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Aliases for NET1 Gene

Aliases for NET1 Gene

  • Neuroepithelial Cell Transforming 1 2 3 5
  • ARHGEF8 3 4
  • Guanine Nucleotide Regulatory Protein (Oncogene) 3
  • Rho Guanine Nucleotide Exchange Factor (GEF) 8 3
  • Rho Guanine Nucleotide Exchange Factor 8 4
  • Neuroepithelioma Transforming Gene 1 3
  • Small GTP-Binding Protein Regulator 3
  • P65 Net1 Proto-Oncogene Protein 3
  • Proto-Oncogene P65 Net1 4
  • NET1A 3

External Ids for NET1 Gene

Previous GeneCards Identifiers for NET1 Gene

  • GC10M005513
  • GC10P005428
  • GC10P005554
  • GC10P005408

Summaries for NET1 Gene

Entrez Gene Summary for NET1 Gene

  • This gene is part of the family of Rho guanine nucleotide exchange factors. Members of this family activate Rho proteins by catalyzing the exchange of GDP for GTP. The protein encoded by this gene interacts with RhoA within the cell nucleus and may play a role in repairing DNA damage after ionizing radiation. Pseudogenes of this gene are located on the long arms of chromosomes 1, 7 and 18. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Jul 2012]

GeneCards Summary for NET1 Gene

NET1 (Neuroepithelial Cell Transforming 1) is a Protein Coding gene. Diseases associated with NET1 include Orthostatic Intolerance and Breast Cancer. Among its related pathways are ERK Signaling and p75 NTR receptor-mediated signalling. GO annotations related to this gene include guanyl-nucleotide exchange factor activity and GTP-Rho binding. An important paralog of this gene is ARHGEF3.

UniProtKB/Swiss-Prot for NET1 Gene

  • Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase. May be involved in activation of the SAPK/JNK pathway Stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell death.

Gene Wiki entry for NET1 Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for NET1 Gene

Genomics for NET1 Gene

Regulatory Elements for NET1 Gene

Enhancers for NET1 Gene
GeneHancer Identifier Score Enhancer Sources TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor Binding Sites within enhancer Other Gene Targets for Enhancer

Enhancers around NET1 on UCSC Golden Path with GeneCards custom track

Promoters for NET1 Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters

ENSRs around NET1 on UCSC Golden Path with GeneCards custom track

Genomic Location for NET1 Gene

5,412,551 bp from pter
5,459,056 bp from pter
46,506 bases
Plus strand

Genomic View for NET1 Gene

Genes around NET1 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
NET1 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for NET1 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for NET1 Gene

Proteins for NET1 Gene

  • Protein details for NET1 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Recommended name:
    Neuroepithelial cell-transforming gene 1 protein
    Protein Accession:
    Secondary Accessions:
    • Q12773
    • Q96D82
    • Q99903
    • Q9UEN6

    Protein attributes for NET1 Gene

    596 amino acids
    Molecular mass:
    67740 Da
    Quaternary structure:
    • Interacts with RHOA in its GTP- and GDP-bound states, and with CDC42 in its GTP-bound state. Interacts with the PDZ 1 domain of BAIAP1 (By similarity).
    • Sequence=AAB08847.1; Type=Frameshift; Positions=586; Evidence={ECO:0000305}; Sequence=AAB37683.1; Type=Frameshift; Positions=15, 33; Evidence={ECO:0000305}; Sequence=CAA08974.1; Type=Frameshift; Positions=8, 55, 59, 70, 586; Evidence={ECO:0000305};

    Three dimensional structures from OCA and Proteopedia for NET1 Gene

    Alternative splice isoforms for NET1 Gene


neXtProt entry for NET1 Gene

Proteomics data for NET1 Gene at MOPED

Post-translational modifications for NET1 Gene

  • Ubiquitination at Lys 194, Lys 301, and Lys 489
  • Modification sites at PhosphoSitePlus

Other Protein References for NET1 Gene

No data available for DME Specific Peptides for NET1 Gene

Domains & Families for NET1 Gene

Protein Domains for NET1 Gene

Graphical View of Domain Structure for InterPro Entry



  • Contains 1 DH (DBL-homology) domain.
  • Contains 1 DH (DBL-homology) domain.
  • Contains 1 PH domain.
genes like me logo Genes that share domains with NET1: view

Function for NET1 Gene

Molecular function for NET1 Gene

UniProtKB/Swiss-Prot Function:
Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase. May be involved in activation of the SAPK/JNK pathway Stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell death.
UniProtKB/Swiss-Prot Induction:
By TGFB1. Up-regulated by DNA damaging agents like H(2)O(2) or ionizing radiation (IR).
genes like me logo Genes that share phenotypes with NET1: view

Animal Models for NET1 Gene

MGI Knock Outs for NET1:

Animal Model Products

No data available for Enzyme Numbers (IUBMB) , Gene Ontology (GO) - Molecular Function , Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for NET1 Gene

Localization for NET1 Gene

Subcellular locations from UniProtKB/Swiss-Prot for NET1 Gene

Cytoplasm. Nucleus.

