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Aliases for NEMF Gene

Aliases for NEMF Gene

  • Nuclear Export Mediator Factor 2 3 5
  • Serologically Defined Colon Cancer Antigen 1 2 3 4
  • Antigen NY-CO-1 3 4
  • SDCCAG1 3 4
  • Nuclear Export Mediator Factor NEMF 3
  • NY-CO-1 3

External Ids for NEMF Gene

Previous HGNC Symbols for NEMF Gene

  • SDCCAG1

Previous GeneCards Identifiers for NEMF Gene

  • GC14M050250

Summaries for NEMF Gene

Entrez Gene Summary for NEMF Gene

  • This gene encodes a component of the ribosome quality control complex. The encoded protein facilitates the recognition and ubiquitination of stalled 60S subunits by the ubiquitin ligase listerin. A similar protein in fly functions as a tumor suppressor. [provided by RefSeq, Jul 2016]

GeneCards Summary for NEMF Gene

NEMF (Nuclear Export Mediator Factor) is a Protein Coding gene.

UniProtKB/Swiss-Prot for NEMF Gene

  • Component of the ribosome quality control complex (RQC), a ribosome-associated complex that mediates ubiquitination and extraction of incompletely synthesized nascent chains for proteasomal degradation. NEMF is responsible for selective recognition of stalled 60S subunits by recognizing an exposed, nascent chain-conjugated tRNA moiety. NEMF is important for the stable association of LTN1 to the complex (PubMed:25578875). May indirectly play a role in nuclear export (PubMed:16103875).

Additional gene information for NEMF Gene

No data available for CIViC summary , Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for NEMF Gene

Genomics for NEMF Gene

Regulatory Elements for NEMF Gene

Enhancers for NEMF Gene
GeneHancer Identifier Enhancer Score Enhancer Sources Gene-Enhancer Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor Binding Sites within enhancer Gene Targets for Enhancer
GH14H049860 1.8 FANTOM5 ENCODE dbSUPER 17.1 -9.1 -9092 4 HDGF PKNOX1 FOXA2 MLX ARNT ARID4B SIN3A FEZF1 DMAP1 ZBTB7B KLHDC1 VCPKMT MAP4K5 KLHDC2 NEMF LRR1 POLE2 LINC01588 RHOQP1 RPS29
GH14H049618 1.7 FANTOM5 ENCODE dbSUPER 11.9 +231.0 231018 6 HDGF PKNOX1 ARID4B SIN3A DMAP1 ZNF2 YY1 ZNF143 ZNF207 FOS RPL36AL LRR1 VCPKMT DNAAF2 NEMF RNU6ATAC30P RHOQP1 KLHDC2 ARF6 RPS29
GH14H049584 1.1 ENCODE 16.7 +267.4 267397 4 HDGF ARID4B SIN3A DMAP1 ZNF2 ZBTB7B YY1 ZNF207 ZNF143 FOS LRR1 RPL36AL POLE2 KLHDC1 KLHDC2 VCPKMT NEMF MAP4K5 RHOQP1 RPS29
GH14H050034 1.5 Ensembl ENCODE dbSUPER 11 -185.1 -185132 7 HDGF PKNOX1 ZNF766 GATA2 ZNF143 FOS ATF7 RUNX3 JUNB REST KLHDC1 ENSG00000244270 NEMF DNAAF2 LINC01588 PDLIM1P1 GC14M050001
GH14H050665 1.3 ENCODE dbSUPER 12.2 -815.1 -815086 5 HNRNPUL1 HDGF PKNOX1 ARID4B SIN3A ZNF2 ZNF143 SMARCB1 ZNF592 KAT8 SAV1 KLHDC2 TMX1 KLHDC1 NEMF FRMD6 POLE2 VCPKMT MAP4K5 CDKL1
- Elite enhancer and/or Elite enhancer-gene association Download GeneHancer data dump

Enhancers around NEMF on UCSC Golden Path with GeneCards custom track

Promoters for NEMF Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters
ENSR00000068047 403 2401 MLX ARNT ARID4B SIN3A DMAP1 ZNF2 ZBTB7B YY1 SLC30A9 ZNF143

Genomic Location for NEMF Gene

Chromosome:
14
Start:
49,782,083 bp from pter
End:
49,853,203 bp from pter
Size:
71,121 bases
Orientation:
Minus strand

