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Aliases for NFE4 Gene

Aliases for NFE4 Gene

  • Nuclear Factor, Erythroid 4 2 3 5
  • Fetal Globin Activator NF-E4 3
  • Gamma-Globin Gene Activator 3
  • NF-E4 3

External Ids for NFE4 Gene

Previous GeneCards Identifiers for NFE4 Gene

  • GC07P102614

Summaries for NFE4 Gene

Entrez Gene Summary for NFE4 Gene

  • The erythroid-specific protein encoded by this gene, and the ubiquitous transcription factor CP2, form the stage selector protein (SSP) complex, which is involved in preferential expression of the gamma-globin genes in fetal erythroid cells. Alternate use of an in-frame upstream non-AUG (CUG) translation initiation codon, and a downstream AUG codon, results in two isoforms. While the long isoform (22 kDa) acts as an activator, the short isoform (14 kDa) has been shown to repress gamma-globin gene expression. This gene is located in an intron of the FBXL13 gene on the opposite strand. [provided by RefSeq, Jul 2008]

GeneCards Summary for NFE4 Gene

NFE4 (Nuclear Factor, Erythroid 4) is a Protein Coding gene.

UniProtKB/Swiss-Prot for NFE4 Gene

  • Functions as part of the SSP (stage selector protein) complex, a complex that contributes to the preferential expression of the gamma-gene in fetal erythroid cells by facilitating the interaction of the gamma-globin genes with enhancer elements contained in the locus control region (LCR). The complex binds to the stage selector element (SSE) in the proximal gamma-globin promoter. In contrast, isoform 2 acts as a repressor of gamma-globin gene expression by preventing NFE2 and RNA polymerase II recruitment to the promoter.

No data available for Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for NFE4 Gene

Genomics for NFE4 Gene

Regulatory Elements for NFE4 Gene

Promoters for NFE4 Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters

ENSRs around NFE4 on UCSC Golden Path with GeneCards custom track

Genomic Location for NFE4 Gene

Chromosome:
7
Start:
102,973,437 bp from pter
End:
102,988,856 bp from pter
Size:
15,420 bases
Orientation:
Plus strand

Genomic View for NFE4 Gene

Genes around NFE4 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
NFE4 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for NFE4 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for NFE4 Gene

Proteins for NFE4 Gene

  • Protein details for NFE4 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q86UQ8-NFE4_HUMAN
    Recommended name:
    Transcription factor NF-E4
    Protein Accession:
    Q86UQ8

    Protein attributes for NFE4 Gene

    Size:
    179 amino acids
    Molecular mass:
    19019 Da
    Quaternary structure:
    • Component of the SSP (stage selector protein) complex, which appears to be a heteromer of TFCP2 and 2 copies of NFE4. Interacts with HDAC1 and PCAF. Isoform 2 interacts with TFCP2.
    SequenceCaution:
    • Sequence=AAP13531.1; Type=Miscellaneous discrepancy; Note=Unusual initiator. The initiator methionine is coded by a non-canonical CTG leucine codon.; Evidence={ECO:0000305};

    Alternative splice isoforms for NFE4 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for NFE4 Gene

Proteomics data for NFE4 Gene at MOPED

Post-translational modifications for NFE4 Gene

  • Acetylation at Lys-43 prolongs the protein half-life by preventing ubiquitin-mediated degradation and reduces the interaction between NF-E4 and HDAC1, potentially maximizing the activating ability of the factor at the gamma-promoter.
  • Ubiquitinated; leading to its degradation by the proteasome. Acetylation at Lys-43 prevents ubiquitination.
  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

Other Protein References for NFE4 Gene

REFSEQ proteins:

No data available for DME Specific Peptides for NFE4 Gene

Domains & Families for NFE4 Gene

Protein Domains for NFE4 Gene

ProtoNet:

Suggested Antigen Peptide Sequences for NFE4 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

genes like me logo Genes that share domains with NFE4: view

No data available for Gene Families and UniProtKB/Swiss-Prot for NFE4 Gene

Function for NFE4 Gene

Molecular function for NFE4 Gene

UniProtKB/Swiss-Prot Function:
Functions as part of the SSP (stage selector protein) complex, a complex that contributes to the preferential expression of the gamma-gene in fetal erythroid cells by facilitating the interaction of the gamma-globin genes with enhancer elements contained in the locus control region (LCR). The complex binds to the stage selector element (SSE) in the proximal gamma-globin promoter. In contrast, isoform 2 acts as a repressor of gamma-globin gene expression by preventing NFE2 and RNA polymerase II recruitment to the promoter.

