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Aliases for NCKAP1L Gene

Aliases for NCKAP1L Gene

  • NCK-Associated Protein 1-Like 2 3
  • Hematopoietic Protein 1 2 3 4
  • HEM1 3 4
  • Membrane-Associated Protein Hem-1 3
  • Membrane-Associated Protein HEM-1 4

External Ids for NCKAP1L Gene

Previous HGNC Symbols for NCKAP1L Gene

  • HEM1

Previous GeneCards Identifiers for NCKAP1L Gene

  • GC12P053178
  • GC12P054891
  • GC12P051930

Summaries for NCKAP1L Gene

Entrez Gene Summary for NCKAP1L Gene

  • This gene encodes a member of the HEM family of tissue-specific transmembrane proteins which are highly conserved from invertebrates through mammals. This gene is only expressed in hematopoietic cells. The encoded protein is a part of the Scar/WAVE complex which plays an important role in regulating cell shape in both metazoans and plants. Alternatively spliced transcript variants encoding different isoforms have been found.[provided by RefSeq, May 2010]

GeneCards Summary for NCKAP1L Gene

NCKAP1L (NCK-Associated Protein 1-Like) is a Protein Coding gene. Among its related pathways are Immune System and Signaling by GPCR. GO annotations related to this gene include transcription factor activity, sequence-specific DNA binding and protein complex binding. An important paralog of this gene is NCKAP1.

No data available for UniProtKB/Swiss-Prot , Tocris Summary , Gene Wiki entry , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for NCKAP1L Gene

Genomics for NCKAP1L Gene

Regulatory Elements for NCKAP1L Gene

Genomic Location for NCKAP1L Gene

54,497,711 bp from pter
54,548,238 bp from pter
50,528 bases
Plus strand

Genomic View for NCKAP1L Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for NCKAP1L Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for NCKAP1L Gene

Proteins for NCKAP1L Gene

  • Protein details for NCKAP1L Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Recommended name:
    Nck-associated protein 1-like
    Protein Accession:
    Secondary Accessions:
    • B4DUT5
    • Q52LW0

    Protein attributes for NCKAP1L Gene

    1127 amino acids
    Molecular mass:
    128153 Da
    Quaternary structure:
    No Data Available
    • Sequence=AAA35964.1; Type=Erroneous initiation; Evidence={ECO:0000305};

    Alternative splice isoforms for NCKAP1L Gene


neXtProt entry for NCKAP1L Gene

Proteomics data for NCKAP1L Gene at MOPED

Post-translational modifications for NCKAP1L Gene

  • Ubiquitination at Lys 40, Lys 42, Lys 49, Lys 60, Lys 326, and Lys 349
  • Modification sites at PhosphoSitePlus

Other Protein References for NCKAP1L Gene

Antibody Products

No data available for DME Specific Peptides for NCKAP1L Gene

Domains & Families for NCKAP1L Gene

Protein Domains for NCKAP1L Gene


Graphical View of Domain Structure for InterPro Entry



  • Belongs to the HEM-1/HEM-2 family.
  • Belongs to the HEM-1/HEM-2 family.
genes like me logo Genes that share domains with NCKAP1L: view

No data available for Gene Families for NCKAP1L Gene

Function for NCKAP1L Gene

Gene Ontology (GO) - Molecular Function for NCKAP1L Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005096 GTPase activator activity IMP 16417406
GO:0005515 protein binding IPI 16417406
GO:0030295 protein kinase activator activity IMP 16417406
GO:0032403 protein complex binding IDA 16417406
genes like me logo Genes that share ontologies with NCKAP1L: view
genes like me logo Genes that share phenotypes with NCKAP1L: view

Animal Model Products

CRISPR Products

No data available for Molecular function , Enzyme Numbers (IUBMB) , Animal Models , Transcription Factor Targets and HOMER Transcription for NCKAP1L Gene

Localization for NCKAP1L Gene

Subcellular locations from UniProtKB/Swiss-Prot for NCKAP1L Gene

Cell membrane; Single-pass membrane protein; Cytoplasmic side.

