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Set Analyses:

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NET1 Gene

protein-coding GIFtS: 66
GCID: GC10P005444

Neuroepithelial Cell Transforming 1

Alzheimer's & Parkinson's Diseases Congress

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at

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(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc, ⁷Ensembl, ⁸DME, ⁹miRBase, ¹⁰fRNAdb, ¹²H-InvDB, ¹³NCBI, ¹⁴NONCODE, and/or ¹⁵RNAdb)
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Aliases
Neuroepithelial Cell Transforming 1¹ ²		P65 Net1 Proto-Oncogene Protein²
ARHGEF8² ³		Rho Guanine Nucleotide Exchange Factor (GEF) 8²
NET1A²		Small GTP-Binding Protein Regulator²
Guanine Nucleotide Regulatory Protein (Oncogene)²		Proto-Oncogene P65 Net1³
Neuroepithelial Cell-Transforming Gene 1 Protein²		Rho Guanine Nucleotide Exchange Factor 8³
Neuroepithelioma Transforming Gene 1²

External Ids:	HGNC: 14592¹	Entrez Gene: 10276²	Ensembl: ENSG00000173848⁷	OMIM: 606450⁵	UniProtKB: Q7Z628³

Export aliases for NET1 gene to outside databases

Previous GC identifers: GC10M005513 GC10P005428 GC10P005554 GC10P005408

(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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Entrez Gene summary for NET1 Gene:

This gene is part of the family of Rho guanine nucleotide exchange factors. Members of this family activate Rho

proteins by catalyzing the exchange of GDP for GTP. The protein encoded by this gene interacts with RhoA within

the cell nucleus and may play a role in repairing DNA damage after ionizing radiation. Pseudogenes of this gene

are located on the long arms of chromosomes 1, 7 and 18. Alternative splicing results in multiple transcript

variants that encode different protein isoforms. (provided by RefSeq, Jul 2012)

GeneCards Summary for NET1 Gene:

NET1 (neuroepithelial cell transforming 1) is a protein-coding gene. Diseases associated with NET1 include slc6a5-related hyperekplexia, and orthostatic intolerance. GO annotations related to this gene include phospholipid binding and Rho guanyl-nucleotide exchange factor activity. An important paralog of this gene is ARHGEF2.

UniProtKB/Swiss-Prot: ARHG8_HUMAN, Q7Z628

Function: Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase. May be involved in activation of the

SAPK/JNK pathway Stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell

death

Gene Wiki entry for NET1 Gene

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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RefSeq DNA sequence:

NC_000010.11 NC_018921.2 NT_008705.17

Regulatory elements:

Regulatory transcription factor binding sites in the NET1 gene promoter:
STAT5B AML1a GATA-3 Pax-2 Pax-2a Gfi-1 GATA-2 GATA-1
Other transcription factors

SwitchGear Promoter luciferase reporter plasmid: NET1 promoter sequence

Search Chromatin IP Primers for NET1

Epigenetics:

DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat NET1

Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 10p15 Ensembl cytogenetic band: 10p15.1 HGNC cytogenetic band: 10p15

NET1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
NET1 gene location

GeneLoc information about chromosome 10 GeneLoc Exon Structure

GeneLoc location for GC10P005444: view genomic region (about GC identifiers)

Start:	5,454,514 bp from pter	End:	5,500,426 bp from pter
Size:	45,913 bases	Orientation:	plus strand

(According to ¹UniProtKB, HORDE, ²neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Cloud-Clone Corp.,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Cloud-Clone Corp., Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, and/or Cloud-Clone Corp.)
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UniProtKB/Swiss-Prot: ARHG8_HUMAN, Q7Z628 (See protein sequence)

Recommended Name: Neuroepithelial cell-transforming gene 1 protein

Size: 596 amino acids; 67740 Da

Subunit: Interacts with RHOA in its GTP- and GDP-bound states, and with CDC42 in its GTP-bound state. Interacts

with the PDZ 1 domain of BAIAP1 (By similarity)

Sequence caution: Sequence=AAB08847.1; Type=Frameshift; Positions=586; Sequence=AAB37683.1; Type=Frameshift;

