Set Analyses:
Advanced Search

Advanced Search

 
Search By
Section (entire)
for


 



NEK6 Gene

protein-coding   GIFtS: 65
GCID: GC09P127019

NIMA-Related Kinase 6

(Previous name: NIMA (never in mitosis gene a)-related kinase 6)
  Try our

disease database 

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
About This Section

TryGeneCards Plus

Aliases
NIMA-Related Kinase 61 2     Protein Kinase SID6-15122 3
NIMA (Never In Mitosis Gene A)-Related Kinase 61 2     EC 2.7.11.13 8
Putative Serine-Threonine Protein Kinase1 2     SID6-15122
Never In Mitosis A-Related Kinase 62 3     Serine/Threonine-Protein Kinase Nek62
NimA-Related Protein Kinase 62 3     

External Ids:    HGNC: 77491   Entrez Gene: 107832   Ensembl: ENSG000001194087   OMIM: 6048845   UniProtKB: Q9HC983   

Export aliases for NEK6 gene to outside databases

Previous GC identifers: GC09P118134 GC09P118666 GC09P120473 GC09P122395 GC09P122396 GC09P124099 GC09P126059 GC09P096633


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

TryGeneCards Plus

Entrez Gene summary for NEK6 Gene:
The protein encoded by this gene is a kinase required for progression through the metaphase portion of mitosis.
Inhibition of the encoded protein can lead to apoptosis. This protein also can enhance tumorigenesis by
suppressing tumor cell senescence. Several transcript variants encoding a few different isoforms have been found
for this gene. (provided by RefSeq, Oct 2011)

GeneCards Summary for NEK6 Gene:
NEK6 (NIMA-related kinase 6) is a protein-coding gene. GO annotations related to this gene include protein serine/threonine kinase activity and signal transducer activity. An important paralog of this gene is NEK5.

UniProtKB/Swiss-Prot: NEK6_HUMAN, Q9HC98
Function: Protein kinase which plays an important role in mitotic cell cycle progression. Required for chromosome
segregation at metaphase-anaphase transition, robust mitotic spindle formation and cytokinesis. Phosphorylates
ATF4, CIR1, PTN, RAD26L, RBBP6, RPS7, RPS6KB1, TRIP4, STAT3 and histones H1 and H3. Phosphorylates KIF11 to
promote mitotic spindle formation. Involved in G2/M phase cell cycle arrest induced by DNA damage. Inhibition of
activity results in apoptosis. May contribute to tumorigenesis by suppressing p53/TP53-induced cancer cell
senescence

Gene Wiki entry for NEK6 Gene


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
About This Section

TryGeneCards Plus
RefSeq DNA sequence at NCBI GenBank:
NC_000009.11  NT_008470.20  NC_018920.2  
Regulatory elements:
   Regulatory transcription factor binding sites in the NEK6 gene promoter:
         S8   USF1   USF-1   USF2   USF-1:USF-2   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmids (see all 2): NEK6 promoter sequence
   Search Chromatin IP Primers for NEK6

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat NEK6


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 9q33.3-q34.11   Ensembl cytogenetic band:  9q33.3   HGNC cytogenetic band: 9q33.3-q34.11

NEK6 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
NEK6 gene location

GeneLoc information about chromosome 9         GeneLoc Exon Structure

GeneLoc location for GC09P127019:  view genomic region     (about GC identifiers)

Start:
127,019,885 bp from pter      End:
127,115,586 bp from pter
Size:
95,702 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, Cloud-Clone Corp., and/or eBioscience,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, Cloud-Clone Corp., and/or eBioscience, Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, Cloud-Clone Corp, and/or others.)
About This Section

TryGeneCards Plus

UniProtKB/Swiss-Prot: NEK6_HUMAN, Q9HC98 (See protein sequence)
Recommended Name: Serine/threonine-protein kinase Nek6  
Size: 313 amino acids; 35714 Da
Cofactor: Magnesium
Subunit: Interacts with STAT3 and RPS6KB1 (By similarity). Interacts with NEK9, predominantly in mitosis.
Interacts with KIF11 (via C-terminus). Interacts with APBB1 (via WW domain). Interacts with ANKRA2, ATF4,
ARHGAP33, CDC42, CIR1, PRAM1, PTN, PRDX3, PIN1, RAD26L, RBBP6, RPS7 and TRIP4
Sequence caution: Sequence=AAG13417.1; Type=Miscellaneous discrepancy; Note=Contaminating sequence. Sequence of
unknown origin in the N-terminal part; Sequence=AAH00101.2; Type=Erroneous initiation; Note=Translation
N-terminally extended; Sequence=AAH04174.2; Type=Erroneous initiation; Note=Translation N-terminally extended;
Sequence=AAH04209.2; Type=Erroneous initiation; Note=Translation N-terminally extended; Sequence=BAA85045.1;
Type=Erroneous initiation; Note=Translation N-terminally extended;
Secondary accessions: B7Z2D9 Q5TBG3 Q5TBG9 Q6FG86 Q6IAR3 Q96E83 Q9ULX2
Alternative splicing: 4 isoforms:  Q9HC98-1   Q9HC98-2   Q9HC98-3   Q9HC98-4   

