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Aliases for NELFB Gene

Aliases for NELFB Gene

  • Negative Elongation Factor Complex Member B 2 3
  • Cofactor Of BRCA1 2 3 4
  • COBRA1 3 4 6
  • KIAA1182 4 6
  • NELF-B 3 4
  • Negative Elongation Factor Protein B 3
  • Negative Elongation Factor B 3

External Ids for NELFB Gene

Previous Symbols for NELFB Gene

  • COBRA1

Summaries for NELFB Gene

Entrez Gene Summary for NELFB Gene

  • NELFB is a subunit of negative elongation factor (NELF), which also includes NELFA (WHSC2; MIM 606026), either NELFC or NELFD (TH1L; MIM 605297), and NELFE (RDBP; MIM 154040). NELF acts with DRB sensitivity-inducing factor (DSIF), a heterodimer of SPT4 (SUPT4H1; MIM 603555) and SPT5 (SUPT5H; MIM 602102), to cause transcriptional pausing of RNA polymerase II (see MIM 180660) (Narita et al., 2003 [PubMed 12612062]).[supplied by OMIM, Mar 2008]

GeneCards Summary for NELFB Gene

NELFB (Negative Elongation Factor Complex Member B) is a Protein Coding gene. Among its related pathways are Disease and Gene Expression.

UniProtKB/Swiss-Prot for NELFB Gene

  • Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. May be able to induce chromatin unfolding.

Gene Wiki entry for NELFB Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for NELFB Gene

Genomics for NELFB Gene

Genomic Location for NELFB Gene

Start:
137,255,173 bp from pter
End:
137,273,548 bp from pter
Size:
18,376 bases
Orientation:
Plus strand

Genomic View for NELFB Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for NELFB Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for NELFB Gene

No data available for Regulatory Elements for NELFB Gene

Proteins for NELFB Gene

  • Protein details for NELFB Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q8WX92-NELFB_HUMAN
    Recommended name:
    Negative elongation factor B
    Protein Accession:
    Q8WX92
    Secondary Accessions:
    • A2BFA3
    • Q96EW5
    • Q9H9R4
    • Q9ULN8
    • Q9Y3W0

    Protein attributes for NELFB Gene

    Size:
    580 amino acids
    Molecular mass:
    65697 Da
    Quaternary structure:
    • The NELF complex is composed of NELFA, NELFB, NELFCD (isoform NELF-C or isoform NELF-D) and NELFE. Interacts with the first BRCT repeat of BRCA1. Interacts with KIAA1191.
    SequenceCaution:
    • Sequence=AAH11892.1; Type=Erroneous initiation; Evidence={ECO:0000305}; Sequence=BAB14157.1; Type=Erroneous initiation; Evidence={ECO:0000305}; Sequence=CAB43381.3; Type=Erroneous initiation; Evidence={ECO:0000305};

neXtProt entry for NELFB Gene

Proteomics data for NELFB Gene at MOPED

Post-translational modifications for NELFB Gene

  • Modification sites at PhosphoSitePlus
  • Ubiquitination at Lys99 and Lys135

Other Protein References for NELFB Gene

ENSEMBL proteins:
Reactome Protein details:
REFSEQ proteins:

No data available for DME Specific Peptides for NELFB Gene

Domains for NELFB Gene

Protein Domains for NELFB Gene

InterPro:
Blocks:
ProtoNet:

UniProtKB/Swiss-Prot:

NELFB_HUMAN
Family:
  • Belongs to the NELF-B family.:
    • Q8WX92
genes like me logo Genes that share domains with NELFB: view

No data available for Gene Families for NELFB Gene

Function for NELFB Gene

Molecular function for NELFB Gene

UniProtKB/Swiss-Prot Function: Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. May be able to induce chromatin unfolding.

Gene Ontology (GO) - Molecular Function for NELFB Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005515 protein binding IPI 11739404
genes like me logo Genes that share ontologies with NELFB: view

Phenotypes for NELFB Gene

genes like me logo Genes that share phenotypes with NELFB: view

Animal Models for NELFB Gene

MGI Knock Outs for NELFB:

miRNA for NELFB Gene

miRTarBase miRNAs that target NELFB

No data available for Enzyme Numbers (IUBMB) , Transcription Factor Targeting and HOMER Transcription for NELFB Gene

Localization for NELFB Gene

Subcellular locations from UniProtKB/Swiss-Prot for NELFB Gene

Gene Ontology (GO) - Cellular Components for NELFB Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005634 nucleus --
GO:0005654 nucleoplasm TAS --
GO:0005737 cytoplasm IDA --
GO:0032021 NELF complex IDA 12612062
genes like me logo Genes that share ontologies with NELFB: view

Pathways for NELFB Gene

genes like me logo Genes that share pathways with NELFB: view

Pathways by source for NELFB Gene

Gene Ontology (GO) - Biological Process for NELFB Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006366 transcription from RNA polymerase II promoter TAS --
GO:0006368 transcription elongation from RNA polymerase II promoter TAS --
GO:0010467 gene expression TAS --
GO:0016032 viral process TAS --
GO:0045892 negative regulation of transcription, DNA-templated IEA --
genes like me logo Genes that share ontologies with NELFB: view

Transcripts for NELFB Gene

mRNA/cDNA for NELFB Gene

(1) REFSEQ mRNAs :
(7) Additional mRNA sequences :
(64) Selected AceView cDNA sequences:
(1) Ensembl transcripts including schematic representations, and UCSC links where relevant :

Unigene Clusters for NELFB Gene

Negative elongation factor complex member B:
Representative Sequences:

Alternative Splicing Database (ASD) splice patterns (SP) for NELFB Gene

ExUns: 1 ^ 2 ^ 3a · 3b · 3c ^ 4 ^ 5a · 5b · 5c · 5d ^ 6 ^ 7a · 7b ^ 8 ^ 9 ^ 10 ^ 11 ^ 12 ^ 13 ^ 14
SP1: - - - -
SP2: - -
SP3: - -
SP4:
SP5: -
SP6: -

Relevant External Links for NELFB Gene

GeneLoc Exon Structure for
NELFB
ECgene alternative splicing isoforms for
NELFB

Expression for NELFB Gene

mRNA expression in normal human tissues for NELFB Gene

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, MOPED, and MaxQB for NELFB Gene

SOURCE GeneReport for Unigene cluster for NELFB Gene Hs.521809

mRNA Expression by UniProt/SwissProt for NELFB Gene

Q8WX92-NELFB_HUMAN
Tissue specificity: Widely expressed. Expressed in heart, brain, lung, placenta, liver, skeletal muscle, kidney and pancreas.
genes like me logo Genes that share expressions with NELFB: view

Orthologs for NELFB Gene

This gene was present in the common ancestor of animals.

Orthologs for NELFB Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia COBRA1 36
  • 98.3 (n)
  • 98.56 (a)
NELFB 37
  • 100 (a)
OneToOne
cow
(Bos Taurus)
Mammalia COBRA1 37
  • 96 (a)
OneToOne
NELFB 36
  • 89.37 (n)
  • 96.38 (a)
dog
(Canis familiaris)
Mammalia NELFB 36
  • 89.48 (n)
  • 96.21 (a)
NELFB 37
  • 96 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Nelfb 36
  • 85.63 (n)
  • 95.86 (a)
Nelfb 16
Nelfb 37
  • 96 (a)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia NELFB 37
  • 91 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia NELFB 37
  • 84 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Nelfb 36
  • 86.15 (n)
  • 96.55 (a)
chicken
(Gallus gallus)
Aves COBRA1 36
  • 78.26 (n)
  • 87.3 (a)
COBRA1 37
  • 87 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia NELFB 37
  • 86 (a)
OneToOne
African clawed frog
(Xenopus laevis)
Amphibia Xl.7402 36
tropical clawed frog
(Silurana tropicalis)
Amphibia nelfb 36
  • 76.91 (n)
  • 80.45 (a)
zebrafish
(Danio rerio)
Actinopterygii nelfb 36
  • 71.9 (n)
  • 77.31 (a)
nelfb 37
  • 73 (a)
OneToOne
zgc55628 36
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP012109 36
  • 62.76 (n)
  • 54.69 (a)
fruit fly
(Drosophila melanogaster)
Insecta NELF-B 36
  • 59 (n)
  • 54.34 (a)
NELF-B 37
  • 51 (a)
OneToOne
sea squirt
(Ciona intestinalis)
Ascidiacea Cin.3713 36
Species with no ortholog for NELFB:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for NELFB Gene

ENSEMBL:
Gene Tree for NELFB (if available)
TreeFam:
Gene Tree for NELFB (if available)

Paralogs for NELFB Gene

No data available for Paralogs for NELFB Gene

Variants for NELFB Gene

Sequence variations from dbSNP and Humsavar for NELFB Gene

SNP ID Clin Chr 09 pos Sequence Context AA Info Type MAF
rs8281 -- 137,273,425(+) CCCAG(A/C/G/T)TTGCA utr-variant-3-prime
rs1135234 -- 137,271,132(+) AATCA(A/G)GGCAT intron-variant
rs3204123 -- 137,272,570(+) GCCCC(A/C/G/T)TCTAA synonymous-codon, reference
rs3936177 -- 137,267,184(-) TCAGC(C/T)CCACA intron-variant
rs9330192 -- 137,266,882(-) CTGAC(A/G)TGACA intron-variant

Relevant External Links for NELFB Gene

HapMap Linkage Disequilibrium report
NELFB

No data available for Structural Variations from Database of Genomic Variants (DGV) for NELFB Gene

Disorders for NELFB Gene

No disorders were found for NELFB Gene.

No data available for UniProtKB/Swiss-Prot for NELFB Gene

Publications for NELFB Gene

  1. Cofactor of BRCA1 modulates androgen-dependent transcription and alternative splicing. (PMID: 17659869) Sun J. … Li R. (J. Steroid Biochem. Mol. Biol. 2007) 2 3 23
  2. Deregulation of cofactor of BRCA1 expression in breast cancer cells. (PMID: 17910036) Sun J. … Li R. (J. Cell. Biochem. 2008) 2 3 23
  3. Characterization of cDNA clones selected by the GeneMark analysis from size-fractionated cDNA libraries from human brain. (PMID: 10574461) Hirosawa M. … Ohara O. (DNA Res. 1999) 2 3 4
  4. Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs. (PMID: 11230166) Wiemann S. … Poustka A. (Genome Res. 2001) 2 3 4
  5. BRCA1-induced large-scale chromatin unfolding and allele-specific effects of cancer-predisposing mutations. (PMID: 11739404) Ye Q. … Li R. (J. Cell Biol. 2001) 3 4

Products for NELFB Gene

  • DNA Methylation CpG Assay Predesigned for Pyrosequencing in human,mouse,rat
  • QIAGEN qRT-PCR Assays for microRNAs that regulate NELFB
    • QuantiTect SYBR Green Assays in human,mouse,rat
    • Pre-validated RT² qPCR Primer Assay in human,mouse,rat
    • QuantiFast Probe-based Assays in human,mouse,rat
    • Predesigned siRNA for gene silencing in human,mouse,rat for NELFB
    • Block miRNA regulation of NELFB using miScript Target Protectors

    Sources for NELFB Gene

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