Aliases
for NFE4 gene
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc,
7Ensembl,
8DME,
9miRBase,
and/or 10fRNAdb)
About This Section
|
Subcategory (RNA class): lncRNA
Quality score for this RNA gene is 2
| Aliases |
|---|
| NF-E42 | | Fetal Globin Activator NF-E42 | | Gamma-Globin Gene Activator2 | | Transcription Factor NF-E42 |
| External Ids: | Entrez Gene: 581602 | Ensembl: ENSG000002302577 | OMIM: 6121335 | UniProtKB: Q86UQ83 | | ORGUL members: | |
|---|
| NONCODE:n341286 | |
Export aliases for NFE4 gene to outside databases
|---|
Summaries
for NFE4 gene(According to Entrez Gene,
Tocris Bioscience,
Wikipedia's
Gene Wiki,
PharmGKB,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
|
Entrez Gene summary for NFE4:
The erythroid-specific protein encoded by this gene, and the ubiquitous transcription factor CP2, form the stageselector protein (SSP) complex, which is involved in preferential expression of the gamma-globin genes in fetalerythroid cells. Alternate use of an in-frame upstream non-AUG (CUG) translation initiation codon, and a downstreamAUG codon, results in two isoforms. While the long isoform (22 kDa) acts as an activator, the short isoform (14 kDa)has been shown to repress gamma-globin gene expression. This gene is located in an intron of the FBXL13 gene on theopposite strand. (provided by RefSeq, Jul 2008)
UniProtKB/Swiss-Prot: NFE4_HUMAN, Q86UQ8Function: Functions as part of the SSP (stage selector protein) complex, a complex that contributes to the preferentialexpression of the gamma-gene in fetal erythroid cells by facilitating the interaction of the gamma-globin genes withenhancer elements contained in the locus control region (LCR). The complex binds to the stage selector element (SSE)in the proximal gamma-globin promoter. In contrast, isoform 2 acts as a repressor of gamma-globin gene expression bypreventing NFE2 and RNA polymerase II recruitment to the promoter
|
Genomic Views
for NFE4 gene
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to
UCSC (hg19) and
Ensembl (release 69),
Regulatory elements and Epigenetics data according to
QIAGEN,
SABiosciences, and/or
SwitchGear Genomics)
About This Section
| RefSeq DNA sequence:- NC_000007.13 NC_018918.1 NT_007933.15 NT_079596.2
Regulatory elements:
 Search SABiosciences Regulatory transcription factor binding sites for NFE4
Other transcription factors
Search SABiosciences Chromatin IP Primers for NFE4
Epigenetics:
| | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat NFE4 |
Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track
Entrez Gene cytogenetic band: 7q22.1 Ensembl cytogenetic band: 7q22.1 NFE4 Gene in genomic location: bands according to Ensembl, locations according to
 (and/or Entrez Gene and/or Ensembl if different)
GeneLoc information about chromosome 7 GeneLoc Exon Structure GeneLoc location for GC07P102614: view genomic region
(about GC identifiers)
Start:
|
102,613,884 bp from pter |
End:
|
102,629,303 bp from pter |
Size:
|
15,420 bases |
Orientation:
|
plus strand |
1 alternative location:
| Chr7+,CRA_TCAG 101,974,250-101,979,480 |
|
Proteins
for NFE4 gene
(According to
1UniProtKB,
HORDE,
neXtProt,
Ensembl,
and/or Reactome,
Modification sites according to 2PhosphoSitePlus,
Specific Peptides from DME,
Protein expression images according to data from SPIRE MOPED and PaxDb,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from
EMD Millipore,
R&D Systems,
GenScript,
Enzo Life Sciences,
OriGene,
Novus Biologicals,
Sino Biological,
ProSpec, and/or
Uscn,
Biochemical Assays by
EMD Millipore,
R&D Systems,
OriGene,
GenScript,
Cell Signaling Technology,
Enzo Life Sciences, and/or
Uscn,
Ontologies according to Gene
Ontology Consortium 01 Mar 2013 and
Entrez Gene,
Antibodies by
EMD Millipore,
R&D Systems,
GenScript,
Cell Signaling Technology,
OriGene,
Novus Biologicals,
Thermo Fisher Scientific,
Abcam, and/or
Uscn)
About This Section