Positions=15, 33; Sequence=CAA08974.1; Type=Frameshift; Positions=8, 55, 59, 70, 586;

1 PDB 3D structure from and Proteopedia for NET1:

3EO2 (3D)

Secondary accessions: Q12773 Q96D82 Q99903 Q9UEN6

Alternative splicing: 2 isoforms: Q7Z628-1 Q7Z628-2

Explore the universe of human proteins at neXtProt for NET1: NX_Q7Z628

Explore proteomics data for NET1 at MOPED

Post-translational modifications:

Ubiquitination² at Lys194, Lys301, Lys489

Modification sites at PhosphoSitePlus

See NET1 Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

REFSEQ proteins (2 alternative transcripts):

NP_001040625.1 NP_005854.2

ENSEMBL proteins:

ENSP00000347134 ENSP00000403101 ENSP00000369717 ENSP00000446452

Reactome Protein details: Q7Z628

NET1 Human Recombinant Protein Products:

	Browse Purified and Recombinant Proteins at EMD Millipore
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	Browse recombinant and purified proteins available from Enzo Life Sciences
	OriGene Purified Protein for NET1 OriGene Protein Over-expression Lysate for NET1 OriGene Custom MassSpec OriGene Custom Protein Services for NET1
	GenScript Custom Purified and Recombinant Proteins Services for NET1
	Novus Biologicals NET1 Protein Novus Biologicals NET1 Lysates
	Browse Sino Biological Recombinant Proteins Browse Sino Biological Cell Lysates
	Browse ProSpec Recombinant Proteins
	Cloud-Clone Corp. Proteins for NET1

NET1 Antibody Products:

	Browse EMD Millipore's Extensive Line of Mono- and Polyclonal Antibodies
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	OriGene Antibodies for NET1 OriGene Custom Antibody Services for NET1
	Novus Biologicals NET1 Antibodies
	Abcam antibodies for NET1 (Q7Z628, Q9UEN6)
	Cloud-Clone Corp. Antibodies for NET1
	ThermoFisher Antibodies for NET1
	LSBio Antibodies in human, mouse, rat for NET1

NET1 Assay Products:

	Browse Kits and Assays available from EMD Millipore
	OriGene Custom Assay Services for NET1
	Browse R&D Systems for biochemical assays
	GenScript Custom Assay Services for NET1
	Browse Enzo Life Sciences for kits & assays
	Cloud-Clone Corp. ELISAs for NET1 Cloud-Clone Corp. CLIAs for NET1

(According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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HGNC Gene Families:

PLEKH: Pleckstrin homology (PH) domain containing

ARHGEF: Rho guanine nucleotide exchange factors

5 InterPro protein domains:

IPR000219 DH-domain

IPR001849 Pleckstrin_homology

IPR011993 PH_like_dom

IPR015721 RhoGEF-like

IPR001331 GDS_CDC24_CS

Graphical View of Domain Structure for InterPro Entry Q7Z628

ProtoNet protein and cluster: Q7Z628

2 Blocks protein domains:

IPB000219 DH domain

IPB001849 Pleckstrin-like

UniProtKB/Swiss-Prot: ARHG8_HUMAN, Q7Z628

Similarity: Contains 1 DH (DBL-homology) domain

Similarity: Contains 1 PH domain

NET1 for domains About GeneDecksing

(According to ¹UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or ²DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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Molecular Function:

UniProtKB/Swiss-Prot Summary: ARHG8_HUMAN, Q7Z628

Function: Acts as guanine nucleotide exchange factor (GEF) for RhoA GTPase. May be involved in activation of the

SAPK/JNK pathway Stimulates genotoxic stress-induced RHOB activity in breast cancer cells leading to their cell

death

Induction: By TGFB1. Up-regulated by DNA damaging agents like H(2)O(2) or ionizing radiation (IR)

Gene Ontology (GO): 3 molecular function terms: About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005085	guanyl-nucleotide exchange factor activity	TAS	8649828
GO:0005089	Rho guanyl-nucleotide exchange factor activity	IEA	--
GO:0005515	protein binding	--	--

NET1 for ontologies About GeneDecksing

Phenotypes:
3 GenomeRNAi human phenotypes for NET1:

