Home
GeneCards Guide
Suite
- MalaCards
- GeneALaCart
- GeneDecks
- GeneIP
- GeneLoc
Terms and Conditions
- Terms of Use
- 3rd Party Notice
About Us
- GeneCards Team
- Our Publications
- Citing GeneCards
- Licensing Agreement
- Weizmann Institute
- LifeMap Sciences Inc
User Feedback
Mirror sites

Set Analyses:

Generates a file of GeneCards annotations for your list of genes

Advanced Search

Search By

Section (entire)

for

All GeneCards Within gene subset (to enable gene prioritization):
or upload a file of gene symbols

Category Symbol Source: HGNC EntrezGene Ensembl GeneCards RNA genes CroW21

GIFtS
-

NELFB Gene

protein-coding GIFtS: 49
GCID: GC09P140150

Negative Elongation Factor Complex Member B

(Previous name: cofactor of BRCA1)
(Previous symbol: COBRA1)

Microbiology & Infectious Diseases Congress

See related diseases
at

MalaCards

(According to ¹HGNC, ²Entrez Gene,
³UniProtKB/Swiss-Prot, ⁴UniProtKB/TrEMBL, ⁵OMIM, ⁶GeneLoc, ⁷Ensembl, ⁸DME, ⁹miRBase, ¹⁰fRNAdb, ¹²H-InvDB, ¹³NCBI, ¹⁴NONCODE, and/or ¹⁵RNAdb)
About This Section

Aliases
Negative Elongation Factor Complex Member B¹ ²		KIAA1182³ ⁵
COBRA1¹ ² ³ ⁵		Negative Elongation Factor B²
Cofactor Of BRCA1¹ ² ³		Negative Elongation Factor Protein B²
NELF-B² ³

External Ids:	HGNC: 24324¹	Entrez Gene: 25920²	Ensembl: ENSG00000188986⁷	OMIM: 611180⁵	UniProtKB: Q8WX92³

Export aliases for NELFB gene to outside databases

(According to Entrez Gene, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

Entrez Gene summary for NELFB Gene:

NELFB is a subunit of negative elongation factor (NELF), which also includes NELFA (WHSC2; MIM 606026), either

NELFC or NELFD (TH1L; MIM 605297), and NELFE (RDBP; MIM 154040). NELF acts with DRB sensitivity-inducing factor

(DSIF), a heterodimer of SPT4 (SUPT4H1; MIM 603555) and SPT5 (SUPT5H; MIM 602102), to cause transcriptional

pausing of RNA polymerase II (see MIM 180660) (Narita et al., 2003 (PubMed 12612062)).(supplied by OMIM, Mar

2008)

GeneCards Summary for NELFB Gene:

NELFB (negative elongation factor complex member B) is a protein-coding gene. Diseases associated with NELFB include adenocarcinoma, and breast cancer, and among its related super-pathways are Formation of HIV-1 elongation complex containing HIV-1 Tat and HIV Life Cycle. GO annotations related to this gene include protein binding.

UniProtKB/Swiss-Prot: NELFB_HUMAN, Q8WX92

Function: Essential component of the NELF complex, a complex that negatively regulates the elongation of

transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and

causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. May be able to induce chromatin

unfolding

Gene Wiki entry for NELFB (Cofactor of BRCA1) Gene

(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 73), Regulatory elements and Epigenetics data according to QIAGEN, SABiosciences, and/or SwitchGear Genomics)
About This Section

RefSeq DNA sequence:

NC_000009.11 NC_018920.2 NT_024000.16

Regulatory elements:

Search SABiosciences Regulatory transcription factor binding sites for NELFB
Other transcription factors

SwitchGear Promoter luciferase reporter plasmid: NELFB promoter sequence

Search SABiosciences Chromatin IP Primers for NELFB

Epigenetics:

QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat NELFB

Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 9q34 Ensembl cytogenetic band: 9q34.3 HGNC cytogenetic band: 9q34

NELFB Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
NELFB gene location

GeneLoc information about chromosome 9 GeneLoc Exon Structure

GeneLoc location for GC09P140150: view genomic region (about GC identifiers)

Start:	140,149,625 bp from pter	End:	140,168,000 bp from pter
Size:	18,376 bases	Orientation:	plus strand

(According to UniProtKB, HORDE, neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, Protein expression images according to data from SPIRE ¹MOPED, ²PaxDb, and ³MAXQB RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Cloud-Clone Corp.,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Cloud-Clone Corp., Ontologies according to Gene Ontology Consortium 01 Oct 2013 and Entrez Gene, Antibodies by EMD Millipore, R&D Systems, GenScript, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, and/or Cloud-Clone Corp.)
About This Section

UniProtKB/Swiss-Prot: NELFB_HUMAN, Q8WX92 (See protein sequence)

Recommended Name: Negative elongation factor B

Size: 580 amino acids; 65697 Da

Subunit: The NELF complex is composed of NELFA, NELFB, NELFCD (isoform NELF-C or isoform NELF-D) and NELFE.

