NECAP2 Gene
protein-coding GIFtS: 50
GCID: GC01P016767
|
|
NECAP endocytosis associated 2
| |
Aliases
for NECAP2 gene
(According to
1HGNC,
2Entrez Gene,
3UniProtKB/Swiss-Prot,
4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc,
7Ensembl,
8DME,
9miRBase,
and/or 10fRNAdb)
About This Section
|
| Aliases |
|---|
| NECAP Endocytosis Associated 21 2 | | NECAP-22 3 | | NECAP Endocytosis-Associated Protein 22 3 | | FLJ104201 | | Adaptin Ear-Binding Coat-Associated Protein 22 | | Adaptin-Ear-Binding Coat-Associated Protein 22 |
Export aliases for NECAP2 gene to outside databasesPrevious GC identifers: GC01P016513 GC01P016639 GC01P015284 |
Summaries
for NECAP2 gene(According to Entrez Gene,
Tocris Bioscience,
Wikipedia's
Gene Wiki,
PharmGKB,
UniProtKB/Swiss-Prot,
and/or
UniProtKB/TrEMBL)
About This Section
|
Entrez Gene summary for NECAP2:
This gene likely encodes a member of the adaptin-ear-binding coat-associated protein family. Studies of a similarprotein in rat suggest a role in clathrin-mediated endocytosis. Alternatively spliced transcript variants have beendescribed. (provided by RefSeq, Feb 2009)
UniProtKB/Swiss-Prot: NECP2_HUMAN, Q9NVZ3Function: Involved in endocytosis (By similarity)
Gene Wiki entry for NECAP2
|
Genomic Views
for NECAP2 gene
(According to
GeneLoc and/or
HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to
UCSC (hg19) and
Ensembl (release 69),
Regulatory elements and Epigenetics data according to
QIAGEN,
SABiosciences, and/or
SwitchGear Genomics)
About This Section
| RefSeq DNA sequence:- NC_000001.10 NC_018912.1 NT_004610.19
Regulatory elements:
 SABiosciences Regulatory transcription factor binding sites in the NECAP2 gene promoter:
SRF AML1a HTF SRF (504 AA) STAT1alpha RORalpha2 POU2F1 POU2F1a LyF-1 Pax-4a
Other transcription factors
Search SABiosciences Chromatin IP Primers for NECAP2
Epigenetics:
| | QIAGEN PyroMark CpG Assay predesigned Pyrosequencing DNA Methylation assays in human, mouse, rat NECAP2 |
Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track
Entrez Gene cytogenetic band: 1p36.13 Ensembl cytogenetic band: 1p36.13 HGNC cytogenetic band: 1p36.13NECAP2 Gene in genomic location: bands according to Ensembl, locations according to
 (and/or Entrez Gene and/or Ensembl if different)
GeneLoc information about chromosome 1 GeneLoc Exon Structure GeneLoc location for GC01P016767: view genomic region
(about GC identifiers)
Start:
|
16,767,167 bp from pter |
End:
|
16,786,585 bp from pter |
Size:
|
19,419 bases |
Orientation:
|
plus strand |
|
Proteins
for NECAP2 gene
(According to
1UniProtKB,
HORDE,
neXtProt,
Ensembl,
and/or Reactome,
Modification sites according to 2PhosphoSitePlus,
Specific Peptides from DME,
Protein expression images according to data from SPIRE MOPED and PaxDb,
RefSeq according to NCBI,
PDB rendering according to OCA and/or Proteopedia,
Recombinant Proteins
from
EMD Millipore,
R&D Systems,
GenScript,
Enzo Life Sciences,
OriGene,
Novus Biologicals,
Sino Biological,
ProSpec, and/or
Uscn,
Biochemical Assays by
EMD Millipore,
R&D Systems,
OriGene,
GenScript,
Cell Signaling Technology,
Enzo Life Sciences, and/or
Uscn,
Ontologies according to Gene
Ontology Consortium 01 Mar 2013 and
Entrez Gene,
