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Aliases for NCKAP1L Gene

Aliases for NCKAP1L Gene

  • NCK Associated Protein 1 Like 2 3 5
  • Hematopoietic Protein 1 2 3 4
  • HEM1 3 4
  • Membrane-Associated Protein Hem-1 3
  • Membrane-Associated Protein HEM-1 4
  • Nck-Associated Protein 1-Like 3

External Ids for NCKAP1L Gene

Previous HGNC Symbols for NCKAP1L Gene

  • HEM1

Previous GeneCards Identifiers for NCKAP1L Gene

  • GC12P053178
  • GC12P054891
  • GC12P051930

Summaries for NCKAP1L Gene

Entrez Gene Summary for NCKAP1L Gene

  • This gene encodes a member of the HEM family of tissue-specific transmembrane proteins which are highly conserved from invertebrates through mammals. This gene is only expressed in hematopoietic cells. The encoded protein is a part of the Scar/WAVE complex which plays an important role in regulating cell shape in both metazoans and plants. Alternatively spliced transcript variants encoding different isoforms have been found.[provided by RefSeq, May 2010]

GeneCards Summary for NCKAP1L Gene

NCKAP1L (NCK Associated Protein 1 Like) is a Protein Coding gene. Among its related pathways are Immune System and Regulation of actin dynamics for phagocytic cup formation. GO annotations related to this gene include transcription factor activity, sequence-specific DNA binding and protein complex binding. An important paralog of this gene is NCKAP1.

No data available for UniProtKB/Swiss-Prot , Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for NCKAP1L Gene

Genomics for NCKAP1L Gene

Regulatory Elements for NCKAP1L Gene

Enhancers for NCKAP1L Gene
GeneHancer Identifier Enhancer Score Enhancer Sources Gene-Enhancer Score TSS distance (kb) Number of Genes Away Size (kb) Transcription Factor Binding Sites within enhancer Gene Targets for Enhancer
GH12F054439 1 Ensembl ENCODE 10.9 -58.0 -57957 1.1 CTCF ZNF146 ESRRA ZNF384 RAD21 RFX5 ZNF143 SMC3 ZNF654 ZNF362 GTSF1 ITGA5 ZNF385A GPR84 NCKAP1L RNU6-950P COPZ1 LINC01154
GH12F054354 1.9 FANTOM5 Ensembl ENCODE 4.1 -139.7 -139655 6.7 FEZF1 YBX1 YY1 ZNF143 ZNF263 MCM3 SP3 NFYC TBX21 MEF2D SP1 ENSG00000258344 GPR84 ZNF385A LOC100652999 OR10A7 OR6C74 LOC105378250 MAP3K12 ENSG00000258086
GH12F054527 0.6 ENCODE 11.5 +29.6 29591 0.2 SCRT1 CTCF ZNF654 ZNF585B IKZF1 MAX REST RAD21 PHC1P1 PDE1B NCKAP1L MIR3198-2 PPP1R1A ENSG00000257824
GH12F054436 0.6 ENCODE 10.9 -60.3 -60311 1.1 MTA2 IKZF1 JUNB EBF1 TBX21 RELA GTSF1 ZNF385A GPR84 NCKAP1L RNU6-950P COPZ1 LINC01154
- Elite enhancer and/or Elite enhancer-gene association Download GeneHancer data dump

Enhancers around NCKAP1L on UCSC Golden Path with GeneCards custom track

Promoters for NCKAP1L Gene
Ensembl Regulatory Elements (ENSRs) TSS Distance (bp) Size (bp) Binding Sites for Transcription Factors within promoters
ENSR00001025878 689 1601 HDGF PKNOX1 WRNIP1 SIN3A YY1 ZNF121 ZNF143 FOS SP5 ZNF518A

Genomic Location for NCKAP1L Gene

54,497,711 bp from pter
54,548,238 bp from pter
50,528 bases
Plus strand

Genomic View for NCKAP1L Gene

Genes around NCKAP1L on UCSC Golden Path with GeneCards custom track

Cytogenetic band:
NCKAP1L Gene in genomic location: bands according to Ensembl, locations according to GeneLoc (and/or Entrez Gene and/or Ensembl if different)
Genomic Location for NCKAP1L Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for NCKAP1L Gene

Proteins for NCKAP1L Gene

  • Protein details for NCKAP1L Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Recommended name:
    Nck-associated protein 1-like
    Protein Accession:
    Secondary Accessions:
    • B4DUT5
    • Q52LW0

    Protein attributes for NCKAP1L Gene

    1127 amino acids
    Molecular mass:
    128153 Da
    Quaternary structure:
    No Data Available
    • Sequence=AAA35964.1; Type=Erroneous initiation; Evidence={ECO:0000305};

