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Category Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21

GIFtS
-

NFKB2 Gene

protein-coding GIFtS: 67
GCID: GC10P104144

Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer...

Microbiology & Infectious Diseases Congress

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at

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(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc, ⁷Ensembl, ⁸DME, ⁹miRBase, ¹⁰fRNAdb, ¹²H-InvDB, ¹³NCBI, ¹⁴NONCODE, and/or ¹⁵RNAdb)
About This Section

Aliases
Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 2 (P49/P100)¹ ²		NF-kB2²
LYT10² ³ ⁵		Nuclear Factor NF-Kappa-B P100 Subunit²
Lymphocyte Translocation Chromosome 10 Protein² ³		Nuclear Factor Of Kappa Light Chain Gene Enhancer In B-Cells 2²
Oncogene Lyt-10² ³		p105²
H2TF1² ³		p52²
DNA-Binding Factor KBF2² ³		Lyt10³
LYT-10²		Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells 2³

External Ids:	HGNC: 7795¹	Entrez Gene: 4791²	Ensembl: ENSG00000077150⁷	OMIM: 164012⁵	UniProtKB: Q00653³

Export aliases for NFKB2 gene to outside databases

Previous GC identifers: GC10P103047 GC10P103387 GC10P104285 GC10P103818 GC10P097787

(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for NFKB2 Gene:

This gene encodes one of the subunits of the transcription factor complex nuclear factor-kappa-B (NFkB). The NFkB

transcription factor complex is expressed in numerous cell types and functions as a central activator of genes

involved in inflammation and immune function. The NFkB complex can consist of different subunits that form both

homo- or heterodimers which bind specific kappa-B elements in target genes. This gene encodes the p100 subunit

that is processed into the active p52 subunit. This protein can function as both a transcriptional activator and

repressor, depending on its dimer partner. Alternate splicing results in both coding and non-coding variants.

(provided by RefSeq, May 2012)

GeneCards Summary for NFKB2 Gene:

NFKB2 (nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100)) is a protein-coding gene. Diseases associated with NFKB2 include splenic marginal zone lymphoma, and hepatopulmonary syndrome, and among its related super-pathways are Signal transduction AKT signaling and Mucin expression in CF via IL-6, IL-17 signaling pathways. GO annotations related to this gene include transcription coactivator activity and sequence-specific DNA binding transcription factor activity. An important paralog of this gene is NFKB1.

UniProtKB/Swiss-Prot: NFKB2_HUMAN, Q00653

Function: NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of

a series of signal transduction events that are initiated by a vast array of stimuli related to many biological

processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B

is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105,

NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the

individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable

affinity and specificity. Different dimer combinations act as transcriptional activators or repressors,

respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular

compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are

held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family.

In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to

different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to

the nucleus. In a non-canonical activation pathway, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates

NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B

RelB-p52 complexes. The NF-kappa-B heterodimeric RelB-p52 complex is a transcriptional activator. The NF-kappa-B

p52-p52 homodimer is a transcriptional repressor. NFKB2 appears to have dual functions such as cytoplasmic

retention of attached NF-kappa-B proteins by p100 and generation of p52 by a cotranslational processing. The

proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function.

p52 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in

immune response and acute phase reactions. p52 and p100 are respectively the minor and major form; the processing

of p100 being relatively poor. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the

HIV enhancer in synergy with p65

summary for NFKB2 Gene:

NF-kappaB (nuclear factor-kappa B) is a rapidly acting primary transcription factor found in all cell types.

It is involved in cellular responses to stimuli such as cytokines and stress and plays a key role in

regulating the immune response to infection. In unstimulated cells NF-kappaB dimers are sequestered

inactively in the cytoplasm by a protein complex called inhibitor of kappa B (IkappaB). IkappaB inactivates

NF-kappaB by masking the nuclear localization signals (NLS). Activation of NF-kappaB occurs via degradation

of IkappaB, a process that is initiated by its phosphorylation by IkappaB kinase (IKK). Phosphorylated IvB

becomes dissociated from NF-kappaB, unmasking the NLS. Phosphorylation also results in IkappaB

ubiquitination and targeting to the proteasome. NF-kappaB can now enter the nucleus and regulate gene

expression. NF-kappaB turns on expression of IkappaB forming a negative feedback loop.

