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NEK2 Gene

protein-coding   GIFtS: 70
GCID: GC01M211836

NIMA-Related Kinase 2

(Previous name: NIMA (never in mitosis gene a)-related kinase 2)
  See related diseases
at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
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Aliases
NIMA-Related Kinase 21 2     EC 2.7.11.13 8
NIMA (Never In Mitosis Gene A)-Related Kinase 21 2     RP672 5
HSPK 211 3     HsPK212
NimA-Like Protein Kinase 12 3     Serine/Threonine-Protein Kinase Nek22
NimA-Related Protein Kinase 22 3     Never In Mitosis A-Related Kinase 23
NEK2A2 3     EC 2.7.118
NLK12 3     

External Ids:    HGNC: 77451   Entrez Gene: 47512   Ensembl: ENSG000001176507   OMIM: 6040435   UniProtKB: P519553   

Export aliases for NEK2 gene to outside databases

Previous GC identifers: GC01M210565 GC01M207702 GC01M208475 GC01M208915 GC01M208224 GC01M209902 GC01M182513


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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Entrez Gene summary for NEK2 Gene:
This gene encodes a serine/threonine-protein kinase that is involved in mitotic regulation. This protein is
localized to the centrosome, and undetectable during G1 phase, but accumulates progressively throughout the S
phase, reaching maximal levels in late G2 phase. Alternatively spliced transcript variants encoding different
isoforms with distinct C-termini have been noted for this gene. (provided by RefSeq, Feb 2011)

GeneCards Summary for NEK2 Gene:
NEK2 (NIMA-related kinase 2) is a protein-coding gene. Diseases associated with NEK2 include retinitis pigmentosa 67, and van der woude syndrome. GO annotations related to this gene include protein phosphatase binding and protein serine/threonine kinase activity. An important paralog of this gene is NEK4.

UniProtKB/Swiss-Prot: NEK2_HUMAN, P51955
Function: Protein kinase which is involved in the control of centrosome separation and bipolar spindle formation
in mitotic cells and chromatin condensation in meiotic cells. Regulates centrosome separation (essential for the
formation of bipolar spindles and high-fidelity chromosome separation) by phosphorylating centrosomal proteins
such as CROCC, CEP250 and NINL, resulting in their displacement from the centrosomes. Regulates kinetochore
microtubule attachment stability in mitosis via phosphorylation of NDC80. Involved in regulation of mitotic
checkpoint protein complex via phosphorylation of CDC20 and MAD2L1. Plays an active role in chromatin
condensation during the first meiotic division through phosphorylation of HMGA2. Phosphorylates: PPP1CC; SGOL1;
NECAB3 and NPM1. Essential for localization of MAD2L1 to kinetochore and MAPK1 and NPM1 to the centrosome.
Isoform 1 phosphorylates and activates NEK11 in G1/S-arrested cells. Isoform 2, which is not present in the
nucleolus, does not

Gene Wiki entry for NEK2 Gene


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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RefSeq DNA sequence:
NC_000001.11  NT_004487.20  NC_018912.2  
Regulatory elements:
   Regulatory transcription factor binding sites in the NEK2 gene promoter:
         Bach1   C/EBPbeta   Bach2   E47   MEF-2A   CBF-A   Hand1   Cart-1   aMEF-2   CBF(2)   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidNEK2 promoter sequence
   Search Chromatin IP Primers for NEK2

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat NEK2


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 1q32.3   Ensembl cytogenetic band:  1q32.3   HGNC cytogenetic band: 1q32.3

NEK2 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
NEK2 gene location

GeneLoc information about chromosome 1         GeneLoc Exon Structure

GeneLoc location for GC01M211836:  view genomic region     (about GC identifiers)