Decreased focal adhesion (FA)

Synthetic lethal with Ras

Upregulation of Wnt/beta-caten

Animal Models:

inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for NET1

inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for NET1

genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for NET1

genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for NET1

miRNA Products:		miRTarBase miRNAs that target NET1: hsa-mir-340-5p (MIRT019642), hsa-mir-320a (MIRT044778), hsa-mir-24-3p (MIRT030610), hsa-mir-26b-5p (MIRT029990)

		Block miRNA regulation of human, mouse, rat NET1 using miScript Target Protectors
		Selected qRT-PCR Assays for microRNAs that regulate NET1 (see all 23):
		hsa-miR-199b-3p hsa-miR-199a-3p hsa-miR-657 hsa-miR-3129-5p hsa-miR-132 hsa-miR-340 hsa-miR-144 hsa-miR-548x

		SwitchGear 3'UTR luciferase reporter plasmid: NET1 3' UTR sequence

Inhib. RNA Products:		OriGene RNAi products in human, mouse, rat for NET1
		Predesigned siRNA for gene silencing in human, mouse, rat NET1

Gene Editing
Products:

DNA2.0 Custom Protein Engineering Service for NET1

Clone Products:		OriGene clones in human, mouse for NET1 (see all 12) OriGene ORF clones in mouse, rat for NET1 OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
		GenScript: all cDNA clones in your preferred vector (see all 2): NET1 (NM_005863)
		Sino Biological Human cDNA Clone for NET1
		DNA2.0 Custom Codon Optimized Gene Synthesis Service for NET1
		Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat NET1

Cell Line Products:		GenScript Custom overexpressing Cell Line Services for NET1
		Browse ESI BIO Cell Lines and PureStem Progenitors for NET1

In Situ Assay
Products:

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for NET1

(According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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Subcellular locations from UniProtKB/Swiss-Prot

ARHG8_HUMAN, Q7Z628: Cytoplasm (By similarity). Nucleus (By similarity)

Subcellular locations from COMPARTMENTS:

Compartment	Confidence
cytosol	4
nucleus	2

About this image

Gene Ontology (GO): 2 cellular component terms: About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005634	nucleus	IEA	--
GO:0005829	cytosol	TAS	--

NET1 for ontologies About GeneDecksing

(SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to ¹UniProtKB, ²MINT, ³I2D, and/or ⁴STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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SuperPaths for NET1 About (see all 20)

See pathways by source

SuperPath

Contained pathways About

p75 NTR receptor-mediated signalling

	p75 NTR receptor-mediated signalling	0.74			NRAGE signals death through JNK	0.71
	Cell death signalling via NRAGE, NRIF and NADE	0.74			G alpha (12/13) signalling events	0.44

GPCR Pathway

	Ras Pathway	0.73			Pancreatic Adenocarcinoma	0.59
	GPCR Pathway	0.62			Breast Cancer Regulation by Stathmin1	0.58

Signaling by GPCR

	Signaling by GPCR	0.90			Signal Transduction	0.58
	GPCR downstream signaling	0.90

G-AlphaQ Signaling

	G-AlphaQ Signaling	0.47			Thrombin Signaling	0.40
	G-AlphaI Signaling	0.47

ERK Signaling

Rho Family GTPases

0.61

Molecular Mechanisms of Cancer

0.51

Pathways by source See SuperPaths
Show all pathways

Selected Downloadable PowerPoint Slides of GeneGlobe Pathway Central Maps for NET1 (see all 21)

	RhoA Pathway
	Guidance Cues and Growth Cone Motility
	Molecular Mechanisms of Cancer
	Interferon Pathway
	G-AlphaI Signaling

1 GeneGo (Thomson Reuters) Pathway for NET1

Cytoskeleton remodeling Role of PDGFs in cell migration

1 BioSystems Pathway for NET1

Regulation of RhoA activity

3 Reactome Pathways for NET1

	G alpha (12/13) signalling events
	NRAGE signals death through JNK
	Rho GTPase cycle

NET1 for pathways About GeneDecksing

Custom Pathway & Disease-focused RT² Profiler PCR Arrays for NET1
Interactions:

GeneGlobe Interaction Network for NET1

STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

Selected Interacting proteins for NET1 (Q7Z628³ ENSP00000347134⁴) via UniProtKB, MINT, STRING, and/or I2D (see all 53)

Interactant		Interaction Details
GeneCard	External ID(s)	Interaction Details
PRKAB2	O43741³, ENSP00000254101⁴	I2D: score=3 STRING: ENSP00000254101
DLG1	Q12959³, ENSP00000345731⁴	I2D: score=1 STRING: ENSP00000345731
RHOA	P61586³, ENSP00000400175⁴	I2D: score=1 STRING: ENSP00000400175
MAGI1	Q96QZ7³, ENSP00000385450⁴	I2D: score=3 STRING: ENSP00000385450
UBC	P0CG48³, ENSP00000344818⁴	I2D: score=1 STRING: ENSP00000344818

About this table

Gene Ontology (GO): Selected biological process terms (see all 12): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0001558	regulation of cell growth	NAS	8649828
GO:0007165	signal transduction	TAS	8649828
GO:0007264	small GTPase mediated signal transduction	TAS	--
GO:0032321	positive regulation of Rho GTPase activity	IMP	--
GO:0035023	regulation of Rho protein signal transduction	--	--

NET1 for ontologies About GeneDecksing

(Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
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Browse Small Molecules at EMD Millipore

Browse drugs & compounds from Enzo Life Sciences

Browse compounds at ApexBio

Browse Tocris compounds for NET1 (ARHG8)

3 HMDB Compounds for NET1 About this table

Compound	Synonyms	CAS #	PubMed Ids
Guanosine diphosphate	5'-GDP (see all 10)	146-91-8	--
Guanosine monophosphate	5'-GMP (see all 14)	85-32-5	--
Guanosine triphosphate	5'-GTP (see all 10)	86-01-1	--

(Secondary structures according to fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
Conferences by KenesGroup, exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, and/or QIAGEN )
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REFSEQ mRNAs for NET1 gene (2 alternative transcripts):

NM_001047160.2 NM_005863.4

Unigene Cluster for NET1:

Neuroepithelial cell transforming 1

Hs.25155 [show with all ESTs]

Unigene Representative Sequence: NM_001047160

7 Ensembl transcripts including schematic representations, and UCSC links where relevant:

ENST00000355029(uc001iia.3 uc010qar.2) ENST00000486354 ENST00000449083

ENST00000380359(uc001iib.3 uc010qas.2) ENST00000465087 ENST00000484741

ENST00000542715

miRNA Products:		Block miRNA regulation of human, mouse, rat NET1 using miScript Target Protectors
		Selected qRT-PCR Assays for microRNAs that regulate NET1 (see all 23):
		hsa-miR-199b-3p hsa-miR-199a-3p hsa-miR-657 hsa-miR-3129-5p hsa-miR-132 hsa-miR-340 hsa-miR-144 hsa-miR-548x

		SwitchGear 3'UTR luciferase reporter plasmid: NET1 3' UTR sequence

Inhib. RNA Products:		OriGene RNAi products in human, mouse, rat for NET1
		Predesigned siRNA for gene silencing in human, mouse, rat NET1

Clone Products:		OriGene clones in human, mouse for NET1 (see all 12) OriGene ORF clones in mouse, rat for NET1 OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
		GenScript: all cDNA clones in your preferred vector (see all 2): NET1 (NM_005863)
		DNA2.0 Custom Codon Optimized Gene Synthesis Service for NET1
		Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat NET1

Primer Products:		OriGene qPCR primer pairs and template standards for NET1 OriGene qSTAR qPCR primer pairs in human, mouse for NET1
		Pre-validated RT² qPCR Primer Assay in human, mouse, rat NET1
		QuantiTect SYBR Green Assays in human, mouse, rat NET1
		QuantiFast Probe-based Assays in human, mouse, rat NET1

Additional mRNA sequence:

