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Aliases for NDST2 Gene

Aliases for NDST2 Gene

  • N-Deacetylase/N-Sulfotransferase 2 2 3
  • N-Deacetylase/N-Sulfotransferase (Heparan Glucosaminyl) 2 2 3 5
  • Glucosaminyl N-Deacetylase/N-Sulfotransferase 2 3 4
  • N-Heparan Sulfate Sulfotransferase 2 3 4
  • N-HSST 2 3 4
  • NDST-2 3 4
  • HSST2 3 4
  • EC 2.8.2.8 4
  • NST2 3

External Ids for NDST2 Gene

Previous GeneCards Identifiers for NDST2 Gene

  • GC10M074340
  • GC10M074665
  • GC10M075454
  • GC10M074906
  • GC10M075231
  • GC10M075561
  • GC10M069557

Summaries for NDST2 Gene

Entrez Gene Summary for NDST2 Gene

  • This gene encodes a member of the N-deacetylase/N-sulfotransferase subfamily of the sulfotransferase 1 proteins. The encoded enzyme has dual functions in processing glucosamine and heparin polymers, including N-deacetylation and N-sulfation. The encoded protein may be localized to the Golgi. [provided by RefSeq, Feb 2009]

GeneCards Summary for NDST2 Gene

NDST2 (N-Deacetylase/N-Sulfotransferase 2) is a Protein Coding gene. Among its related pathways are Metabolism and heparan sulfate biosynthesis. GO annotations related to this gene include hydrolase activity and [heparan sulfate]-glucosamine N-sulfotransferase activity. An important paralog of this gene is NDST4.

UniProtKB/Swiss-Prot for NDST2 Gene

  • Essential bifunctional enzyme that catalyzes both the N-deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA disaccharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis. Plays a role in determining the extent and pattern of sulfation of heparan sulfate. Required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413).

Gene Wiki entry for NDST2 Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for NDST2 Gene

Genomics for NDST2 Gene

Regulatory Elements for NDST2 Gene

Promoters for NDST2 Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters

ENSRs around NDST2 on UCSC Golden Path with GeneCards custom track

Genomic Location for NDST2 Gene

Chromosome:
10
Start:
73,801,911 bp from pter
End:
73,811,831 bp from pter
Size:
9,921 bases
Orientation:
Minus strand

Genomic View for NDST2 Gene

Genes around NDST2 on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
NDST2 Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for NDST2 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for NDST2 Gene

Proteins for NDST2 Gene

  • Protein details for NDST2 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P52849-NDST2_HUMAN
    Recommended name:
    Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 2
    Protein Accession:
    P52849
    Secondary Accessions:
    • Q2TB32
    • Q59H89

    Protein attributes for NDST2 Gene

    Size:
    883 amino acids
    Molecular mass:
    100875 Da
    Quaternary structure:
    • Monomer.
    Miscellaneous:
    • The presence of 4 different heparan sulfate N-deacetylase/N-sulfotransferase enzymes in mammals, as well as differences in their enzyme activity suggest that some initiate heparan sulfate modification/sulfation reactions, whereas other later on fill in or extend already modified heparan sulfate sequences.

    Alternative splice isoforms for NDST2 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for NDST2 Gene

Proteomics data for NDST2 Gene at MOPED

Post-translational modifications for NDST2 Gene

  • Glycosylation at Asn 233, Asn 350, Asn 400, Asn 666, Asn 726, and Asn 802
  • Modification sites at PhosphoSitePlus

Other Protein References for NDST2 Gene

ENSEMBL proteins:
REFSEQ proteins:

No data available for DME Specific Peptides for NDST2 Gene

Domains & Families for NDST2 Gene

Gene Families for NDST2 Gene

Protein Domains for NDST2 Gene

Graphical View of Domain Structure for InterPro Entry

P52849

UniProtKB/Swiss-Prot:

NDST2_HUMAN :
  • Belongs to the sulfotransferase 1 family. NDST subfamily.
Family:
  • Belongs to the sulfotransferase 1 family. NDST subfamily.
genes like me logo Genes that share domains with NDST2: view

Function for NDST2 Gene

Molecular function for NDST2 Gene

GENATLAS Biochemistry:
heparan sulfate-N-deacetylase/N-sulfotransferase 2,with two alternative forms,predominantly expressed in heart,liver,pancreas,located to the trans-Golgi network
UniProtKB/Swiss-Prot BiophysicochemicalProperties:
Kinetic parameters: KM=4.7 uM for K5 polysaccharide {ECO:0000269 PubMed:12634318}; KM=0.76 uM for N-acetylated HS-II {ECO:0000269 PubMed:12634318};
UniProtKB/Swiss-Prot CatalyticActivity:
3-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine = adenosine 3,5-bisphosphate + [heparan sulfate]-N-sulfoglucosamine.
UniProtKB/Swiss-Prot Function:
Essential bifunctional enzyme that catalyzes both the N-deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA disaccharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis. Plays a role in determining the extent and pattern of sulfation of heparan sulfate. Required for the exosomal release of SDCBP, CD63 and syndecan (PubMed:22660413).