Explore the universe of human proteins at neXtProt for NEK6: NX_Q9HC98

Explore proteomics data for NEK6 at MOPED

Post-translational modifications: 

  • Autophosphorylated. Phosphorylation at Ser-206 is required for its activation. Phosphorylated upon IR or
    UV-induced DNA damage. Phosphorylated by CHEK1 and CHEK2. Interaction with APBB1 down-regulates phosphorylation
    at Thr-2101
  • Ubiquitination2 at Lys174
  • Modification sites at PhosphoSitePlus
  • Selected DME Specific Peptides for NEK6 (Q9HC98) (see all 9)
     HMPHGGS  GDLSQMIK  KPANVFIT  KSDIWSLGC 


    See NEK6 Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins (7 alternative transcripts): 
    NP_001138473.1  NP_001159639.1  NP_001159640.1  NP_001159641.1  NP_001159642.1  NP_001159643.1  NP_055212.2  

    ENSEMBL proteins: 
     ENSP00000362705   ENSP00000362702   ENSP00000319734   ENSP00000389517   ENSP00000405215  
     ENSP00000362698   ENSP00000403087   ENSP00000399847   ENSP00000411401   ENSP00000403414  
     ENSP00000441469   ENSP00000439651   ENSP00000377749   ENSP00000442636   ENSP00000441426  
    Reactome Protein details: Q9HC98

    NEK6 Human Recombinant Protein Products:

    EMD Millipore Purified and/or Recombinant NEK6 Protein
    Browse R&D Systems for human recombinant proteins
    Enzo Life Sciences proteins for NEK6
    OriGene Purified Proteins for NEK6
    OriGene Protein Over-expression Lysate for NEK6
    OriGene MassSpec for NEK6
    OriGene Custom Protein Services for NEK6
    GenScript Custom Purified and Recombinant Proteins Services for NEK6
    Novus Biologicals NEK6 Proteins
    Novus Biologicals NEK6 Lysates
    Browse Sino Biological Recombinant Proteins
    Browse Sino Biological Cell Lysates
    Browse ProSpec Recombinant Proteins
    Cloud-Clone Corp. Proteins for NEK6

     
    Search eBioscience for Proteins for NEK6 

    NEK6 Antibody Products:

    Browse EMD Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Browse R&D Systems for Antibodies
    OriGene Antibodies for NEK6
    OriGene Custom Antibody Services for NEK6
    Novus Biologicals NEK6 Antibodies
    Abcam antibodies for NEK6
    Cloud-Clone Corp. Antibodies for NEK6
    ThermoFisher Antibodies for NEK6
    LSBio Antibodies in human, mouse, rat for NEK6

    NEK6 Assay Products:

    Browse Kits and Assays available from EMD Millipore
    OriGene Custom Assay Services for NEK6
    Browse R&D Systems for biochemical assays
    GenScript Custom Assay Services for NEK6
    Browse Enzo Life Sciences for kits & assays
    Cloud-Clone Corp. ELISAs for NEK6
    Cloud-Clone Corp. CLIAs for NEK6
    Search eBioscience for ELISAs for NEK6 


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    TryGeneCards Plus
    IUPHAR Guide to PHARMACOLOGY protein family classification: NIMA-related kinase 6
    NIMA (never in mitosis gene a)- related kinase (NEK) family

    Selected InterPro protein domains (see all 6):
     IPR017441 Protein_kinase_ATP_BS
     IPR002290 Ser/Thr_dual-sp_kinase_dom
     IPR011009 Kinase-like_dom
     IPR008271 Ser/Thr_kinase_AS
     IPR000719 Prot_kinase_dom

    Graphical View of Domain Structure for InterPro Entry Q9HC98

    ProtoNet protein and cluster: Q9HC98

    UniProtKB/Swiss-Prot: NEK6_HUMAN, Q9HC98
    Domain: Displays an autoinhibited conformation: Tyr-108 side chain points into the active site, interacts with the
    activation loop, and blocks the alphaC helix. The autoinhibitory conformation is released upon binding with NEK9
    Similarity: Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily
    Similarity: Contains 1 protein kinase domain


