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Aliases for NFE2 Gene

Aliases for NFE2 Gene

  • Nuclear Factor, Erythroid 2 2 3 5
  • Nuclear Factor, Erythroid-Derived 2 45 KDa Subunit 3 4
  • Nuclear Factor (Erythroid-Derived 2), 45kDa 2 3
  • Nuclear Factor (Erythroid-Derived 2), 45kD 2 3
  • Leucine Zipper Protein NF-E2 3 4
  • P45 NF-E2 3 4
  • NF-E2 3
  • P45 3

External Ids for NFE2 Gene

Previous GeneCards Identifiers for NFE2 Gene

  • GC12M054813
  • GC12M054402
  • GC12M052972
  • GC12M054685
  • GC12M051725

Summaries for NFE2 Gene

GeneCards Summary for NFE2 Gene

NFE2 (Nuclear Factor, Erythroid 2) is a Protein Coding gene. Diseases associated with NFE2 include Polycythemia and Myelofibrosis. Among its related pathways are Platelet activation, signaling and aggregation and Factors involved in megakaryocyte development and platelet production. GO annotations related to this gene include transcription factor activity, sequence-specific DNA binding and transcription coactivator activity. An important paralog of this gene is NFE2L2.

UniProtKB/Swiss-Prot for NFE2 Gene

  • Component of the NF-E2 complex essential for regulating erythroid and megakaryocytic maturation and differentiation. Binds to the hypersensitive site 2 (HS2) of the beta-globin control region (LCR). This subunit (NFE2) recognizes the TCAT/C sequence of the AP-1-like core palindrome present in a number of erythroid and megakaryocytic gene promoters. Requires MAFK or other small MAF proteins for binding to the NF-E2 motif. May play a role in all aspects of hemoglobin production from globin and heme synthesis to procurement of iron.

Gene Wiki entry for NFE2 Gene

No data available for Entrez Gene Summary , Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for NFE2 Gene

Genomics for NFE2 Gene

Regulatory Elements for NFE2 Gene

Enhancers for NFE2 Gene
GeneHancer Identifier Score Enhancer Sources TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor Binding Sites within enhancer Other Gene Targets for Enhancer

Enhancers around NFE2 on UCSC Golden Path with GeneCards custom track

Promoters for NFE2 Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters

ENSRs around NFE2 on UCSC Golden Path with GeneCards custom track

Transcription factor binding sites by QIAGEN in the NFE2 gene promoter:

Genomic Location for NFE2 Gene

Chromosome:
12
Start:
54,292,107 bp from pter
End:
54,301,121 bp from pter
Size:
9,015 bases
Orientation:
Minus strand

Genomic View for NFE2 Gene

Genes around NFE2 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
NFE2 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for NFE2 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for NFE2 Gene

Proteins for NFE2 Gene

  • Protein details for NFE2 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q16621-NFE2_HUMAN
    Recommended name:
    Transcription factor NF-E2 45 kDa subunit
    Protein Accession:
    Q16621
    Secondary Accessions:
    • Q07720
    • Q6ICV9

    Protein attributes for NFE2 Gene

    Size:
    373 amino acids
    Molecular mass:
    41473 Da
    Quaternary structure:
    • Homodimer; can bind DNA as a homodimer. Erythroid transcription activator nuclear factor erythroid-derived 2 (NF-E2), composed of a heterodimer of NFE2 and MAFK, possesses transactivation activity on beta-globin. Also forms high affinity heterodimer with MAFG; the interaction promotes erythropoiesis. Interacts (via the PXY motif 1) with ITCH (via the WW 1 domain); the interaction promotes Lys63-linked ubiquitination of NFE2, translocates it to the cytoplasm and inhibits its transactivation activity. Interacts with KMT2D/MLL2; the interaction promotes transactivation of the beta-globin locus (By similarity). Interacts with MAPK8 (phosphorylated form); the interaction leads to phosphorylation of NFE2 in undifferentiated cells (By similarity).