Subcellular locations from

Jensen Localization Image for NCKAP1L Gene COMPARTMENTS Subcellular localization image for NCKAP1L gene
Compartment Confidence
cytosol 5
extracellular 5
plasma membrane 5

Gene Ontology (GO) - Cellular Components for NCKAP1L Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005829 cytosol TAS --
GO:0005887 integral component of plasma membrane NAS 1932118
GO:0016020 membrane IDA 19946888
GO:0031209 SCAR complex IDA 16417406
GO:0070062 extracellular exosome IDA 20458337
genes like me logo Genes that share ontologies with NCKAP1L: view

Pathways & Interactions for NCKAP1L Gene

genes like me logo Genes that share pathways with NCKAP1L: view

Pathways by source for NCKAP1L Gene

Gene Ontology (GO) - Biological Process for NCKAP1L Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0001782 B cell homeostasis ISS --
GO:0002262 myeloid cell homeostasis ISS --
GO:0006461 protein complex assembly ISS --
GO:0006935 chemotaxis IDA 16417406
GO:0007264 small GTPase mediated signal transduction TAS --
genes like me logo Genes that share ontologies with NCKAP1L: view

No data available for SIGNOR curated interactions for NCKAP1L Gene

Drugs & Compounds for NCKAP1L Gene

No Compound Related Data Available

Transcripts for NCKAP1L Gene

Unigene Clusters for NCKAP1L Gene

NCK-associated protein 1-like:
Representative Sequences:

CRISPR Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for NCKAP1L

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for NCKAP1L Gene

ExUns: 1a · 1b ^ 2 ^ 3a · 3b ^ 4 ^ 5 ^ 6 ^ 7 ^ 8 ^ 9 ^ 10 ^ 11 ^ 12 ^ 13 ^ 14 ^ 15 ^ 16a · 16b ^ 17 ^ 18a · 18b ^ 19 ^ 20 ^ 21a · 21b ^
SP1: - - -

ExUns: 22a · 22b ^ 23 ^ 24 ^ 25 ^ 26 ^ 27a · 27b ^ 28 ^ 29a · 29b ^ 30 ^ 31 ^ 32
SP1: - -

Relevant External Links for NCKAP1L Gene

GeneLoc Exon Structure for
ECgene alternative splicing isoforms for

Expression for NCKAP1L Gene

mRNA expression in normal human tissues for NCKAP1L Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for NCKAP1L Gene

This gene is overexpressed in Whole Blood (x13.2) and Spleen (x4.3).

Protein differential expression in normal tissues from HIPED for NCKAP1L Gene

This gene is overexpressed in Lymph node (25.4) and Peripheral blood mononuclear cells (10.2).

Integrated Proteomics: protein expression in normal tissues and cell lines from ProteomicsDB, PaxDb, MOPED, and MaxQB for NCKAP1L Gene

SOURCE GeneReport for Unigene cluster for NCKAP1L Gene Hs.182014

mRNA Expression by UniProt/SwissProt for NCKAP1L Gene

Tissue specificity: Expressed only in cells of hematopoietic origin
genes like me logo Genes that share expression patterns with NCKAP1L: view

Protein tissue co-expression partners for NCKAP1L Gene

- Elite partner

Primer Products

In Situ Assay Products

Orthologs for NCKAP1L Gene

This gene was present in the common ancestor of animals.

Orthologs for NCKAP1L Gene

Organism Taxonomy Gene Similarity Type Details
(Bos Taurus)
Mammalia NCKAP1L 35
  • 91.42 (n)
  • 94.41 (a)
  • 94 (a)
(Canis familiaris)
Mammalia NCKAP1L 35
  • 91.92 (n)
  • 95.91 (a)
  • 95 (a)
(Mus musculus)
Mammalia Nckap1l 35
  • 89.68 (n)
  • 94.85 (a)
Nckap1l 16
Nckap1l 36
  • 94 (a)
(Pan troglodytes)
Mammalia NCKAP1L 35
  • 99.54 (n)
  • 99.72 (a)
  • 100 (a)
(Rattus norvegicus)
Mammalia Nckap1l 35
  • 88.99 (n)
  • 94.54 (a)
(Monodelphis domestica)
Mammalia NCKAP1L 36
  • 86 (a)
(Ornithorhynchus anatinus)
Mammalia -- 36
  • 84 (a)
-- 36
  • 78 (a)
(Gallus gallus)
Aves NCKAP1L 35
  • 71.13 (n)
  • 72.23 (a)
  • 72 (a)
(Anolis carolinensis)
Reptilia -- 36
  • 68 (a)
-- 36
  • 71 (a)
tropical clawed frog
(Silurana tropicalis)
Amphibia nckap1l 35
  • 68.63 (n)
  • 72.37 (a)
(Danio rerio)
Actinopterygii Dr.26103 35
zgc:172352 35
  • 64.13 (n)
  • 61.32 (a)
  • 60 (a)
fruit fly
(Drosophila melanogaster)
Insecta Hem 36
  • 47 (a)
(Caenorhabditis elegans)
Secernentea gex-3 36
  • 36 (a)
sea squirt
(Ciona savignyi)
Ascidiacea CSA.9782 36
  • 47 (a)
Species with no ortholog for NCKAP1L:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)