Start:
211,831,598 bp from pter      End:
211,848,972 bp from pter
Size:
17,375 bases      Orientation:
minus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Cloud-Clone Corp.,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Cloud-Clone Corp., Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, and/or Cloud-Clone Corp.)
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UniProtKB/Swiss-Prot: NEK2_HUMAN, P51955 (See protein sequence)
Recommended Name: Serine/threonine-protein kinase Nek2  
Size: 445 amino acids; 51763 Da
Cofactor: Magnesium
Subunit: Isoform 1, isoform 2 and isoform 4 form homo- and heterodimers. Interacts with NECAB3 and HMGA2 (By
similarity). Isoform 1 interacts with CDC20, CTNB1, MAD1L1, MAPK, NEK11, NPM1, NDC80, PCNT and SGOL1. Isoform 1
interacts with STK3/MST2 (via SARAH domain) and SAV1 (via SARAH domain). Isoform 1 and isoform 2 interact with
MAD2L1. Isoform 1 and isoform 4 interact with PPP1CA and PPP1CC
Sequence caution: Sequence=AAH65932.1; Type=Frameshift; Positions=213;
Selected PDB 3D structures from and Proteopedia for NEK2 (see all 20):
2JAV (3D)        2W5A (3D)        2W5B (3D)        2W5H (3D)        2WQO (3D)        2XK3 (3D)    
Secondary accessions: Q53FD6 Q5I1Z9 Q5VXZ1 Q6NZX8 Q7Z634 Q86XH2 Q96QN9
Alternative splicing: 4 isoforms:  P51955-1   P51955-2   P51955-3   P51955-4   

Explore the universe of human proteins at neXtProt for NEK2: NX_P51955

Explore proteomics data for NEK2 at MOPED

Post-translational modifications: 

  • Activated by autophosphorylation. Protein phosphatase 1 represses autophosphorylation and activation of isoform 1
    by dephosphorylation. Phosphorylation by STK3/MST2 is necessary for its localization to the centrosome1
  • Ubiquitination2 at Lys37, Lys152, Lys174, Lys359, Lys362
  • Modification sites at PhosphoSitePlus
  • 4 DME Specific Peptides for NEK2 (P51955)
     VLHRDLK  LGDFGLA  KSDIWSLGC  GTPYYMSPE 


    See NEK2 Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins (3 alternative transcripts): 
    NP_001191111.1  NP_001191112.1  NP_002488.1  

    ENSEMBL proteins: 
     ENSP00000355966   ENSP00000355965   ENSP00000440237  
    Reactome Protein details: P51955

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    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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    IUPHAR Guide to PHARMACOLOGY protein family classification: NIMA-related kinase 2
    NIMA (never in mitosis gene a)- related kinase (NEK) family

    4 InterPro protein domains:
     IPR002290 Ser/Thr_dual-sp_kinase_dom
     IPR011009 Kinase-like_dom
     IPR008271 Ser/Thr_kinase_AS
     IPR000719 Prot_kinase_dom

    Graphical View of Domain Structure for InterPro Entry P51955

    ProtoNet protein and cluster: P51955

    UniProtKB/Swiss-Prot: NEK2_HUMAN, P51955
    Domain: The leucine-zipper domain is required for its dimerization and activation
    Similarity: Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily
    Similarity: Contains 1 protein kinase domain


    NEK2 for domains           About GeneDecksing


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Molecular Function:

         UniProtKB/Swiss-Prot Summary: NEK2_HUMAN, P51955
    Function: Protein kinase which is involved in the control of centrosome separation and bipolar spindle formation
    in mitotic cells and chromatin condensation in meiotic cells. Regulates centrosome separation (essential for the
    formation of bipolar spindles and high-fidelity chromosome separation) by phosphorylating centrosomal proteins
    such as CROCC, CEP250 and NINL, resulting in their displacement from the centrosomes. Regulates kinetochore
    microtubule attachment stability in mitosis via phosphorylation of NDC80. Involved in regulation of mitotic
    checkpoint protein complex via phosphorylation of CDC20 and MAD2L1. Plays an active role in chromatin
    condensation during the first meiotic division through phosphorylation of HMGA2. Phosphorylates: PPP1CC; SGOL1;
    NECAB3 and NPM1. Essential for localization of MAD2L1 to kinetochore and MAPK1 and NPM1 to the centrosome.
    Isoform 1 phosphorylates and activates NEK11 in G1/S-arrested cells. Isoform 2, which is not present in the
    nucleolus, does not
    Catalytic activity: ATP + a protein = ADP + a phosphoprotein
    Enzyme regulation: Isoform 1 is inhibited by ionizing radiation in the presence of PPP1CA. Its catalytic activity
    is inhibited by the inhibitor CCT241950. In the presence of this inhibitor, displays an autoinhibited
    conformation: Tyr-70 side chain points into the active site, interacts with the activation loop, and blocks the
    alphaC helix
    Induction: Expression and activity peak in the G2 phase of the mitotic cycle and decrease once the cells have
    entered mitosis due to degradation by the anaphase promoting complex APC/C-CDC20. In G1 phase, both isoform 1 and
    isoform 2 are almost undetectable. However, at the G1/S transition, there is an increase in expression of both
    isoforms which then remain at this increased level throughout S and G2. At the onset of mitosis, isoform 1
    undergoes a rapid disappearance whereas isoform 2 continues to be present at about the same level as in G2.
    During the rest of mitosis, isoform 1 remains absent, while isoform 2 only begins to decline upon re-entry into
    the next G1 phase

         Genatlas biochemistry entry for NEK2:
    never in mitosis gene A-related kinase 2,Aspergillus nidulans NIMA ortholog,located to the centrosome,expressed in
    a cell-cycle dependent pattern,undetectable during G1,appearing in S,reacting maximum levels in late G2

         Enzyme Numbers (IUBMB): EC 2.7.11.11 2 EC 2.7.112

         Gene Ontology (GO): Selected molecular function terms (see all 8):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0004672protein kinase activity IDA10880350
    GO:0004674protein serine/threonine kinase activity TAS16280549
    GO:0004713protein tyrosine kinase activity ----
    GO:0005515protein binding IPI10880350
    GO:0005524ATP binding IEA--
         
    NEK2 for ontologies           About GeneDecksing


    Phenotypes:
         3 GenomeRNAi human phenotypes for NEK2:
     Decreased substrate adherent c  Decreased telomerase activity  Increased G1 DNA content 

    Animal Models:
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    miRNA
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    7 qRT-PCR Assays for microRNAs that regulate NEK2:
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    SwitchGear 3'UTR luciferase reporter plasmidNEK2 3' UTR sequence
    Inhib. RNA
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    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Subcellular locations from UniProtKB/Swiss-Prot
    NEK2_HUMAN, P51955: Isoform 1: Nucleus. Nucleus, nucleolus. Cytoplasm. Cytoplasm, cytoskeleton, microtubule
    organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole. Chromosome, centromere, kinetochore.
    Chromosome, centromere (By similarity). Note=STK3/MST2 and SAV1 are required for its targeting to the centrosome.
    Colocalizes with SGOL1 and MAD1L1 at the kinetochore. Not associated with kinetochore in the interphase but
    becomes associated with it upon the breakdown of the nuclear envelope. Has a nucleolar targeting/ retention
    activity via a coiled-coil domain at the C-terminal end
    NEK2_HUMAN, P51955: Isoform 2: Cytoplasm. Note=Predominantly cytoplasmic
    NEK2_HUMAN, P51955: Isoform 4: Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
    Note=Predominantly nuclear
    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    cytoskeleton5
    nucleus5
    cytosol4
    peroxisome1

    Gene Ontology (GO): Selected cellular component terms (see all 13):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000776colocalizes with kinetochore IDA17621308
    GO:0000777condensed chromosome kinetochore IEA--
    GO:0000794condensed nuclear chromosome IEA--
    GO:0000922spindle pole IEA--
    GO:0005634nucleus IDA--

