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Aliases for NCF2 Gene

Aliases for NCF2 Gene

  • Neutrophil Cytosolic Factor 2 2 3
  • NADPH Oxidase Activator 2 2 3 4
  • Neutrophil Cytosolic Factor 2 (65kD, Chronic Granulomatous Disease, Autosomal 2) 2 3
  • Neutrophil NADPH Oxidase Factor 2 3 4
  • 67 KDa Neutrophil Oxidase Factor 3 4
  • P67-PHOX 3 4
  • P67PHOX 3 4
  • NCF-2 3 4
  • NOXA2 3 4
  • Chronic Granulomatous Disease, Autosomal 2 3
  • Chronic Granulomatous Disease 2
  • Neutrophil Cytosol Factor 2 3
  • Autosomal 2 2

External Ids for NCF2 Gene

Previous GeneCards Identifiers for NCF2 Gene

  • GC01M181050
  • GC01M178953
  • GC01M180044
  • GC01M180764
  • GC01M180256
  • GC01M181791
  • GC01M154762

Summaries for NCF2 Gene

Entrez Gene Summary for NCF2 Gene

  • This gene encodes neutrophil cytosolic factor 2, the 67-kilodalton cytosolic subunit of the multi-protein NADPH oxidase complex found in neutrophils. This oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome. Mutations in this gene, as well as in other NADPH oxidase subunits, can result in chronic granulomatous disease, a disease that causes recurrent infections by catalase-positive organisms. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jun 2010]

GeneCards Summary for NCF2 Gene

NCF2 (Neutrophil Cytosolic Factor 2) is a Protein Coding gene. Diseases associated with NCF2 include chronic granulomatous disease due to deficiency of ncf-2 and chronic granulomatous disease. Among its related pathways are Disease and Class I MHC mediated antigen processing and presentation. GO annotations related to this gene include protein C-terminus binding and Rac GTPase binding. An important paralog of this gene is NOXA1.

UniProtKB/Swiss-Prot for NCF2 Gene

  • NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).

Gene Wiki entry for NCF2 Gene

No data available for Tocris Summary , PharmGKB "VIP" Summary , fRNAdb sequence ontologies and piRNA Summary for NCF2 Gene

Genomics for NCF2 Gene

Regulatory Elements for NCF2 Gene

Transcription factor binding sites by QIAGEN in the NCF2 gene promoter:

Genomic Location for NCF2 Gene

183,555,562 bp from pter
183,590,921 bp from pter
35,360 bases
Minus strand

Genomic View for NCF2 Gene

UCSC Golden Path with GeneCards custom track
Cytogenetic band:
Genomic Location for NCF2 Gene
GeneLoc Logo Genomic Neighborhood Exon StructureGene Density

RefSeq DNA sequence for NCF2 Gene

Proteins for NCF2 Gene

  • Protein details for NCF2 Gene (UniProtKB/Swiss-Prot)

    Protein Symbol:
    Recommended name:
    Neutrophil cytosol factor 2
    Protein Accession:
    Secondary Accessions:
    • B2R6Q1
    • B4DKQ7
    • B4DQA7
    • E9PHJ2
    • E9PHX3
    • Q2PP06
    • Q8NFC7
    • Q9BV51

    Protein attributes for NCF2 Gene

    526 amino acids
    Molecular mass:
    59762 Da
    Quaternary structure:
    • Component of an NADPH oxidase complex composed of a heterodimer formed by the membrane proteins CYBA and CYBB and the cytosolic subunits NCF1, NCF2 and NCF4. Interacts with NCF4. Interacts (via the C-terminal SH3 domain) with NCF1 (via C-terminus). Interacts with SYTL1 and RAC1. May interact with NOXO1. Interacts with S100A8 and calprotectin (S100A8/9).

    Three dimensional structures from OCA and Proteopedia for NCF2 Gene

    Alternative splice isoforms for NCF2 Gene


neXtProt entry for NCF2 Gene

Proteomics data for NCF2 Gene at MOPED

Post-translational modifications for NCF2 Gene

  • Modification sites at PhosphoSitePlus
  • Modification sites at neXtProt

Other Protein References for NCF2 Gene

Antibody Products

  • Cell Signaling Technology (CST) Antibodies for NCF2 (p67phox)

No data available for DME Specific Peptides for NCF2 Gene

Domains for NCF2 Gene

Gene Families for NCF2 Gene

  • TTC :Tetratricopeptide (TTC) repeat domain containing

Suggested Antigen Peptide Sequences for NCF2 Gene

GenScript: Design optimal peptide antigens:

Graphical View of Domain Structure for InterPro Entry



  • P19878
  • The OPR/PB1 domain mediates the association with NCF4/p40-PHOX.
  • Contains 1 PB1 domain.
  • Contains 2 SH3 domains.
  • Belongs to the NCF2/NOXA1 family.
  • Contains 3 TPR repeats.
genes like me logo Genes that share domains with NCF2: view

Function for NCF2 Gene

Molecular function for NCF2 Gene

GENATLAS Biochemistry: neutrophil cytosolic factor 2 (component of NADPH oxidase system),p675-phox,oxygen dependent mechanism of phagocytosis
UniProtKB/Swiss-Prot Function: NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).

Gene Ontology (GO) - Molecular Function for NCF2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0005515 protein binding IPI 11483497
GO:0008022 protein C-terminus binding IPI 9365277
GO:0009055 electron carrier activity TAS 1692159
GO:0048365 Rac GTPase binding IEA --
genes like me logo Genes that share ontologies with NCF2: view

Phenotypes for NCF2 Gene

genes like me logo Genes that share phenotypes with NCF2: view

Animal Model Products

miRNA for NCF2 Gene

miRTarBase miRNAs that target NCF2

No data available for Enzyme Numbers (IUBMB) , Animal Models , Transcription Factor Targeting and HOMER Transcription for NCF2 Gene

Localization for NCF2 Gene

Subcellular locations from UniProtKB/Swiss-Prot for NCF2 Gene


Subcellular locations from

Jensen Localization Image for NCF2 Gene COMPARTMENTS Subcellular localization image for NCF2 gene
Compartment Confidence
cytosol 4
lysosome 4
nucleus 4
plasma membrane 4
vacuole 4

Gene Ontology (GO) - Cellular Components for NCF2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0001669 acrosomal vesicle IEA --
GO:0005730 nucleolus IDA --
GO:0005737 cytoplasm IDA --
GO:0005829 cytosol TAS --
GO:0016020 membrane --
genes like me logo Genes that share ontologies with NCF2: view

Pathways for NCF2 Gene

genes like me logo Genes that share pathways with NCF2: view

PCR Array Products

  • Pathway & Disease-focused RT² Profiler PCR Arrays

Gene Ontology (GO) - Biological Process for NCF2 Gene

GO ID Qualified GO term Evidence PubMed IDs
GO:0002474 antigen processing and presentation of peptide antigen via MHC class I TAS --
GO:0002479 antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent TAS --
GO:0006801 superoxide metabolic process TAS 7938008
GO:0006968 cellular defense response TAS 1692159
GO:0007264 small GTPase mediated signal transduction TAS --
genes like me logo Genes that share ontologies with NCF2: view

Compounds for NCF2 Gene

(1) HMDB Compounds for NCF2 Gene

Compound Synonyms Cas Number PubMed IDs
  • 2'-(dihydrogen phosphate) 5'-(trihydrogen pyrophosphate) Adenosine 5'-ester with 1,4-dihydro-1-b-D-ribofuranosylnicotinamide

(32) Novoseek inferred chemical compound relationships for NCF2 Gene

Compound -log(P) Hits PubMed IDs
nadph 93.3 163
superoxide 82.2 50
ianbd 74.8 1
diphenyleneiodonium 71.4 1
apocynin 67.3 1
genes like me logo Genes that share compounds with NCF2: view

Transcripts for NCF2 Gene

Unigene Clusters for NCF2 Gene

Neutrophil cytosolic factor 2:
Representative Sequences:

miRNA Products

Inhibitory RNA Products

  • Predesigned siRNA for gene silencing in human,mouse,rat for NCF2

Primer Products

Flow Cytometry Products

Alternative Splicing Database (ASD) splice patterns (SP) for NCF2 Gene

ExUns: 1 ^ 2 ^ 3a · 3b ^ 4a · 4b · 4c ^ 5 ^ 6 ^ 7a · 7b · 7c ^ 8a · 8b ^ 9 ^ 10 ^ 11 ^ 12 ^ 13 ^ 14 ^ 15 ^ 16 ^ 17 ^ 18a · 18b
SP1: - - - - -
SP3: - -
SP4: - - - -
SP5: - -
SP6: - - -

Relevant External Links for NCF2 Gene

GeneLoc Exon Structure for
ECgene alternative splicing isoforms for

Expression for NCF2 Gene

mRNA expression in normal human tissues for NCF2 Gene

mRNA expression in embryonic tissues and stem cells from LifeMap Discovery

mRNA differential expression in normal tissues according to GTEx for NCF2 Gene

This gene is overexpressed in Whole Blood (42.1).

