree view) (see all 9):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006006glucose metabolic process IEA--
    GO:0006915apoptotic process IEA--
    GO:0007154cell communication ----
    GO:0008217regulation of blood pressure IEA--
    GO:0016601Rac protein signal transduction IEA--

    NISCH for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
    About This Section

    NISCH for compounds           About GeneDecksing

    Browse Small Molecules at EMD Millipore
    Enzo Life Sciences drugs & compounds for NISCH

    Browse Tocris compounds for NISCH

    2 DrugBank Compounds for NISCH    About this table
    CompoundSynonyms CAS #TypeActionsPubMed Ids
    Agmatine-- 306-60-5targetagonist7906055
    TizanidineTizanidina [INN-Spanish] (see all 3)51322-75-9targetagonist893017

    10/33 Novoseek inferred chemical compound relationships for NISCH gene (see all 33)    About this table
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    rilmenidine 96 31 7998582 (2), 1350732 (2), 1979731 (1), 9284427 (1) (see all 25)
    moxonidine 95.9 30 15028616 (2), 8068579 (1), 8872300 (1), 9284427 (1) (see all 27)
    idazoxan 90.2 10 10415909 (1), 10864097 (1), 8008210 (1), 1355439 (1) (see all 8)
    agmatine 88.4 24 17653850 (4), 9260995 (2), 10415899 (2), 7677340 (1) (see all 16)
    efaroxan 87.5 2 10580423 (1), 12189026 (1)
    clonidine 87 37 1979731 (1), 8096770 (1), 7677340 (1), 8642798 (1) (see all 31)
    benazoline 86.7 2 10825384 (1), 9208095 (1)
    guanabenz 80.7 5 14506596 (1), 7677390 (1), 12189026 (1)
    cirazoline 78.6 2 9116592 (1), 9116583 (1)
    methyldopa 76.5 3 9284427 (1), 7735264 (1)

    Search CenterWatch for drugs/clinical trials and news about NISCH

    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    Conferences by KenesGroup, exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    RNAi Products from EMD Millipore,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
    Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Sirion Biotech, Primers from OriGene, SABiosciences, and/or QIAGEN )
    About This Section

    REFSEQ mRNAs for NISCH gene (3 alternative transcripts): 
    NM_001276293.1  NM_001276294.1  NM_007184.3  

    Unigene Cluster for NISCH:

    Nischarin
    Hs.435290  [show with all ESTs]
    Unigene Representative Sequence: NM_007184
    14 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000479054(uc003deg.1) ENST00000488380(uc003dec.1) ENST00000420808
    ENST00000489895 ENST00000488157 ENST00000490425 ENST00000464280 ENST00000481211
    ENST00000474188 ENST00000485765 ENST00000460759 ENST00000488243 ENST00000467594
    ENST00000345716(uc003ded.4 uc003dee.4)

    miRNA
    Products:
         
    QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat NISCH
    8/11 QIAGEN miScript miRNA Assays for microRNAs that regulate NISCH (see all 11):
    hsa-miR-374b* hsa-miR-3673 hsa-miR-15a hsa-miR-503 hsa-miR-497 hsa-miR-646 hsa-miR-424 hsa-miR-16
    SwitchGear 3'UTR luciferase reporter plasmidNISCH 3' UTR sequence
    Inhib. RNA
    Products:
         
    Browse for Gene Knock-down Tools from EMD Millipore
    OriGene RNAi products in human, mouse, rat for NISCH
    QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat NISCH
    Clone
    Products:
         
    OriGene clones in human, mouse for NISCH (see all 7)
    OriGene ORF clones in mouse, rat for NISCH
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector: NISCH (NM_007184)
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for NISCH
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat NISCH
    Primer
    Products:
        
    OriGene qPCR primer pairs and template standards for NISCH
    OriGene qSTAR qPCR primer pairs in human, mouse for NISCH
    SABiosciences RT2 qPCR Primer Assay in human, mouse, rat NISCH
      QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat NISCH
      QIAGEN QuantiFast Probe-based Assays in human, mouse, rat NISCH

    Additional mRNA sequence: 

    AB023192.1 AF058290.1 AF082516.1 AK027331.1 AK027428.1 AK075000.1 AK097732.1 AK291398.1 
    AK297657.1 AK302441.1 AK315001.1 AL117432.1 AL137405.1 BC038102.2 BC050552.1 BC054494.1 
    BC056900.1 