    Find genes that share domains with NEK6           About GenesLikeMe


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, Vector BioLabs and/or Addgene, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Flow cytometry from eBioscience, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
    About This Section

    TryGeneCards Plus

    Molecular Function:

         UniProtKB/Swiss-Prot Summary: NEK6_HUMAN, Q9HC98
    Function: Protein kinase which plays an important role in mitotic cell cycle progression. Required for chromosome
    segregation at metaphase-anaphase transition, robust mitotic spindle formation and cytokinesis. Phosphorylates
    ATF4, CIR1, PTN, RAD26L, RBBP6, RPS7, RPS6KB1, TRIP4, STAT3 and histones H1 and H3. Phosphorylates KIF11 to
    promote mitotic spindle formation. Involved in G2/M phase cell cycle arrest induced by DNA damage. Inhibition of
    activity results in apoptosis. May contribute to tumorigenesis by suppressing p53/TP53-induced cancer cell
    senescence
    Catalytic activity: ATP + a protein = ADP + a phosphoprotein
    Enzyme regulation: Binding to NEK9 stimulates its activity by releasing the autoinhibitory function of Tyr-108
    Induction: Up-regulated during the M phase of cell cycle progression. Down-regulated in both replicative and
    premature senescence of cancer cells

         Enzyme Number (IUBMB): EC 2.7.11.11 2

         Gene Ontology (GO): Selected molecular function terms (see all 10):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000287magnesium ion binding IDA14563848
    GO:0004672protein kinase activity ----
    GO:0004674protein serine/threonine kinase activity IDA14563848
    GO:0004713protein tyrosine kinase activity ----
    GO:0004871signal transducer activity IMP12761501
         
    Find genes that share ontologies with NEK6           About GenesLikeMe


    Phenotypes:
         4 GenomeRNAi human phenotypes for NEK6:
     Decreased telomerase activity  Decreased viability of wild-ty  Increased cell death HMECs cel  Increased number of small and  

         1 MGI mutant phenotype (inferred from 2 alleles(MGI details for Nek6):
     normal 

    Find genes that share phenotypes with NEK6           About GenesLikeMe

    Animal Models:
       inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for NEK6
       inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for NEK6

       genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for NEK6
       genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for NEK6

    miRNA
    Products:
        
    miRTarBase miRNAs that target NEK6:
    hsa-mir-92a-3p (MIRT049722), hsa-mir-1307-3p (MIRT035813), hsa-mir-24-3p (MIRT030523), hsa-mir-124-3p (MIRT002611)

    Block miRNA regulation of human, mouse, rat NEK6 using miScript Target Protectors
    Selected qRT-PCR Assays for microRNAs that regulate NEK6 (see all 30):
    hsa-miR-3685 hsa-miR-323-3p hsa-miR-188-5p hsa-miR-513a-5p hsa-miR-128 hsa-miR-200a hsa-miR-500a hsa-miR-340
    SwitchGear 3'UTR luciferase reporter plasmidNEK6 3' UTR sequence
    Inhib. RNA
    Products:
        
    OriGene RNAi products in human, mouse, rat for NEK6
    Predesigned siRNA for gene silencing in human, mouse, rat NEK6

    Gene Editing
    Products:
    DNA2.0 Custom Protein Engineering Service for NEK6

    Clone
    Products:
         
    OriGene clones in human, mouse for NEK6 (see all 39)
    OriGene ORF clones in mouse, rat for NEK6
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector (see all 7): NEK6 (NM_001145001)
    Sino Biological Human cDNA Clone for NEK6
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for NEK6
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat NEK6
    Addgene plasmids for NEK6 

    Cell Line
    Products:
         
    GenScript Custom overexpressing Cell Line Services for NEK6
    Browse ESI BIO Cell Lines and PureStem Progenitors for NEK6 
    In Situ Assay
    Products:
       

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for NEK6


    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
    About This Section

    TryGeneCards Plus

    Subcellular locations from UniProtKB/Swiss-Prot
    NEK6_HUMAN, Q9HC98: Cytoplasm. Nucleus. Nucleus speckle. Cytoplasm, cytoskeleton, microtubule organizing center,
    centrosome. Cytoplasm, cytoskeleton, spindle pole. Note=Colocalizes with APBB1 at the nuclear speckles.
    Colocalizes with PIN1 in the nucleus. Colocalizes with ATF4, CIR1, ARHGAP33, ANKRA2, CDC42, NEK9, RAD26L, RBBP6,
    RPS7, TRIP4, RELB and PHF1 in the centrosome. Localizes to spindle microtubules in metaphase and anaphase and to
    the midbody during cytokinesis
    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    cytoskeleton5
    nucleus5
    cytosol4

