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NCK1 Gene

protein-coding   GIFtS: 68
GCID: GC03P136581

NCK Adaptor Protein 1


(Previous symbol: NCK)
  See NCK1-related disease
at  

(According to 1HGNC, 2Entrez Gene,
3UniProtKB/Swiss-Prot, 4UniProtKB/TrEMBL, 5OMIM, 6GeneLoc, 7Ensembl, 8DME, 9miRBase, 10fRNAdb, 12H-InvDB, 13NCBI, 14NONCODE, and/or 15RNAdb)
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Aliases
NCK Adaptor Protein 11 2 3     Melanoma NCK Protein2
NCK1 2 3 5     NCK Tyrosine Kinase2
SH2/SH3 Adaptor Protein NCK-Alpha2 3     nck-12
NCKalpha2     Non-Catalytic Region Of Tyrosine Kinase2
Cytoplasmic Protein NCK12     Nck-13

External Ids:    HGNC: 76641   Entrez Gene: 46902   Ensembl: ENSG000001580927   OMIM: 6005085   UniProtKB: P163333   

Export aliases for NCK1 gene to outside databases

Previous GC identifers: GC03P133543 GC03P137332 GC03P137862 GC03P137901 GC03P138063 GC03P133957


(According to Entrez Gene, GeneCards, Tocris Bioscience, Wikipedia's Gene Wiki, PharmGKB,
UniProtKB/Swiss-Prot, and/or UniProtKB/TrEMBL)
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Entrez Gene summary for NCK1 Gene:
The protein encoded by this gene is one of the signaling and transforming proteins containing Src homology 2 and 3
(SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals
from receptor tyrosine kinases to downstream signal recipients such as RAS. Alternatively spliced transcript
variants encoding different isoforms have been found. (provided by RefSeq, Jun 2010)

GeneCards Summary for NCK1 Gene:
NCK1 (NCK adaptor protein 1) is a protein-coding gene. Diseases associated with NCK1 include wiskott-aldrich syndrome. GO annotations related to this gene include protein kinase inhibitor activity and receptor binding. An important paralog of this gene is GRAP.

UniProtKB/Swiss-Prot: NCK1_HUMAN, P16333
Function: Adapter protein which associates with tyrosine-phosphorylated growth factor receptors, such as KDR and
PDGFRB, or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its
dephosphorylation by PP1. Plays a role in the DNA damage response, not in the detection of the damage by ATM/ATR,
but for efficient activation of downstream effectors, such as that of CHEK2. Plays a role in ELK1-dependent
transcriptional activation in response to activated Ras signaling. Modulates the activation of EIF2AK2/PKR by
dsRNA

Gene Wiki entry for NCK1 Gene


(According to GeneLoc and/or HGNC, and/or
Entrez Gene (NCBI build 37),
and/or miRBase,
Genomic Views according to UCSC (hg19) and Ensembl (release 75), Regulatory elements and Epigenetics data according to QIAGEN, and/or SwitchGear Genomics)
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RefSeq DNA sequence at NCBI GenBank:
NC_000003.11  NT_005612.17  NC_018914.2  
Regulatory elements:
   Regulatory transcription factor binding sites in the NCK1 gene promoter:
         HFH-3   AML1a   AP-1   ATF-2   FOXD1   C/EBPalpha   Evi-1   AREB6   FOXI1   c-Jun   
         Other transcription factors

SwitchGear Promoter luciferase reporter plasmidNCK1 promoter sequence
   Search Chromatin IP Primers for NCK1

Epigenetics:
DNA Methylation CpG Assay Predesigned for Pyrosequencing in human, mouse, rat NCK1


Genomic Location:
Genomic View: UCSC Golden Path with GeneCards custom track

Entrez Gene cytogenetic band: 3q21   Ensembl cytogenetic band:  3q22.3   HGNC cytogenetic band: 3q21

NCK1 Gene in genomic location: bands according to Ensembl, locations according to (and/or Entrez Gene and/or Ensembl if different)
NCK1 gene location

GeneLoc information about chromosome 3         GeneLoc Exon Structure

GeneLoc location for GC03P136581:  view genomic region     (about GC identifiers)

Start:
136,581,050 bp from pter      End:
136,668,665 bp from pter
Size:
87,616 bases      Orientation:
plus strand

