Aliases for NELFB Gene
Aliases for NELFB Gene
External Ids for NELFB Gene
- HGNC: 24324
- Entrez Gene: 25920
- Ensembl: ENSG00000188986
- OMIM: 611180
- UniProtKB: Q8WX92
Previous HGNC Symbols for NELFB Gene
- COBRA1
Previous GeneCards Identifiers for NELFB Gene
- GC09P140150
Summaries for NELFB Gene
-
NELFB is a subunit of negative elongation factor (NELF), which also includes NELFA (WHSC2; MIM 606026), either NELFC or NELFD (TH1L; MIM 605297), and NELFE (RDBP; MIM 154040). NELF acts with DRB sensitivity-inducing factor (DSIF), a heterodimer of SPT4 (SUPT4H1; MIM 603555) and SPT5 (SUPT5H; MIM 602102), to cause transcriptional pausing of RNA polymerase II (see MIM 180660) (Narita et al., 2003 [PubMed 12612062]).[supplied by OMIM, Mar 2008]
GeneCards Summary for NELFB Gene
NELFB (Negative Elongation Factor Complex Member B) is a Protein Coding gene. Among its related pathways are Formation of HIV elongation complex in the absence of HIV Tat and Gene Expression.
UniProtKB/Swiss-Prot for NELFB Gene
-
Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II. Binds RNA which may help to stabilize the NELF complex on nucleic acid. In vitro, binds weakly to the HIV-1 TAR RNA which is located in the long terminal repeat (LTR) of HIV-1. May be able to induce chromatin unfolding.
No data available for CIViC summary , Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for NELFB Gene
Genomics for NELFB Gene
GeneHancer (GH) Regulatory Elements for NELFB Gene
Promoters and enhancers for NELFB Gene
- Elite GeneHancer and/or Elite GeneHancer-gene association
Download GeneHancer data dump
GeneHancers around NELFB on UCSC Golden Path with GeneCards custom track
Regulatory Element Products
Genomic Locations for NELFB Gene
Genomic Locations for NELFB Gene
- chr9:137,255,173-137,273,548
- (GRCh38/hg38)
- Size:
- 18,376 bases
- Orientation:
- Plus strand
- chr9:140,149,625-140,168,000
- (GRCh37/hg19)
Genomic View for NELFB Gene
Genes around NELFB on UCSC Golden Path with GeneCards custom track
- Cytogenetic band:
-
- 9q34.3 by Ensembl
- 9q34.3 by Entrez Gene
- 9q34.3 by HGNC
NELFB Gene in genomic location: bands according to
Ensembl, locations according to GeneLoc
(and/or Entrez Gene and/or Ensembl if different)
Genomic Neighborhood
• Exon Structure
• Gene Density
RefSeq DNA sequence for NELFB Gene
Proteins for NELFB Gene
-
Protein details for NELFB Gene (UniProtKB/Swiss-Prot)
- Protein Symbol:
- Q8WX92-NELFB_HUMAN
- Recommended name:
- Negative elongation factor B
- Protein Accession:
- Q8WX92
- A2BFA3
- Q96EW5
- Q9H9R4
- Q9ULN8
- Q9Y3W0
Protein attributes for NELFB Gene
- Size:
- 580 amino acids
- Molecular mass:
- 65697 Da
- Quaternary structure:
-
- The NELF complex is composed of NELFA, NELFB, NELFCD (isoform NELF-C or isoform NELF-D) and NELFE; the N-terminus of NELFB binds to the NELFA:NELFCD subcomplex. Interacts with the first BRCT repeat of BRCA1. Interacts with KIAA1191.