Interacts with the first BRCT repeat of BRCA1. Interacts with KIAA1191

Subcellular location: Nucleus

Sequence caution: Sequence=AAH11892.1; Type=Erroneous initiation; Sequence=BAB14157.1; Type=Erroneous initiation;

Sequence=CAB43381.3; Type=Erroneous initiation;

Secondary accessions: A2BFA3 Q96EW5 Q9H9R4 Q9ULN8 Q9Y3W0

Explore the universe of human proteins at neXtProt for NELFB: NX_Q8WX92

Explore proteomics data for NELFB at MOPED

Post-translational modifications:

View modification sites using PhosphoSitePlus

View neXtProt modification sites for NX_Q8WX92

NELFB Protein expression data from MOPED¹, PaxDb² and MAXQB³ : About this image

REFSEQ proteins: NP_056271.2

ENSEMBL proteins:

ENSP00000339495

Reactome Protein details: Q8WX92
Human Recombinant Protein Products for NELFB:

	Browse Purified and Recombinant Proteins at EMD Millipore
	Browse R&D Systems for human recombinant proteins
	Browse recombinant and purified proteins available from Enzo Life Sciences
	Browse OriGene full length recombinant human proteins expressed in human HEK293 cells OriGene Protein Over-expression Lysate for NELFB OriGene Custom MassSpec OriGene Custom Protein Services for NELFB
	GenScript Custom Purified and Recombinant Proteins Services for NELFB
	Novus Biologicals NELFB Protein Novus Biologicals NELFB Lysates
	Browse Sino Biological Recombinant Proteins Browse Sino Biological Cell Lysates
	Browse ProSpec Recombinant Proteins
	Browse Proteins at Cloud-Clone Corp.

Gene Ontology (GO): 5 cellular component terms (GO ID links to tree view): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005634	nucleus	IDA	--
GO:0005654	nucleoplasm	TAS	--
GO:0005730	NOT nucleolus	IDA	--
GO:0005737	cytoplasm	IDA	--
GO:0032021	NELF complex	IDA	12612062

NELFB for ontologies About GeneDecksing

NELFB Antibody Products:

	Browse EMD Millipore's Extensive Line of Mono- and Polyclonal Antibodies
	Browse R&D Systems for Antibodies
	OriGene Antibodies for NELFB OriGene Custom Antibody Services for NELFB
	GenScript Custom Superior Antibodies Services for NELFB
	Novus Biologicals NELFB Antibodies
	Abcam antibodies for NELFB
	Browse Antibodies at Cloud-Clone Corp.
	ThermoFisher Antibodies for NELFB
	Search LSBio for Antibodies for NELFB

Assay Products for NELFB:

	Browse Kits and Assays available from EMD Millipore
	OriGene Custom Assay Services for NELFB
	Browse R&D Systems for biochemical assays
	GenScript Custom Assay Services for NELFB
	Browse Enzo Life Sciences for kits & assays
	Browse ELISAs at Cloud-Clone Corp. Browse CLIAs at Cloud-Clone Corp.

(According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
About This Section

1 InterPro protein domain:

IPR010405 COBRA1

Graphical View of Domain Structure for InterPro Entry Q8WX92

ProtoNet protein and cluster: Q8WX92

1 Blocks protein domain: IPB010405 Cofactor of BRCA1

UniProtKB/Swiss-Prot: NELFB_HUMAN, Q8WX92

Similarity: Belongs to the NELF-B family

NELFB for domains About GeneDecksing

(According to ¹UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or ²DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
bound targets from SABiosciences, miRNA Gene Targets from miRTarBase, shRNA from OriGene, RNAi from EMD Millipore, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, Gene Editing from DNA2.0, Sirion Biotech, Clones from EMD Millipore, OriGene, SwitchGear Genomics, GenScript, Sino Biological, DNA2.0, Vector BioLabs, and Sirion Biotech, Cell Lines from GenScript, LifeMap BioReagents, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Oct 2013 via Entrez Gene.)
About This Section

