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Category   Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21
GIFtS

NINJ2 Gene

protein-coding   GIFtS: 47
GCID: GC12M000673

ninjurin 2

 Explore 5 diseases affiliated with
NINJ2 via our new
 Human Malady Compendium 
Biological research products
for NINJ2
    

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, and/or 10fRNAdb)
About This Section

Aliases
Ninjurin 21 2
Nerve Injury-Induced Protein 22 3
Ninjurin-21

External Ids:    HGNC: 78251   Entrez Gene: 48152   Ensembl: ENSG000001718407   OMIM: 6072975   UniProtKB: Q9NZG73   

Export aliases for NINJ2 gene to outside databases

Previous GC identifers: GC12M000526 GC12M000543


(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for NINJ2:
The protein encoded by this gene belongs to the ninjurin (for nerve injury induced) family. It is a cell surface
adhesion protein that is upregulated in Schwann cells surrounding the distal segment of injured nerve, and promotes
neurite outgrowth, thus may have a role in nerve regeneration after nerve injury. (provided by RefSeq, Oct 2011)

UniProtKB/Swiss-Prot: NINJ2_HUMAN, Q9NZG7
Function: Homophilic cell adhesion molecule that promotes axonal growth. May play a role in nerve regeneration and in
the formation and function of other tissues




(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section
RefSeq DNA sequence:
NC_000012.11  NC_018923.1  NT_009759.16  
Regulatory elements:
   SABiosciences Regulatory transcription factor binding sites in the NINJ2 gene promoter:
         Bach2   Brachyury   FOXO3   GATA-1   C/EBPalpha   FOXO3b   HOXA5   FOXO4   FOXO3a   FOXO1   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidNINJ2 promoter sequence
   Search SABiosciences Chromatin IP Primers for NINJ2

Epigenetics:
QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat NINJ2


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 12p13   Ensembl cytogenetic band:  12p13.33   HGNC cytogenetic band: 12p13

NINJ2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
NINJ2 gene location

GeneLoc information about chromosome 12         GeneLoc Exon Structure

GeneLoc location for GC12M000673:  view genomic region     (about GC identifiers)

Start:
673,462 bp from pter      End:
772,945 bp from pter
Size:
99,484 bases      Orientation:
minus strand

(According to 1UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to 2PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: NINJ2_HUMAN, Q9NZG7 (See protein sequence)
Recommended Name: Ninjurin-2  
Size: 142 amino acids; 15680 Da
Subcellular location: Membrane; Multi-pass membrane protein
Sequence caution: Sequence=AAH57766.2; Type=Erroneous initiation; Note=Translation N-terminally shortened;

Explore the universe of human proteins at neXtProt for NINJ2: NX_Q9NZG7

NINJ2 Protein expression data from MOPED and PaxDb:    About this image 

Estimated protein expression log10 (pmol).

REFSEQ proteins: NP_057617.2  
ENSEMBL proteins: 
 ENSP00000307552   ENSP00000380435   ENSP00000438831   ENSP00000415158  

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Uscn Proteins for NINJ2

Gene Ontology (GO): 2 cellular component terms (GO ID links to tree view):    About this table

GO IDQualified GO termEvidencePubMed IDs
GO:0005887integral to plasma membrane TAS10627596
GO:0016021integral to membrane ----


NINJ2 for ontologies           About GeneDecksing



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(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

NINJ2 for domains           About GeneDecksing

1 InterPro domain/family:
 IPR007007 Ninjurin

Graphical View of Domain Structure for InterPro Entry Q9NZG7

ProtoNet protein and cluster: Q9NZG7

1 Blocks protein family: IPB007007 Ninjurin

UniProtKB/Swiss-Prot: NINJ2_HUMAN, Q9NZG7
Similarity: Belongs to the ninjurin family


(According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
About This Section

Function Summary:

     UniProtKB/Swiss-Prot: NINJ2_HUMAN, Q9NZG7
Function: Homophilic cell adhesion molecule that promotes axonal growth. May play a role in nerve regeneration and in
the formation and function of other tissues
Induction: By nerve injury; in Schwann cells

miRNA
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1 QIAGEN miScript miRNA Assays for microRNA that regulate NINJ2:
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In Situ Assay
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Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for NINJ2

3 GenomeRNAi human phenotypes for NINJ2:
 Decreased homologous recombina  Increased G1 DNA content  Increased gamma-H2AX phosphory 


(Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
About This Section



Interactions:

    Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for NINJ2

Gene Ontology (GO): 4 biological process terms (GO ID links to tree view):    About this table

GO IDQualified GO termEvidencePubMed IDs
GO:0007155cell adhesion ----
GO:0007158neuron cell-cell adhesion TAS10627596
GO:0007399nervous system development TAS10627596
GO:0042246tissue regeneration IEA--


NINJ2 for ontologies           About GeneDecksing



(Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
About This Section
Browse Small Molecules at EMD Millipore
Browse drugs & compounds from Enzo Life Sciences

Browse Tocris compounds for NINJ2
Search CenterWatch for drugs/clinical trials and news about NINJ2 

(Secondary structures according to fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from EMD Millipore,
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Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
About This Section

REFSEQ mRNAs for NINJ2 gene: 
NM_016533.4  

Unigene Cluster for NINJ2:

Ninjurin 2
Hs.656450  [show with all ESTs]
Unigene Representative Sequence: AK296012
5 Ensembl transcripts including schematic representations, and UCSC links where relevant:
ENST00000305108(uc001qil.3) ENST00000397265 ENST00000542920 ENST00000433832(uc010sdr.2 uc010sds.2)
ENST00000537416

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Inhib. RNA
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Additional cDNA sequence: 

AF205633.1 AK296012.1 AK298043.1 AK315995.1 BC057766.1 

3 DOTS entries:

DT.212267  DT.95364223  DT.91828925 

24/72 AceView cDNA sequences (see all 72):

R06257 AW016477 AI393003 BU660855 CA446244 AA450074 AA743532 BM354140 
NM_016533 AA769168 AI333512 CA431480 W38567 CB243438 AI218723 BC057766 
BU953992 BM127826 BI598565 BU624562 BI603068 CB995632 AW016351 BG706186 

GeneLoc Exon Structure


(RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section

NINJ2 expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: TCTGTCTAAG

Microarray
RNAseq (Illumina Body Map)
(100×FPKM)½
SAGE (Serial Analysis of Gene Expression)

About this image

NINJ2 expression in embryonic tissues and stem cells
Expression by the Database of Embryonic development, Stem cell research, and Regenerative medicine    About this table
1 LifeMap In Vivo Development Anatomical Compartment/Cell 
Tissue Anatomical Compartment CellCategory (developmental path)
Umbilical CordUmbilical VeinsUmbilical Cord
Expression: Positive    Negative     Selective marker
Experimental details: Curated     Microarrays     In-situ hybridization
Stem Cell Differentiation: 4 LifeMap Cells 
NameCategory
HyStem+TGF?3+GDF5-induced SK11 cells (HyStem+TGF?3+GDF5 in...)Bone, Cartilage
HyStem+TGF?3+GDF5-induced SM30 cells (HyStem+TGF?3+GDF5 in...)Bone, Cartilage
HyStem+TGF?3+GDF5-induced 7PEND24 cells (HyStem+TGF?3+GDF5 in...)Bone
HyStem+TGF?3+GDF5-induced E15 cells (HyStem+TGF?3+GDF5 in...)Bone, Cartilage

See NINJ2 Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for NINJ2

SOURCE GeneReport for Unigene cluster: Hs.656450

UniProtKB/Swiss-Prot: NINJ2_HUMAN, Q9NZG7
Tissue specificity: Widely expressed. In adult, higher expression in the bone marrow and peripheral blood lymphocytes,
medium in the lung, lymph node, thyroid, uterus, thymus, spleen, prostate and skeletal muscle, lower in the liver,
placenta, brain, heart and kidney. In embryo, higher expression in the thymus, heart and liver, lower in the spleen,
lung, brain and kidney

    SABiosciences Custom PCR Arrays for NINJ2
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In Situ
Assay Products:
 

 
Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for NINJ2

(Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
About This Section

This gene was present in the common ancestor of animals.