AB209852.1 AJ010046.1 AK024919.1 AK294244.1 AK299335.1 AK302687.1 AK304028.1 AK315566.1

BC010285.1 BC053553.1 NR_073040.1 S82401.1 U02081.1

18 DOTS entries:

DT.97860210 DT.455207 DT.86838054 DT.99991536 DT.100792472 DT.455206 DT.97810157 DT.121221194

DT.102826903 DT.121221244 DT.95301494 DT.40279022 DT.92447449 DT.121221239 DT.121221261 DT.447089

DT.92384021 DT.97860209

Selected AceView cDNA sequences (see all 386):

BM767301 AW631462 BQ024362 AI886132 BP376957 BG675849 AU280423 AW087613

AA025841 CA394283 BQ024592 BM771515 BQ181013 CB119951 CA489179 AA831287

AI610995 BM835259 BC053553 BM783408 BQ925376 CB149941 BG493138 AA062769

GeneLoc Exon Structure

Selected Alternative Splicing Database (ASD) splice patterns (SP) for NET1 (see all 10) About this scheme

ExUns:	1a	·	1b	·	1c	^	2	^	3	^	4	^	5	^	6a	·	6b	·	6c	^	7a	·	7b	^	8a	·	8b	·	8c	^	9a	·	9b	^	10	^	11a	·	11b	^	12	^	13a	·	13b	^	14
SP1:											-		-						-				-		-																				-
SP2:																			-				-		-																				-
SP3:											-		-						-				-
SP4:													-
SP5:																																													-

ECgene alternative splicing isoforms for NET1

(RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE ¹MOPED, ²PaxDb, and ³MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
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NET1 expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS <intensity>^2/3
CGAP TAG: AAATGGCTTG

About this image

NET1 expression in embryonic tissues and stem cells About this table

Data from LifeMap, the Embryonic Development and Stem Cells Database
selected tissues (see all 6) fully expand


Limb (Muscoskeletal System)
Limb Bud Mesenchyme Cells Limb Bud

Mesoderm (Gastrulation Derivatives)
Limb Bud Mesenchyme Cells Limb Bud

Colon (Gastrointestinal Tract)

Esophagus (Gastrointestinal Tract)

Placenta (Extraembryonic Tissues)

NET1 Protein expression data from MOPED¹, PaxDb² and MaxQB³:

About this image

NET1 Protein Expression

SOURCE GeneReport for Unigene cluster: Hs.25155

UniProtKB/Swiss-Prot: ARHG8_HUMAN, Q7Z628

Tissue specificity: Widely expressed

Custom PCR Arrays for NET1

Primer Products:		OriGene qPCR primer pairs and template standards for NET1 OriGene qSTAR qPCR primer pairs in human, mouse for NET1
		Pre-validated RT² qPCR Primer Assay in human, mouse, rat NET1
		QuantiTect SYBR Green Assays in human, mouse, rat NET1
		QuantiFast Probe-based Assays in human, mouse, rat NET1

In Situ
Assay Products:

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for NET1

(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD , ⁵MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or ⁶Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
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This gene was present in the common ancestor of animals and fungi.

Orthologs for NET1 gene from Selected species (see all 15) About this table

Organism	Taxonomic classification	Gene	Description	Human Similarity	Orthology Type	Details
mouse (Mus musculus)	Mammalia	Net1^{1 , 5}	neuroepithelial cell transforming gene 1^{1, 5}	85.32(n)¹ 87.06(a)¹		13 (2.17 cM)⁵ 56349¹ NM_019671.3¹ NP_062645.2¹ 3882565⁵
chicken (Gallus gallus)	Aves	NET1¹	neuroepithelial cell transforming 1	76.6(n) 80.78(a)		416693 NM_001030648.1 NP_001025819.1
lizard (Anolis carolinensis)	Reptilia	NET1⁶	neuroepithelial cell transforming 1	74(a)	1 ↔ 1	GL343728.1(88759-176566)
African clawed frog (Xenopus laevis)	Amphibia	BC042313.1²	--	78.49(n)		BC042313.1
zebrafish (Danio rerio)	Actinopterygii	net1¹	neuroepithelial cell transforming gene 1	64.94(n) 70.92(a)		493607 NM_001007767.1 NP_001007768.1
fruit fly (Drosophila melanogaster)	Insecta	Gef64C⁶	Guanine nucleotide exchange factor GEF64C	4(a)	many ↔ many	3L(4692783-4796253)
baker's yeast (Saccharomyces cerevisiae)	Saccharomycetes	ROM1(YGR070W)⁴	GDP/GTP exchange protein (GEP) for Rho1p; mutations more	--		7(627806-631273) 852961 NP_011584.1