Genomic View for NEMF Gene

Genes around NEMF on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
NEMF Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for NEMF Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for NEMF Gene

Proteins for NEMF Gene

  • Protein details for NEMF Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    O60524-NEMF_HUMAN
    Recommended name:
    Nuclear export mediator factor NEMF
    Protein Accession:
    O60524
    Secondary Accessions:
    • A0JLQ3
    • B3KSK1
    • B4DDL3
    • B4DHA9
    • B4E3F3
    • Q32Q66
    • Q8WW70
    • Q9NWG1

    Protein attributes for NEMF Gene

    Size:
    1076 amino acids
    Molecular mass:
    122954 Da
    Quaternary structure:
    • Component of the ribosome quality control complex (RQC), composed of at least the E3 ubiquitin ligase LTN1 and NEMF (PubMed:25578875). The complex probably also contains TCF25 as well as VCP/p97 and its ubiquitin-binding cofactors (By similarity). RQC forms a stable complex with 60S ribosomal subunits (PubMed:25578875). Interacts (via its N-terminus) with XPO1 (By similarity).
    Miscellaneous:
    • Active in normal lung tissue, but is inactive in several lung cancer cell lines.
    SequenceCaution:
    • Sequence=AAC18036.1; Type=Frameshift; Positions=1065; Evidence={ECO:0000305}; Sequence=AAH06001.1; Type=Miscellaneous discrepancy; Note=Contaminating sequence. Potential poly-A sequence.; Evidence={ECO:0000305}; Sequence=AAH20794.2; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305}; Sequence=AAH56687.1; Type=Miscellaneous discrepancy; Note=Contaminating sequence. Potential poly-A sequence.; Evidence={ECO:0000305}; Sequence=AAI07765.1; Type=Erroneous initiation; Note=Translation N-terminally shortened.; Evidence={ECO:0000305}; Sequence=BAG52763.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305}; Sequence=BAG58070.1; Type=Erroneous translation; Note=Wrong choice of frame.; Evidence={ECO:0000305}; Sequence=BAG58070.1; Type=Frameshift; Positions=246, 1025; Evidence={ECO:0000305}; Sequence=BAG58070.1; Type=Miscellaneous discrepancy; Note=Intron retention.; Evidence={ECO:0000305};

    Three dimensional structures from OCA and Proteopedia for NEMF Gene

    Alternative splice isoforms for NEMF Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for NEMF Gene

Post-translational modifications for NEMF Gene

  • Ubiquitination at Lys35
  • Modification sites at PhosphoSitePlus

No data available for DME Specific Peptides for NEMF Gene

Domains & Families for NEMF Gene

Gene Families for NEMF Gene

Human Protein Atlas (HPA):
  • Plasma proteins
  • Predicted intracellular proteins

Protein Domains for NEMF Gene

Suggested Antigen Peptide Sequences for NEMF Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

O60524

UniProtKB/Swiss-Prot:

NEMF_HUMAN :
  • The N-terminal domain contains a nuclear export signal. The C-terminal domain may interact with cargo proteins (By similarity).
  • Belongs to the NEMF family.
Domain:
  • The N-terminal domain contains a nuclear export signal. The C-terminal domain may interact with cargo proteins (By similarity).
Family:
  • Belongs to the NEMF family.
genes like me logo Genes that share domains with NEMF: view

Function for NEMF Gene

Molecular function for NEMF Gene

UniProtKB/Swiss-Prot Function:
Component of the ribosome quality control complex (RQC), a ribosome-associated complex that mediates ubiquitination and extraction of incompletely synthesized nascent chains for proteasomal degradation. NEMF is responsible for selective recognition of stalled 60S subunits by recognizing an exposed, nascent chain-conjugated tRNA moiety. NEMF is important for the stable association of LTN1 to the complex (PubMed:25578875). May indirectly play a role in nuclear export (PubMed:16103875).

