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Aliases for NEK6 Gene

Aliases for NEK6 Gene

  • NIMA Related Kinase 6 2 3
  • NIMA (Never In Mitosis Gene A)-Related Kinase 6 2 3
  • Putative Serine-Threonine Protein Kinase 2 3
  • Never In Mitosis A-Related Kinase 6 3 4
  • NimA-Related Protein Kinase 6 3 4
  • Protein Kinase SID6-1512 3 4
  • NIMA-Related Kinase 6 3 5
  • EC 2.7.11.1 4 63
  • SID6-1512 3

External Ids for NEK6 Gene

Previous GeneCards Identifiers for NEK6 Gene

  • GC09P118134
  • GC09P118666
  • GC09P120473
  • GC09P122395
  • GC09P122396
  • GC09P124099
  • GC09P126059
  • GC09P127019
  • GC09P096633

Summaries for NEK6 Gene

Entrez Gene Summary for NEK6 Gene

  • The protein encoded by this gene is a kinase required for progression through the metaphase portion of mitosis. Inhibition of the encoded protein can lead to apoptosis. This protein also can enhance tumorigenesis by suppressing tumor cell senescence. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]

GeneCards Summary for NEK6 Gene

NEK6 (NIMA Related Kinase 6) is a Protein Coding gene. Among its related pathways are Regulation of Glucokinase by Glucokinase Regulatory Protein and Cell Cycle, Mitotic. GO annotations related to this gene include transferase activity, transferring phosphorus-containing groups and protein tyrosine kinase activity. An important paralog of this gene is NEK2.

UniProtKB/Swiss-Prot for NEK6 Gene

  • Protein kinase which plays an important role in mitotic cell cycle progression. Required for chromosome segregation at metaphase-anaphase transition, robust mitotic spindle formation and cytokinesis. Phosphorylates ATF4, CIR1, PTN, RAD26L, RBBP6, RPS7, RPS6KB1, TRIP4, STAT3 and histones H1 and H3. Phosphorylates KIF11 to promote mitotic spindle formation. Involved in G2/M phase cell cycle arrest induced by DNA damage. Inhibition of activity results in apoptosis. May contribute to tumorigenesis by suppressing p53/TP53-induced cancer cell senescence.

Gene Wiki entry for NEK6 Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for NEK6 Gene

Genomics for NEK6 Gene

Regulatory Elements for NEK6 Gene

Enhancers for NEK6 Gene
GeneHancer Identifier Score Enhancer Sources TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor Binding Sites within enhancer Other Gene Targets for Enhancer

Enhancers around NEK6 on UCSC Golden Path with GeneCards custom track

Promoters for NEK6 Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters

ENSRs around NEK6 on UCSC Golden Path with GeneCards custom track

Genomic Location for NEK6 Gene

Chromosome:
9
Start:
124,257,606 bp from pter
End:
124,378,316 bp from pter
Size:
120,711 bases
Orientation:
Plus strand

Genomic View for NEK6 Gene

Genes around NEK6 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
NEK6 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for NEK6 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for NEK6 Gene

Proteins for NEK6 Gene

  • Protein details for NEK6 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q9HC98-NEK6_HUMAN
    Recommended name:
    Serine/threonine-protein kinase Nek6
    Protein Accession:
    Q9HC98
    Secondary Accessions:
    • B7Z2D9
    • Q5TBG3
    • Q5TBG9
    • Q6FG86
    • Q6IAR3
    • Q96E83
    • Q9ULX2

    Protein attributes for NEK6 Gene

    Size:
    313 amino acids
    Molecular mass:
    35714 Da
    Cofactor:
    Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
    Quaternary structure:
    • Interacts with STAT3 and RPS6KB1 (By similarity). Interacts with NEK9, predominantly in mitosis. Interacts with KIF11 (via C-terminus). Interacts with APBB1 (via WW domain). Interacts with ANKRA2, ATF4, ARHGAP33, CDC42, CIR1, PRAM1, PTN, PRDX3, PIN1, RAD26L, RBBP6, RPS7 and TRIP4.
    SequenceCaution:
    • Sequence=AAG13417.1; Type=Miscellaneous discrepancy; Note=Contaminating sequence. Sequence of unknown origin in the N-terminal part.; Evidence={ECO:0000305}; Sequence=AAH00101.2; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305}; Sequence=AAH04174.2; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305}; Sequence=AAH04209.2; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305}; Sequence=BAA85045.1; Type=Erroneous initiation; Note=Translation N-terminally extended.; Evidence={ECO:0000305};