Antibodies by
EMD Millipore,
R&D Systems,
GenScript,
Cell Signaling Technology,
OriGene,
Novus Biologicals,
Thermo Fisher Scientific,
Abcam, and/or
Uscn)
About This Section
|
UniProtKB/Swiss-Prot: NECP2_HUMAN, Q9NVZ3 (See
protein sequence)Recommended Name: Adaptin ear-binding coat-associated protein 2 Size: 263 amino acids; 28339 Da
Subunit: Interacts with AP1G1 and AP2A1 components of the adapter protein complexes AP-1 and AP-2. Interacts with theGAE domain proteins GGA1, GGA2 and GGA3 (By similarity)
Subcellular location: Cytoplasmic vesicle, clathrin-coated vesicle membrane (By similarity). Cell membrane (Bysimilarity). Note=Colocalizes with AP-2 at the plasma membrane (By similarity)
Sequence caution: Sequence=BAB15758.1; Type=Erroneous initiation; Note=Translation N-terminally shortened;
Secondary accessions: B4DY19 E9PGQ8 Q5VSU4 Q5VSU5 Q9H7L1 Q9H8L1Alternative splicing: 4 isoforms: Q9NVZ3-1 Q9NVZ3-2 Q9NVZ3-3 Q9NVZ3-4 (No experimental confirmation available)Explore the universe of human proteins at neXtProt for NECAP2: NX_Q9NVZ3
Post-translational modifications:
View modification sites using PhosphoSitePlus2View neXtProt modification sites for NX_Q9NVZ3
NECAP2 Protein expression data from MOPED and PaxDb: About this image 
REFSEQ proteins (3 alternative transcripts):
NP_001138749.1 NP_001138750.1 NP_060560.1
ENSEMBL proteins: ENSP00000338746 ENSP00000424509 ENSP00000407091 ENSP00000391942 ENSP00000421867 ENSP00000427620 ENSP00000421335 ENSP00000423213 ENSP00000383925
Human Recombinant Protein Products:
Gene Ontology (GO): 5 cellular component terms (GO ID links to tree view): About this table
NECAP2 for ontologies About GeneDecksing
NECAP2 Antibody Products:
Assay Products for NECAP2: |
Protein
Domains /
Families
for NECAP2 gene(According to InterPro, ProtoNet,
UniProtKB, and/or BLOCKS,
Sets of similar genes according to GeneDecks)
About This Section
|
NECAP2 for domains About GeneDecksing
2 InterPro domains/families:Graphical View of Domain Structure for InterPro Entry Q9NVZ3ProtoNet protein and cluster: Q9NVZ3 1 Blocks protein family: IPB012466 Protein of unknown function DUF1681
UniProtKB/Swiss-Prot: NECP2_HUMAN, Q9NVZ3Domain: The WXXF motifs mediate binding of accessory proteins to the ear-domain of AP-1, GGAs and AP-2 throughhydrophobic interactions. Selective binding to the GAE domains of AP-1 or to the alpha-ear domain of AP-2 is tuned bythe acidic context surrounding the motif and the properties of the second residue of the motif itself (By similarity)Similarity: Belongs to the NECAP family |
Function
for NECAP2 gene
(According to 1UniProtKB,
Genatlas,
LifeMap Discovery™,
IUBMB, and/or
2DME,
Human phenotypes from GenomeRNAi,
Animal models from MGI Mar 06 2013,
bound targets from SABiosciences,
miRNA Gene Targets from miRTarBase
shRNA from
OriGene,
RNAi from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
microRNA from QIAGEN,
Gene Editing from DNA2.0,
Clones from EMD Millipore,
OriGene,
SwitchGear Genomics,
GenScript,
Sino Biological,
DNA2.0,
and Vector BioLabs,
Cell Lines from GenScript,
LifeMap BioReagents,
In Situ Hybridization Assays from Advanced Cell Diagnostics,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene.)
