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Aliases for NDFIP1 Gene

Aliases for NDFIP1 Gene

  • Nedd4 Family Interacting Protein 1 2 3
  • N4WBP5 3 4 6
  • Putative NF-Kappa-B-Activating Protein 164 3 4
  • Putative NFKB And MAPK-Activating Protein 3 4
  • Breast Cancer-Associated Protein SGA-1M 3 4
  • Putative MAPK-Activating Protein PM13 3 4
  • NEDD4 Family-Interacting Protein 1 3
  • Nedd4 WW Domain-Binding Protein 5 3
  • NEDD4 WW Domain-Binding Protein 5 4

External Ids for NDFIP1 Gene

Previous GeneCards Identifiers for NDFIP1 Gene

  • GC05P142071
  • GC05P141471
  • GC05P141516
  • GC05P141468
  • GC05P136635
  • GC05P141489

Summaries for NDFIP1 Gene

Entrez Gene Summary for NDFIP1 Gene

  • The protein encoded by this gene belongs to a small group of evolutionarily conserved proteins with three transmembrane domains. It is a potential target for ubiquitination by the Nedd4 family of proteins. This protein is thought to be part of a family of integral Golgi membrane proteins. [provided by RefSeq, Jul 2008]

GeneCards Summary for NDFIP1 Gene

NDFIP1 (Nedd4 Family Interacting Protein 1) is a Protein Coding gene. Diseases associated with NDFIP1 include breast cancer. GO annotations related to this gene include signal transducer activity and WW domain binding. An important paralog of this gene is NDFIP2.

UniProtKB/Swiss-Prot for NDFIP1 Gene

  • Activates HECT domain-containing E3 ubiquitin-protein ligases, including NEDD4 and ITCH, and consequently modulates the stability of their targets. As a result, controls many cellular processes. Prevents chronic T-helper cells-mediated inflammation by activating ITCH and thus controlling JUNB degradation (By similarity). In cortical neurons, mediates the ubiquitination of SLC11A2/DMT1 by NEDD4L, leading to down-regulation of the divalent metal transporter and protection of the cells from cobalt and iron toxicity. Modulates EGFR signaling through multiple pathways. In particular, may regulate the ratio of AKT1-to-MAPK8 signaling in response to EGF, acting on AKT1 probably through PTEN destabilization and on MAPK8 through ITCH-dependent MAP2K4 inactivation. As a result, may control cell growth rate.

No data available for Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for NDFIP1 Gene

Genomics for NDFIP1 Gene

Regulatory Elements for NDFIP1 Gene

Epigenetics Products

  • DNA Methylation CpG Assay Predesigned for Pyrosequencing in human,mouse,rat

Genomic Location for NDFIP1 Gene

Start:
142,108,505 bp from pter
End:
142,154,443 bp from pter
Size:
45,939 bases
Orientation:
Plus strand

Genomic View for NDFIP1 Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for NDFIP1 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for NDFIP1 Gene

Proteins for NDFIP1 Gene

  • Protein details for NDFIP1 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Q9BT67-NFIP1_HUMAN
    Recommended name:
    NEDD4 family-interacting protein 1
    Protein Accession:
    Q9BT67
    Secondary Accessions:
    • B2RDB8
    • D3DQF0
    • Q658T8
    • Q8N2E3
    • Q8N2F9

    Protein attributes for NDFIP1 Gene

    Size:
    221 amino acids
    Molecular mass:
    24899 Da
    Quaternary structure:
    • Forms heterodimers with NDFIP2. Interacts with several E3 ubiquitin-protein ligases, including NEDD4, NEDD4L and WWP2. Interacts with ITCH, U2SURP and WWP2 (By similarity). The interaction with NEDD4, NEDD4L and ITCH leads to relocalization of these proteins to exosomes and eventually to exosomal secretion. Interacts with SLC11A2/DMT1. Interacts with PTEN. May interact with phosphorylated EGFR.