    NEK2 for ontologies           About GeneDecksing


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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    SuperPaths for NEK2 About                                                                                                See pathways by source

    SuperPathContained pathways About
    1Regulation of PLK1 Activity at G2/M Transition
    Recruitment of mitotic centrosome proteins and complexes0.86
    Regulation of PLK1 Activity at G2/M Transition0.77
    Loss of Nlp from mitotic centrosomes0.86
    G2/M Transition0.70
    Centrosome maturation0.86
    Mitotic G2-G2/M phases0.69
    Loss of proteins required for interphase microtubule organizationfrom the centrosome0.86
    2Cell Cycle, Mitotic
    Cell Cycle, Mitotic0.90
    Cell Cycle0.90
    3FOXM1 transcription factor network
    FOXM1 transcription factor network
    4Cell cycle Spindle assembly and chromosome separation
    Cell cycle Spindle assembly and chromosome separation
    5Cell cycle Role of APC in cell cycle regulation
    Cell cycle Role of APC in cell cycle regulation

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways


    2 GeneGo (Thomson Reuters) Pathways for NEK2
        Cell cycle Role of APC in cell cycle regulation
    Cell cycle Spindle assembly and chromosome separation

    1 BioSystems Pathway for NEK2
        FOXM1 transcription factor network

    4 Reactome Pathways for NEK2
        Recruitment of mitotic centrosome proteins and complexes
    Loss of proteins required for interphase microtubule organization,from the centrosome
    Regulation of PLK1 Activity at G2/M Transition
    Loss of Nlp from mitotic centrosomes



    NEK2 for pathways           About GeneDecksing

        Custom Pathway & Disease-focused RT2 Profiler PCR Arrays for NEK2
    Interactions:

        GeneGlobe Interaction Network for NEK2

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    Selected Interacting proteins for NEK2 (P519551, 2, 3 ENSP000003559664) via UniProtKB, MINT, STRING, and/or I2D (see all 178)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    DCTN1Q142032, 3, ENSP000003547914MINT-8263654 I2D: score=2 STRING: ENSP00000354791
    EDRF1Q3B7T12, 3, ENSP000003367274MINT-8263621 I2D: score=2 STRING: ENSP00000336727
    PDE4DIPQ5VU432, 3, ENSP000003583604MINT-8263731 I2D: score=2 STRING: ENSP00000358360
    CCDC85AQ96PX62, 3MINT-8263632 I2D: score=2 
    CDC27P302601, 3EBI-633182,EBI-994813 I2D: score=2 
    About this table

    Gene Ontology (GO): Selected biological process terms (see all 16):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0000070mitotic sister chromatid segregation IEA--
    GO:0000086G2/M transition of mitotic cell cycle TAS--
    GO:0000278mitotic cell cycle TAS--
    GO:0001824blastocyst development IEA--
    GO:0006468protein phosphorylation IDA--

    NEK2 for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
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    Browse Small Molecules at EMD Millipore
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    Browse Tocris compounds for NEK2

    3 HMDB Compounds for NEK2    About this table
    CompoundSynonyms CAS #PubMed Ids
    ADPadenosindiphosphorsaeure (see all 8)58-64-0--
    Adenosine triphosphate5'-(tetrahydrogen triphosphate) Adenosine (see all 24)56-65-5--
    MagnesiumMagnesium (see all 2)7439-95-4--

    1 DrugBank Compound for NEK2    About this table
    CompoundSynonyms CAS #TypeActionsPubMed Ids
    5-[(Z)-(5-CHLORO-2-OXO-1,2-DIHYDRO-3H-INDOL-3-YLIDENE)METHYL]-N-(DIETHYLAMINO)ETHYL]-2,4-DIMETHYL-1H-PYRROLE-3-CARBOXAMIDE-- --target--10592235