Orthologs for NINJ2 gene from 6/15 species (see all 15)    About this table
Organism Taxonomic
classification
Gene Description Human
Similarity
Orthology
Type
Details
mouse
(Mus musculus)
Mammalia Ninj21 , 5 ninjurin 21, 5 77.93(n)1
73.24(a)1
  6 (56.90 cM)5
298621  NM_016718.21  NP_057927.11 
 1200933505 
chicken
(Gallus gallus)
Aves NINJ21 ninjurin 2 68.11(n)
65.47(a)
  418151  XM_416382.3  XP_416382.2 
lizard
(Anolis carolinensis)
Reptilia NINJ26
--
56(a)
1 ↔ 1
5(12084631-12091608)
African clawed frog
(Xenopus laevis)
Amphibia CD360436.12   -- 75.52(n)    CD360436.1 
zebrafish
(Danio rerio)
Actinopterygii LOC7992671 ninjurin-1-like 67.51(n)
63.03(a)
  799267  XM_001339607.2  XP_001339643.2 
fruit fly
(Drosophila melanogaster)
Insecta CG143946
CG116376
(see all 3)
--
34(a)
19(a)
(see all 3)
many ↔ many
many ↔ many
(see all 3)
3R(8520868-8523265)
3L(18885483-18886378)


ENSEMBL Gene Tree for NINJ2 (if available)
TreeFam Gene Tree for NINJ2 (if available) 

(Paralogs according to 1HomoloGene,
2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
About This Section
Paralogs for NINJ2 gene
NINJ12  
1 SIMAP similar gene for NINJ2 using alignment to 4 protein entries:     NINJ2_HUMAN (see all proteins):
NINJ1

NINJ2 for paralogs           About GeneDecksing



(SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section

10/2135 NCBI SNPs in NINJ2 are shown (see all 2135    About this table
Genomic DataTranscription Related DataAllele Frequencies
SNP IDValidClinical
significance
Chr 12 posSequence#AA
Chg
TypeMore#Allele
freq
PopTotal
sample
More
----------
rs735981121,2
C,--592095(+) TCCCCA/TGGAGA 1 -- us2k12Minor allele frequency- T:0.11WA NA 122
rs576715841,2
C,--592786(+) GCACAG/AACCCA 1 -- us2k12Minor allele frequency- A:0.02CSA WA 120
rs735981161,2
C,--593213(+) CCTGGG/AGTATG 1 -- int11Minor allele frequency- A:0.50WA 2
rs79562161,2
A--594060(+) CATGGA/GGGACC 1 -- int10--------
rs793322311,2
C,F,--594080(+) GAAGTC/TTGAAC 1 -- int11Minor allele frequency- T:0.19WA 118
rs79563261,2
A--594112(+) AGATTC/GAGTAA 1 -- int10--------
rs79563341,2
A--594142(+) TTAAAC/GTCCTA 1 -- spa10--------
rs735981171,2
C,F,--594239(+) CTTTGC/TTTGTC 1 -- int12Minor allele frequency- T:0.50WA CSA 4
rs79768301,2
A--594394(+) TGCACC/TCTGGA 1 -- int10--------
rs587233721,2
F,--594886(+) CAAAAG/AGTTAG 1 -- int11Minor allele frequency- A:0.21WA 118

