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Category Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21

GIFtS
-

NET1 Gene

protein-coding GIFtS: 63
GCID: GC10P005444

neuroepithelial cell transforming 1

Explore 11 diseases affiliated with
NET1 via

MalaCards

our new
Human Malady Compendium

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc, ⁷Ensembl, ⁸DME, ⁹miRBase, and/or ¹⁰fRNAdb)
About This Section

Aliases
Neuroepithelial Cell Transforming 1¹ ²		P65 Net1 Proto-Oncogene Protein²
ARHGEF8¹ ² ³		Rho Guanine Nucleotide Exchange Factor (GEF) 8²
NET1A¹ ²		Small GTP-Binding Protein Regulator²
Guanine Nucleotide Regulatory Protein (Oncogene)²		Proto-Oncogene P65 Net1³
Neuroepithelial Cell-Transforming Gene 1 Protein²		Rho Guanine Nucleotide Exchange Factor 8³
Neuroepithelioma Transforming Gene 1²

External Ids:	HGNC: 14592¹	Entrez Gene: 10276²	Ensembl: ENSG00000173848⁷	OMIM: 606450⁵	UniProtKB: Q7Z628³

Export aliases for NET1 gene to outside databases

Previous GC identifers: GC10M005513 GC10P005428 GC10P005554 GC10P005408

(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for NET1:

This gene is part of the family of Rho guanine nucleotide exchange factors. Members of this family activate Rho

proteins by catalyzing the exchange of GDP for GTP. The protein encoded by this gene interacts with RhoA within the

cell nucleus and may play a role in repairing DNA damage after ionizing radiation. Pseudogenes of this gene are

located on the long arms of chromosomes 1, 7 and 18. Alternative splicing results in multiple transcript variants that

encode different protein isoforms. (provided by RefSeq, Jul 2012)

UniProtKB/Swiss-Prot: ARHG8_HUMAN, Q7Z628

Function: Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase. May be involved in activation of the

SAPK/JNK pathway Stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell death

Gene Wiki entry for NET1

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 69), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section

RefSeq DNA sequence:

NC_000010.10 NC_018921.1 NT_008705.16

Regulatory elements:

SABiosciences Regulatory transcription factor binding sites in the NET1 gene promoter:
STAT5B AML1a GATA-3 Pax-2 Pax-2a Gfi-1 GATA-2 GATA-1
Other transcription factors

SwitchGear Promoter luciferase reporter plasmid: NET1 promoter sequence

Search SABiosciences Chromatin IP Primers for NET1

Epigenetics:

QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat NET1

Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 10p15 Ensembl cytogenetic band: 10p15.1 HGNC cytogenetic band: 10p15

NET1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
NET1 gene location

GeneLoc information about chromosome 10 GeneLoc Exon Structure

GeneLoc location for GC10P005444: view genomic region (about GC identifiers)

Start:	5,454,514 bp from pter	End:	5,500,426 bp from pter
Size:	45,913 bases	Orientation:	plus strand

(According to ¹UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to ²PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE MOPED and PaxDb, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Uscn,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Uscn, Ontologies according to Gene Ontology Consortium 01 Mar 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, Abcam, and/or Uscn)
About This Section

UniProtKB/Swiss-Prot: ARHG8_HUMAN, Q7Z628 (See protein sequence)

Recommended Name: Neuroepithelial cell-transforming gene 1 protein

Size: 596 amino acids; 67740 Da

Subunit: Interacts with RHOA in its GTP- and GDP-bound states, and with CDC42 in its GTP-bound state. Interacts with

the PDZ 1 domain of BAIAP1 (By similarity)

Subcellular location: Cytoplasm (By similarity). Nucleus (By similarity)

Sequence caution: Sequence=AAB08847.1; Type=Frameshift; Positions=586; Sequence=AAB37683.1; Type=Frameshift;

Positions=15, 33; Sequence=CAA08974.1; Type=Frameshift; Positions=8, 55, 59, 70, 586;

1 PDB 3D structure from and Proteopedia for NET1:

3EO2 (3D)

Secondary accessions: Q12773 Q96D82 Q99903 Q9UEN6

Alternative splicing: 2 isoforms: Q7Z628-1 Q7Z628-2

Explore the universe of human proteins at neXtProt for NET1: NX_Q7Z628

Post-translational modifications:

View modification sites using PhosphoSitePlus²

View neXtProt modification sites for NX_Q7Z628

NET1 Protein expression data from MOPED and PaxDb: About this image

REFSEQ proteins (2 alternative transcripts):

NP_001040625.1 NP_005854.2

ENSEMBL proteins:

ENSP00000347134 ENSP00000403101 ENSP00000369717 ENSP00000446452

Reactome Protein details: Q7Z628
Human Recombinant Protein Products:

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	GenScript Custom Purified and Recombinant Proteins Services for NET1
	Novus Biologicals NET1 Proteins Novus Biologicals NET1 Lysates
	Browse Sino Biological Recombinant Proteins
	Browse ProSpec Recombinant Proteins
	Uscn Proteins for NET1

Gene Ontology (GO): 3 cellular component terms (GO ID links to tree view): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005622	intracellular	--	--
GO:0005634	nucleus	IEA	--
GO:0005829	cytosol	TAS	--

NET1 for ontologies About GeneDecksing

NET1 Antibody Products:

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	Novus Biologicals NET1 Antibodies
	Search for Antibodies for NET1 at Abcam
	Uscn Antibodies for NET1
	ThermoFisher Antibodies for NET1

Assay Products for NET1:

	Browse Kits and Assays available from EMD Millipore
	OriGene Custom Immunoassay Development Browse OriGene Fluorogenic Cell Assay Kits
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	GenScript Custom Assay Services for NET1
	Browse Enzo Life Sciences for kits & assays
	Uscn ELISAs and CLIAs for NET1

(According to InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

NET1 for domains About GeneDecksing

5 InterPro domains/families:

IPR001331 GDS_CDC24_CS

IPR000219 DH-domain

IPR001849 Pleckstrin_homology

IPR011993 PH_like_dom

IPR015721 RhoGEF-like

Graphical View of Domain Structure for InterPro Entry Q7Z628

ProtoNet protein and cluster: Q7Z628

2 Blocks protein families:

IPB000219 DH domain

IPB001849 Pleckstrin-like

UniProtKB/Swiss-Prot: ARHG8_HUMAN, Q7Z628

Similarity: Contains 1 DH (DBL-homology) domain

Similarity: Contains 1 PH domain

(According to ¹UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or ²DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013,
bound targets from SABiosciences, miRNA Gene Targets from miRTarBase shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene.)
About This Section

Function Summary:

UniProtKB/Swiss-Prot: ARHG8_HUMAN, Q7Z628

Function: Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase. May be involved in activation of the

SAPK/JNK pathway Stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell death

Induction: By TGFB1. Up-regulated by DNA damaging agents like H(2)O(2) or ionizing radiation (IR)

miRNA Products:		OriGene 3'-UTR Clone (see all 2): NET1 Browse MicroRNA Expression Plasmids
		QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat NET1
		8/23 QIAGEN miScript miRNA Assays for microRNAs that regulate NET1 (see all 23):
		hsa-miR-199b-3p hsa-miR-199a-3p hsa-miR-657 hsa-miR-3129-5p hsa-miR-132 hsa-miR-340 hsa-miR-144 hsa-miR-548x

		SwitchGear 3'UTR luciferase reporter plasmid: NET1 3' UTR sequence

Inhib. RNA Products:		Browse for Gene Knock-down Tools from EMD Millipore
		OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for NET1 (see all 7) OriGene shRNA RFP: NET1 OriGene siRNA: NET1
		QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat NET1

Gene Editing
Products:

DNA2.0 Custom Protein Engineering Service for NET1

Clone Products:		Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore
		OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for NET1 (see all 5) OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for NET1 (see all 2) OriGene custom cloning services � gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
		GenScript: all cDNA clones in your preferred vector (see all 2): NET1 (NM_005863)
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		DNA2.0 Custom Codon Optimized Gene Synthesis Service for NET1
		Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat NET1

Cell Line Products:		GenScript Custom overexpressing Cell Line Services for NET1
		Search LifeMap BioReagents cell lines for NET1

In Situ Assay
Products:

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for NET1

Gene Ontology (GO): 3 molecular function terms (GO ID links to tree view): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005085	guanyl-nucleotide exchange factor activity	TAS	8649828
GO:0005089	Rho guanyl-nucleotide exchange factor activity	IEA	--
GO:0005543	phospholipid binding	IEA	--

