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Aliases for NEK2 Gene

Aliases for NEK2 Gene

  • NIMA-Related Kinase 2 2 3
  • NIMA (Never In Mitosis Gene A)-Related Kinase 2 2 3
  • NimA-Related Protein Kinase 2 3 4
  • NimA-Like Protein Kinase 1 3 4
  • EC 2.7.11.1 4 63
  • NEK2A 3 4
  • RP67 3 6
  • NLK1 3 4
  • Protein Phosphatase 1, Regulatory Subunit 111 3
  • Serine/Threonine-Protein Kinase Nek2 3
  • Never In Mitosis A-Related Kinase 2 4
  • Regulatory Subunit 111 2
  • Protein Phosphatase 1 2
  • EC 2.7.11 63
  • PPP1R111 3
  • HsPK 21 2
  • HSPK 21 4
  • HsPK21 3

External Ids for NEK2 Gene

Previous GeneCards Identifiers for NEK2 Gene

  • GC01M210565
  • GC01M207702
  • GC01M208475
  • GC01M208915
  • GC01M208224
  • GC01M209902
  • GC01M211836
  • GC01M182513

Summaries for NEK2 Gene

Entrez Gene Summary for NEK2 Gene

  • This gene encodes a serine/threonine-protein kinase that is involved in mitotic regulation. This protein is localized to the centrosome, and undetectable during G1 phase, but accumulates progressively throughout the S phase, reaching maximal levels in late G2 phase. Alternatively spliced transcript variants encoding different isoforms with distinct C-termini have been noted for this gene. [provided by RefSeq, Feb 2011]

GeneCards Summary for NEK2 Gene

NEK2 (NIMA-Related Kinase 2) is a Protein Coding gene. Diseases associated with NEK2 include retinitis pigmentosa 67 and van der woude syndrome. Among its related pathways are Cell Cycle, Mitotic and Cell Cycle, Mitotic. GO annotations related to this gene include protein serine/threonine kinase activity and protein phosphatase binding. An important paralog of this gene is NEK1.

UniProtKB/Swiss-Prot for NEK2 Gene

  • Protein kinase which is involved in the control of centrosome separation and bipolar spindle formation in mitotic cells and chromatin condensation in meiotic cells. Regulates centrosome separation (essential for the formation of bipolar spindles and high-fidelity chromosome separation) by phosphorylating centrosomal proteins such as CROCC, CEP250 and NINL, resulting in their displacement from the centrosomes. Regulates kinetochore microtubule attachment stability in mitosis via phosphorylation of NDC80. Involved in regulation of mitotic checkpoint protein complex via phosphorylation of CDC20 and MAD2L1. Plays an active role in chromatin condensation during the first meiotic division through phosphorylation of HMGA2. Phosphorylates: PPP1CC; SGOL1; NECAB3 and NPM1. Essential for localization of MAD2L1 to kinetochore and MAPK1 and NPM1 to the centrosome. Isoform 1 phosphorylates and activates NEK11 in G1/S-arrested cells. Isoform 2, which is not present in the nucleolus, does not.

Gene Wiki entry for NEK2 Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for NEK2 Gene

Genomics for NEK2 Gene

Regulatory Elements for NEK2 Gene

Genomic Location for NEK2 Gene

Start:
211,658,256 bp from pter
End:
211,675,630 bp from pter
Size:
17,375 bases
Orientation:
Minus strand

Genomic View for NEK2 Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for NEK2 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for NEK2 Gene

Proteins for NEK2 Gene

  • Protein details for NEK2 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P51955-NEK2_HUMAN
    Recommended name:
    Serine/threonine-protein kinase Nek2
    Protein Accession:
    P51955
    Secondary Accessions:
    • Q53FD6
    • Q5I1Z9
    • Q5VXZ1
    • Q6NZX8
    • Q7Z634
    • Q86XH2
    • Q96QN9

    Protein attributes for NEK2 Gene

    Size:
    445 amino acids
    Molecular mass:
    51763 Da
    Cofactor:
    Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
    Quaternary structure:
    • Isoform 1, isoform 2 and isoform 4 form homo- and heterodimers. Interacts with NECAB3 and HMGA2 (By similarity). Isoform 1 interacts with CDC20, CTNB1, MAD1L1, MAPK, NEK11, NPM1, NDC80, PCNT and SGOL1. Isoform 1 interacts with STK3/MST2 (via SARAH domain) and SAV1 (via SARAH domain). Isoform 1 and isoform 2 interact with MAD2L1. Isoform 1 and isoform 4 interact with PPP1CA and PPP1CC.
    SequenceCaution:
    • Sequence=AAH65932.1; Type=Frameshift; Positions=213; Evidence={ECO:0000305};