ENSEMBL Gene Tree for NET1 (if available)
TreeFam Gene Tree for NET1 (if available)

(Paralogs according to ¹HomoloGene,
²Ensembl, and ³SIMAP, Pseudogenes according to ⁴Pseudogene.org Build 68)
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Paralogs for NET1 gene

ARHGEF2² ARHGEF3² ARHGEF11² ARHGEF1² ENSG00000268861² ARHGEF12² AKAP13² ARHGEF18²

ARHGEF28²

8 SIMAP similar genes for NET1 using alignment to 5 protein entries: ARHG8_HUMAN (see all proteins):

ARHGEF3 BCR/ABL fusion PREX1 ITSN1 PREX2 ARHGEF12

ARHGEF1 ITSN2

NET1 for paralogs About GeneDecksing

3 Pseudogenes.org Pseudogenes for NET1
PGOHUM00000235063 PGOHUM00000245126 PGOHUM00000233408

(SNPs/Variants according to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, ⁴UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
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SNP ID	Valid	Clinical significance	Chr 10 pos	Sequence	#	AA Chg	Type	More	#	Allele freq	Pop	Total sample	More
Genomic Data					Transcription Related Data				Allele Frequencies
				--	--	--		--	--
VAR_035972⁴		A breast cancer sample⁴	--	see VAR_035972	2	D N	mis⁴		0	--	--	--	--
rs11463471^1,2	C	--	5452533(+)	`ACTCT-/TAACTG`	1	--	us2k¹		0	--	--	--	--
rs139034646^1,2		--	5452584(+)	`ATGGGA/GAAGGT`	1	--	us2k¹		0	--	--	--	--
rs182992019^1,2		--	5452644(+)	`CCTTCC/TGGTGG`	1	--	us2k¹		0	--	--	--	--
rs187357396^1,2		--	5452743(+)	`GAGCAG/TCAGCA`	1	--	us2k¹		0	--	--	--	--
rs60713184^1,2	C,F	--	5452758(+)	`TTATTG/ATAAAG`	1	--	us2k¹		3		WA	122
rs191050513^1,2		--	5452793(+)	`GTGTGA/GAAGGG`	1	--	us2k¹		0	--	--	--	--
rs75829724^1,2	C	--	5452799(+)	`CAGGGT/GACCCG`	1	--	us2k¹		1		NA	2
rs184093748^1,2		--	5452898(+)	`TTTTTA/TCAATC`	1	--	us2k¹		0	--	--	--	--
rs188596056^1,2		--	5453094(+)	`TCAATG/TTTACT`	1	--	us2k¹		0	--	--	--	--

HapMap Linkage Disequilibrium report for NET1 (5454514 - 5500426 bp)

Structural Variations
Database of Genomic Variants (DGV) 5 variations for NET1: About this table

Variant ID	Type	Subtype	PubMed ID
esv2731718	CNV	Deletion	23290073
nsv894783	CNV	Loss	21882294
dgv600n71	CNV	Loss	21882294
nsv831774	CNV	Gain	17160897
nsv894792	CNV	Gain	21882294

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(in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Conferences by KenesGroup, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
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OMIM gene information: 606450 OMIM disorders: --

17 diseases for NET1:
About MalaCards

skin squamous cell carcinoma attention deficit hyperactivity disorder breast and colorectal cancer herpes simplex

hepatocellular carcinoma alzheimer's disease squamous cell carcinoma retinitis

breast cancer adenocarcinoma pancreatitis colorectal cancer

leukemia

NET1 for disorders About GeneDecksing

Congresses - knowledge worth sharing:
Alzheimer's & Parkinson's Diseases Congress (ADPD) 18 - 22 March 2015

1 Novoseek inferred disease relationship for NET1 gene About this table

Disease	-log (P-Val)	Hits	PubMed IDs for Articles with Shared Sentences (# sentences)
neuroepithelioma	87.5	3	15611121 (1), 19124484 (1), 8649828 (1)

