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Aliases for NEK6 Gene

Aliases for NEK6 Gene

  • NIMA Related Kinase 6 2 3 5
  • NIMA (Never In Mitosis Gene A)-Related Kinase 6 2 3
  • Putative Serine-Threonine Protein Kinase 2 3
  • Never In Mitosis A-Related Kinase 6 3 4
  • NimA-Related Protein Kinase 6 3 4
  • Protein Kinase SID6-1512 3 4
  • EC 2.7.11.1 4 56
  • Serine/Threonine-Protein Kinase Nek6 3
  • SID6-1512 3

External Ids for NEK6 Gene

Previous GeneCards Identifiers for NEK6 Gene

  • GC09P118134
  • GC09P118666
  • GC09P120473
  • GC09P122395
  • GC09P122396
  • GC09P124099
  • GC09P126059
  • GC09P127019
  • GC09P096633

Summaries for NEK6 Gene

Entrez Gene Summary for NEK6 Gene

  • The protein encoded by this gene is a kinase required for progression through the metaphase portion of mitosis. Inhibition of the encoded protein can lead to apoptosis. This protein also can enhance tumorigenesis by suppressing tumor cell senescence. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

GeneCards Summary for NEK6 Gene

NEK6 (NIMA Related Kinase 6) is a Protein Coding gene. Among its related pathways are Mitotic Prophase and Transport of the SLBP independent Mature mRNA. Gene Ontology (GO) annotations related to this gene include transferase activity, transferring phosphorus-containing groups and protein tyrosine kinase activity. An important paralog of this gene is NEK7.

UniProtKB/Swiss-Prot for NEK6 Gene

  • Protein kinase which plays an important role in mitotic cell cycle progression. Required for chromosome segregation at metaphase-anaphase transition, robust mitotic spindle formation and cytokinesis. Phosphorylates ATF4, CIR1, PTN, RAD26L, RBBP6, RPS7, RPS6KB1, TRIP4, STAT3 and histones H1 and H3. Phosphorylates KIF11 to promote mitotic spindle formation. Involved in G2/M phase cell cycle arrest induced by DNA damage. Inhibition of activity results in apoptosis. May contribute to tumorigenesis by suppressing p53/TP53-induced cancer cell senescence.

Gene Wiki entry for NEK6 Gene

Additional gene information for NEK6 Gene

No data available for CIViC summary , Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for NEK6 Gene

Genomics for NEK6 Gene

GeneHancer (GH) Regulatory Elements for NEK6 Gene

Promoters and enhancers for NEK6 Gene
GeneHancer (GH) Identifier GH Type GH
Score
GH Sources Gene Association Score Total Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor
Binding Sites
Gene Targets
GH09I124256 Promoter/Enhancer 2.5 EPDnew FANTOM5 Ensembl ENCODE dbSUPER 569.4 +5.1 5076 13.3 HDGF PKNOX1 CLOCK FOXA2 ZFP64 ARID4B SIN3A DMAP1 IRF4 YY1 NEK6 LOC613206 ENSG00000227200 DENND1A
GH09I124277 Promoter/Enhancer 2.2 EPDnew FANTOM5 Ensembl ENCODE dbSUPER 575.6 +32.2 32153 24.7 HDGF PKNOX1 FOXA2 MLX ARID4B SIN3A YBX1 IRF4 YY1 ZNF766 NEK6 DENND1A LOC105376269 PIR33879
GH09I124220 Enhancer 1.6 FANTOM5 Ensembl ENCODE dbSUPER 31.3 -35.4 -35406 4.4 FOXA2 MLX ARID4B DMAP1 ZNF48 ETS1 YY1 FOS RXRA SP5 NEK6 DENND1A GC09M124203
GH09I124215 Enhancer 1.5 FANTOM5 Ensembl ENCODE dbSUPER 17.8 -40.0 -40005 4.4 HDGF PKNOX1 FOXA2 IRF4 ZNF48 ZNF207 ZNF143 ATF7 FOS RUNX3 NEK6 RPL35 DENND1A GC09M124203
GH09I124414 Promoter/Enhancer 2.3 EPDnew Ensembl ENCODE dbSUPER 11.6 +157.5 157460 2.1 HDGF HNRNPUL1 PKNOX1 FOXA2 SMAD1 ARNT ARID4B SIN3A FEZF1 DMAP1 PSMB7 GAPVD1 GOLGA1 PRPS1P2 NEK6 GC09P124378
- Elite GeneHancer and/or Elite GeneHancer-gene association Download GeneHancer data dump

