Set Analyses:
Advanced Search

Advanced Search

 
Search By
Section (entire)
for


 



NCKAP1L Gene

protein-coding   GIFtS: 54
GCID: GC12P054891

NCK-Associated Protein 1-Like

(Previous name: hematopoietic protein 1)
(Previous symbol: HEM1)
  See related diseases
at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
About This Section

TryGeneCards Plus

Aliases
NCK-Associated Protein 1-Like1 2
HEM11 2 3 5
Hematopoietic Protein 11 2 3
Membrane-Associated Protein HEM-12 3

External Ids:    HGNC: 48621   Entrez Gene: 30712   Ensembl: ENSG000001233387   OMIM: 1411805   UniProtKB: P551603   

Export aliases for NCKAP1L gene to outside databases

Previous GC identifers: GC12P053178 GC12P051930


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
About This Section

TryGeneCards Plus

Entrez Gene summary for NCKAP1L Gene:
This gene encodes a member of the HEM family of tissue-specific transmembrane proteins which are highly conserved
from invertebrates through mammals. This gene is only expressed in hematopoietic cells. The encoded protein is a
part of the Scar/WAVE complex which plays an important role in regulating cell shape in both metazoans and
plants. Alternatively spliced transcript variants encoding different isoforms have been found.(provided by
RefSeq, May 2010)

GeneCards Summary for NCKAP1L Gene:
NCKAP1L (NCK-associated protein 1-like) is a protein-coding gene. Diseases associated with NCKAP1L include hypochromic anemia, and b-cell chronic lymphocytic leukemia. GO annotations related to this gene include Rac GTPase activator activity and protein complex binding. An important paralog of this gene is NCKAP1.




(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
About This Section

TryGeneCards Plus
RefSeq DNA sequence:
NC_000012.12  NC_018923.2  NT_029419.13  
Regulatory elements:
   Regulatory transcription factor binding sites in the NCKAP1L gene promoter:
         TBP   Pax-6   AREB6   POU2F1   FOXJ2 (long isoform)   POU2F1a   TFIID   FOXJ2   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidNCKAP1L promoter sequence
   Search Chromatin IP Primers for NCKAP1L

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat NCKAP1L


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 12q13.1   Ensembl cytogenetic band:  12q13.13   HGNC cytogenetic band: 12q13.1

NCKAP1L Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
NCKAP1L gene location

GeneLoc information about chromosome 12         GeneLoc Exon Structure

GeneLoc location for GC12P054891:  view genomic region     (about GC identifiers)

Start:
54,891,495 bp from pter      End:
54,937,726 bp from pter
Size:
46,232 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, and/or Cloud-Clone Corp.,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, and/or Cloud-Clone Corp., Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, Cloud-Clone Corp, and/or others.)
About This Section

TryGeneCards Plus

UniProtKB/Swiss-Prot: NCKPL_HUMAN, P55160 (See protein sequence)
Recommended Name: Nck-associated protein 1-like  
Size: 1127 amino acids; 128153 Da
Sequence caution: Sequence=AAA35964.1; Type=Erroneous initiation;
Secondary accessions: B4DUT5 Q52LW0
Alternative splicing: 2 isoforms:  P55160-1   P55160-2   (No experimental confirmation available)

Explore the universe of human proteins at neXtProt for NCKAP1L: NX_P55160

Explore proteomics data for NCKAP1L at MOPED

Post-translational modifications: 

  • Ubiquitination2 at Lys10, Lys14, Lys40, Lys42, Lys49, Lys60, Lys61, Lys326, Lys349
  • Modification sites at PhosphoSitePlus

  • See NCKAP1L Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins (2 alternative transcripts): 
    NP_001171905.1  NP_005328.2  

    ENSEMBL proteins: 
     ENSP00000293373   ENSP00000447246   ENSP00000445596  

    NCKAP1L Human Recombinant Protein Products:

    Browse Purified and Recombinant Proteins at EMD Millipore
    Browse R&D Systems for human recombinant proteins
    Browse recombinant and purified proteins available from Enzo Life Sciences
    Browse OriGene full length recombinant human proteins expressed in human HEK293 cells
    Browse OriGene Protein Over-expression Lysates
    OriGene Custom MassSpec
    OriGene Custom Protein Services for NCKAP1L
    GenScript Custom Purified and Recombinant Proteins Services for NCKAP1L
    Browse Sino Biological Recombinant Proteins
    Browse Sino Biological Cell Lysates
    Browse ProSpec Recombinant Proteins
    Browse Proteins at Cloud-Clone Corp.

    NCKAP1L Antibody Products:

    Browse EMD Millipore's Extensive Line of Mono- and Polyclonal Antibodies
    Browse R&D Systems for Antibodies
    OriGene Antibodies for NCKAP1L
    OriGene Custom Antibody Services for NCKAP1L
    Novus Biologicals NCKAP1L Antibodies
    Abcam antibodies for NCKAP1L
    Browse Antibodies at Cloud-Clone Corp.
    Search ThermoFisher Antibodies for NCKAP1L
    LSBio Antibodies in human, mouse, rat for NCKAP1L

    NCKAP1L Assay Products:

    Browse Kits and Assays available from EMD Millipore
    OriGene Custom Assay Services for NCKAP1L
    Browse R&D Systems for biochemical assays
    GenScript Custom Assay Services for NCKAP1L
    Browse Enzo Life Sciences for kits & assays
    Browse ELISAs at Cloud-Clone Corp.
    Browse CLIAs at Cloud-Clone Corp.


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
    About This Section

    TryGeneCards Plus
    1 InterPro protein domain:
     IPR019137 Nck-associated_protein-1

    Graphical View of Domain Structure for InterPro Entry P55160

    ProtoNet protein and cluster: P55160

    UniProtKB/Swiss-Prot: NCKPL_HUMAN, P55160
    Similarity: Belongs to the HEM-1/HEM-2 family


    NCKAP1L for domains           About GeneDecksing


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, and Vector BioLabs, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
    About This Section

    TryGeneCards Plus

         Gene Ontology (GO): Selected molecular function terms (see all 6):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0003700sequence-specific DNA binding transcription factor activity ----
    GO:0003707steroid hormone receptor activity ----
    GO:0005515protein binding IPI16417406
    GO:0030295protein kinase activator activity IMP16417406
    GO:0030675Rac GTPase activator activity IMP16417406
         
    NCKAP1L for ontologies           About GeneDecksing


    Phenotypes:
         4 GenomeRNAi human phenotypes for NCKAP1L:
     Decreased BPV1 E2 protein expr  Decreased Hepatitis C virus re  Increased HPV18 LCR reporter a  Increased S DNA content 

         11 MGI mutant phenotypes (inferred from 1 allele(MGI details for Nckap1l):
     cardiovascular system  cellular  growth/size/body  hematopoietic system  homeostasis/metabolism 
     immune system  liver/biliary system  other  renal/urinary system  reproductive system 
     respiratory system 

    NCKAP1L for phenotypes           About GeneDecksing

    Animal Models:
       inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for NCKAP1L
       inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for NCKAP1L

       genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for NCKAP1L
       genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for NCKAP1L

    miRNA
    Products:
        
    Block miRNA regulation of human, mouse, rat NCKAP1L using miScript Target Protectors
    Selected qRT-PCR Assays for microRNAs that regulate NCKAP1L (see all 11):
    hsa-miR-194* hsa-miR-99a* hsa-miR-99b* hsa-miR-1245 hsa-miR-3159 hsa-miR-889 hsa-miR-3682-3p hsa-miR-1322
    SwitchGear 3'UTR luciferase reporter plasmidNCKAP1L 3' UTR sequence
    Inhib. RNA
    Products:
        