- SequenceCaution:
-
- Sequence=AAH11892.1; Type=Erroneous initiation; Evidence={ECO:0000305}; Sequence=BAB14157.1; Type=Erroneous initiation; Evidence={ECO:0000305}; Sequence=CAB43381.3; Type=Erroneous initiation; Evidence={ECO:0000305};
Protein Expression for NELFB Gene
Post-translational modifications for NELFB Gene
- Ubiquitination at isoforms=99 and Lys135
Other Protein References for NELFB Gene
- ENSEMBL proteins:
- REFSEQ proteins:
Antibody Products
- Cell Signaling Technology (CST) Antibodies for NELFB (COBRA1)
-
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-
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Protein Products
-
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-
Quality Products:
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-
Abcam proteins for NELFB
Assay Products
No data available for DME Specific Peptides for NELFB Gene
Domains & Families for NELFB Gene
Gene Families for NELFB Gene
- Human Protein Atlas (HPA):
-
- Predicted intracellular proteins
Protein Domains for NELFB Gene
- InterPro:
- Blocks:
- ProtoNet:
Suggested Antigen Peptide Sequences for NELFB Gene
- GenScript: Design optimal peptide antigens:
Graphical View of Domain Structure for InterPro Entry
Q8WX92- Family:
-
- Belongs to the NELF-B family.
Function for NELFB Gene
Molecular function for NELFB Gene
- UniProtKB/Swiss-Prot Function:
- Essential component of the NELF complex, a complex that negatively regulates the elongation of transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. The NELF complex is involved in HIV-1 latency possibly involving recruitment of PCF11 to paused RNA polymerase II. Binds RNA which may help to stabilize the NELF complex on nucleic acid. In vitro, binds weakly to the HIV-1 TAR RNA which is located in the long terminal repeat (LTR) of HIV-1. May be able to induce chromatin unfolding.
| GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|---|---|---|
| GO:0003723 | RNA binding | IEA | -- |
| GO:0005515 | protein binding | IPI | 11739404 |
Phenotypes for NELFB Gene
- MGI mutant phenotypes for NELFB:
- inferred from 2 alleles
- GenomeRNAi human phenotypes for NELFB:
Animal Model Products
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-
-
ViGene Biosciences lentiviral particle packaged cDNA for NELFB gene
-
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-
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-
Vectors and viruses for KO, Activation, Repression, and more
-
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miRNA for NELFB Gene
- miRTarBase miRNAs that target NELFB
miRNA Products
- Search ViGene Biosciences for NELFB
Inhibitory RNA Products
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Clone Products
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-
VectorBuilder Other custom vectors
- Mammalian expression: PiggyBac
- Mammalian Tet-on expression: plasmid
- Mammalian conditional (Cre-Lox): plasmid and PiggyBac
- Mammalian shRNA knockdown: lentiviral, adenoviral, AAV, and PiggyBac
- CRISPR: plasmid gRNA, lentiviral gRNA, and donor plasmid
- Bacterial expression: pET, pBAD, and pCS
- Yeast expression
- Applied Biological Materials Clones for NELFB
-
Vectors and viruses for ORF, Lenti, Retro, Adenovirus, AAV, and more
Cell Line Products
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No data available for Enzyme Numbers (IUBMB) , Phenotypes From GWAS Catalog , Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for NELFB Gene
Localization for NELFB Gene
Subcellular locations from UniProtKB/Swiss-Prot for NELFB Gene
- Nucleus.