About This Section
|
Function Summary:
UniProtKB/Swiss-Prot: NECP2_HUMAN, Q9NVZ3Function: Involved in endocytosis (By similarity)
Clone
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OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for NECAP2 (see all 3)
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
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In Situ Assay
Products: |
 | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for NECAP2 |
2 GenomeRNAi human phenotypes for NECAP2:
|
Pathways & Interactions
for NECAP2 gene
(Pathways according to
EMD Millipore,
R&D Systems,
Cell Signaling Technology,
KEGG,
PharmGKB,
BioSystems,
Reactome,
Tocris Bioscience,
GeneGo (Thomson Reuters),
QIAGEN,
and/or UniProtKB,
Sets of similar genes according to GeneDecks,
Interaction Networks according to
SABiosciences,
and/or STRING,
Interactions according to 1UniProtKB,
2MINT,
3I2D, and/or
4STRING,
with links to IntAct and
Ensembl,
Ontologies according to Gene Ontology Consortium 01 Mar 2013 via
Entrez Gene).
About This Section
|
Interactions:
Search SABiosciences Gene Network CentralTM Interacting Genes and Proteins Networks for NECAP2
5/11 Interacting proteins for NECAP2 (Q9NVZ32, 3) via UniProtKB, MINT, STRING, and/or I2D (see all 11)About this table
Gene Ontology (GO): 2 biological process terms (GO ID links to tree view): About this table | GO ID | Qualified GO term | Evidence | PubMed IDs |
|---|
| GO:0006897 | endocytosis |
ISS | -- | | GO:0015031 | protein transport |
IEA | -- |
NECAP2 for ontologies About GeneDecksing
|
Drugs & Compounds
for NECAP2 gene(Chemical Compounds according to UniProtKB, Enzo Life Sciences,
EMD Millipore, Tocris Bioscience
HMDB,
BitterDB, and/or
Novoseek, and Drugs according to
DrugBank,
Enzo Life Sciences, and/or
PharmGKB, with drugs/clinical trials/news
search links to CenterWatch)
About This Section
|
 Browse Tocris compounds for NECAP2
Search CenterWatch for drugs/clinical trials and news about NECAP2 / NECP2
|
Transcripts
for NECAP2 gene(Secondary structures according to
fRNAdb,
GenBank/EMBL/DDBJ Accessions according to
Unigene
(Build 235 Homo sapiens; Mar 10 2013) or GenBank,
RefSeq according to Entrez Gene,
DOTS (version 10), and/or
AceView,
transcript ids from Ensembl
with links to UCSC,
exon structure from GeneLoc,
alternative splicing isoforms according to ASD and/or
ECgene,
RNAi Products from
EMD Millipore,
siRNAs from
OriGene,
QIAGEN,
shRNA from
OriGene,
microRNA from QIAGEN,
Tagged/untagged cDNA clones from
OriGene,
SwitchGear Genomics,
GenScript,
DNA2.0,
Vector BioLabs,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN
)
About This Section
|
REFSEQ mRNAs for NECAP2 gene (3 alternative transcripts): NM_001145277.1 NM_001145278.1 NM_018090.4 Unigene Cluster for NECAP2: NECAP endocytosis associated 2 Hs.437385 [show with all ESTs]Unigene Representative Sequence: NM_01809013 Ensembl transcripts including schematic representations, and UCSC links where relevant: ENST00000337132(uc001ayo.3 uc010ocd.2 uc001ayq.3 uc021ogo.1)
ENST00000504551 ENST00000457722 ENST00000486390 ENST00000443980 ENST00000504858
ENST00000508680 ENST00000492095 ENST00000459640 ENST00000496239 ENST00000513161
ENST00000509727 ENST00000406746
Clone
Products: | | OriGene Myc/DDK tagged cDNA clones in CMV expression vector in human, mouse, rat for NECAP2 (see all 5)