Phenotypes From GWAS Catalog for NEMF Gene

genes like me logo Genes that share phenotypes with NEMF: view

Animal Models for NEMF Gene

MGI Knock Outs for NEMF:
  • Nemf tm1b(EUCOMM)Hmgu

Animal Model Products

CRISPR Products

miRNA for NEMF Gene

miRTarBase miRNAs that target NEMF

Inhibitory RNA Products

Clone Products

  • Applied Biological Materials Clones for NEMF
  • Vectors and viruses for ORF, Lenti, Retro, Adenovirus, AAV, and more

No data available for Enzyme Numbers (IUBMB) , Gene Ontology (GO) - Molecular Function , Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for NEMF Gene

Localization for NEMF Gene

Subcellular locations from UniProtKB/Swiss-Prot for NEMF Gene

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for NEMF gene
Compartment Confidence
nucleus 4
cytosol 3
cytoskeleton 1

Subcellular locations from the

Human Protein Atlas (HPA)
  • Cytosol (2)
  • Nuclear bodies (2)
  • Nucleoplasm (2)
See all subcellular structures

Gene Ontology (GO) - Cellular Components for NEMF Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus IEA --
genes like me logo Genes that share ontologies with NEMF: view

Pathways & Interactions for NEMF Gene

No Data Available

Gene Ontology (GO) - Biological Process for NEMF Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0051168 nuclear export IMP 16103875
genes like me logo Genes that share ontologies with NEMF: view

No data available for Pathways by source and SIGNOR curated interactions for NEMF Gene

Drugs & Compounds for NEMF Gene

No Compound Related Data Available

Transcripts for NEMF Gene

Unigene Clusters for NEMF Gene

Nuclear export mediator factor:
Representative Sequences:

CRISPR Products

Inhibitory RNA Products

Clone Products

  • Applied Biological Materials Clones for NEMF
  • Vectors and viruses for ORF, Lenti, Retro, Adenovirus, AAV, and more

Alternative Splicing Database (ASD) splice patterns (SP) for NEMF Gene

ExUns: 1a · 1b ^ 2 ^ 3 ^ 4a · 4b ^ 5 ^ 6 ^ 7 ^ 8 ^ 9 ^ 10 ^ 11 ^ 12a · 12b ^ 13 ^ 14a · 14b ^ 15 ^ 16 ^ 17a · 17b ^ 18 ^ 19 ^ 20 ^ 21 ^
SP1: -
SP2:
SP3:
SP4:
SP5:
SP6:
SP7:
SP8: -
SP9:

ExUns: 22 ^ 23 ^ 24a · 24b ^ 25 ^ 26 ^ 27a · 27b
SP1:
SP2:
SP3:
SP4:
SP5: -
SP6:
SP7:
SP8:
SP9:

Relevant External Links for NEMF Gene

GeneLoc Exon Structure for
NEMF
ECgene alternative splicing isoforms for
NEMF

Expression for NEMF Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and CGAP SAGE for NEMF Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Protein differential expression in normal tissues from HIPED for NEMF Gene

This gene is overexpressed in Lung (32.9) and Breast (14.7).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MaxQB, and MOPED for NEMF Gene



Protein tissue co-expression partners for NEMF Gene

- Elite partner

NURSA nuclear receptor signaling pathways regulating expression of NEMF Gene:

NEMF

SOURCE GeneReport for Unigene cluster for NEMF Gene:

Hs.655964

mRNA Expression by UniProt/SwissProt for NEMF Gene:

O60524-NEMF_HUMAN
Tissue specificity: Expressed in brain, heart, liver, lung, spleen, and skeletal muscle. Also expressed at lower levels in stomach and testis.

Evidence on tissue expression from TISSUES for NEMF Gene

  • Nervous system(4.7)
  • Intestine(4.3)
  • Kidney(4.3)
  • Liver(4.3)
genes like me logo Genes that share expression patterns with NEMF: view

Primer Products

No data available for mRNA differential expression in normal tissues and Phenotype-based relationships between genes and organs from Gene ORGANizer for NEMF Gene

Orthologs for NEMF Gene

This gene was present in the common ancestor of eukaryotes.