GeneHancers around NEK6 on UCSC Golden Path with GeneCards custom track

Top Transcription factor binding sites by QIAGEN in the NEK6 gene promoter:

Genomic Locations for NEK6 Gene

Genomic Locations for NEK6 Gene
chr9:124,257,606-124,378,316
(GRCh38/hg38)
Size:
120,711 bases
Orientation:
Plus strand
chr9:127,019,885-127,115,586
(GRCh37/hg19)

Genomic View for NEK6 Gene

Genes around NEK6 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
NEK6 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for NEK6 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for NEK6 Gene

Proteins for NEK6 Gene

  • Protein details for NEK6 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q9HC98-NEK6_HUMAN
    Recommended name:
    Serine/threonine-protein kinase Nek6
    Protein Accession:
    Q9HC98
    Secondary Accessions:
    • B7Z2D9
    • Q5TBG3
    • Q5TBG9
    • Q6FG86
    • Q6IAR3
    • Q96E83
    • Q9ULX2

    Protein attributes for NEK6 Gene

    Size:
    313 amino acids
    Molecular mass:
    35714 Da
    Cofactor:
    Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
    Quaternary structure:
    • Interacts with STAT3 and RPS6KB1 (By similarity). Interacts with NEK9, predominantly in mitosis. Interacts with KIF11 (via C-terminus). Interacts with APBB1 (via WW domain). Interacts with ANKRA2, ATF4, ARHGAP33, CDC42, CIR1, PRAM1, PTN, PRDX3, PIN1, RAD26L, RBBP6, RPS7 and TRIP4.
    SequenceCaution:
    • Sequence=AAG13417.1; Type=Miscellaneous discrepancy; Note=Contaminating sequence. Sequence of unknown origin in the N-terminal part.; Evidence={ECO:0000305}; Sequence=AAH00101.2; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305}; Sequence=AAH04174.2; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305}; Sequence=AAH04209.2; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305}; Sequence=BAA85045.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305};

    Alternative splice isoforms for NEK6 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for NEK6 Gene

Selected DME Specific Peptides for NEK6 Gene

Q9HC98:
  • IEKKIGRGQFSEVY
  • GDLSQMIK
  • HMPHGGS
  • GTPYYMSPE
  • FKKQKRLIPERTVWKYFVQLCSA
  • KSDIWSLGC
  • KIEQCDYPPLP
  • KPANVFIT
  • SFIEDNELNIVLELADAGDLS

Post-translational modifications for NEK6 Gene

  • Autophosphorylated. Phosphorylation at Ser-206 is required for its activation. Phosphorylated upon IR or UV-induced DNA damage. Phosphorylated by CHEK1 and CHEK2. Interaction with APBB1 down-regulates phosphorylation at Thr-210.