Molecular Function:

UniProtKB/Swiss-Prot Summary: NELFB_HUMAN, Q8WX92

Function: Essential component of the NELF complex, a complex that negatively regulates the elongation of

transcription by RNA polymerase II. The NELF complex, which acts via an association with the DSIF complex and

causes transcriptional pausing, is counteracted by the P-TEFb kinase complex. May be able to induce chromatin

unfolding

Gene Ontology (GO): 1 molecular function term (GO ID links to tree view): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0005515	protein binding	IPI	11739404

NELFB for ontologies About GeneDecksing

Phenotypes:
2 GenomeRNAi human phenotypes for NELFB:

Decreased nuclei size in G2M

Increased S DNA content, incre

3 MGI mutant phenotypes (inferred from 2 alleles) (MGI details for Nelfb):

embryogenesis

mortality/aging

no phenotypic analysis

NELFB for phenotypes About GeneDecksing

Animal Models:
MGI mouse knock-outs for NELFB: Nelfb^tm1.2Roli Nelfb^tm1.1Roli

inGenious Targeting Laboratory - Custom generated mouse model solutions for NELFB

inGenious Targeting Laboratory - Custom generated inducible mouse model solutions for NELFB

genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for NELFB

genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for NELFB

miRNA Products:		QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat NELFB
		Search QIAGEN for miScript miRNA Assays for microRNAs that regulate NELFB
		SwitchGear 3'UTR luciferase reporter plasmid: NELFB 3' UTR sequence

Inhib. RNA Products:		Browse for Gene Knock-down Tools from EMD Millipore
		OriGene RNAi products in human, mouse, rat for NELFB
		QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat NELFB

Gene Editing Products:			DNA2.0 Custom Protein Engineering Service for NELFB
			Sirion Biotech Customized adenovirus for overexpression of NELFB

Clone Products:		Browse Clones for the Expression of Recombinant Proteins Available from EMD Millipore
		OriGene clones in human, mouse for NELFB (see all 7) OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
		GenScript Custom all cDNA clones Services for NELFB
		Browse Sino Biological Human cDNA Clones
		DNA2.0 Custom Codon Optimized Gene Synthesis Service for NELFB
		Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat NELFB
		Sirion Biotech Customized lentivirus for stable overexpression of NELFB Customized lentivirus expression plasmids for stable overexpression of NELFB

Cell Line Products:		GenScript Custom overexpressing Cell Line Services for NELFB
		Search LifeMap BioReagents cell lines for NELFB

In Situ Assay
Products:

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for NELFB

(Pathways according to EMD Millipore, R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to SABiosciences, and/or STRING, Interactions according to ¹UniProtKB, ²MINT, ³I2D, and/or ⁴STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Oct 2013 via Entrez Gene).
About This Section

SuperPaths for NELFB About

See pathways by source

SuperPath

Selected contained pathways About (see all per SuperPath)

Formation of RNA Pol II elongation complex

HIV Transcription Elongation	0.78	Elongation arrest and recovery	0.74
Formation of HIV-1 elongation complex containing HIV-1 Tat	0.78	Pausing and recovery of HIV elongation	0.74
Tat-mediated elongation of the HIV-1 transcript	0.78	HIV elongation arrest and recovery	0.74
Formation of the HIV-1 Early Elongation Complex	0.78	Pausing and recovery of Tat-mediated HIV elongation	0.73

HIV Life Cycle

	HIV Life Cycle	0.92			HIV Infection	0.61
	Late Phase of HIV Life Cycle	0.92

Transcription

Transcription

0.69

RNA Polymerase II Transcription

0.69

Generic Transcription Pathway

Gene Expression

0.47

Disease

Disease

Pathways by source See SuperPaths
Show all pathways

5/23 Reactome Pathways for NELFB (see all 23)

	Formation of RNA Pol II elongation complex
	Pausing and recovery of Tat-mediated HIV elongation
	Tat-mediated HIV elongation arrest and recovery
	Tat-mediated elongation of the HIV-1 transcript
	Late Phase of HIV Life Cycle

NELFB for pathways About GeneDecksing

Interactions:

Search SABiosciences Gene Network Central^TM Interacting Genes and Proteins Networks for NELFB

STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

5/851 Interacting proteins for NELFB (Q8WX92^{1, 2, 3} ENSP00000339495⁴) via UniProtKB, MINT, STRING, and/or I2D (see all 851)