HapMap Linkage Disequilibrium report for NINJ2 (673462 - 772945 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV): 9 variations for NINJ2
     3 CNVs: 53493 9677 3871
     6 Indels: 25042 45062 86134 25039 71423 75893

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(in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
About This Section

NINJ2 for disorders           About GeneDecksing

OMIM gene information: 607297    OMIM disorders: --

5 diseases for NINJ2:    About MalaCards
acrocallosal syndrome    alzheimer's disease    thyroiditis    prostatitis
neuronitis

1 disease from the University of Copenhagen DISEASES database for NINJ2:
Acrocallosal syndrome
Human Genome Epidemiology (HuGE) Navigator: NINJ2 (4 documents)

Export disorders for NINJ2 gene to outside databases

(in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
About This Section

PubMed articles for NINJ2 gene, integrated from 9 sources (see all 13):
(articles sorted by number of sources associating them with NINJ2)
    Utopia: connect your pdf to the dynamic
world of online information

  1. Ninjurin2, a novel homophilic adhesion molecule, is expressed in mature sensory and enteric neurons and promotes neurite outgrowth. (PubMed id 10627596)1, 2, 3 Araki T. and Milbrandt J. (2000)
  2. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
  3. The rs11833579 and rs12425791 polymorphisms and risk o f ischemic stroke in an Asian population: a meta-analysis. (PubMed id 22795341)1 Lian G....Li S. (2012)
  4. Association between 12p13 SNPs rs11833579/rs12425791 n ear NINJ2 gene and ischemic stroke in East Asian population: evidence from a met a-analysis. (PubMed id 22297388)1 Li B.H....Li J.C. (2012)
  5. Genetic polymorphisms of a novel vascular susceptibil ity gene, Ninjurin2 (NINJ2), are associated with a decreased risk of Alzheimer' s disease. (PubMed id 21674003)1 Lin K.P....Chen Y.C. (2011)
  6. No evidence for association of 12p13 SNPs rs11833579 and rs12425791 within NINJ2 gene with ischemic stroke in Chinese Han population . (PubMed id 21376321)1 Ding H....Wang D.W. (2011)
  7. NINJ2 polymorphism is associated with ischemic stroke in Chinese Han population. (PubMed id 21722921)1 Wan X.H....Wang X.F. (2011)
  8. Failure to validate association between 12p13 variant s and ischemic stroke. (PubMed id 20410525)1 (2010)
  9. [Strong association between the NINJ2 gene polymorphi sm and the susceptibility of stroke in Chinese Han population in Fangshan distr ict]. (PubMed id 20957003)1 Chen K....Qiu C.C. (2010)
  10. Genomewide association studies of stroke. (PubMed id 19369658)1 Ikram M.A....Wolf P.A. (2009)

(in PubMed, OMIM, and NCBI Bookshelf)
About This Section
 ANDOR
Aliases
Free Text  

  Query String
PubMed
OMIM
NCBI Bookshelf
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(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
About This Section
Entrez Gene: 4815 HGNC: 7825 AceView: NINJ2 Ensembl:ENSG00000171840 euGenes: HUgn4815
ECgene: NINJ2 H-InvDB: NINJ2

(According to HUGE)
About This Section
  --

(According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section
NameDescription
PharmGKB entry for NINJ2 Pharmacogenomics, SNPs, Pathways

(Patent information from GeneIP,
Licensable technologies from WIS Yeda, Salk, Tufts,
IP news from LifeMap Sciences, Inc.)
About This Section
Patent Information for NINJ2 gene:
Search GeneIP for patents involving NINJ2

GeneCards and IP:
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(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or Enzo Life Sciences),
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 Regulatory tfbs in NINJ2 promoter
 Search Chromatin IP Primers for NINJ2
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 Search for Antibodies for NINJ2 at Abcam
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 NINJ2 Proteins, Antibodies, CLIAs, and ELISAs
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 Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat NINJ2
       
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