Integrated Proteomics: protein expression from ProteomicsDB, PaxDb, MOPED, and MaxQB for NCF2 Gene

SOURCE GeneReport for Unigene cluster for NCF2 Gene Hs.587558

genes like me logo Genes that share expressions with NCF2: view

In Situ Assay Products

No data available for mRNA Expression by UniProt/SwissProt for NCF2 Gene

Orthologs for NCF2 Gene

This gene was present in the common ancestor of chordates.

Orthologs for NCF2 Gene

Organism Taxonomy Gene Similarity Type Details
(Pan troglodytes)
Mammalia NCF2 35
  • 99.3 (n)
  • 98.48 (a)
NCF2 36
  • 98 (a)
(Bos Taurus)
Mammalia NCF2 35
  • 88.89 (n)
  • 89.08 (a)
NCF2 36
  • 88 (a)
(Canis familiaris)
Mammalia NCF2 35
  • 88.09 (n)
  • 86.88 (a)
NCF2 36
  • 86 (a)
(Mus musculus)
Mammalia Ncf2 35
  • 84.06 (n)
  • 84.76 (a)
Ncf2 16
Ncf2 36
  • 84 (a)
(Monodelphis domestica)
Mammalia NCF2 36
  • 77 (a)
(Ornithorhynchus anatinus)
Mammalia NCF2 36
  • 53 (a)
(Rattus norvegicus)
Mammalia Ncf2 35
  • 78.14 (n)
  • 76.02 (a)
(Gallus gallus)
Aves NCF2 35
  • 69.08 (n)
  • 64.9 (a)
NCF2 36
  • 62 (a)
(Anolis carolinensis)
Reptilia -- 36
  • 60 (a)
-- 36
  • 56 (a)
tropical clawed frog
(Silurana tropicalis)
Amphibia ncf2 35
  • 60.25 (n)
  • 55.95 (a)
(Danio rerio)
Actinopterygii ncf2 35
  • 54.92 (n)
  • 47.75 (a)
ncf2 36
  • 46 (a)
sea squirt
(Ciona savignyi)
Ascidiacea -- 36
  • 19 (a)
Species with no ortholog for NCF2:
  • A. gosspyii yeast (Ashbya gossypii)
  • Actinobacteria (Mycobacterium tuberculosis)
  • African clawed frog (Xenopus laevis)
  • African malaria mosquito (Anopheles gambiae)
  • Alicante grape (Vitis vinifera)
  • alpha proteobacteria (Wolbachia pipientis)
  • amoeba (Dictyostelium discoideum)
  • Archea (Pyrococcus horikoshii)
  • baker's yeast (Saccharomyces cerevisiae)
  • barley (Hordeum vulgare)
  • beta proteobacteria (Neisseria meningitidis)
  • bread mold (Neurospora crassa)
  • Chromalveolata (Phytophthora infestans)
  • common water flea (Daphnia pulex)
  • corn (Zea mays)
  • E. coli (Escherichia coli)
  • filamentous fungi (Aspergillus nidulans)
  • Firmicute bacteria (Streptococcus pneumoniae)
  • fission yeast (Schizosaccharomyces pombe)
  • fruit fly (Drosophila melanogaster)
  • green algae (Chlamydomonas reinhardtii)
  • honey bee (Apis mellifera)
  • K. lactis yeast (Kluyveromyces lactis)
  • loblloly pine (Pinus taeda)
  • malaria parasite (Plasmodium falciparum)
  • medicago trunc (Medicago Truncatula)
  • moss (Physcomitrella patens)
  • orangutan (Pongo pygmaeus)
  • pig (Sus scrofa)
  • rainbow trout (Oncorhynchus mykiss)
  • rice (Oryza sativa)
  • rice blast fungus (Magnaporthe grisea)
  • schistosome parasite (Schistosoma mansoni)
  • sea anemone (Nematostella vectensis)
  • sea urchin (Strongylocentrotus purpuratus)
  • sorghum (Sorghum bicolor)
  • soybean (Glycine max)
  • stem rust fungus (Puccinia graminis)
  • sugarcane (Saccharum officinarum)
  • thale cress (Arabidopsis thaliana)
  • tomato (Lycopersicon esculentum)
  • toxoplasmosis (Toxoplasma gondii)
  • Trichoplax (Trichoplax adhaerens)
  • wheat (Triticum aestivum)
  • worm (Caenorhabditis elegans)