Enzyme Numbers (IUBMB) for NDST2 Gene

Gene Ontology (GO) - Molecular Function for NDST2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0050119 N-acetylglucosamine deacetylase activity TAS --
genes like me logo Genes that share ontologies with NDST2: view
genes like me logo Genes that share phenotypes with NDST2: view

Animal Models for NDST2 Gene

MGI Knock Outs for NDST2:

Animal Model Products

No data available for Human Phenotype Ontology , miRNA , Transcription Factor Targets and HOMER Transcription for NDST2 Gene

Localization for NDST2 Gene

Subcellular locations from UniProtKB/Swiss-Prot for NDST2 Gene

Golgi apparatus membrane; Single-pass type II membrane protein.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for NDST2 Gene COMPARTMENTS Subcellular localization image for NDST2 gene
Compartment Confidence
golgi apparatus 5
cytosol 2
extracellular 2
endoplasmic reticulum 1
lysosome 1
vacuole 1

No data available for Gene Ontology (GO) - Cellular Components for NDST2 Gene

Pathways & Interactions for NDST2 Gene

genes like me logo Genes that share pathways with NDST2: view

UniProtKB/Swiss-Prot P52849-NDST2_HUMAN

  • Pathway: Glycan metabolism; heparan sulfate biosynthesis.
  • Pathway: Glycan metabolism; heparin biosynthesis.

Gene Ontology (GO) - Biological Process for NDST2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0030203 glycosaminoglycan metabolic process TAS --
GO:0030210 heparin biosynthetic process IEA --
GO:0044281 small molecule metabolic process TAS --
genes like me logo Genes that share ontologies with NDST2: view

No data available for SIGNOR curated interactions for NDST2 Gene

Drugs & Compounds for NDST2 Gene

(2) Additional Compounds for NDST2 Gene - From: HMDB

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
Adenosine 3',5'-diphosphate
  • 3'-Phosphoryl-AMP
  • 3,5-ADP
  • 3,5-Diphosphoadenosine
  • 3-Phosphoadenosine 5-phosphate
  • 5-(Dihydrogen phosphate) 3-Adenylate
1053-73-2
Phosphoadenosine phosphosulfate
  • 3'-Phospho-5'-adenylyl sulfate
  • 3'-Phospho-5'-adenylyl sulphate
  • 3'-Phosphoadenosine 5'-phosphosulfate
  • 3'-Phosphoadenosine 5'-phosphosulphate
  • 3'-Phosphoadenosine-5'-phosphosulfate
482-67-7
genes like me logo Genes that share compounds with NDST2: view

Transcripts for NDST2 Gene

Unigene Clusters for NDST2 Gene

N-deacetylase/N-sulfotransferase (heparan glucosaminyl) 2:
Representative Sequences:

Alternative Splicing Database (ASD) splice patterns (SP) for NDST2 Gene

ExUns: 1a · 1b ^ 2a · 2b · 2c ^ 3 ^ 4a · 4b · 4c ^ 5 ^ 6 ^ 7 ^ 8a · 8b ^ 9 ^ 10a · 10b · 10c ^ 11a · 11b ^ 12a · 12b · 12c ^ 13a · 13b
SP1: - - -
SP2: -
SP3: - -
SP4:
SP5:
SP6:
SP7: -

Relevant External Links for NDST2 Gene

GeneLoc Exon Structure for
NDST2
ECgene alternative splicing isoforms for
NDST2

Expression for NDST2 Gene

mRNA expression in normal human tissues for NDST2 Gene

mRNA differential expression in normal tissues according to GTEx for NDST2 Gene

This gene is overexpressed in Whole Blood (x4.2).

Protein differential expression in normal tissues from HIPED for NDST2 Gene

This gene is overexpressed in Bone marrow mesenchymal stem cell (49.2), Adipocyte (10.8), and Spleen (9.0).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MOPED, and MaxQB for NDST2 Gene



SOURCE GeneReport for Unigene cluster for NDST2 Gene Hs.654758

genes like me logo Genes that share expression patterns with NDST2: view

Protein tissue co-expression partners for NDST2 Gene

Primer Products

In Situ Assay Products

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery and mRNA Expression by UniProt/SwissProt for NDST2 Gene

Orthologs for NDST2 Gene

This gene was present in the common ancestor of animals.