Orthologs for NEMF Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia NEMF 33 34
  • 99.54 (n)
dog
(Canis familiaris)
Mammalia NEMF 33 34
  • 93.43 (n)
cow
(Bos Taurus)
Mammalia NEMF 34 33
  • 92.4 (n)
OneToOne
mouse
(Mus musculus)
Mammalia Nemf 33 16 34
  • 88.57 (n)
rat
(Rattus norvegicus)
Mammalia Nemf 33
  • 87.82 (n)
oppossum
(Monodelphis domestica)
Mammalia NEMF 34
  • 81 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia NEMF 34
  • 78 (a)
OneToOne
chicken
(Gallus gallus)
Aves NEMF 34 33
  • 73.92 (n)
OneToOne
lizard
(Anolis carolinensis)
Reptilia -- 34
  • 85 (a)
OneToMany
-- 34
  • 84 (a)
OneToMany
-- 34
  • 57 (a)
OneToMany
tropical clawed frog
(Silurana tropicalis)
Amphibia nemf 33
  • 70.33 (n)
zebrafish
(Danio rerio)
Actinopterygii Dr.10362 33
rainbow trout
(Oncorhynchus mykiss)
Actinopterygii Omy.12972 33
fruit fly
(Drosophila melanogaster)
Insecta Clbn 33 34
  • 57.82 (n)
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP002680 33
  • 51.58 (n)
worm
(Caenorhabditis elegans)
Secernentea Y82E9BR.18 33 34
  • 52.92 (n)
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes TAE2 34
  • 28 (a)
OneToOne
RQC2 36
thale cress
(Arabidopsis thaliana)
eudicotyledons emb1441 33
  • 51.37 (n)
rice
(Oryza sativa)
Liliopsida Os12g0564600 33
  • 50.05 (n)
Os.26814 33
wheat
(Triticum aestivum)
Liliopsida Ta.8214 33
sea squirt
(Ciona savignyi)
Ascidiacea -- 34
  • 50 (a)
OneToOne
fission yeast
(Schizosaccharomyces pombe)
Schizosaccharomycetes SPCC132.01c 33
  • 48.64 (n)
sea squirt
(Ciona intestinalis)
Ascidiacea Cin.8376 33
Species where no ortholog for NEMF was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)

Evolution for NEMF Gene

ENSEMBL:
Gene Tree for NEMF (if available)
TreeFam:
Gene Tree for NEMF (if available)

Paralogs for NEMF Gene

No data available for Paralogs for NEMF Gene

Variants for NEMF Gene

Sequence variations from dbSNP and Humsavar for NEMF Gene

SNP ID Clin Chr 14 pos Sequence Context AA Info Type
rs864622015 Uncertain significance 49,799,508(-) AATCA(-/CA)GAGCC nc-transcript-variant, reference, frameshift-variant
rs1000166763 -- 49,806,409(+) GCGCC(C/T)GCCAC intron-variant
rs1000198373 -- 49,791,030(+) TCTTA(C/G)CAAGT intron-variant
rs1000226567 -- 49,797,564(+) TGGGA(C/G)GCAGA intron-variant
rs1000348701 -- 49,791,144(+) TCCTG(A/G)CCAAC intron-variant

Structural Variations from Database of Genomic Variants (DGV) for NEMF Gene

Variant ID Type Subtype PubMed ID
esv28926 CNV gain+loss 19812545
nsv1138299 CNV deletion 24896259
nsv509533 CNV insertion 20534489

Variation tolerance for NEMF Gene

Residual Variation Intolerance Score: 7.66% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 1.93; 35.93% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for NEMF Gene

Human Gene Mutation Database (HGMD)
NEMF
SNPedia medical, phenotypic, and genealogical associations of SNPs for
NEMF

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for NEMF Gene

Disorders for NEMF Gene

Relevant External Links for NEMF

Atlas of Genetics and Cytogenetics in Oncology and Haematology:
NEMF

No disorders were found for NEMF Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for NEMF Gene

Publications for NEMF Gene

  1. Characterization of human colon cancer antigens recognized by autologous antibodies. (PMID: 9610721) Scanlan MJ … Old LJ (International journal of cancer 1998) 2 3 4 60
  2. Structure and assembly pathway of the ribosome quality control complex. (PMID: 25578875) Shao S … Hegde RS (Molecular cell 2015) 3 4 60
  3. Global, in vivo, and site-specific phosphorylation dynamics in signaling networks. (PMID: 17081983) Olsen JV … Mann M (Cell 2006) 3 4 60
  4. Drosophila caliban, a nuclear export mediator, can function as a tumor suppressor in human lung cancer cells. (PMID: 16103875) Bi X … Mortin MA (Oncogene 2005) 3 4 60
  5. Complete sequencing and characterization of 21,243 full-length human cDNAs. (PMID: 14702039) Ota T … Sugano S (Nature genetics 2004) 3 4 60

Products for NEMF Gene

Sources for NEMF Gene

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