    OriGene RNAi products in human, mouse, rat for NCKAP1L
    Predesigned siRNA for gene silencing in human, mouse, rat NCKAP1L

    Gene Editing
    Products:
    DNA2.0 Custom Protein Engineering Service for NCKAP1L

    Clone
    Products:
         
    OriGene clones in human, mouse for NCKAP1L (see all 12)
    OriGene ORF clones in mouse, rat for NCKAP1L
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector (see all 2): NCKAP1L (NM_005337)
    Browse Sino Biological Human cDNA Clones
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for NCKAP1L
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat NCKAP1L

    Cell Line
    Products:
         
    GenScript Custom overexpressing Cell Line Services for NCKAP1L
    Browse ESI BIO Cell Lines and PureStem Progenitors for NCKAP1L 
    In Situ Assay
    Products:
       

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for NCKAP1L


    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
    About This Section

    TryGeneCards Plus

    Subcellular locations from UniProtKB/Swiss-Prot
    NCKPL_HUMAN, P55160: Cell membrane; Single-pass membrane protein; Cytoplasmic side (Probable)
    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    cytosol5
    plasma membrane5
    nucleus2
    cytoskeleton1
    mitochondrion1
    peroxisome1

    Gene Ontology (GO): Selected cellular component terms (see all 6):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634nucleus ----
    GO:0005829cytosol IDA16417406
    GO:0005887integral component of plasma membrane NAS1932118
    GO:0016020membrane TAS1932118
    GO:0031209SCAR complex IDA16417406

    NCKAP1L for ontologies           About GeneDecksing


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
    About This Section

    TryGeneCards Plus

    SuperPaths for NCKAP1L About    
    See pathways by source

    SuperPathContained pathways About
    1Regulation of actin cytoskeleton
    Regulation of actin cytoskeleton0.57

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways



    1 Kegg Pathway  (Kegg details for NCKAP1L):
        Regulation of actin cytoskeleton


    NCKAP1L for pathways           About GeneDecksing

        Custom Pathway & Disease-focused RT2 Profiler PCR Arrays for NCKAP1L
    Interactions:

        Search GeneGlobe Interaction Network for NCKAP1L

    STRING Interaction Network Preview (showing 5 interactants - click image to see 19)

    Selected Interacting proteins for NCKAP1L (P551602, 3 ENSP000002933734) via UniProtKB, MINT, STRING, and/or I2D (see all 20)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    GAS7O608612, 3, ENSP000003226084MINT-72921 I2D: score=3 STRING: ENSP00000322608
    PRPF40AO754003I2D: score=2 
    ABI2ENSP000002610174STRING: ENSP00000261017
    CYFIP2ENSP000003909484STRING: ENSP00000390948
    RAC2ENSP000002490714STRING: ENSP00000249071
    About this table

    Gene Ontology (GO): Selected biological process terms (see all 31):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0001782B cell homeostasis ISS--
    GO:0002262myeloid cell homeostasis ISS--
    GO:0006461protein complex assembly ISS--
    GO:0006935chemotaxis IDA16417406
    GO:0030011maintenance of cell polarity IMP16417406

    NCKAP1L for ontologies           About GeneDecksing



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
    About This Section

    TryGeneCards Plus
    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
      Browse compounds at ApexBio 

    Browse Tocris compounds for NCKAP1L (NCKPL)



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, Primers from OriGene, and/or QIAGEN )
    About This Section

    TryGeneCards Plus

    REFSEQ mRNAs for NCKAP1L gene (2 alternative transcripts): 
    NM_001184976.1  NM_005337.4  

    Unigene Cluster for NCKAP1L:

    NCK-associated protein 1-like
    Hs.182014  [show with all ESTs]
    Unigene Representative Sequence: NM_005337
    8 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000293373(uc001sgc.4 uc010sox.2 uc010soy.2) ENST00000548221
    ENST00000547500 ENST00000545638 ENST00000552211 ENST00000548916 ENST00000549451
    ENST00000548980
    miRNA
    Products:
         