    24/31 DOTS entries (see all 31):

    DT.100665695  DT.40305252  DT.100665694  DT.91963176  DT.100863171  DT.211714  DT.100665696  DT.100665690 
    DT.120854842  DT.95200862  DT.100665693  DT.92444758  DT.100846780  DT.100665697  DT.120854855  DT.95373494 
    DT.95373496  DT.95373517  DT.102834400  DT.95194538  DT.95373499  DT.95373505  DT.100044307  DT.100665689 

    24/512 AceView cDNA sequences (see all 512):

    BU557318 CR622669 BQ059505 AU279842 BF727193 AI888003 AA809374 BQ062280 
    BX431001 AA323023 AI926473 BU784317 AA812285 BQ063541 CK000716 BX952301 
    AI801671 AA493834 AA617988 CR623596 BQ063712 BQ066558 BG755139 CA841351 

    GeneLoc Exon Structure

    5/18 Alternative Splicing Database (ASD) splice patterns (SP) for NISCH (see all 18)    About this scheme

    ExUns: 1 ^ 2 ^ 3 ^ 4 ^ 5 ^ 6 ^ 7 ^ 8a · 8b ^ 9 ^ 10 ^ 11a · 11b · 11c ^ 12a · 12b ^ 13a · 13b ^ 14a · 14b · 14c ^ 15 ^ 16a · 16b · 16c · 16d ^
    SP1:                    -           -                             -                                               -                       -     -               
    SP2:                                                                                                              -                       -     -               
    SP3:                                                                                                                                                            
    SP4:                                                                                                              -                       -     -               
    SP5:                                                                                                                                                            

    ExUns: 17a · 17b · 17c ^ 18 ^ 19a · 19b · 19c · 19d ^ 20 ^ 21a · 21b · 21c ^ 22a · 22b ^ 23a · 23b ^ 24a · 24b · 24c ^ 25 ^ 26 ^ 27 ^ 28a · 28b · 28c · 28d ·
    SP1:                                                                                                                                                            
    SP2:  -     -     -     -     -     -                                                                                                                           
    SP3:                                                                                                                          -                 -     -     -   
    SP4:                                                                                                                                                            
    SP5:                                                                                                  -                       -                                 

    ExUns: 28e ^ 29
    SP1:            
    SP2:            
    SP3:  -         
    SP4:            
    SP5:            


    ECgene alternative splicing isoforms for NISCH

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    NISCH expression in normal human tissues (normalized intensities)      NISCH embryonic expression: see
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: TTGGGAGTGA
    NISCH Expression
    About this image


    NISCH expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database 
     5/2 selected tissues (see all 2) fully expand
     
     Brain (Nervous System)    fully expand to see all 13 entries
             Thalamus
             Olfactory Bulb   
     
     Skeletal Muscle (Muscoskeletal System)    fully expand to see all 2 entries
             Muscle Progenitor Cells Mandibular Arch Muscles

    See NISCH Protein Expression from SPIRE MOPED and PaxDB
    Genevestigator expression for NISCH

    SOURCE GeneReport for Unigene cluster: Hs.435290

    UniProtKB/Swiss-Prot: NISCH_HUMAN, Q9Y2I1
    Tissue specificity: Isoform 1, isoform 3 and isoform 4 are expressed in brain. Isoform 1 is expressed in endocrine
    tissues

        SABiosciences Custom PCR Arrays for NISCH
    Primer
    Products:
    OriGene qPCR primer pairs and template standards for NISCH
    OriGene qSTAR qPCR primer pairs in human, mouse for NISCH
    SABiosciences RT2 qPCR Primer Assay in human, mouse, rat NISCH
    QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat NISCH
    QIAGEN QuantiFast Probe-based Assays in human, mouse, rat NISCH
    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for NISCH

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    This gene was present in the common ancestor of animals.