Genetic Association Database (GAD): NET1
Human Genome Epidemiology (HuGE) Navigator: NET1 (97 documents)

Export disorders for NET1 gene to outside databases

(in PubMed. Associations of this gene to articles via ¹Entrez Gene, ²UniProtKB/Swiss-Prot, ³HGNC, ⁴GAD, ⁵PharmGKB, ⁶HMDB, ⁷DrugBank, ⁸UniProtKB/TrEMBL, ⁹ Novoseek, and/or ¹⁰fRNAdb)
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PubMed articles for NET1 gene, integrated from 10 sources (see all 53):
(articles sorted by number of sources associating them with NET1)

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world of online information

Isolation of a novel oncogene, NET1, from neuroepithelioma cells by expression cDNA cloning. (PubMed id 8649828)^{1, 2, 3, 9} Chan A.M.-L.... Miki T. (Oncogene 1996)
The nuclear guanine nucleotide exchange factors Ect2 and Net1 regulate RhoB-mediated cell death after DNA damage. (PubMed id 21373644)^{1, 2} Srougi M.C. and Burridge K. (PLoS ONE 2011)
Lack of influence of COMT and NET genes variants on executive functions in schizophrenic and bipolar patients, their first-degree relatives and controls. (PubMed id 16741933)^{1, 4} SzAPke A....Leboyer M. (Am. J. Med. Genet. B Neuropsychiatr. Genet. 2006)
A scan of chromosome 10 identifies a novel locus showing strong association with late-onset Alzheimer disease. (PubMed id 16385451)^{1, 4} Grupe A....Goate A. (Am. J. Hum. Genet. 2006)
Support for association between ADHD and two candidate genes: NET1 and DRD1. (PubMed id 15717291)^{1, 4} Bobb A.J....Rapoport J.L. (Am. J. Med. Genet. B Neuropsychiatr. Genet. 2005)
DNA pooling analysis of 21 norepinephrine transporter gene SNPs with attention deficit hyperactivity disorder: no evidence for association. (PubMed id 15719398)^{1, 4} Xu X....Asherson P. (Am. J. Med. Genet. B Neuropsychiatr. Genet. 2005)
The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)^{1, 2} Gerhard D.S.... Malek J. (Genome Res. 2004)
The activity of guanine exchange factor NET1 is essential for transforming growth factor-beta-mediated stress fiber formation. (PubMed id 11278519)^{1, 2} Shen X.... Wang X.-F. (J. Biol. Chem. 2001)
Interaction of the RhoA exchange factor Net1 with discs large homolog 1 protects it from proteasome-mediated degradation and potentiates Net1 activity. (PubMed id 19586902)^{1, 9} Carr H.S....Frost J.A. (J. Biol. Chem. 2009)
Expression and clinical significance of NET-1 and PCNA in hepatocellular carcinoma. (PubMed id 18214716)^{1, 9} Shen S.Q....Nakao A. (Med. Oncol. 2008)

(in PubMed, OMIM, and NCBI Bookshelf)
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		AND	OR
Aliases
Disorders
Free Text

Query String		PubMed OMIM NCBI Bookshelf
	(Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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Entrez Gene: 10276	HGNC: 14592	AceView: NET1	Ensembl:ENSG00000173848	euGenes: HUgn10276
ECgene: NET1	H-InvDB: NET1

(According to HUGE)
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(According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
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Name	Description
PharmGKB entry for NET1	Pharmacogenomics, SNPs, Pathways
ATLAS Chromosomes in Cancer entry for NET1	Genetics and Cytogenetics in Oncology and Haematology

Intellectual Property for NET1 gene
(Patent information from GeneIP, Licensable technologies from WIS Yeda, Salk, Tufts, IP news from LifeMap Sciences, Inc.)

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Patent Information for NET1 gene:
Search GeneIP for patents involving NET1

GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search

(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript, and ESI BIO, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
Advanced Cell Diagnostics, Animal models from inGenious Targeting Laboratory, genOway)
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Neuroepithelial Cell Transforming 1

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Neuroepithelial Cell Transforming 1

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