    Alternative splice isoforms for NEK6 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for NEK6 Gene

Proteomics data for NEK6 Gene at MOPED

Post-translational modifications for NEK6 Gene

  • Autophosphorylated. Phosphorylation at Ser-206 is required for its activation. Phosphorylated upon IR or UV-induced DNA damage. Phosphorylated by CHEK1 and CHEK2. Interaction with APBB1 down-regulates phosphorylation at Thr-210.
  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

Domains & Families for NEK6 Gene

Gene Families for NEK6 Gene

Suggested Antigen Peptide Sequences for NEK6 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

Q9HC98

UniProtKB/Swiss-Prot:

NEK6_HUMAN :
  • Displays an autoinhibited conformation: Tyr-108 side chain points into the active site, interacts with the activation loop, and blocks the alphaC helix. The autoinhibitory conformation is released upon binding with NEK9.
  • Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
Domain:
  • Displays an autoinhibited conformation: Tyr-108 side chain points into the active site, interacts with the activation loop, and blocks the alphaC helix. The autoinhibitory conformation is released upon binding with NEK9.
  • Contains 1 protein kinase domain.
Family:
  • Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
genes like me logo Genes that share domains with NEK6: view

Function for NEK6 Gene

Molecular function for NEK6 Gene

UniProtKB/Swiss-Prot CatalyticActivity:
ATP + a protein = ADP + a phosphoprotein.
UniProtKB/Swiss-Prot EnzymeRegulation:
Binding to NEK9 stimulates its activity by releasing the autoinhibitory function of Tyr-108.
UniProtKB/Swiss-Prot Function:
Protein kinase which plays an important role in mitotic cell cycle progression. Required for chromosome segregation at metaphase-anaphase transition, robust mitotic spindle formation and cytokinesis. Phosphorylates ATF4, CIR1, PTN, RAD26L, RBBP6, RPS7, RPS6KB1, TRIP4, STAT3 and histones H1 and H3. Phosphorylates KIF11 to promote mitotic spindle formation. Involved in G2/M phase cell cycle arrest induced by DNA damage. Inhibition of activity results in apoptosis. May contribute to tumorigenesis by suppressing p53/TP53-induced cancer cell senescence.
UniProtKB/Swiss-Prot Induction:
Up-regulated during the M phase of cell cycle progression. Down-regulated in both replicative and premature senescence of cancer cells.

Enzyme Numbers (IUBMB) for NEK6 Gene

Gene Ontology (GO) - Molecular Function for NEK6 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005524 ATP binding IEA,IDA 14563848
genes like me logo Genes that share ontologies with NEK6: view
genes like me logo Genes that share phenotypes with NEK6: view

Animal Models for NEK6 Gene

MGI Knock Outs for NEK6:

Animal Model Products

Flow Cytometry Products

No data available for Human Phenotype Ontology , Transcription Factor Targets and HOMER Transcription for NEK6 Gene

Localization for NEK6 Gene

Subcellular locations from UniProtKB/Swiss-Prot for NEK6 Gene

Cytoplasm. Nucleus. Nucleus speckle. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole. Note=Colocalizes with APBB1 at the nuclear speckles. Colocalizes with PIN1 in the nucleus. Colocalizes with ATF4, CIR1, ARHGAP33, ANKRA2, CDC42, NEK9, RAD26L, RBBP6, RPS7, TRIP4, RELB and PHF1 in the centrosome. Localizes to spindle microtubules in metaphase and anaphase and to the midbody during cytokinesis.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for NEK6 Gene COMPARTMENTS Subcellular localization image for NEK6 gene
Compartment Confidence
cytoskeleton 5
cytosol 5
nucleus 5

Gene Ontology (GO) - Cellular Components for NEK6 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005654 nucleoplasm IDA --
GO:0043231 intracellular membrane-bounded organelle IDA --
genes like me logo Genes that share ontologies with NEK6: view