|
UniProtKB/Swiss-Prot: NFE4_HUMAN, Q86UQ8 (See
protein sequence)Recommended Name: Transcription factor NF-E4 Size: 179 amino acids; 19019 Da
Subunit: Component of the SSP (stage selector protein) complex, which appears to be a heteromer of TFCP2 and 2 copiesof NFE4. Interacts with HDAC1 and PCAF. Isoform 2 interacts with TFCP2
Subcellular location: Nucleus (Probable)
Sequence caution: Sequence=AAP13531.1; Type=Miscellaneous discrepancy; Note=Unusual initiator. The initiator methionineis coded by a non-canonical CTG leucine codon;Alternative initiation: 2 isoforms: Q86UQ8-1 Q86UQ8-2 Explore the universe of human proteins at neXtProt for NFE4: NX_Q86UQ8
Post-translational modifications:
Acetylation at Lys-43 prolongs the protein half-life by preventing ubiquitin-mediated degradation and reduces theinteraction between NF-E4 and HDAC1, potentially maximizing the activating ability of the factor at the gamma-promoter1
Ubiquitinated; leading to its degradation by the proteasome. Acetylation at Lys-43 prevents ubiquitination1
View modification sites using PhosphoSitePlus2View neXtProt modification sites for NX_Q86UQ8
NFE4 Protein expression data from MOPED and PaxDb: --
REFSEQ proteins: NP_001078855.1
Human Recombinant Protein Products:
Gene Ontology (GO): 1 cellular component term (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0005634 | nucleus |
IEA | -- |
NFE4 for ontologies About GeneDecksing
NFE4 Antibody Products:
Assay Products for NFE4: |
Protein
Domains /
Families
for NFE4 gene(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
| ProtoNet protein and cluster: Q86UQ8 |
Function
for NFE4 gene
(According to 1UniProtKB,
Genatlas,
LifeMap Discovery™,
IUBMB, and/or
2DME,
Human phenotypes from GenomeRNAi,
Animal models from MGI Mar 06 2013,
bound targets from SABiosciences,
miRNA Gene Targets from miRTarBase
shRNA from
OriGene,
RNAi from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
microRNA from QIAGEN,
Gene Editing from DNA2.0,
Clones from EMD Millipore,
OriGene,
SwitchGear Genomics,
GenScript,
Sino Biological,
DNA2.0,
and Vector BioLabs,
Cell Lines from GenScript,
LifeMap BioReagents,
In Situ Hybridization Assays from Advanced Cell Diagnostics,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene.)
About This Section
|
Function Summary:
UniProtKB/Swiss-Prot: NFE4_HUMAN, Q86UQ8Function: Functions as part of the SSP (stage selector protein) complex, a complex that contributes to the preferentialexpression of the gamma-gene in fetal erythroid cells by facilitating the interaction of the gamma-globin genes withenhancer elements contained in the locus control region (LCR). The complex binds to the stage selector element (SSE)in the proximal gamma-globin promoter. In contrast, isoform 2 acts as a repressor of gamma-globin gene expression bypreventing NFE2 and RNA polymerase II recruitment to the promoter
Clone
Products: |  | Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore | |  | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for NFE4
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for NFE4
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling 
| |  | GenScript: all cDNA clones in your preferred vector: NFE4 (NM_001085386) | |  | Browse Sino Biological Human cDNA Clones | |  | DNA2.0 Custom Codon Optimized Gene
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In Situ Assay
Products: |
 | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for NFE4 |
|
Pathways & Interactions
for NFE4 gene
(Pathways according to
EMD Millipore,
R&D Systems,
Cell Signaling Technology,
KEGG,
PharmGKB,
BioSystems,
Reactome,
Tocris Bioscience,
GeneGo (Thomson Reuters),
QIAGEN,
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Interaction Networks according to
SABiosciences,
and/or STRING,
Interactions according to 1UniProtKB,
2MINT,
3I2D, and/or
4STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene).