    Block miRNA regulation of human, mouse, rat NCKAP1L using miScript Target Protectors
    Selected qRT-PCR Assays for microRNAs that regulate NCKAP1L (see all 11):
    hsa-miR-194* hsa-miR-99a* hsa-miR-99b* hsa-miR-1245 hsa-miR-3159 hsa-miR-889 hsa-miR-3682-3p hsa-miR-1322
    SwitchGear 3'UTR luciferase reporter plasmidNCKAP1L 3' UTR sequence
    Inhib. RNA
    Products:
         
    OriGene RNAi products in human, mouse, rat for NCKAP1L
    Predesigned siRNA for gene silencing in human, mouse, rat NCKAP1L
    Clone
    Products:
         
    OriGene clones in human, mouse for NCKAP1L (see all 12)
    OriGene ORF clones in mouse, rat for NCKAP1L
    OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling
    GenScript: all cDNA clones in your preferred vector (see all 2): NCKAP1L (NM_005337)
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for NCKAP1L
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat NCKAP1L
    Primer
    Products:
        
    OriGene qPCR primer pairs and template standards for NCKAP1L
    OriGene qSTAR qPCR primer pairs in human, mouse for NCKAP1L
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat NCKAP1L
      QuantiTect SYBR Green Assays in human, mouse, rat NCKAP1L
      QuantiFast Probe-based Assays in human, mouse, rat NCKAP1L

    Additional mRNA sequence: 

    AK300783.1 AK314012.1 BC001604.2 BC064997.1 BC084547.1 BC093769.2 BC093771.1 M58285.1 

    12 DOTS entries:

    DT.110756  DT.95163588  DT.100841504  DT.86859689  DT.95163589  DT.92016724  DT.100694560  DT.100778358 
    DT.404958  DT.65284815  DT.95241684  DT.98082923 

    Selected AceView cDNA sequences (see all 183):

    BG256761 BX281615 AW445205 AI718434 CB054402 CF121737 BF195917 BG259380 
    BF446623 BX395542 BX395541 BP344751 BX398308 BF001814 AW452490 AA832119 
    BE397676 CA390313 AI655955 BX482901 BX482767 BM457698 CB054403 N47020 

    GeneLoc Exon Structure

    Selected Alternative Splicing Database (ASD) splice patterns (SP) for NCKAP1L (see all 6)    About this scheme

    ExUns: 1a · 1b ^ 2 ^ 3a · 3b ^ 4 ^ 5 ^ 6 ^ 7 ^ 8 ^ 9 ^ 10 ^ 11 ^ 12 ^ 13 ^ 14 ^ 15 ^ 16a · 16b ^ 17 ^ 18a · 18b ^ 19 ^ 20 ^ 21a · 21b ^
    SP1:                          -                             -                                               -                                                   
    SP2:                                                                                                                                                            
    SP3:                                                                                                                                                            
    SP4:                                                                                                                                                            
    SP5:                                                                                                                                                            

    ExUns: 22a · 22b ^ 23 ^ 24 ^ 25 ^ 26 ^ 27a · 27b ^ 28 ^ 29a · 29b ^ 30 ^ 31 ^ 32
    SP1:        -                                                     -                     
    SP2:                                                                                    
    SP3:                                                                                    
    SP4:                                                                                    
    SP5:                                                                                    


    ECgene alternative splicing isoforms for NCKAP1L

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
    About This Section

    TryGeneCards Plus

    NCKAP1L expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: TGTGAATTTT
    NCKAP1L Expression
    About this image


    NCKAP1L expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database
     selected tissues (see all 4) fully expand
     
     Blood (Cardiovascular System)    fully expand to see all 2 entries
             Plasmacytoid Dendritic cells Peripheral Blood
     