Interactant		Interaction Details
GeneCard	External ID(s)	Interaction Details
ENSG00000206268	P18615¹^,²^,³	EBI-347721,EBI-348444 MINT-62588 MINT-62762 MINT-62686 I2D: score=5
ENSG00000206357	P18615¹^,²^,³	EBI-347721,EBI-348444 MINT-62588 MINT-62762 MINT-62686 I2D: score=5
ENSG00000229363	P18615¹^,²^,³	EBI-347721,EBI-348444 MINT-62588 MINT-62762 MINT-62686 I2D: score=5
ENSG00000231044	P18615¹^,²^,³	EBI-347721,EBI-348444 MINT-62588 MINT-62762 MINT-62686 I2D: score=5
ENSG00000233801	P18615¹^,²^,³	EBI-347721,EBI-348444 MINT-62588 MINT-62762 MINT-62686 I2D: score=5

About this table

Gene Ontology (GO): 5/6 biological process terms (GO ID links to tree view) (see all 6): About this table

GO ID	Qualified GO term	Evidence	PubMed IDs
GO:0006366	transcription from RNA polymerase II promoter	TAS	--
GO:0006368	transcription elongation from RNA polymerase II promoter	TAS	--
GO:0010467	gene expression	TAS	--
GO:0016032	viral process	TAS	--
GO:0045892	negative regulation of transcription, DNA-dependent	IEA	--

NELFB for ontologies About GeneDecksing

(Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB, with drugs/clinical trials/news search links to CenterWatch)
About This Section

Browse Small Molecules at EMD Millipore

Browse drugs & compounds from Enzo Life Sciences

Browse Tocris compounds for NELFB

Search CenterWatch for drugs/clinical trials and news about NELFB

(Secondary structures according to fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
Conferences by KenesGroup, exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
RNAi Products from EMD Millipore,
siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN,
Tagged/untagged cDNA clones from OriGene, SwitchGear Genomics, GenScript, DNA2.0, Vector BioLabs, Sirion Biotech, Primers from OriGene, SABiosciences, and/or QIAGEN )
About This Section

REFSEQ mRNAs for NELFB gene:

NM_015456.3

Unigene Cluster for NELFB:

Negative elongation factor complex member B

Hs.521809 [show with all ESTs]

Unigene Representative Sequence: NM_015456

1 Ensembl transcript including schematic representation, and UCSC links where relevant:

ENST00000343053(uc004cmm.4)

Congresses - knowledge worth sharing:
European Congress of Clinical Microbiology and Infectious Diseases (ECCMID) 10 - 13 May 2014

miRNA Products:		QIAGEN Custom miScript Target Protector blocks miRNA-binding site of human, mouse, rat NELFB
		Search QIAGEN for miScript miRNA Assays for microRNAs that regulate NELFB
		SwitchGear 3'UTR luciferase reporter plasmid: NELFB 3' UTR sequence

Inhib. RNA Products:		Browse for Gene Knock-down Tools from EMD Millipore
		OriGene RNAi products in human, mouse, rat for NELFB
		QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat NELFB

Clone Products:		OriGene clones in human, mouse for NELFB (see all 7) OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
		GenScript Custom all cDNA clones Services for NELFB
		DNA2.0 Custom Codon Optimized Gene Synthesis Service for NELFB
		Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat NELFB
		Sirion Biotech Customized lentivirus for stable overexpression of NELFB Customized lentivirus expression plasmids for stable overexpression of NELFB

Primer Products:		OriGene qPCR primer pairs and template standards for NELFB OriGene qSTAR qPCR primer pairs in human, mouse for NELFB
		Search SABiosciences RT² qPCR Primer Assays in human, mouse, rat NELFB
		QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat NELFB
		QIAGEN QuantiFast Probe-based Assays in human, mouse, rat NELFB

Additional mRNA sequence:

AB033008.1 AF464935.1 AK022651.1 AK091056.1 AL050280.1 BC011892.1 BC019029.1

19 DOTS entries:

DT.313483 DT.100650419 DT.95345105 DT.100847227 DT.92443894 DT.95345115 DT.95345119 DT.97773518

DT.95345123 DT.99983202 DT.100042649 DT.100650416 DT.100680128 DT.87016426 DT.95244950 DT.95345116

DT.95345117 DT.101984995 DT.121153312

24/64 AceView cDNA sequences (see all 64):