About This Section
|
Interactions:
Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for NFE4
5 Interacting proteins for NFE4 (Q86UQ83) via UniProtKB, MINT, STRING, and/or I2DAbout this table
Gene Ontology (GO): 2 biological process terms (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0006351 | transcription, DNA-dependent |
IEA | -- | | GO:0006355 | regulation of transcription, DNA-dependent |
IEA | -- |
NFE4 for ontologies About GeneDecksing
|
Drugs & Compounds
for NFE4 gene(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
EMD Millipore, Tocris Bioscience
HMDB,
BitterDB, and/or
Novoseek, and Drugs according to
DrugBank,
Enzo Life Sciences, and/or
PharmGKB, with drugs/clinical trials/news
search links to CenterWatch)
About This Section
|
 Browse Tocris compounds for NFE4
Search CenterWatch for drugs/clinical trials and news about NFE4
|
Transcripts
for NFE4 gene(Secondary structures according to
fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
transcript ids from Ensembl
with links to UCSC,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
shRNA from
OriGene,
microRNA from QIAGEN,
Tagged/untagged cDNA clones from
OriGene,
SwitchGear Genomics,
GenScript,
DNA2.0,
Vector BioLabs,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN
)
About This Section
|
REFSEQ mRNAs for NFE4 gene: NM_001085386.1 Unigene Cluster for NFE4: Transcription factor NF-E4 Hs.621233 Unigene Representative Sequence: BC0369381 Ensembl transcript including schematic representation, and UCSC links where relevant: ENST00000420058(lincRNA)
Clone
Products: | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for NFE4
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for NFE4
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling
| | | GenScript: all cDNA clones in your preferred vector: NFE4 (NM_001085386) | | | DNA2.0 Custom Codon Optimized Gene
Synthesis Service for NFE4 | | | Search Vector BioLabs for ready-to-use adenovirus/AAV for human, mouse, rat NFE4 |
Additional cDNA sequence: BC036938.1 1 DOTS entry: DT.99929296
GeneLoc Exon Structure
|
Expression
for NFE4 gene
(RNA expression data according to
H-InvDB,
NONCODE,
miRBase, and
RNAdb,
Expression images according to data from
BioGPS,
Illumina Human BodyMap, and
CGAP
SAGE,
Sets of similar genes according to GeneDecks,
in vivo and in vitro expression data from LifeMap Discovery™,
plus additional links to
Genevestigator, and/or
SOURCE, and/or
BioGPS, and/or
UniProtKB,
PCR Arrays from
SABiosciences,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN,
In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section
| Expression evidence for NFE4:none
NFE4 expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: --
About this image
Genevestigator expression for NFE4
SOURCE GeneReport for Unigene cluster: Hs.621233
UniProtKB/Swiss-Prot: NFE4_HUMAN, Q86UQ8Tissue specificity: Specifically expressed in fetal liver, cord blood and bone marrow. Also expressed in the K562 andHEL cell lines, which constitutively express the fetal globin genes
SABiosciences Custom PCR Arrays for NFE4
Primer
Products: | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for NFE4
Browse OriGene validated miRNA SYBR primer pairs
| | | Search SABiosciences RT2 qPCR
Primer Assays in human, mouse, rat NFE4 | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat NFE4 | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat NFE4 | In Situ
Assay Products: |
| Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for NFE4 |
Orthologs
for NFE4 gene
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
,
5MGI Mar 06 2013,
with possible further links to
Flybase
and/or
WormBase,
and/or
6Ensembl pan taxonomic compara ,
Gene Trees according to Ensembl and
TreeFam)
About This Section
|
--
ENSEMBL Gene Tree for NFE4 (if available)
TreeFam Gene Tree for NFE4 (if available) |
Paralogs
for NFE4 gene(Paralogs according to
1HomoloGene,
2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
About This Section
| -- |
Genomic Variants
for NFE4 gene(SNPs/Variants according to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE,
UniProtKB, and
DNA2.0,
Linkage Disequilibrium by HapMap,
Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene
Mutation Database (HGMD) and the Locus Specific Mutation
Databases (LSDB), Blood group antigen gene mutations by BGMUT,
Resequencing Primers from QIAGEN,
Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section
|
| Genomic Data | Transcription Related Data | Allele Frequencies | | SNP ID | Valid | Clinical
significance | Chr 7 pos | Sequence | # | AA
Chg | Type | More | # | Allele
freq | Pop | Total
sample | More |
|---|
HapMap Linkage Disequilibrium report for NFE4 (102613884 - 102629303 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV) variations for NFE4: -- | SABiosciences Cancer Mutation PCR Assays |
|
Disorders
/ Diseases
for NFE4 gene
(in which this Gene is Involved, According to MalaCards,
OMIM, UniProtKB,
the University of Copenhagen DISEASES
database,
Genatlas, GeneTests,
GAD,
HuGE Navigator,
and/or TGDB.)