Pathways & Interactions for NEK6 Gene

genes like me logo Genes that share pathways with NEK6: view

Pathways by source for NEK6 Gene

SIGNOR curated interactions for NEK6 Gene

Is activated by:

Gene Ontology (GO) - Biological Process for NEK6 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0007067 mitotic nuclear division IEA --
GO:0007165 signal transduction IMP 12761501
GO:0018105 peptidyl-serine phosphorylation IDA 19001501
GO:0031572 G2 DNA damage checkpoint IMP 18728393
GO:0046777 protein autophosphorylation IDA 14563848
genes like me logo Genes that share ontologies with NEK6: view

Drugs & Compounds for NEK6 Gene

(2) Drugs for NEK6 Gene - From: HMDB

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Adenosine triphosphate Approved Nutra 0
Magnesium Nutra 0

(1) Additional Compounds for NEK6 Gene - From: HMDB

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
ADP
  • Adenosindiphosphorsaeure
  • Adenosine 5'-pyrophosphate
  • Adenosine diphosphate
  • Adenosine pyrophosphate
  • Adenosine-5'-diphosphate
Full agonist, Agonist 58-64-0
genes like me logo Genes that share compounds with NEK6: view

Transcripts for NEK6 Gene

Unigene Clusters for NEK6 Gene

NIMA-related kinase 6:
Representative Sequences:

Alternative Splicing Database (ASD) splice patterns (SP) for NEK6 Gene

ExUns: 1 ^ 2 ^ 3 ^ 4a · 4b · 4c ^ 5 ^ 6 ^ 7 ^ 8 ^ 9 ^ 10 ^ 11a · 11b ^ 12 ^ 13 ^ 14 ^ 15 ^ 16a · 16b · 16c
SP1: - - - - - -
SP2: - - - - - - - -
SP3: - - - - - - -
SP4:
SP5: - - - - - -
SP6: -
SP7: - - -
SP8: -

Relevant External Links for NEK6 Gene

GeneLoc Exon Structure for
NEK6
ECgene alternative splicing isoforms for
NEK6

Expression for NEK6 Gene

mRNA expression in normal human tissues for NEK6 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for NEK6 Gene

This gene is overexpressed in Liver (x4.0).

Protein differential expression in normal tissues from HIPED for NEK6 Gene

This gene is overexpressed in Bone (50.2) and Heart (10.8).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MOPED, and MaxQB for NEK6 Gene



SOURCE GeneReport for Unigene cluster for NEK6 Gene Hs.197071

mRNA Expression by UniProt/SwissProt for NEK6 Gene

Q9HC98-NEK6_HUMAN
Tissue specificity: Ubiquitous, with highest expression in heart and skeletal muscle. Up-regulated in a variety of malignant cancers, such as breast, colon, lung, and gastric cancers.
genes like me logo Genes that share expression patterns with NEK6: view

Primer Products

No data available for Protein tissue co-expression partners for NEK6 Gene

Orthologs for NEK6 Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for NEK6 Gene

Organism Taxonomy Gene Similarity Type Details
cow
(Bos Taurus)
Mammalia NEK6 35
  • 90.63 (n)
  • 96.17 (a)
NEK6 36
  • 96 (a)
OneToOne
dog
(Canis familiaris)
Mammalia NEK6 35
  • 89.53 (n)
  • 96.04 (a)
NEK6 36
  • 87 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Nek6 35
  • 88.71 (n)
  • 95.21 (a)
Nek6 16
Nek6 36
  • 95 (a)
OneToOne
chimpanzee
(Pan troglodytes)
Mammalia NEK6 35
  • 99.01 (n)
  • 99.11 (a)
NEK6 36
  • 99 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Nek6 35
  • 89.24 (n)
  • 96.17 (a)
oppossum
(Monodelphis domestica)
Mammalia NEK6 36
  • 89 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia NEK6 36
  • 92 (a)
OneToOne
chicken
(Gallus gallus)
Aves NEK6 35
  • 75.72 (n)
  • 88.82 (a)
NEK6 36
  • 89 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia NEK6 36
  • 73 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia nek6 35
  • 77.44 (n)
  • 88.89 (a)
Str.12097 35
African clawed frog
(Xenopus laevis)
Amphibia nek6-prov 35
zebrafish
(Danio rerio)
Actinopterygii nek6 35
  • 76.31 (n)
  • 83.52 (a)
nek6 36
  • 75 (a)
OneToOne
worm
(Caenorhabditis elegans)
Secernentea nekl-3 36
  • 66 (a)
OneToMany
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes KIN3 36
  • 23 (a)
OneToMany
sea squirt
(Ciona savignyi)
Ascidiacea -- 36
  • 64 (a)
OneToMany
Species with no ortholog for NEK6:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • fruit fly (Drosophila melanogaster)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for NEK6 Gene