AW958901 BU069420 BM711554 AW134507 CB055237 AA434318 BM665469 BM724872

CK818997 BM126403 BU741500 CK818996 BX379467 BM126112 AI380693 AW674821

AA434230 BM981784 BX354686 AW673957 F36882 BE256079 AL522505 BU069633

GeneLoc Exon Structure

5/6 Alternative Splicing Database (ASD) splice patterns (SP) for NELFB (see all 6) About this scheme

ExUns:	1	^	2	^	3a	·	3b	·	3c	^	4	^	5a	·	5b	·	5c	·	5d	^	6	^	7a	·	7b	^	8	^	9	^	10	^	11	^	12	^	13	^	14
SP1:			-						-				-												-
SP2:			-						-
SP3:									-				-
SP4:
SP5:			-

ECgene alternative splicing isoforms for NELFB

(RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, plus additional links to Genevestigator, and/or SOURCE, and/or BioGPS, and/or UniProtKB,
PCR Arrays from SABiosciences, Primers from OriGene, SABiosciences, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section

NELFB expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS <intensity>^2/3
CGAP TAG: --

About this image

See NELFB Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for NELFB

SOURCE GeneReport for Unigene cluster: Hs.521809

UniProtKB/Swiss-Prot: NELFB_HUMAN, Q8WX92

Tissue specificity: Widely expressed. Expressed in heart, brain, lung, placenta, liver, skeletal muscle, kidney

and pancreas

SABiosciences Custom PCR Arrays for NELFB

Primer Products:		OriGene qPCR primer pairs and template standards for NELFB OriGene qSTAR qPCR primer pairs in human, mouse for NELFB
		Search SABiosciences RT² qPCR Primer Assays in human, mouse, rat NELFB
		QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat NELFB
		QIAGEN QuantiFast Probe-based Assays in human, mouse, rat NELFB

In Situ
Assay Products:

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for NELFB

(Orthologs according to ^1,2HomoloGene (²older version, for species not in ¹newer version), ³euGenes, ⁴SGD , ⁵MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or ⁶Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
About This Section

This gene was present in the common ancestor of animals.

Orthologs for NELFB gene from 6/15 species (see all 15) About this table

Organism	Taxonomic classification	Gene	Description	Human Similarity	Orthology Type	Details
mouse (Mus musculus)	Mammalia	Nelfb⁵ Cobra1¹	cofactor of BRCA1^{1, 5}	85.63(n)¹ 95.86(a)¹		2 (17.06 cM)⁵ 58202¹ NM_021393.2¹ NP_067368.2¹ 25199712⁵
chicken (Gallus gallus)	Aves	COBRA1¹	cofactor of BRCA1	78.26(n) 87.3(a)		417254 NM_001006202.1 NP_001006202.1
lizard (Anolis carolinensis)	Reptilia	COBRA1⁶	Uncharacterized protein	86(a)	1 ↔ 1	GL343625.1(182928-200500)
African clawed frog (Xenopus laevis)	Amphibia	Xl.7402²	Xenopus laevis transcribed sequence with moderate similarity more	79.32(n)		CA790314.1
zebrafish (Danio rerio)	Actinopterygii	zgc55628²	similar to cofactor of BRCA1	75.39(n)		393137 BC045409.1
fruit fly (Drosophila melanogaster)	Insecta	NELF-B¹	CG32721-PA	58.98(n) 54.43(a)		31681 NM_132174.2 NP_572402.1

ENSEMBL Gene Tree for NELFB (if available)
TreeFam Gene Tree for NELFB (if available)

(Paralogs according to ¹HomoloGene,
²Ensembl, and ³SIMAP, Pseudogenes according to ⁴Pseudogene.org Build 68)
About This Section

(SNPs/Variants according to the ¹NCBI SNP Database, ²Ensembl, ³PupaSUITE, ⁴UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD) and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers from QIAGEN, Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section