About This Section
|
 NFE4 for disorders About MalaCards
NFE4 for disorders About GeneDecksing
OMIM gene information: 612133
OMIM disorders: --
Export disorders for NFE4 gene to outside databases
|
Publications
for NFE4 gene (in
PubMed.
Associations of this gene to articles via
1Entrez Gene,
2UniProtKB/Swiss-Prot,
3HGNC,
4GAD,
5PharmGKB,
6HMDB,
7DrugBank,
8UniProtKB/TrEMBL,
9 Novoseek, and/or
10fRNAdb)
About This Section
|
PubMed articles for NFE4 gene integrated from 9 sources:
(articles sorted by number of sources associating them with NFE4) | |  | Utopia: connect your pdf to the dynamic
world of online information |
- Induction of human fetal globin gene expression by a novel erythroid factor, NF-E4. (PubMed id 11003662)1, 2, 9 Zhou W....Jane S.M. (2000)
- Repression of human gamma-globin gene expression by a short isoform of the NF-E4 protein is associated with loss of NF-E2 and RNA polymerase II recruitment to the promoter. (PubMed id 16263792)1, 2, 9 Zhao Q.... Jane S.M. (2006)
- Site-specific acetylation of the fetal globin activator NF-E4 prevents its ubiquitination and regulates its interaction with the histone deacetylase, HDAC1. (PubMed id 15273251)1, 2, 9 Zhao Q.... Jane S.M. (2004)
- The role of p22 NF-E4 in human globin gene switching. (PubMed id 15084587)1, 2 Zhou W.... Jane S.M. (2004)
- PRMT5-mediated methylation of histone H4R3 recruits DNMT3A, coupling histone and DNA methylation in gene silencing. (PubMed id 19234465)1 Zhao Q....Jane S.M. (2009)
- Biochemical characterization of the developmental sta ge- and tissue-specific erythroid transcription factor, NF-E4. (PubMed id 8144507)9 Yang Z. and Engel J.D. (1994)
- Developmental regulation of the human beta-globin cluster. (PubMed id 8770365)9 Jane S.M....Cunningham J.M. (1995)
|
External Searches for NFE4 gene
(in PubMed,
OMIM, and NCBI Bookshelf)
About This Section
|
|
Genome Databases showing NFE4 gene
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
Kegg,
and/or
H-InvDB)
About This Section
|
|
Other Databases showing NFE4 gene
(According to HUGE)
About This Section
| -- |
Specialized Databases showing NFE4 gene(According to PharmGKB,
ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section
|
|
About This Section
| Patent Information for NFE4 gene:
Search GeneIP for patents involving NFE4
GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search
|
Products
for NFE4 gene(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or
Enzo Life Sciences),
In Situ Hybridization Assays from
Advanced Cell Diagnostics
About This Section
|
 | |
 | |
 |
| | |  | Browse OriGene Antibodies | | OriGene shRNA RFP for NFE4 | | | OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for NFE4 | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for NFE4 | | | OriGene Protein Over-expression Lysate for NFE4 | | Browse OriGene Fluorogenic Cell Assay Kits | | | OriGene siRNA for NFE4 | | Browse 3'-UTR reporter clones for miRNA target validation | | | OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for NFE4 | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for NFE4 | | | Browse OriGene GFP tagged cDNA clones in CMV expression vector | | Browse OriGene MicroRNA Expression Plasmids | | | Browse OriGene basic RS shRNAs | | Browse OriGene validated miRNA SYBR primer pairs | | | Browse OriGene full length recombinant human proteins expressed in human HEK293 cells | | OriGene custom cloning services - gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling | | | OriGene Custom Antibody Services for NFE4 | | OriGene Custom Protein Services for NFE4 | | | OriGene Custom Immunoassay Development | | |
| |
 |
 |
| | | QIAGEN Custom miScript Target Protector blocks miRNA-binding site of in human, mouse, rat NFE4 | | | Search QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing NFE4 | | | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat NFE4 | | | QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat NFE4 | | | QIAGEN QuantiFast Probe-based Assays in human, mouse, rat NFE4 | | | QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat NFE4 |
| |
 |  |  |  | |
| | | | Search Tocris compounds for NFE4 |
| |  |  |  |  | | | | |
 |
 |
 |
 | | | | | | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for NFE4 |
|  |  |  | | | | Search ThermoFisher Antibodies for NFE4 |
| | | Search Vector BioLabs for ready-to-use adenovirus/AAV for human, mouse, rat NFE4 |
|
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