Evolution for NCF2 Gene

Gene Tree for NCF2 (if available)
Gene Tree for NCF2 (if available)

Paralogs for NCF2 Gene

Paralogs for NCF2 Gene

Selected SIMAP similar genes for NCF2 Gene using alignment to 4 proteins:

genes like me logo Genes that share paralogs with NCF2: view

Variants for NCF2 Gene

Sequence variations from dbSNP and Humsavar for NCF2 Gene

SNP ID Clin Chr 01 pos Sequence Context AA Info Type MAF
rs699240 -- 183,585,314(+) ACAGA(C/T)CATTT intron-variant
rs699241 -- 183,577,367(+) TTTGC(A/G)CTTCA intron-variant
rs699242 -- 183,577,338(-) CCCCC(C/T)GGGCT intron-variant
rs699243 -- 183,568,799(+) CACAG(A/G)ACACC intron-variant
rs699244 -- 183,556,317(+) TTATC(A/G/T)GTCAC intron-variant

Structural Variations from Database of Genomic Variants (DGV) for NCF2 Gene

Variant ID Type Subtype PubMed ID
nsv825587 CNV Loss 20364138

Relevant External Links for NCF2 Gene

HapMap Linkage Disequilibrium report
Human Gene Mutation Database (HGMD)
Locus Specific Mutation Databases (LSDB)

No data available for Polymorphic Variants from UniProtKB/Swiss-Prot for NCF2 Gene

Disorders for NCF2 Gene

(1) OMIM Diseases for NCF2 Gene (608515)


  • Granulomatous disease, chronic, cytochrome-b-positive 2, autosomal recessive (CGD2) [MIM:233710]: A disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections. {ECO:0000269 PubMed:10498624, ECO:0000269 PubMed:10598813, ECO:0000269 PubMed:11112388, ECO:0000269 PubMed:16937026, ECO:0000269 PubMed:18625437, ECO:0000269 PubMed:19624736, ECO:0000269 PubMed:20167518, ECO:0000269 PubMed:23910690, ECO:0000269 PubMed:8286749, ECO:0000269 PubMed:9070911}. Note=The disease is caused by mutations affecting the gene represented in this entry.

(1) University of Copenhagen DISEASES for NCF2 Gene

(9) Novoseek inferred disease relationships for NCF2 Gene

Disease -log(P) Hits PubMed IDs
granulomatous disease chronic 92 39
genetic disorder 22.4 2
aspergillosis 4.65 1
necrosis 4.4 5
immunodeficiency 2.52 1

Genatlas disease for NCF2 Gene

chronic granulomatous disease,autosomal rare form

Relevant External Links for NCF2

Genetic Association Database (GAD)
Human Genome Epidemiology (HuGE) Navigator
genes like me logo Genes that share disorders with NCF2: view

Publications for NCF2 Gene

  1. Autosomal recessive chronic granulomatous disease with absence of the 67-kD cytosolic NADPH oxidase component: identification of mutation and detection of carriers. (PMID: 8286749) de Boer M. … Roos D. (Blood 1994) 3 4 23
  2. Characterization of the p67phox gene: genomic organization and restriction fragment length polymorphism analysis for prenatal diagnosis in chronic granulomatous disease. (PMID: 7903171) Kenney R.T. … Leto T.L. (Blood 1993) 3 4 23
  3. Identification of a double mutation (D160V-K161E) (sic) in the p67phox gene of a chronic granulomatous disease patient. (PMID: 9070911) Bonizzato A. … Dusi S. (Biochem. Biophys. Res. Commun. 1997) 3 4 23
  4. Molecular characterization of autosomal recessive chronic granulomatous disease caused by a defect of the nicotinamide adenine dinucleotide phosphate (reduced form) oxidase component p67-phox. (PMID: 10498624) Patino P.J. … Curnutte J.T. (Blood 1999) 3 4 23
  5. Autosomal recessive chronic granulomatous disease caused by novel mutations in NCF-2, the gene encoding the p67-phox component of phagocyte NADPH oxidase. (PMID: 10598813) Noack D. … Heyworth P.G. (Hum. Genet. 1999) 3 4 23

Products for NCF2 Gene

Sources for NCF2 Gene

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