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Aliases for NDST2 Gene

Aliases for NDST2 Gene

  • N-Deacetylase/N-Sulfotransferase (Heparan Glucosaminyl) 2 2 3
  • Glucosaminyl N-Deacetylase/N-Sulfotransferase 2 3 4
  • N-Heparan Sulfate Sulfotransferase 2 3 4
  • N-HSST 2 3 4
  • NDST-2 3 4
  • HSST2 3 4
  • Bifunctional Heparan Sulfate N-Deacetylase/N-Sulfotransferase 2 3
  • EC 2.8.2.8 4
  • NST2 3

External Ids for NDST2 Gene

Previous GeneCards Identifiers for NDST2 Gene

  • GC10M074340
  • GC10M074665
  • GC10M075454
  • GC10M074906
  • GC10M075231
  • GC10M075561
  • GC10M069557

Summaries for NDST2 Gene

Entrez Gene Summary for NDST2 Gene

  • This gene encodes a member of the N-deacetylase/N-sulfotransferase subfamily of the sulfotransferase 1 proteins. The encoded enzyme has dual functions in processing glucosamine and heparin polymers, including N-deacetylation and N-sulfation. The encoded protein may be localized to the Golgi. [provided by RefSeq, Feb 2009]

GeneCards Summary for NDST2 Gene

NDST2 (N-Deacetylase/N-Sulfotransferase (Heparan Glucosaminyl) 2) is a Protein Coding gene. Among its related pathways are Disease and Metabolism. GO annotations related to this gene include deacetylase activity and [heparan sulfate]-glucosamine N-sulfotransferase activity. An important paralog of this gene is NDST4.

UniProtKB/Swiss-Prot for NDST2 Gene

  • Essential bifunctional enzyme that catalyzes both the N-deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA disaccharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis. Plays a role in determining the extent and pattern of sulfation of heparan sulfate.

Gene Wiki entry for NDST2 Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for NDST2 Gene

Genomics for NDST2 Gene

Regulatory Elements for NDST2 Gene

Genomic Location for NDST2 Gene

Start:
73,801,911 bp from pter
End:
73,811,831 bp from pter
Size:
9,921 bases
Orientation:
Minus strand

Genomic View for NDST2 Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for NDST2 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for NDST2 Gene

Proteins for NDST2 Gene

  • Protein details for NDST2 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P52849-NDST2_HUMAN
    Recommended name:
    Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 2
    Protein Accession:
    P52849
    Secondary Accessions:
    • Q2TB32
    • Q59H89

    Protein attributes for NDST2 Gene

    Size:
    883 amino acids
    Molecular mass:
    100875 Da
    Quaternary structure:
    • Monomer.
    Miscellaneous:
    • The presence of 4 different heparan sulfate N-deacetylase/N-sulfotransferase enzymes in mammals, as well as differences in their enzyme activity suggest that some initiate heparan sulfate modification/sulfation reactions, whereas other later on fill in or extend already modified heparan sulfate sequences

    Alternative splice isoforms for NDST2 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for NDST2 Gene

Proteomics data for NDST2 Gene at MOPED

Post-translational modifications for NDST2 Gene

  • Glycosylation at Asn233, Asn350, Asn400, Asn666, Asn726, and Asn802
  • Modification sites at PhosphoSitePlus

Other Protein References for NDST2 Gene

ENSEMBL proteins:
Reactome Protein details:
REFSEQ proteins:

No data available for DME Specific Peptides for NDST2 Gene

Domains for NDST2 Gene

Gene Families for NDST2 Gene

HGNC:
  • SULTM :Sulfotransferases, membrane-bound

Protein Domains for NDST2 Gene

Graphical View of Domain Structure for InterPro Entry

P52849

UniProtKB/Swiss-Prot:

NDST2_HUMAN :
  • P52849
Family:
  • Belongs to the sulfotransferase 1 family. NDST subfamily.
genes like me logo Genes that share domains with NDST2: view

Function for NDST2 Gene

Molecular function for NDST2 Gene

GENATLAS Biochemistry: heparan sulfate-N-deacetylase/N-sulfotransferase 2,with two alternative forms,predominantly expressed in heart,liver,pancreas,located to the trans-Golgi network
UniProtKB/Swiss-Prot BiophysicochemicalProperties: Kinetic parameters: KM=4.7 uM for K5 polysaccharide {ECO:0000269 PubMed:12634318}; KM=0.76 uM for N-acetylated HS-II {ECO:0000269 PubMed:12634318};
UniProtKB/Swiss-Prot CatalyticActivity: 3-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine = adenosine 3,5-bisphosphate + [heparan sulfate]-N-sulfoglucosamine
UniProtKB/Swiss-Prot Function: Essential bifunctional enzyme that catalyzes both the N-deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA disaccharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis. Plays a role in determining the extent and pattern of sulfation of heparan sulfate.