    Orthologs for NISCH gene from 7/14 species (see all 14)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Nisch5 nischarin   --   14 (19.09 cM) 31170930 
    chicken
    (Gallus gallus)
    Aves NISCH1 nischarin 63.7(n)
    65.06(a)
      415893  XM_414245.3  XP_414245.3 
    lizard
    (Anolis carolinensis)
    Reptilia NISCH6
    Uncharacterized protein
    57(a)
    1 ↔ 1
    2(154564768-154601963)
    African clawed frog
    (Xenopus laevis)
    Amphibia AW159232.12   -- 72.43(n)    AW159232.1 
    zebrafish
    (Danio rerio)
    Actinopterygii nisch1 nischarin 57.49(n)
    52.72(a)
      553087  XM_002666230.2  XP_002666276.2 
    fruit fly
    (Drosophila melanogaster)
    Insecta CG118076
    --
    35(a)
    1 ↔ 1
    2R(11088801-11091971)
    worm
    (Caenorhabditis elegans)
    Secernentea F13E9.13
    CELE_F13E9.11
    SD22 like protein3
    Protein F13E9.11
    45(a)3
    46.32(n)1
    40.77(a)1
      IV(10871424-10873083)3
    1779811  NM_069615.41  NP_502016.21 


    ENSEMBL Gene Tree for NISCH (if available)
    TreeFam Gene Tree for NISCH (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section
    Paralogs for NISCH gene
    STK11IP2  

    NISCH for paralogs           About GeneDecksing



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
    About This Section

    10/956 SNPs in NISCH are shown (see all 956)    About this table     
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 3 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    VAR_0461304
    ----see VAR_0461302 V I mis40--------
    rs1162097401,2
    F--52419835(+) CTCTCA/GCACCC 3 -- us2k11Minor allele frequency- G:0.01NA 120
    rs1439156621,2
    --52419838(+) TCACAA/CCCTCC 3 -- us2k10--------
    rs1165878481,2
    F--52420020(+) GAGACA/GAATGC 3 -- us2k11Minor allele frequency- G:0.02WA 118
    rs20709141,2
    C,F,H--52420204(-) GGCCTA/GCGTGC 3 -- us2k110Minor allele frequency- G:0.07EA MN NS NA 2378
    rs583785541,2
    C,F--52420229(+) CACCAC/GTTCCC 3 -- us2k11Minor allele frequency- G:0.01EA 120
    rs1126578171,2
    C--52420315(+) TCCCTC/G/TACCGC 3 -- us2k11CSA 1
    rs2016159171,2
    --52420347(+) GCTGGC/TGGCTC 3 -- us2k10--------
    rs1121756991,2
    C,F--52420424(+) GGCCCG/AGCCCA 3 -- us2k11Minor allele frequency- A:0.50CSA 2
    rs10350021,2
    C,F,H--52420512(+) CCCCAT/CGTCCT 3 -- us2k114Minor allele frequency- C:0.02NA WA CSA EA 378

    HapMap Linkage Disequilibrium report for NISCH (52489134 - 52527088 bp)

    Structural Variations
         Database of Genomic Variants (DGV) 5 variations for NISCH:    About this table     
    Variant IDTypeSubtypePubMed ID
    nsv3819CNV Insertion18451855
    dgv5137n71CNV Loss21882294
    nsv834697CNV Loss17160897
    nsv876789CNV Loss21882294
    nsv876794CNV Loss21882294


    Human Gene Mutation Database (HGMD): NISCH
    SABiosciences Cancer Mutation PCR Assays
    SeqTarget long-range PCR primers for resequencing NISCH
    DNA2.0 Custom Variant and Variant Library Synthesis for NISCH

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Conferences by KenesGroup, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section
    18 diseases for NISCH:    About MalaCards
    rosacea    morphine dependence    congestive heart failure    brain disease
    essential hypertension    sclerosing cholangitis    hypertension    cholangitis
    ichthyosis    breast cancer    blindness    pheochromocytoma
    alzheimer's disease    diabetes mellitus    obesity    cerebritis
    neuronitis    pancreatitis

    2 diseases from the University of Copenhagen DISEASES database for NISCH:
    Morphine dependence     Sclerosing cholangitis

    NISCH for disorders           About GeneDecksing


    Congresses - knowledge worth sharing:  
    Alzheimer's & Parkinson's Diseases Congress (ADPD) 18 - 22 March 2015

    10/18 Novoseek inferred disease relationships for NISCH gene (see all 18)    About this table

    Disease   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    xerostomia 59.5 3 7496051 (1), 8807142 (1), 19041371 (1)
    hypertension arterial 45 1 2343243 (1)
    systemic hypertension 40.2 3 10874850 (1), 8852385 (1), 11728244 (1)
    withdrawal syndrome 36.1 1 8726097 (1)
    essential hypertension 31.4 1 9532644 (1)
    depressive state 27 2 8852385 (1), 11728244 (1)
    ventricular hypertrophy 23 1 9747912 (1)
    heart failure congestive 21.2 1 11728244 (1)
    major depression 13.1 3 9051774 (2), 10415944 (1)
    brain diseases 7.68 1 10415944 (1)