(According to 1UniProtKB, HORDE, 2neXtProt, Ensembl, and/or Reactome, Modification sites according to PhosphoSitePlus, Specific Peptides from DME, RefSeq according to NCBI, PDB rendering according to OCA and/or Proteopedia, Recombinant Proteins from EMD Millipore, R&D Systems, GenScript, Enzo Life Sciences, OriGene, Novus Biologicals, Sino Biological, ProSpec, Cloud-Clone Corp., eBioscience, and/or antibodies-online,
Biochemical Assays by EMD Millipore, R&D Systems, OriGene, GenScript, Cell Signaling Technology, Enzo Life Sciences, Cloud-Clone Corp., eBioscience, and/or antibodies-online, Antibodies by EMD Millipore, R&D Systems, Cell Signaling Technology, OriGene, Novus Biologicals, Thermo Fisher Scientific, LSBio, Abcam, Cloud-Clone Corp, antibodies-online, and/or others.)
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UniProtKB/Swiss-Prot: NCK1_HUMAN, P16333 (See protein sequence)
Recommended Name: Cytoplasmic protein NCK1  
Size: 377 amino acids; 42864 Da
Subunit: Interacts (via SH2 domain and SH3 domain 2) with EGFR. Interacts with PAK1 and SOS1. Interacts (via SH3
domains) with PKN2. Associates with BLNK, PLCG1, VAV1 and NCK1 in a B-cell antigen receptor-dependent fashion.
Interacts with SOCS7. This interaction is required for nuclear import. Part of a complex containing PPP1R15B, PP1
and NCK1. Interacts with RALGPS1. Interacts with CAV2 (tyrosine phosphorylated form). Interacts with ADAM15.
Interacts with FASLG. Directly interacts with RASA1. Interacts with isoform 4 of MINK1. Interacts with FLT1
(tyrosine. phosphorylated). Interacts with KDR (tyrosine phosphorylated). Interacts (via SH2 domain) with EPHB1;
activates the JUN cascade to regulate cell adhesion. Interacts (via SH2 domain) with PDGFRB (tyrosine
phosphorylated). Interacts with the inactive form of EIF2AK2/PKR
6 PDB 3D structures from and Proteopedia for NCK1:
2CI8 (3D)        2CI9 (3D)        2CUB (3D)        2JS0 (3D)        2JS2 (3D)        2JW4 (3D)    
Secondary accessions: B7Z751 D3DNE3
Alternative splicing: 2 isoforms:  P16333-1   P16333-2   (No experimental confirmation available)

Explore the universe of human proteins at neXtProt for NCK1: NX_P16333

Explore proteomics data for NCK1 at MOPED

Post-translational modifications: 

  • Phosphorylated on Ser and Tyr residues. Phosphorylated in response to activation of EGFR and FcERI. Phosphorylated
    by activated PDGFRB1
  • Modification sites at neXtProt
  • Modification sites at PhosphoSitePlus

  • See NCK1 Protein Expression from SPIRE MOPED, PaxDB, and MaxQB

    REFSEQ proteins (2 alternative transcripts): 
    NP_001177725.1  NP_006144.1  

    ENSEMBL proteins: 
     ENSP00000288986   ENSP00000417273   ENSP00000419302   ENSP00000419677   ENSP00000418513  
     ENSP00000417729   ENSP00000419544   ENSP00000419631   ENSP00000418060  
    Reactome Protein details: P16333

    NCK1 Human Recombinant Protein Products:

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    Novus Biologicals NCK1 Proteins
    Novus Biologicals NCK1 Lysates
    Browse Sino Biological Recombinant Proteins
    Browse Sino Biological Cell Lysates
    ProSpec Recombinant Protein for NCK1
    Cloud-Clone Corp. Proteins for NCK1

     
    Search eBioscience for Proteins for NCK1 

     
    antibodies-online proteins for NCK1 (21 products) 

     
    antibodies-online peptides for NCK1

    NCK1 Antibody Products:

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    antibodies-online antibodies for NCK1 (55 products) 

    NCK1 Assay Products:

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    Cloud-Clone Corp. ELISAs for NCK1
    Cloud-Clone Corp. CLIAs for NCK1
    Search eBioscience for ELISAs for NCK1 
    antibodies-online kits for NCK1 (2 products) 


    (According to HGNC, IUPHAR, InterPro, ProtoNet, UniProtKB, and/or BLOCKS, Sets of similar genes according to GeneDecks)
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    HGNC Gene Families:
    SH2D: SH2 domain containing

    4 InterPro protein domains:
     IPR028526 NCK1
     IPR001452 SH3_domain
     IPR017304 NCK
     IPR000980 SH2

    Graphical View of Domain Structure for InterPro Entry P16333

    ProtoNet protein and cluster: P16333

    2 Blocks protein domains:
    IPB000980 SH2 domain signature
    IPB001452 SH3 domain signature


    UniProtKB/Swiss-Prot: NCK1_HUMAN, P16333
    Domain: Only the first and third SH3 domains seem to be involved in RASA1-binding; the second SH3 domain and the
    SH2 domains do not seeem to be involved
    Similarity: Contains 1 SH2 domain
    Similarity: Contains 3 SH3 domains