ENSEMBL:
Gene Tree for NEK6 (if available)
TreeFam:
Gene Tree for NEK6 (if available)

Paralogs for NEK6 Gene

Paralogs for NEK6 Gene

genes like me logo Genes that share paralogs with NEK6: view

Variants for NEK6 Gene

Sequence variations from dbSNP and Humsavar for NEK6 Gene

SNP ID Clin Chr 09 pos Sequence Context AA Info Type
rs748659 -- 124,333,424(-) TGACC(C/T)AGGGG intron-variant
rs748660 -- 124,333,370(-) TCACT(C/G)GGGGG intron-variant
rs748661 -- 124,333,295(-) TGTGA(C/T)GGCAA intron-variant
rs748741 -- 124,327,496(-) CTGCA(C/G/T)GGTCA intron-variant
rs749355 -- 124,327,843(+) CTGGC(A/G)GTTTT intron-variant

Structural Variations from Database of Genomic Variants (DGV) for NEK6 Gene

Variant ID Type Subtype PubMed ID
nsv831719 CNV Gain 17160897
nsv831720 CNV Loss 17160897
nsv6711 CNV Loss 18451855
nsv471322 CNV Loss 18288195
nsv519767 CNV Loss 19592680
nsv825074 CNV Gain 20364138
nsv893833 CNV Gain 21882294
esv2677076 CNV Deletion 23128226
esv2739034 CNV Deletion 23290073
esv2739035 CNV Deletion 23290073

Variation tolerance for NEK6 Gene

Residual Variation Intolerance Score: 24.3% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 3.63; 56.66% of all genes are more intolerant (likely to be disease-causing)

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot and Relevant External Links for NEK6 Gene

Disorders for NEK6 Gene

Relevant External Links for NEK6

Genetic Association Database (GAD)
NEK6
Human Genome Epidemiology (HuGE) Navigator
NEK6
Atlas of Genetics and Cytogenetics in Oncology and Haematology:
NEK6

No disorders were found for NEK6 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for NEK6 Gene

Publications for NEK6 Gene

  1. Human NIMA-related kinase 6 is one of the Fe65 WW domain binding proteins. (PMID: 17512906) Lee E.J. … Kang S.S. (Biochem. Biophys. Res. Commun. 2007) 3 4 23 67
  2. Interaction of Pin1 with Nek6 and characterization of their expression correlation in Chinese hepatocellular carcinoma patients. (PMID: 16476580) Chen J. … Yu L. (Biochem. Biophys. Res. Commun. 2006) 3 23
  3. The serine/threonine kinase Nek6 is required for cell cycle progression through mitosis. (PMID: 14563848) Yin M.J. … Jallal B. (J. Biol. Chem. 2003) 3 23
  4. Molecular basis for the substrate specificity of NIMA-related kinase-6 (NEK6). Evidence that NEK6 does not phosphorylate the hydrophobic motif of ribosomal S6 protein kinase and serum- and glucocorticoid-induced protein kinase in vivo. (PMID: 12023960) Lizcano J.M. … Alessi D.R. (J. Biol. Chem. 2002) 3 23
  5. Identification and assignment of the human NIMA-related protein kinase 7 gene (NEK7) to human chromosome 1q31.3. (PMID: 11701951) Kimura M. … Okano Y. (Cytogenet. Cell Genet. 2001) 3 23

Products for NEK6 Gene

Sources for NEK6 Gene

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