    2 Novoseek inferred chemical compound relationships for NEK2 gene    About this table
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    threonine 52.6 8 18095313 (2), 15387139 (1), 20034488 (1), 16084011 (1) (see all 5)
    serine 46 11 18095313 (2), 12386167 (2), 15387139 (1), 16084011 (1) (see all 7)



    NEK2 for compounds           About GeneDecksing



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    Conferences by KenesGroup, exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, and/or QIAGEN )
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    REFSEQ mRNAs for NEK2 gene (3 alternative transcripts): 
    NM_001204182.1  NM_001204183.1  NM_002497.3  

    Unigene Cluster for NEK2:

    NIMA-related kinase 2
    Hs.153704  [show with all ESTs]
    Unigene Representative Sequence: BC043502
    5 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000366999(uc021pir.1 uc021piq.1 uc001hir.2 uc001hit.2)
    ENST00000462283 ENST00000489633 ENST00000366998(uc001his.4) ENST00000540251

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    Additional mRNA sequence: 

    AK223353.1 AK225522.1 AK303092.1 AY045701.1 AY863109.1 BC043502.1 BC052807.1 BC065932.1 
    BT019729.1 DQ786247.1 DQ786281.1 U11050.1 Z25425.1 Z29066.1 

    8 DOTS entries:

    DT.114738  DT.100783245  DT.100783250  DT.95275694  DT.100009881  DT.100783248  DT.95275695  DT.99990184 

    Selected AceView cDNA sequences (see all 133):

    BC065932 AI074569 BI223141 BG771044 BQ227186 BC043502 CR601480 AU131125 
    AI572577 CR593900 BU517064 BQ431771 BM800724 AA682321 CB163013 AL516673 
    BC052807 BX368912 AI470415 D80285 NM_002497 BM853783 BU622277 Z29066 

    GeneLoc Exon Structure

    Selected Alternative Splicing Database (ASD) splice patterns (SP) for NEK2 (see all 7)    About this scheme

    ExUns: 1 ^ 2 ^ 3a · 3b ^ 4 ^ 5a · 5b · 5c ^ 6a · 6b ^ 7a · 7b · 7c · 7d · 7e ^ 8a · 8b ^ 9a · 9b · 9c · 9d · 9e
    SP1:                          -                 -                 -                                                                     
    SP2:                          -                 -     -           -                                                                     
    SP3:                          -                 -                 -                                   -                                 
    SP4:                                -     -     -     -           -                                   -     -                           
    SP5:                                                  -           -                                   -     -                           


    ECgene alternative splicing isoforms for NEK2

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

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    NEK2 expression in normal human tissues (normalized intensities)      NEK2 embryonic expression: see
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: --
    NEK2 Expression
    About this image


    NEK2 expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database
     selected tissues (see all 2) fully expand
     
     Blood (Hematopoietic System)
             Proerythroblasts Hematopoietic Bone Marrow
     
     Testis (Reproductive System)
    NEK2 Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    NEK2 Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.153704

    UniProtKB/Swiss-Prot: NEK2_HUMAN, P51955
    Tissue specificity: Isoform 1 and isoform 2 are expressed in peripheral blood T-cells and a wide variety of
    transformed cell types. Isoform 1 and isoform 4 are expressed in the testis. Up-regulated in various cancer cell
    lines, as well as primary breast tumors

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    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
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    This gene was present in the common ancestor of animals and fungi.