    Alternative splice isoforms for NDFIP1 Gene

    UniProtKB/Swiss-Prot:

neXtProt entry for NDFIP1 Gene

Proteomics data for NDFIP1 Gene at MOPED

Post-translational modifications for NDFIP1 Gene

  • Ubiquitinated by NEDD4 and ITCH; mono-, di- and polyubiquitinated forms are detected. Ubiquitination regulates its degradation.
  • Undergoes transient tyrosine phosphorylation following EGF stimulation, most probably by catalyzed by SRC. Phosphorylation SRC is enhanced in the presence of NDFIP2 which may act as a scaffold to recruit SRC to NDFIP1.
  • Ubiquitination at Lys63 and Lys83
  • Modification sites at PhosphoSitePlus

Other Protein References for NDFIP1 Gene

ENSEMBL proteins:
REFSEQ proteins:

No data available for DME Specific Peptides for NDFIP1 Gene

Domains for NDFIP1 Gene

Protein Domains for NDFIP1 Gene

InterPro:
ProtoNet:

Suggested Antigen Peptide Sequences for NDFIP1 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry

Q9BT67

UniProtKB/Swiss-Prot:

NFIP1_HUMAN :
  • Q9BT67
Domain:
  • The PY/WW-binding motifs are required for E3 ubiquitin-protein ligase binding and activation and for ubiquitination
genes like me logo Genes that share domains with NDFIP1: view

No data available for Gene Families for NDFIP1 Gene

Function for NDFIP1 Gene

Molecular function for NDFIP1 Gene

UniProtKB/Swiss-Prot Function: Activates HECT domain-containing E3 ubiquitin-protein ligases, including NEDD4 and ITCH, and consequently modulates the stability of their targets. As a result, controls many cellular processes. Prevents chronic T-helper cells-mediated inflammation by activating ITCH and thus controlling JUNB degradation (By similarity). In cortical neurons, mediates the ubiquitination of SLC11A2/DMT1 by NEDD4L, leading to down-regulation of the divalent metal transporter and protection of the cells from cobalt and iron toxicity. Modulates EGFR signaling through multiple pathways. In particular, may regulate the ratio of AKT1-to-MAPK8 signaling in response to EGF, acting on AKT1 probably through PTEN destabilization and on MAPK8 through ITCH-dependent MAP2K4 inactivation. As a result, may control cell growth rate.
UniProtKB/Swiss-Prot Induction: Increased protein expression in neuronal cells in response to Co(2+) or Fe(2+) ions.

Gene Ontology (GO) - Molecular Function for NDFIP1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0004871 signal transducer activity IMP 12761501
GO:0005515 protein binding IPI 18776082
GO:0050699 WW domain binding IEA --
genes like me logo Genes that share ontologies with NDFIP1: view
genes like me logo Genes that share phenotypes with NDFIP1: view

Animal Model Products

CRISPR Products

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for NDFIP1

In Situ Assay Products

Flow Cytometry Products

No data available for Enzyme Numbers (IUBMB) , Animal Models , Transcription Factor Targeting and HOMER Transcription for NDFIP1 Gene

Localization for NDFIP1 Gene

Subcellular locations from UniProtKB/Swiss-Prot for NDFIP1 Gene

Endosome membrane; Multi-pass membrane protein. Golgi apparatus membrane. Secreted. Note=Detected in exosomes and secreted via the exosomal pathway.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for NDFIP1 Gene COMPARTMENTS Subcellular localization image for NDFIP1 gene
Compartment Confidence
endosome 5
extracellular 5
golgi apparatus 2
plasma membrane 2
lysosome 1
vacuole 1

Gene Ontology (GO) - Cellular Components for NDFIP1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000139 Golgi membrane IEA --
GO:0005576 extracellular region IEA --
GO:0005768 endosome --
GO:0005794 Golgi apparatus --
GO:0005938 cell cortex IEA --
genes like me logo Genes that share ontologies with NDFIP1: view