| Compartment | Confidence |
|---|---|
| nucleus | 5 |
| cytosol | 2 |
- Nucleoplasm (4)
| GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|---|---|---|
| GO:0005634 | nucleus | IEA | -- |
| GO:0005654 | nucleoplasm | TAS | -- |
| GO:0005737 | cytoplasm | IDA | -- |
| GO:0032021 | NELF complex | IDA | 12612062 |
Pathways & Interactions for NELFB Gene
| SuperPathway | Contained pathways | ||
|---|---|---|---|
| 1 | Formation of HIV elongation complex in the absence of HIV Tat | ||
| 2 | HIV Life Cycle |
.92
.65
|
.45
|
| 3 | mRNA Capping | ||
| 4 | Gene Expression |
.48
|
|
| 5 | Chromatin Regulation / Acetylation | ||
Pathways by source for NELFB Gene
1 Cell Signaling Technology pathway for NELFB Gene
1 BioSystems pathway for NELFB Gene
Interacting Proteins for NELFB Gene
Selected Interacting proteins:
Q8WX92-NELFB_HUMAN
ENSP00000339495
for NELFB Gene via
IID
STRING
MINT
UniProtKB
| GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|---|---|---|
| GO:0006351 | transcription, DNA-templated | IEA | -- |
| GO:0006355 | regulation of transcription, DNA-templated | IEA | -- |
| GO:0006366 | transcription by RNA polymerase II | TAS | -- |
| GO:0006368 | transcription elongation from RNA polymerase II promoter | TAS | -- |
| GO:0034244 | negative regulation of transcription elongation from RNA polymerase II promoter | IBA | -- |
No data available for SIGNOR curated interactions for NELFB Gene
Transcripts for NELFB Gene
mRNA/cDNA for NELFB Gene
- (1) REFSEQ mRNAs :
- (7) Additional mRNA sequences :
- (64) Selected AceView cDNA sequences:
- (2) Ensembl transcripts including schematic representations, and UCSC links where relevant :
Unigene Clusters for NELFB Gene
CRISPR Products
-
OriGene CRISPR knockouts for NELFB
- genomics-online: gRNA clones - Search results for 90 available COBRA1 gene related products
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-
Vectors and viruses for KO, Activation, Repression, and more
-
Santa Cruz Biotechnology (SCBT) CRISPR for NELFB
- GenScript: Design CRISPR guide RNA sequences for NELFB
miRNA Products
- Search ViGene Biosciences for NELFB
Inhibitory RNA Products
- Origene RNAi, shrna, and sirna products in human, mouse, rat for NELFB
- Browse OriGene Inhibitory RNA Products For NELFB
- genomics-online: shRNA clones - Search results for 90 available COBRA1 gene related products
- Overview of 90 available COBRA1 gene related products
-
ViGene Biosciences ready-to-package AAV shRNAs for NELFB gene
Clone Products
- Sino Biological Human cDNA Clone for NELFB
- Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat
- VectorBuilder custom plasmid, inducible vectors for NELFB
- VectorBuilder custom lentivirus, adenovirus, AAV vector/virus packaging for NELFB
-
VectorBuilder Other custom vectors
- Mammalian expression: PiggyBac
- Mammalian Tet-on expression: plasmid
- Mammalian conditional (Cre-Lox): plasmid and PiggyBac
- Mammalian shRNA knockdown: lentiviral, adenoviral, AAV, and PiggyBac
- CRISPR: plasmid gRNA, lentiviral gRNA, and donor plasmid
- Bacterial expression: pET, pBAD, and pCS
- Yeast expression
- Applied Biological Materials Clones for NELFB
-
Vectors and viruses for ORF, Lenti, Retro, Adenovirus, AAV, and more
| ExUns: | 1 | ^ | 2 | ^ | 3a | · | 3b | · | 3c | ^ | 4 | ^ | 5a | · | 5b | · | 5c | · | 5d | ^ | 6 | ^ | 7a | · | 7b | ^ | 8 | ^ | 9 | ^ | 10 | ^ | 11 | ^ | 12 | ^ | 13 | ^ | 14 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| SP1: | - | - | - | - | |||||||||||||||||||||||||||||||||||
| SP2: | - | - | |||||||||||||||||||||||||||||||||||||
| SP3: | - | - | |||||||||||||||||||||||||||||||||||||
| SP4: | |||||||||||||||||||||||||||||||||||||||
| SP5: | - | ||||||||||||||||||||||||||||||||||||||
| SP6: | - |
Expression for NELFB Gene
This gene is overexpressed in Bone (19.1) and Breast (15.5).
Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MaxQB, and MOPED for NELFB Gene
- Elite partner
NURSA nuclear receptor signaling pathways regulating expression of NELFB Gene:
NELFBSOURCE GeneReport for Unigene cluster for NELFB Gene:
Hs.521809mRNA Expression by UniProt/SwissProt for NELFB Gene:
Q8WX92-NELFB_HUMAN
Tissue specificity:
Widely expressed. Expressed in heart, brain, lung, placenta, liver, skeletal muscle, kidney and pancreas.
Evidence on tissue expression from TISSUES for NELFB Gene
- Nervous system(4.8)
- Liver(4.2)
- Lung(4.2)
Primer Products
-
OriGene qPCR primer pairs for NELFB
-
OriGene qPCR primer pairs and template standards for NELFB
- genomics-online: primer clones - Search results for 90 available COBRA1 gene related products
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No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery , mRNA differential expression in normal tissues and Phenotype-based relationships between genes and organs from Gene ORGANizer for NELFB Gene
Orthologs for NELFB Gene
This gene was present in the common ancestor of animals.
| Organism | Taxonomy | Gene | Similarity | Type | Details |
|---|---|---|---|---|---|
| chimpanzee (Pan troglodytes) |
Mammalia | NELFB 34 |
|
OneToOne | |
| COBRA1 33 |
|
||||
| cow (Bos Taurus) |
Mammalia | COBRA1 34 |
|
OneToOne | |
| NELFB 33 |
|
||||
| oppossum (Monodelphis domestica) |
Mammalia | NELFB 34 |
|
OneToOne | |
| dog (Canis familiaris) |
Mammalia | NELFB 33 34 |
|
||
| rat (Rattus norvegicus) |
Mammalia | Nelfb 33 |
|
||
| mouse (Mus musculus) |
Mammalia | Nelfb 33 16 34 |
|
||
| platypus (Ornithorhynchus anatinus) |
Mammalia | NELFB 34 |
|
OneToOne | |
| chicken (Gallus gallus) |
Aves | COBRA1 33 34 |
|
||
| lizard (Anolis carolinensis) |
Reptilia | NELFB 34 |
|
OneToOne | |
| tropical clawed frog (Silurana tropicalis) |
Amphibia | nelfb 33 |
|
||
| African clawed frog (Xenopus laevis) |
Amphibia | Xl.7402 33 |
|
||
| zebrafish (Danio rerio) |
Actinopterygii | nelfb 33 34 |
|
||
| zgc55628 33 |
|
||||
| African malaria mosquito (Anopheles gambiae) |
Insecta | AgaP_AGAP012109 33 |
|
||
| fruit fly (Drosophila melanogaster) |
Insecta | NELF-B 33 34 |
|
||
| sea squirt (Ciona intestinalis) |
Ascidiacea | Cin.3713 33 |
|
- Species where no ortholog for NELFB was found in the sources mined by GeneCards:
-
- A. gosspyii yeast (Ashbya gossypii)
- Actinobacteria (Mycobacterium tuberculosis)
- Alicante grape (Vitis vinifera)
- alpha proteobacteria (Wolbachia pipientis)
- amoeba (Dictyostelium discoideum)
- Archea (Pyrococcus horikoshii)
- baker's yeast (Saccharomyces cerevisiae)
- barley (Hordeum vulgare)
- beta proteobacteria (Neisseria meningitidis)
- bread mold (Neurospora crassa)
- Chromalveolata (Phytophthora infestans)
- common water flea (Daphnia pulex)
- corn (Zea mays)
- E. coli (Escherichia coli)
- filamentous fungi (Aspergillus nidulans)
- Firmicute bacteria (Streptococcus pneumoniae)
- fission yeast (Schizosaccharomyces pombe)
- green algae (Chlamydomonas reinhardtii)
- honey bee (Apis mellifera)