    Genetic Association Database (GAD): NISCH
    Human Genome Epidemiology (HuGE) Navigator: NISCH (4 documents)

    Export disorders for NISCH gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    PubMed articles for NISCH gene, integrated from 9 sources (see all 194):
    (articles sorted by number of sources associating them with NISCH)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Imidazoline receptor antisera-selected (IRAS) cDNA: cloning and characterization. (PubMed id 10882231)1, 2, 3, 9 Piletz J.E....Reis D.J. (2000)
    2. Insulin receptor substrate 4 associates with the protein IRAS. (PubMed id 11912194)1, 2, 3 Sano H....Lienhard G.E. (2002)
    3. IRAS splice variants. (PubMed id 15028621)1, 2, 9 Piletz J.E....Ziegler D. (2003)
    4. Human Nischarin/imidazoline receptor antisera-selected protein is targeted to the endosomes by a combined action of a PX domain and a coiled-coil region. (PubMed id 15475348)1, 2, 9 Lim K.-P. and Hong W. (2004)
    5. IRAS, the human homologue of Nischarin, prolongs survival of transfected PC12 cells. (PubMed id 12868002)1, 2, 9 Dontenwill M....Bousquet P. (2003)
    6. Meta-analysis identifies 13 new loci associated with waist-hip ratio and reveals sexual dimorphism in the genetic basis of fat distr ibution. (PubMed id 20935629)1, 4 Heid I.M....Lindgren C.M. (2010)
    7. Variation at the NFATC2 locus increases the risk of t hiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipr il and rosiglitazone Medication (DREAM) study. (PubMed id 20628086)1, 4 Bailey S.D....Anand S. (2010)
    8. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (2004)
    9. IRAS is an anti-apoptotic protein. (PubMed id 15028619)1, 2 Dontenwill M.... Bousquetd P. (2003)
    10. Prediction of the coding sequences of unidentified human genes. XIII. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. (PubMed id 10231032)1, 2 Nagase T.... Ohara O. (1999)

    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section
     ANDOR
    Aliases
    Disorders
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section
    Entrez Gene: 11188 HGNC: 18006 AceView: NISCH Ensembl:ENSG00000010322 euGenes: HUgn11188
    ECgene: NISCH H-InvDB: NISCH

    (According to HUGE)
    About This Section
    HUGE: KIAA0975

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
    Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
    About This Section
    NameDescription
    PharmGKB entry for NISCH Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section
    Patent Information for NISCH gene:
    Search GeneIP for patents involving NISCH

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, Gene Editing from DNA2.0 and Sirion Biotech, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Sirion Biotech, Cell lines from GenScript, and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or Enzo Life Sciences, In Situ Hybridization Assays from
    Advanced Cell Diagnostics, Animal models from inGenious Targeting Laboratory, genOway)
    About This Section

     
     EMD Millipore Custom Antibody & Bulk Services
     EMD Millipore Preclinical / Clinical Development Services
     EMD Millipore Immunoassay Services
     EMD Millipore Target Screening & Profiling Services

      
     Browse Antibodies   Browse Cell Culture Products  
     Browse ELISAs   Browse Flow Cytometry Kits  
     Browse Primer Pairs   Browse Kinase Activity Assays/Reagents  
     Browse ELISpot Kits/Development Modules   Browse TFB/Immunoprecipitation Assays  
     Browse Apoptosis Detection Kits/Reagents   Browse Ubiquitin Proteasome Pathway (UPP) Assay Kits/Reagents  
     Browse DNA Damage/Repair Kits/Reagents   Browse Multiplex/Array Assay Kits/Reagents  
     Browse Cell Selection/Detection Kits/Reagents   Browse Secondary Antibodies/Controls/Staining Reagents  
     Browse Protease Activity Assays and Reagents   Browse Recombinant/Natural Proteins  
     Browse Stem Cell Products   Browse Tocris Biochemicals & Compounds  
     Browse cDNA Clones   Browse Proteome Profiler Antibody Arrays  
     Browse OriGene Antibodies   OriGene RNAi products in human, mouse, rat for NISCH  
     OriGene qPCR primer pairs and template standards for NISCH   Browse OriGene Protein Over-expression Lysates  
     OriGene MassSpec something-or-other for NISCH   OriGene clones in human, mouse for NISCH  
     OriGene qSTAR qPCR primer pairs in human, mouse for NISCH   OriGene Purified Protein for NISCH  
     OriGene ORF clones in mouse, rat for NISCH   OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling  
     OriGene Custom Antibody Services for NISCH   OriGene Custom Protein Services for NISCH  

