Free for academic non-profit institutions. Other users need a Commercial license

Aliases for NCL Gene

Aliases for NCL Gene

  • Nucleolin 2 3
  • Protein C23 4
  • C23 3

External Ids for NCL Gene

Previous GeneCards Identifiers for NCL Gene

  • GC02M230377
  • GC02M231051
  • GC02M232283
  • GC02M232521
  • GC02M232522
  • GC02M232144
  • GC02M232027
  • GC02M232318
  • GC02M224160

Summaries for NCL Gene

Entrez Gene Summary for NCL Gene

  • Nucleolin (NCL), a eukaryotic nucleolar phosphoprotein, is involved in the synthesis and maturation of ribosomes. It is located mainly in dense fibrillar regions of the nucleolus. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. The intron 11 of the NCL gene encodes a small nucleolar RNA, termed U20. [provided by RefSeq, Jul 2008]

GeneCards Summary for NCL Gene

NCL (Nucleolin) is a Protein Coding gene. Among its related pathways are Translational Control and Development Slit-Robo signaling. GO annotations related to this gene include nucleic acid binding and identical protein binding.

UniProtKB/Swiss-Prot for NCL Gene

  • Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5-UUAGGG-3 repeats more tightly than the telomeric single-stranded DNA 5-TTAGGG-3 repeats.

Gene Wiki entry for NCL Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for NCL Gene

Genomics for NCL Gene

Regulatory Elements for NCL Gene

Transcription factor binding sites by QIAGEN in the NCL gene promoter:

Epigenetics Products

  • DNA Methylation CpG Assay Predesigned for Pyrosequencing in human,mouse,rat

Genomic Location for NCL Gene

Chromosome:
2
Start:
231,453,531 bp from pter
End:
231,483,641 bp from pter
Size:
30,111 bases
Orientation:
Minus strand

Genomic View for NCL Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
GeneLoc Logo Gene Density

RefSeq DNA sequence for NCL Gene

Proteins for NCL Gene

  • Protein details for NCL Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    P19338-NUCL_HUMAN
    Recommended name:
    Nucleolin
    Protein Accession:
    P19338
    Secondary Accessions:
    • Q53SK1
    • Q8NB06
    • Q9UCF0
    • Q9UDG1

    Protein attributes for NCL Gene

    Size:
    710 amino acids
    Molecular mass:
    76614 Da
    Quaternary structure:
    • Identified in a IGF2BP1-dependent mRNP granule complex containing untranslated mRNAs. Interacts with APTX and NSUN2. Component of the SWAP complex that consists of NPM1, NCL/nucleolin, PARP1 and SWAP70. Component of a complex which is at least composed of HTATSF1/Tat-SF1, the P-TEFb complex components CDK9 and CCNT1, RNA polymerase II, SUPT5H, and NCL/nucleolin. Interacts (via RRM1 and C-terminal RRM4/Arg/Gly-rich domains) with TERT; the interaction is important for nucleolar localization of TERT. Interacts with ERBB4. Interacts with GZF1; this interaction is important for nucleolar localization of GZF1. Interacts with NVL and C1QBP. Interacts with AICDA (By similarity). Interacts (via N-terminus domain) with SETX (PubMed:21700224). Interacts with WDR46 (PubMed:23848194).
    SequenceCaution:
    • Sequence=BAC03738.1; Type=Miscellaneous discrepancy; Note=Unlikely isoform. Aberrant splice sites.; Evidence={ECO:0000305};

    Three dimensional structures from OCA and Proteopedia for NCL Gene

neXtProt entry for NCL Gene

Proteomics data for NCL Gene at MOPED

Post-translational modifications for NCL Gene

  • Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group (By similarity).
  • Ubiquitination at Lys 324, Lys 333, Lys 370, Lys 467, Lys 513, Lys 572, and Lys 610
  • Modification sites at PhosphoSitePlus

Other Protein References for NCL Gene

Antibody Products

  • Cell Signaling Technology (CST) Antibodies for NCL (NCL)