    Three dimensional structures from OCA and Proteopedia for NEK2 Gene

    Alternative splice isoforms for NEK2 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for NEK2 Gene

Proteomics data for NEK2 Gene at MOPED

Selected DME Specific Peptides for NEK2 Gene

Post-translational modifications for NEK2 Gene

  • Activated by autophosphorylation. Protein phosphatase 1 represses autophosphorylation and activation of isoform 1 by dephosphorylation. Phosphorylation by STK3/MST2 is necessary for its localization to the centrosome.
  • Ubiquitination at Lys37, Lys152, Lys174, Lys359, and Lys362
  • Modification sites at PhosphoSitePlus

Other Protein References for NEK2 Gene

ENSEMBL proteins:
Reactome Protein details:
REFSEQ proteins:

Domains for NEK2 Gene

Protein Domains for NEK2 Gene

Suggested Antigen Peptide Sequences for NEK2 Gene

Graphical View of Domain Structure for InterPro Entry

P51955

UniProtKB/Swiss-Prot:

NEK2_HUMAN :
  • P51955
Domain:
  • The leucine-zipper domain is required for its dimerization and activation.
  • Contains 1 protein kinase domain.
Family:
  • Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
genes like me logo Genes that share domains with NEK2: view

Function for NEK2 Gene

Molecular function for NEK2 Gene

GENATLAS Biochemistry: never in mitosis gene A-related kinase 2,Aspergillus nidulans NIMA ortholog,located to the centrosome,expressed in a cell-cycle dependent pattern,undetectable during G1,appearing in S,reacting maximum levels in late G2
UniProtKB/Swiss-Prot CatalyticActivity: ATP + a protein = ADP + a phosphoprotein
UniProtKB/Swiss-Prot EnzymeRegulation: Isoform 1 is inhibited by ionizing radiation in the presence of PPP1CA. Its catalytic activity is inhibited by the inhibitor CCT241950. In the presence of this inhibitor, displays an autoinhibited conformation: Tyr-70 side chain points into the active site, interacts with the activation loop, and blocks the alphaC helix.
UniProtKB/Swiss-Prot Function: Protein kinase which is involved in the control of centrosome separation and bipolar spindle formation in mitotic cells and chromatin condensation in meiotic cells. Regulates centrosome separation (essential for the formation of bipolar spindles and high-fidelity chromosome separation) by phosphorylating centrosomal proteins such as CROCC, CEP250 and NINL, resulting in their displacement from the centrosomes. Regulates kinetochore microtubule attachment stability in mitosis via phosphorylation of NDC80. Involved in regulation of mitotic checkpoint protein complex via phosphorylation of CDC20 and MAD2L1. Plays an active role in chromatin condensation during the first meiotic division through phosphorylation of HMGA2. Phosphorylates: PPP1CC; SGOL1; NECAB3 and NPM1. Essential for localization of MAD2L1 to kinetochore and MAPK1 and NPM1 to the centrosome. Isoform 1 phosphorylates and activates NEK11 in G1/S-arrested cells. Isoform 2, which is not present in the nucleolus, does not.
UniProtKB/Swiss-Prot Induction: Expression and activity peak in the G2 phase of the mitotic cycle and decrease once the cells have entered mitosis due to degradation by the anaphase promoting complex APC/C-CDC20. In G1 phase, both isoform 1 and isoform 2 are almost undetectable. However, at the G1/S transition, there is an increase in expression of both isoforms which then remain at this increased level throughout S and G2. At the onset of mitosis, isoform 1 undergoes a rapid disappearance whereas isoform 2 continues to be present at about the same level as in G2. During the rest of mitosis, isoform 1 remains absent, while isoform 2 only begins to decline upon re-entry into the next G1 phase

Enzyme Numbers (IUBMB) for NEK2 Gene

Gene Ontology (GO) - Molecular Function for NEK2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004672 protein kinase activity IMP 24554434
GO:0004674 protein serine/threonine kinase activity TAS 16280549
GO:0004713 protein tyrosine kinase activity --
GO:0005515 protein binding IPI 10880350
GO:0005524 ATP binding IEA --
genes like me logo Genes that share ontologies with NEK2: view

Phenotypes for NEK2 Gene

genes like me logo Genes that share phenotypes with NEK2: view

miRNA for NEK2 Gene

miRTarBase miRNAs that target NEK2

No data available for Animal Models , Transcription Factor Targeting and HOMER Transcription for NEK2 Gene