Enzyme Numbers (IUBMB) for NDST2 Gene

Gene Ontology (GO) - Molecular Function for NDST2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0008146 sulfotransferase activity --
GO:0015016 [heparan sulfate]-glucosamine N-sulfotransferase activity IEA --
GO:0016787 hydrolase activity IEA --
genes like me logo Genes that share ontologies with NDST2: view
genes like me logo Genes that share phenotypes with NDST2: view

Animal Models for NDST2 Gene

MGI Knock Outs for NDST2:

Animal Model Products

CRISPR Products

No data available for miRNA , Transcription Factor Targeting and HOMER Transcription for NDST2 Gene

Localization for NDST2 Gene

Subcellular locations from UniProtKB/Swiss-Prot for NDST2 Gene

Golgi apparatus membrane; Single-pass type II membrane protein.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for NDST2 Gene COMPARTMENTS Subcellular localization image for NDST2 gene
Compartment Confidence
golgi apparatus 5
extracellular 2
cytosol 1
endoplasmic reticulum 1
lysosome 1

Gene Ontology (GO) - Cellular Components for NDST2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000139 Golgi membrane TAS --
GO:0016021 integral component of membrane IEA --
genes like me logo Genes that share ontologies with NDST2: view

Pathways for NDST2 Gene

genes like me logo Genes that share pathways with NDST2: view

UniProtKB/Swiss-Prot P52849-NDST2_HUMAN

  • Pathway: Glycan metabolism; heparan sulfate biosynthesis
  • Pathway: Glycan metabolism; heparin biosynthesis

Gene Ontology (GO) - Biological Process for NDST2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005975 carbohydrate metabolic process TAS --
GO:0006024 glycosaminoglycan biosynthetic process TAS --
GO:0008152 metabolic process --
GO:0015012 heparan sulfate proteoglycan biosynthetic process IEA --
GO:0030203 glycosaminoglycan metabolic process TAS --
genes like me logo Genes that share ontologies with NDST2: view

Transcripts for NDST2 Gene

Unigene Clusters for NDST2 Gene

N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 2:
Representative Sequences:

CRISPR Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for NDST2

Primer Products

  • OriGene qSTAR qPCR primer pairs in human,mouse,rat for NDST2

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for NDST2 Gene

ExUns: 1a · 1b ^ 2a · 2b · 2c ^ 3 ^ 4a · 4b · 4c ^ 5 ^ 6 ^ 7 ^ 8a · 8b ^ 9 ^ 10a · 10b · 10c ^ 11a · 11b ^ 12a · 12b · 12c ^ 13a · 13b
SP1: - - -
SP2: -
SP3: - -
SP4:
SP5:
SP6:
SP7: -

Relevant External Links for NDST2 Gene

GeneLoc Exon Structure for
NDST2
ECgene alternative splicing isoforms for
NDST2

Expression for NDST2 Gene

mRNA expression in normal human tissues for NDST2 Gene

mRNA differential expression in normal tissues according to GTEx for NDST2 Gene

This gene is overexpressed in Whole Blood (4.2).

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, MOPED, and MaxQB for NDST2 Gene

SOURCE GeneReport for Unigene cluster for NDST2 Gene Hs.654758

genes like me logo Genes that share expressions with NDST2: view

In Situ Assay Products

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery and mRNA Expression by UniProt/SwissProt for NDST2 Gene

Orthologs for NDST2 Gene

This gene was present in the common ancestor of animals.