    Find genes that share domains with NCK1           About GenesLikeMe


    (According to 1UniProtKB, Genatlas, LifeMap Discovery™, IUBMB, and/or 2DME, Human phenotypes from GenomeRNAi, Animal models from MGI Mar 06 2013, inGenious Targeting Laboratory, genOway,
    transcription factor targeting from QIAGEN and/or HOMER, miRNA Gene Targets from miRTarBase, shRNA from OriGene, siRNAs from OriGene, QIAGEN, microRNA from QIAGEN, SwitchGear Genomics, Gene Editing from DNA2.0, Clones from OriGene, GenScript, Sino Biological, DNA2.0, Vector BioLabs and/or Addgene, Cell Lines from GenScript, ESI BIO, In Situ Hybridization Assays from Advanced Cell Diagnostics, Flow cytometry from eBioscience, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Molecular Function:

         UniProtKB/Swiss-Prot Summary: NCK1_HUMAN, P16333
    Function: Adapter protein which associates with tyrosine-phosphorylated growth factor receptors, such as KDR and
    PDGFRB, or their cellular substrates. Maintains low levels of EIF2S1 phosphorylation by promoting its
    dephosphorylation by PP1. Plays a role in the DNA damage response, not in the detection of the damage by ATM/ATR,
    but for efficient activation of downstream effectors, such as that of CHEK2. Plays a role in ELK1-dependent
    transcriptional activation in response to activated Ras signaling. Modulates the activation of EIF2AK2/PKR by
    dsRNA

         Genatlas biochemistry entry for NCK1:
    transduction adaptor protein 1

         Gene Ontology (GO): Selected molecular function terms (see all 7):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0004860protein kinase inhibitor activity IDA18835251
    GO:0005102receptor binding IPI12110186
    GO:0005515protein binding IPI10026169
    GO:0008093cytoskeletal adaptor activity NAS12110186
    GO:0019887protein kinase regulator activity ----
         
    Find genes that share ontologies with NCK1           About GenesLikeMe


    Phenotypes:
         2 GenomeRNAi human phenotypes for NCK1:
     Decreased viability after Chla  Increased focal adhesion (FA)  

         9 MGI mutant phenotypes (inferred from 1 allele(MGI details for Nck1):
     behavior/neurological  cellular  embryogenesis  growth/size/body  homeostasis/metabolism 
     mortality/aging  nervous system  normal  renal/urinary system 

    Find genes that share phenotypes with NCK1           About GenesLikeMe

    Animal Models:
       inGenious Targeting Laboratory: Let us create your new Knockout/Knockin mouse model for NCK1
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       genOway customized KO model: permanent, tissue-specific or time-controlled inactivation for NCK1
       genOway customized Knockin model: humanization, point mutation, expression monitoring, etc. for NCK1

    miRNA
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    7 qRT-PCR Assays for microRNAs that regulate NCK1:
    hsa-miR-4264 hsa-miR-137 hsa-miR-374b hsa-miR-622 hsa-miR-590-3p hsa-miR-374a hsa-miR-4275
    SwitchGear 3'UTR luciferase reporter plasmidNCK1 3' UTR sequence
    Inhib. RNA
    Products:
        
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    Predesigned siRNA for gene silencing in human, mouse, rat NCK1

    Gene Editing
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    Clone
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    GenScript: all cDNA clones in your preferred vector (see all 2): NCK1 (NM_006153)
    Browse Sino Biological Human cDNA Clones
    DNA2.0 Custom Codon Optimized Gene Synthesis Service for NCK1
    Vector BioLabs ready-to-use adenovirus/AAV for human, mouse, rat NCK1
    Addgene plasmids for NCK1 

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    Advanced Cell Diagnostics RNAscope RNA in situ hybridization assays for NCK1


    (According to UniProtKB, COMPARTMENTS Subcellular localization database, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene.)
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    Subcellular locations from UniProtKB/Swiss-Prot
    NCK1_HUMAN, P16333: Cytoplasm. Endoplasmic reticulum. Nucleus. Note=Mostly cytoplasmic, but shuttles between the
    cytoplasm and the nucleus. Import into the nucleus requires the interaction with SOCS7. Predominantly nuclear
    following genotoxic stresses, such as UV irradiation, hydroxyurea or mitomycin C treatments
    Subcellular locations from COMPARTMENTS: 

    CompartmentConfidence
    endoplasmic reticulum5
    nucleus5
    cytosol4
    plasma membrane4
    cytoskeleton2

    Gene Ontology (GO): Selected cellular component terms (see all 6):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0005634nucleus IEA--
    GO:0005737cytoplasm NAS12110186
    GO:0005783endoplasmic reticulum IEA--
    GO:0005829cytosol TAS--
    GO:0005886plasma membrane TAS--