OriGene untagged cDNA clones in CMV expression vector in human, mouse, rat for NECAP2 (see all 3)
OriGene custom cloning services – gene synthesis, subcloning, mutagenesis,
variant library, vector shuttling
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Additional cDNA sequence: AK001282.1 AK021938.1 AK023545.1 AK223344.1 AK300764.1 AK302227.1 BC017014.1 BC018914.2 19 DOTS entries: DT.100866337 DT.91751106 DT.100030072 DT.218377 DT.91713835 DT.100652109 DT.40107593 DT.95157983 DT.100869077 DT.409313 DT.95158002 DT.100640434 DT.100652108 DT.100866753 DT.121346660 DT.40308785 DT.91729007 DT.95157995 DT.95196312 24/446 AceView cDNA sequences (see all 446): BQ953637 CR617164 BM456664 BQ671068 BF681656 AI952960 BP379586 AI608836 BX458346 BP363670 BM555821 W35368 CF131675 CR606897 BP369832 BG385473 BQ438152 AA503103 BM684005 BQ013339 AI203076 BQ962339 BX384185 AI187864
GeneLoc Exon Structure
5/8 Alternative Splicing Database (ASD) splice patterns (SP) for NECAP2 (see all 8) About this scheme
| ExUns: | 1a | · | 1b | · | 1c | · | 1d | ^ | 2 | ^ | 3 | ^ | 4 | ^ | 5 | ^ | 6a | · | 6b | ^ | 7 | ^ | 8 | ^ | 9a | · | 9b | ^ | 10a | · | 10b | ^ | 11 |
|---|
|
| SP1: | | | | | | | | | - | | | | | | | | | | - | | - | | | | - | | | | | | | | | | |
| SP2: | | | | | | | | | - | | | | | | | | | | - | | - | | | | - | | | | | | | | | | |
| SP3: | | | | | | | | | - | | | | | | | | | | - | | | | | | - | | | | | | | | | | |
| SP4: | | | | | | | | | - | | | | | | | | | | - | | - | | | | - | | - | | | | | | | | |
| SP5: | | | | | | | - | | - | | | | | | | | | | - | | - | | | | | | | | | | | | | |
ECgene alternative splicing isoforms for NECAP2
|
Expression
for NECAP2 gene
(RNA expression data according to
H-InvDB,
NONCODE,
miRBase, and
RNAdb,
Expression images according to data from
BioGPS,
Illumina Human BodyMap, and
CGAP
SAGE,
Sets of similar genes according to GeneDecks,
in vivo and in vitro expression data from LifeMap Discovery™,
plus additional links to
Genevestigator, and/or
SOURCE, and/or
BioGPS, and/or
UniProtKB,
PCR Arrays from
SABiosciences,
Primers from
OriGene,
SABiosciences, and/or
QIAGEN,
In Situ Hybridization Assays from Advanced Cell Diagnostics)
About This Section
| NECAP2 expression in normal human tissues (normalized intensities)
See probesets specificity/sensitivity at GeneAnnot
About this imageBioGPS
CGAP TAG: TTCACAGTGC
About this image
See NECAP2 Protein Expression from SPIRE MOPED and PaxDB
Genevestigator expression for NECAP2
SOURCE GeneReport for Unigene cluster: Hs.437385
SABiosciences Custom PCR Arrays for NECAP2
Primer
Products: | | OriGene genome-wide validated SYBR primer pairs in human, mouse, rat for NECAP2
Browse OriGene validated miRNA SYBR primer pairs
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Assay Products: |
| Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for NECAP2 |
Orthologs
for NECAP2 gene
(Orthologs according to
1,2HomoloGene (2older version, for species not in 1newer version),
3euGenes,
4SGD
,
5MGI Mar 06 2013,
with possible further links to
Flybase
and/or
WormBase,
and/or
6Ensembl pan taxonomic compara ,
Gene Trees according to Ensembl and
TreeFam)
About This Section
|
This gene was present in the common ancestor of eukaryotes.