- K. lactis yeast (Kluyveromyces lactis)
- loblloly pine (Pinus taeda)
- malaria parasite (Plasmodium falciparum)
- medicago trunc (Medicago Truncatula)
- moss (Physcomitrella patens)
- orangutan (Pongo pygmaeus)
- pig (Sus scrofa)
- rainbow trout (Oncorhynchus mykiss)
- rice (Oryza sativa)
- rice blast fungus (Magnaporthe grisea)
- schistosome parasite (Schistosoma mansoni)
- sea anemone (Nematostella vectensis)
- sea urchin (Strongylocentrotus purpuratus)
- sorghum (Sorghum bicolor)
- soybean (Glycine max)
- stem rust fungus (Puccinia graminis)
- sugarcane (Saccharum officinarum)
- thale cress (Arabidopsis thaliana)
- tomato (Lycopersicon esculentum)
- toxoplasmosis (Toxoplasma gondii)
- Trichoplax (Trichoplax adhaerens)
- wheat (Triticum aestivum)
- worm (Caenorhabditis elegans)
Paralogs for NELFB Gene
No data available for Paralogs for NELFB Gene
Variants for NELFB Gene
| SNP ID | Clin | Chr 09 pos | Variation | AA Info | Type |
|---|---|---|---|---|---|
| rs1000113326 | -- | 137,268,223(+) | T/C | intron_variant | |
| rs1000226346 | -- | 137,263,908(+) | C/T | intron_variant | |
| rs1000287829 | -- | 137,262,902(+) | G/A | intron_variant | |
| rs1000305288 | -- | 137,266,890(+) | T/G | intron_variant | |
| rs1000378331 | -- | 137,273,157(+) | C/T | 3_prime_UTR_variant |
| Variant ID | Type | Subtype | PubMed ID |
|---|---|---|---|
| dgv13009n54 | CNV | loss | 21841781 |
| dgv13011n54 | CNV | loss | 21841781 |
| esv2422220 | CNV | deletion | 17116639 |
| esv2739418 | CNV | deletion | 23290073 |
| esv2739419 | CNV | deletion | 23290073 |
| nsv1161918 | CNV | duplication | 26073780 |
| nsv524322 | CNV | loss | 19592680 |
| nsv951207 | CNV | deletion | 24416366 |
Residual Variation Intolerance Score:
7.39% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score:
1.50;
29.25% of all genes are more intolerant (likely to be disease-causing)
Additional Variant Information for NELFB Gene
- SNPedia medical, phenotypic, and genealogical associations of SNPs for
- NELFB
No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for NELFB Gene
Disorders for NELFB Gene
Additional Disease Information for NELFB
No disorders were found for NELFB Gene.
No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for NELFB Gene
Publications for NELFB Gene
- Deregulation of cofactor of BRCA1 expression in breast cancer cells. (PMID: 17910036) Sun J … Li R (Journal of cellular biochemistry 2008) 2 3 22 58
- Cofactor of BRCA1 modulates androgen-dependent transcription and alternative splicing. (PMID: 17659869) Sun J … Li R (The Journal of steroid biochemistry and molecular biology 2007) 2 3 22 58
- Toward a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs. (PMID: 11230166) Wiemann S … Poustka A (Genome research 2001) 2 3 4 58
- Characterization of cDNA clones selected by the GeneMark analysis from size-fractionated cDNA libraries from human brain. (PMID: 10574461) Hirosawa M … Ohara O (DNA research : an international journal for rapid publication of reports on genes and genomes 1999) 2 3 4 58