NET1 for ontologies About GeneDecksing

3 GenomeRNAi human phenotypes for NET1:

Decreased focal adhesion (FA)

Synthetic lethal with Ras

Upregulation of Wnt/beta-caten

(Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to ¹UniProtKB, ²MINT, ³I2D, and/or ⁴STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Mar 2013 via Entrez Gene).
About This Section

Unified GeneCards pathways - 5/19 super-pathways (see all 19) About this table

See pathways by source

Super-pathway

contained gene-specific pathways

Cell death signalling via NRAGE, NRIF and NADE

Cell death signalling via NRAGE, NRIF and NADE	1.00	G alpha (12/13) signalling events	0.39
NRAGE signals death through JNK	0.74	Rho GTPase cycle	0.25
p75 NTR receptor-mediated signalling	0.73	Signaling by Rho GTPases	0.25

GPCR Pathway

	GPCR Pathway	1.00			Breast Cancer Regulation by Stathmin1	0.58
	Ras Pathway	0.62			Pancreatic Adenocarcinoma	0.55

G-AlphaQ Signaling

	G-AlphaQ Signaling	1.00			Thrombin Signaling	0.40
	G-AlphaI Signaling	0.47

Signaling by GPCR

	Signaling by GPCR	1.00			Signal Transduction	0.56
	GPCR downstream signaling	0.89

Rho Family GTPases

Rho Family GTPases

1.00

Molecular Mechanisms of Cancer

0.51

Pathway sources
See GeneCards unified pathways
Show all pathways

1 EMD Millipore Pathway for NET1

Cytoskeleton remodeling Role of PDGFs in cell migration

5/21 Downloadable PowerPoint Slides of QIAGEN Pathway Central Maps for NET1 (see all 21)

	RhoA Pathway
	Guidance Cues and Growth Cone Motility
	Molecular Mechanisms of Cancer
	Interferon Pathway
	G-AlphaI Signaling

1 GeneGo (Thomson Reuters) Pathway for NET1

Cytoskeleton remodeling Role of PDGFs in cell migration

1 BioSystems Pathway for NET1

Regulation of RhoA activity

5/10 Reactome Pathways for NET1 (see all 10)

	GPCR downstream signaling
	Signaling by Rho GTPases
	G alpha (12/13) signalling events
	Cell death signalling via NRAGE, NRIF and NADE
	Signaling by GPCR

NET1 for pathways About GeneDecksing

Interactions:

SABiosciences Gene Network Central^TM Interacting Genes and Proteins Network for NET1

STRING Interaction Network Preview (showing 5 interactants - click image to see 9)

5/11 Interacting proteins for NET1 (Q7Z628³ ENSP00000347134⁴) via UniProtKB, MINT, STRING, and/or I2D (see all 11)

Interactant		Interaction Details
GeneCard	External ID(s)	Interaction Details
PRKAB2	O43741³, ENSP00000254101⁴	I2D: score=3 STRING: ENSP00000254101
DLG1	Q12959³, ENSP00000345731⁴	I2D: score=1 STRING: ENSP00000345731
RHOA	P61586³, ENSP00000400175⁴	I2D: score=1 STRING: ENSP00000400175
MAGI1	Q96QZ7³, ENSP00000385450⁴	I2D: score=3 STRING: ENSP00000385450
UBC	P0CG48³, ENSP00000344818⁴	I2D: score=1 STRING: ENSP00000344818

About this table

Gene Ontology (GO): 5/12 biological process terms (GO ID links to tree view) (see all 12): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0001558	regulation of cell growth	NAS	8649828
GO:0006915	apoptotic process	TAS	--
GO:0007165	signal transduction	TAS	8649828
GO:0007264	small GTPase mediated signal transduction	TAS	--
GO:0032321	positive regulation of Rho GTPase activity	IMP	--

NET1 for ontologies About GeneDecksing

(Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
About This Section

Browse Small Molecules at EMD Millipore

Browse drugs & compounds from Enzo Life Sciences

Browse Tocris compounds for NET1

3 HMDB Compounds for NET1 About this table

Compound	Synonyms	CAS #	PubMed Ids
Guanosine diphosphate	5'-GDP (see all 10)	146-91-8	--
Guanosine monophosphate	5'-GMP (see all 14)	85-32-5	--
Guanosine triphosphate	5'-GTP (see all 10)	86-01-1	--