Phenotypes for NFE4 Gene

GenomeRNAi human phenotypes for NFE4:
genes like me logo Genes that share phenotypes with NFE4: view

Animal Model Products

No data available for Enzyme Numbers (IUBMB) , Gene Ontology (GO) - Molecular Function , Human Phenotype Ontology , Animal Models , miRNA , Transcription Factor Targets and HOMER Transcription for NFE4 Gene

Localization for NFE4 Gene

Subcellular locations from UniProtKB/Swiss-Prot for NFE4 Gene

No data available for Subcellular locations from COMPARTMENTS and Gene Ontology (GO) - Cellular Components for NFE4 Gene

Pathways & Interactions for NFE4 Gene

SuperPathways for NFE4 Gene

No Data Available

Interacting Proteins for NFE4 Gene

Selected Interacting proteins: Q86UQ8-NFE4_HUMAN for NFE4 Gene via I2D

Gene Ontology (GO) - Biological Process for NFE4 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006351 transcription, DNA-templated IEA --
genes like me logo Genes that share ontologies with NFE4: view

No data available for Pathways by source and SIGNOR curated interactions for NFE4 Gene

Drugs & Compounds for NFE4 Gene

No Compound Related Data Available

Transcripts for NFE4 Gene

mRNA/cDNA for NFE4 Gene

(1) REFSEQ mRNAs :
(1) Additional mRNA sequences :
(1) Ensembl transcripts including schematic representations, and UCSC links where relevant :

Unigene Clusters for NFE4 Gene

Transcription factor NF-E4:
Representative Sequences:

Alternative Splicing Database (ASD) splice patterns (SP) for NFE4 Gene

No ASD Table

Relevant External Links for NFE4 Gene

GeneLoc Exon Structure for
NFE4
ECgene alternative splicing isoforms for
NFE4

Expression for NFE4 Gene

mRNA expression in normal human tissues for NFE4 Gene

mRNA differential expression in normal tissues according to GTEx for NFE4 Gene

This gene is overexpressed in Whole Blood (x36.2) and Adrenal Gland (x6.7).

Protein differential expression in normal tissues from HIPED for NFE4 Gene

This gene is overexpressed in CD8 Tcells (69.0).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB and MOPED for NFE4 Gene



SOURCE GeneReport for Unigene cluster for NFE4 Gene Hs.621233

mRNA Expression by UniProt/SwissProt for NFE4 Gene

Q86UQ8-NFE4_HUMAN
Tissue specificity: Specifically expressed in fetal liver, cord blood and bone marrow. Also expressed in the K562 and HEL cell lines, which constitutively express the fetal globin genes.
genes like me logo Genes that share expression patterns with NFE4: view

Protein tissue co-expression partners for NFE4 Gene

Primer Products

In Situ Assay Products

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery for NFE4 Gene

Orthologs for NFE4 Gene

Evolution for NFE4 Gene

ENSEMBL:
Gene Tree for NFE4 (if available)
TreeFam:
Gene Tree for NFE4 (if available)

No data available for Orthologs for NFE4 Gene

Paralogs for NFE4 Gene

No data available for Paralogs for NFE4 Gene

Variants for NFE4 Gene

Sequence variations from dbSNP and Humsavar for NFE4 Gene

SNP ID Clin Chr 07 pos Sequence Context AA Info Type
VAR_046622 -
VAR_046623 -

Relevant External Links for NFE4 Gene

HapMap Linkage Disequilibrium report
NFE4

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot , Structural Variations from Database of Genomic Variants (DGV) and Variation tolerance for NFE4 Gene

Disorders for NFE4 Gene

No disorders were found for NFE4 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot , Genatlas and External Links for NFE4 Gene

Publications for NFE4 Gene

  1. Induction of human fetal globin gene expression by a novel erythroid factor, NF-E4. (PMID: 11003662) Zhou W. … Jane S.M. (Mol. Cell. Biol. 2000) 2 3 4 23 67
  2. Repression of human gamma-globin gene expression by a short isoform of the NF-E4 protein is associated with loss of NF-E2 and RNA polymerase II recruitment to the promoter. (PMID: 16263792) Zhao Q. … Jane S.M. (Blood 2006) 3 23
  3. Site-specific acetylation of the fetal globin activator NF-E4 prevents its ubiquitination and regulates its interaction with the histone deacetylase, HDAC1. (PMID: 15273251) Zhao Q. … Jane S.M. (J. Biol. Chem. 2004) 3 23
  4. The role of WDR5 in silencing human fetal globin gene expression. (PMID: 22689669) Xu Z. … Zhao Q. (Haematologica 2012) 3
  5. PRMT5-mediated methylation of histone H4R3 recruits DNMT3A, coupling histone and DNA methylation in gene silencing. (PMID: 19234465) Zhao Q. … Jane S.M. (Nat. Struct. Mol. Biol. 2009) 3

Products for NFE4 Gene

Sources for NFE4 Gene

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