Gene Wiki entry for NFKB2 Gene

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 73), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section

RefSeq DNA sequence:

NC_000010.10 NT_030059.13 NC_018921.2

Regulatory elements:

SABiosciences Regulatory transcription factor binding sites in the NFKB2 gene promoter:
p53 STAT3
Other transcription factors

SwitchGear Promoter luciferase reporter plasmids (see all 3): NFKB2 promoter sequence

Search SABiosciences Chromatin IP Primers for NFKB2

Epigenetics:

QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat NFKB2

Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 10q24 Ensembl cytogenetic band: 10q24.32 HGNC cytogenetic band: 10q24

NFKB2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
NFKB2 gene location

GeneLoc information about chromosome 10 GeneLoc Exon Structure

GeneLoc location for GC10P104144: view genomic region (about GC identifiers)

Start:	104,153,867 bp from pter	End:	104,162,281 bp from pter
Size:	8,415 bases	Orientation:	plus strand

(According to UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE ¹MOPED, ²PaxDb, and ³MAXQB RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Cloud-Clone Corp.,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Cloud-Clone Corp., Ontologies according to Gene Ontology Consortium 01 Oct 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, and/or Cloud-Clone Corp.)
About This Section

UniProtKB/Swiss-Prot: NFKB2_HUMAN, Q00653 (See protein sequence)

Recommended Name: Nuclear factor NF-kappa-B p100 subunit

Size: 900 amino acids; 96749 Da

Subunit: Component of the NF-kappa-B RelB-p52 complex. Homodimer; component of the NF-kappa-B p52-p52 complex.

Component of the NF-kappa-B p65-p52 complex. Component of the NF-kappa-B p52-c-Rel complex. NFKB2/p52 interacts

with NFKBIE. Component of a complex consisting of the NF-kappa-B p50-p50 homodimer and BCL3. Directly interacts

with MEN1

Subcellular location: Nucleus. Cytoplasm. Note=Nuclear, but also found in the cytoplasm in an inactive form

complexed to an inhibitor (I-kappa-B)

Sequence caution: Sequence=CAA43715.1; Type=Frameshift; Positions=456, 458, 470, 900;

3 PDB 3D structures from and Proteopedia for NFKB2:

1A3Q (3D)

2D96 (3D)

3DO7 (3D)

Secondary accessions: A8K9D9 D3DR83 Q04860 Q9BU75 Q9H471 Q9H472

Alternative splicing: 3 isoforms: Q00653-1 Q00653-3 Q00653-4 (Ref.1 (CAA43716.1) sequence(s) differ(s) from that shown due to frameshifts in positions 407, 414)

Explore the universe of human proteins at neXtProt for NFKB2: NX_Q00653

Explore proteomics data for NFKB2 at MOPED

Post-translational modifications:

UniProtKB: While translation occurs, the particular unfolded structure after the GRR repeat promotes the generation of p52

making it an acceptable substrate for the proteasome. This process is known as cotranslational processing. The

processed form is active and the unprocessed form acts as an inhibitor (I kappa B-like), being able to form

cytosolic complexes with NF-kappa B, trapping it in the cytoplasm. Complete folding of the region downstream of

the GRR repeat precludes processing

UniProtKB: Subsequent to MAP3K14-dependent serine phosphorylation, p100 polyubiquitination occurs then triggering its

proteasome-dependent processing

UniProtKB: Constitutive processing is tightly suppressed by its C-terminal processing inhibitory domain, named PID, which

contains the death domain

View modification sites using PhosphoSitePlus

View neXtProt modification sites for NX_Q00653

NFKB2 Protein expression data from MOPED¹, PaxDb² and MAXQB³ : About this image

REFSEQ proteins (3 alternative transcripts):

NP_001070962.1 NP_001248332.1 NP_002493.3

ENSEMBL proteins:

ENSP00000470826 ENSP00000358983 ENSP00000471586 ENSP00000189444 ENSP00000410256

Reactome Protein details: Q00653
Human Recombinant Protein Products for NFKB2:

	EMD Millipore Purified and/or Recombinant NFKB2 Protein
	Browse R&D Systems for human recombinant proteins
	Browse recombinant and purified proteins available from Enzo Life Sciences
	OriGene Purified Proteins for NFKB2 OriGene Protein Over-expression Lysate for NFKB2 OriGene MassSpec for NFKB2 OriGene Custom Protein Services for NFKB2
	GenScript Custom Purified and Recombinant Proteins Services for NFKB2
	Novus Biologicals NFKB2 Protein
	Browse Sino Biological Recombinant Proteins Browse Sino Biological Cell Lysates
	Browse ProSpec Recombinant Proteins
	Browse Proteins at Cloud-Clone Corp.

Gene Ontology (GO): 5/6 cellular component terms (GO ID links to tree view) (see all 6): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005634	nucleus	IDA	--
GO:0005654	nucleoplasm	TAS	--
GO:0005730	NOT nucleolus	IDA	--
GO:0005737	cytoplasm	IDA	--
GO:0005829	cytosol	TAS	--

NFKB2 for ontologies About GeneDecksing

NFKB2 Antibody Products:

	EMD Millipore Mono- and Polyclonal Antibodies for the study of NFKB2
	R&D Systems Antibodies for NFKB2
	Cell Signaling Technology (CST) Antibodies for NFKB2 (NFkB-p100)
	OriGene Antibodies for NFKB2 OriGene Custom Antibody Services for NFKB2
	GenScript Custom Superior Antibodies Services for NFKB2
	Novus Biologicals NFKB2 Antibodies
	Abcam antibodies for NFKB2
	Browse Antibodies at Cloud-Clone Corp.
	ThermoFisher Antibodies for NFKB2
	LSBio Antibodies in human, mouse, rat for NFKB2

Assay Products for NFKB2:

	EMD Millipore Kits and Assays for the Analysis of NFKB2
	OriGene Custom Assay Services for NFKB2
	R&D Systems Transcription Factor Binding & Immunoprecipitation Assays for NFKB2
	GenScript Custom Assay Services for NFKB2
	Browse Enzo Life Sciences for kits & assays
	Browse ELISAs at Cloud-Clone Corp. Browse CLIAs at Cloud-Clone Corp.

(According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

HGNC Gene Families:

ANKRD: Ankyrin repeat domain containing

5/10 InterPro protein domains (see all 10):

IPR014756 Ig_E-set

IPR008967 p53-like_TF_DNA-bd

IPR011029 DEATH-like_dom

IPR002909 IPT_TIG_rcpt

IPR013783 Ig-like_fold

Graphical View of Domain Structure for InterPro Entry Q00653

ProtoNet protein and cluster: Q00653

5 Blocks protein domains:

IPB000451 NF-kappa-B/Rel/dorsal family

IPB000488 Death domain

IPB002110 Ankyrin repeat signature

IPB002909 Cell surface receptor IPT/TIG

IPB011539 Rel homology

UniProtKB/Swiss-Prot: NFKB2_HUMAN, Q00653

Domain: The C-terminus of p100 might be involved in cytoplasmic retention, inhibition of DNA-binding by p52

homodimers, and/or transcription activation (By similarity)

Domain: The glycine-rich region (GRR) appears to be a critical element in the generation of p52

Similarity: Contains 7 ANK repeats

Similarity: Contains 1 death domain

Similarity: Contains 1 RHD (Rel-like) domain

NFKB2 for domains About GeneDecksing

(According to ¹UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or ²DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
bound targets from SABiosciences, miRNA Gene Targets from miRTarBase, shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Sirion Biotech, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, Vector BioLabs, and Sirion Biotech, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Oct 2013 via Entrez Gene.)
About This Section

Molecular Function:

UniProtKB/Swiss-Prot Summary: NFKB2_HUMAN, Q00653