    Gene Ontology (GO): Selected cellular component terms (see all 7):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000922spindle pole IEA--
    GO:0005634nucleus IDA12054534
    GO:0005737cytoplasm IDA12054534
    GO:0005815microtubule organizing center IEA--
    GO:0005829cytosol TAS--

    Find genes that share ontologies with NEK6           About GenesLikeMe


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
    About This Section

    TryGeneCards Plus

    SuperPaths for NEK6 About    
    See pathways by source

    SuperPathContained pathways About
    1Cell Cycle, Mitotic
    Cell Cycle, Mitotic0.90
    M Phase0.62
    Cell Cycle0.90
    2Regulation of Glucokinase by Glucokinase Regulatory Protein
    Nuclear Pore Complex (NPC) Disassembly0.77
    Activation of NIMA Kinases NEK9, NEK6, NEK70.00
    Nuclear Envelope Breakdown0.77
    3RNA Polymerase I Promoter Opening
    Mitotic Prophase0.56


    Find genes that share SuperPaths with NEK6           About GenesLikeMe

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways



    2 Reactome Pathways for NEK6
        Nuclear Pore Complex (NPC) Disassembly
    Activation of NIMA Kinases NEK9, NEK6, NEK7


        Pathway & Disease-focused RT2 Profiler PCR Array including NEK6: 
              Epigenetic Chromatin Modification Enzymes in human mouse rat

    Interactions:

        GeneGlobe Interaction Network for NEK6

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    Selected Interacting proteins for NEK6 (Q9HC981, 2, 3 ENSP000003627024) via UniProtKB, MINT, STRING, and/or I2D (see all 99)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    CCDC85BQ158342, 3, ENSP000003116954MINT-66075 I2D: score=4 STRING: ENSP00000311695
    PIN1Q135261, 3, ENSP000002479704EBI-740364,EBI-714158 I2D: score=2 STRING: ENSP00000247970
    NECAB2Q7Z6G32, 3, ENSP000003074494MINT-67753 I2D: score=3 STRING: ENSP00000307449
    EML3Q32P443, ENSP000003782544I2D: score=2 STRING: ENSP00000378254
    SMC3Q9UQE73, ENSP000003547204I2D: score=2 STRING: ENSP00000354720
    About this table

    Gene Ontology (GO): Selected biological process terms (see all 16):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000278mitotic cell cycle TAS--
    GO:0000910cytokinesis TAS--
    GO:0006468protein phosphorylation IDA12840024
    GO:0006915apoptotic process IEA--
    GO:0007059chromosome segregation IDA14563848

    Find genes that share ontologies with NEK6           About GenesLikeMe



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
    About This Section

    TryGeneCards Plus
    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
      Browse compounds at ApexBio 

    Browse Tocris compounds for NEK6

    3 HMDB Compounds for NEK6    About this table
    CompoundSynonyms CAS #PubMed Ids
    ADPadenosindiphosphorsaeure (see all 8)58-64-0--
    Adenosine triphosphate5'-(tetrahydrogen triphosphate) Adenosine (see all 24)56-65-5--
    MagnesiumMagnesium (see all 2)7439-95-4--



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, and/or Addgene, Primers from OriGene, and/or QIAGEN, Flow cytometry from eBioscience )
    About This Section

    TryGeneCards Plus

    REFSEQ mRNAs for NEK6 gene (7 alternative transcripts): 
    NM_001145001.2  NM_001166167.1  NM_001166168.1  NM_001166169.1  NM_001166170.1  NM_001166171.1  NM_014397.5  

    Unigene Cluster for NEK6:

    NIMA-related kinase 6
    Hs.197071  [show with all ESTs]
    Unigene Representative Sequence: AL832574
    15 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000373603(uc004bof.3) ENST00000373600(uc004boh.3 uc004bog.3)
    ENST00000320246 ENST00000444973 ENST00000454453 ENST00000373596 ENST00000425237
    ENST00000423785 ENST00000422297 ENST00000447379 ENST00000540326 ENST00000539416(uc022bng.1)
    ENST00000394199(uc022bnf.1) ENST00000545174(uc010mwk.3) ENST00000546191(uc004boi.3)

    miRNA
    Products:
         