No data available for DME Specific Peptides for NCL Gene

Domains & Families for NCL Gene

Gene Families for NCL Gene

Protein Domains for NCL Gene

Suggested Antigen Peptide Sequences for NCL Gene

Graphical View of Domain Structure for InterPro Entry

P19338

UniProtKB/Swiss-Prot:

NUCL_HUMAN :
  • Contains 4 RRM (RNA recognition motif) domains.
Domain:
  • Contains 4 RRM (RNA recognition motif) domains.
genes like me logo Genes that share domains with NCL: view

Function for NCL Gene

Molecular function for NCL Gene

GENATLAS Biochemistry:
nucleolin,major multifunctional nucleolar protein of exponentially growing cells,characterized by unique tripartite function within each domain,performing activities i.e. a specific DNA helicase and DNA-dependent ATPase,also acting as a sequence-specific RNA binding protein an autoantigen,a component of B cell specific transcription factor,involved in ribosome biogenesis,cytokinesis,nucleogenesis,cell proliferation and growth,chromatin remodeling etc...
UniProtKB/Swiss-Prot Function:
Nucleolin is the major nucleolar protein of growing eukaryotic cells. It is found associated with intranucleolar chromatin and pre-ribosomal particles. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly. May play a role in the process of transcriptional elongation. Binds RNA oligonucleotides with 5-UUAGGG-3 repeats more tightly than the telomeric single-stranded DNA 5-TTAGGG-3 repeats.

Gene Ontology (GO) - Molecular Function for NCL Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0000166 nucleotide binding IEA --
GO:0003676 nucleic acid binding --
GO:0003697 single-stranded DNA binding IEA --
GO:0003723 RNA binding IDA 8321232
GO:0005102 receptor binding --
genes like me logo Genes that share ontologies with NCL: view
genes like me logo Genes that share phenotypes with NCL: view

Animal Model Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for NCL

In Situ Assay Products

Flow Cytometry Products

No data available for Enzyme Numbers (IUBMB) , Animal Models , Transcription Factor Targets and HOMER Transcription for NCL Gene

Localization for NCL Gene

Subcellular locations from UniProtKB/Swiss-Prot for NCL Gene

Nucleus, nucleolus. Cytoplasm. Note=Localized in cytoplasmic mRNP granules containing untranslated mRNAs.

Subcellular locations from

COMPARTMENTS
Jensen Localization Image for NCL Gene COMPARTMENTS Subcellular localization image for NCL gene
Compartment Confidence
extracellular 5
nucleus 5
plasma membrane 2
cytoskeleton 1
cytosol 1

Gene Ontology (GO) - Cellular Components for NCL Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0001650 fibrillar center IEA --
GO:0001651 dense fibrillar component IEA --
GO:0005634 nucleus IDA 15121898
GO:0005654 nucleoplasm IDA 23353999
GO:0005730 nucleolus IDA 12944467
genes like me logo Genes that share ontologies with NCL: view

Pathways & Interactions for NCL Gene

genes like me logo Genes that share pathways with NCL: view

Pathways by source for NCL Gene

1 KEGG pathway for NCL Gene
1 GeneGo (Thomson Reuters) pathway for NCL Gene

PCR Array Products

  • Pathway & Disease-focused RT² Profiler PCR Arrays
    • Telomeres & Telomerase in human,mouse,rat

Gene Ontology (GO) - Biological Process for NCL Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0001525 angiogenesis IDA 16403913
GO:0006897 endocytosis IEA --
GO:0007283 spermatogenesis IEA --
GO:0032760 positive regulation of tumor necrosis factor production IEA --
GO:0043066 negative regulation of apoptotic process IEA --
genes like me logo Genes that share ontologies with NCL: view