Domains & Families for NEK6 Gene

Gene Families for NEK6 Gene

IUPHAR :
Human Protein Atlas (HPA):
  • Enzymes
  • Predicted intracellular proteins
  • Transporters

Suggested Antigen Peptide Sequences for NEK6 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

Q9HC98

UniProtKB/Swiss-Prot:

NEK6_HUMAN :
  • Displays an autoinhibited conformation: Tyr-108 side chain points into the active site, interacts with the activation loop, and blocks the alphaC helix. The autoinhibitory conformation is released upon binding with NEK9.
  • Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
Domain:
  • Displays an autoinhibited conformation: Tyr-108 side chain points into the active site, interacts with the activation loop, and blocks the alphaC helix. The autoinhibitory conformation is released upon binding with NEK9.
Family:
  • Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
genes like me logo Genes that share domains with NEK6: view

Function for NEK6 Gene

Molecular function for NEK6 Gene

UniProtKB/Swiss-Prot CatalyticActivity:
ATP + a protein = ADP + a phosphoprotein.
UniProtKB/Swiss-Prot EnzymeRegulation:
Binding to NEK9 stimulates its activity by releasing the autoinhibitory function of Tyr-108.
UniProtKB/Swiss-Prot Function:
Protein kinase which plays an important role in mitotic cell cycle progression. Required for chromosome segregation at metaphase-anaphase transition, robust mitotic spindle formation and cytokinesis. Phosphorylates ATF4, CIR1, PTN, RAD26L, RBBP6, RPS7, RPS6KB1, TRIP4, STAT3 and histones H1 and H3. Phosphorylates KIF11 to promote mitotic spindle formation. Involved in G2/M phase cell cycle arrest induced by DNA damage. Inhibition of activity results in apoptosis. May contribute to tumorigenesis by suppressing p53/TP53-induced cancer cell senescence.
UniProtKB/Swiss-Prot Induction:
Up-regulated during the M phase of cell cycle progression. Down-regulated in both replicative and premature senescence of cancer cells.

Enzyme Numbers (IUBMB) for NEK6 Gene

Phenotypes From GWAS Catalog for NEK6 Gene

Gene Ontology (GO) - Molecular Function for NEK6 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000287 magnesium ion binding IDA 14563848
GO:0001222 transcription corepressor binding IPI 20873783
GO:0004672 protein kinase activity IEA --
GO:0004674 protein serine/threonine kinase activity TAS --
GO:0004871 signal transducer activity HMP,IMP 12761501
genes like me logo Genes that share ontologies with NEK6: view
genes like me logo Genes that share phenotypes with NEK6: view

Animal Models for NEK6 Gene

MGI Knock Outs for NEK6:

Animal Model Products

Clone Products

  • Addgene plasmids for NEK6

No data available for Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for NEK6 Gene

Localization for NEK6 Gene

Subcellular locations from UniProtKB/Swiss-Prot for NEK6 Gene

Cytoplasm. Nucleus. Nucleus speckle. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole. Note=Colocalizes with APBB1 at the nuclear speckles. Colocalizes with PIN1 in the nucleus. Colocalizes with ATF4, CIR1, ARHGAP33, ANKRA2, CDC42, NEK9, RAD26L, RBBP6, RPS7, TRIP4, RELB and PHF1 in the centrosome. Localizes to spindle microtubules in metaphase and anaphase and to the midbody during cytokinesis.

Subcellular locations from

COMPARTMENTS
Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for NEK6 gene
Compartment Confidence
cytoskeleton 5
nucleus 5
cytosol 5

Subcellular locations from the

Human Protein Atlas (HPA)
  • Cytosol (3)
  • Microtubule organizing center (3)
  • Nucleoplasm (3)
See all subcellular structures

Gene Ontology (GO) - Cellular Components for NEK6 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000922 spindle pole IEA --
GO:0005634 nucleus IEA,IDA 12054534
GO:0005654 nucleoplasm IDA --
GO:0005737 cytoplasm IDA,IEA 12054534
GO:0005813 colocalizes_with centrosome IDA 20873783
genes like me logo Genes that share ontologies with NEK6: view

Pathways & Interactions for NEK6 Gene

genes like me logo Genes that share pathways with NEK6: view

Pathways by source for NEK6 Gene

SIGNOR curated interactions for NEK6 Gene

Is activated by:

Gene Ontology (GO) - Biological Process for NEK6 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0006468 protein phosphorylation IDA 12840024
GO:0006915 apoptotic process IEA --
GO:0007049 cell cycle IEA --
GO:0007059 chromosome segregation IEA,IDA 14563848
GO:0007077 mitotic nuclear envelope disassembly TAS --
genes like me logo Genes that share ontologies with NEK6: view

Drugs & Compounds for NEK6 Gene

(2) Drugs for NEK6 Gene - From: HMDB

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Magnesium Approved Nutra 0
ATP Investigational Nutra Agonist 0

(1) Additional Compounds for NEK6 Gene - From: HMDB

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
ADP
  • Adenosindiphosphorsaeure
  • Adenosine 5'-pyrophosphate
  • Adenosine diphosphate
  • Adenosine pyrophosphate
  • Adenosine-5'-diphosphate
Full agonist, Agonist 58-64-0
genes like me logo Genes that share compounds with NEK6: view

Transcripts for NEK6 Gene

Unigene Clusters for NEK6 Gene

NIMA-related kinase 6:
Representative Sequences:

Clone Products

  • Addgene plasmids for NEK6

Alternative Splicing Database (ASD) splice patterns (SP) for NEK6 Gene

ExUns: 1 ^ 2 ^ 3 ^ 4a · 4b · 4c ^ 5 ^ 6 ^ 7 ^ 8 ^ 9 ^ 10 ^ 11a · 11b ^ 12 ^ 13 ^ 14 ^ 15 ^ 16a · 16b · 16c
SP1: - - - - - -
SP2: - - - - - - - -
SP3: - - - - - - -
SP4:
SP5: - - - - - -
SP6: -
SP7: - - -
SP8: -

Relevant External Links for NEK6 Gene

GeneLoc Exon Structure for
NEK6
ECgene alternative splicing isoforms for
NEK6

Expression for NEK6 Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and CGAP SAGE for NEK6 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for NEK6 Gene

This gene is overexpressed in Liver (x4.0).

Protein differential expression in normal tissues from HIPED for NEK6 Gene

This gene is overexpressed in Bone (50.2) and Heart (10.8).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MaxQB, and MOPED for NEK6 Gene



NURSA nuclear receptor signaling pathways regulating expression of NEK6 Gene:

NEK6

SOURCE GeneReport for Unigene cluster for NEK6 Gene:

Hs.197071

mRNA Expression by UniProt/SwissProt for NEK6 Gene:

Q9HC98-NEK6_HUMAN
Tissue specificity: Ubiquitous, with highest expression in heart and skeletal muscle. Up-regulated in a variety of malignant cancers, such as breast, colon, lung, and gastric cancers.

Evidence on tissue expression from TISSUES for NEK6 Gene

  • Nervous system(4.5)
  • Kidney(4.3)
  • Pancreas(4.2)
genes like me logo Genes that share expression patterns with NEK6: view

No data available for Protein tissue co-expression partners and Phenotype-based relationships between genes and organs from Gene ORGANizer for NEK6 Gene

Orthologs for NEK6 Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for NEK6 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia NEK6 33 34
  • 99.01 (n)
platypus
(Ornithorhynchus anatinus)
Mammalia NEK6 34
  • 92 (a)
OneToOne
cow
(Bos Taurus)
Mammalia NEK6 33 34
  • 90.63 (n)
dog
(Canis familiaris)
Mammalia NEK6 34 33
  • 89.53 (n)
OneToOne
rat
(Rattus norvegicus)
Mammalia Nek6 33
  • 89.24 (n)
oppossum
(Monodelphis domestica)
Mammalia NEK6 34
  • 89 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Nek6 33 16 34
  • 88.71 (n)
chicken
(Gallus gallus)
Aves NEK6 33 34
  • 75.72 (n)
lizard
(Anolis carolinensis)
Reptilia NEK6 34
  • 73 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia nek6 33
  • 77.44 (n)
Str.12097 33
African clawed frog
(Xenopus laevis)
Amphibia nek6-prov 33
zebrafish
(Danio rerio)
Actinopterygii nek6 33 34
  • 76.31 (n)
worm
(Caenorhabditis elegans)
Secernentea nekl-3 34
  • 66 (a)
OneToMany
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes KIN3 34
  • 23 (a)
OneToMany
sea squirt
(Ciona savignyi)
Ascidiacea -- 34
  • 64 (a)
OneToMany
Species where no ortholog for NEK6 was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • fruit fly (Drosophila melanogaster)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for NEK6 Gene