    Block miRNA regulation of human, mouse, rat NEK6 using miScript Target Protectors
    Selected qRT-PCR Assays for microRNAs that regulate NEK6 (see all 30):
    hsa-miR-3685 hsa-miR-323-3p hsa-miR-188-5p hsa-miR-513a-5p hsa-miR-128 hsa-miR-200a hsa-miR-500a hsa-miR-340
    SwitchGear 3'UTR luciferase reporter plasmidNEK6 3' UTR sequence
    Inhib. RNA
    Products:
         
    OriGene RNAi products in human, mouse, rat for NEK6
    Predesigned siRNA for gene silencing in human, mouse, rat NEK6
    Clone
    Products:
         
    OriGene clones in human, mouse for NEK6 (see all 39)
    OriGene ORF clones in mouse, rat for NEK6
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector (see all 7): NEK6 (NM_001145001)
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for NEK6
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat NEK6
    Addgene plasmids for NEK6 
    Primer
    Products:
        
    OriGene qPCR primer pairs and template standards for NEK6
    OriGene qSTAR qPCR primer pairs in human, mouse for NEK6
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat NEK6
      QuantiTect SYBR Green Assays in human, mouse, rat NEK6
      QuantiFast Probe-based Assays in human, mouse, rat NEK6

    Additional mRNA sequence: 

    AB026289.1 AF087909.1 AJ420460.1 AK294614.1 AK313071.1 AL832574.2 AY567967.1 BC000101.2 
    BC004174.2 BC004209.2 BC012761.2 CR457091.1 CR542222.1 

    21 DOTS entries:

    DT.85105540  DT.451785  DT.100793525  DT.99938623  DT.100793524  DT.100793530  DT.99934741  DT.100793528 
    DT.121148913  DT.404604  DT.121148918  DT.121148643  DT.99944232  DT.121148712  DT.100793523  DT.100805605 
    DT.121148931  DT.121148940  DT.86851328  DT.95338501  DT.97845843 

    Selected AceView cDNA sequences (see all 341):

    AI984544 BM722773 T12550 AA600235 T12624 AW376434 AW268702 D20396 
    BG685480 BQ028289 BQ669719 BU728759 AI377235 BI021102 CA418621 AI673193 
    CA426656 AA291952 AA451721 BI756997 T12555 AA493426 BM765808 CR608265 

    GeneLoc Exon Structure

    Selected Alternative Splicing Database (ASD) splice patterns (SP) for NEK6 (see all 8)    About this scheme

    ExUns: 1 ^ 2 ^ 3 ^ 4a · 4b · 4c ^ 5 ^ 6 ^ 7 ^ 8 ^ 9 ^ 10 ^ 11a · 11b ^ 12 ^ 13 ^ 14 ^ 15 ^ 16a · 16b · 16c
    SP1:              -     -     -     -     -           -                                                                           
    SP2:        -     -     -     -     -     -     -     -                                                                           
    SP3:              -     -     -     -     -     -     -                                                                           
    SP4:                                                                                                                              
    SP5:                    -     -     -     -     -     -                                                                           


    ECgene alternative splicing isoforms for NEK6

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    TryGeneCards Plus

    NEK6 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: TCACTGATGG
    NEK6 Expression
    About this image


    NEK6 expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database
     selected tissues (see all 4) fully expand
     
     Brain (Nervous System)    fully expand to see all 5 entries
             Cerebral Cortex
     
     Neural Tube (Nervous System)    fully expand to see all 3 entries
             Metencephalon
     
     Lung (Respiratory System)    fully expand to see all 2 entries
             Stem Bronchi
     
     Gut Tube (Gastrointestinal Tract)
             Foregut
    NEK6 Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    NEK6 Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.197071

    UniProtKB/Swiss-Prot: NEK6_HUMAN, Q9HC98
    Tissue specificity: Ubiquitous, with highest expression in heart and skeletal muscle. Up-regulated in a variety of
    malignant cancers, such as breast, colon, lung, and gastric cancers

        Pathway & Disease-focused RT2 Profiler PCR Array including NEK6: 
              Epigenetic Chromatin Modification Enzymes in human mouse rat

    Primer
    Products:
    OriGene qPCR primer pairs and template standards for NEK6
    OriGene qSTAR qPCR primer pairs in human, mouse for NEK6
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat NEK6
    QuantiTect SYBR Green Assays in human, mouse, rat NEK6
    QuantiFast Probe-based Assays in human, mouse, rat NEK6
    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for NEK6

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    TryGeneCards Plus

    This gene was present in the common ancestor of animals and fungi.