No data available for SIGNOR curated interactions for NCL Gene

Drugs & Compounds for NCL Gene

(18) Drugs for NCL Gene - From: NovoSeek and DGIdb

Name Status Disease Links Group Role Mechanism of Action Clinical Trials
Heparin Approved, Investigational Pharma Antagonist 672,226
Hydroxyurea Approved Pharma 181
Paclitaxel Approved Pharma Tubulin and Bcl2 inhibitor, Taxanes 2505
Camptothecin Experimental Pharma Topoisomerase I inhibitor,prototypic, DNA topoisomerase inhibitor 196
Nocodazole Experimental Pharma Tubulin production inhibitor,anti-neoplastic agent 0

(15) Additional Compounds for NCL Gene - From: NovoSeek

Name Synonyms Role CAS Number PubChem IDs PubMed IDs
Actinomycin
Actinomycin D
adenylate
Fructosyllysine
heparan sulfate
  • alpha-Idosane
  • Heparan N-sulfate
  • Heparan N-sulphate
  • Heparan sulfate
  • Heparan sulphate
9050-30-0
genes like me logo Genes that share compounds with NCL: view

Transcripts for NCL Gene

Unigene Clusters for NCL Gene

Nucleolin:
Representative Sequences:

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for NCL

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for NCL Gene

ExUns: 1 ^ 2 ^ 3a · 3b · 3c ^ 4 ^ 5 ^ 6a · 6b ^ 7 ^ 8a · 8b · 8c · 8d · 8e ^ 9a · 9b · 9c ^ 10 ^ 11a · 11b ^ 12a · 12b ^ 13a · 13b · 13c ^
SP1: - - - - - - -
SP2: - - - - - - - - - - - - - -
SP3:
SP4: -
SP5: - - - - - - - - - - - - - - - - -
SP6: - - - - - - - - - - -
SP7:
SP8:
SP9:
SP10:
SP11: - - - - -
SP12: - -
SP13: - - -
SP14: -
SP15:
SP16: - - - - - - - - -

ExUns: 14 ^ 15a · 15b ^ 16 ^ 17a · 17b ^ 18a · 18b ^ 19a · 19b · 19c · 19d ^ 20a · 20b · 20c
SP1: - -
SP2:
SP3: -
SP4: - - - - - - - -
SP5:
SP6:
SP7: - - -
SP8: -
SP9:
SP10:
SP11:
SP12:
SP13:
SP14:
SP15:
SP16:

Relevant External Links for NCL Gene

GeneLoc Exon Structure for
NCL
ECgene alternative splicing isoforms for
NCL

Expression for NCL Gene

mRNA expression in normal human tissues for NCL Gene

Protein differential expression in normal tissues from HIPED for NCL Gene

This gene is overexpressed in Tlymphocyte (6.9).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MOPED, and MaxQB for NCL Gene



SOURCE GeneReport for Unigene cluster for NCL Gene Hs.79110

genes like me logo Genes that share expression patterns with NCL: view

Protein tissue co-expression partners for NCL Gene

- Elite partner

Primer Products

In Situ Assay Products

No data available for mRNA expression in embryonic tissues and stem cells from LifeMap Discovery , mRNA differential expression in normal tissues and mRNA Expression by UniProt/SwissProt for NCL Gene

Orthologs for NCL Gene

This gene was present in the common ancestor of animals and fungi.