Localization for NEK2 Gene

Subcellular locations from UniProtKB/Swiss-Prot for NEK2 Gene

Isoform 1: Nucleus. Nucleus, nucleolus. Cytoplasm. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton, spindle pole. Chromosome, centromere, kinetochore. Chromosome, centromere. Note=STK3/MST2 and SAV1 are required for its targeting to the centrosome. Colocalizes with SGOL1 and MAD1L1 at the kinetochore. Not associated with kinetochore in the interphase but becomes associated with it upon the breakdown of the nuclear envelope. Has a nucleolar targeting/ retention activity via a coiled-coil domain at the C-terminal end.
Isoform 2: Cytoplasm. Note=Predominantly cytoplasmic.
Isoform 4: Nucleus. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Note=Predominantly nuclear.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for NEK2 Gene COMPARTMENTS Subcellular localization image for NEK2 gene
Compartment Confidence
cytoskeleton 5
nucleus 5
cytosol 4
peroxisome 1

Gene Ontology (GO) - Cellular Components for NEK2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000776 colocalizes_with kinetochore IDA 17621308
GO:0000777 condensed chromosome kinetochore IEA --
GO:0000794 condensed nuclear chromosome IEA --
GO:0000922 spindle pole IEA --
GO:0005634 nucleus IDA --
genes like me logo Genes that share ontologies with NEK2: view

Pathways for NEK2 Gene

genes like me logo Genes that share pathways with NEK2: view

Gene Ontology (GO) - Biological Process for NEK2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000070 mitotic sister chromatid segregation IEA --
GO:0000086 G2/M transition of mitotic cell cycle TAS --
GO:0000278 mitotic cell cycle TAS --
GO:0001824 blastocyst development IEA --
GO:0006468 protein phosphorylation IMP 24554434
genes like me logo Genes that share ontologies with NEK2: view

Compounds for NEK2 Gene

(3) HMDB Compounds for NEK2 Gene

Compound Synonyms Cas Number PubMed IDs
Adenosine triphosphate
  • 5'-(tetrahydrogen triphosphate) Adenosine
56-65-5
ADP
  • adenosindiphosphorsaeure
58-64-0
Magnesium
  • Magnesium
7439-95-4

(1) Drugbank Compounds for NEK2 Gene

(2) Novoseek inferred chemical compound relationships for NEK2 Gene

Compound -log(P) Hits PubMed IDs
threonine 52.6 6
serine 46 9
genes like me logo Genes that share compounds with NEK2: view

Transcripts for NEK2 Gene

Unigene Clusters for NEK2 Gene

NIMA-related kinase 2:
Representative Sequences:

Alternative Splicing Database (ASD) splice patterns (SP) for NEK2 Gene

ExUns: 1 ^ 2 ^ 3a · 3b ^ 4 ^ 5a · 5b · 5c ^ 6a · 6b ^ 7a · 7b · 7c · 7d · 7e ^ 8a · 8b ^ 9a · 9b · 9c · 9d · 9e
SP1: - - -
SP2: - - - -
SP3: - - - -
SP4: - - - - - - -
SP5: - - - -
SP6: -
SP7:

Relevant External Links for NEK2 Gene

GeneLoc Exon Structure for
NEK2
ECgene alternative splicing isoforms for
NEK2

Expression for NEK2 Gene

mRNA expression in normal human tissues for NEK2 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for NEK2 Gene

This gene is overexpressed in Testis (17.5).

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, MOPED, and MaxQB for NEK2 Gene

SOURCE GeneReport for Unigene cluster for NEK2 Gene Hs.153704

mRNA Expression by UniProt/SwissProt for NEK2 Gene

P51955-NEK2_HUMAN
Tissue specificity: Isoform 1 and isoform 2 are expressed in peripheral blood T-cells and a wide variety of transformed cell types. Isoform 1 and isoform 4 are expressed in the testis. Up-regulated in various cancer cell lines, as well as primary breast tumors.
genes like me logo Genes that share expressions with NEK2: view