- Architecture and RNA binding of the human negative elongation factor. (PMID: 27282391) Vos SM … Cramer P (eLife 2016) 3 4 58
Products for NELFB Gene
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- VectorBuilder custom lentivirus, adenovirus, AAV vector/virus packaging for NELFB
- VectorBuilder Other custom vectors
- Mammalian expression: PiggyBac
- Mammalian Tet-on expression: plasmid
- Mammalian conditional (Cre-Lox): plasmid and PiggyBac
- Mammalian shRNA knockdown: lentiviral, adenoviral, AAV, and PiggyBac
- CRISPR: plasmid gRNA, lentiviral gRNA, and donor plasmid
- Bacterial expression: pET, pBAD, and pCS
- Yeast expression
- antibodies-online: Search results for 60 available COBRA1 Antibodies ranked by validation data
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Sources for NELFB Gene
- (1) GeneCards
- (2) HGNC
- (3) EntrezGene
- (4) Swiss-Prot
- (5) Ensembl
- (6) OMIM
- (7) GeneLoc
- (8) Gene Wiki
- (9) UCSC
- (10) PhosphoSitePlus
- (11) GO
- (12) TrEMBL
- (13) InterPro
- (14) ProtoNet
- (15) Blocks
- (16) MGI
- (17) IUBMB
- (18) KEGG
- (19) MINT
- (20) STRING
- (21) IntAct
- (22) Novoseek
- (23) PharmGKB
- (24) DrugBank
- (25) HMDB
- (26) UniGene
- (27) AceView
- (28) ASD
- (29) ECgene
- (30) GeneAnnot
- (31) CGAP SAGE
- (32) SOURCE
- (33) HomoloGene
- (34) PanEnsembl
- (35) euGenes
- (36) SGD
- (37) FlyBase
- (38) WormBase
- (39) Pseudogene
- (40) DGV
- (41) dbSNP
- (42) GenAtlas
- (43) HGMD
- (44) GAD
- (45) BGMUT
- (46) HuGE
- (47) Atlas
- (48) Cell Signaling Technology
- (49) GenBank
- (50) H-invDB
- (51) HORDE
- (52) HUGE
- (53) IMGT
- (54) Leiden
- (55) miRBase
- (56) DME
- (57) OriGene
- (58) PubMed
- (59) R&D Systems
- (60) TGDB
- (61) Tocris
- (62) Abcam
- (63) Novus Biologicals
- (64) ProSpec
- (65) Sino Biological
- (66) GenScript
- (67) Qiagen
- (68) Cloud-Clone Corp.
- (69) OCA
- (70) Proteopedia
- (71) MOPED
- (72) neXtProt
- (73) Reactome
- (74) GeneGo (Thomson Reuters)
- (75) fRNAdb
- (76) DISEASES
- (77) SIMAP
- (78) GenomeRNAi
- (79) LifeMap
- (80) miRTarBase
- (81) MalaCards
- (82) Invitrogen
- (83) BitterDB
- (84) Vector BioLabs
- (85) ESI-BIO
- (86) RefSeq
- (87) BioSystems
- (88) MaxQB
- (89) IUPHAR
- (90) BioGPS
- (91) Illumina
- (92) COMPARTMENTS
- (93) HOMER
- (94) PaxDb
- (95) ApexBio
- (96) Addgene
- (97) antibodies-online
- (98) CYP
- (99) NONCODE
- (100) SwitchGear Genomics
- (101) TreeFam
- (102) PathCards
- (103) GeneReviews
- (104) GeneTex
- (105) Taconic Biosciences
- (106) GTEx
- (107) ProteomicsDB
- (108) SCBT
- (109) DGIdb
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- (128) ViGene Biosciences
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- (132) FANTOM5
- (133) ENCODE
- (134) ProSci
- (135) Horizon
- (136) NURSA
- (137) IID
- (138) Cyagen
- (139) VectorBuilder
- (140) SNPedia
- (141) BRCA Exchange
- (142) St John's Lab
- (143) CIViC
- (144) ProteoGenix
- (145) dbSUPER
- (146) TISSUES
- (147) Gene ORGANizer
- (148) abm
- (149) CrownBio
- (150) Human Protein Atlas
- (151) GWAS Catalog
- (152) Monarch Initiative
- (153) DataMed
- (154) HumanCyc
- (155) genomics-online
- (156) UCNEbase
- (157) EPDnew