    Three dimensional structures from OCA and Proteopedia for NFE2 Gene

neXtProt entry for NFE2 Gene

Proteomics data for NFE2 Gene at MOPED

Post-translational modifications for NFE2 Gene

  • Phosphorylated on serine residues. In undifferentiated erythrocytes, phosphorylated by MAPK8 which then leads to ubiquitination and protein degradation.
  • Sumoylated. Sumoylation is required for translocation to nuclear bodies PODs, anchoring to the gene loci, and transactivation of the beta-globin gene.
  • Ubiquitinated mainly by Lys63-linked ubiquitin. Polyubiquitination with Lys63-linked ubiquitin by ITCH retains NFE2 in the cytoplasm preventing its transactivation activity. In undifferentiated erythrocyte, ubiquitinated after MAPK8-mediatd phosphorylation leading to protein degradation (By similarity).
  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

Other Protein References for NFE2 Gene

No data available for DME Specific Peptides for NFE2 Gene

Domains & Families for NFE2 Gene

Gene Families for NFE2 Gene

Protein Domains for NFE2 Gene

Suggested Antigen Peptide Sequences for NFE2 Gene

Graphical View of Domain Structure for InterPro Entry

Q16621

UniProtKB/Swiss-Prot:

NFE2_HUMAN :
  • The PXY motifs are required for binding WW domains. PXY1 is required to promote transactivation of beta-globin and for hyperacetylation of histone H3, but not for binding to the HS2 promoter site (By similarity).
  • Belongs to the bZIP family. CNC subfamily.
Domain:
  • The PXY motifs are required for binding WW domains. PXY1 is required to promote transactivation of beta-globin and for hyperacetylation of histone H3, but not for binding to the HS2 promoter site (By similarity).
  • Contains 1 bZIP (basic-leucine zipper) domain.
Family:
  • Belongs to the bZIP family. CNC subfamily.
genes like me logo Genes that share domains with NFE2: view

Function for NFE2 Gene

Molecular function for NFE2 Gene

GENATLAS Biochemistry:
nuclear factor,erythroid derived NF-E2 Capncollar (CNC) family of transcription factor,dimerizing with TFCP2 to form the stage selector protein complex,binding to LCR of globin loci
UniProtKB/Swiss-Prot Function:
Component of the NF-E2 complex essential for regulating erythroid and megakaryocytic maturation and differentiation. Binds to the hypersensitive site 2 (HS2) of the beta-globin control region (LCR). This subunit (NFE2) recognizes the TCAT/C sequence of the AP-1-like core palindrome present in a number of erythroid and megakaryocytic gene promoters. Requires MAFK or other small MAF proteins for binding to the NF-E2 motif. May play a role in all aspects of hemoglobin production from globin and heme synthesis to procurement of iron.

Gene Ontology (GO) - Molecular Function for NFE2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000976 transcription regulatory region sequence-specific DNA binding IBA --
GO:0047485 protein N-terminus binding IPI 11863372
genes like me logo Genes that share ontologies with NFE2: view
genes like me logo Genes that share phenotypes with NFE2: view

Animal Models for NFE2 Gene

MGI Knock Outs for NFE2:

Animal Model Products

Transcription Factor Targets for NFE2 Gene

Selected GeneGlobe predicted Target genes for NFE2
Targeted motifs for NFE2 Gene
HOMER Transcription Factor Regulatory Elements motif NFE2
  • Consensus sequence: GATGACTCAGCA Submotif: canonical Cell Type: K562 GEO ID: GSE31477

No data available for Enzyme Numbers (IUBMB) , Human Phenotype Ontology and miRNA for NFE2 Gene

Localization for NFE2 Gene

Subcellular locations from UniProtKB/Swiss-Prot for NFE2 Gene

Nucleus, PML body. Cytoplasm. Note=The sumoylated form locates to the nuclear bodies PML oncogenic domains (PODs). Translocated to the cytoplasm through interaction with ITCH.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for NFE2 Gene COMPARTMENTS Subcellular localization image for NFE2 gene
Compartment Confidence
cytoskeleton 5
nucleus 5
cytosol 2
mitochondrion 1