    Orthologs for NEK6 gene from Selected species (see all 15)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Nek61 , 5 NIMA (never in mitosis gene a)-related expressed kinase more1, 5 88.71(n)1
    95.21(a)1
      2 (24.41 cM)5
    591261  NM_001159631.11  NP_001153103.11 
     385116435 
    chicken
    (Gallus gallus)
    Aves NEK61 NIMA (never in mitosis gene a)-related kinase 6 75.72(n)
    88.82(a)
      417101  NM_001012531.1  NP_001012549.1 
    lizard
    (Anolis carolinensis)
    Reptilia NEK66
    NIMA-related kinase 6
    73(a)
    1 ↔ 1
    GL344570.1(2973-39201)
    African clawed frog
    (Xenopus laevis)
    Amphibia nek6-prov2 NIMA (never in mitosis gene a)-related kinase 6 79.83(n)    AY271413.1 
    zebrafish
    (Danio rerio)
    Actinopterygii nek61 NIMA (never in mitosis gene a)-related kinase 6 76.31(n)
    83.52(a)
      497165  XM_005161494.1  XP_005161551.1 
    worm
    (Caenorhabditis elegans)
    Secernentea nekl-36
    Protein NEKL-3 (nekl-3) mRNA, complete cds
    66(a)
    1 → many
    X(12389594-12391474) WBGene00008956
    baker's yeast
    (Saccharomyces cerevisiae)
    Saccharomycetes KIN36
    Nonessential serine/threonine protein kinase; poss...
    23(a)
    1 → many
    I(170396-171703) YAR018C


    ENSEMBL Gene Tree for NEK6 (if available)
    TreeFam Gene Tree for NEK6 (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section

    TryGeneCards Plus
    Paralogs for NEK6 gene
    NEK52  NEK82  NEK22  NEK42  NEK102  NEK12  NEK112  NEK32  
    NEK92  NEK72  
    Selected SIMAP similar genes for NEK6 using alignment to 8 protein entries:     NEK6_HUMAN (see all proteins) (see all similar genes):
    NEK7    MST094    NEK8    DKFZp564L2416    NEK1    NEK2
    NEK5    DKFZp434B079    NEK3    NEK9    ABL1    CDKL3
    MAP2K2    NEK10    NEK4    MYLK    NEK11    MAP3K6

    Find genes that share paralogs with NEK6           About GenesLikeMe



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
    About This Section

    TryGeneCards Plus

    Selected SNPs for NEK6 (see all 1982)    About this table                                 

    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 9 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1385930361,2
    C--96631385(+) CTTCCA/GAGTTA 2 -- int10--------
    rs1492849571,2
    --96631477(+) ATCTCA/GGCTCA 2 -- int10--------
    rs123401781,2
    --96631595(+) gtagaC/Gacagg 1 -- us2k10--------
    rs1381952731,2
    --96631696(+) GCCACC/GGCGCC 1 -- us2k10--------
    rs109862931,2
    C,F--96631699(+) ACCGCG/ACCCGG 1 -- us2k14Minor allele frequency- A:0.25NA WA CSA 8
    rs1472388551,2
    C--96631702(+) GCGCCC/TGGCCC 1 -- us2k10--------
    rs1812306951,2
    --96631775(+) TGACTA/GAAGCC 1 -- us2k10--------
    rs713729551,2
    C--127018060(+) TTTTTT/-GGAGA 1 -- us2k11Minor allele frequency- -:0.00NA 2
    rs1406873271,2
    --127018938(+) CTGCCA/GTGCAC 4 -- us2k10--------
    rs109862941,2
    C,F--127019113(+) TGCAGG/ACAGGC 4 -- us2k12Minor allele frequency- A:0.20NA EA 240

    HapMap Linkage Disequilibrium report for NEK6 (127019885 - 127115586 bp)

    Structural Variations
         Database of Genomic Variants (DGV) 10 variations for NEK6:    About this table    
    Variant IDTypeSubtypePubMed ID
    esv2739035CNV Deletion23290073
    esv2739034CNV Deletion23290073
    esv2677076CNV Deletion23128226
    nsv519767CNV Loss19592680
    nsv6711CNV Loss18451855
    nsv831720CNV Loss17160897
    nsv471322CNV Loss18288195
    nsv893833CNV Gain21882294
    nsv831719CNV Gain17160897
    nsv825074CNV Gain20364138

    Site Specific Mutation Identification with PCR Assays
    SeqTarget long-range PCR primers for resequencing NEK6
    DNA2.0 Custom Variant and Variant Library Synthesis for NEK6

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    TryGeneCards Plus
    OMIM gene information: 604884    OMIM disorders: --