    Find genes that share ontologies with NCK1           About GenesLikeMe


    (SuperPaths according to PathCards, Pathways according to R&D Systems, Cell Signaling Technology, KEGG, PharmGKB, BioSystems, Sino Biological, Reactome, Tocris Bioscience, GeneGo (Thomson Reuters), QIAGEN, and/or UniProtKB, Sets of similar genes according to GeneDecks, Interaction Networks according to QIAGEN, and/or STRING, Interactions according to 1UniProtKB, 2MINT, 3I2D, and/or 4STRING, with links to IntAct and Ensembl, Ontologies according to Gene Ontology Consortium 01 Apr 2014 via Entrez Gene).
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    SuperPaths for NCK1 About   (see all 46)  
    See pathways by source

    SuperPathContained pathways About
    1Immune response IL 23 signaling pathway
    Development Angiopoietin Tie2 signaling0.38
    Angiopoietin receptor Tie2-mediated signaling0.32
    Angiopoietin-TIE2 Signaling0.36
    2Development EGFR signaling pathway
    Development EGFR signaling pathway0.57
    GDNF-Family Ligands and Receptor Interactions0.41
    Development GDNF family signaling0.45
    EGF Pathway0.39
    3ErbB signaling pathway
    ErbB signaling pathway0.52
    ErbB signaling pathway0.52
    4Development Slit Robo signaling
    Pathogenic Escherichia coli infection0.43
    Pathogenic Escherichia coli infection0.41
    5B cell receptor signaling pathway (KEGG)
    T cell receptor signaling pathway0.38
    TCR Signaling Pathway0.36


    Find genes that share SuperPaths with NCK1           About GenesLikeMe

    Pathways by source                                                                                                                                                                 See SuperPaths
    Show all pathways

    1 R&D Systems Pathway for NCK1
        VEGF - VEGF R2 Signaling Pathways

    Selected Downloadable PowerPoint Slides of GeneGlobe Pathway Central Maps for NCK1 (see all 11)
        Paxillin Interactions
    Guidance Cues and Growth Cone Motility
    Netrin Signaling
    Ephrin-Eph Signaling
    GDNF-Family Ligands and Receptor Interactions

    3 Cell Signaling Technology (CST) Pathways for NCK1
        Cytoskeletal Signaling
    Lymphocyte Signaling
    Tyrosine Kinases / Adaptors

    Selected GeneGo (Thomson Reuters) Pathways for NCK1 (see all 6)
        Development GDNF family signaling
    Development VEGF signaling via VEGFR2 - generic cascades
    Development EGFR signaling pathway
    G-protein signaling RAC1 in cellular process
    Development Angiopoietin - Tie2 signaling

    Selected BioSystems Pathways for NCK1 (see all 19)
        EGFR1 Signaling Pathway
    ErbB signaling pathway
    BDNF signaling pathway
    B Cell Receptor Signaling Pathway
    TCR Signaling Pathway

    Selected Reactome Pathways for NCK1 (see all 7)
        Nephrin interactions
    Downstream signal transduction
    Regulation of actin dynamics for phagocytic cup formation
    Antigen activates B Cell Receptor (BCR) leading to generation of second messengers
    DCC mediated attractive signaling


    4 Kegg Pathways  (Kegg details for NCK1):
        ErbB signaling pathway
    Axon guidance
    T cell receptor signaling pathway
    Pathogenic Escherichia coli infection

        Pathway & Disease-focused RT2 Profiler PCR Arrays including NCK1: 
              Insulin Signaling Pathway in human mouse rat
              Cytoskeleton Regulators in human mouse rat
              T-cell & B-cell Activation in human mouse rat

    Interactions:

        GeneGlobe Interaction Network for NCK1

    STRING Interaction Network Preview (showing 5 interactants - click image to see 25)

    Selected Interacting proteins for NCK1 (P163331, 2, 3 ENSP000002889864) via UniProtKB, MINT, STRING, and/or I2D (see all 416)
    InteractantInteraction Details
    GeneCardExternal ID(s)
    LCP2Q130941, 2, 3, ENSP000000467944EBI-389883,EBI-346946 MINT-7995411 MINT-7995531 MINT-7995426 MINT-7995458 MINT-8029667 MINT-7995504 MINT-8224277 MINT-7306527 MINT-7995396 MINT-7995442 MINT-8037396 MINT-7995380 MINT-7995488 MINT-7995474 I2D: score=7 STRING: ENSP00000046794
    CD3EP077661, 2, 3, ENSP000003545664EBI-389883,EBI-1211297 MINT-7233971 MINT-7234008 MINT-7214135 MINT-7213923 MINT-7233987 MINT-7214094 I2D: score=5 STRING: ENSP00000354566
    MAP4K1Q929181, 2, 3, ENSP000003800664EBI-389883,EBI-881 MINT-7213100 MINT-7213019 I2D: score=7 STRING: ENSP00000380066
    ASAP1Q9ULH11, 2, 3, ENSP000003502974EBI-389883,EBI-346622 MINT-7145783 I2D: score=3 STRING: ENSP00000350297
    AGERQ151091, 3EBI-389883,EBI-1646426 I2D: score=2 
    About this table