Orthologs for NECAP2 gene from 8/28 species (see all 28) About this table
| Organism |
Taxonomic
classification |
Gene |
Description |
Human
Similarity |
Orthology
Type |
Details |
chicken
(Gallus gallus) |
Aves |
NECAP21 |
NECAP endocytosis associated 2 |
75.52(n)
78.12(a) |
|
419464 NM_001012837.1 NP_001012855.1 |
lizard
(Anolis carolinensis) |
Reptilia |
NECAP26 |
-- |
70(a) |
1 ↔ 1 |
GL345048.1(321-3618) |
African clawed frog
(Xenopus laevis) |
Amphibia |
Xl.41592 |
Xenopus laevis transcribed sequence with weak similarity to protein refNP_060560.1 (H.sapiens) hypothetical protein FLJ10420 [Homo sapiens] less |
76.26(n) |
|
CF520255.1 |
zebrafish
(Danio rerio) |
Actinopterygii |
necap21 |
NECAP endocytosis associated 2 |
64.52(n)
63.71(a) |
|
553536 XM_003200913.1 XP_003200961.1 |
fruit fly
(Drosophila melanogaster) |
Insecta |
CG91326 |
-- |
49(a) |
1 → many |
X(16690931-16694741) |
worm
(Caenorhabditis elegans) |
Secernentea |
CELE_Y110A2AR.31 |
Protein Y110A2AR.3 |
51.98(n)
51.1(a) |
|
173638 NM_061997.4 NP_494398.1 |
thale cress
(Arabidopsis thaliana) |
eudicotyledons |
NAP41 |
non-intrinsic ABC protein 4 |
49.67(n)
42.97(a) |
|
839378 NM_100270.3 NP_563693.1 |
rice
(Oryza sativa) |
Liliopsida |
Os10g04760001 |
hypothetical protein |
50.14(n)
46.75(a) |
|
4348887 NM_001071379.1 NP_001064844.1 |
ENSEMBL Gene Tree for NECAP2 (if available)
TreeFam Gene Tree for NECAP2 (if available) |
Paralogs
for NECAP2 gene(Paralogs according to
1HomoloGene,
2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
About This Section
| Paralogs for NECAP2 gene
- NECAP12
1 SIMAP similar gene for NECAP2 using alignment to 5 protein entries: NECP2_HUMAN (see all proteins):NECAP1
NECAP2 for paralogs About GeneDecksing
|
Genomic Variants
for NECAP2 gene(SNPs/Variants according to the
1NCBI SNP Database,
2Ensembl,
3PupaSUITE,
UniProtKB, and
DNA2.0,
Linkage Disequilibrium by HapMap,
Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene
Mutation Database (HGMD) and the Locus Specific Mutation
Databases (LSDB), Blood group antigen gene mutations by BGMUT,
Resequencing Primers from QIAGEN,
Cancer Mutation PCR Arrays and Assays and Copy Number PCR Arrays from SABiosciences)
About This Section
|
| Genomic Data | Transcription Related Data | Allele Frequencies | | SNP ID | Valid | Clinical
significance | Chr 1 pos | Sequence | # | AA
Chg | Type | More | # | Allele
freq | Pop | Total
sample | More |
|---|
HapMap Linkage Disequilibrium report for NECAP2 (16767167 - 16786585 bp)
Structural Variations (Copy Number Variations, Insertions/Deletions, Inversions)
Database of Genomic Variants (DGV): 3 variations for NECAP2
2 CNVs: 3284 34489
1 Indel: 33500 | SABiosciences Cancer Mutation PCR Assays |
| | QIAGEN SeqTarget long-range PCR primers in human, mouse, rat for resequencing NECAP2 |
|
Disorders
/ Diseases
for NECAP2 gene
(in which this Gene is Involved, According to MalaCards,
OMIM, UniProtKB,
the University of Copenhagen DISEASES
database,
Genatlas, GeneTests,
GAD,
HuGE Navigator,
and/or TGDB.)