     Yolk Sac (Extraembryonic Tissues)
             Definitive Hematopoietic Stem Cells Definitive Yolk Sac
     
     Thymus (Hematopoietic System)
     
     Spleen (Hematopoietic System)
    NCKAP1L Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    NCKAP1L Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.182014

    UniProtKB/Swiss-Prot: NCKPL_HUMAN, P55160
    Tissue specificity: Expressed only in cells of hematopoietic origin

        Custom PCR Arrays for NCKAP1L
    Primer
    Products:
    OriGene qPCR primer pairs and template standards for NCKAP1L
    OriGene qSTAR qPCR primer pairs in human, mouse for NCKAP1L
    Pre-validated RT2 qPCR Primer Assay in human, mouse, rat NCKAP1L
    QuantiTect SYBR Green Assays in human, mouse, rat NCKAP1L
    QuantiFast Probe-based Assays in human, mouse, rat NCKAP1L
    In Situ
    Assay Products:
     

     
    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for NCKAP1L

    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
    About This Section

    TryGeneCards Plus

    This gene was present in the common ancestor of animals.

    Orthologs for NCKAP1L gene from Selected species (see all 14)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Nckap1l1 , 5 NCK associated protein 1 like1, 5 89.68(n)1
    94.85(a)1
      15 (58.96 cM)5
    1058551  NM_153505.41  NP_705725.11 
     1034538255 
    chicken
    (Gallus gallus)
    Aves NCKAP1L1 NCK-associated protein 1-like 71.13(n)
    72.23(a)
      425499  NM_001031339.1  NP_001026510.1 
    lizard
    (Anolis carolinensis)
    Reptilia --
    --
    Uncharacterized protein
    71(a)
    68(a)
    many → 1
    many → 1
    GL343252.1(406967-471863)
    GL343252.1(359647-399666)
    tropical clawed frog
    (Xenopus tropicalis)
    Amphibia nckap1l1 NCK-associated protein 1-like 68.63(n)
    72.37(a)
      100491829  XM_002935658.2  XP_002935704.2 
    zebrafish
    (Danio rerio)
    Actinopterygii Dr.261032 Transcribed sequence with weak similarity to protein more 74.26(n)    CD604658.1 
    fruit fly
    (Drosophila melanogaster)
    Insecta Hem6
    HEM-protein
    47(a)
    1 → many
    3L(22277454-22281295)
    worm
    (Caenorhabditis elegans)
    Secernentea gex-36
    Protein GEX-3 (gex-3) mRNA, complete cds
    36(a)
    1 → many
    IV(12404431-12408689) WBGene00001580


    ENSEMBL Gene Tree for NCKAP1L (if available)
    TreeFam Gene Tree for NCKAP1L (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
    About This Section

    TryGeneCards Plus
    Paralogs for NCKAP1L gene
    NCKAP12  
    1 SIMAP similar gene for NCKAP1L using alignment to 5 protein entries:     NCKPL_HUMAN (see all proteins):
    NCKAP1