SNP ID	Valid	Clinical significance	Chr 9 pos	Sequence	#	AA Chg	Type	More	#	Allele freq	Pop	Total sample	More
Genomic Data					Transcription Related Data				Allele Frequencies
				--	--	--		--	--
rs199681335^1,2		--	140150003(+)	`GGCGAA/GGACCT`	2	E	syn¹		0	--	--	--	--
rs201291926^1,2	C	--	140150039(+)	`GAGCCG/TCTCAA`	2	P	syn¹		0	--	--	--	--
rs200780941^1,2	C	--	140150054(+)	`ATCGAA/GCAGTT`	2	E	syn¹		0	--	--	--	--
rs190199686^1,2		--	140150143(+)	`GGCGCC/TGGAAG`	1	--	int¹		0	--	--	--	--
rs78469234^1,2	C,F	--	140150288(+)	`GCCTCC/TCTGTG`	1	--	int¹		1		NA	120
rs11533155^1,2	C,F,H	--	140150310(-)	`GGGCAC/TCCCGA`	1	--	int¹		18		NS EA NA CSA WA EU	2916
rs200129862^1,2		--	140150348(+)	`CTCTTA/GGCTTC`	1	--	int¹		0	--	--	--	--
rs182428492^1,2	C	--	140150353(+)	`GGCTTC/TCTCAG`	1	--	int¹		0	--	--	--	--
rs146836979^1,2	C,F	--	140150413(+)	`TGGACC/TTGCAC`	2	L	syn¹		2		NA EU	5743
rs141153620^1,2	C	--	140150427(+)	`ACGCCG/TCGGCT`	2	P	syn¹		1		NA	4536

HapMap Linkage Disequilibrium report for NELFB (140149625 - 140168000 bp)

Structural Variations
Database of Genomic Variants (DGV) variations for NELFB: --

SABiosciences Cancer Mutation PCR Assays

SeqTarget long-range PCR primers for resequencing NELFB

DNA2.0 Custom Variant and Variant Library Synthesis for NELFB

(in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Conferences by KenesGroup, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
About This Section

OMIM gene information: 611180 OMIM disorders: --

2 diseases for NELFB: About MalaCards

adenocarcinoma breast cancer

NELFB for disorders About GeneDecksing

Export disorders for NELFB gene to outside databases

(in PubMed. Associations of this gene to articles via ¹Entrez Gene, ²UniProtKB/Swiss-Prot, ³HGNC, ⁴GAD, ⁵PharmGKB, ⁶HMDB, ⁷DrugBank, ⁸UniProtKB/TrEMBL, ⁹ Novoseek, and/or ¹⁰fRNAdb)
About This Section

PubMed articles for NELFB gene, integrated from 9 sources (see all 53):
(articles sorted by number of sources associating them with NELFB)

Utopia: connect your pdf to the dynamic
world of online information

Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs. (PubMed id 11230166)^{1, 2, 3} Wiemann S.... Poustka A. (2001)
Characterization of cDNA clones selected by the GeneMark analysis from size-fractionated cDNA libraries from human brain. (PubMed id 10574461)^{1, 2, 3} Hirosawa M.... Ohara O. (1999)
Deregulation of cofactor of BRCA1 expression in breas t cancer cells. (PubMed id 17910036)^{1, 3, 9} Sun J....Li R. (2008)
Cofactor of BRCA1 modulates androgen-dependent transcription and alternative splicing. (PubMed id 17659869)^{1, 3, 9} Sun J....Li R. (2007)
Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)^{1, 2} Ota T.... Sugano S. (2004)
The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)^{1, 2} Gerhard D.S....Malek J. (2004)
Human transcription elongation factor NELF: identification of novel subunits and reconstitution of the functionally active complex. (PubMed id 12612062)^{1, 2} Narita T....Handa H. (2003)
BRCA1-induced large-scale chromatin unfolding and allele-specific effects of cancer-predisposing mutations. (PubMed id 11739404)^{1, 2} Ye Q.... Li R. (2001)
NELF, a multisubunit complex containing RD, cooperates with DSIF to repress RNA polymerase II elongation. (PubMed id 10199401)^{1, 2} Yamaguchi Y.... Handa H. (1999)
Cofactor of BRCA1: a novel transcription factor regulator in upper gastrointestinal adenocarcinomas. (PubMed id 16452188)^{1, 9} McChesney P.A....El-Rifai W. (2006)

(in PubMed, OMIM, and NCBI Bookshelf)
About This Section

		AND	OR
Aliases
Free Text

Query String		PubMed OMIM NCBI Bookshelf
	(Note: In FireFox, select the above section and copy using Ctrl-C)

(According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
About This Section

Entrez Gene: 25920	HGNC: 24324	AceView: COBRA1	Ensembl:ENSG00000188986	euGenes: HUgn25920
ECgene: NELFB	H-InvDB: NELFB

(According to HUGE)
About This Section

HUGE: KIAA1182

(According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section

Name	Description
PharmGKB entry for NELFB	Pharmacogenomics, SNPs, Pathways

Intellectual Property for NELFB gene
(Patent information from GeneIP, Licensable technologies from WIS Yeda, Salk, Tufts, IP news from LifeMap Sciences, Inc.)