    Gene Ontology (GO): Selected biological process terms (see all 16):    About this table

    GO IDQualified GO termEvidencePubMed IDs
    GO:0006417regulation of translation IEA--
    GO:0006469negative regulation of protein kinase activity IDA18835251
    GO:0007015actin filament organization IEA--
    GO:0007172signal complex assembly NAS12110186
    GO:0007411axon guidance TAS--

    Find genes that share ontologies with NCK1           About GenesLikeMe



    (Chemical Compounds according to UniProtKB, Enzo Life Sciences, EMD Millipore, Tocris Bioscience, ApexBio, HMDB, BitterDB, and/or Novoseek, Ligands according to IUPHAR, and Drugs according to DrugBank, Enzo Life Sciences, and/or PharmGKB)
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    Browse Small Molecules at EMD Millipore
       Browse drugs & compounds from Enzo Life Sciences
      Browse compounds at ApexBio 

    Browse Tocris compounds for NCK1

    Selected Novoseek inferred chemical compound relationships for NCK1 gene (see all 11)    About this table
    Compound   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    tyrosine 72.2 79 1448108 (4), 1333047 (3), 10229072 (3), 1280326 (3) (see all 46)
    phosphotyrosine 65.1 8 9362449 (3), 15132304 (2), 8265614 (1)
    phosphatidylinositol 40.2 7 8890167 (1), 7692230 (1), 9837978 (1), 10523848 (1) (see all 7)
    serine 38.2 13 1333047 (3), 1448108 (3), 9135144 (1), 1280326 (1) (see all 7)
    threonine 36.1 8 1333047 (2), 9135144 (1), 1280326 (1), 7706279 (1) (see all 5)
    pip2 36.1 1 12147689 (1)
    proline 27.6 2 10671570 (1)
    phosphoinositide 27 4 8894489 (1), 9531977 (1)
    vanadate 19.4 2 8895336 (2)
    phenylalanine 13.4 2 10202139 (1), 11551902 (1)



    Find genes that share compounds with NCK1           About GenesLikeMe



    (Secondary structures according to fRNAdb,
    GenBank/EMBL/DDBJ Accessions according to
    Unigene (Build 236 Homo sapiens; Apr 25 2013) or GenBank,
    RefSeq according to Entrez Gene,
    DOTS (version 10), and/or AceView, transcript ids from Ensembl with links to UCSC,
    exon structure from GeneLoc, alternative splicing isoforms according to ASD and/or ECgene,
    siRNAs from OriGene, QIAGEN, shRNA from OriGene, microRNA from QIAGEN, SwitchGear Genomics,
    Tagged/untagged cDNA clones from OriGene, GenScript, DNA2.0, Vector BioLabs, and/or Addgene, Primers from OriGene, and/or QIAGEN, Flow cytometry from eBioscience )
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    REFSEQ mRNAs for NCK1 gene (2 alternative transcripts): 
    NM_001190796.1  NM_006153.4  

    Unigene Cluster for NCK1:

    NCK adaptor protein 1
    Hs.477693  [show with all ESTs]
    Unigene Representative Sequence: NM_006153
    12 Ensembl transcripts including schematic representations, and UCSC links where relevant:
    ENST00000288986(uc003erh.3) ENST00000481752 ENST00000491539 ENST00000485096
    ENST00000476286 ENST00000460960 ENST00000478862 ENST00000488930 ENST00000496489
    ENST00000469404(uc011bme.2) ENST00000467911 ENST00000482071
    miRNA
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    hsa-miR-4264 hsa-miR-137 hsa-miR-374b hsa-miR-622 hsa-miR-590-3p hsa-miR-374a hsa-miR-4275
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      QuantiTect SYBR Green Assays in human, mouse, rat NCK1
      QuantiFast Probe-based Assays in human, mouse, rat NCK1

    Additional mRNA sequence: 

    AK301460.1 AK312692.1 BC006403.2 X17576.1 

    8 DOTS entries:

    DT.413499  DT.95265172  DT.91707118  DT.100022098  DT.120842121  DT.91694270  DT.101976672  DT.120842106 

    Selected AceView cDNA sequences (see all 87):