    NCKAP1L for paralogs           About GeneDecksing



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
    About This Section

    TryGeneCards Plus

    Selected SNPs for NCKAP1L (see all 1032)    About this table    
    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 12 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1478837571,2
    C,Funtested154925604(+) GACTTC/TGGGAG 4 R W mis11Minor allele frequency- T:0.00NA 4552
    rs1152426651,2
    F--54889686(+) GATACG/AGAAAA 1 -- us2k11Minor allele frequency- A:0.03WA 118
    rs171127831,2
    C,F,H--54889698(+) GGGAAT/AGATTA 1 -- us2k1 tfbs39Minor allele frequency- A:0.06NA NS EA WA 800
    rs1477030841,2
    --54889837(+) GTTACA/GGAGTT 1 -- us2k10--------
    rs1174392371,2
    F--54889908(+) TTAAGG/CGTGAT 1 -- us2k11Minor allele frequency- C:0.01NA 120
    rs37823991,2
    C,A,H--54889939(+) TCTTGA/GTTAAT 1 -- us2k14Minor allele frequency- G:0.11CSA WA NA EA 360
    rs1408366381,2
    --54890015(+) ATTTAC/TTGAGT 1 -- us2k10--------
    rs177257371,2
    C,F,H--54890140(+) AGACTC/TGCCTA 1 -- us2k126Minor allele frequency- T:0.10NA NS EA WA 2910
    rs1423126151,2
    --54890198(+) CATCCA/GTAAAT 1 -- us2k10--------
    rs123009651,2
    H--54890328(+) gggtcC/Ttaaga 1 -- us2k14Minor allele frequency- T:0.00NS EA 418

    HapMap Linkage Disequilibrium report for NCKAP1L (54891495 - 54937726 bp)

    Structural Variations
         Database of Genomic Variants (DGV) 3 variations for NCKAP1L:    About this table    
    Variant IDTypeSubtypePubMed ID
    nsv525674CNV Loss19592680
    nsv508674CNV Loss20534489
    nsv832421CNV Gain17160897

    Site Specific Mutation Identification with PCR Assays
    SeqTarget long-range PCR primers for resequencing NCKAP1L
    DNA2.0 Custom Variant and Variant Library Synthesis for NCKAP1L

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
    About This Section

    TryGeneCards Plus
    OMIM gene information: 141180    OMIM disorders: --

    5 diseases for NCKAP1L:    
    About MalaCards
    hypochromic anemia    b-cell chronic lymphocytic leukemia    chronic lymphocytic leukemia    obesity
    leukemia

    1 disease from the University of Copenhagen DISEASES database for NCKAP1L:
    Hypochromic anemia

    NCKAP1L for disorders           About GeneDecksing


    Export disorders for NCKAP1L gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
    About This Section

    TryGeneCards Plus

    PubMed articles for NCKAP1L gene, integrated from 10 sources (see all 22):
    (articles sorted by number of sources associating them with NCKAP1L)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. The HEM proteins: a novel family of tissue-specific transmembrane proteins expressed from invertebrates through mammals with an essential function in oogenesis. (PubMed id 7643388)1, 2 Baumgartner S.... Hromas R. (J. Mol. Biol. 1995)
    2. Hem-1, a potential membrane protein, with expression restricted to blood cells. (PubMed id 1932118)1, 3 Hromas R....Kaushansky K. (Biochim. Biophys. Acta 1991)
    3. Hem-1 complexes are essential for Rac activation, actin polymerization, and myosin regulation during neutrophil chemotaxis. (PubMed id 16417406)1, 9 Weiner O.D....Kirschner M.W. (PLoS Biol. 2006)
    4. Systems-wide analysis of ubiquitylation dynamics reveals a key role for PAF15 ubiquitylation in DNA-damage bypass. (PubMed id 23000965)1 Povlsen L.K....Choudhary C. (Nat. Cell Biol. 2012)
    5. Methods for quantification of in vivo changes in protein ubiquitination following proteasome and deubiquitinase inhibition. (PubMed id 22505724)1 Udeshi N.D....Carr S.A. (amp 2012)
    6. Novel genetic loci identified for the pathophysiology of childhood obesity in the Hispanic population. (PubMed id 23251661)1 Comuzzie A.G....Butte N.F. (PLoS ONE 2012)
    7. NOTCH1 nuclear interactome reveals key regulators of its transcriptional activity and oncogenic function. (PubMed id 23022380)1 Yatim A....Benkirane M. (Mol. Cell 2012)
    8. Initial characterization of the human central proteome. (PubMed id 21269460)2 Burkard T.R.... Colinge J. (BMC Syst. Biol. 2011)
    9. A proteome-wide, quantitative survey of in vivo ubiquitylation sites reveals widespread regulatory roles. (PubMed id 21890473)1 Wagner S.A....Choudhary C. (amp 2011)
    10. Systematic and quantitative assessment of the ubiquitin-modified proteome. (PubMed id 21906983)1 Kim W....Gygi S.P. (Mol. Cell 2011)