Subcellular locations from

Jensen Localization Image for NET1 Gene COMPARTMENTS Subcellular localization image for NET1 gene
Compartment Confidence
cytosol 5
nucleus 4
plasma membrane 2

No data available for Gene Ontology (GO) - Cellular Components for NET1 Gene

Pathways & Interactions for NET1 Gene

genes like me logo Genes that share pathways with NET1: view

Gene Ontology (GO) - Biological Process for NET1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0007165 signal transduction TAS 8649828
GO:0035023 regulation of Rho protein signal transduction IEA --
GO:0048011 neurotrophin TRK receptor signaling pathway TAS --
GO:0051056 regulation of small GTPase mediated signal transduction TAS --
GO:0051451 myoblast migration IEA --
genes like me logo Genes that share ontologies with NET1: view

No data available for SIGNOR curated interactions for NET1 Gene

Drugs & Compounds for NET1 Gene

(3) Drugs for NET1 Gene - From: HMDB

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Guanosine diphosphate Experimental Pharma 0
Guanosine monophosphate Experimental Pharma 0
Guanosine triphosphate Experimental Pharma 0
genes like me logo Genes that share compounds with NET1: view

Transcripts for NET1 Gene

Unigene Clusters for NET1 Gene

Neuroepithelial cell transforming 1:
Representative Sequences:

Alternative Splicing Database (ASD) splice patterns (SP) for NET1 Gene

ExUns: 1a · 1b · 1c ^ 2 ^ 3 ^ 4 ^ 5 ^ 6a · 6b · 6c ^ 7a · 7b ^ 8a · 8b · 8c ^ 9a · 9b ^ 10 ^ 11a · 11b ^ 12 ^ 13a · 13b ^ 14
SP1: - - - - - -
SP2: - - - -
SP3: - - - -
SP4: -
SP5: -
SP6: - -
SP7: - - - - - - - - -
SP8: -

Relevant External Links for NET1 Gene

GeneLoc Exon Structure for
ECgene alternative splicing isoforms for

Expression for NET1 Gene

mRNA expression in normal human tissues for NET1 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Protein differential expression in normal tissues from HIPED for NET1 Gene

This gene is overexpressed in Serum (56.5) and Cerebrospinal fluid (12.5).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MOPED, and MaxQB for NET1 Gene

SOURCE GeneReport for Unigene cluster for NET1 Gene Hs.25155

mRNA Expression by UniProt/SwissProt for NET1 Gene

Tissue specificity: Widely expressed.
genes like me logo Genes that share expression patterns with NET1: view

Protein tissue co-expression partners for NET1 Gene

Primer Products

No data available for mRNA differential expression in normal tissues for NET1 Gene

Orthologs for NET1 Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for NET1 Gene

Organism Taxonomy Gene Similarity Type Details
(Bos Taurus)
Mammalia NET1 35
  • 82.17 (n)
  • 82.36 (a)
NET1 36
  • 80 (a)
(Canis familiaris)
Mammalia NET1 35
  • 85.38 (n)
  • 86.59 (a)
NET1 36
  • 88 (a)
(Mus musculus)
Mammalia Net1 35
  • 85.32 (n)
  • 87.06 (a)
Net1 16
Net1 36
  • 87 (a)
(Pan troglodytes)
Mammalia NET1 35
  • 99.27 (n)
  • 99.33 (a)
NET1 36
  • 99 (a)
(Rattus norvegicus)
Mammalia Net1 35
  • 84.47 (n)
  • 87.08 (a)
(Monodelphis domestica)
Mammalia -- 36
  • 77 (a)
-- 36
  • 83 (a)
-- 36
  • 74 (a)
(Ornithorhynchus anatinus)
Mammalia NET1 36
  • 80 (a)
(Gallus gallus)
Aves NET1 35
  • 76.6 (n)
  • 80.78 (a)
NET1 36
  • 77 (a)
(Anolis carolinensis)
Reptilia NET1 36
  • 74 (a)
tropical clawed frog
(Silurana tropicalis)
Amphibia net1 35
  • 71.29 (n)
  • 74.82 (a)
Str.5900 35
(Danio rerio)
Actinopterygii net1 35
  • 64.94 (n)
  • 70.92 (a)
net1 36
  • 66 (a)
fruit fly
(Drosophila melanogaster)
Insecta Gef64C 36
  • 4 (a)
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes ROM1 36
  • 7 (a)
ROM2 36
  • 7 (a)
ROM1 38
sea squirt
(Ciona savignyi)
Ascidiacea -- 36
  • 46 (a)
Species with no ortholog for NET1:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for NET1 Gene