    Alternative splice isoforms for NCKAP1L Gene


neXtProt entry for NCKAP1L Gene

Post-translational modifications for NCKAP1L Gene

  • Ubiquitination at Lys 40, Lys 42, Lys 49, Lys 60, Lys 326, and Lys 349
  • Modification sites at PhosphoSitePlus

Other Protein References for NCKAP1L Gene

No data available for DME Specific Peptides for NCKAP1L Gene

Domains & Families for NCKAP1L Gene

Protein Domains for NCKAP1L Gene


Graphical View of Domain Structure for InterPro Entry



  • Belongs to the HEM-1/HEM-2 family.
  • Belongs to the HEM-1/HEM-2 family.
genes like me logo Genes that share domains with NCKAP1L: view

No data available for Gene Families for NCKAP1L Gene

Function for NCKAP1L Gene

Gene Ontology (GO) - Molecular Function for NCKAP1L Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005096 GTPase activator activity IMP 16417406
GO:0005515 protein binding IPI 16417406
GO:0030295 protein kinase activator activity IMP 16417406
GO:0032403 protein complex binding IDA 16417406
genes like me logo Genes that share ontologies with NCKAP1L: view
genes like me logo Genes that share phenotypes with NCKAP1L: view

Animal Model Products

Inhibitory RNA Products

Flow Cytometry Products

No data available for Molecular function , Enzyme Numbers (IUBMB) , Human Phenotype Ontology , Animal Models , Transcription Factor Targets and HOMER Transcription for NCKAP1L Gene

Localization for NCKAP1L Gene

Subcellular locations from UniProtKB/Swiss-Prot for NCKAP1L Gene

Cell membrane; Single-pass membrane protein; Cytoplasmic side.

Subcellular locations from

Extracellular space Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi Apparatus Nucleus Mitochondrion 0 1 2 3 4 5 Confidence
COMPARTMENTS Subcellular localization image for NCKAP1L gene
Compartment Confidence
plasma membrane 5
extracellular 5
cytosol 5
nucleus 2
cytoskeleton 1
mitochondrion 1
peroxisome 1

Gene Ontology (GO) - Cellular Components for NCKAP1L Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005829 cytosol TAS --
GO:0005886 plasma membrane IEA --
GO:0005887 integral component of plasma membrane NAS 1932118
GO:0016020 membrane IDA,TAS 19946888
GO:0016021 integral component of membrane IEA --
genes like me logo Genes that share ontologies with NCKAP1L: view

Pathways & Interactions for NCKAP1L Gene

genes like me logo Genes that share pathways with NCKAP1L: view

Gene Ontology (GO) - Biological Process for NCKAP1L Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0001782 B cell homeostasis ISS --
GO:0002262 myeloid cell homeostasis ISS --
GO:0006461 protein complex assembly ISS --
GO:0006935 chemotaxis IDA 16417406
GO:0030011 maintenance of cell polarity IMP 16417406
genes like me logo Genes that share ontologies with NCKAP1L: view

No data available for SIGNOR curated interactions for NCKAP1L Gene

Transcripts for NCKAP1L Gene

Unigene Clusters for NCKAP1L Gene

NCK-associated protein 1-like:
Representative Sequences:

Inhibitory RNA Products

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for NCKAP1L Gene

ExUns: 1a · 1b ^ 2 ^ 3a · 3b ^ 4 ^ 5 ^ 6 ^ 7 ^ 8 ^ 9 ^ 10 ^ 11 ^ 12 ^ 13 ^ 14 ^ 15 ^ 16a · 16b ^ 17 ^ 18a · 18b ^ 19 ^ 20 ^ 21a · 21b ^
SP1: - - -

ExUns: 22a · 22b ^ 23 ^ 24 ^ 25 ^ 26 ^ 27a · 27b ^ 28 ^ 29a · 29b ^ 30 ^ 31 ^ 32
SP1: - -

Relevant External Links for NCKAP1L Gene

GeneLoc Exon Structure for
ECgene alternative splicing isoforms for

Expression for NCKAP1L Gene

mRNA expression in normal human tissues from GTEx, Illumina, BioGPS, and SAGE for NCKAP1L Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for NCKAP1L Gene

This gene is overexpressed in Whole Blood (x13.2) and Spleen (x4.3).

Protein differential expression in normal tissues from HIPED for NCKAP1L Gene

This gene is overexpressed in Lymph node (25.4) and Peripheral blood mononuclear cells (10.2).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MaxQB, and MOPED for NCKAP1L Gene

Protein tissue co-expression partners for NCKAP1L Gene

- Elite partner

NURSA nuclear receptor signaling pathways regulating expression of NCKAP1L Gene:


SOURCE GeneReport for Unigene cluster for NCKAP1L Gene:


mRNA Expression by UniProt/SwissProt for NCKAP1L Gene:

Tissue specificity: Expressed only in cells of hematopoietic origin.
genes like me logo Genes that share expression patterns with NCKAP1L: view

Primer Products

Orthologs for NCKAP1L Gene

This gene was present in the common ancestor of animals.