    (in PubMed, OMIM, and NCBI Bookshelf)
    About This Section

    TryGeneCards Plus
     ANDOR
    Aliases
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
    About This Section

    TryGeneCards Plus
    Entrez Gene: 3071 HGNC: 4862 AceView: HEM1 Ensembl:ENSG00000123338 euGenes: HUgn3071
    ECgene: NCKAP1L Kegg: 3071 H-InvDB: NCKAP1L

    (According to HUGE)
    About This Section

    TryGeneCards Plus
      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
    About This Section

    TryGeneCards Plus
    NameDescription
    PharmGKB entry for NCKAP1L Pharmacogenomics, SNPs, Pathways

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
    About This Section

    TryGeneCards Plus
    Patent Information for NCKAP1L gene:
    Search GeneIP for patents involving NCKAP1L

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



    (Antibodies, recombinant proteins, and assays from EMD Millipore, R&D Systems, OriGene, QIAGEN, GenScript, Cell Signaling Technology, Novus Biologicals, Sino Biological, Enzo Life Sciences, Abcam, ProSpec, Cloud-Clone Corp., Thermo Fisher Scientific, LSBio, Gene Editing from DNA2.0. Clones from OriGene, GenScript, Sino Biological, DNA2.0, SwitchGear Genomics, Vector BioLabs, Cell lines from GenScript, and ESI BIO, PCR Arrays from QIAGEN, Drugs and/or compounds from EMD Millipore, Tocris Bioscience, Enzo Life Sciences, and/or ApexBio, In Situ Hybridization Assays from
    Advanced Cell Diagnostics, Animal models from inGenious Targeting Laboratory, genOway)
    About This Section

    TryGeneCards Plus

     
     EMD Millipore genomic analysis products

      
     Browse Antibodies   Browse Cell Culture Products  
     Browse ELISAs   Browse Flow Cytometry Kits  
     Browse Primer Pairs   Browse Enzyme Activity Assays/Reagents  
     Browse ELISpot/FluoroSpot Kits/Development Modules   Browse TFB/Immunoprecipitation Assays  
     Browse Apoptosis Detection Kits/Reagents   Browse Ubiquitin Proteasome Pathway (UPP) Assay Kits/Reagents  
     Browse DNA Damage/Repair Kits/Reagents   Browse Luminex Assays  
     Browse Cell Selection/Detection Kits/Reagents   Browse Secondary Antibodies/Controls/Staining Reagents  
     Browse Recombinant/Natural Proteins   Browse Stem Cell Products  
     Browse cDNA Clones   Browse Proteome Profiler Antibody Arrays  
     OriGene Antibodies for NCKAP1L   OriGene RNAi products in human, mouse, rat for NCKAP1L  
     OriGene qPCR primer pairs and template standards for NCKAP1L   Browse OriGene Protein Over-expression Lysates  
     OriGene Custom Mass Spec   OriGene clones in human, mouse for NCKAP1L  
     OriGene qSTAR qPCR primer pairs in human, mouse for NCKAP1L   Browse OriGene full length recombinant human proteins expressed in human HEK293 cells  
     OriGene ORF clones in mouse, rat for NCKAP1L   OriGene custom cloning services - gene synthesis, subcloning, mutagenesis, variant library, vector shuttling  
     OriGene Custom Antibody Services for NCKAP1L   OriGene Custom Protein Services for NCKAP1L  

     
     