Search CenterWatch for drugs/clinical trials and news about NET1 / ARHG8

(Secondary structures according to fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from EMD Millipore,
siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, SABiosciences, and/or QIAGEN )
About This Section

REFSEQ mRNAs for NET1 gene (2 alternative transcripts):

NM_001047160.2 NM_005863.4

Unigene Cluster for NET1:

Neuroepithelial cell transforming 1

Hs.25155 [show with all ESTs]

Unigene Representative Sequence: NM_001047160

7 Ensembl transcripts including schematic representations, and UCSC links where relevant:

ENST00000355029(uc001iia.3 uc010qar.2) ENST00000486354 ENST00000449083

ENST00000380359(uc001iib.3 uc010qas.2) ENST00000465087 ENST00000484741

ENST00000542715

miRNA Products:		OriGene 3'-UTR Clone (see all 2): NET1 Browse OriGene MicroRNA Expression Plasmids
		QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat NET1
		8/23 QIAGEN miScript miRNA Assays for microRNAs that regulate NET1 (see all 23):
		hsa-miR-199b-3p hsa-miR-199a-3p hsa-miR-657 hsa-miR-3129-5p hsa-miR-132 hsa-miR-340 hsa-miR-144 hsa-miR-548x

		SwitchGear 3'UTR luciferase reporter plasmid: NET1 3' UTR sequence

Inhib. RNA Products:		Browse for Gene Knock-down Tools from EMD Millipore
		OriGene 29mer shRNA kits in GFP-retroviral vector in human, mouse, rat for NET1 (see all 7) OriGene shRNA RFP: NET1 OriGene siRNA: NET1
		QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat NET1

Clone Products:		OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for NET1 (see all 5) OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for NET1 (see all 2) OriGene custom cloning services � gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
		GenScript: all cDNA clones in your preferred vector (see all 2): NET1 (NM_005863)
		DNA2.0 Custom Codon Optimized Gene Synthesis Service for NET1
		Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat NET1

Primer Products:		OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for NET1 Browse OriGene validated miRNA SYBR primer pairs
		SABiosciences RT² qPCR Primer Assay in human, mouse, rat NET1
		QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat NET1
		QIAGEN QuantiFast Probe-based Assays in human, mouse, rat NET1

Additional cDNA sequence:

AB209852.1 AJ010046.1 AK024919.1 AK294244.1 AK299335.1 AK302687.1 AK304028.1 AK315566.1

BC010285.1 BC053553.1 NR_073040.1 S82401.1 U02081.1

18 DOTS entries:

DT.97860210 DT.455207 DT.86838054 DT.99991536 DT.100792472 DT.455206 DT.97810157 DT.121221194

DT.102826903 DT.121221244 DT.95301494 DT.40279022 DT.92447449 DT.121221239 DT.121221261 DT.447089

DT.92384021 DT.97860209

24/386 AceView cDNA sequences (see all 386):

CB119951 BQ024362 AU125526 AU130957 AA831287 BM767301 CA391308 BG770848

BQ181013 AU280423 BC053553 CA394283 BQ024592 AW771003 BM783408 AA062769

BM835259 AW631462 AL079853 CB149941 BP344840 AW771396 BM854468 BM822189

GeneLoc Exon Structure

5/10 Alternative Splicing Database (ASD) splice patterns (SP) for NET1 (see all 10) About this scheme

ExUns:	1a	·	1b	·	1c	^	2	^	3	^	4	^	5	^	6a	·	6b	·	6c	^	7a	·	7b	^	8a	·	8b	·	8c	^	9a	·	9b	^	10	^	11a	·	11b	^	12	^	13a	·	13b	^	14
SP1:											-		-						-				-		-																				-
SP2:																			-				-		-																				-
SP3:											-		-						-				-
SP4:													-
SP5:																																													-

ECgene alternative splicing isoforms for NET1

(RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section

NET1 expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: AAATGGCTTG

About this image

NET1 expression in embryonic tissues and stem cells

Expression by the Database of Embryonic development, Stem cell research, and Regenerative medicine About this table

1 LifeMap In Vivo Development Anatomical Compartment/Cell

Tissue

Anatomical Compartment

Cell

Category (developmental path)

Limb

Limb Bud

Limb Bud Mesenchyme Cells

Mesoderm

Expression:

Positive

Negative

Selective marker

Experimental details:

Curated

Microarrays

In-situ hybridization

Stem Cell Differentiation: 1 LifeMap Cell

Name

Category

PureStem™ progenitor W10 (Embryonic Progenitor Cell)

See NET1 Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for NET1

SOURCE GeneReport for Unigene cluster: Hs.25155

UniProtKB/Swiss-Prot: ARHG8_HUMAN, Q7Z628

Tissue specificity: Widely expressed

SABiosciences Custom PCR Arrays for NET1

Primer Products:		OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for NET1 Browse OriGene validated miRNA SYBR primer pairs
		SABiosciences RT² qPCR Primer Assay in human, mouse, rat NET1
		QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat NET1
		QIAGEN QuantiFast Probe-based Assays in human, mouse, rat NET1

In Situ
Assay Products:

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for NET1

(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD , ⁵MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or ⁶Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
About This Section

This gene was present in the common ancestor of animals.

Orthologs for NET1 gene from 5/20 species (see all 20) About this table

Organism	Taxonomic classification	Gene	Description	Human Similarity	Orthology Type	Details
chicken (Gallus gallus)	Aves	NET1¹	neuroepithelial cell transforming 1	75.21(n) 78.94(a)		416693 NM_001030648.1 NP_001025819.1
lizard (Anolis carolinensis)	Reptilia	NET1⁶	--	76(a)	1 ↔ 1	GL343728.1(90744-108707)
African clawed frog (Xenopus laevis)	Amphibia	BC042313.1²	--	78.49(n)		BC042313.1
zebrafish (Danio rerio)	Actinopterygii	net1¹	neuroepithelial cell transforming gene 1	64.15(n) 69.05(a)		493607 NM_001007767.1 NP_001007768.1
honey bee (Apis mellifera)	Insecta	--	--	22(a)	1 → many	Group1.48(27406-29435)

ENSEMBL Gene Tree for NET1 (if available)
TreeFam Gene Tree for NET1 (if available)

(Paralogs according to ¹HomoloGene,
²Ensembl, and ³SIMAP, Pseudogenes according to ⁴Pseudogene.org Build 68)
About This Section

Paralogs for NET1 gene

ARHGEF2² ARHGEF3² ARHGEF11² ARHGEF1² ARHGEF12² ARHGEF18² AKAP13² ARHGEF28²

8 SIMAP similar genes for NET1 using alignment to 5 protein entries: ARHG8_HUMAN (see all proteins):

ARHGEF3 BCR/ABL fusion PREX1 ITSN1 PREX2 ARHGEF12

ARHGEF1 ITSN2

NET1 for paralogs About GeneDecksing

3 Pseudogenes.org Pseudogenes for NET1
PGOHUM00000235063 PGOHUM00000245126 PGOHUM00000233408

(SNPs/Variants according to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section

SNP ID	Valid	Clinical significance	Chr 10 pos	Sequence	#	AA Chg	Type	More	#	Allele freq	Pop	Total sample	More
Genomic Data					Transcription Related Data				Allele Frequencies
				--	--	--		--	--
rs148736059^1,2	C,	--	5452532(+)	`CACTC-/TTAACT`	1	--	us2k¹		0	--	--	--	--
rs139034646^1,2		--	5452584(+)	`ATGGGA/GAAGGT`	1	--	us2k¹		0	--	--	--	--
rs182992019^1,2		--	5452644(+)	`CCTTCC/TGGTGG`	1	--	us2k¹		0	--	--	--	--
rs187357396^1,2		--	5452743(+)	`GAGCAG/TCAGCA`	1	--	us2k¹		0	--	--	--	--
rs60713184^1,2	C,F,	--	5452758(+)	`TTATTG/ATAAAG`	1	--	us2k¹		3		WA	122
rs191050513^1,2		--	5452793(+)	`GTGTGA/GAAGGG`	1	--	us2k¹		0	--	--	--	--
rs75829724^1,2	C	--	5452799(+)	`CAGGGT/GACCCG`	1	--	us2k¹		1		NA	2
rs184093748^1,2		--	5452898(+)	`TTTTTA/TCAATC`	1	--	us2k¹		0	--	--	--	--
rs188596056^1,2		--	5453094(+)	`TCAATG/TTTACT`	1	--	us2k¹		0	--	--	--	--
rs112503350^1,2		--	5453142(+)	`TCCCTC/TATGTT`	1	--	us2k¹		2		CSA WA	120