     
     
     QIAGEN Custom miScript Target Protector blocks miRNA-binding site of in human, mouse, rat NISCH
     QIAGEN SeqTarget long-range PCR primers for resequencing NISCH
     QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat NISCH
     QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat NISCH
     QIAGEN QuantiFast Probe-based Assays in human, mouse, rat NISCH
     QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat NISCH
     GenScript Custom Purified and Recombinant Proteins Services for NISCH GenScript cDNA clones with any tag delivered in your preferred vector for NISCH
     GenScript Custom Assay Services for NISCH GenScript Custom Superior Antibodies Services for NISCH
     GenScript Custom overexpressing Cell Line Services for NISCH CloneReady with Over 120,000 Genes
     Gene Synthesis: Any Gene in Any Vector Vector-based siRNA and miRNA, Ready for Transfection
     Gene Mutant Library, Variants up to 10^11 Plasmid Preparation
     Custom Peptide Services
     Search for Antibodies & Assays

     Regulatory tfbs in NISCH promoter
     Search Chromatin IP Primers for NISCH
     RT2 qPCR Primer Assay in human, mouse, rat NISCH
     GNC Network for NISCH
     SABiosciences Custom PCR Arrays for NISCH
     Search Tocris compounds for NISCH
     Browse Sino Biological Proteins and Antibodies
     Browse Sino Biological Cell Lysates
     Browse Sino Biological cDNA Clones
     4000+ Proteins
     Search Sino Biological for antibodies, proteins & pathways
     Protein Production Services
     Transfection Reagents
     Protein A/G/L resins
     Isotyping reagents
     Drugs for NISCH
     Search www.enzolifesciences.com for proteins, assays, substrates, inhibitors & antibodies
     Novus Tissue Slides
     NISCH antibodies
     NISCH proteins
     Antibodies for NISCH
     See all of Abcam's Antibodies, Kits and Proteins for NISCH
     Custom Antibody / Protein Production Service
     Bulk Purchasing
     Advantages of Rabbit Monoclonal antibodies
     Abcam protocols and scientific support
     Browse ProSpec Recombinant Proteins




     Proteins for NISCH
     Antibodies for NISCH
     ELISAs for NISCH
     CLIAs for NISCH
     Search LifeMap BioReagents cell lines for NISCH
     Gene Synthesis
     Protein Engineering
     Variant Library Synthesis
     Codon Optimization
     Protein Production and Purification
     Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for NISCH
     SwitchGear 3'UTR luciferase reporter plasmids for NISCH
     SwitchGear Promoter luciferase reporter plasmids for NISCH
     Search ThermoFisher Antibodies for NISCH
     Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat NISCH
     inGenious Targeting Laboratory - Custom generated mouse model solutions for NISCH
     inGenious Targeting Laboratory - Custom generated inducible mouse model solutions for NISCH
    Customized:
     lentivirus for stable overexpression
     lentivirus expression plasmids for stable overexpression
     adenovirus for overexpression of NISCH
     LSBio Antibodies in human, mouse, rat for NISCH
    Customized transgenic rodents for:
     Humanization
     Biomarker expression
     Off-target effect monitoring
     Translational medicine
     Tissue-specific gene expresssion
     Time-controlled gene expresssion
           
    GeneCards Homepage - Last full update: 23 Oct 2013 - Incrementals: 4 Nov 2013 , 7 Nov 2013 , 23 Jan 2014

    View Random Gene

    Category
    (GIFtS: 73)
    transforming growth factor, beta 1
    GIFtS Group
    The GeneCards human gene database gene index: 1 3 5 6 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 


    Developed at the Crown Human Genome Center, Department of Molecular Genetics, the Weizmann Institute of Science

    Hot genes      Disease genes      NISCH gene at Home site.
    hostname: 356980-web2.xennexinc.com index build: 106 solr: 1.4