Pathways for NDFIP1 Gene

SuperPathways for NDFIP1 Gene

No Data Available

Gene Ontology (GO) - Biological Process for NDFIP1 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0002761 regulation of myeloid leukocyte differentiation IEA --
GO:0002829 negative regulation of type 2 immune response IEA --
GO:0006879 cellular iron ion homeostasis IMP 18776082
GO:0007165 signal transduction IMP 12761501
GO:0010629 negative regulation of gene expression IMP 18776082
genes like me logo Genes that share ontologies with NDFIP1: view

No data available for Pathways by source for NDFIP1 Gene

Transcripts for NDFIP1 Gene

mRNA/cDNA for NDFIP1 Gene

Unigene Clusters for NDFIP1 Gene

Nedd4 family interacting protein 1:
Representative Sequences:

CRISPR Products

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for NDFIP1

Primer Products

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for NDFIP1 Gene

ExUns: 1a · 1b ^ 2a · 2b ^ 3a · 3b ^ 4a · 4b ^ 5 ^ 6a · 6b ^ 7a · 7b ^ 8a · 8b
SP1: - -
SP2: - -
SP3: - -
SP4:
SP5:

Relevant External Links for NDFIP1 Gene

GeneLoc Exon Structure for
NDFIP1
ECgene alternative splicing isoforms for
NDFIP1

Expression for NDFIP1 Gene

mRNA expression in normal human tissues for NDFIP1 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, MOPED, and MaxQB for NDFIP1 Gene

SOURCE GeneReport for Unigene cluster for NDFIP1 Gene Hs.9788

mRNA Expression by UniProt/SwissProt for NDFIP1 Gene

Q9BT67-NFIP1_HUMAN
Tissue specificity: Widely expressed. Higher levels are detected in cerebellum, pituitary, thalamus, kidney, liver, testis, salivary glands and placenta. Also expressed in fetal brain, kidney and lung.
genes like me logo Genes that share expressions with NDFIP1: view

In Situ Assay Products

No data available for mRNA differential expression in normal tissues for NDFIP1 Gene

Orthologs for NDFIP1 Gene

This gene was present in the common ancestor of animals.

Orthologs for NDFIP1 Gene

Organism Taxonomy Gene Similarity Type Details
chimpanzee
(Pan troglodytes)
Mammalia NDFIP1 35
  • 99.85 (n)
  • 99.55 (a)
NDFIP1 36
  • 100 (a)
OneToOne
cow
(Bos Taurus)
Mammalia NDFIP1 35
  • 95 (n)
  • 96 (a)
NDFIP1 36
  • 90 (a)
OneToOne
dog
(Canis familiaris)
Mammalia NDFIP1 35
  • 95.05 (n)
  • 97.03 (a)
NDFIP1 36
  • 92 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Ndfip1 35
  • 93.36 (n)
  • 97.16 (a)
Ndfip1 16
Ndfip1 36
  • 97 (a)
OneToOne
oppossum
(Monodelphis domestica)
Mammalia NDFIP1 36
  • 93 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia -- 36
  • 72 (a)
ManyToMany
rat
(Rattus norvegicus)
Mammalia Ndfip1 35
  • 92.73 (n)
  • 97.63 (a)
chicken
(Gallus gallus)
Aves -- 36
  • 90 (a)
OneToMany
-- 36
  • 89 (a)
OneToMany
lizard
(Anolis carolinensis)
Reptilia -- 36
  • 63 (a)
OneToMany
-- 36
  • 90 (a)
OneToMany
tropical clawed frog
(Silurana tropicalis)
Amphibia ndfip1 35
  • 83.09 (n)
  • 91.22 (a)
Str.1792 35
zebrafish
(Danio rerio)
Actinopterygii ndfip1 35
  • 73.33 (n)
  • 78.54 (a)
ndfip1 36
  • 77 (a)
OneToMany
ndfip1l 36
  • 77 (a)
OneToMany
wufc17e11 35
African malaria mosquito
(Anopheles gambiae)
Insecta AgaP_AGAP005801 35
  • 46.91 (n)
  • 36.6 (a)
fruit fly
(Drosophila melanogaster)
Insecta Ndfip 35
  • 48.09 (n)
  • 42.62 (a)
Ndfip 36
  • 23 (a)
OneToMany
sea squirt
(Ciona savignyi)
Ascidiacea -- 36
  • 32 (a)
OneToMany
Species with no ortholog for NDFIP1:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for NDFIP1 Gene