About This Section

Patent Information for NELFB gene:
Search GeneIP for patents involving NELFB

GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search

(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, Gene Editing from DNA2.0 and Sirion Biotech, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Sirion Biotech, Cell lines from GenScript, and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or Enzo Life Sciences, In Situ Hybridization Assays from
Advanced Cell Diagnostics, Animal models from inGenious Targeting Laboratory, genOway)
About This Section


	EMD Millipore Custom Antibody & Bulk Services
	EMD Millipore Preclinical / Clinical Development Services
	EMD Millipore Immunoassay Services
	EMD Millipore Target Screening & Profiling Services

	Browse Antibodies		Browse Cell Culture Products
	Browse ELISAs		Browse Flow Cytometry Kits
	Browse Primer Pairs		Browse Kinase Activity Assays/Reagents
	Browse ELISpot Kits/Development Modules		Browse TFB/Immunoprecipitation Assays
	Browse Apoptosis Detection Kits/Reagents		Browse Ubiquitin Proteasome Pathway (UPP) Assay Kits/Reagents
	Browse DNA Damage/Repair Kits/Reagents		Browse Multiplex/Array Assay Kits/Reagents
	Browse Cell Selection/Detection Kits/Reagents		Browse Secondary Antibodies/Controls/Staining Reagents
	Browse Protease Activity Assays and Reagents		Browse Recombinant/Natural Proteins
	Browse Stem Cell Products		Browse Tocris Biochemicals & Compounds
	Browse cDNA Clones		Browse Proteome Profiler Antibody Arrays

	OriGene Antibodies for NELFB		OriGene RNAi products in human, mouse, rat for NELFB
	OriGene qPCR primer pairs and template standards for NELFB		OriGene Protein Over-expression Lysate for NELFB
	OriGene Custom Mass Spec		OriGene clones in human, mouse for NELFB
	OriGene qSTAR qPCR primer pairs in human, mouse for NELFB		Browse OriGene full length recombinant human proteins expressed in human HEK293 cells
	Browse OriGene ORF clones		OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
	OriGene Custom Antibody Services for NELFB		OriGene Custom Protein Services for NELFB

	QIAGEN Custom miScript Target Protector blocks miRNA-binding site of in human, mouse, rat NELFB
	QIAGEN SeqTarget long-range PCR primers for resequencing NELFB
	QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat NELFB
	QIAGEN FlexiTube/FlexiPlate siRNA for gene silencing in human, mouse, rat NELFB
	QIAGEN QuantiFast Probe-based Assays in human, mouse, rat NELFB
	QIAGEN QuantiTect SYBR Green Assays in human, mouse, rat NELFB

	GenScript Custom Purified and Recombinant Proteins Services for NELFB		GenScript Custom cDNA clones with any tag delivered in your preferred vector Services for NELFB
	GenScript Custom Assay Services for NELFB		GenScript Custom Superior Antibodies Services for NELFB
	GenScript Custom overexpressing Cell Line Services for NELFB		CloneReady with Over 120,000 Genes
	Gene Synthesis: Any Gene in Any Vector		Vector-based siRNA and miRNA, Ready for Transfection
	Gene Mutant Library, Variants up to 10^11		Plasmid Preparation
	Custom Peptide Services

Search for Antibodies & Assays

	Search Regulatory tfbs for NELFB
	Search Chromatin IP Primers for NELFB
	Search RT² qPCR Primer Assays in human, mouse, rat NELFB
	Search GNC Networks for NELFB
	SABiosciences Custom PCR Arrays for NELFB

Search Tocris compounds for NELFB

	Browse Sino Biological Proteins and Antibodies
	Browse Sino Biological Cell Lysates
	Browse Sino Biological cDNA Clones
	4000+ Proteins
	Search Sino Biological for antibodies, proteins & pathways
	Protein Production Services
	Transfection Reagents
	Protein A/G/L resins
	Isotyping reagents

Search www.enzolifesciences.com for proteins, assays, substrates, inhibitors & antibodies

	Novus Tissue Slides
	NELFB antibodies
	NELFB proteins
	NELFB lysates

	Antibodies for NELFB
	See all of Abcam's Antibodies, Kits and Proteins for NELFB
	Custom Antibody / Protein Production Service
	Bulk Purchasing
	Advantages of Rabbit Monoclonal antibodies
	Abcam protocols and scientific support

Browse ProSpec Recombinant Proteins

	Browse Proteins at Cloud-Clone Corp.
	Browse Antibodies at Cloud-Clone Corp.
	Browse ELISAs at Cloud-Clone Corp.
	Browse CLIAs at Cloud-Clone Corp.