    AL597174 AA724030 AI360552 AU107243 BF032608 NM_006153 BQ002749 CB132726 
    BG402926 BF445782 AA746252 BM975093 AI754384 BC006403 BM981906 AA278763 
    BU752149 BP339594 AI591085 AA355775 AW182810 AI433663 CB070168 BP374700 

    GeneLoc Exon Structure

    Selected Alternative Splicing Database (ASD) splice patterns (SP) for NCK1 (see all 8)    About this scheme

    ExUns: 1a · 1b · 1c · 1d ^ 2 ^ 3 ^ 4 ^ 5a · 5b · 5c ^ 6 ^ 7a · 7b · 7c · 7d · 7e ^ 8a · 8b · 8c ^ 9
    SP1:                    -     -     -     -                 -     -                                                         
    SP2:                    -           -     -                 -     -                                                         
    SP3:                          -           -                 -     -                                                         
    SP4:                          -     -     -                 -     -                                                         
    SP5:                                                        -     -                       -     -                           


    ECgene alternative splicing isoforms for NCK1

    (RNA expression data according to H-InvDB, NONCODE, miRBase, and RNAdb, Expression images according to data from BioGPS, Illumina Human BodyMap, and CGAP SAGE, Sets of similar genes according to GeneDecks, in vivo and in vitro expression data from LifeMap Discovery™, Protein expression images according to data from SPIRE 1MOPED, 2PaxDb, and 3MaxQB, plus additional links to SOURCE, and/or BioGPS, and/or UniProtKB,
    PCR Arrays from QIAGEN, Primers from OriGene, and/or QIAGEN, In Situ Hybridization Assays from Advanced Cell Diagnostics)
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    NCK1 expression in normal human tissues (normalized intensities)
    See probesets specificity/sensitivity at GeneAnnot
    About this imageBioGPS <intensity>2/3
    CGAP TAG: TTGTACAATT
    NCK1 Expression
    About this image


    NCK1 expression in embryonic tissues and stem cells    About this table
    Data from LifeMap, the Embryonic Development and Stem Cells Database
     selected tissues (see all 8) fully expand
     
     Ovary (Reproductive System)    fully expand to see all 2 entries
             Ovarian Mesenchymal Stroma Cells Ovary Interstitium
             Oviduct
     
     Brain (Nervous System)    fully expand to see all 2 entries
             Cerebral Cortex
     
     Bone (Muscoskeletal System)
             Bone Marrow
     
     Neural Tube (Nervous System)
             Primitive Spinal Cord
     
     Trophoblast (Extraembryonic Tissues)
             Trophoblast Cells Trophoblast
    NCK1 Protein expression data from MOPED1, PaxDb2 and MaxQB3    About this image

    NCK1 Protein Expression

    SOURCE GeneReport for Unigene cluster: Hs.477693
        Pathway & Disease-focused RT2 Profiler PCR Arrays including NCK1: 
              Insulin Signaling Pathway in human mouse rat
              Cytoskeleton Regulators in human mouse rat
              T-cell & B-cell Activation in human mouse rat

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    (Orthologs according to 1,2HomoloGene (2older version, for species not in 1newer version), 3euGenes, 4SGD , 5MGI Mar 06 2013, with possible further links to Flybase and/or WormBase, and/or 6Ensembl pan taxonomic compara , Gene Trees according to Ensembl and TreeFam)
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    This gene was present in the common ancestor of animals.

    Orthologs for NCK1 gene from Selected species (see all 14)    About this table
    Organism Taxonomic
    classification
    Gene Description Human
    Similarity
    Orthology
    Type
    Details
    mouse
    (Mus musculus)
    Mammalia Nck11 , 5 non-catalytic region of tyrosine kinase adaptor protein more1, 5 92.84(n)1
    98.94(a)1
      9 (52.69 cM)5
    179731  NM_010878.21  NP_035008.21 
     1004922935 
    African clawed frog
    (Xenopus laevis)
    Amphibia Xl.3792 Xenopus laevis SH2/SH3 adaptor protein (Nck) mRNA, more 79.58(n)    U85781.1 
    zebrafish
    (Danio rerio)
    Actinopterygii nck1b1 NCK adaptor protein 1b 68.79(n)
    77.72(a)
      100334953  XM_005162523.1  XP_005162580.1 
    fruit fly
    (Drosophila melanogaster)
    Insecta dock1 , 3 axon guidance SH3/SH2 adaptor protein3
    dreadlocks1
    43(a)3
    52.86(n)1
    51.43(a)1
      2 21D33
    332621  NM_001272916.11  NP_001259845.11 
    worm
    (Caenorhabditis elegans)
    Secernentea ZK470.5b3   -- 40(a)
    (best of 2)
      X(4184350-4185897)   --