Orthologs for NEK2 Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for NEK2 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia NEK2 35
  • 99.78 (n)
  • 99.78 (a)
NEK2 36
  • 100 (a)
OneToOne
cow
(Bos Taurus)
Mammalia NEK2 35
  • 89.01 (n)
  • 93.24 (a)
NEK2 36
  • 91 (a)
OneToOne
dog
(Canis familiaris)
Mammalia NEK2 35
  • 90.54 (n)
  • 94.37 (a)
NEK2 36
  • 94 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Nek2 35
  • 83.3 (n)
  • 88.49 (a)
Nek2 16
Nek2 36
  • 88 (a)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia NEK2 36
  • 87 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia NEK2 36
  • 82 (a)
OneToOne
chicken
(Gallus gallus)
Aves NEK2 35
  • 71.63 (n)
  • 77.43 (a)
NEK2 36
  • 77 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia NEK2 36
  • 78 (a)
OneToOne
African clawed frog
(Xenopus laevis)
Amphibia MGC53202 35
tropical clawed frog
(Silurana tropicalis)
Amphibia nek2 35
  • 68.11 (n)
  • 73.35 (a)
Str.3912 35
rainbow trout
(Oncorhynchus mykiss)
Actinopterygii Omy.2217 35
zebrafish
(Danio rerio)
Actinopterygii nek2 35
  • 64.84 (n)
  • 69.11 (a)
nek2 36
  • 68 (a)
OneToOne
zgc55602 35
fruit fly
(Drosophila melanogaster)
Insecta Nek2 36
  • 24 (a)
OneToOne
Nek2 37
  • 48 (a)
worm
(Caenorhabditis elegans)
Secernentea ZC581.1 37
  • 36 (a)
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes KIN3 36
  • 34 (a)
OneToMany
KIN3 38
fission yeast
(Schizosaccharomyces pombe)
Schizosaccharomycetes fin1 35
  • 49.19 (n)
  • 42.12 (a)
sea squirt
(Ciona intestinalis)
Ascidiacea Cin.11998 35
sea squirt
(Ciona savignyi)
Ascidiacea -- 36
  • 54 (a)
OneToOne
Species with no ortholog for NEK2:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rat (Rattus norvegicus)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for NEK2 Gene

ENSEMBL:
Gene Tree for NEK2 (if available)
TreeFam:
Gene Tree for NEK2 (if available)

Paralogs for NEK2 Gene

Paralogs for NEK2 Gene

Selected SIMAP similar genes for NEK2 Gene using alignment to 2 proteins:

Pseudogenes.org Pseudogenes for NEK2 Gene

genes like me logo Genes that share paralogs with NEK2: view

Variants for NEK2 Gene

Sequence variations from dbSNP and Humsavar for NEK2 Gene

SNP ID Clin Chr 01 pos Sequence Context AA Info Type MAF
rs15305 -- 211,663,283(-) TGGTT(A/T)TACTG intron-variant, utr-variant-3-prime
rs697003 -- 211,669,734(-) ACAGA(C/G)AAGGA intron-variant
rs697004 -- 211,669,466(-) CAAGT(G/T)TTTAA intron-variant
rs701927 -- 211,675,980(-) AGGAA(G/T)GCGTG upstream-variant-2KB
rs701928 -- 211,675,884(-) GGAGG(A/T)CCTTA upstream-variant-2KB

Relevant External Links for NEK2 Gene

HapMap Linkage Disequilibrium report
NEK2
Human Gene Mutation Database (HGMD)
NEK2

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot and Structural Variations from Database of Genomic Variants (DGV) for NEK2 Gene

Disorders for NEK2 Gene

(1) OMIM Diseases for NEK2 Gene (604043)

UniProtKB/Swiss-Prot

NEK2_HUMAN
  • Retinitis pigmentosa 67 (RP67) [MIM:615565]: A retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. {ECO:0000269 PubMed:24043777}. Note=The disease is caused by mutations affecting the gene represented in this entry.

(3) Novoseek inferred disease relationships for NEK2 Gene

Disease -log(P) Hits PubMed IDs
cancer 16.4 5
breast cancer 0 1
tumors 0 2

Relevant External Links for NEK2

Genetic Association Database (GAD)
NEK2
Human Genome Epidemiology (HuGE) Navigator
NEK2
genes like me logo Genes that share disorders with NEK2: view

Publications for NEK2 Gene

  1. Cell cycle-dependent expression of Nek2, a novel human protein kinase related to the NIMA mitotic regulator of Aspergillus nidulans. (PMID: 7522034) Schultz S.J. … Nigg E.A. (Cell Growth Differ. 1994) 3 4 23
  2. Alternative splice variants of the human centrosome kinase Nek2 exhibit distinct patterns of expression in mitosis. (PMID: 11742531) Hames R.S. … Fry A.M. (Biochem. J. 2002) 3 4 23
  3. Structure and regulation of the human Nek2 centrosomal kinase. (PMID: 17197699) Rellos P. … Smerdon S.J. (J. Biol. Chem. 2007) 3 4 23
  4. Protein phosphatase-1alpha regulates centrosome splitting through Nek2. (PMID: 17283141) Mi J. … Larner J.M. (Cancer Res. 2007) 3 4 23
  5. beta-Catenin is a Nek2 substrate involved in centrosome separation. (PMID: 18086858) Bahmanyar S. … Barth A.I. (Genes Dev. 2008) 3 4 23

Products for NEK2 Gene

  • Addgene plasmids for NEK2

Sources for NEK2 Gene

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