     Block miRNA regulation of human, mouse, rat NCKAP1L using miScript Target Protectors SeqTarget long-range PCR primers for resequencing NCKAP1L
     DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat NCKAP1L Predesigned siRNA for gene silencing in human, mouse, rat NCKAP1L
     QuantiFast Probe-based Assays in human, mouse, rat NCKAP1L QuantiTect SYBR Green Assays in human, mouse, rat NCKAP1L
     Custom PCR Arrays for NCKAP1L Search Chromatin IP Primers for NCKAP1L
     Pre-validated RT2 qPCR Primer Assay in human, mouse, rat NCKAP1L  Search GeneGlobe Interaction Network for NCKAP1L
     Regulatory tfbs in NCKAP1L promoter
     GenScript Custom Purified and Recombinant Proteins Services for NCKAP1L GenScript cDNA clones with any tag delivered in your preferred vector for NCKAP1L
     GenScript Custom Assay Services for NCKAP1L GenScript Custom overexpressing Cell Line Services for NCKAP1L
     CloneReady with Over 120,000 Genes  Gene Synthesis: Any Gene in Any Vector
     Vector-based siRNA and miRNA, Ready for Transfection Gene Mutant Library, Variants up to 10^11
     Plasmid Preparation Custom Peptide Services
     Search for Antibodies & Assays

     Search Tocris compounds for NCKAP1L (NCKPL)
     Browse Sino Biological Proteins
     Browse Sino Biological Cell Lysates
     Browse Sino Biological cDNA Clones
     4000+ Proteins
     Search Sino Biological for antibodies, proteins & pathways
     Protein Production Services
     Transfection Reagents
     Protein A/G/L resins
     Isotyping reagents
     Search www.enzolifesciences.com for proteins, assays, substrates, inhibitors & antibodies

     Novus Tissue Slides
     NCKAP1L antibodies
     Antibodies for NCKAP1L
     See all of Abcam's Antibodies, Kits and Proteins for NCKAP1L
     Custom Antibody / Protein Production Service
     Bulk Purchasing
     Advantages of Rabbit Monoclonal antibodies
     Abcam protocols and scientific support
     Browse ProSpec Recombinant Proteins
     Browse Proteins at Cloud-Clone Corp.
     Browse Antibodies at Cloud-Clone Corp.
     Browse ELISAs at Cloud-Clone Corp.
     Browse CLIAs at Cloud-Clone Corp.



     Browse ESI BIO Cell Lines and PureStem Progenitors for NCKAP1L
     Gene Synthesis
     Protein Engineering
     Variant Library Synthesis
     Codon Optimization
     Protein Production and Purification
     Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for NCKAP1L
     SwitchGear 3'UTR luciferase reporter plasmids for NCKAP1L
     SwitchGear Promoter luciferase reporter plasmids for NCKAP1L
     Search ThermoFisher Antibodies for NCKAP1L
     Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat NCKAP1L
     inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for NCKAP1L
     inGenious Targeting Laboratory: Contact us about creating complex and humanized mouse models for NCKAP1L
     LSBio Antibodies in human, mouse, rat for NCKAP1L
    Customized transgenic rodents for:
     Humanization
     Biomarker expression
     Off-target effect monitoring
     Translational medicine
     Tissue-specific gene expresssion
     Time-controlled gene expresssion
     Browse compounds at ApexBio
           
    GeneCards Homepage - Last full update: 7 May 2014 - Incrementals: 9 May 2014 , 2 Jun 2014 , 26 Jun 2014 , 30 Jun 2014

    View Random Gene

    Category
    (GIFtS: )
    GIFtS Group
    The GeneCards human gene database gene index: 1 3 5 A B C D E F G H I J K L M N O P Q R S T U V W X Y Z 


    Developed at the Crown Human Genome Center, Department of Molecular Genetics, the Weizmann Institute of Science

    Hot genes      Disease genes      NCKAP1L gene at Home site.
    Version: 3.12.166 28 Aug 2014
    hostname: 356980-web2.xennexinc.com index build: 126 solr: 1.4