    1 disease from the University of Copenhagen DISEASES database for NEK6:
    Myotonic dystrophy

    Find genes that share disorders with NEK6           About GenesLikeMe

    Genetic Association Database (GAD): NEK6
    Human Genome Epidemiology (HuGE) Navigator: NEK6 (2 documents)

    Export disorders for NEK6 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    TryGeneCards Plus

    PubMed articles for NEK6 gene, integrated from 10 sources (see all 46):
    (articles sorted by number of sources associating them with NEK6)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Human NIMA-related kinase 6 is one of the Fe65 WW domain binding proteins. (PubMed id 17512906)1, 2, 9 Lee E.J.... Kang S.S. (Biochem. Biophys. Res. Commun. 2007)
    2. The serine/threonine kinase Nek6 is required for cell cycle progression through mitosis. (PubMed id 14563848)1, 2, 9 Yin M.J.... Jallal B. (J. Biol. Chem. 2003)
    3. Interaction of Pin1 with Nek6 and characterization of their expression correlation in Chinese hepatocellular carcinoma patients. (PubMed id 16476580)1, 2, 9 Chen J.... Yu L. (Biochem. Biophys. Res. Commun. 2006)
    4. Common genetic variation and performance on standardized cognitive tests. (PubMed id 20125193)1, 4 Cirulli E.T....Goldstein D.B. (Eur. J. Hum. Genet. 2010)
    5. An approach based on a genome-wide association study reveals candidate loci for narcolepsy. (PubMed id 20677014)1, 4 Shimada M....Tokunaga K. (Hum. Genet. 2010)
    6. Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. (PubMed id 20379614)1, 4 Rose J.E....Uhl G.R. (Mol. Med. 2010)
    7. Nek6 overexpression antagonizes p53-induced senescence in human cancer cells. (PubMed id 21099361)1, 2 Jee H.J.... Yun J. (Cell Cycle 2010)
    8. Nek6 mediates human cancer cell transformation and is a potential cancer therapeutic target. (PubMed id 20407017)1, 2 Nassirpour R....Yin M.J. (Mol. Cancer Res. 2010)
    9. Characterization of hNek6 interactome reveals an important role for its short N-terminal domain and colocalization with proteins at the centrosome. (PubMed id 20873783)1, 2 Vaz Meirelles G.... Kobarg J. (J. Proteome Res. 2010)
    10. The Nek6 and Nek7 protein kinases are required for robust mitotic spindle formation and cytokinesis. (PubMed id 19414596)1, 2 O'Regan L. and Fry A.M. (Mol. Cell. Biol. 2009)

    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

    TryGeneCards Plus
     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section

    TryGeneCards Plus
    Entrez Gene: 10783 HGNC: 7749 AceView: NEK6 Ensembl:ENSG00000119408 euGenes: HUgn10783
    ECgene: NEK6 H-InvDB: NEK6

    (According to HUGE)
    About This Section

    TryGeneCards Plus
      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
    About This Section

    TryGeneCards Plus
    NameDescription
    PharmGKB entry for NEK6 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section

    TryGeneCards Plus
    Patent Information for NEK6 gene:
    Search GeneIP for patents involving NEK6

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, eBioscience, and/or others, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Addgene, Cell lines from GenScript, and ESI BIO, Flow cytometery from eBioscience, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
    Advanced Cell Diagnostics, Animal models from inGenious Targeting Laboratory, genOway)
    About This Section

    TryGeneCards Plus

     EMD Millipore Purified and/or Recombinant NEK6 Protein
     Browse Small Molecules at EMD Millipore
     Browse EMD Millipore's Extensive Line of Mono- and Polyclonal Antibodies
     Browse Kits and Assays available from EMD Millipore
     
     EMD Millipore genomic analysis products

      
     Browse Antibodies   Browse Cell Culture Products  
     Browse ELISAs   Browse Flow Cytometry Kits  
     Browse Primer Pairs   Browse Enzyme Activity Assays/Reagents  
     Browse ELISpot/FluoroSpot Kits/Development Modules   Browse TFB/Immunoprecipitation Assays  
     Browse Apoptosis Detection Kits/Reagents   Browse Ubiquitin Proteasome Pathway (UPP) Assay Kits/Reagents  
     Browse DNA Damage/Repair Kits/Reagents   Browse Luminex Assays  
     Browse Cell Selection/Detection Kits/Reagents   Browse Secondary Antibodies/Controls/Staining Reagents  
     Browse Recombinant/Natural Proteins   Browse Stem Cell Products  
     Browse cDNA Clones   Browse Proteome Profiler Antibody Arrays  
     OriGene Antibodies for NEK6   OriGene RNAi products in human, mouse, rat for NEK6  
     OriGene qPCR primer pairs and template standards for NEK6   OriGene Protein Over-expression Lysate for NEK6  
     OriGene MassSpec something-or-other for NEK6   OriGene clones in human, mouse for NEK6  
     OriGene qSTAR qPCR primer pairs in human, mouse for NEK6   OriGene Purified Protein for NEK6  
     OriGene ORF clones in mouse, rat for NEK6   OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling  
     OriGene Custom Antibody Services for NEK6   OriGene Custom Protein Services for NEK6  