    Orthologs for NEK2 gene from Selected species (see all 18)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Nek21 , 5 NIMA (never in mitosis gene a)-related expressed kinase more1, 5 83.3(n)1
    88.49(a)1
      1 (96.94 cM)5
    180051  NM_010892.31  NP_035022.21 
     1918214445 
    chicken
    (Gallus gallus)
    Aves NEK21 NIMA (never in mitosis gene a)-related kinase 2 71.63(n)
    77.43(a)
      421376  NM_001031050.2  NP_001026221.1 
    lizard
    (Anolis carolinensis)
    Reptilia NEK26
    NIMA-related kinase 2
    78(a)
    1 ↔ 1
    1(238093862-238109849)
    African clawed frog
    (Xenopus laevis)
    Amphibia MGC532022 similar to NIMA (never in mitosis gene a)-related kinase more 74.16(n)    AB019556.1 
    zebrafish
    (Danio rerio)
    Actinopterygii zgc556022 similar to NIMA (never in mitosis gene a)-related kinase more 75.99(n)   394025  BC048055.1 
    fruit fly
    (Drosophila melanogaster)
    Insecta Nek23 mitosis protein serine/threonine kinase 48(a)   7C1   --
    baker's yeast
    (Saccharomyces cerevisiae)
    Saccharomycetes KIN3(YAR018C)4 Nonessential serine/threonine protein kinase; possible more   --   1(171703-170396) 851273  NP_009410.1 


    ENSEMBL Gene Tree for NEK2 (if available)
    TreeFam Gene Tree for NEK2 (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
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    Paralogs for NEK2 gene
    NEK42  NEK62  NEK12  NEK112  NEK32  NEK72  NEK52  NEK82  
    NEK102  NEK92  
    Selected SIMAP similar genes for NEK2 using alignment to 2 protein entries:     NEK2_HUMAN (see all proteins) (see all similar genes):
    NEK6    NEK7    NEK5    MARK4    DKFZp564L2416    NEK8
    PRKAA1    NEK3    MST094    NEK9    RPS6KA2    NEK1
    NUAK1    DKFZp434B079    PAK4    STK11    CDK4    NEK11

    NEK2 for paralogs           About GeneDecksing


    4 Pseudogenes.org Pseudogenes for NEK2
    PGOHUM00000247698 PGOHUM00000248029 PGOHUM00000246368 PGOHUM00000240306


    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
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    Selected SNPs for NEK2 (see all 409)    About this table    
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 1 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs601963121,2
    C--211832568(+) AAAAA-/AGATAA 1 -- int12Minor allele frequency- A:0.25NA CSA 4
    rs13732921,2
    C,F,O,A,H--211833926(-) AGGCAC/TACACC 1 -- int126Minor allele frequency- T:0.16MN NA NS EA WA CSA 2732
    rs18225691,2
    C,F,A,H--211834028(-) TTCAAG/CGAAAA 1 -- int125Minor allele frequency- C:0.16NA NS EA WA CSA 2570
    rs354519321,2
    C--211836192(+) AAAAA-/AGAATA 2 -- ut31 int11Minor allele frequency- A:0.50NA 2
    rs107104841,2
    C--211837405(+) ttttt-/TAAATA 2 -- int1 trp31Minor allele frequency- T:0.00NA 2
    rs113815141,2
    C--211843194(+) CTCTGC/-TTTTT 3 -- int12Minor allele frequency- -:0.25NA CSA 4
    rs725077881,2
    C--211843194(+) CTCTG-/CGTTTT 3 -- int10--------
    rs358610121,2
    C--211843195(+) TCTGT-/CTTTTA 3 -- int10--------
    rs717217811,2
    C--211845957(+) AAAAA-/A/AA  
            
    CAGAG
    3 -- int10--------
    rs359801531,2
    C--211849197(+) ATAGA-/GATCGCC 3 -- us2k11Minor allele frequency- GA:0.00CSA 2

    HapMap Linkage Disequilibrium report for NEK2 (211831598 - 211848972 bp)

    Structural Variations
          Database of Genomic Variants (DGV) variations for NEK2: --
    Human Gene Mutation Database (HGMD): NEK2
    Site Specific Mutation Identification with PCR Assays
    1 Copy Number PCR Panel containing NEK2:
    Birth Defects
    SeqTarget long-range PCR primers for resequencing NEK2
    DNA2.0 Custom Variant and Variant Library Synthesis for NEK2