Evolution for NCKAP1L Gene

Gene Tree for NCKAP1L (if available)
Gene Tree for NCKAP1L (if available)

Paralogs for NCKAP1L Gene

Paralogs for NCKAP1L Gene

(1) SIMAP similar genes for NCKAP1L Gene using alignment to 5 proteins:

genes like me logo Genes that share paralogs with NCKAP1L: view

Variants for NCKAP1L Gene

Sequence variations from dbSNP and Humsavar for NCKAP1L Gene

SNP ID Clin Chr 12 pos Sequence Context AA Info Type MAF
rs907711 -- 54,498,335(+) ATTAG(C/G)TATTT upstream-variant-2KB, intron-variant
rs1141061 -- 54,537,048(+) AATTT(C/G)TGGTG reference, missense
rs1249380 -- 54,506,602(-) aaaaa(A/T)aaaaa intron-variant
rs1249381 -- 54,506,228(-) tggga(A/G)gataa intron-variant
rs1249382 -- 54,505,745(-) gcaga(G/T)gttgc intron-variant

Structural Variations from Database of Genomic Variants (DGV) for NCKAP1L Gene

Variant ID Type Subtype PubMed ID
nsv508674 CNV Loss 20534489
nsv832421 CNV Gain 17160897
nsv525674 CNV Loss 19592680

Variation tolerance for NCKAP1L Gene

Residual Variation Intolerance Score: 5.97% of all genes are more intolerant (likely to be disease-causing)
Gene Damage Index Score: 5.31; 70.55% of all genes are more intolerant (likely to be disease-causing)

Relevant External Links for NCKAP1L Gene

HapMap Linkage Disequilibrium report

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for NCKAP1L Gene

Disorders for NCKAP1L Gene

Relevant External Links for NCKAP1L

Atlas of Genetics and Cytogenetics in Oncology and Haematology:
genes like me logo Genes that share disorders with NCKAP1L: view

No data available for MalaCards , UniProtKB/Swiss-Prot and Genatlas for NCKAP1L Gene

Publications for NCKAP1L Gene

  1. Hem-1 complexes are essential for Rac activation, actin polymerization, and myosin regulation during neutrophil chemotaxis. (PMID: 16417406) Weiner O.D. … Kirschner M.W. (PLoS Biol. 2006) 23 67
  2. Caspase-mediated cleavage of actin-binding and SH3-domain-containing proteins cortactin, HS1, and HIP-55 during apoptosis. (PMID: 11689006) Chen Y.-R. … Tan T.-H. (Biochem. Biophys. Res. Commun. 2001) 23 67
  3. Ser/Thr phosphorylation of hematopoietic specific protein 1 (HS1): implication of protein kinase CK2. (PMID: 10806407) Ruzzene M. … Pinna L.A. (Eur. J. Biochem. 2000) 23 67
  4. Hematopoietic lineage cell specific protein 1 associates with and down-regulates protein kinase CK2. (PMID: 10561491) Ruzzene M. … Pinna L.A. (FEBS Lett. 1999) 23 67
  5. Hem-1, a potential membrane protein, with expression restricted to blood cells. (PMID: 1932118) Hromas R. … Kaushansky K. (Biochim. Biophys. Acta 1991) 2 67

Products for NCKAP1L Gene

Sources for NCKAP1L Gene

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