Gene Ontology (GO) - Cellular Components for NFE2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005737 cytoplasm IBA --
GO:0015629 actin cytoskeleton IDA --
GO:0016605 PML body IEA --
genes like me logo Genes that share ontologies with NFE2: view

Pathways & Interactions for NFE2 Gene

genes like me logo Genes that share pathways with NFE2: view

Pathways by source for NFE2 Gene

Gene Ontology (GO) - Biological Process for NFE2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006337 nucleosome disassembly TAS 9305852
GO:0008015 blood circulation TAS 8355703
GO:0045893 positive regulation of transcription, DNA-templated TAS 9305852
genes like me logo Genes that share ontologies with NFE2: view

No data available for SIGNOR curated interactions for NFE2 Gene

Drugs & Compounds for NFE2 Gene

(5) Drugs for NFE2 Gene - From: HMDB and Novoseek

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
heme Pharma Agonist 0

(9) Additional Compounds for NFE2 Gene - From: Novoseek and HMDB

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
Fe2+
  • Armco iron
  • Carbonyl iron
  • FE
  • Ferrovac e
  • Hematite
15438-31-0
genes like me logo Genes that share compounds with NFE2: view

Transcripts for NFE2 Gene

Unigene Clusters for NFE2 Gene

Nuclear factor (erythroid-derived 2), 45kDa:
Representative Sequences:

Alternative Splicing Database (ASD) splice patterns (SP) for NFE2 Gene

ExUns: 1a · 1b ^ 2 ^ 3 ^ 4a · 4b ^ 5a · 5b · 5c
SP1: - -
SP2: - - -
SP3: -
SP4:

Relevant External Links for NFE2 Gene

GeneLoc Exon Structure for
NFE2
ECgene alternative splicing isoforms for
NFE2

Expression for NFE2 Gene

mRNA expression in normal human tissues for NFE2 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for NFE2 Gene

This gene is overexpressed in Whole Blood (x48.7).

Protein differential expression in normal tissues from HIPED for NFE2 Gene

This gene is overexpressed in Cervix (65.7).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MOPED, and MaxQB for NFE2 Gene



SOURCE GeneReport for Unigene cluster for NFE2 Gene Hs.75643

mRNA Expression by UniProt/SwissProt for NFE2 Gene

Q16621-NFE2_HUMAN
Tissue specificity: Expressed in hematopoietic cells and also in colon and testis.
genes like me logo Genes that share expression patterns with NFE2: view

Protein tissue co-expression partners for NFE2 Gene

- Elite partner

Primer Products

Orthologs for NFE2 Gene

This gene was present in the common ancestor of animals.

Orthologs for NFE2 Gene

Organism Taxonomy Gene Similarity Type Details
cow
(Bos Taurus)
Mammalia NFE2 35
  • 89.28 (n)
  • 91.15 (a)
NFE2 36
  • 91 (a)
OneToOne
dog
(Canis familiaris)
Mammalia NFE2 35
  • 88.38 (n)
  • 92.23 (a)
NFE2 36
  • 92 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Nfe2 35
  • 84.99 (n)
  • 88.74 (a)
Nfe2 16
Nfe2 36
  • 89 (a)
OneToOne
chimpanzee
(Pan troglodytes)
Mammalia NFE2 35
  • 99.46 (n)
  • 99.73 (a)
NFE2 36
  • 100 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Nfe2 35
  • 85.7 (n)
  • 90.08 (a)
platypus
(Ornithorhynchus anatinus)
Mammalia -- 36
  • 37 (a)
ManyToMany
lizard
(Anolis carolinensis)
Reptilia NFE2 36
  • 54 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia nfe2 35
  • 50.71 (n)
  • 45.93 (a)
zebrafish
(Danio rerio)
Actinopterygii nfe2 36
  • 32 (a)
OneToOne
fruit fly
(Drosophila melanogaster)
Insecta cnc 37
  • 49 (a)
cnc 36
  • 8 (a)
OneToMany
sea squirt
(Ciona savignyi)
Ascidiacea -- 36
  • 11 (a)
OneToMany
Species with no ortholog for NFE2:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • chicken (Gallus gallus)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • oppossum (Monodelphis domestica)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for NFE2 Gene