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Conferences by KenesGroup, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

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    OMIM gene information: 604043   
    OMIM disorders: 615565  
    9 diseases for NEK2:    About MalaCards
    retinitis pigmentosa 67    van der woude syndrome    polyploidy    leber congenital amaurosis
    seminoma    male infertility    infertility    neuroblastoma
    breast cancer


    NEK2 for disorders           About GeneDecksing

    3 Novoseek inferred disease relationships for NEK2 gene    About this table

    Disease   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    cancer 16.4 7 16084011 (2), 19124027 (1), 17197699 (1), 20034488 (1)
    breast cancer 0 1 16854412 (1)
    tumors 0 2 15492258 (1), 16854412 (1)

    Genetic Association Database (GAD): NEK2
    Human Genome Epidemiology (HuGE) Navigator: NEK2 (5 documents)

    Export disorders for NEK2 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
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    PubMed articles for NEK2 gene, integrated from 10 sources (see all 117):
    (articles sorted by number of sources associating them with NEK2)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Identification of 21 novel human protein kinases, including 3 members of a family related to the cell cycle regulator nimA of Aspergillus nidulans. (PubMed id 8274451)1, 2, 3 Schultz S.J. and Nigg E.A. (Cell Growth Differ. 1993)
    2. Protein phosphatase-1alpha regulates centrosome splitting through Nek2. (PubMed id 17283141)1, 2, 9 Mi J.... Larner J.M. (Cancer Res. 2007)
    3. Cell cycle-dependent expression of Nek2, a novel human protein kinase related to the NIMA mitotic regulator of Aspergillus nidulans. (PubMed id 7522034)1, 2, 9 Schultz S.J.... Nigg E.A. (Cell Growth Differ. 1994)
    4. beta-Catenin is a Nek2 substrate involved in centrosome separation. (PubMed id 18086858)1, 2, 9 Bahmanyar S....Barth A.I. (Genes Dev. 2008)
    5. Structure and regulation of the human Nek2 centrosomal kinase. (PubMed id 17197699)1, 2, 9 Rellos P.... Smerdon S.J. (J. Biol. Chem. 2007)
    6. Alternative splice variants of the human centrosome kinase Nek2 exhibit distinct patterns of expression in mitosis. (PubMed id 11742531)1, 2, 9 Hames R.S. and Fry A.M. (Biochem. J. 2002)
    7. Whole genome sequencing in patients with retinitis pigmentosa reveals pathogenic DNA structural changes and NEK2 as a new disease gene. (PubMed id 24043777)1, 3 Nishiguchi K.M....Rivolta C. (Proc. Natl. Acad. Sci. U.S.A. 2013)
    8. Centrosome-related genes, genetic variation, and risk of breast cancer. (PubMed id 20508983)1, 4 Olson J.E....Couch F.J. (Breast Cancer Res. Treat. 2011)
    9. An undecided coiled-coil: the leucine zipper of NEK2 kinase exhibits atypical conformational exchange dynamics. (PubMed id 21669869)1, 2 Croasdale R.... Pfuhl M. (J. Biol. Chem. 2011)
    10. Involvement of a centrosomal protein kendrin in the maintenance of centrosome cohesion by modulating Nek2A kinase activity. (PubMed id 20599736)1, 2 Matsuo K.... Takahashi M. (Biochem. Biophys. Res. Commun. 2010)

    (in PubMed, OMIM, and NCBI Bookshelf)
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     ANDOR
    Aliases
    Disorders
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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    Entrez Gene: 4751 HGNC: 7745 AceView: NEK2 Ensembl:ENSG00000117650 euGenes: HUgn4751
    ECgene: NEK2 H-InvDB: NEK2

    (According to HUGE)
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      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
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    NameDescription
    PharmGKB entry for NEK2 Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
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    Patent Information for NEK2 gene:
    Search GeneIP for patents involving NEK2

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



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