    ENSEMBL Gene Tree for NCK1 (if available)
    TreeFam Gene Tree for NCK1 (if available)

    (Paralogs according to 1HomoloGene,
    2Ensembl, and 3SIMAP, Pseudogenes according to 4Pseudogene.org Build 68)
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    Paralogs for NCK1 gene
    GRAP2  GRAPL2  NCK22  GRAP22  GRB22  
    10 SIMAP similar genes for NCK1 using alignment to 7 protein entries:     NCK1_HUMAN (see all proteins):
    NCK2    FYN    GRB2    CSK    LYN    LCK
    EPS8L1    PTPN11    TXK    GRAPL

    Find genes that share paralogs with NCK1           About GenesLikeMe



    (SNPs/Variants according to the 1NCBI SNP Database, 2Ensembl, 3PupaSUITE, 4UniProtKB, and DNA2.0, Linkage Disequilibrium by HapMap, Structural Variations(CNVs/InDels/Inversions) from the Database of Genomic Variants, Mutations from the Human Gene Mutation Database (HGMD), the Human Cytochrome P450 Allele Nomenclature Database, and the Locus Specific Mutation Databases (LSDB), Blood group antigen gene mutations by BGMUT, Resequencing Primers, Cancer Mutation PCR Arrays and Assays, and Copy Number PCR Arrays from QIAGEN)
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    Selected SNPs for NCK1 (see all 1592)    About this table                                 

    Genomic DataTranscription Related DataAllele Frequencies
    SNP IDValidClinical
    significance
    Chr 3 posSequence#AA
    Chg
    TypeMore#Allele
    freq
    PopTotal
    sample
    More
    ----------
    rs1510586151,2
    --136579187(+) ACATAC/GAGTAG 1 -- us2k10--------
    rs64396721,2
    C,F,A,H--136579199(+) TGTTGA/CCCAGT 1 -- us2k123Minor allele frequency- C:0.31NS EA NA WA CSA 2352
    rs1410054021,2
    C--136579246(+) TACAGC/TATAAA 1 -- us2k10--------
    rs1497712471,2
    --136579623(+) ATGACC/TCAACA 1 -- us2k10--------
    rs1922432881,2
    --136579700(+) ATCTAA/GATTAC 1 -- us2k10--------
    rs776186291,2
    C,F--136579723(+) CCACAT/CGCATG 1 -- us2k11Minor allele frequency- C:0.03WA 118
    rs1844026271,2
    --136579764(+) TAAATA/GCAGAA 1 -- us2k10--------
    rs1887127861,2
    --136579765(+) AAATGA/CAGAAA 1 -- us2k10--------
    rs1811048281,2
    C--136579786(+) TTTGAA/GGCTGA 1 -- us2k10--------
    rs1845372751,2
    --136579943(+) AAACCA/GTTTCA 1 -- us2k10--------

    HapMap Linkage Disequilibrium report for NCK1 (136581050 - 136668665 bp)

    Structural Variations
         Database of Genomic Variants (DGV) 3 variations for NCK1:    About this table    
    Variant IDTypeSubtypePubMed ID
    esv2725958CNV Deletion23290073
    dgv5262n71CNV Loss21882294
    nsv877530CNV Gain21882294

    Human Gene Mutation Database (HGMD): NCK1
    Site Specific Mutation Identification with PCR Assays
    SeqTarget long-range PCR primers for resequencing NCK1
    DNA2.0 Custom Variant and Variant Library Synthesis for NCK1

    (in which this Gene is Involved, According to MalaCards, OMIM, UniProtKB, the University of Copenhagen DISEASES database, Novoseek, Genatlas, GeneTests, GAD, HuGE Navigator, and/or TGDB.)
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    OMIM gene information: 600508    OMIM disorders: --

    1 disease for NCK1:    
    About MalaCards
    wiskott-aldrich syndrome


    Find genes that share disorders with NCK1           About GenesLikeMe

    4 Novoseek inferred disease relationships for NCK1 gene    About this table

    Disease   -log (P-Val)   Hits   PubMed IDs for Articles with Shared Sentences (# sentences)
    wiskott-aldrich syndrome 81.9 7 9694849 (1), 16990791 (1), 7565724 (1), 11739662 (1) (see all 7)
    tumors 3.34 1 8649775 (1)
    leukemia 0 1 10372803 (1)
    immunodeficiency 0 2 14585966 (1), 11160719 (1)

    Genetic Association Database (GAD): NCK1
    Human Genome Epidemiology (HuGE) Navigator: NCK1 (3 documents)