Orthologs for NDST2 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia NDST2 36
  • 100 (a)
OneToMany
NDST2 35
  • 99.77 (n)
  • 99.48 (a)
cow
(Bos Taurus)
Mammalia NDST2 35
  • 93.39 (n)
  • 97.4 (a)
NDST2 36
  • 97 (a)
OneToMany
dog
(Canis familiaris)
Mammalia -- 36
  • 98 (a)
OneToMany
NDST2 35
  • 94.64 (n)
  • 98.07 (a)
mouse
(Mus musculus)
Mammalia Ndst2 16
Ndst2 36
  • 97 (a)
OneToMany
Ndst2 35
  • 91.51 (n)
  • 96.72 (a)
oppossum
(Monodelphis domestica)
Mammalia -- 36
  • 93 (a)
OneToMany
platypus
(Ornithorhynchus anatinus)
Mammalia -- 36
  • 92 (a)
OneToMany
rat
(Rattus norvegicus)
Mammalia Ndst2 35
  • 90.18 (n)
  • 96.04 (a)
chicken
(Gallus gallus)
Aves NDST2 35
  • 74.15 (n)
  • 82.55 (a)
-- 36
  • 82 (a)
OneToMany
lizard
(Anolis carolinensis)
Reptilia -- 36
  • 81 (a)
OneToMany
tropical clawed frog
(Silurana tropicalis)
Amphibia ndst2 35
  • 71.08 (n)
  • 79.48 (a)
zebrafish
(Danio rerio)
Actinopterygii ndst2a 36
  • 73 (a)
ManyToMany
ndst2a 36
  • 75 (a)
ManyToMany
ndst2a 35
  • 70.77 (n)
  • 76.08 (a)
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP006328 35
  • 59.12 (n)
  • 58.36 (a)
fruit fly
(Drosophila melanogaster)
Insecta sfl 35
  • 56.65 (n)
  • 56.07 (a)
sfl 37
  • 54 (a)
sfl 36
  • 46 (a)
OneToMany
worm
(Caenorhabditis elegans)
Secernentea hst-1 35
  • 49.28 (n)
  • 45.37 (a)
hst-1 36
  • 42 (a)
OneToMany
hst-1 37
  • 43 (a)
sea squirt
(Ciona savignyi)
Ascidiacea -- 36
  • 51 (a)
OneToMany
Species with no ortholog for NDST2:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for NDST2 Gene

ENSEMBL:
Gene Tree for NDST2 (if available)
TreeFam:
Gene Tree for NDST2 (if available)

Paralogs for NDST2 Gene

(6) SIMAP similar genes for NDST2 Gene using alignment to 5 proteins:

genes like me logo Genes that share paralogs with NDST2: view

Variants for NDST2 Gene

Sequence variations from dbSNP and Humsavar for NDST2 Gene

SNP ID Clin Chr 10 pos Sequence Context AA Info Type
rs4746150 -- 73,806,177(+) CCCCA(A/G)TTATG intron-variant
rs3812637 -- 73,809,088(-) TGCTC(A/G)GTCAT intron-variant, upstream-variant-2KB, utr-variant-5-prime
rs3841208 -- 73,809,001(-) TTCCT(-/TTCT)ATCAT intron-variant, upstream-variant-2KB, utr-variant-5-prime
rs11000781 -- 73,803,485(+) CTCAA(A/G)CCTCA intron-variant, downstream-variant-500B
rs11000782 -- 73,804,248(+) CCACA(A/G)ATGAT intron-variant

Structural Variations from Database of Genomic Variants (DGV) for NDST2 Gene

Variant ID Type Subtype PubMed ID
nsv831912 CNV Gain 17160897
nsv825468 CNV Gain 20364138
dgv46n21 CNV Loss 19592680

Variation tolerance for NDST2 Gene

Residual Variation Intolerance Score: 17.3% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 2.47; 43.35% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for NDST2 Gene

HapMap Linkage Disequilibrium report
NDST2

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for NDST2 Gene

Disorders for NDST2 Gene

Relevant External Links for NDST2

Genetic Association Database (GAD)
NDST2
Human Genome Epidemiology (HuGE) Navigator
NDST2
Atlas of Genetics and Cytogenetics in Oncology and Haematology:
NDST2

No disorders were found for NDST2 Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for NDST2 Gene

Publications for NDST2 Gene

  1. cDNA cloning, genomic organization and chromosomal localization of human heparan glucosaminyl N-deacetylase/N-sulphotransferase-2. (PMID: 9601056) Humphries D.E. … Karlinsky J.B. (Biochem. J. 1998) 2 3 4 67
  2. Characterization of the N-deacetylase domain from the heparan sulfate N-deacetylase/N-sulfotransferase 2. (PMID: 16343444) Duncan M.B. … Liu J. (Biochem. Biophys. Res. Commun. 2006) 3 23
  3. Influence of high glucose concentrations on the expression of glycosaminoglycans and N-deacetylase/N-sulphotransferase mRNA in cultured skin fibroblasts from diabetic patients with or without nephropathy. (PMID: 11865082) Yard B. … van Der Woude F. (Nephrol. Dial. Transplant. 2002) 3 23
  4. The BioPlex Network: A Systematic Exploration of the Human Interactome. (PMID: 26186194) Huttlin E.L. … Gygi S.P. (Cell 2015) 3
  5. Synthesis of heparan sulfate with cyclophilin B-binding properties is determined by cell type-specific expression of sulfotransferases. (PMID: 19940140) Deligny A. … Allain F. (J. Biol. Chem. 2010) 23

Products for NDST2 Gene

Sources for NDST2 Gene

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