ENSEMBL:
Gene Tree for NDFIP1 (if available)
TreeFam:
Gene Tree for NDFIP1 (if available)

Paralogs for NDFIP1 Gene

Paralogs for NDFIP1 Gene

Selected SIMAP similar genes for NDFIP1 Gene using alignment to 1 proteins:

Pseudogenes.org Pseudogenes for NDFIP1 Gene

genes like me logo Genes that share paralogs with NDFIP1: view

Variants for NDFIP1 Gene

Sequence variations from dbSNP and Humsavar for NDFIP1 Gene

SNP ID Clin Chr 05 pos Sequence Context AA Info Type MAF
rs8378 -- 142,153,949(-) AAAGC(G/T)TATCA utr-variant-3-prime
rs14449 -- 142,152,661(+) AGAAA(C/T)CAGTG utr-variant-3-prime
rs166079 -- 142,149,394(-) AGGAA(A/G)ACAAC intron-variant
rs166080 -- 142,148,300(-) GTACA(C/T)GAGCT intron-variant
rs166081 -- 142,146,639(-) CTACA(C/T)CTACA intron-variant

Structural Variations from Database of Genomic Variants (DGV) for NDFIP1 Gene

Variant ID Type Subtype PubMed ID
nsv509994 CNV Loss 20534489
nsv830509 CNV Gain 17160897

Relevant External Links for NDFIP1 Gene

HapMap Linkage Disequilibrium report
NDFIP1

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for NDFIP1 Gene

Disorders for NDFIP1 Gene

MalaCards: The human disease database

MalaCards: The human disease database. (1) Diseases for NDFIP1 Gene including...

Search for NDFIP1 Gene in MalaCards »

Relevant External Links for NDFIP1

Genetic Association Database (GAD)
NDFIP1
Human Genome Epidemiology (HuGE) Navigator
NDFIP1
genes like me logo Genes that share disorders with NDFIP1: view

No data available for UniProtKB/Swiss-Prot for NDFIP1 Gene

Publications for NDFIP1 Gene

  1. Nedd4 family-interacting protein 1 (Ndfip1) is required for the exosomal secretion of Nedd4 family proteins. (PMID: 18819914) Putz U. … Tan S.S. (J. Biol. Chem. 2008) 3 4 23
  2. Control of the activity of WW-HECT domain E3 ubiquitin ligases by NDFIP proteins. (PMID: 19343052) Mund T. … Pelham H.R. (EMBO Rep. 2009) 3 4 23
  3. Divalent metal transporter 1 (DMT1) regulation by Ndfip1 prevents metal toxicity in human neurons. (PMID: 19706893) Howitt J. … Tan S.S. (Proc. Natl. Acad. Sci. U.S.A. 2009) 3 4 23
  4. N4WBP5, a potential target for ubiquitination by the Nedd4 family of proteins, is a novel Golgi-associated protein. (PMID: 11748237) Harvey K.F. … Kumar S. (J. Biol. Chem. 2002) 2 3 4
  5. Identification of multiple proteins expressed in murine embryos as binding partners for the WW domains of the ubiquitin-protein ligase Nedd4. (PMID: 11042109) Jolliffe C.N. … Kumar S. (Biochem. J. 2000) 2 3

Products for NDFIP1 Gene

Sources for NDFIP1 Gene

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