Gene Tree for NET1 (if available)
Gene Tree for NET1 (if available)

Paralogs for NET1 Gene

Paralogs for NET1 Gene

(8) SIMAP similar genes for NET1 Gene using alignment to 5 proteins: Pseudogenes for NET1 Gene

genes like me logo Genes that share paralogs with NET1: view

Variants for NET1 Gene

Sequence variations from dbSNP and Humsavar for NET1 Gene

SNP ID Clin Chr 10 pos Sequence Context AA Info Type
VAR_035972 A breast cancer sample
rs34658946 - 5,456,139(-) TCCGT(A/C/G)TGACG nc-transcript-variant, reference, missense
rs11791 -- 5,457,210(+) CTTGA(A/G)TTATT nc-transcript-variant, utr-variant-3-prime
rs1802554 -- 5,457,587(+) AACCT(G/T)CCGAT nc-transcript-variant, utr-variant-3-prime
rs3923347 -- 5,414,779(+) AGGCA(A/G)AAAAG intron-variant

Structural Variations from Database of Genomic Variants (DGV) for NET1 Gene

Variant ID Type Subtype PubMed ID
nsv894783 CNV Loss 21882294
nsv894792 CNV Gain 21882294
nsv831774 CNV Gain 17160897
esv2731718 CNV Deletion 23290073
dgv600n71 CNV Loss 21882294

Variation tolerance for NET1 Gene

Residual Variation Intolerance Score: 14.6% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 3.27; 52.88% of all genes are more intolerant (likely to be disease-causing)

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot and Relevant External Links for NET1 Gene

Disorders for NET1 Gene

MalaCards: The human disease database

(2) MalaCards diseases for NET1 Gene - From: OMIM and GeneCards

Disorder Aliases PubMed IDs
orthostatic intolerance
  • neurocirculatory asthenia
breast cancer
  • breast cancer, invasive ductal
- elite association - COSMIC cancer census association via MalaCards
Search NET1 in MalaCards View complete list of genes associated with diseases

Relevant External Links for NET1

Genetic Association Database (GAD)
Human Genome Epidemiology (HuGE) Navigator
Atlas of Genetics and Cytogenetics in Oncology and Haematology:
genes like me logo Genes that share disorders with NET1: view

No data available for UniProtKB/Swiss-Prot and Genatlas for NET1 Gene

Publications for NET1 Gene

  1. Isolation of a novel oncogene, NET1, from neuroepithelioma cells by expression cDNA cloning. (PMID: 8649828) Chan A.M.-L. … Miki T. (Oncogene 1996) 2 3 4 23 67
  2. Coexpression of alpha6beta4 integrin and guanine nucleotide exchange factor Net1 identifies node-positive breast cancer patients at high risk for distant metastasis. (PMID: 19124484) Gilcrease M.Z. … Frost J.A. (Cancer Epidemiol. Biomarkers Prev. 2009) 3 23
  3. Interaction of the RhoA exchange factor Net1 with discs large homolog 1 protects it from proteasome-mediated degradation and potentiates Net1 activity. (PMID: 19586902) Carr H.S. … Frost J.A. (J. Biol. Chem. 2009) 3 23
  4. Expression and clinical significance of NET-1 and PCNA in hepatocellular carcinoma. (PMID: 18214716) Shen S.Q. … Nakao A. (Med. Oncol. 2008) 3 23
  5. A bacterial cytotoxin identifies the RhoA exchange factor Net1 as a key effector in the response to DNA damage. (PMID: 18509476) Guerra L. … Frisan T. (PLoS ONE 2008) 3 23

Products for NET1 Gene

Sources for NET1 Gene