Orthologs for NDST2 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia NDST2 35
  • 99.77 (n)
  • 99.48 (a)
NDST2 36
  • 100 (a)
OneToMany
cow
(Bos Taurus)
Mammalia NDST2 35
  • 93.39 (n)
  • 97.4 (a)
NDST2 36
  • 97 (a)
OneToMany
dog
(Canis familiaris)
Mammalia -- 36
  • 98 (a)
OneToMany
NDST2 35
  • 94.64 (n)
  • 98.07 (a)
mouse
(Mus musculus)
Mammalia Ndst2 35
  • 91.51 (n)
  • 96.72 (a)
Ndst2 16
Ndst2 36
  • 97 (a)
OneToMany
oppossum
(Monodelphis domestica)
Mammalia -- 36
  • 93 (a)
OneToMany
platypus
(Ornithorhynchus anatinus)
Mammalia -- 36
  • 92 (a)
OneToMany
rat
(Rattus norvegicus)
Mammalia Ndst2 35
  • 90.18 (n)
  • 96.04 (a)
chicken
(Gallus gallus)
Aves -- 36
  • 82 (a)
OneToMany
NDST2 35
  • 74.15 (n)
  • 82.55 (a)
lizard
(Anolis carolinensis)
Reptilia -- 36
  • 81 (a)
OneToMany
tropical clawed frog
(Silurana tropicalis)
Amphibia ndst2 35
  • 71.08 (n)
  • 79.48 (a)
zebrafish
(Danio rerio)
Actinopterygii ndst2a 35
  • 70.77 (n)
  • 76.08 (a)
ndst2a 36
  • 73 (a)
ManyToMany
ndst2a 36
  • 75 (a)
ManyToMany
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP006328 35
  • 59.12 (n)
  • 58.36 (a)
fruit fly
(Drosophila melanogaster)
Insecta sfl 35
  • 56.65 (n)
  • 56.07 (a)
sfl 36
  • 46 (a)
OneToMany
sfl 37
  • 54 (a)
worm
(Caenorhabditis elegans)
Secernentea hst-1 35
  • 49.28 (n)
  • 45.37 (a)
hst-1 36
  • 42 (a)
OneToMany
hst-1 37
  • 43 (a)
sea squirt
(Ciona savignyi)
Ascidiacea -- 36
  • 51 (a)
OneToMany
Species with no ortholog for NDST2:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for NDST2 Gene

ENSEMBL:
Gene Tree for NDST2 (if available)
TreeFam:
Gene Tree for NDST2 (if available)

Paralogs for NDST2 Gene

Selected SIMAP similar genes for NDST2 Gene using alignment to 5 proteins:

genes like me logo Genes that share paralogs with NDST2: view

Variants for NDST2 Gene

Sequence variations from dbSNP and Humsavar for NDST2 Gene

SNP ID Clin Chr 10 pos Sequence Context AA Info Type MAF
rs2457442 -- 73,804,363(+) Aggcc(A/G)ggtgc intron-variant
rs2459447 -- 73,804,366(+) ccagg(C/T)gcggt intron-variant
rs2459448 -- 73,804,577(+) ggttg(C/T)agtga intron-variant
rs3758491 -- 73,808,474(+) GGAAG(A/G)GTAGA utr-variant-5-prime, upstream-variant-2KB
rs3758492 -- 73,808,487(+) GATAG(A/G)AAAAT utr-variant-5-prime, upstream-variant-2KB

Structural Variations from Database of Genomic Variants (DGV) for NDST2 Gene

Variant ID Type Subtype PubMed ID
nsv831912 CNV Gain 17160897
nsv825468 CNV Gain 20364138
dgv46n21 CNV Loss 19592680

Relevant External Links for NDST2 Gene

HapMap Linkage Disequilibrium report
NDST2

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for NDST2 Gene

Disorders for NDST2 Gene

Relevant External Links for NDST2

Genetic Association Database (GAD)
NDST2
Human Genome Epidemiology (HuGE) Navigator
NDST2
genes like me logo Genes that share disorders with NDST2: view

No data available for UniProtKB/Swiss-Prot for NDST2 Gene

Publications for NDST2 Gene

  1. Characterization of the N-deacetylase domain from the heparan sulfate N-deacetylase/N-sulfotransferase 2. (PMID: 16343444) Duncan M.B. … Liu J. (Biochem. Biophys. Res. Commun. 2006) 3 4 23
  2. cDNA cloning, genomic organization and chromosomal localization of human heparan glucosaminyl N-deacetylase/N-sulphotransferase-2. (PMID: 9601056) Humphries D.E. … Karlinsky J.B. (Biochem. J. 1998) 2 3 4
  3. The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC). (PMID: 15489334) Gerhard D.S. … Malek J. (Genome Res. 2004) 3 4
  4. A scan of chromosome 10 identifies a novel locus showing strong association with late-onset Alzheimer disease. (PMID: 16385451) Grupe A. … Goate A. (Am. J. Hum. Genet. 2006) 3 48
  5. Influence of high glucose concentrations on the expression of glycosaminoglycans and N-deacetylase/N-sulphotransferase mRNA in cultured skin fibroblasts from diabetic patients with or without nephropathy. (PMID: 11865082) Yard B. … van Der Woude F. (Nephrol. Dial. Transplant. 2002) 3 23

Products for NDST2 Gene

Sources for NDST2 Gene

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