Search LifeMap BioReagents cell lines for NELFB

	Gene Synthesis
	Protein Engineering
	Variant Library Synthesis
	Codon Optimization
	Protein Production and Purification

Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for NELFB

	SwitchGear 3'UTR luciferase reporter plasmids for NELFB
	SwitchGear Promoter luciferase reporter plasmids for NELFB

ThermoFisher Antibodies for NELFB

Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat NELFB

	inGenious Targeting Laboratory - Custom generated mouse model solutions for NELFB
	inGenious Targeting Laboratory - Custom generated inducible mouse model solutions for NELFB

Customized:

	lentivirus for stable overexpression of NELFB
	lentivirus expression plasmids for stable overexpression of NELFB
	adenovirus for overexpression of NELFB

Search LSBio for Antibodies for NELFB

Customized transgenic rodents for:

	Humanization
	Biomarker expression
	Off-target effect monitoring
	Translational medicine
	Tissue-specific gene expresssion
	Time-controlled gene expresssion

GeneCards Homepage - Last full update: 23 Oct 2013 - Incrementals: 4 Nov 2013 , 7 Nov 2013 , 23 Jan 2014

View Random Gene

Category			TGFB1 (GIFtS: 73) transforming growth factor, beta 1
GIFtS Group

The GeneCards human gene database gene index: 1 3 5 6 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z

The GeneCards Human Gene Database

Developed at the Crown Human Genome Center, Department of Molecular Genetics, the Weizmann Institute of Science

Hot genes Disease genes NELFB gene at Home site.

hostname: 356977-web1.xennexinc.com index build: 106 solr: 1.4

Advanced Search

Export list of displayed genes

NELFB Gene

Negative Elongation Factor Complex Member B

Aliases
for NELFB gene

Summaries
for NELFB gene

Genomic Views
for NELFB gene

Proteins
for NELFB gene

Protein Domains /
Families
for NELFB gene

Function
for NELFB gene

Pathways & Interactions
for NELFB gene

Drugs & Compounds
for NELFB gene

Transcripts
for NELFB gene

Expression
for NELFB gene

Orthologs
for NELFB gene

Paralogs
for NELFB gene

Genomic Variants
for NELFB gene

Disorders / Diseases
for NELFB gene

Publications
for NELFB gene

External Searches for NELFB gene

Genome Databases showing NELFB gene

Other Databases showing NELFB gene

Specialized Databases showing NELFB gene

Intellectual Property
for NELFB gene

Products
for NELFB gene

View Random Gene

Advanced Search

Export list of displayed genes

NELFB Gene

Negative Elongation Factor Complex Member B

Aliasesfor NELFB gene

Summariesfor NELFB gene

Genomic Viewsfor NELFB gene

Proteinsfor NELFB gene

Protein Domains /Familiesfor NELFB gene

Functionfor NELFB gene

Pathways & Interactionsfor NELFB gene

Drugs & Compoundsfor NELFB gene

Transcriptsfor NELFB gene

Expression for NELFB gene

Orthologsfor NELFB gene

Paralogsfor NELFB gene

Genomic Variantsfor NELFB gene

Disorders / Diseasesfor NELFB gene

Publicationsfor NELFB gene

External Searches for NELFB gene

Genome Databases showing NELFB gene

Other Databases showing NELFB gene

Specialized Databases showing NELFB gene

Intellectual Propertyfor NELFB gene

Productsfor NELFB gene

View Random Gene

Aliases
for NELFB gene

Summaries
for NELFB gene

Genomic Views
for NELFB gene

Proteins
for NELFB gene

Protein Domains /
Families
for NELFB gene

Function
for NELFB gene

Pathways & Interactions
for NELFB gene

Drugs & Compounds
for NELFB gene

Transcripts
for NELFB gene

Expression
for NELFB gene

Orthologs
for NELFB gene

Paralogs
for NELFB gene

Genomic Variants
for NELFB gene

Disorders / Diseases
for NELFB gene

Publications
for NELFB gene

Intellectual Property
for NELFB gene

Products
for NELFB gene