ENSEMBL:
Gene Tree for NEK6 (if available)
TreeFam:
Gene Tree for NEK6 (if available)

Paralogs for NEK6 Gene

Paralogs for NEK6 Gene

genes like me logo Genes that share paralogs with NEK6: view

Variants for NEK6 Gene

Sequence variations from dbSNP and Humsavar for NEK6 Gene

SNP ID Clin Chr 09 pos Variation AA Info Type
rs1000041159 -- 124,328,842(+) C/A intron_variant
rs1000048696 -- 124,310,774(+) T/G intron_variant
rs1000135275 -- 124,320,663(+) C/G intron_variant
rs1000181137 -- 124,342,482(+) A/C intron_variant
rs1000220895 -- 124,305,012(+) A/C intron_variant

Structural Variations from Database of Genomic Variants (DGV) for NEK6 Gene

Variant ID Type Subtype PubMed ID
dgv12866n54 CNV loss 21841781
esv2677076 CNV deletion 23128226
esv2739034 CNV deletion 23290073
esv2739035 CNV deletion 23290073
esv3573383 CNV loss 25503493
esv3621719 CNV loss 21293372
esv3621720 CNV loss 21293372
nsv1119608 CNV deletion 24896259
nsv471322 CNV loss 18288195
nsv519767 CNV loss 19592680
nsv6711 CNV deletion 18451855
nsv825074 CNV gain 20364138
nsv831719 CNV gain 17160897
nsv831720 CNV loss 17160897

Variation tolerance for NEK6 Gene

Residual Variation Intolerance Score: 24.3% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 3.63; 56.66% of all genes are more intolerant (likely to be disease-causing)

Additional Variant Information for NEK6 Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for
NEK6

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for NEK6 Gene

Disorders for NEK6 Gene

Additional Disease Information for NEK6

Genetic Association Database
(GAD)
Human Genome Epidemiology Navigator
(HuGE)
ATLAS of Genetics and Cytogenetics in Oncology and Haematology

No disorders were found for NEK6 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for NEK6 Gene

Publications for NEK6 Gene

  1. Human NIMA-related kinase 6 is one of the Fe65 WW domain binding proteins. (PMID: 17512906) Lee EJ … Kang SS (Biochemical and biophysical research communications 2007) 3 4 22 58
  2. Interaction of Pin1 with Nek6 and characterization of their expression correlation in Chinese hepatocellular carcinoma patients. (PMID: 16476580) Chen J … Yu L (Biochemical and biophysical research communications 2006) 3 4 22 58
  3. The serine/threonine kinase Nek6 is required for cell cycle progression through mitosis. (PMID: 14563848) Yin MJ … Jallal B (The Journal of biological chemistry 2003) 3 4 22 58
  4. A role for Nek6 kinase activity in preventing senescence? (PMID: 21200151) Fry AM (Cell cycle (Georgetown, Tex.) 2011) 3 4 58
  5. Common genetic variation and performance on standardized cognitive tests. (PMID: 20125193) Cirulli ET … Goldstein DB (European journal of human genetics : EJHG 2010) 3 44 58

Products for NEK6 Gene

  • Addgene plasmids for NEK6

Sources for NEK6 Gene

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