About This Section
|
 NECAP2 for disorders About MalaCards
NECAP2 for disorders About GeneDecksing
OMIM gene information: 611624
OMIM disorders: --
Export disorders for NECAP2 gene to outside databases
|
Publications
for NECAP2 gene (in
PubMed.
Associations of this gene to articles via
1Entrez Gene,
2UniProtKB/Swiss-Prot,
3HGNC,
4GAD,
5PharmGKB,
6HMDB,
7DrugBank,
8UniProtKB/TrEMBL,
9 Novoseek, and/or
10fRNAdb)
About This Section
|
PubMed articles for NECAP2 gene, integrated from 9 sources (see all 22):
(articles sorted by number of sources associating them with NECAP2) | |  | Utopia: connect your pdf to the dynamic
world of online information |
- The DNA sequence and biological annotation of human chromosome 1. (PubMed id 16710414)1, 2 Gregory S.G.... Bentley D.R. (2006)
- The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PubMed id 15489334)1, 2 Gerhard D.S....Malek J. (2004)
- Complete sequencing and characterization of 21,243 full-length human cDNAs. (PubMed id 14702039)1, 2 Ota T.... Sugano S. (2004)
- Identification of a family of endocytic proteins that define a new alpha-adaptin ear-binding motif. (PubMed id 14555962)1, 3 Ritter B....McPherson P.S. (2003)
- Characterization of long cDNA clones from human adult spleen. (PubMed id 11214971)1, 2 Hattori A.... Ohara O. (2000)
- A census of human soluble protein complexes. (PubMed id 22939629)1 Havugimana P.C....Emili A. (2012)
- Initial characterization of the human central proteome. (PubMed id 21269460)2 Burkard T.R.... Colinge J. (2011)
- Interactions of pathological hallmark proteins: tubul in polymerization promoting protein/p25, beta-amyloid, and alpha-synuclein. (PubMed id 21832049)1 OlA!h J....OvA!di J. (2011)
- Mass spectrometric analysis of lysine ubiquitylation r eveals promiscuity at site level. (PubMed id 21139048)1 Danielsen J.M....Nielsen M.L. (2011)
- Charting the molecular network of the drug target Bcr-Abl. (PubMed id 19380743)1 Brehme M....Superti-Furga G. (2009)
|
External Searches for NECAP2 gene
(in PubMed,
OMIM, and NCBI Bookshelf)
About This Section
|
|
Genome Databases showing NECAP2 gene
(According to
Entrez Gene,
HGNC,
AceView,
euGenes,
Ensembl,
miRBase,
ECgene,
Kegg,
and/or
H-InvDB)
About This Section
|
|
Other Databases showing NECAP2 gene
(According to HUGE)
About This Section
| -- |
Specialized Databases showing NECAP2 gene(According to PharmGKB,
ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL,
Wikipedia and/or GeneReviews via UniProtKB/Swiss-Prot)
About This Section
|
|
About This Section
| Patent Information for NECAP2 gene:
Search GeneIP for patents involving NECAP2
GeneCards and IP:
Japan Patent Office Licenses GeneCards European Patent Office Licenses GeneCards Improving the IP Search
|
Products
for NECAP2 gene(Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, SABiosciences, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Uscn, Thermo Fisher Scientific, Gene Editing from DNA2.0, Clones from EMD Millipore, OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript and LifeMap BioReagents, PCR Arrays from SABiosciences, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, and/or
Enzo Life Sciences),
In Situ Hybridization Assays from
Advanced Cell Diagnostics
About This Section
|
 | |
 | |
 |
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| |
 |
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| |
 |  |  |  | |
| | | | Search Tocris compounds for NECAP2 |
| |  |  |  |  | | | | | | Recombinant Protein for NECAP2 |
|
 |
 |
 |
 | | | NECAP2 Proteins, Antibodies, CLIAs, and ELISAs |
| | | | | Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for NECAP2 |
|  |  |  | | | | Search ThermoFisher Antibodies for NECAP2 |
| | | Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat NECAP2 |
|
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