HapMap Linkage Disequilibrium report for NET1 (5454514 - 5500426 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV): 3 variations for NET1
3 CNVs: 75086 85087 85088

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(in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
About This Section

NET1 for disorders           About GeneDecksing

OMIM gene information: 606450    OMIM disorders: --

11 diseases for NET1:    About MalaCards

neuroepithelioma attention deficit hyperactivity disorder adhd hepatocellular carcinoma

alzheimer's disease gastric cancer breast cancer carcinoma

leukemia adenocarcinoma pancreatitis

1 Novoseek disease relationship for NET1 gene About this table

Disease	-log (P-Val)	Hits	PubMed IDs for Articles with Shared Sentences (# sentences)
neuroepithelioma	87.5	3	15611121 (1), 19124484 (1), 8649828 (1)

Genetic Association Database (GAD): NET1
Human Genome Epidemiology (HuGE) Navigator: NET1 (97 documents)

Export disorders for NET1 gene to outside databases

(in PubMed. Associations of this gene to articles via ¹Entrez Gene, ²UniProtKB/Swiss-Prot, ³HGNC, ⁴GAD, ⁵PharmGKB, ⁶HMDB, ⁷DrugBank, ⁸UniProtKB/TrEMBL, ⁹ Novoseek, and/or ¹⁰fRNAdb)
About This Section

PubMed articles for NET1 gene, integrated from 9 sources (see all 46):
(articles sorted by number of sources associating them with NET1)

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Isolation of a novel oncogene, NET1, from neuroepithelioma cells by expression cDNA cloning. (PubMed id 8649828)^{1, 2, 3, 9} Chan A.M.-L.... Miki T. (1996)
The nuclear guanine nucleotide exchange factors Ect2 and Net1 regulate RhoB-mediated cell death after DNA damage. (PubMed id 21373644)^{1, 2} Srougi M.C. and Burridge K. (2011)
Support for association between ADHD and two candidate genes: NET1 and DRD1. (PubMed id 15717291)^{1, 4} Bobb A.J....Rapoport J.L. (2005)
DNA pooling analysis of 21 norepinephrine transporter gene SNPs with attention deficit hyperactivity disorder: no evidence for association. (PubMed id 15719398)^{1, 4} Xu X....Asherson P. (2005)
The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)^{1, 2} Gerhard D.S....Malek J. (2004)
The activity of guanine exchange factor NET1 is essential for transforming growth factor-beta-mediated stress fiber formation. (PubMed id 11278519)^{1, 2} Shen X.... Wang X.-F. (2001)
Interaction of the RhoA exchange factor Net1 with dis cs large homolog 1 protects it from proteasome-mediated degradation and potenti ates Net1 activity. (PubMed id 19586902)^{1, 9} Carr H.S....Frost J.A. (2009)
Expression and clinical significance of NET-1 and PCNA in hepatocellular carcinoma. (PubMed id 18214716)^{1, 9} Shen S.Q....Nakao A. (2008)
Characterization of the biochemical and transforming properties of the neuroepithelial transforming protein 1. (PubMed id 15611121)^{1, 9} Qin H....Frost J.A. (2005)
The nuclear RhoA exchange factor Net1 interacts with proteins of the Dlg family, affects their localization, and influences their tumor suppressor activity. (PubMed id 17938206)^{1, 9} Garcia-Mata R....Burridge K. (2007)

(in PubMed, OMIM, and NCBI Bookshelf)
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		AND	OR
Aliases
Disorders
Free Text

Query String		PubMed OMIM NCBI Bookshelf
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(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
About This Section

Entrez Gene: 10276	HGNC: 14592	AceView: NET1	Ensembl:ENSG00000173848	euGenes: HUgn10276
ECgene: NET1	H-InvDB: NET1

(According to HUGE)
About This Section

(According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

Name	Description
PharmGKB entry for NET1	Pharmacogenomics, SNPs, Pathways
ATLAS Chromosomes in Cancer entry for NET1	Genetics and Cytogenetics in Oncology and Haematology

Intellectual Property for NET1 gene
(Patent information from GeneIP, Licensable technologies from WIS Yeda, Salk, Tufts, IP news from LifeMap Sciences, Inc.)

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Patent Information for NET1 gene:
Search GeneIP for patents involving NET1

GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search

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neuroepithelial cell transforming 1

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neuroepithelial cell transforming 1

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