     
     
     Block miRNA regulation of human, mouse, rat NEK6 using miScript Target Protectors SeqTarget long-range PCR primers for resequencing NEK6
     DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat NEK6 Predesigned siRNA for gene silencing in human, mouse, rat NEK6
     QuantiFast Probe-based Assays in human, mouse, rat NEK6 QuantiTect SYBR Green Assays in human, mouse, rat NEK6
     PCR Arrays including human, mouse, rat NEK6 Search Chromatin IP Primers for NEK6
     Pre-validated RT2 qPCR Primer Assay in human, mouse, rat NEK6  GeneGlobe Interaction Network for NEK6
     Regulatory tfbs in NEK6 promoter
     GenScript Custom Purified and Recombinant Proteins Services for NEK6 GenScript cDNA clones with any tag delivered in your preferred vector for NEK6
     GenScript Custom Assay Services for NEK6 GenScript Custom overexpressing Cell Line Services for NEK6
     CloneReady with Over 120,000 Genes  Gene Synthesis: Any Gene in Any Vector
     Vector-based siRNA and miRNA, Ready for Transfection Gene Mutant Library, Variants up to 10^11
     Plasmid Preparation Custom Peptide Services
     Search for Antibodies & Assays

     Search Tocris compounds for NEK6
     Browse Sino Biological Proteins
     Browse Sino Biological Cell Lysates
     cDNA Clones for NEK6
     4000+ Proteins
     Search Sino Biological for antibodies, proteins & pathways
     Protein Production Services
     Transfection Reagents
     Protein A/G/L resins
     Isotyping reagents
     Proteins for NEK6
     Search www.enzolifesciences.com for proteins, assays, substrates, inhibitors & antibodies

     Novus Tissue Slides
     NEK6 antibodies
     NEK6 proteins
     NEK6 lysates
     Antibodies for NEK6
     See all of Abcam's Antibodies, Kits and Proteins for NEK6
     Custom Antibody / Protein Production Service
     Bulk Purchasing
     Advantages of Rabbit Monoclonal antibodies
     Abcam protocols and scientific support
     Browse ProSpec Recombinant Proteins
     Proteins for NEK6
     Antibodies for NEK6
     ELISAs for NEK6
     CLIAs for NEK6



     Browse ESI BIO Cell Lines and PureStem Progenitors for NEK6
     Gene Synthesis
     Protein Engineering
     Variant Library Synthesis
     Codon Optimization
     Protein Production and Purification
     Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for NEK6
     SwitchGear 3'UTR luciferase reporter plasmids for NEK6
     SwitchGear Promoter luciferase reporter plasmids for NEK6
     ThermoFisher Antibodies for NEK6
     Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat NEK6
     inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for NEK6
     inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for NEK6
     LSBio Antibodies in human, mouse, rat for NEK6
    Customized transgenic rodents for:
     Humanization
     Biomarker expression
     Off-target effect monitoring
     Translational medicine
     Tissue-specific gene expresssion
     Time-controlled gene expresssion
     Browse compounds at ApexBio
     Addgene plasmids for NEK6
      Search eBioscience for proteins for NEK6
      Search eBioscience for elisas for NEK6
      eBioscience FlowRNA Probe Sets
           
    GeneCards Homepage - Last full update: 7 May 2014 - Incrementals: 9 May 2014 , 2 Jun 2014 , 26 Jun 2014 , 30 Jun 2014 , 21 Aug 2014 , 8 Sep 2014

    View Random Gene

    Category
    (GIFtS: )
    GIFtS Group
    The GeneCards human gene database gene index: 1 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 


    Developed at the Crown Human Genome Center, Department of Molecular Genetics, the Weizmann Institute of Science

    Hot genes      Disease genes      NEK6 gene at Home site.
    Version: 3.12.224 13 Oct 2014
    hostname: 356977-web1.xennexinc.com index build: 128 solr: 1.4