    Export disorders for NCK1 gene to outside databases

    (in PubMed. Associations of this gene to articles via 1Entrez Gene, 2UniProtKB/Swiss-Prot, 3HGNC, 4GAD, 5PharmGKB, 6HMDB, 7DrugBank, 8UniProtKB/TrEMBL, 9 Novoseek, and/or 10fRNAdb)
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    PubMed articles for NCK1 gene, integrated from 10 sources (see all 252):
    (articles sorted by number of sources associating them with NCK1)
        Utopia: connect your pdf to the dynamic
    world of online information

    1. Septins regulate actin organization and cell-cycle arrest through nuclear accumulation of NCK mediated by SOCS7. (PubMed id 17803907)1, 2, 9 Kremer B.E.... Macara I.G. (Cell 2007)
    2. The SH2/SH3 domain-containing protein Nck is recognized by certain anti-phospholipase C-gamma 1 monoclonal antibodies, and its phosphorylation on tyrosine is stimulated by platelet-derived growth factor and epidermal growth factor treatment. (PubMed id 1448108)1, 2, 9 Meisenhelder J. and Hunter T. (Mol. Cell. Biol. 1992)
    3. Chromosome locations of genes encoding human signal transduction adapter proteins, Nck (NCK), Shc (SHC1), and Grb2 (GRB2). (PubMed id 7806213)1, 3, 9 Huebner K....Cannizzaro L. (Genomics 1994)
    4. Identification of Grb4/Nckbeta, a src homology 2 and 3 domain- containing adapter protein having similar binding and biological properties to Nck. (PubMed id 10026169)1, 2, 9 Braverman L.E. and Quilliam L.A. (J. Biol. Chem. 1999)
    5. A novel ligand for an SH3 domain of the adaptor protein Nck bears an SH2 domain and nuclear signaling motifs. (PubMed id 9344857)1, 2, 9 Matuoka K.... Takenawa T. (Biochem. Biophys. Res. Commun. 1997)
    6. Identification of Nck family genes, chromosomal localization, expression, and signaling specificity. (PubMed id 9737977)1, 3, 9 Chen M....Li W. (J. Biol. Chem. 1998)
    7. Src-induced phosphorylation of caveolin-2 on tyrosine 19. Phospho- caveolin-2 (Tyr(P)19) is localized near focal adhesions, remains associated with lipid rafts/caveolae, but no longer forms a high molecular mass hetero-oligomer with caveolin-1. (PubMed id 12091389)1, 2, 9 Lee H....Lisanti M.P. (J. Biol. Chem. 2002)
    8. Identification and characterization of a new family of guanine nucleotide exchange factors for the ras-related GTPase Ral. (PubMed id 10747847)1, 2, 9 Rebhun J.F.... Quilliam L.A. (J. Biol. Chem. 2000)
    9. Adaptor protein Nck1 interacts with p120 Ras GTPase-activating protein and regulates its activity. (PubMed id 21664272)1, 2 Ger M.... Valius M. (Cell. Signal. 2011)
    10. Investigation of genetic susceptibility factors for human longevity - a targeted nonsynonymous SNP study. (PubMed id 20800603)1, 4 Flachsbart F....Nebel A. (Mutat. Res. 2010)

    (in PubMed, OMIM, and NCBI Bookshelf)
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     ANDOR
    Aliases
    Disorders
    Free Text  

      Query String
    PubMed
    OMIM
    NCBI Bookshelf
      (Note: In FireFox, select the above section and copy using Ctrl-C)

    (According to Entrez Gene, HGNC, AceView, euGenes, Ensembl, miRBase, ECgene, Kegg, and/or H-InvDB)
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    Entrez Gene: 4690 HGNC: 7664 AceView: NCK1 Ensembl:ENSG00000158092 euGenes: HUgn4690
    ECgene: NCK1 Kegg: 4690 H-InvDB: NCK1

    (According to HUGE)
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      --

    (According to PharmGKB, ATLAS, HORDE, IMGT, LEIDEN, UniProtKB/Swiss-Prot, UniProtKB/TrEMBL, and/or others, e.g. Wikipedia and GeneReviews, via UniProtKB/Swiss-Prot)
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    NameDescription
    PharmGKB entry for NCK1 Pharmacogenomics, SNPs, Pathways
    ATLAS Chromosomes in Cancer entry for NCK1 Genetics and Cytogenetics in Oncology and Haematology

    (Patent information from GeneIP,
    Licensable technologies from WIS Yeda, Salk, Tufts,
    IP news from LifeMap Sciences, Inc.)
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    Patent Information for NCK1 gene:
    Search GeneIP for patents involving NCK1

    GeneCards and IP:
    Japan Patent Office Licenses GeneCards     European Patent Office Licenses GeneCards     Improving the IP Search



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