ENSEMBL:
Gene Tree for NFE2 (if available)
TreeFam:
Gene Tree for NFE2 (if available)

Paralogs for NFE2 Gene

Paralogs for NFE2 Gene

(1) SIMAP similar genes for NFE2 Gene using alignment to 4 proteins:

genes like me logo Genes that share paralogs with NFE2: view

Variants for NFE2 Gene

Sequence variations from dbSNP and Humsavar for NFE2 Gene

SNP ID Clin Chr 12 pos Sequence Context AA Info Type
rs7296759 -- 54,302,536(+) GCCTG(C/T)TTCTG upstream-variant-2KB
rs12814631 -- 54,297,315(+) gtgcc(A/C)ccgca intron-variant, upstream-variant-2KB
rs7304860 -- 54,293,705(+) TTAGC(C/T)GGGCA intron-variant
rs7309809 -- 54,298,505(+) tccac(A/T)tcaaa intron-variant, upstream-variant-2KB
rs7314607 -- 54,299,724(+) CAGGG(C/G)TCTTA intron-variant, upstream-variant-2KB

Structural Variations from Database of Genomic Variants (DGV) for NFE2 Gene

Variant ID Type Subtype PubMed ID
nsv832420 CNV Loss 17160897

Variation tolerance for NFE2 Gene

Residual Variation Intolerance Score: 18.5% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 0.57; 12.23% of all genes are more intolerant (likely to be disease-causing)

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot and Relevant External Links for NFE2 Gene

Disorders for NFE2 Gene

MalaCards: The human disease database

(3) MalaCards diseases for NFE2 Gene - From: DISEASES, Novoseek, and GeneCards

Disorder Aliases PubMed IDs
polycythemia
  • erythrocythemia
myelofibrosis
  • agnogenic myeloid metaplasia
essential thrombocythemia
  • hemorrhagic thrombocythemia
- elite association - COSMIC cancer census association via MalaCards
Search NFE2 in MalaCards View complete list of genes associated with diseases

Relevant External Links for NFE2

Genetic Association Database (GAD)
NFE2
Atlas of Genetics and Cytogenetics in Oncology and Haematology:
NFE2
genes like me logo Genes that share disorders with NFE2: view

No data available for UniProtKB/Swiss-Prot and Genatlas for NFE2 Gene

Publications for NFE2 Gene

  1. Purification of the human NF-E2 complex: cDNA cloning of the hematopoietic cell-specific subunit and evidence for an associated partner. (PMID: 8355703) Ney P.A. … Neinhuis A.W. (Mol. Cell. Biol. 1993) 2 3 4 23 67
  2. NF-E2 domination over Nrf2 promotes ROS accumulation and megakaryocytic maturation. (PMID: 19901266) Motohashi H. … Yamamoto M. (Blood 2010) 3 23
  3. USF and NF-E2 cooperate to regulate the recruitment and activity of RNA polymerase II in the beta-globin gene locus. (PMID: 20236933) Zhou Z. … Bungert J. (J. Biol. Chem. 2010) 3 23
  4. A critical role for the transcription factor Scl in platelet production during stress thrombopoiesis. (PMID: 16763211) McCormack M.P. … Curtis D.J. (Blood 2006) 3 23
  5. Sumoylation of p45/NF-E2: nuclear positioning and transcriptional activation of the mammalian beta-like globin gene locus. (PMID: 16287851) Shyu Y.-C. … Shen C.-K.J. (Mol. Cell. Biol. 2005) 3 23

Products for NFE2 Gene

Sources for NFE2 Gene

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