Orthologs for NCL Gene

Organism Taxonomy Gene Similarity Type Details
cow
(Bos Taurus)
Mammalia NCL 35
  • 88.41 (n)
  • 86.8 (a)
NCL 36
  • 89 (a)
OneToMany
NCL 36
  • 89 (a)
OneToMany
dog
(Canis familiaris)
Mammalia NCL 35
  • 90.4 (n)
  • 92.09 (a)
NCL 36
  • 92 (a)
OneToOne
mouse
(Mus musculus)
Mammalia Ncl 35
  • 87.67 (n)
  • 85.35 (a)
Ncl 16
Ncl 36
  • 82 (a)
OneToOne
chimpanzee
(Pan troglodytes)
Mammalia NCL 35
  • 99.54 (n)
  • 99.08 (a)
NCL 36
  • 100 (a)
OneToOne
rat
(Rattus norvegicus)
Mammalia Ncl 35
  • 88.2 (n)
  • 86.9 (a)
oppossum
(Monodelphis domestica)
Mammalia NCL 36
  • 72 (a)
OneToOne
platypus
(Ornithorhynchus anatinus)
Mammalia NCL 36
  • 73 (a)
OneToOne
chicken
(Gallus gallus)
Aves NCL 36
  • 59 (a)
OneToOne
lizard
(Anolis carolinensis)
Reptilia NCL 36
  • 58 (a)
OneToOne
tropical clawed frog
(Silurana tropicalis)
Amphibia Str.10165 35
African clawed frog
(Xenopus laevis)
Amphibia LOC397919 35
zebrafish
(Danio rerio)
Actinopterygii ncl 36
  • 50 (a)
OneToOne
baker's yeast
(Saccharomyces cerevisiae)
Saccharomycetes NSR1 36
  • 32 (a)
OneToOne
sea squirt
(Ciona savignyi)
Ascidiacea CSA.8423 36
  • 36 (a)
OneToOne
Species with no ortholog for NCL:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • fruit fly (Drosophila melanogaster)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for NCL Gene

ENSEMBL:
Gene Tree for NCL (if available)
TreeFam:
Gene Tree for NCL (if available)

Paralogs for NCL Gene

(4) SIMAP similar genes for NCL Gene using alignment to 8 proteins:

Pseudogenes.org Pseudogenes for NCL Gene

genes like me logo Genes that share paralogs with NCL: view

No data available for Paralogs for NCL Gene

Variants for NCL Gene

Sequence variations from dbSNP and Humsavar for NCL Gene

SNP ID Clin Chr 02 pos Sequence Context AA Info Type MAF
rs1131171 -- 231,461,706(-) GAGGA(A/G/T)GATGA synonymous-codon, reference, missense
rs2442322 -- 231,460,069(+) ACAGT(A/G)GCAGG intron-variant
rs2442323 -- 231,461,248(+) actcc(A/C/T)gcctg intron-variant
rs2442324 -- 231,462,420(+) TCATT(G/T)GGGGC intron-variant
rs2442325 -- 231,463,796(+) CCACC(A/T)CGTGC intron-variant

Structural Variations from Database of Genomic Variants (DGV) for NCL Gene

Variant ID Type Subtype PubMed ID
nsv834567 CNV Gain 17160897
esv270903 CNV Insertion 20981092
nsv819681 CNV Loss 19587683

Variation tolerance for NCL Gene

Residual Variation Intolerance Score: 12.2% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 2.31; 41.24% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for NCL Gene

HapMap Linkage Disequilibrium report
NCL

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for NCL Gene

Disorders for NCL Gene

Relevant External Links for NCL

Atlas of Genetics and Cytogenetics in Oncology and Haematology:
NCL
genes like me logo Genes that share disorders with NCL: view

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for NCL Gene

Publications for NCL Gene

  1. Nucleolin/C23 is a negative regulator of hydrogen peroxide-induced apoptosis in HUVECs. (PMID: 19757191) Zhang B. … Xiao X. (Cell Stress Chaperones 2010) 23 67
  2. AS-1411, a guanosine-rich oligonucleotide aptamer targeting nucleolin for the potential treatment of cancer, including acute myeloid leukemia. (PMID: 20140822) Mongelard F. … Bouvet P. (Curr. Opin. Mol. Ther. 2010) 23 67
  3. A proteomic study of cMyc improvement of CHO culture. (PMID: 20307306) Kuystermans D. … Al-Rubeai M. (BMC Biotechnol. 2010) 23 67
  4. Nucleolin associates with the human cytomegalovirus DNA polymerase accessory subunit UL44 and is necessary for efficient viral replication. (PMID: 20007282) Strang B.L. … Coen D.M. (J. Virol. 2010) 23 67
  5. A fucose-containing O-glycoepitope on bovine and human nucleolin. (PMID: 19023103) Aldi S. … Rosati F. (Glycobiology 2009) 23 67

Products for NCL Gene

Sources for NCL Gene

Back to Top

Content