Orthologs for NCKAP1L Gene

Organism Taxonomy Gene Similarity Type Details
(Pan troglodytes)
Mammalia NCKAP1L 34 35
  • 99.54 (n)
(Canis familiaris)
Mammalia NCKAP1L 34 35
  • 91.92 (n)
(Bos Taurus)
Mammalia NCKAP1L 34 35
  • 91.42 (n)
(Mus musculus)
Mammalia Nckap1l 34 16 35
  • 89.68 (n)
(Rattus norvegicus)
Mammalia Nckap1l 34
  • 88.99 (n)
(Monodelphis domestica)
Mammalia NCKAP1L 35
  • 86 (a)
(Ornithorhynchus anatinus)
Mammalia -- 35
  • 84 (a)
-- 35
  • 78 (a)
(Gallus gallus)
Aves NCKAP1L 34 35
  • 71.13 (n)
(Anolis carolinensis)
Reptilia -- 35
  • 71 (a)
-- 35
  • 68 (a)
tropical clawed frog
(Silurana tropicalis)
Amphibia nckap1l 34
  • 68.63 (n)
(Danio rerio)
Actinopterygii zgc:172352 34
  • 64.13 (n)
  • 60 (a)
Dr.26103 34
fruit fly
(Drosophila melanogaster)
Insecta Hem 35
  • 47 (a)
(Caenorhabditis elegans)
Secernentea gex-3 35
  • 36 (a)
sea squirt
(Ciona savignyi)
Ascidiacea CSA.9782 35
  • 47 (a)
Species where no ortholog for NCKAP1L was found in the sources mined by GeneCards:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for NCKAP1L Gene

Gene Tree for NCKAP1L (if available)
Gene Tree for NCKAP1L (if available)

Paralogs for NCKAP1L Gene

Paralogs for NCKAP1L Gene

(1) SIMAP similar genes for NCKAP1L Gene using alignment to 5 proteins:

genes like me logo Genes that share paralogs with NCKAP1L: view

Variants for NCKAP1L Gene

Sequence variations from dbSNP and Humsavar for NCKAP1L Gene

SNP ID Clin Chr 12 pos Sequence Context AA Info Type
rs10506332 -- 54,533,464(-) ATGAC(C/G)GTTTG intron-variant
rs10528777 -- 54,498,913(+) TTTTA(-/TATT/TATTTATT)TATTT intron-variant
rs10686138 -- 54,519,430(+) TTTTT(-/T)AAAGA intron-variant
rs10747697 -- 54,531,966(+) GGGAG(A/C)GTAAT intron-variant
rs10876565 -- 54,534,262(+) TATAG(A/G)TGGGA intron-variant

Structural Variations from Database of Genomic Variants (DGV) for NCKAP1L Gene

Variant ID Type Subtype PubMed ID
nsv832421 CNV gain 17160897
nsv525674 CNV loss 19592680
nsv508674 CNV deletion 20534489
esv3391984 CNV insertion 20981092

Variation tolerance for NCKAP1L Gene

Residual Variation Intolerance Score: 5.97% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 5.31; 70.55% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for NCKAP1L Gene

SNPedia medical, phenotypic, and genealogical associations of SNPs for

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for NCKAP1L Gene

Disorders for NCKAP1L Gene

Relevant External Links for NCKAP1L

Atlas of Genetics and Cytogenetics in Oncology and Haematology:

No disorders were found for NCKAP1L Gene.

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for NCKAP1L Gene

Publications for NCKAP1L Gene

  1. Hem-1 complexes are essential for Rac activation, actin polymerization, and myosin regulation during neutrophil chemotaxis. (PMID: 16417406) Weiner O.D. … Kirschner M.W. (PLoS Biol. 2006) 3 22 64
  2. The HEM proteins: a novel family of tissue-specific transmembrane proteins expressed from invertebrates through mammals with an essential function in oogenesis. (PMID: 7643388) Baumgartner S. … Hromas R. (J. Mol. Biol. 1995) 3 4 64
  3. Hem-1, a potential membrane protein, with expression restricted to blood cells. (PMID: 1932118) Hromas R. … Kaushansky K. (Biochim. Biophys. Acta 1991) 2 3 64
  4. Panorama of ancient metazoan macromolecular complexes. (PMID: 26344197) Wan C. … Emili A. (Nature 2015) 3 64
  5. An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome. (PMID: 24275569) Bian Y. … Zou H. (J. Proteomics 2014) 4 64

Products for NCKAP1L Gene

Sources for NCKAP1L Gene

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